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1.
BMC Res Notes ; 14(1): 251, 2021 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-34193263

RESUMO

OBJECTIVES: Dengue viral infection is an ongoing epidemic in Sri Lanka, causing significant mortality and morbidity. A descriptive-analytical study was carried out using serologically confirmed Dengue patients during a 6 month period. The relationship between the elevation of hepatic enzymes and severity of Dengue was assessed after stratifying recorded maximum AST/ALT (SGOT/SGPT) values 2-15 times elevated and by the phases of the illness. Sensitivity, specificity, predictive values, and ROC curves were assessed using maximum values for AST and ALT. RESULTS: Out of 255 patients, 107(42%) were females. The majority (52.9%) were in the 20-39 year age group. Only 19.6% had DHF. No statistically significant difference was noticed in the values of maximum transaminases during the febrile phase among DF and DHF patients. Higher sensitivity and low specificity with the 1-5 times elevation range was noticed, and a higher cut-off level of more than 5 times elevation showed low sensitivity and higher specificity. The combination of both transaminases cut-offs with age and sex also does not show clinically significant predictability of severe disease. The AST and ALT elevations are not showing discriminatory predictive value on dengue severity. As different serotypes cause different epidemics, it is important to carry out large-scale specific studies considering the serotypes.


Assuntos
Dengue , Dengue Grave , Adulto , Dengue/diagnóstico , Dengue/epidemiologia , Feminino , Humanos , Testes de Função Hepática , Dengue Grave/diagnóstico , Dengue Grave/epidemiologia , Sri Lanka/epidemiologia , Atenção Terciária à Saúde
2.
Artigo em Inglês | MEDLINE | ID: mdl-34199216

RESUMO

Dengue fever, a mosquito-transmitted viral disease, is present in many neighborhoods in Jeddah City, Saudi Arabia. One factor likely to affect its distribution is the socio-economic status of local neighborhoods; however, the absence of socio-economic census data in Saudi Arabia has precluded detailed investigation. This study aims to develop a proxy measure of socio-economic status in Jeddah City in order to assess its relationship with the occurrence of dengue fever. The Delphi method was used to assess the socio-economic status (high, medium or low) of local neighborhoods in Jeddah City. A Geographic Information System (GIS) was applied to understand the distribution of dengue fever according to the socio-economic status of Jeddah City neighborhoods. Low-socio-economic status neighborhoods in south Jeddah City, with poor environmental conditions and high levels of poverty and population density, reported most cases of dengue fever. Nevertheless, dengue continues to increase in high socio-economic status neighborhoods in the northern part of the city, possibly due to ideal breeding conditions caused by the presence of standing water associated with high levels of construction. Moreover, the low-socioeconomic-status neighborhoods had the highest average number of cases, being 3.95 times that of high-status neighborhoods for the period 2006-2009. The Delphi approach can produce a useful and robust measure of socio-economic status for use in the analysis of patterns of dengue fever. Results suggest that there are nuances in the relationship between socio-economic status and dengue that indicate that higher status areas are also at risk. A useful additional tool for researchers in Saudi Arabia would be the development of census data or other systematic measures that allow socio-economic status to be included in spatial analyses of dengue fever and other diseases.


Assuntos
Dengue , Animais , Cidades , Dengue/epidemiologia , Status Econômico , Sistemas de Informação Geográfica , Arábia Saudita/epidemiologia
3.
Biosensors (Basel) ; 11(7)2021 Jun 23.
Artigo em Inglês | MEDLINE | ID: mdl-34201849

RESUMO

The dengue virus (DENV) is a vector-borne flavivirus that infects around 390 million individuals each year with 2.5 billion being in danger. Having access to testing is paramount in preventing future infections and receiving adequate treatment. Currently, there are numerous conventional methods for DENV testing, such as NS1 based antigen testing, IgM/IgG antibody testing, and Polymerase Chain Reaction (PCR). In addition, novel methods are emerging that can cut both cost and time. Such methods can be effective in rural and low-income areas throughout the world. In this paper, we discuss the structural evolution of the virus followed by a comprehensive review of current dengue detection strategies and methods that are being developed or commercialized. We also discuss the state of art biosensing technologies, evaluated their performance and outline strategies to address challenges posed by the disease. Further, we outline future guidelines for the improved usage of diagnostic tools during recurrence or future outbreaks of DENV.


Assuntos
Dengue/diagnóstico , Sistemas Automatizados de Assistência Junto ao Leito , Anticorpos Antivirais , Ensaio de Imunoadsorção Enzimática , Humanos , Imunoglobulina G , Sensibilidade e Especificidade
4.
BMC Infect Dis ; 21(1): 639, 2021 Jul 02.
Artigo em Inglês | MEDLINE | ID: mdl-34215212

RESUMO

BACKGROUND: Infection by chikungunya (CHIKV) and dengue virus (DENV) can cause a wide spectrum of clinical features, many of which are undifferentiated. Cytokines, which broadly also include chemokines and growth factors, have been shown to play a role in protective immunity as well as DENV and CHIKV pathogenesis. However, differences in cytokine response to both viruses remain poorly understood, especially in patients from countries where both viruses are endemic. Our study is therefore aimed to provide a comparative profiling of cytokine response induced by acute DENV and CHIKV infections in patients with similar disease stages and in experimental in vitro infections. METHODS: By using multiplex immunoassay, we compared host cytokine profiles between acute CHIKV and DENV infections by analysing serum cytokine levels of IL-1α, IL-4, IL-5, IL-8, IL-13, RANTES, MCP-3, eotaxin, PDGF-AB/BB, and FGF-2 from the sera of acute chikungunya and dengue fever patients. We further investigated the cytokine profile responses using experimental in vitro CHIKV and DENV infections of peripheral blood mononuclear cells (PBMCs). RESULTS: We found that both CHIKV and DENV-infected patients had an upregulated level of IL-8 and IL-4, with the highest IL-4 level observed in DENV-2 infected patients. Higher IL-8 level was also correlated with lower platelet count in dengue patients. IL-13 and MCP-3 downregulation was observed only in chikungunya patients, while conversely PDGF-AB/BB and FGF-2 downregulation was unique in dengue patients. Age-associated differential expression of IL-13, MCP-3, and IL-5 was also observed, while distinct kinetics of IL-4, IL-8, and FGF-2 expression between CHIKV and DENV-infected patients were identified. Furthermore, the unique pattern of IL-8, IL-13 and MCP-3, but not IL-4 expression was also recapitulated using experimental in vitro infection in PBMCs. CONCLUSIONS: Taken together, our study identified common cytokine response profile characterized by upregulation of IL-8 and IL-4 between CHIKV and DENV infection. Downregulation of IL-13 and MCP-3 was identified as a unique cytokine response profile of acute CHIKV infection, while distinct downregulation of PDGF-AB/BB and FGF-2 characterized the response from acute DENV infection. Our study provides an important overview of the host cytokine responses between CHIKV and DENV infection, which is important to further understand the mechanism and pathology of these diseases.


Assuntos
Febre de Chikungunya/imunologia , Vírus Chikungunya/imunologia , Citocinas/metabolismo , Vírus da Dengue/imunologia , Dengue/imunologia , Adolescente , Adulto , Idoso , Febre de Chikungunya/epidemiologia , Febre de Chikungunya/metabolismo , Febre de Chikungunya/virologia , Criança , Pré-Escolar , Estudos Transversais , Citocinas/imunologia , Dengue/epidemiologia , Dengue/metabolismo , Dengue/virologia , Feminino , Humanos , Indonésia/epidemiologia , Lactente , Masculino , Pessoa de Meia-Idade , Prognóstico , Estudos Prospectivos , Adulto Jovem
5.
Emerg Med Clin North Am ; 39(3): 453-465, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34215396

RESUMO

The role of the emergency provider lies at the forefront of recognition and treatment of novel and re-emerging infectious diseases in children. Familiarity with disease presentations that might be considered rare, such as vaccine-preventable and non-endemic illnesses, is essential in identifying and controlling outbreaks. As we have seen thus far in the novel coronavirus pandemic, susceptibility, severity, transmission, and disease presentation can all have unique patterns in children. Emergency providers also have the potential to play a public health role by using lessons learned from the phenomena of vaccine hesitancy and refusal.


Assuntos
Doenças Transmissíveis Emergentes/epidemiologia , Pediatria , COVID-19/diagnóstico , COVID-19/terapia , COVID-19/transmissão , Varicela/diagnóstico , Varicela/terapia , Varicela/transmissão , Febre de Chikungunya/diagnóstico , Febre de Chikungunya/terapia , Febre de Chikungunya/transmissão , Criança , Doenças Transmissíveis Emergentes/imunologia , Árvores de Decisões , Dengue/diagnóstico , Dengue/terapia , Dengue/transmissão , Medicina de Emergência , Doença pelo Vírus Ebola/diagnóstico , Doença pelo Vírus Ebola/terapia , Doença pelo Vírus Ebola/transmissão , Humanos , Incidência , Malária/diagnóstico , Malária/terapia , Malária/transmissão , Sarampo/diagnóstico , Sarampo/terapia , Sarampo/transmissão , Papel do Médico , Saúde Pública , SARS-CoV-2 , Síndrome de Resposta Inflamatória Sistêmica , Doença Relacionada a Viagens , Vacinação , Recusa de Vacinação , Coqueluche/diagnóstico , Coqueluche/terapia , Coqueluche/transmissão , Infecção por Zika virus/diagnóstico , Infecção por Zika virus/terapia , Infecção por Zika virus/transmissão
6.
Artigo em Inglês | MEDLINE | ID: mdl-34199508

RESUMO

Aedes aegypti is the main vector of dengue globally. The variables that influence the abundance of dengue vectors are numerous and complex. This has generated a need to focus on areas at risk of disease transmission, the spatial-temporal distribution of vectors, and the factors that modulate vector abundance. To help guide and improve vector-control efforts, this study identified the ecological, social, and other environmental risk factors that affect the abundance of adult female and immature Ae. aegypti in households in urban and rural areas of northeastern Thailand. A one-year entomological study was conducted in four villages of northeastern Thailand between January and December 2019. Socio-demographic; self-reported prior dengue infections; housing conditions; durable asset ownership; water management; characteristics of water containers; knowledge, attitudes, and practices (KAP) regarding climate change and dengue; and climate data were collected. Household crowding index (HCI), premise condition index (PCI), socio-economic status (SES), and entomological indices (HI, CI, BI, and PI) were calculated. Negative binomial generalized linear models (GLMs) were fitted to identify the risk factors associated with the abundance of adult females and immature Ae. aegypti. Urban sites had higher entomological indices and numbers of adult Ae. aegypti mosquitoes than rural sites. Overall, participants' KAP about climate change and dengue were low in both settings. The fitted GLM showed that a higher abundance of adult female Ae. aegypti was significantly (p < 0.05) associated with many factors, such as a low education level of household respondents, crowded households, poor premise conditions, surrounding house density, bathrooms located indoors, unscreened windows, high numbers of wet containers, a lack of adult control, prior dengue infections, poor climate change adaptation, dengue, and vector-related practices. Many of the above were also significantly associated with a high abundance of immature mosquito stages. The GLM model also showed that maximum and mean temperature with four-and one-to-two weeks of lag were significant predictors (p < 0.05) of the abundance of adult and immature mosquitoes, respectively, in northeastern Thailand. The low KAP regarding climate change and dengue highlights the engagement needs for vector-borne disease prevention in this region. The identified risk factors are important for the critical first step toward developing routine Aedes surveillance and reliable early warning systems for effective dengue and other mosquito-borne disease prevention and control strategies at the household and community levels in this region and similar settings elsewhere.


Assuntos
Aedes , Dengue , Adulto , Animais , Aglomeração , Dengue/epidemiologia , Características da Família , Feminino , Humanos , Tailândia/epidemiologia
7.
Artigo em Inglês | MEDLINE | ID: mdl-34204166

RESUMO

Surveillance and control activities for virus-transmitting mosquitoes have primarily focused on dwellings. There is little information about viral circulation in heavily trafficked places such as schools. We collected and analyzed data to assess the presence and prevalence of dengue, chikungunya, and Zika viruses in mosquitoes, and measured Aedes indices in schools in Medellín (Colombia) between 2016-2018. In 43.27% of 2632 visits we collected Aedes adults, creating 883 pools analyzed by RT-PCR. 14.27% of pools yielded positive for dengue or Zika (infection rates of 1.75-296.29 for Aedes aegypti). Ae. aegypti was more abundant and had a higher infection rate for all studied diseases. Aedes indices varied over time. There was no association between Aedes abundance and mosquito infection rates, but the latter did correlate with cases of arboviral disease and climate. Results suggest schools are important sources of arbovirus and health agencies should include these sites in surveillance programs; it is essential to know the source for arboviral diseases transmission and the identification of the most population groups exposed to these diseases to research and developing new strategies.


Assuntos
Vírus da Dengue , Dengue , Infecção por Zika virus , Zika virus , Animais , Colômbia , Dengue/epidemiologia , Mosquitos Vetores , Instituições Acadêmicas , Infecção por Zika virus/epidemiologia
8.
Molecules ; 26(12)2021 Jun 17.
Artigo em Inglês | MEDLINE | ID: mdl-34204264

RESUMO

The present research investigated the chemical characterization and insecticidal activity of n-Hexane extracts of Epaltes divaricata (NH-EDx) along with their chief derivatives n-Hexadecanoic acid (n-HDa) and n-Octadecanoic acid (n-ODa) against the dengue vector Aedes aegypti and lepidopteran pest Spodoptera litura. Chemical screening of NH-EDx through GC-MS analysis delivered nine major derivatives, and the maximum peak area percentage was observed in n-Hexadecanoic acid (14.63%) followed by n-Octadecadienoic acid (6.73%). The larvicidal activity of NH-EDx (1000 ppm), n-HDa (5 ppm), and n-ODa (5 ppm) against the A. aegypti and S. litura larvae showed significant mortality rate in a dose-dependent way across all the instars. The larvicidal activity was profound in the A. aegypti as compared to the S. litura across all the larval instars. The sublethal dosages of NH-EDx (500 ppm), n-HDa (2.5 ppm), and n-ODa (2.5 ppm) also showed alterations in the larval/pupal durations and adult longevity in both the insect pests. The enzyme activity revealed that the α- and ß-carboxylesterase levels were decreased significantly in both the insect pests, whereas the levels of GST and CYP450 uplifted in a dose-dependent manner of NH-EDx, n-HDa, and n-ODa. Correspondingly, midgut tissues such as the epithelial layer (EL), gut lumen (GL), peritrophic matrix (Pm), and brush border membrane (BBM) were significantly altered in their morphology across both A. aegypti and S. litura against the NH-EDx and their bioactive metabolites. NH-EDx and their bioactive metabolites n-HDa and n-ODa showed significant larvicidal, growth retardant, enzyme inhibition, and midgut toxicity effects against two crucial agriculturally and medically challenging insect pest of ecological importance.


Assuntos
Aedes/efeitos dos fármacos , Asteraceae/metabolismo , Extratos Vegetais/farmacologia , Spodoptera/efeitos dos fármacos , Animais , Asteraceae/efeitos dos fármacos , Culex/efeitos dos fármacos , Dengue/prevenção & controle , Hexanos/química , Inseticidas/farmacologia , Larva/efeitos dos fármacos , Mosquitos Vetores/efeitos dos fármacos , Folhas de Planta/química , Solventes/química
9.
Rev Soc Bras Med Trop ; 54: e00102021, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34259758

RESUMO

Sickle cell intrahepatic cholestasis is a potentially fatal syndrome characterized by jaundice, painful hepatomegaly, and organ dysfunction. Two cases of sickle cell intrahepatic cholestasis associated with dengue fever were described. Endothelial damage/dysfunction is a mechanism involved in severe hepatobiliary complications related to sickle cell diseases. However, the reasons for the lack of increase in the admission of patients with sickle cell disease having severe acute hepatobiliary complications triggered by endothelial damage/dysfunction due to dengue fever remain unknown. This study describes the first association between sickle cell intrahepatic cholestasis and dengue fever.


Assuntos
Anemia Falciforme , Colestase Intra-Hepática , Dengue , Anemia Falciforme/complicações , Colestase Intra-Hepática/etiologia , Dengue/complicações , Hospitalização , Humanos , Dor
10.
BMC Med ; 19(1): 160, 2021 07 09.
Artigo em Inglês | MEDLINE | ID: mdl-34238298

RESUMO

BACKGROUND: East Africa is home to 170 million people and prone to frequent outbreaks of viral haemorrhagic fevers and various bacterial diseases. A major challenge is that epidemics mostly happen in remote areas, where infrastructure for Biosecurity Level (BSL) 3/4 laboratory capacity is not available. As samples have to be transported from the outbreak area to the National Public Health Laboratories (NPHL) in the capitals or even flown to international reference centres, diagnosis is significantly delayed and epidemics emerge. MAIN TEXT: The East African Community (EAC), an intergovernmental body of Burundi, Rwanda, Tanzania, Kenya, Uganda, and South Sudan, received 10 million € funding from the German Development Bank (KfW) to establish BSL3/4 capacity in the region. Between 2017 and 2020, the EAC in collaboration with the Bernhard-Nocht-Institute for Tropical Medicine (Germany) and the Partner Countries' Ministries of Health and their respective NPHLs, established a regional network of nine mobile BSL3/4 laboratories. These rapidly deployable laboratories allowed the region to reduce sample turn-around-time (from days to an average of 8h) at the centre of the outbreak and rapidly respond to epidemics. In the present article, the approach for implementing such a regional project is outlined and five major aspects (including recommendations) are described: (i) the overall project coordination activities through the EAC Secretariat and the Partner States, (ii) procurement of equipment, (iii) the established laboratory setup and diagnostic panels, (iv) regional training activities and capacity building of various stakeholders and (v) completed and ongoing field missions. The latter includes an EAC/WHO field simulation exercise that was conducted on the border between Tanzania and Kenya in June 2019, the support in molecular diagnosis during the Tanzanian Dengue outbreak in 2019, the participation in the Ugandan National Ebola response activities in Kisoro district along the Uganda/DRC border in Oct/Nov 2019 and the deployments of the laboratories to assist in SARS-CoV-2 diagnostics throughout the region since early 2020. CONCLUSIONS: The established EAC mobile laboratory network allows accurate and timely diagnosis of BSL3/4 pathogens in all East African countries, important for individual patient management and to effectively contain the spread of epidemic-prone diseases.


Assuntos
COVID-19/prevenção & controle , Redes Comunitárias , Dengue/epidemiologia , Doença pelo Vírus Ebola/epidemiologia , Laboratórios , Unidades Móveis de Saúde , Burundi/epidemiologia , COVID-19/terapia , Dengue/prevenção & controle , Epidemias , Doença pelo Vírus Ebola/prevenção & controle , Doença pelo Vírus Ebola/terapia , Humanos , Quênia/epidemiologia , Unidades Móveis de Saúde/economia , Saúde Pública , Ruanda/epidemiologia , SARS-CoV-2 , Sudão do Sul/epidemiologia , Tanzânia/epidemiologia , Uganda/epidemiologia
11.
Mem Inst Oswaldo Cruz ; 116: e200441, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34259736

RESUMO

BACKGROUND: A previous phylogeographic study revealed two Aedes aegypti African-related mitochondrial lineages distributed in Colombian's cities with different eco-epidemiologic characteristics with regard to dengue virus (DENV). It has been proposed these lineages might indicate independent invasion sources. OBJECTIVES: Assessing to Colombian population structure and to support evidence of its probable source origin. METHODS: We analysed a total of 267 individuals from cities of Bello, Riohacha and Villavicencio, which 241 were related to the West and East African mitochondrial lineages (termed here as WAL and EAL, respectively). Eight polymorphic microsatellite loci were analysed aiming population structure. FINDINGS: Results indicate substantial gene flow among distant and low-connected cities composing a panmictic population with incipient local differentiation of Ae. aegypti is placed in Colombia. Likewise, genetic evidence indicates no significant differences among individuals related to WAL and EAL is placed. MAIN CONCLUSIONS: Minimal genetic differentiation in low-connected Ae. aegypti populations of Colombia, and lack concordance between mitochondrial and nuclear genealogies suggest that Colombian Ae. aegypti shared a common demographic history. Under this scenario, we suggest current Ae. aegypti population structure reflects a single origin instead of contemporary migration, which founding populations have a single source from a mitochondrial polymorphic African ancient.


Assuntos
Aedes , Dengue , Aedes/genética , Animais , Colômbia , Variação Genética/genética , Humanos , Filogeografia
12.
Gac Med Mex ; 157(2): 187-193, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34270541

RESUMO

In American countries, simultaneously with the coronavirus disease 2019 (COVID-19) pandemic, epidemics caused by different arboviruses (dengue, chikungunya and Zika viruses) are occurring. In Mexico, several of the strategies to control the Aedes aegypti mosquito, which transmits arboviruses, involve the interaction of health personnel with the community. Due to the COVID-19 pandemic, social distancing and home confinement measures have been implemented. To obey these measures and avoid the risk of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) transmission, the National Center for Preventive Programs and Disease Control (CENAPRECE) has presented the vector control strategy in the scenario of simultaneous dengue and COVID-19 transmission in Mexico. In this work, we mention the routine comprehensive mosquito control measures and describe the adaptations that have been made. Furthermore, we discuss the relevance of medical personnel training and supervision, especially focusing on the similarity of symptoms between both pathologies.


Assuntos
Aedes/virologia , Infecções por Arbovirus/epidemiologia , COVID-19/epidemiologia , Monitoramento Epidemiológico , Controle de Mosquitos/métodos , Pandemias , Animais , Infecções por Arbovirus/prevenção & controle , COVID-19/prevenção & controle , Febre de Chikungunya/epidemiologia , Febre de Chikungunya/prevenção & controle , Dengue/epidemiologia , Dengue/prevenção & controle , Promoção da Saúde , Humanos , Disseminação de Informação , Distanciamento Físico , Infecção por Zika virus/epidemiologia
13.
Brasília, D.F.; OPAS; 2021-07-02. (OPAS/BRA/PHE/21-0050).
em Português | PAHO-IRIS | ID: phr2-54526

RESUMO

Com base em experiência prévia nas epidemias de dengue, a atual pandemia da COVID-19 e a persistência de casos de arbovírus em áreas endêmicas representam um desafio para os sistemas de saúde em todos os componentes e níveis, incluindo a vigilância epidemiológica. Durante a COVID-19 e a sindemia da dengue, foi relatada coinfecção pelos dois vírus em países e territórios da Região das Américas. (2, 3) De acordo com as evidências disponíveis, a gravidade e o prognóstico desses pacientes coinfectados ainda não estão claros. No entanto, será importante preparar os sistemas de saúde para responder de forma adequada e em tempo hábil a possíveis situações.


Assuntos
COVID-19 , Arbovírus , Dengue , Vírus Chikungunya , Zika virus , Coronavirus , Infecções por Coronavirus , Emergências , América , Região do Caribe
14.
Washington, D.C.; PAHO; 2021-07-02.
em Inglês, Espanhol | PAHO-IRIS | ID: phr-54497

RESUMO

In the Region of the Americas, between epidemiological week (EW) 1 and EW 22 of 2021, a total of 728,831 cases1 of arboviral disease were reported. Of those, 673,148 (92.3%) were dengue cases, 49,671 were chikungunya cases, and 6,012 were Zika cases.


En la Región de las Américas, entre la semana epidemiológica (SE) 1 y la SE 22 de 2021, se notificaron 728.831 casos1 de arbovirosis. Del total de casos, 673.148 (92,3%) fueron casos de dengue, 49.671 casos de chikungunya y 6.012 casos de Zika.


Assuntos
Arbovírus , Dengue , Vírus Chikungunya , Zika virus , COVID-19 , Infecções por Coronavirus , Regulamento Sanitário Internacional , Emergências , América , Região do Caribe , Arbovírus , Infecções por Arbovirus , Vírus Chikungunya , Zika virus , Infecções por Coronavirus , Infecção por Zika virus , Febre de Chikungunya , Emergências , Regulamento Sanitário Internacional , América , Região do Caribe
15.
Adv Exp Med Biol ; 1322: 159-193, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34258741

RESUMO

Dengue is an arthropod-borne viral disease common in subtropical and tropical regions. The widespread use of traditional medicines in these regions for dengue fever (DF) has encouraged researchers to explore the therapeutic effect of herbs and their phytochemicals in dengue infection. Phytochemicals such as quercetin, baicalein, luteolin, oxindole alkaloids, celastrol and geraniin have shown significant inhibition of dengue virus in vitro. Many phytoconstituents have better selectivity index supporting their safety profile for future development. However, in vivo studies supporting therapeutic potency for these active phytoconstituents are limited. There is a need for studies translating anti-dengue profile of active phytoconstituents to find successful anti-dengue compounds.


Assuntos
Vírus da Dengue , Dengue , Antivirais/farmacologia , Antivirais/uso terapêutico , Dengue/tratamento farmacológico , Descoberta de Drogas , Humanos , Chumbo
17.
Talanta ; 233: 122527, 2021 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-34215030

RESUMO

Dengue is one of the most commonly neglected tropical diseases transmitted by Aedes aegypti infected with Dengue virus. This virus belongs to the gender Flavivirus and produces a non-structural protein 1 (NS1), which is an important biomarker found at high levels in blood in early disease stage. Therefore, this study focused on the development of an electrochemical biosensor for NS1 detection using DNA aptamers. Gold electrodes were co-immobilized with specific aptamers and 6-mercapto-1-hexanol (MCH) to obtain a self-assembled monolayer. The molar ratio between aptamers and MCH was optimized and the platform characterized by electrochemical impedance spectroscopy and atomic force microscopy. Bovine serum albumin was added in NS1 solution to stabilize it and block the surface to avoid non-specific interactions. The biosensor performance was tested with NS1 protein serotype 4 (in phosphate saline buffer and human serum) and with a solution of serotype 1 in human serum. The results showed a sensitivity of 2.9%, 2.7% and 1.7% per decade, respectively, and low limit of detection (0.05, 0.022 and 0.025 ng/mL). The platform was also tested with Envelope protein as negative control. Furthermore, the aptamer sensor was able to detect NS1 in clinical range and it is a promising candidate for a new class for miniaturized point-of-care device for different Dengue serotypes.


Assuntos
Aptâmeros de Nucleotídeos , Técnicas Biossensoriais , Dengue , Dengue/diagnóstico , Espectroscopia Dielétrica , Eletrodos , Humanos , Limite de Detecção
18.
Recurso na Internet em Português | LIS - Localizador de Informação em Saúde | ID: lis-48251

RESUMO

Publicados na revista científica The New England Journal of Medicine, os resultados de um ensaio clínico randomizado (RCT, sigla em inglês) apontaram uma redução de 77% dos casos de dengue nas áreas que receberam o mosquito Aedes aegypti com Wolbachia, em Yogyakarta, na Indonésia.


Assuntos
Wolbachia/imunologia , Dengue/prevenção & controle , Aedes , Ensaio Clínico Controlado Aleatório
19.
Nat Commun ; 12(1): 3266, 2021 06 01.
Artigo em Inglês | MEDLINE | ID: mdl-34075032

RESUMO

The epidemic emergence of relatively rare and geographically isolated flaviviruses adds to the ongoing disease burden of viruses such as dengue. Structural analysis is key to understand and combat these pathogens. Here, we present a chimeric platform based on an insect-specific flavivirus for the safe and rapid structural analysis of pathogenic viruses. We use this approach to resolve the architecture of two neurotropic viruses and a structure of dengue virus at 2.5 Å, the highest resolution for an enveloped virion. These reconstructions allow improved modelling of the stem region of the envelope protein, revealing two lipid-like ligands within highly conserved pockets. We show that these sites are essential for viral growth and important for viral maturation. These findings define a hallmark of flavivirus virions and a potential target for broad-spectrum antivirals and vaccine design. We anticipate the chimeric platform to be widely applicable for investigating flavivirus biology.


Assuntos
Infecções por Flavivirus/terapia , Flavivirus/ultraestrutura , Proteínas do Envelope Viral/ultraestrutura , Vírion/ultraestrutura , Aedes/virologia , Animais , Antivirais/farmacologia , Antivirais/uso terapêutico , Linhagem Celular , Chlorocebus aethiops , Microscopia Crioeletrônica , Dengue/terapia , Dengue/virologia , Vacinas contra Dengue/administração & dosagem , Vacinas contra Dengue/farmacologia , Desenho de Fármacos , Flavivirus/efeitos dos fármacos , Flavivirus/imunologia , Flavivirus/patogenicidade , Infecções por Flavivirus/virologia , Humanos , Mesocricetus , Modelos Moleculares , Conformação Molecular , Mutagênese Sítio-Dirigida , Mutação Puntual , Células Vero , Proteínas do Envelope Viral/metabolismo , Vacinas Virais/farmacologia , Vacinas Virais/uso terapêutico , Vírion/efeitos dos fármacos , Vírion/metabolismo
20.
Science ; 372(6546): 1041-1042, 2021 06 04.
Artigo em Inglês | MEDLINE | ID: mdl-34083476
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