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1.
Braz. j. oral sci ; 20: e211060, jan.-dez. 2021. ilus
Artigo em Inglês | LILACS, BBO - Odontologia | ID: biblio-1254621

RESUMO

Aim: To evaluate the efficacy of ultrasonic bath and traditional brushing in the hygiene of complete dentures of dependent residents in long-term care institutions (LTCIs). Methods: A randomized cross-over clinical study was conducted in 17 maxillary complete denture wearers living in LTCIs. Cleaning protocols were brushing or ultrasonic bath, both applied with neutral liquid soap. Biofilm biomass were estimated by MTT reduction assay and specific microbial load (CFU) of Streptococcus spp., Staphylococcus spp. and Candida spp. were quantified by selective and differential culture media. Results: Ultrasound method showed higher percentage reduction of biofilm biomass and specific microbial loads of Streptococcus spp. compared to brushing (p<0.01). Reduction of microbial loads of the other microorganisms were not different between cleaning methods (p>0.05). Conclusions: The ultrasonic bath proved to be a feasible alternative method for the mechanical cleaning of complete dentures in LTCIs


Assuntos
Idoso , Higiene , Dentaduras , Biofilmes , Serviços de Saúde para Idosos
2.
Compend Contin Educ Dent ; 42(4): e1-e4, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-34469177

RESUMO

The wear of dentures carries with it the possibility of an inflammatory response by the denture-bearing tissues. Lack of or improper hygiene has been shown to contribute to denture stomatitis (DS). Although essential, denture hygiene is often overlooked by patients or performed improperly. As professional caregivers dentists must instruct patients and ensure that they understand the relevance and importance of denture hygiene. In light of this, the authors conducted a critical review of the literature on protocols and procedures to highlight the importance of denture hygiene and clinically show the effects of lack of denture care. A literature search was done through Google, PubMed, and Google Scholar that focused on publications published in English that dealt with denture hygiene and the disinfection process and on the protocols used. Relevant articles for protocols for denture disinfections were reviewed, and the results of different disinfection techniques were assessed, including manual, chemical, microwave, and a combination of techniques. The authors conclude that dentists must take time to instruct patients on proper denture disinfection procedures and the importance of cleaning the dentures well at least once daily. Denture hygiene and removal prior to sleeping is beneficial in preventing DS and allowing the tissue to achieve a state of homeostasis.


Assuntos
Estomatite sob Prótese , Dentaduras , Desinfecção , Humanos , Higiene , Higiene Bucal , Estomatite sob Prótese/etiologia
3.
Stomatologija ; 23(2): 48-50, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34528908

RESUMO

OBJECTIVE: To find out how the use of new acrylic full upper removable dentures changes the perception of four basic tastes: sweetness, sourness, saltiness and bitterness, and to evaluate the influence of age and gender. MATERIAL AND METHODS: This study was performed in LSMU MA Faculty of Odontology Department of Prosthodontics during 2017-09-01 - 2018-02-01. All the patients for whom new acrylic upper removable dentures were made were invited to take part. 30 patients have taken part. Sixteen solutions for basic tastes: sweetness, sourness, saltiness and bitterness; were made. The patients were examined without and with their new dentures. The patients were told to rinse an unknown solution starting from the lowest concentration and to identify the taste. Data analysis was performed using SPSS® (IBM®, Armonk, USA) version 23. RESULTS: Patients with full upper removable dentures felt sweet (p=0.002) and sour (p=0.000) taste weaker than without them, the same tendency was observed with bitter (p=0.063) and salty (p=0.059) taste. The time required to identify the taste was longer with removable dentures than without them (p<0.05). Identification of saltiness became weaker both with and without removable dentures as the age of the patients increased (p<0.05). Bitterness was identified better by women than men both with and without removable dentures (p<0.05). Age and gender had no influence on other basic tastes (p>0.05). CONCLUSION: The results of this study confirm the presumption that acrylic full upper removable dentures weaken the taste perception of the patients.


Assuntos
Percepção Gustatória , Paladar , Dentaduras , Feminino , Humanos , Masculino
4.
Geriatr Gerontol Int ; 21(10): 907-912, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34355487

RESUMO

AIM: Dentures play an important role in improving masticatory and oropharyngeal swallowing functions in some edentulous patients without dysphagia. However, few studies have been conducted on patients with dysphagia. This study investigated the effect of dentures on pharyngeal swallowing function in patients with dysphagia. METHODS: Older inpatients with dysphagia who used well-fitting dentures were included in the study. Videofluoroscopic swallowing study findings with and without dentures were compared. Pharyngeal residue and area as spatial, the distance between the maxilla and mandible, hyoid bone/laryngeal displacement, and upper esophageal sphincter opening as kinematics, oral/pharyngeal transit time as temporal measurements, and patient-reported symptoms were evaluated. The primary outcome was the pharyngeal residue measured using the normalized residue ratio scale. Comparisons were made using the paired t-test, Wilcoxon signed-rank test and Fisher's exact test. RESULTS: The mean age of the 27 participants was 86.1 ± 6.8 years. The vallecular residue was more in those without dentures (with dentures: 0.01 [0-0.02], without dentures: 0.03 [0-0.08]; P = 0.003). The pyriform sinus residue showed no significant difference. Denture removal significantly increased the pharyngeal area. The distance between the maxilla and mandible decreased in the absence of dentures, and other kinematic measurements showed no significant differences. Oral/pharyngeal transit time was prolonged without dentures. CONCLUSIONS: Morphological changes caused by the removal of dentures led to pharyngeal expansion, which may result in increased vallecular residue. A treatment plan that considers the effect of dentures on pharyngeal swallowing function may provide rehabilitation that is more effective. Geriatr Gerontol Int 2021; 21: 907-912.


Assuntos
Transtornos de Deglutição , Deglutição , Idoso , Idoso de 80 Anos ou mais , Transtornos de Deglutição/diagnóstico , Transtornos de Deglutição/etiologia , Dentaduras , Humanos , Osso Hioide , Orofaringe
5.
BMC Oral Health ; 21(1): 365, 2021 07 22.
Artigo em Inglês | MEDLINE | ID: mdl-34294054

RESUMO

BACKGROUND: The importance of efficient denture deposit removal and oral hygiene has been further underscored by the continuous increase of denture wearers. Denture hygiene management has also become an important aspect associated with denture-induced stomatitis. This study aims to evaluate the denture cleaning effect of arazyme, the metalloprotease produced from the Serratia proteamaculans HY-3. We performed growth inhibition tests against oral opportunistic pathogens to be used as a potential oral health care agent. METHODS: The proteolytic activities of arazyme was evaluated over broad ranges of temperature, pH, and denture components compared to those of subtilisin in commercially available denture cleansers. The washing effects of arazyme were also measured by using homogeneously soiled EMPA 105 cottons. To investigate the denture cleaning capability of arazyme, artificially contaminated dentures were treated with arazyme, subtilisin (Everlase 6.0T), and Polident®, respectively. The growth kinetics of Candida albicans, Enterococcus faecalis, Staphylococcus epidermis, and Streptococcus mutans were evaluated in the presence of different concentrations of arazyme to estimate the prevention effects of arazyme against major oral opportunistic pathogens. RESULTS: Arazyme showed strong proteolytic activities over wide temperature and pH ranges compared with the serine protease of the subtilisin family. Arazyme demonstrated efficient removal and decomposition of artificially contaminated dentures and showed explicit washing effects against soiled cottons. Moreover arazyme inhibited the growth of oral opportunistic pathogens, including C. albicans, E. faecalis, S. epidermis, and S. mutans, with more than 80% inhibition against C. albicans, the major cause of denture stomatitis, with 250 mg/mL arazyme. CONCLUSIONS: Arazyme shows promise as a biological oral health care agent with effective cleaning and antimicrobial activities and is a potential source for developing novel denture care agents.


Assuntos
Higienizadores de Dentadura , Serratia , Candida albicans , Higienizadores de Dentadura/farmacologia , Dentaduras , Humanos , Higiene Bucal
6.
Photodiagnosis Photodyn Ther ; 35: 102362, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34062305

RESUMO

AIM: The study aimed to assess the effect of in-vitro chlorhexidine and antimicrobial photodynamic therapy (aPDT) disinfection protocols against acrylic resin specimens colonized with S. mutans, S. aureus, E. coli, and C. albicans. MATERIAL AND METHODS: Reference strains of S. mutans, S. aureus, E. coli, and C. albicans were tested. Sixteen blocks of acrylic specimens were prepared by heat-cure acrylic resin and contaminated by in-vitro biofilm growth. Specimens in group 1, group 2 and group 3 were treated with Rose Bengal (RB), methylene blue (MB) 500 mg/L and porphyrin derivative (PD) 5 ml respectively, for the sensitization of biofilms. All photosensitizers (PS) were activated by LED at different wavelength. CHX was prepared in sterile distilled water and applied for 60 s. Each contaminated specimen was sprayed on all its surfaces with the aforementioned photosensitizers and control CHX. One-way analysis of variance (ANOVA) model was used to test the effect of the treatments and Tukey multiple comparison tests to compare means OF CFU/mL (log10) for exposed E. coli, C. albicans, S aureus, and S. mutans RESULTS: Specimens treated with 0.12% CHX (control) demonstrated a significant reduction in CFU/mL (log10) for exposed E. coli; 2.04±0.07 CFU/mL, C. albicans; 2.09±0.85 CFU/mL, S aureus; 3.04±0.11 CFU/mL, and S. mutans; 2.54±0.91 CFU/mL. The intragroup comparison revealed E.coli did not exhibit a decrease in reduction CFU/mL (log10) when acrylic resin irradiated with RB 5 µm. Whereas, CFU/mL (log10) values of S.aureus; 3.62±0.68 and S.mutans; 3.41±0.13 plummeted (p<0.05). Intergroup comparison showed E.coli values to display comparable reduction when disinfected with MB 500 mg/L and 0.12% CHX; 3.16±0.34 and 2.04±0.07 CFU/mL (log10) (p<0.05). CONCLUSION: Photosensitizers (RB, MB, PD) are selective in reducing bacterial count on acrylic resin blocks. CHX was found to be effective against all bacteria E.coli, C.albicans, S.aureus, and S.mutans at a concentration of 0.12%.


Assuntos
Fotoquimioterapia , Porfirinas , Resinas Acrílicas , Biofilmes , Dentaduras , Desinfecção , Escherichia coli , Azul de Metileno/farmacologia , Fotoquimioterapia/métodos , Fármacos Fotossensibilizantes/farmacologia , Polímeros , Rosa Bengala/farmacologia , Staphylococcus aureus , Streptococcus mutans
7.
Wiad Lek ; 74(6): 1433-1438, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34159933

RESUMO

OBJECTIVE: The aim: The work is devoted to studying the results of treatment of edentulous patients with dentures, made of thermoplastic material "Vertex ThermoSense". PATIENTS AND METHODS: Materials and methods: The non-acrylic thermoplastic plastic "Vertex ThermoSense" was used in our research work. The quality evaluation was carried out with the help of an objective-subjective test "BOFSAS", determination of biopotentials of masticatory muscles with the help of electromyography and determination of masticatory efficiency according to I.S. Rubinov. RESULTS: Results: It can be noted that the use of the basic thermoplastic material "Vertex ThermoSense" allows to achieve better fixation and stabilization of complete removable prostheses. It is subjectively confirmed by the "BOFSAS" test, objectively - by data of electromyographic studies and time the masticatory test of I.S. Rubinov. CONCLUSION: Conclusion: We can conclude that adaptation to dentures, made of the basic thermoplastic material "Vertex ThermoSense", goes in short terms and it is almost painless, based on the results of our studies.


Assuntos
Boca Edêntula , Adaptação Fisiológica , Dentaduras , Eletromiografia , Humanos
8.
J Prosthodont ; 30(S2): 143-149, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33988279

RESUMO

The development and expanded use of digital dentures has led to their use in the immediate loading of complete arch implant prostheses. This paper reviews the prosthetic complications that can occur with immediately loaded conversion prostheses along with the advantages afforded by digital dentures in minimizing or eliminating these complications. The process of using digital dentures in the fabrication of conversion dentures for immediate loading of complete arch implant prostheses is presented as well as the use of digital dentures for definitive prostheses. The described procedures include methods of obtaining clinical records, the fabrication of conversion dentures, the immediate loading procedure using a conversion prosthesis, and use of a conversion denture in the fabrication of a definitive prosthesis.


Assuntos
Implantes Dentários , Carga Imediata em Implante Dentário , Prótese Dentária Fixada por Implante , Dentaduras , Resultado do Tratamento
9.
J Adhes Dent ; 23(2): 145-158, 2021 Apr 07.
Artigo em Inglês | MEDLINE | ID: mdl-33825428

RESUMO

PURPOSE: To investigate the impact of pretreatment and conditioning on shear bond strength (SBS), surface free energy (SFE) and surface roughness (SR) between polyetheretherketone (PEEK) and cold-cured polymethylmethacrylate (PMMA). MATERIALS AND METHODS: PEEK substrates (Dentokeep PEEK Disc, nt-trading) were air abraded with Al2O3 particles of different grain sizes applied with varying pressure at 1) 0.2 MPa - 50 µm Al2O3; 2) 0.4 MPa - 50 µm Al2O3; 3) 0.2 MPa - 110 µm Al2O3; 4) 0.4 MPa - 110 µm Al2O3; or 5) without air abrasion (n = 172/group). Surface properties were quantified using SFE and SR (n = 10/group), and scanning electron microscope imaging (n = 2/group). Substrates were conditioned with a) Visio.link (VL, Bredent); b) Scotchbond Universal (SU, 3M Oral Care); c) Bonding Fluid (BF, Schütz Dental); or d) without conditioning (WC; n = 40/subgroup) and bonded to the polymer (Futura Jet, Schütz Dental). SBS and fracture types were determined before and after 10,000 thermal cycles (n = 20/subgroup). Univariate ANOVA, Kruskal-Wallis test, Mann-Whitney U-test, Kaplan-Meier survival estimates, and Weibull distribution were computed (p < 0.05). Ciba-Geigy tables and the chi-squared test were used to analyze fracture type distributions. RESULTS: An increase in particle size and pressure resulted in similar or increased SBS, Weibull characteristic strength, and Weibull moduli (p < 0.001 - 0.046). The lowest results were observed for the control group (without air abrasion), while pretreatment with 0.4 MPa - 110 µm Al2O3 presented the highest values (p < 0.001). In comparison with the other conditioning procedures, VL showed high (p < 0.001 - 0.03), and SU and WC low SBS (p < 0.001 - 0.006). Although it did not influence SFE, an increase in particle size and pressure led to an increased SR (p < 0.001). CONCLUSION: Pretreatment with 0.4 MPa - 110 µm Al2O3 can be recommended to increase bonding properties between PEEK and PMMA. Application of adhesives such as VL can enhance SBS further.


Assuntos
Colagem Dentária , Polimetil Metacrilato , Abrasão Dental por Ar , Análise do Estresse Dentário , Dentaduras , Cetonas , Teste de Materiais , Polietilenoglicóis , Cimentos de Resina , Resistência ao Cisalhamento , Propriedades de Superfície
10.
Arch Gerontol Geriatr ; 95: 104412, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33823472

RESUMO

BACKGROUND: Evidence suggests that denture non-use is significantly associated with activities of daily living, oral function, and cognitive decline. Few studies have focused on the possibility that the indications for denture use may differ depending on the cognitive and physical functions in older adults requiring long-term care. OBJECTIVES: The aim of this study was to elucidate the factors associated with denture non-use in older adults requiring long-term care. METHODS: This cross-sectional study comprised 201 older adults (45 men and 156 women; average age = 86.2 ± 7.1 years) requiring long-term care in Japan. Those who did not require denture treatment were classified by dentists into denture-use and denture non-use groups. The severity of dementia was assessed using the Clinical Dementia Rating scale. Multiple logistic regression analyses were conducted to detect the factors significantly associated with denture non-use. RESULTS: The proportion of participants without dentures was 58.2%. Multiple logistic regression analyses revealed that a moderate dementia (odds ratio [OR], 4.44; 95% confidence interval [CI] 1.18-16.71, p=0.027) and rinsing ability (OR 3.00; 95% CI 1.12-8.06; p=0.030) were significant factors related to the non-use of dentures. CONCLUSION: Severity of dementia and rinsing ability were significantly associated with non-use of dentures. These findings indicate the necessity of evaluating oral and cognitive functions while planning denture treatment in older adults requiring long-term care.


Assuntos
Atividades Cotidianas , Assistência de Longa Duração , Idoso , Idoso de 80 Anos ou mais , Estudos Transversais , Dentaduras , Feminino , Humanos , Japão , Masculino , Saúde Bucal
11.
Dent Mater J ; 40(4): 1055-1062, 2021 Jul 31.
Artigo em Inglês | MEDLINE | ID: mdl-33883326

RESUMO

The effect of the addition of nystatin and an alternative antifungal derived from pyrazoles in different commercial denture adhesives on their retentive efficacy, cytotoxicity and antimicrobial activity against Candida albicans was evaluated. Commercial denture adhesives were prepared with the inclusion of nystatin and 3,5-diaryl-4,5-dihydro-1H-pyrazole-1-carboximidamide (pyrazole) in three concentrations: 23.78 %w/w, 3.02 %w/w, and 0.31 %w/w (0.015 g, 0.0015 g, and 0.00015 g, respectively). The retentive efficacy was tested observing the influence of the medium, type of commercial denture type and the test condition (dipping). The antifungal action through disk diffusion and direct contact tests at 1, 4, 8 and 12 h and cytotoxic activity was evaluated in mouse fibroblasts (NIH/3T3) by the MTT reduction colorimetric assay. The addition of pyrazole and nystatin in commercial denture adhesives did not affect retentive efficacy rates and enhanced antifungal actions against Candida albicans. Results show a possibility of using denture adhesives as a delivery system for commercial antifungals (Nystatin) or pyrazole, with the second concentration (1,560 µg-3.02 %w/w) as the most efficient.


Assuntos
Antifúngicos , Candida albicans , Animais , Antibacterianos , Antifúngicos/farmacologia , Dentaduras , Camundongos , Testes de Sensibilidade Microbiana
12.
Ann Palliat Med ; 10(3): 3267-3276, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33849111

RESUMO

BACKGROUND: Dentition defect is a common symptom in clinical dental patients. This study compared the clinical effects of denture restoration and dental implant restoration in the treatment of dentition defects through meta-analysis. METHODS: Data retrieval was conducted through the PubMed, Web of Science, Embase, CNKI, and Wanfang databases. A total of 479 related literatures published in English or Chinese from 2013 to 2020 were included. Literature screening, data extraction and comprehensive evaluation, and analysis by meta-analysis was performed by 3 authors. RESULTS: A total of 17 studies and 1,459 patients were included. Among the 17 studies, the effective rate of treatment between the two groups was compared and the experimental group rate was significantly higher than that of the control group [odds ratio (OR) =6.149, 95% confidence interval (CI): 4.103-9.215, P<0.001]; the mastication function score was compared, and was higher in the experimental group than in the control group [standardized mean difference (SMD) =1.632, 95% CI: 1.039-2.224, P<0.001]; the retention function score was compared, and was higher in the experimental group than in the control group (SMD =1.775, 95% CI: 1.095-2.455), P<0.001); the aesthetics score was also compared, and was higher in the experimental group than in the control group (SMD =1.300, 95% CI: 0.499-2.100, P=0.001). Among 17 studies, 15 compared the comfort score, which was higher in the experimental group than in the control group (SMD =1.357, 95% CI: 0.455-2.258, P=0.003). CONCLUSIONS: Compared with denture restoration, dental implant restoration is more effective in the treatment of dentition defect with a higher comprehensive score of functional restoration.


Assuntos
Implantes Dentários , Dentição , Dentaduras , Humanos
13.
Artigo em Inglês | MEDLINE | ID: mdl-33770423

RESUMO

One of the most difficult aspects of providing removable dentures, for both the patient and the dentist, is the tooth selection process. The aim of this study was to assess the effectiveness of augmented reality (AR) on patient experience of the tooth selection process. MATERIALS AND METHODS: 3D scanning of upper anterior teeth of various shapes and sizes allowed for the creation of a virtual library that were subsequently used to programme an AR software application, 'ToothPick'. Seventeen participants were recruited to a comparative study at Leeds Dental Institute, over two clinical appointments, to trial the app and complete a 10-question survey to assess the efficacy of AR simulation. The sample size response generated outcome variables for 'perceived usefulness' and 'perceived ease of use' from the perspectives of the patient, dentist and technician. RESULTS: By the second appointment, 86% of participants preferred the app over the conventional methods for selection of denture teeth due to increased ease of use and accuracy that AR simulation had in replicating the wax try-in. CONCLUSION: The sample population favoured the adoption of AR technology to select denture teeth from the perspective of both the patient and the clinician.


Assuntos
Realidade Aumentada , Dente , Prótese Total , Dentaduras , Humanos , Software
14.
Proc Inst Mech Eng H ; 235(7): 751-761, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-33757320

RESUMO

Six-axis motion is essential for the evaluation of the wear failure modes of dental prostheses with complete teeth morphologies, and a high occlusal force capacity is vital for static clenching and dynamic bruxism. Additionally, the saliva environment influences abrasive particles and crack growth. The present research was aimed at the development of a six-axis masticatory and saliva simulator with these capacities. The masticatory simulator was designed based on a six-axis parallel mechanism, and the saliva simulator consisted of a saliva circuit and a temperature control loop. A control system of the masticatory and saliva simulators was constructed. The operating interface includes a centric occlusal position search, a static test, a dynamic test, a saliva supply, and data reporting. The motion and force performances of the masticatory simulator were evaluated. The flow rate and temperature change of the saliva simulator were calculated. For the occlusal position-searching, the driving amplitude is linear with the moving variables during minor one-axis motion. For the static tests, the force capacity of the driving chain is 3540 N, while for the dynamic tests, the force capacity is 1390 N. The flow rate of the saliva is 0.18-51.84 mL/min, and the saliva can effectively wet the prosthesis without the risk of overflow. Moreover, the saliva temperature can increase from room temperature (23°C) to body temperature (37°C) in about 6 min. The proposed DUT-2 simulator with six-axis motion, high force, and a salvia environment provides an in vitro testing approach to validate numerical simulation results and explain the clinical failure modes of prostheses. The centric occlusal position-searching, static tests, and dynamic tests could therefore be executed using a single testing machine. Moreover, the proposed device is more compact than previously reported six-axis masticatory simulators, including the Bristol simulator and DUT-1 simulator.


Assuntos
Força de Mordida , Procedimentos Cirúrgicos Robóticos , Dentaduras , Humanos , Mastigação , Saliva
16.
Artigo em Inglês | MEDLINE | ID: mdl-33669019

RESUMO

BACKGROUND: Dental health is an important factor in daily life routines and is closely associated with maintaining a health-related quality of life. This study examined denture procedure changes after implementation of the National Health Insurance (NHI) Coverage of Dentures for the elderly. METHODS: We used the "Korean Community Health Survey (KCHS)" developed by the Korea Centers for Diseases Control and Prevention. We analyzed the association between policy implementation and dental health-related outcomes using difference-in-differences (DID) analysis to compare patients aged ≥75 with those 65-74 years before and after coverage. RESULTS: A comparison of age groups and coverage periods showed that patients aged ≥75 years had higher (OR: 1.038, 95% CI: 1.021-1.055) procedure rates after coverage. In particular, elderly patients on medical aid had significantly higher denture procedure rates, while those without oral health screening were more likely to have denture procedures. CONCLUSIONS: This study determined the impact of the NHI Coverage of Denture procedure policy for the elderly and found increased denture treatments in the elderly. This policy appeared to positively affect older patients by increasing denture procedures for low-income and medical aid beneficiaries. Hence, the government needs to increase oral health examination and dental health policies for the elderly.


Assuntos
Programas Nacionais de Saúde , Qualidade de Vida , Idoso , Dentaduras , Inquéritos Epidemiológicos , Humanos , Cobertura do Seguro , República da Coreia
17.
J Oral Biosci ; 63(2): 175-183, 2021 06.
Artigo em Inglês | MEDLINE | ID: mdl-33662564

RESUMO

OBJECTIVES: The opportunistic fungus Candida albicans is a component of denture plaque and is associated with denture-related stomatitis. Inter-kingdom interactions between C. albicans and bacteria exist in such multi-species biofilms, which may affect the microbial composition of the plaque. This study was performed to investigate the bacterial composition of denture plaques, and the correlation between the relative abundance of these bacteria and C. albicans. METHODS: Thirty denture plaque and 16 dental plaque samples were collected from 18 denture wearers (mean age, 80.3 years). After DNA extraction, a meta 16S rDNA amplicon library was constructed using PCR primers targeting the V3-V4 hypervariable region of bacteria. The amplicon was evaluated by high-throughput sequencing, followed by bacterial population analysis. The concentrations of both C. albicans DNA and total bacterial DNA were determined by real-time PCR. The correlation between the relative abundance of each bacterial genus and C. albicans was analyzed through Spearman's rank correlation. RESULTS: The genera Streptococcus, Lactobacillus, Rothia, and Corynebacterium were found to be more abundant in dentures than in dental plaques. The predominant bacteria in healthcare-associated pneumonia also inhabited denture surfaces. C. albicans was positively correlated with three acidogenic bacteria and negatively correlated with Leptotrichia and pathogens associated with periodontitis and endocarditis. CONCLUSIONS: Dentures may be significant reservoirs of pathogens causing aspiration pneumonia. Bacteria showing negative correlation with C. albicans, such as Leptotrichia, may be useful for controlling the growth of C. albicans in antifungal therapies.


Assuntos
Placa Dentária , Microbiota , Idoso de 80 Anos ou mais , Bactérias/genética , Candida albicans , Dentaduras , Humanos , Microbiota/genética
18.
Int J Prosthodont ; 34(3): 291­299, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33616555

RESUMO

PURPOSE: To evaluate the effects of 0.2% sodium hypochlorite, Efferdent (Prestige Consumer Healthcare), and 6.25% Ricinus communis on biofilm removal and antimicrobial action on dentures and brushes using nonimmersion or immersion protocols for the brushes. MATERIALS AND METHODS: A total of 45 denture wearers were randomly assigned to a denture immersion protocol for 7 days: 0.85% saline solution for 20 minutes (control); 0.2% sodium hypochlorite for 20 minutes (SH); Efferdent for 3 minutes; or 6.25% Ricinus communis for 20 minutes (RC). The participants were also randomized to immersion (n = 23) or no immersion (n = 22) of their brushes with their dentures in the same solutions. For biofilm evaluation, the dentures were stained and photographed, and the area of the biofilm was measured using Image Tool 3.0 (University of Texas Health Science Center). To evaluate microbial load on dentures and brushes, the biofilm was collected, and the Candida spp and Streptococcus mutans colonies were counted. RESULTS: The SH, Efferdent, and RC groups showed reduced biofilm and Candida spp on dentures regardless of the immersion protocol for the brushes. However, no difference was found in the Candida spp counts collected from the brushes immersed compared to the brushes not immersed in the solutions. The SH and Efferdent groups showed reduced S mutans on both dentures and brushes, except for in the nonimmersion subgroups. CONCLUSION: All solutions reduced denture biofilm and microbial load. However, immersion of brushes in the solutions did not contribute to reducing the microbial load.


Assuntos
Biofilmes , Higienizadores de Dentadura , Contagem de Colônia Microbiana , Dentaduras , Humanos , Streptococcus mutans
19.
Int J Oral Maxillofac Surg ; 50(10): 1298-1302, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-33632577

RESUMO

The aim of this study was to investigate the role of lower dentures in the development of distally located stenoses of Wharton's duct and to further identify contributing factors to this mechanism. In a database of 352 patients with submandibular gland obstruction, three patients with four obstructed glands with stenosis of the ostium of Wharton's duct suspected to be caused by a lower denture were identified and further retrospectively analysed by studying medical records, operation reports, and clinical photographs. In all three cases, the causative lower dental prosthesis was implant-retained. All affected sublingual caruncles were in close relationship with the implants or the implant bar. Initially, all patients were advised to leave out the lower denture for a period of several weeks. One patient was free of symptoms after this period and did not develop any complaints after adjustment and replacement of the prosthesis. Surgical treatment with posterior rerouting of the orificium of Wharton's duct was performed in the remaining two patients because of persistent signs of obstruction. All patients were free of symptoms after long-term follow-up. Although not frequently occurring, implant-retained dental prostheses seem to play a role in the development of some distally located stenoses of Wharton's duct.


Assuntos
Implantes Dentários , Glândula Submandibular , Prótese Dentária Fixada por Implante , Dentaduras , Humanos , Estudos Retrospectivos , Ductos Salivares
20.
J Dent ; 106: 103589, 2021 03.
Artigo em Inglês | MEDLINE | ID: mdl-33524431

RESUMO

OBJECTIVES: Denture soft liner is applied to relieve pain from candida-induced denture stomatitis and promote healing, but with shortage of antifungal activity and easily harbors fungi. To overcome this problem, the in-situ method was used to synthesize silver nanoparticles (AgNPs) in acrylic soft liner to obtain antifungal effects. METHODS: Acrylic soft-liner with various weight percentage of silver 2-ethylhexanoate (0%, 0.1 %, 0.2 %, 0.3 %) were prepared in 10 mm × 10 mm × 3 mm. After chemical polymerization, the diameter of AgNPs synthesized in situ and the degree of conversion of each group were measured. After 3, 7, and 14 days of storage in water, the antifungal rate (AFR) of in vitro direct contact antifungal assays and the antifungal test of non-cumulative extract solution were measured respectively. The release profiles of silver ions from the specimen within 14 days were also evaluated. RESULTS: Evenly distributed AgNPs (4.7 nm-5.3 nm) were observed, and the degree of conversion had no significant difference among these groups. The AFR increased as the silver concentration rose, while decreasing with the storage time. After 14 days of water storage, the AFR of 0.2 % and 0.3 % groups still reached 63.38 % and 75.51 %, respectively. The non-cumulative extract solution had no antifungal effect. CONCLUSIONS: Within the service life, the acrylic soft liner containing AgNPs synthesized in situ had effective control of Candida albicans through direct contact. CLINICAL SIGNIFICANCE: This study suggests that AgNPs synthesized in situ may be an effective strategy in modifying acrylic denture soft liner to treat and prevent denture stomatitis.


Assuntos
Reembasadores de Dentadura , Nanopartículas Metálicas , Antifúngicos/farmacologia , Candida albicans , Dentaduras , Prata/farmacologia
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