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1.
J Indian Soc Pedod Prev Dent ; 38(3): 211-215, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33004717

RESUMO

Context: Delayed tooth eruption might be the primary or sole manifestation of local or systemic pathology. Aims: The aim of the study was to correlate Vitamin D level and eruption status of primary teeth. The objectives also included the assessment of the association between Vitamin D levels and socioeconomic status, infant's sun exposure, maternal sun exposure during pregnancy and religion. Settings and Design: A cross-sectional, observational study conducted on 96 infants aged 12-15 months. Materials and Methods: Ninety-six infants, aged 12-15 months were selected after obtaining parental consent. Blood samples were assessed for Vitamin D3 levels using the Vitamin D ELISA Kit. The eruption status of the teeth was recorded in all the 96 infants. The obtained data were subjected to the statistical analysis. Statistical Analysis Used: The obtained data were subjected to relevant statistical analysis such as Analysis of Variance, unpaired t-test, Kruskal-Wallis test, and Mann-Whitney U test, Chi-square Test, Tukey HSD, and Fisher's exact Test. Results: A significant correlation was found in the Vitamin D levels and the eruption timing (P < 0.001). The difference in mean Vitamin D levels among the three socio economic groups was not statistically significant (P = 0.088). A significant association was found between the infant's sun exposure and mother's sun exposure during pregnancy and religion on the Vitamin D levels (P = 0.002, P = 0.042, P = 0.002). Conclusions: Vitamin D deficiency can be considered as an etiological factor for delayed eruption. A strong association exists between the socioeconomic status, infant's sun exposure, maternal sun exposure during pregnancy, and religion with Vitamin D levels.


Assuntos
Erupção Dentária , Deficiência de Vitamina D , Estudos Transversais , Feminino , Humanos , Lactente , Gravidez , Dente Decíduo , Vitamina D
2.
J Indian Soc Pedod Prev Dent ; 38(3): 232-237, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33004720

RESUMO

Background: Dental trauma can determine the occurrence of sequelae in the deciduous tooth and due to the anatomical proximity to the germ of the successor permanent tooth, it frequently causes changes to the developing teeth. Aims: The objective of this study was to analyze clinically and radiographically traumatized primary teeth and permanent successors in children aged 0-8 years. Materials and Methods: Initially, a sample selection of medical records was made, designating the patients who fit the requirements; 247 patients were analyzed, totaling 379 traumatized primary teeth and 162 successive permanent teeth. Statistical Analysis: The results were developed using the Proportion Test and the Chi-square test at the 5% significance level. Results: Injuries to hard tissue prevailed (57%), with emphasis on coronary enamel fracture (49.1%). After clinical and radiographic examinations, 78% of traumatized primary teeth maintained pulpal vitality. At the clinical evaluation, the frequency of the developmental disorders observed in permanent successors was 10.5%, with enamel hypocalcification being the most common sequela. 17.3% of the clinical changes in the successor permanent teeth were caused by trauma to the supporting tissue, with the intrusive dislocation being responsible for the largest number of damages (37.5%). Conclusion: Based on the results found, it was concluded that the trauma occurring in the primary dentition were recorded and monitored more precisely and about the evaluated successive permanent teeth, except for the prevalence of sequelae found, the other factors are in agreement with the findings in the literature.


Assuntos
Hipoplasia do Esmalte Dentário , Avulsão Dentária , Criança , Pré-Escolar , Dentição Permanente , Humanos , Lactente , Recém-Nascido , Estudos Longitudinais , Dente Decíduo
3.
J Indian Soc Pedod Prev Dent ; 38(3): 304-310, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33004730

RESUMO

Background: Despite endodontic infections being a common problem in the primary dentition, some of the infected primary teeth can remain functional until the exfoliation through endodontic treatment. The primary endodontic treatment goal must be to optimize root canal disinfection and to prevent reinfection. Aim: The aim of this study is to compare the antifungal efficacy of antimicrobial photodynamic therapy, LASER, and 1% clotrimazole as irrigants by reducing the colony-forming units (CFU) of Candida albicans in the infected root canals of primary teeth. Materials and Methods: Pulp therapy was performed on deciduous mandibular second molars of 20 healthy children aged 5-8 years, which were divided into four groups of intracanal disinfection protocols: Group 1 (n = 5) - Sodium Hypochlorite, Group 2 (n = 5) - Photodynamic, Group 3 (n = 5) - Normal saline with LASER, and Group 4 - (n = 5) Antifungal. Disinfection potential was compared by collecting samples one just after access opening and the other after the experimental groups using paper points. The samples were cultured and incubated for 48 h to check change in CFU of the fungi. Results: Data were analyzed statistically using the Shapiro-Wilk's test, Mann-Whitney U-test; Kruskal-Wallis test, and Wilcoxon signed-ranks test. No significant difference (P > 0.05) was found between the different groups. However, complete inhibition of C. albicans CFU was seen with Group 4. Conclusion: One percent Clotrimazole (antifungal) can be utilized as an adjunct in the endodontic irrigation protocol for better success of pulpectomy in children.


Assuntos
Anti-Infecciosos , Fotoquimioterapia , Antifúngicos , Candida albicans , Criança , Pré-Escolar , Cavidade Pulpar , Desinfecção , Humanos , Projetos Piloto , Irrigantes do Canal Radicular/uso terapêutico , Preparo de Canal Radicular , Dente Decíduo
4.
Beijing Da Xue Xue Bao Yi Xue Ban ; 52(5): 907-912, 2020 Oct 18.
Artigo em Chinês | MEDLINE | ID: mdl-33047728

RESUMO

OBJECTIVE: To evaluate the clinical success of the treatment of maxillary anterior primary incisors caries with composite resin strip crowns. METHODS: Children who presented with severe early childhood caries and were treated comprehensive caries under general anesthesia from January to December 2016 were enrolled in this study. Composite crowns using preformed celluloid crown (3M ESPE, USA) called as "strip crown technique" were applied to treat vital primary incisors with two or multiple surface cavities. Selective etchings of enamel, Universal Bond adhesive and 3M Z350 resin were used to make strip crowns. The patients returned at the end of 6, 12 and 18 months and received clinical and radiologic examinations. Dentists who did not attend the treatment evaluated the strip crowns clinically by modified United States Public Health Service (USPHS) criteria. RESULTS: Four hundred eighteen restorations, placed in 127 children aged 1.17-5.75 years (average of 3.22), were evaluated. The overall retention rate was 97.8% at the end of 6 months, 93.6% at 12 months and 89.2% at 18 months. After 18 months, 28 restorations (6.7%) were totally lost and 4.1% were rated as having lost some resin material. Only four teeth (1.0%) had secondary caries at the end of 18 months and 1.4% teeth had pulpal pathology requiring root canal treatment. Composite crowns had good performance in contour and adjacent contact and improved aesthetics significantly. Twenty-nine teeth (6.9%) showed mild gingivitis and 93.1% showed healthy gingival. 11.2% of the teeth demonstrated color change because plaque accumulation and the polish could remove the stains. The complete loss of strip crowns was mainly related to eating bites. CONCLUSION: Strip crowns performed well for restoring primary incisors with large or multisurface caries for periods of over 18 months. The strip crowns can be a durable and aesthetic restoration for vital carious primary incisors with adequate tooth structure after caries removal. Functional movement is an important cause of complete loss. Because of the high technical sensitivity and its requirement of the cooperation of children, strip crowns are more suitable for older and cooperative children as well as children receiving dental treatment under sedation or general anesthesia.


Assuntos
Incisivo , Dente Decíduo , Criança , Pré-Escolar , Resinas Compostas , Coroas , Humanos , Lactente , Maxila , Estados Unidos
5.
Eur J Paediatr Dent ; 21(3): 235-237, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32893658

RESUMO

BACKGROUND: Segmental odontomaxillary dysplasia is an uncommon nonhereditary growth disorder that affects the maxilla, gums and ipsilateral dentition. The disorder is diagnosed mainly based on dental (over-retention of primary teeth, dental agenesis and diastemas) and bone findings (bone sclerosis, irregular trabeculation of immature bone and reduced maxillary sinus). This paper provides a case report. CASE REPORT: A 5-year-old child with skin manifestations including hypertrichosis, facial erythema and pigmented nevus was diagnosed with type II segmental odontomaxillary dysplasia based on clinical, radiographic and histopathological analysis. CONCLUSION: The skin findings can help with the suspicion of segmental odontomaxillary dysplasia, although the definitive diagnosis is typically established by a paediatric dentist based on clinical and radiological findings.


Assuntos
Diastema , Odontodisplasia , Dermatopatias , Pré-Escolar , Humanos , Maxila , Dente Decíduo
6.
Evid Based Dent ; 21(3): 100-101, 2020 09.
Artigo em Inglês | MEDLINE | ID: mdl-32978541

RESUMO

This paper reports the findings from a research project named FiCTION (Filling Children's Teeth: Indicated or Not?).Design FiCTION was a multi-centre, parallel-group, patient-randomised controlled trial comparing the effectiveness of three treatment strategies. There were three strategies within the trial: conventional caries removal and restoration plus prevention (C+P); biological sealing-in caries plus prevention (B+P); and prevention alone (PA).Case selection Children aged 3-7 years with at least one asymptomatic primary molar carious into dentine were included in the study. Participants were randomly allocated to one of the three treatment strategies. Dental professional's (DPs) attended one day training for treatment delivery. Seventy two practices randomised at least one participant. Ten practices withdrew but data collected to the withdrawal date were included in the analysis. The project ran for 3 years between 2012 and 2015. One thousand and fifty eight participants (C+P:352; B+P:352; PA:354) from 68 practices were included in the analysis set. Baseline demographic and clinical characteristics for each arm of the study were balanced.During routine practice visits data were collected on a case report form (CRF). The data included:A. Co-primary outcomes: pain due to caries and evidence of dental infection (without radiographic evidence)B. Secondary outcomes: cost effectiveness, oral health related quality of life (COHRQOL), parental and caregivers perception questionnaire (P-CPQ-16), modified child dental anxiety scale, child and parent-reported anticipatory and treatment-related anxiety, caries incidence at baseline and final visit, qualitative views of parents/carers and DPs.Sound methodology was described in the narrative of the paper. The median follow-up period was 33.8 months (IQR 23.8, 36.7).Results For the co-primary outcomes the proportions of participants with at least one episode of dental pain and/or infection were: C+P 42%, B+P 40%, PA 45%. When comparing C+P with B+P and PA, there were no significant observed differences, adjusted risk difference (97.5%CI): B+P vs C+P -2%(-10%, 6%), PA vs C+P 4% (-4%, 12%). The number of episodes of dental pain and/or infection were C+P 0.62 (sd 0.95), B+P 0.58 (sd 0.87), PA 0.72 (sd 0.98). When comparing C+P with B+P and PA no significant differences were observed, adjusted incident rate ratio (97.5% CI): B+P vs C+P 0.95 (0.75, 1.21), PA vs C+P 1.18 (0.94, 1.48).Secondary outcomes:• Cost effectiveness, PA was the least costly but least effective for both co-primary outcomes with little evidence in this paper as to how and why B+P and C+P would provide greater benefits• COHRQOL, P-CPQ-16, anxiety showed no statistical differences• Parent reported anticipatory anxiety showed differences between PA vs C+P; 6% lower in the PA arm, adjusted risk difference -0.06 ((97.5% CI): -0.11 to -0.003)• Caries incidence showed no differences in the caries in primary or first permanent molars• Qualitative views showed that all three approaches were generally acceptable but trust in the dental professional played a significant role. Local anaesthesia and dental extractions were generally viewed more negatively.Conclusions There were no statistical differences between the three strategies for the co-primary outcomes, namely the incidence or number of episodes of dental pain and/or infection experienced by the study participants. The secondary outcomes suggest less anticipatory anxiety in the PA arm of the trial and that local anaesthesia and dental extractions were viewed negatively.


Assuntos
Cárie Dentária , Medicina Geral , Criança , Pré-Escolar , Humanos , Dente Molar , Qualidade de Vida , Dente Decíduo
7.
J Appl Oral Sci ; 28: e20200259, 2020 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-32901695

RESUMO

Mineralization-promoting peptides are attractive candidates for new remineralization systems. In previous studies, peptides have been applied as aqueous solutions, which is not a clinically relevant form. OBJECTIVE: This study aims to investigate the efficiency of a mineralization-promoting peptide, applied in varnish, on remineralizing artificial caries on primary teeth. METHODOLOGY: 55 primary molars were collected. Specimens were immersed in a demineralizing solution for 7 days and then, divided into 7 groups: Baseline: No-remineralization, Placebo: Blank colophony, F: Colophony 5% fluoride, P: Colophony 10% peptide, P+F: Colophony 5% fluoride and 10% peptide, Embrace: Embrace™ varnish, Durashield: Durashield™ varnish. A mixture of 35% w/v colophony varnishes were prepared in ethanol and applied accordingly. Specimens were immersed in a remineralization solution for 4 weeks and it was evaluated using PLM and SEM. Lesion depth reduction was examined by one-way ANOVA. RESULTS: There was no significant difference in mean lesion depths between baseline (147.04 ± 10.18 µm) and placebo groups (139.73 ± 14.92 µm), between F (120.95 ± 12.23 µm) and Durashield (113.47 ± 14.36 µm) groups and between P (81.79 ± 23.15 µm) and Embrace (90.26 ± 17.72 µm) groups. Lesion depth for the P+F group (66.95±10.59 µm) was significantly higher compared to all other groups. All groups contained samples with subsurface demineralized regions. Number of subsurface demineralized regions were higher in fluoride-containing groups. CONCLUSIONS: We conclude that the mineralization-promoting peptide (MPP3) is effective in this in vitro study and the peptide shows benefits over fluoride as it yields less subsurface demineralized regions.


Assuntos
Cárie Dentária , Remineralização Dentária , Cariostáticos , Criança , Fluoretos Tópicos , Humanos , Pintura , Peptídeos , Dente Decíduo
8.
Oral Health Prev Dent ; 18(1): 741-746, 2020 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-32895657

RESUMO

PURPOSE: To verify the prevalence of developmental defects of enamel (DDE) in deciduous teeth and analyse the association with adverse events that occurred during pregnancy and early childhood. MATERIALS AND METHODS: In a cross-sectional study, 152 children with an average age of 3.57 ± 1.25 years were examined according to the criteria established by the DDE index. A previously validated questionnaire was given to mothers in order to obtain information regarding: calcium and vitamin D deficiency (measured in mothers); gestational diabetes; gestational undernutrition; weight at birth; neonatal hypoxia; and presence of asthma in early childhood. The clinical exam was conducted by a single examiner calibrated for visual exams (Kappa = 0.84), outdoors on patios of schools with children and examiner knee to knee. Pearson's chi-squared test and Fisher's Exact Test (p < 0.05) were used to determine statistically significant associations between the variables in study. The data were then analysed using a binary logistic regression regression. RESULTS: 26.3% of children exhibited DDE. It was possible to verify a statistically significant association between DDE and vitamin D deficiency (p < 0.01), calcium deficiency (p = 0.01), neonatal hypoxia (p = 0.026), and gestational diabetes (p = 0.04). The regression model allowed the conclusion that children who had neonatal hypoxia during childbirth, gestational diabetes or vitamin D deficiency during their gestation were 3.54, 12.47 and 6.40 more likely to exhibit signs of DDE, respectively. CONCLUSION: The prevalence of DDE was considered high and was associated with vitamin D and calcium deficiency during pregnancy, gestational diabetes, and neonatal hypoxia.


Assuntos
Hipoplasia do Esmalte Dentário , Criança , Pré-Escolar , Estudos Transversais , Esmalte Dentário , Feminino , Humanos , Mães , Gravidez , Prevalência , Dente Decíduo
9.
Zhonghua Kou Qiang Yi Xue Za Zhi ; 55(9): 639-646, 2020 Sep 09.
Artigo em Chinês | MEDLINE | ID: mdl-32878399

RESUMO

Objective: To compare the caries experience and the kinds of dental treatment between children with autism spectrum disorders (ASD) and children without systemic disease who were all treated under general anesthesia. Methods: Totally 103 children with ASD who received dental treatments under general anesthesia in 13 professional dental hospitals around China from April to November 2016 were included in the present study. A group of 97 children without systemic disease, according to the age, gender and application propensity score matching method, were chosen as controls, who received dental treatments under general anesthesia between January 2015 to November 2018 in the same hospitals as the children with ASD. Decay missing filling tooth (DMFT/dmft, DMFT for permanent teeth and dmft for primary teeth) indices of two groups of children and the contents of the dental treatments under general anesthesia were analyzed. Results: No significant difference of DMFT/dmft index ï¼»M (Q 25, Q 75)ï¼½ was found between children with ASD group ï¼»0 (0, 3)/11(8, 14)ï¼½ and control group ï¼»0 (0, 3)/9(7, 13)ï¼½ (P>0.05). The average number of dental treatments under general anesthesia and the average number of endodontic treatment in children with ASD were 13 (11, 15) and 3 (2, 6) teeth respectively, while those in the control group were 12 (9, 14) and 2 (1, 4) teeth respectively, the differences were statistically significant (P<0.01, P<0.05). Conclusions: No significant difference was found between children with ASD and the normal controls who receive dental treatments under general anesthesia in DMFT/dmft index, but the treatment needs of children with ASD is relatively higher, and their tooth decay is relatively severer.


Assuntos
Transtorno do Espectro Autista , Cárie Dentária , Anestesia Geral , Criança , China , Índice CPO , Assistência Odontológica , Humanos , Dente Decíduo
10.
Zhong Nan Da Xue Xue Bao Yi Xue Ban ; 45(6): 678-683, 2020 Jun 28.
Artigo em Inglês, Chinês | MEDLINE | ID: mdl-32879125

RESUMO

OBJECTIVES: To explore the difference in odontoblast differentiation capacity between stem cells from human exfoliated deciduous teeth (SHED) and dental pulp stem cells (DPSCs), and to examine the expression level of ephrinB1 in odontoblast differentiation of these stem cells. METHODS: The stems cells were divided into a SHED group and a DPSCs group. After odontoblast differentiation induction, the above 2 groups were also randomly divided into a 3 d group and a 7 d group, respectively.The calcium deposition was detected by alkaline phosphatase (ALP) staining and alizarin red staining.The mRNA and protein expressions of ephrinB1, dentin matrix protein-1 (DMP-1) and dentin sialophosphoprotein (DSPP) were detected by real-time PCR and Western blotting. RESULTS: ALP staining and alizarin red staining showed that there was stronger mineralization capacity in the SHED group than that in the DPSCs group. The relative mRNA and protein expressions of DMP-1, DSPP, and ephrinB1 in the SHED group were higher than those in the DPSCs group except for the protein expression of DMP-1 in the SHED 3 d group (all P<0.05). CONCLUSIONS: SHED has stronger odontoblast differentiation capacity than DPSCs. In addition, ephrinB1 may be involved in the processes of odontoblast differentiation in the SHED and DPSCs.


Assuntos
Odontoblastos , Osteogênese , Diferenciação Celular , Proliferação de Células , Células Cultivadas , Polpa Dentária , Humanos , Células-Tronco , Dente Decíduo
11.
Quintessence Int ; 51(10): 844-852, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32901239

RESUMO

OBJECTIVE: To evaluate the clinical performance of the fiberglass "Figaro" crowns compared to preformed metal crowns (PMCs) in pulpotomized primary molars in children. METHOD AND MATERIALS: This split-mouth controlled randomized clinical study was performed on ten childrens, aged from 4 to 6 years, who needed complete oral rehabilitation under general anesthesia and had one pair or two pairs of their primary molars requiring pulpotomy. After pulp therapy, the teeth were randomly assigned to Figaro or PMC crown groups. Modified United States Public Health Service (USPHS) criteria were used to evaluate crown retention, marginal integration, crown discoloration, secondary caries, and gingival status, at 3 and 6 months. The data were analyzed using the chi-square test with Monte Carlo (MC) correction and McNemar (McN) test at a significance level of .05. RESULTS: Ten patients (three boys, 30%; seven girls, 70%) with a mean age of 4.65 ± 0.709 years participated in this study. At 3 months follow-up, there was no significant difference between the groups, but at 6 months follow-up, Figaro crowns showed significant changes from intact crown to either chipped or large loss, whereas all PMCs were found intact (χ2 = 14.545, P[MC] = 0.000). There was also significant deterioration in Figaro crowns' color after 6 months (χ2[McN] = 8.1, P = .004). CONCLUSION: PMCs were more durable than Figaro crowns, which showed significant deterioration after the 6-month follow-up period.


Assuntos
Coroas , Dente Decíduo , Criança , Pré-Escolar , Feminino , Vidro , Humanos , Masculino , Metais , Dente Molar
12.
Braz Oral Res ; 34: e102, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32876119

RESUMO

The aim of this study was to evaluate and compare the clinical and radiographic success rates of pulpotomies in primary molars using a new stain-free tricalcium silicate cement (Bio-C Pulpo) versus a conventional white MTA (MTA Angelus) as a pulpotomy medicament for primary teeth. Seventy primary teeth in 33 patients were randomly divided into two groups, MTA Angelus (n = 34) and Bio-C Pulpo (n = 36). Clinical and radiographic follow-up examinations were conducted at seven days (except radiographic follow-up), and at one, three, six and 12 months. At 12 months, the clinical success rate of the MTA Angelus group was 100 percent (28 out of 28), and the radiographic success rate was 100 percent (28 out of 28). In comparison, the clinical success rate for the Bio-C Pulpo group was 100 percent (29 out of 29), and the radiographic success rate was 100 percent (29 out of 29). No significant differences were found between the groups at any follow-up evaluation. According to Fisher´s exact test, a significant difference was observed between the pulpotomy agent and tooth discoloration (p < 0.001). The success rates were 100% for the Bio-C Pulpo group, with no case of discoloration, and 10.31% for the MTA Angelus group. Both MTA Angelus and Bio-C Pulp pulpotomy medicaments are appropriate options for pulpotomies in primary teeth, and enable high clinical and radiographic success rates. However, more long-term studies are required to test the new Bio-C Pulpo medicament.


Assuntos
Pulpotomia , Compostos de Alumínio , Compostos de Cálcio , Cimentos Dentários , Combinação de Medicamentos , Humanos , Óxidos , Silicatos , Dente Decíduo , Resultado do Tratamento
13.
J Dent Child (Chic) ; 87(2): 60-68, 2020 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-32787998

RESUMO

Purpose: Although rapid maxillary expansion (RME), transpalatal arch (TPA) and cervical-pull headgear (HG) have been suggested for the interception of palatally displaced permanent canines (PDCs), existing knowledge remains inconclusive. The purpose of this study was to assess these practices in an evidence-based manner.
Methods: Randomized controlled trials (RCTs) investigating the prevalence of physiologic PDC eruption after using RME, TPA and HG, with or without the extraction of the primary canines, were searched, and the risk of bias was assessed.
Results: Data from five RCTs were included. RME, TPA and HG, with or without extracting primary canines, can significantly increase the rate of normal eruption of PDCs compared to no intervention (risk ratio [RR] = 2.5 to 4.5). In comparison to extraction, no difference was observed, except for HG combined with primary canine extraction (RR = 1.413; 95 percent confidence interval = 1.062 to 1.880).
Conclusion: RME, TPA and HG can significantly increase the rate of normal eruption of PDC compared to no intervention. However, when compared to extraction, no differences were noted, except for HG combined with primary canine extraction.


Assuntos
Erupção Ectópica de Dente , Dente Canino , Humanos , Maxila , Ortodontia Interceptora , Extração Dentária , Dente Decíduo
14.
J Dent Child (Chic) ; 87(2): 69-76, 2020 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-32787999

RESUMO

Purpose: To evaluate the morphological alterations in enamel and dentin of primary teeth following radiotherapy (RT) and to determine the best adhesive technique and time to carry out restorative procedures.
Methods: Enamel and dentin fragments of primary teeth were randomly assigned into four groups (n=30): G1 (control)-non-irradiated, only restorative procedure; G2- restorative procedure immediately before RT; G3-restorative procedure 24 hours after RT; and G4-restorative procedure six months after RT. Each group was divided into one of two subgroups according to the adhesive system used for restoration: (1) AdperSingle Bond 2 (SB); and (2) ClearfillSE Bond (CL). The specimens were submitted to fractionated RT until they reached the final dose of 60 Gy. They were then subjected to confocal microscopy and the shear bond strength test. Data were analyzed using two-way analysis of variance followed by Tukey's tests ( α = five percent).
Results: Morphological changes were first observed in enamel and dentin after 40 Gy of irradiation. G4 bond strength values were similar to G1 in the CL and SB groups for enamel and in the CL group for dentin (P >0.05). G2 showed the lowest values for enamel and dentin (P <0.05). In G3, CL presented the highest strength values in enamel; for G4, the highest values were found in dentin (P <0.05).
Conclusions: Radiotherapy affected the morphological surface of enamel and dentin. The restorations placed immediately after RT had the weakest shear bond strength, and the restorations placed six months after RT had similar means of bond strength compared to the nonirradiated teeth in enamel, regardless of the adhesive system used. In dentin, CL showed better performance than SB.


Assuntos
Colagem Dentária , Adesivos Dentinários , Resinas Compostas , Cimentos Dentários , Dentina , Humanos , Teste de Materiais , Cimentos de Resina , Resistência ao Cisalhamento , Dente Decíduo
15.
J Dent Child (Chic) ; 87(2): 83-89, 2020 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-32788001

RESUMO

Purpose: Radiation-related caries is characterized by enamel delamination near the dentinoenamel junction (DEJ). We investigated the activity and expression of the matrix metalloproteinases (MMPs) -2 and -9 in order to understand disease pathogenesis in teeth submitted or not to radiotherapy (RT).
Methods: In situ zymography and immunofluorescence assays were performed to evaluate the activity and expression of MMPs -2 and -9, respectively. Twelve primary second molars were randomly assigned into two experimental subgroups: irradiated and nonirradiated. Dental fragments were exposed to radiation at a dose fraction of two Gy for five consecutive days until reaching the total dose of 60 Gy. The percentage of fluorescence in the DEJ was evaluated in three distinct regions of the tooth (cervical, cusp, and pit). The regions were photographed under fluorescence microscopy at 1.25× and 5× magnification.
Results: The intensity of fluorescence per mm 2 in the DEJ was higher in the cervical region of irradiated primary teeth (P <0.05) versus nonirradiated ones. In these areas, immunofluorescence revealed expression of MMPs -2 and -9.
Conclusion: Radiotherapy can increase the activity of MMPs -2 and -9 in the cervical region of the DEJ of primary teeth.


Assuntos
Dentina , Metaloproteinases da Matriz , Esmalte Dentário , Humanos , Dente Molar , Dente Decíduo
16.
Dental Press J Orthod ; 25(3): 85-92, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32844968

RESUMO

INTRODUCTION: Stem cells obtained from the pulp of human deciduous teeth are highly proliferative and plastic multipotent cells, which makes them a relevant model of stem cells, applied in several biomedical areas, with different purposes. OBJECTIVE: Based on a brief review of the literature, the present work intends to present from conceptual aspects about stem cells, classifications, potential (in vitro and in vivo) applications in dental practice, cell culture, cryopreservation and its importance, ethical and regulatory aspects, as well as the role of the dental surgeon as the endorser responsible for the entire clinical stage that involves the process of collecting stem cells obtained from dental pulps for cryopreservation, with a view to using them under appropriate conditions, in accordance with scientifically proven and justified good laboratory and clinical practices.


Assuntos
Células-Tronco Mesenquimais , Adulto , Polpa Dentária , Odontologia , Humanos , Células-Tronco , Dente Decíduo
17.
Pediatr Dent ; 42(4): 256-461, 2020 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-32847665

RESUMO

Purpose: The purpose of this systematic review and meta-analysis was to assess success rates for nonvital treatment in primary teeth for caries/trauma. Methods: Databases were searched between 1960 and 2020 for randomized controlled trials, cohorts, case series, and in vitro studies. The primary outcome was overall success (clinical and radiographic) for pulpectomy and lesion sterilization tissue repair (LSTR). Included articles were independently determined, agreed upon, data extraction assessed, risk of bias, meta-analyses, and assignment of quality of evidence (GRADE). Results: Comparing teeth with and without root resorption, pulpectomy success was better (P<0.001) in teeth without preoperative root resorption. Success with pulpectomies performed with zinc oxide eugenol [ZOE] and with Endoflas (ZOE plus iodoform plus calcium hydroxide) did not differ from that observed using Vitapex or Metapex (iodoform plus calcium hydroxide; P≥0.50) after 18 months; however, Endoflas and ZOE success rates remained near 90 percent versus 71 percent or less for iodoform. Network analysis ratings showed Endoflas and ZOE performed better than iodoform alone. Also, LSTR performed better (P<0.001) than pulpectomies in teeth with preoperative root resorption, but pulpectomy results were superior (P=0.09) if roots were intact. Rotary instrumentation of root canals was significantly faster (P<0.001) than manual instrumentation. Success rates were not impacted by method of obturation or root length determination, type of tooth, number of visits, irrigants, smear layer removal, or timing/type of final restoration. Conclusions: Eighteen-month success rates support Endloflas and zinc oxide eugenol pulpectomies over iodoform pulpectomies. Lesion sterilization tissue repair had limited indication for teeth with resorbed roots.


Assuntos
Cárie Dentária , Materiais Restauradores do Canal Radicular , Reabsorção da Raiz , Hidróxido de Cálcio , Humanos , Pulpectomia , Dente Decíduo , Cimento de Óxido de Zinco e Eugenol
18.
Pediatr Dent ; 42(4): 288-292, 2020 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-32847668

RESUMO

Purpose: The purpose of this study was to examine if there are differences in the success rate of primary teeth treated with pulpotomies performed by general dentists (GDs) versus pediatric dentists (PDs), using submitted paid insurance dental claims. The extraction time after pulpotomy was examined. Methods: Data for this study were obtained from a private dental insurance claims warehouse. The insurance claims data were retrieved, and a retrospective analysis was performed over seven years (2008 to 2015). The survival rate was estimated using proportional hazard survival analysis. Results: The total number of paid dental claims that used the CDT code for pulpotomy was 401,638. For teeth that received pulpotomy, the average time between pulpotomy and extraction was 1.1 years if the pulpotomies were performed by GDs. The average time was 1.3 years if the pulpotomies were performed by PDs (P<0.0001). GDs had an extraction rate of 7.83 percent after pulpotomies and the placement of stainless steel crowns, while PDs had an extraction rate of 4.09 percent. Conclusions: There is a significant difference in the success rate of teeth treated with pulpotomies performed by pediatric dentists versus general dentists, with pulpotomies performed by the former having a longer survival rate.


Assuntos
Análise de Dados , Pulpotomia , Criança , Coroas , Odontólogos , Humanos , Dente Molar , Estudos Retrospectivos , Dente Decíduo , Resultado do Tratamento
19.
Pediatr Dent ; 42(4): 293-299, 2020 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-32847669

RESUMO

Purpose: The purpose of this study was to evaluate the clinical and radiographic effectiveness of Biodentine® as a pulpotomy medicament for use in primary molars. Methods: A retrospective chart review was conducted on children who had received full-mouth dental rehabilitation under general anesthesia between January 1, 2013, and May 1, 2018. Three clinical outcomes (pain, pathologic mobility, and soft tissue pathology) and two radiographic outcomes (pathologic radiolucency and pathologic root resorption) defined a composite outcome of pulp therapy failure. Results: The final study sample consisted of 208 children with a mean age of 4.9 years. The total number of primary molars that received a Biodentine® pulpotomy over the 30-month posttreatment period was 608. Survival analyses determined that the cumulative probability of clinical survival at 30 months was 93.7 percent (95 percent confidence interval [95% CI] equals 83.7 percent to 99.2 percent) and radiographic survival probability was 85.6 percent (95% CI equals 76.3 percent to 93.7 percent). The majority (n equals 14 out of 20; 70 percent) of failed Biodentine® pulpotomies occurred before 18 months. Conclusions: Primary molar pulpotomies utilizing Biodentine® as the pulpal medicament had favorable clinical and radiographic results after 30 months. Biodentine® can be considered an alternative to other commonly used primary tooth pulpotomy medicaments.


Assuntos
Compostos de Cálcio , Pulpotomia , Criança , Pré-Escolar , Combinação de Medicamentos , Humanos , Dente Molar , Óxidos , Estudos Retrospectivos , Silicatos , Dente Decíduo , Resultado do Tratamento
20.
Med Oral Patol Oral Cir Bucal ; 25(5): e668-e674, 2020 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-32851984

RESUMO

BACKGROUND: The objective of this study was to describe the bacterial communities associated with pediatric patients with endodontic infections of temporal teeth by targeting the 16S rRNA gene using pyrosequencing. MATERIAL AND METHODS: Microbiological samples were obtained from the lower primary molars of thirteen 13 pediatric patients with dental infections. An aspiration method for microbiological sampling was used. The identification of microbiota employing the pyrosequencing method by targeting the 16S gene was performed. RESULTS: Ribosomal 16S RNA gene sequences were amplified, obtaining a total of 16,182 sequences from 13 primary infected molars (13 different individuals) by pyrosequencing. Bacteroidetes phyla (35.15%) were the most abundant followed by Firmicutes (33.3%) and Fusobacteria (10.05%); the presence of specific pathogenic bacteria was determined as well. CONCLUSIONS: The infected root canal of primary teeth contains a high diversity of anaerobic bacteria, and Bacteroidetes, Firmicutes, and Fusobacteria phyla were the most abundant; Prevotella and Streptococcus genera were the most prevalent.


Assuntos
Bactérias , Bacteroidetes/genética , Criança , Humanos , RNA Ribossômico 16S , Análise de Sequência de DNA , Dente Decíduo
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