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1.
Rev Soc Bras Med Trop ; 54: e0055, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33759914

RESUMO

INTRODUCTION: Mayaro virus (MAYV) was found in Pará state, Brazil, in 1955. Since then, sporadic outbreaks have occurred in different regions of the country. METHODS: Serum sample were collected from 49 individuals in 2016 and were initially tested for dengue virus (DENV) by real-time (RT) polymerase chain reaction (PCR). DENV-negative samples were tested for MAYV and Oropouche virus (OROV) by multiplexed RT quantitative PCR. RESULTS: All samples were negative for DENV and OROV, but MAYV was detected in four samples. CONCLUSIONS: Differential diagnoses of acute febrile syndrome are required, especially in regions where several arboviruses with similar clinical manifestations are endemic.


Assuntos
Alphavirus , Arbovirus , Dengue , Alphavirus/genética , Arbovirus/genética , Brasil/epidemiologia , Dengue/diagnóstico , Dengue/epidemiologia , Surtos de Doenças , Humanos , Reação em Cadeia da Polimerase em Tempo Real , Reação em Cadeia da Polimerase Via Transcriptase Reversa
2.
Arch Argent Pediatr ; 119(2): 131-138, 2021 04.
Artigo em Espanhol | MEDLINE | ID: mdl-33749212

RESUMO

Eleven years after the first dengue outbreak in Buenos Aires, on March 20, 2020, while the mandatory quarantine for COVID-19 began dengue became the most common cause of fever consultation. The new wave of dengue cases was already among the predictions of the Pan American Health Organization based on the increase in the region of the Americas that had been occurring since the previous year. The arrival of SARS-CoV-2 at the beginning of March, added to the dengue outbreak that was already underway, made a new challenge for the health system while a new paradigm was initiated with adaptation plans to the new pandemic infection in the country. The overlapping of infections with epidemic potential such as dengue recalls the importance of not neglecting other endemic, emerging and re-emerging diseases in the shadow of the new epidemiological phenomenon.


Assuntos
/epidemiologia , Dengue/epidemiologia , Argentina/epidemiologia , Dengue/diagnóstico , Dengue/prevenção & controle , Surtos de Doenças , Política de Saúde , Humanos , Pandemias , Quarentena
3.
Virol J ; 18(1): 54, 2021 03 11.
Artigo em Inglês | MEDLINE | ID: mdl-33706767

RESUMO

BACKGROUND: The coronavirus disease 2019 (COVID-19) pandemic remains ongoing around the world, including in areas where dengue is endemic. Dengue and COVID-19, to some extent, have similar clinical and laboratory features, which can lead to misdiagnosis, delayed treatment and patient's isolation. The use of rapid diagnostic tests (RDT) is easy and convenient for fast diagnosis, however there may be issues with cross-reactivity with antibodies for other pathogens. METHODS: We assessed the possibility of cross-reactivity between SARS-CoV-2 and dengue antibodies by: (1) testing five brands of COVID-19 IgG / IgM RDTs on 60 RT-PCR-confirmed dengue samples; (2) testing 95 RT-PCR-confirmed COVID-19 samples on dengue RDT; and (3) testing samples positive for COVID-19 IgG and/or IgM on dengue RDT. RESULTS: We observed a high specificity across all five brands of COVID-19 RDTs, ranging from 98.3 to 100%. Out of the confirmed COVID-19 samples, one patient tested positive for dengue IgM only, another tested positive for dengue IgG only. One patient tested positive for dengue IgG, IgM, and NS1, suggesting a co-infection. In COVID-19 IgG and/or IgM samples, 6.3% of COVID-19 IgG-positive samples also tested positive for dengue IgG, while 21.1% of COVID-19 IgM-positive samples also tested positive for dengue IgG. CONCLUSION: Despite the high specificity of the COVID-19 RDT, we observed cross-reactions and false-positive results between dengue and COVID-19. Dengue and COVID-19 co-infection was also found. Health practitioners in dengue endemic areas should be careful when using antibody RDT for the diagnosis of dengue during the COVID-19 pandemic to avoid misdiagnosis.


Assuntos
Anticorpos Antivirais/imunologia , Reações Cruzadas/imunologia , Vírus da Dengue/imunologia , Dengue/diagnóstico , /imunologia , Adolescente , Adulto , Criança , Diagnóstico Diferencial , Testes Diagnósticos de Rotina , Reações Falso-Positivas , Feminino , Humanos , Imunoglobulina A/imunologia , Imunoglobulina G/imunologia , Imunoglobulina M/imunologia , Indonésia , Masculino , Pessoa de Meia-Idade , Sensibilidade e Especificidade , Proteínas não Estruturais Virais/imunologia , Adulto Jovem
4.
Infez Med ; 29(1): 114-116, 2021 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-33664180

RESUMO

Dengue fever should be included in the differential diagnosis of febrile illness even if another infection such as COVID-19 has been found in returning travellers from tropical and sub-tropical area where dengue virus circulates epidemically. We describe a 40-year-old man diagnosed with laboratory-confirmed COVID-19 and dengue fever during the COVID-19 outbreak in Milan, Italy.


Assuntos
/diagnóstico , Coinfecção/diagnóstico , Dengue/diagnóstico , Anamnese , Adulto , Coinfecção/virologia , Doenças Transmissíveis Importadas/diagnóstico , Doenças Transmissíveis Importadas/virologia , Vírus da Dengue , Diagnóstico Diferencial , Humanos , Masculino , Viagem
5.
Biosens Bioelectron ; 179: 113074, 2021 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-33596516

RESUMO

On global scale, the current situation of pandemic is symptomatic of increased incidences of contagious diseases caused by pathogens. The faster spread of these diseases, in a moderately short timeframe, is threatening the overall population wellbeing and conceivably the economy. The inadequacy of conventional diagnostic tools in terms of time consuming and complex laboratory-based diagnosis process is a major challenge to medical care. In present era, the development of point-of-care testing (POCT) is in demand for fast detection of infectious diseases along with "on-site" results that are helpful in timely and early action for better treatment. In addition, POCT devices also play a crucial role in preventing the transmission of infectious diseases by offering real-time testing and lab quality microbial diagnosis within minutes. Timely diagnosis and further treatment optimization facilitate the containment of outbreaks of infectious diseases. Presently, efforts are being made to support such POCT by the technological development in the field of internet of medical things (IoMT). The IoMT offers wireless-based operation and connectivity of POCT devices with health expert and medical centre. In this review, the recently developed POC diagnostics integrated or future possibilities of integration with IoMT are discussed with focus on emerging and re-emerging infectious diseases like malaria, dengue fever, influenza A (H1N1), human papilloma virus (HPV), Ebola virus disease (EVD), Zika virus (ZIKV), and coronavirus (COVID-19). The IoMT-assisted POCT systems are capable enough to fill the gap between bioinformatics generation, big rapid analytics, and clinical validation. An optimized IoMT-assisted POCT will be useful in understanding the diseases progression, treatment decision, and evaluation of efficacy of prescribed therapy.


Assuntos
Técnicas Biossensoriais/instrumentação , Doenças Transmissíveis/diagnóstico , Internet das Coisas , Testes Imediatos , Animais , Inteligência Artificial , Técnicas Biossensoriais/métodos , Infecções por Coronavirus/diagnóstico , Dengue/diagnóstico , Desenho de Equipamento , Infecções por HIV/diagnóstico , Doença pelo Vírus Ebola/diagnóstico , Humanos , Influenza Humana/diagnóstico , Malária/diagnóstico , Infecções por Orthomyxoviridae/diagnóstico , Infecção por Zika virus/diagnóstico
6.
BMC Infect Dis ; 21(1): 166, 2021 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-33568111

RESUMO

BACKGROUND: An unexpected dengue outbreak occurred in Hunan Province in 2018. This was the first dengue outbreak in this area of inland China, and 172 cases were reported. METHODS: To verify the causative agent of this outbreak and characterise the viral genes, the genes encoding the structural proteins C/prM/E of viruses isolated from local residents were sequenced followed by mutation and phylogenetic analysis. Recombination, selection pressure, potential secondary structure and three-dimensional structure analyses were also performed. RESULTS: Phylogenetic analysis revealed that all epidemic strains were of the cosmopolitan DENV-2 genotype and were most closely related to the Zhejiang strain (MH010629, 2017) and then the Malaysia strain (KJ806803, 2013). Compared with the sequence of DENV-2SS, 151 base substitutions were found in the sequences of 89 isolates; these substitutions resulted in 20 non-synonymous mutations, of which 17 mutations existed in all samples (two in the capsid protein, six in the prM/M proteins, and nine in the envelope proteins). Moreover, amino acid substitutions at the 602nd (E322:Q → H) and 670th (E390: N → S) amino acids may have enhanced the virulence of the epidemic strains. One new DNA binding site and five new protein binding sites were observed. Two polynucleotide binding sites and seven protein binding sites were lost in the epidemic strains compared with DENV-2SS. Meanwhile, five changes were found in helical regions. Minor changes were observed in helical transmembrane and disordered regions. The 429th amino acid of the E protein switched from a histamine (positively charged) to an asparagine (neutral) in all 89 isolated strains. No recombination events or positive selection pressure sites were observed. To our knowledge, this study is the first to analyse the genetic characteristics of epidemic strains in the first dengue outbreak in Hunan Province in inland China. CONCLUSIONS: The causative agent is likely to come from Zhejiang Province, a neighbouring province where dengue fever broke out in 2017. This study may help clarify the intrinsic geographical relatedness of DENV-2 and contribute to further research on pathogenicity and vaccine development.


Assuntos
Vírus da Dengue/genética , Dengue/diagnóstico , Proteínas do Envelope Viral/genética , Sítios de Ligação , Proteínas do Capsídeo/química , Proteínas do Capsídeo/genética , Proteínas do Capsídeo/metabolismo , China/epidemiologia , Dengue/epidemiologia , Dengue/virologia , Vírus da Dengue/classificação , Vírus da Dengue/isolamento & purificação , Surtos de Doenças , Genótipo , Humanos , Mutação , Filogenia , Estrutura Terciária de Proteína , RNA Viral/química , RNA Viral/metabolismo , Análise de Sequência de RNA , Proteínas do Envelope Viral/química , Proteínas não Estruturais Virais/genética , Proteínas não Estruturais Virais/metabolismo
9.
Rev Soc Bras Med Trop ; 54: e05192020, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33533817

RESUMO

INTRODUCTION: Dengue presents with a variable clinical course, ranging from mild illness to potentially fatal hemorrhage and shock. We aimed to evaluate the capabilities of various hematological parameters observed early in the course of illness for predicting the clinical outcomes of illness. METHODS: We retrospectively analyzed the records of children admitted in the pediatric inpatient services of the institute with dengue between 2017 and 2019. We determined the relationships between the hematological parameters observed during the first evaluation and the various clinical outcomes. RESULTS: We evaluated data from 613 patients (age range, 26 days to 17 years). Of these, 29.85% exhibited fever with warning signs, and 8.97% had severe dengue. Lower values of hemoglobin, platelet count, mean corpuscular volume, mean corpuscular hemoglobin concentration, and mean platelet volume, and higher values of total leukocyte count (TLC), hematocrit, and red cell distribution width variably correlated with numerous clinical outcomes-duration of hospital stay, development of complications, requirement of blood component transfusion, inotropic support, and mortality. Among the parameters, TLC ≥20,000/mL and initial platelet count ≤20,000/mL significantly associated with mortality, with odds ratios (95% confidence interval) of 11.81 (4.21-33.80) and 5.53 (1.90-16.09), respectively. CONCLUSIONS: Hematological parameters observed early during dengue infection may predict its clinical outcomes in infected children. Initial high TLC and low platelet count are potential predictors of fatal outcomes in the course of disease.


Assuntos
Dengue , Dengue Grave , Adulto , Criança , Dengue/diagnóstico , Hematócrito , Humanos , Índia/epidemiologia , Contagem de Leucócitos , Estudos Retrospectivos , Dengue Grave/diagnóstico
10.
Mem Inst Oswaldo Cruz ; 115: e200287, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33533869

RESUMO

BACKGROUND: The heat-labile nature of Dengue virus (DENV) in serum samples must be considered when applying routine diagnostic tests to avoid issues that could impact the accuracy of test results with direct implications for case management and disease reporting. OBJECTIVES: To check if pre-analytical variables, such as storage time and temperature, have an impact on the accuracy of the main routine diagnostic tests for dengue. METHODS: Virus isolation, reverse transcription real-time polymerase chain reaction (RT-PCR) and NS1 enzyme-linked immunosorbent assay (ELISA) were evaluated using 84 samples submitted to different pre-analytical conditions. FINDINGS: Sensitivity and negative predictive value were directly affected by sample storage conditions. RT-PCR and virus isolation showed greater dependence on well-conserved samples for an accurate diagnosis. Interestingly, even storage at -30ºC for a relatively short time (15 days) was not adequate for accurate results using virus isolation and RT-PCR tests. On the other hand, NS1 ELISA showed no significant reduction in positivity for aliquots tested under the same conditions as in the previous tests. MAIN CONCLUSIONS: Our results support the stability of the NS1 marker in ELISA diagnosis and indicate that the accuracy of routine tests such as virus isolation and RT-PCR is significantly affected by inadequate transport and storage conditions of serum samples.


Assuntos
Antígenos Virais/sangue , Vírus da Dengue/isolamento & purificação , Dengue/diagnóstico , Ensaio de Imunoadsorção Enzimática/métodos , Testes Imunológicos/métodos , Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos , Proteínas não Estruturais Virais/imunologia , Anticorpos Antivirais/sangue , Antígenos Virais/imunologia , Dengue/sangue , Dengue/virologia , Vírus da Dengue/genética , Vírus da Dengue/imunologia , Humanos , Valor Preditivo dos Testes , Sensibilidade e Especificidade , Proteínas não Estruturais Virais/genética
12.
BMJ Case Rep ; 14(1)2021 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-33431469

RESUMO

Bilateral sight threatening macular and optic nerve inflammation may occur with dengue fever, necessitating the use of systemic steroids. We report a case of bilateral dengue maculopathy in an elderly woman managed with targeted intravitreal steroid therapy. A 63-year-old woman presented with acute-onset painless diminution of vision in both eyes following a dengue fever episode. She had bilateral foveal inflammatory lesions, macular oedema, small vessel occlusions at the macula and scattered retinal haemorrhages and cotton-wool spots. Following systemic evaluation, intravitreal triamcinolone acetonide injection was performed in both eyes at an interval of 3 days. The foveal lesion and macular oedema resolved quickly in both eyes with a normal foveal architecture at the end of 6-week follow-up. The visual acuity improved considerably in both eyes. Inflammatory retinopathy in dengue fever may be managed with a targeted intravitreal steroid injection approach.


Assuntos
Dengue/complicações , Glucocorticoides/administração & dosagem , Edema Macular/tratamento farmacológico , Edema Macular/virologia , Triancinolona Acetonida/administração & dosagem , Dengue/diagnóstico , Dengue/terapia , Feminino , Humanos , Injeções Intravítreas , Edema Macular/diagnóstico , Pessoa de Meia-Idade
13.
BMC Infect Dis ; 21(1): 104, 2021 Jan 22.
Artigo em Inglês | MEDLINE | ID: mdl-33482756

RESUMO

BACKGROUND: Dengue virus (DENV) infection is increasingly common in southern China and can be transmitted through blood transfusion but is not currently part of donor screening throughout the region. We assessed DENV prevalence among donors at the Xishuangbanna Blood Center, Yunnan, to support development of DENV screening strategies. METHODS: Blood samples were collected randomly between June 2019 and August 2019. These were screened for anti-DENV IgG and IgM using enzyme-linked immunosorbent assay (ELISA). Then, all reactive samples and some randomly-chosen non-reactive samples were used to detect DENV RNAs using real-time polymerase-chain-reaction (RT-PCR) assays. After RT-PCR, samples were further tested for soluble nonstructural protein 1 (NS1) using the colloidal gold method. Donors demographics were also collected and assessed. RESULTS: Over the study period, 2254 donor samples were collected and tested for anti-DENV IgG and IgM by ELISA. This revealed 598 anti-DENV IgG and/or IgM reactive samples, a serological prevalence of 26.53%. Of these, 26 were RT-PCR positive and/or NS1 positive. Significant differences in DENV prevalence were noted by occupation (P = 0.001), education (P < 0.001), and ethnicity (P = 0.026). CONCLUSION: The prevalence of DENV in Xishuangbanna Blood Center was higher than most other blood centers that have implemented DENV donor screening. Our study provides first-hand data about the prevalence of DENV and allows the development of a screening strategy for clinical use.


Assuntos
Doadores de Sangue , Vírus da Dengue/isolamento & purificação , Dengue/diagnóstico , Dengue/epidemiologia , Programas de Rastreamento/métodos , Adulto , Anticorpos Antivirais/sangue , China/epidemiologia , Dengue/sangue , Vírus da Dengue/genética , Vírus da Dengue/imunologia , Feminino , Humanos , Masculino , Programas de Rastreamento/estatística & dados numéricos , Pessoa de Meia-Idade , Prevalência , RNA Viral/genética , Proteínas não Estruturais Virais/genética , Adulto Jovem
15.
Artigo em Inglês | MEDLINE | ID: mdl-33331520

RESUMO

Dengue is a viral disease, caused by an arbovirus of the genus Flavivirus. In Brazil, its incidence rate is high with a broad clinical spectrum. This report discusses a rare case of dengue associated with cutaneous leukocytoclastic vasculitis and pericardial effusion with eminence of cardiac tamponade in a previously healthy patient with no comorbidities. The serology for dengue was positive and the histopathological analysis of the cutaneous lesions confirmed the diagnosis of leukocytoclastic vasculitis. After receiving treatment, the patient's condition greatly improved.


Assuntos
Tamponamento Cardíaco/etiologia , Dengue/complicações , Derrame Pericárdico/etiologia , Vasculite Leucocitoclástica Cutânea/diagnóstico , Brasil , Tamponamento Cardíaco/diagnóstico , Dengue/diagnóstico , Humanos , Masculino , Pele , Vasculite Leucocitoclástica Cutânea/complicações , Adulto Jovem
16.
PLoS Negl Trop Dis ; 14(12): e0008960, 2020 12.
Artigo em Inglês | MEDLINE | ID: mdl-33362244

RESUMO

BACKGROUND: Dengue virus causes a wide spectrum of disease, which ranges from subclinical disease to severe dengue shock syndrome. However, estimating the risk of severe outcomes using clinical presentation or laboratory test results for rapid patient triage remains a challenge. Here, we aimed to develop prognostic models for severe dengue using machine learning, according to demographic information and clinical laboratory data of patients with dengue. METHODOLOGY/PRINCIPAL FINDINGS: Out of 1,581 patients in the National Cheng Kung University Hospital with suspected dengue infections and subjected to NS1 antigen, IgM and IgG, and qRT-PCR tests, 798 patients including 138 severe cases were enrolled in the study. The primary target outcome was severe dengue. Machine learning models were trained and tested using the patient dataset that included demographic information and qualitative laboratory test results collected on day 1 when they sought medical advice. To develop prognostic models, we applied various machine learning methods, including logistic regression, random forest, gradient boosting machine, support vector classifier, and artificial neural network, and compared the performance of the methods. The artificial neural network showed the highest average discrimination area under the receiver operating characteristic curve (0.8324 ± 0.0268) and balance accuracy (0.7523 ± 0.0273). According to the model explainer that analyzed the contributions/co-contributions of the different factors, patient age and dengue NS1 antigenemia were the two most important risk factors associated with severe dengue. Additionally, co-existence of anti-dengue IgM and IgG in patients with dengue increased the probability of severe dengue. CONCLUSIONS/SIGNIFICANCE: We developed prognostic models for the prediction of dengue severity in patients, using machine learning. The discriminative ability of the artificial neural network exhibited good performance for severe dengue prognosis. This model could help clinicians obtain a rapid prognosis during dengue outbreaks. However, the model requires further validation using external cohorts in future studies.


Assuntos
Dengue/epidemiologia , Aprendizado de Máquina , Demografia , Dengue/diagnóstico , Dengue/virologia , Feminino , Humanos , Laboratórios , Modelos Logísticos , Masculino , Pessoa de Meia-Idade , Redes Neurais de Computação , Prognóstico , Curva ROC , Fatores de Risco
17.
Viruses ; 12(12)2020 12 13.
Artigo em Inglês | MEDLINE | ID: mdl-33322218

RESUMO

The aims of this study were to determine the involvement of interleukin 17 (IL-17) and IL-17-producing cells in dengue pathogenesis. Blood samples from dengue virus (DENV)-infected patients were collected on different days after the onset of symptoms. Patients were classified according to 1997 World Health Organization guidelines. Our study examined 152 blood samples from dengue fever (DF, n = 109) and dengue hemorrhagic fever (DHF, n = 43) patients and 90 blood samples from healthy controls (HC). High serum concentrations of IL-17A and IL-22 were also associated with DHF (IL-17A [DHF vs. DF, p < 0.01; DHF vs. HC, p < 0.0001]; IL-22 [DHF vs. DF, p < 0.05; DHF vs. HC, p < 0.0001]). Moreover, there was a positive correlation between serum levels of IL-17A and IL-23, a key cytokine that promotes IL-17-based immune responses (r = 0.4089, p < 0.0001). Consistent with the IL-17-biased immune response in DHF patients, we performed ex vivo activation of peripheral blood mononuclear cells (PBMCs) from DHF patients and flow cytometry analysis showed a robust IL-17-biased immune response, characterized by a high frequency of CD4+IL-17+ producing cells. Our results suggests IL-17-producing cells and their related cytokines can play a prominent role in this viral disease.


Assuntos
Linfócitos T CD4-Positivos/metabolismo , Vírus da Dengue/fisiologia , Dengue/etiologia , Dengue/metabolismo , Interleucina-17/metabolismo , Células Th17/metabolismo , Adolescente , Adulto , Idoso , Linfócitos T CD4-Positivos/imunologia , Criança , Citocinas/sangue , Citocinas/metabolismo , Dengue/diagnóstico , Suscetibilidade a Doenças , Feminino , Humanos , Interleucina-17/sangue , Masculino , Pessoa de Meia-Idade , Índice de Gravidade de Doença , Células Th17/imunologia , Adulto Jovem
18.
J Assoc Physicians India ; 68(11): 24-27, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-33187032

RESUMO

Background: Dengue fever is the most common viral communicable disease caused by the bite of Aedes aegypti mosquito. Worldwide about 3.9 billion people are at the risk of this infection. Materials and Methods: This prospective study was done in patients of dengue fever admitted in a service hospital in the coastal area of southern India from 01 Jan 2018 to 31 Dec 2018. Results: 751 patients of confirmed dengue patients were admitted with 555 (73.9%) males and 196 (26.1%) females. The mean age was 30.6 (SD± 10.48) years, mean day of admission after the onset of illness was 3.4 days (SD±2.76). The most common presentation was fever (99.33%) followed by myalgia (77.62%), headache (67.24%), vomiting (35.41%), nausea (26.76%) and fatigue (9.05%). Bleeding diathesis was evident in 97patients (12.91%). 306 (40.75%) patients presented with warning signs. The mean duration of hospitalization was 5.73 (SD± 2.75) days. Four patients died due to severe dengue (mortality rate-0.53%). Conclusion: Intense monitoring, early detection, and management of complications can prevent mortality in dengue.


Assuntos
Dengue , Animais , Criança , Demografia , Dengue/complicações , Dengue/diagnóstico , Dengue/epidemiologia , Feminino , Humanos , Índia/epidemiologia , Masculino , Estudos Prospectivos , Centros de Atenção Terciária
19.
BMC Infect Dis ; 20(1): 835, 2020 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-33176708

RESUMO

BACKGROUND: The spatial distribution and burden of dengue in sub-Saharan Africa remains highly uncertain, despite high levels of ecological suitability. The goal of this study was to describe the epidemiology of dengue among a cohort of febrile children presenting to outpatient facilities located in areas of western Uganda with differing levels of urbanicity and malaria transmission intensity. METHODS: Eligible children were first screened for malaria using rapid diagnostic tests. Children with a negative malaria result were tested for dengue using a combination NS1/IgM/IgG rapid test (SD Bioline Dengue Duo). Confirmatory testing by RT-PCR was performed in a subset of participants. Antigen-capture ELISA was performed to estimate seroprevalence. RESULTS: Only 6 of 1416 (0.42%) children had a positive dengue rapid test, while none of the RT-PCR results were positive. ELISA testing demonstrated reactive IgG antibodies in 28 (2.2%) participants with the highest prevalence seen at the urban site in Mbarara (19 of 392, 4.9%, p < 0.001). CONCLUSIONS: Overall, these findings suggest that dengue, while present, is an uncommon cause of non-malarial, pediatric febrile illness in western Uganda. Further investigation into the eocological factors that sustain low-level transmission in urban settings are urgently needed to reduce the risk of epidemics.


Assuntos
Vírus da Dengue/genética , Vírus da Dengue/imunologia , Dengue/diagnóstico , Dengue/epidemiologia , Febre/diagnóstico , Adolescente , Criança , Pré-Escolar , Dengue/virologia , Testes Diagnósticos de Rotina/efeitos adversos , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Imunoglobulina G/imunologia , Imunoglobulina M/imunologia , Malária/diagnóstico , Malária/epidemiologia , Malária/parasitologia , Masculino , Plasmodium/imunologia , Plasmodium/isolamento & purificação , Prevalência , Estudos Prospectivos , Reação em Cadeia da Polimerase em Tempo Real , Estudos Soroepidemiológicos , Uganda/epidemiologia
20.
Sheng Wu Gong Cheng Xue Bao ; 36(10): 2206-2215, 2020 Oct 25.
Artigo em Chinês | MEDLINE | ID: mdl-33169584

RESUMO

Dengue virus (DENV) is the most widely transmitted arbovirus in the world. Due to the lack of diagnostic technology to quickly identify the virus serotypes in patients, severe dengue hemorrhagic fever cases caused by repeated infections remain high. To realize the rapid differential diagnosis of different serotypes of DENV infection by immunological methods, in this study, four DENV serotype NS1 proteins were expressed and purified in mammalian cells. Monoclonal antibodies (MAbs) against NS1 protein were obtained by hybridoma technology after immunizing BALB/c mice. Enzyme-linked immunosorbent assay, indirect immunofluorescence assay, dot blotting, and Western blotting were used to confirm the reactivity of MAbs to viral native NS1 and recombinant NS1 protein. These MAbs include not only the universal antibodies that recognize all DENV 1-4 serotype NS1, but also serotype-specific antibodies against DENV-1, DENV-2 and DENV-4. Double antibody sandwich ELISA was established based on these antibodies, which can be used to achieve rapid differential diagnosis of serotypes of DENV infection. Preparation of DENV serotype-specific MAbs and establishment of an ELISA technology for identifying DENV serotypes has laid the foundation for the rapid diagnosis of DENV clinical infection.


Assuntos
Anticorpos Antivirais , Vírus da Dengue , Dengue , Animais , Anticorpos Monoclonais , Anticorpos Antivirais/classificação , Anticorpos Antivirais/genética , Anticorpos Antivirais/metabolismo , Dengue/diagnóstico , Vírus da Dengue/imunologia , Ensaio de Imunoadsorção Enzimática , Humanos , Camundongos , Camundongos Endogâmicos BALB C , Sensibilidade e Especificidade , Sorogrupo , Proteínas não Estruturais Virais/genética , Proteínas não Estruturais Virais/imunologia
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