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1.
Trials ; 21(1): 140, 2020 Feb 04.
Artigo em Inglês | MEDLINE | ID: mdl-32019596

RESUMO

BACKGROUND: Silver diamine fluoride (SDF) solution is effective in arresting early childhood caries (ECC). Previous studies have suggested that it might exert a preventive effect in managing ECC. However, no well-designed clinical trials have yet been performed to study the effect of SDF on caries prevention. The objective of this randomised clinical trial is to determine whether 38% SDF solution is superior to 5% sodium fluoride (NaF) varnish in preventing new carious lesions in primary anterior teeth. METHODS/DESIGN: This is a phase II, single-centre, randomised, double-blind, active-controlled, parallel-group pragmatic trial. The hypothesis tested is that 38% SDF would be more effective than 5% NaF in preventing new caries development in primary anterior teeth. Approximately 730 3-year-old children who are generally healthy and with parental consent will be recruited from Hong Kong kindergartens. This sample size will be sufficient for appropriate statistical analysis of a superiority trial with 90% power, allowing for a 20% drop-out rate. Stratified randomisation will be adopted for allocating the intervention. The intervention will either be 38% SDF or 5% NaF (as a positive control) therapy on primary upper anterior teeth. A single trained examiner will conduct a dental examination every 6 months until 30 months in kindergarten. Another operator will provide fluoride therapy immediately after each dental examination. The examiner, children and children's parents will be blinded to the treatment allocation. A questionnaire survey will be conducted to study the children's oral health-related behaviours and socioeconomic backgrounds. Chi-square tests, t tests, regression analyses and survival analyses will be adopted for data analysis. DISCUSSION: The effectiveness of 38% SDF in preventing ECC remains uncertain. If the results are as anticipated, care standards using 5% NaF for ECC prevention will be changed. In addition, the results will be widely available and increase the adoption of SDF in other countries to reduce the global burden of ECC. TRIAL REGISTRATION: ClinicalTrials.gov, NCT04075474. Registered on 30 Aug 2019.


Assuntos
Cariostáticos/uso terapêutico , Ensaios Clínicos Fase II como Assunto , Cárie Dentária/prevenção & controle , Compostos de Amônio Quaternário/uso terapêutico , Compostos de Prata/uso terapêutico , Fluoreto de Sódio/uso terapêutico , Cariostáticos/farmacologia , Pré-Escolar , Método Duplo-Cego , Feminino , Fluoretos Tópicos/farmacologia , Fluoretos Tópicos/uso terapêutico , Seguimentos , Hong Kong , Humanos , Masculino , Ensaios Clínicos Pragmáticos como Assunto , Compostos de Amônio Quaternário/farmacologia , Compostos de Prata/farmacologia , Fluoreto de Sódio/farmacologia , Dente Decíduo/efeitos dos fármacos , Resultado do Tratamento
2.
J Dent ; 88: 103165, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-31279925

RESUMO

OBJECTIVES: This clinical trial investigated the efficacy of silver diamine fluoride (SDF) in arresting dentine caries in primary molars of preschoolers. Time required for treatment, adverse effects, parental aesthetic perception, anxiety and oral health related to quality of life (OHRQoL) was evaluated. MATERIALS AND METHODS: Children, 2-5 years old, with active dentine caries lesions on the occlusal surface of primary molars were randomly allocated to test group (SDF) or control group (atraumatic restorative treatment/ART). The dmf-t/DMF-T and ICDAS indexes determined the presence of caries and activity. The main outcome after 3, 6 and 12-month follow-up was assessed by a blind examiner. The time required to perform the treatments was recorded and a facial image scale was applied to assess anxiety before and after treatment. Adverse events and aesthetic perception were assessed through questions addressed to caregivers; and the OHRQoL through the B-ECOHIS questionnaire. RESULTS: In 68 patients that were randomized, the mean number of treated teeth per child was 2.42(1.04) and 2.09(1.18) in the SDF and ART groups (p = 0.074), respectively. The mean difference of arrested lesions between the groups after 12 months was -0,07(0.05; - 0.17-0.30). The time required to treat with SDF was lower than the ART (p < 0.001). There was no difference in the percentage of adverse events + aesthetic perception (p = 0.709), and the change in anxiety (p = 0.155). There was a less impact in OHRQoL after ART treatment, but only when the parents' distress subscale was considered (p = 0.012). CONCLUSION: SDF requires much less chair-time and have similar results as ART in arresting caries lesion, anxiety, adverse effects, aesthetic perception and quality of life.


Assuntos
Cariostáticos/uso terapêutico , Tratamento Dentário Restaurador sem Trauma/métodos , Cárie Dentária/tratamento farmacológico , Fluoretos Tópicos/uso terapêutico , Dente Molar/efeitos dos fármacos , Compostos de Amônio Quaternário/uso terapêutico , Compostos de Prata/uso terapêutico , Dente Decíduo/efeitos dos fármacos , Pré-Escolar , Tratamento Dentário Restaurador sem Trauma/psicologia , Dentina , Feminino , Humanos , Masculino , Qualidade de Vida , Resultado do Tratamento
3.
BMC Oral Health ; 19(1): 121, 2019 06 19.
Artigo em Inglês | MEDLINE | ID: mdl-31217005

RESUMO

BACKGROUND: This study aimed to assess the efficacy of a 980-nm diode and 10.6-µm CO2 laser accompanied by tricalcium phosphate-5% sodium fluoride (fTCP) and casein phosphopeptide amorphous calcium phosphate (CPP-ACP) for the remineralization of primary teeth. METHODS: In total, 117 extracted primary anterior teeth were randomly divided into eight experimental and one control group: (I) control (polished enamel), (II) fTCP varnish, (III) fTCP + diode laser, (IV) fTCP + CO2 laser, (V) CPP-ACP, (VI) CPP-ACP + diode laser, (VII) CPP-ACP + CO2 laser, (VIII) diode laser, and (IX) CO2 laser. The microhardness of 12 samples in each group and the enamel porosity of one sample in each group were assessed before and after demineralization and 28 days after remineralization. Data were analysed using two-way ANOVA. RESULTS: Significant differences existed in microhardness (P = 0.004) and percentage of remineralization (P < 0.001) after remineralization among the material groups such that the highest mean was noted in the CPP-ACP group. No significant difference was noted in microhardness (P = 0.052) or percentage of remineralization (P = 0.981) after remineralization among the laser groups. In all groups, porosities increased after demineralization and slightly decreased after remineralization; the greatest reduction in porosity of the material groups was noted in the fTCP group, and the CO2 group among the laser groups. The interaction effect of materials and lasers was not significant (P > 0.05). CONCLUSION: The highest microhardness was achieved after remineralization with CPP-ACP. The efficacy of the diode and CO2 lasers was the same. No synergistic effect was found between materials and lasers. TRIAL REGISTRATION: This is not a human subject research.


Assuntos
Cariostáticos/farmacologia , Caseínas/farmacologia , Esmalte Dentário/efeitos dos fármacos , Fluoretos/farmacologia , Lasers de Gás , Lasers Semicondutores , Remineralização Dentária/métodos , Dente Decíduo/efeitos dos fármacos , Criança , Pré-Escolar , Feminino , Humanos , Técnicas In Vitro , Masculino , Propriedades de Superfície/efeitos dos fármacos
4.
Acta Odontol Latinoam ; 32(1): 29-35, 2019 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-31206572

RESUMO

The aim of this study was to evaluate the deproteinization of primary enamel by analyzing etching pattern types, with and without the application of 5% NaOCl before acid etching with 37% H3PO4. Fifteen extracted human primary molars were randomly selected for the present in vitro study; 1mm x 1mm blocks were prepared and divided into two groups (n = 21). These groups were treated as follows: Group A- Acid Etching with 37% H3PO4 gel for 15 s; Group B- 5% NaOCl for 60 s + Acid Etching with 37% H3POfor 15 s. The specimens were prepared for scanning electron microscopy analysis. The images were evaluated for quality types I and II etching of the enamel surface using ImageJ software. Datasets were checked for normality by Kolgomorv-Smirnov test and the nonparametric unpaired Mann-Whitney test was applied. The mean surface area of type I and II etching pattern values was 1922.314 µm2for Group A and 3840.473 µm2Group B. We conclude that deproteinization with 5% NaOCl prior to acid etching can be used to increase the area of adhesion and the quality of the etching pattern.


Assuntos
Condicionamento Ácido do Dente/métodos , Colagem Dentária , Proteínas do Esmalte Dentário/efeitos dos fármacos , Esmalte Dentário/efeitos dos fármacos , Materiais Dentários/farmacologia , Ácidos Fosfóricos/farmacologia , Hipoclorito de Sódio/farmacologia , Dente Decíduo/efeitos dos fármacos , Colagem Dentária/métodos , Esmalte Dentário/ultraestrutura , Proteínas do Esmalte Dentário/ultraestrutura , Corrosão Dentária , Humanos , Microscopia Eletrônica de Varredura , Desnaturação Proteica , Cimentos de Resina , Propriedades de Superfície , Dente Decíduo/ultraestrutura
5.
Niger J Clin Pract ; 22(3): 313-319, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30837417

RESUMO

Background: Although there are several studies on permanent tooth wear caused by dental materials, studies concerning primary teeth are limited. Aim: To evaluate the wear of primary tooth enamel against different ceramic and composite resin materials. Settings and Design: In vitro study. Materials and Methods: We assessed five materials (n = 10 per group): monolithic zirconia (group Z), lithium disilicate glass ceramic (group L), resin nanoceramic (group R), nanohybrid composite resin (group C), and primary tooth enamel (group E). The mesiopalatal cusps of primary maxillary second molars were used as antagonists. Wear tests were performed in a dual-axis chewing simulator, and the volume loss in the antagonist tooth was evaluated using a laser scanner and three-dimensional profiling system. Statistical Analysis Used: Data were statistically analyzed using one-way analysis of variance with Tukey's post hoc tests (P < 0.05). Results: The maximum antagonist tooth wear was observed in group L (3.84 ± 0.7 mm3), followed by groups C (3.68 ± 0.76 mm3), R (3.48 ± 0.71 mm3), Z (2.66 ± 0.65 mm3), and E (1.66 ± 0.42 mm3). Volume loss was significantly lesser in group Z than in groups L and C (P < 0.05), whereas there were no significant differences among groups L, C, and R. Conclusion: Within the limitations of this in vitro study, our findings suggest that zirconia should be used for full coronal coverage in primary tooth restorations because it causes lesser antagonist tooth wear than does lithium disilicate, resin nanoceramic, and nanohybrid composite resin.


Assuntos
Cerâmica/farmacologia , Resinas Compostas/química , Resinas Compostas/farmacologia , Esmalte Dentário/patologia , Materiais Dentários/química , Desgaste dos Dentes/etiologia , Dente Decíduo/efeitos dos fármacos , Porcelana Dentária , Desgaste de Restauração Dentária , Análise do Estresse Dentário , Humanos , Mastigação , Teste de Materiais , Propriedades de Superfície , Desgaste dos Dentes/diagnóstico , Desgaste dos Dentes/patologia , Zircônio
6.
J Indian Soc Pedod Prev Dent ; 37(1): 60-66, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30804309

RESUMO

Background: Indirect pulp therapy (IPT) is conventional treatment of deep dentinal carious lesions without risk of pulp exposure. Development of new biomaterials with biocompatibility and seal has changed the attitudes toward IPT. Aim/Objectives: This study was conducted to evaluate and compare the clinical and radiographic success of biodentine, 2% chlorhexidine gluconate with resin-modified glass ionomer cement (RMGIC), and calcium hydroxide as indirect pulp-capping materials in primary molars over an observation period of 12 months. Materials and Methods: A total of 54 primary molars within the age group of 3-9 years, which were indicated for IPT were randomized into three groups of 18 samples each; Group I: biodentine, Group II: 2% chlorhexidine gluconate followed by RMGIC, Group III: calcium hydroxide (Dycal). These cases were followed up for evaluation clinically and radiographically at 3, 6, and 12 months. The recorded data were subjected to statistical analysis using Chi-square test. Results: After 12 months, the clinical and radiographic success of biodentine group was 100% (18/18), with 2% chlorhexidine gluconate disinfecting solution followed by RMGIC was 94.4% (17/18) and calcium hydroxide (Dycal) was 94.4% (17/18), failures included one at 3 months with RMGIC and another at 12 months with calcium hydroxide group; but there was no statistically significant difference observed between them with P = 0.361 at 3 months and P = 0.371 at 12 months interval. Conclusion: Biodentine can be effectively used as indirect pulp-capping medicament in primary teeth which has similar clinical and radiographic success as 2% chlorhexidine gluconate in conjunction with RMGIC and calcium hydroxide.


Assuntos
Compostos de Cálcio/uso terapêutico , Hidróxido de Cálcio/uso terapêutico , Clorexidina/uso terapêutico , Cimentos de Ionômeros de Vidro/uso terapêutico , Dente Molar/efeitos dos fármacos , Agentes de Capeamento da Polpa Dentária e Pulpectomia/uso terapêutico , Cárie Radicular/tratamento farmacológico , Silicatos/uso terapêutico , Dente Decíduo/efeitos dos fármacos , Criança , Pré-Escolar , Clorexidina/administração & dosagem , Humanos
7.
Lasers Med Sci ; 34(5): 881-891, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30446860

RESUMO

The aim of this in vitro study was to determine the effect of different remineralization agents and laser on caries resistance of primary enamel. In the study, 150 sound primary molars were used. The initial microhardness values were measured and the teeth were randomly assigned to ten treatment groups (n = 15): no treatment/negative control (C), NaF, APF, fluoride varnish (FV), CPP-ACP, laser (L), L + NaF, L + APF, L + FV, L + CPP-ACP. The microhardness values were measured after the treatments and the pH cycle. The obtained data were analyzed statistically. One sample from each group was examined before treatment, after treatment, and after the pH cycle with a scanning electron microscope. While microhardness values after treatment compared to baseline increased, microhardness after the pH cycle decreased compared to after treatment values in all experimental groups (p < 0.05). In regard to the difference in microhardness after the pH cycle and baseline, there were no statistically significant differences between groups C and NaF and between C and CPP-ACP (p > 0.05). There was a significant difference between groups L and L + FV (p < 0.05), while no significant difference was noted between groups L and L + NaF, L + APF, L + CPP-ACP (p > 0.05). As a conclusion, FV is more effective when used in combination with laser than laser alone. NaF, CPP-ACP, and laser may be insufficient in protecting the primary teeth against acid attacks compared to FV used with laser.


Assuntos
Caseínas/uso terapêutico , Cárie Dentária/cirurgia , Esmalte Dentário/efeitos da radiação , Fluoretos/uso terapêutico , Lasers de Estado Sólido , Dente Decíduo/efeitos dos fármacos , Dente Decíduo/efeitos da radiação , Esmalte Dentário/efeitos dos fármacos , Esmalte Dentário/ultraestrutura , Fluoretos/farmacologia , Dureza , Humanos , Concentração de Íons de Hidrogênio
8.
PLoS One ; 13(12): e0208938, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30533035

RESUMO

In nerve regeneration studies, various animal models are used to assess nerve regeneration. However, because of the difficulties in functional nerve assessment, a visceral nerve injury model is yet to be established. The superior laryngeal nerve (SLN) plays an essential role in swallowing. Although a treatment for SLN injury following trauma and surgery is desirable, no such treatment is reported in the literature. We recently reported that stem cells derived from human exfoliated deciduous teeth (SHED) have a therapeutic effect on various tissues via macrophage polarization. Here, we established a novel animal model of SLN injury. Our model was characterized as having weight loss and drinking behavior changes. In addition, the SLN lesion caused a delay in the onset of the swallowing reflex and gain of laryngeal residue in the pharynx. Systemic administration of SHED-conditioned media (SHED-CM) promoted functional recovery of the SLN and significantly promoted axonal regeneration by converting of macrophages to the anti-inflammatory M2 phenotype. In addition, SHED-CM enhanced new blood vessel formation at the injury site. Our data suggest that the administration of SHED-CM may provide therapeutic benefits for SLN injury.


Assuntos
Meios de Cultivo Condicionados/farmacologia , Transtornos de Deglutição/tratamento farmacológico , Nervos Laríngeos/crescimento & desenvolvimento , Regeneração Nervosa/efeitos dos fármacos , Animais , Polaridade Celular/efeitos dos fármacos , Polaridade Celular/genética , Criança , Deglutição/efeitos dos fármacos , Deglutição/fisiologia , Transtornos de Deglutição/fisiopatologia , Polpa Dentária/citologia , Polpa Dentária/efeitos dos fármacos , Polpa Dentária/crescimento & desenvolvimento , Modelos Animais de Doenças , Feminino , Humanos , Nervos Laríngeos/efeitos dos fármacos , Nervos Laríngeos/patologia , Macrófagos/efeitos dos fármacos , Masculino , Nervos Periféricos/efeitos dos fármacos , Nervos Periféricos/crescimento & desenvolvimento , Ratos , Regeneração , Células-Tronco/citologia , Células-Tronco/efeitos dos fármacos , Dente Decíduo/citologia , Dente Decíduo/efeitos dos fármacos
9.
Sci Rep ; 8(1): 17908, 2018 12 17.
Artigo em Inglês | MEDLINE | ID: mdl-30559343

RESUMO

Papacarie gel is an agent that eliminates the need for local anesthesia and reduces the need for using a drill. However, there is no information regarding the cost per procedure. Therefore we analyzed the cost, per procedure, of Papacarie gel compared to the traditional method (drilling), and performed a comparison between these methods of carious tissue removal. A randomized clinical trial was performed with 24 children with an average age of 5.9 years old. Of these children, 12 were boys and 12 were girls, which resulted in a total of 46 restorations. Patients were separated into: Papacarie group (caries removal with the chemical-mechanical method - Papacarie gel) and Drill group (caries removal with the traditional method - drilling). Values of the materials used in the procedures, heart rate (before, 5 minutes during, and after dental treatment), and the total consultation duration were recorded. A level of significance of 5% was adopted. Papacarie had a lower cost per procedure ($ 0.91) when compared to the traditional method ($ 1.58). Papacarie provided a cost reduction of 42% compared to the traditional method. Using local anesthesia ($ 2.17), the cost reduction increased to 58%. In the procedure using drill + Papacarie ($ 1.37), the cost reduction was 33%. Heart rate, consultation duration, and number of restorations were not statistically different. Papacarie shows an excellent cost benefit for minimally invasive removal of carious tissue and is a feasible alternative for public health care.


Assuntos
Cárie Dentária/tratamento farmacológico , Cárie Dentária/economia , Papaína/economia , Papaína/uso terapêutico , Odontopediatria/economia , Brasil , Criança , Pré-Escolar , Análise Custo-Benefício , Feminino , Humanos , Masculino , Dente Decíduo/efeitos dos fármacos
10.
Microsc Res Tech ; 81(9): 1077-1083, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30351522

RESUMO

This study evaluates, in situ, the effect of an anti-asthmatic medicine on the enamel and dentin of primary teeth. Twenty-eight specimens of enamel and dentin were prepared, selected, sterilized, and stored. Microhardness, microscopic morphology, and initial surface roughness were evaluated. Fourteen volunteers wore palatal devices containing two dentin specimens and two enamel specimens. Volunteers underwent surface treatment three times a day for 5 days, with the dripping of 5 ml of salbutamol sulfate for 1 min, only on specimens from one side of the device. At the end of the experiment the microscopic morphology, roughness, surface loss, and microhardness were ascertained. For enamel surfaces exposed to the medicine, erosion was observed in the microscopy images, along with a significant increase in roughness (p = .0325) and tissue loss (p = .03251) and a significant decrease in microhardness (p = .0325). For the dentin surfaces, erosion was observed in the microscopy images, but there was no significant increase in roughness (p = .593) or tissue loss (p = .285) nor any decrease in microhardness (p = .1088). In conclusion, the salbutamol sulfate had an in situ erosive effect on primary teeth enamel and this effect was observed morphologically on primary dentin. RESEARCH HIGHLIGHTS: Asthma is considered a global health problem, and its prevalence has increased in many countries, especially among children, as well as dental erosion. This study has clinical relevance because provides data on the erosive effect of a commonly prescribed anti asthmatic drug on deciduous teeth.


Assuntos
Antiasmáticos/efeitos adversos , Esmalte Dentário/efeitos dos fármacos , Dentina/efeitos dos fármacos , Dente Decíduo/efeitos dos fármacos , Adulto , Brasil , Feminino , Testes de Dureza , Humanos , Masculino , Microscopia , Propriedades de Superfície/efeitos dos fármacos , Adulto Jovem
11.
Int J Exp Pathol ; 99(5): 264-268, 2018 10.
Artigo em Inglês | MEDLINE | ID: mdl-30324690

RESUMO

The interradicular region of primary molars is permeated by many foramina, channels and accessories that connect the pulp cavity with the periapical tissues anatomically. Thus, pulp decomposition products or drugs used in endodontic treatment can trigger inflammatory reactions. The aim of this study was to evaluate the blood cell profile of the alveolar region after extraction of primary molars treated with CTZ paste. Forty-eight primary molars were selected with clinical and radiographic signs of extraction. The sample was divided into three groups with 16 teeth each: Group 1-healthy teeth; Group 2-untreated decayed teeth; and Group 3-teeth treated with CTZ paste. Immediately after the extraction, blood from the interface of the tooth socket was collected and smears were performed for further evaluation. The slides were stained by the Fast Panoptic® method and analysed by two previously trained examiners who counted the leucocytes in sets of 100 cells/slide, differentiating them into neutrophils, lymphocytes, monocytes, eosinophils and basophils. The data were analysed statistically by the MANOVA test. The blood samples from Group 2 differed significantly from Group 1 samples for all classes of leucocytes, except basophils, with higher average for lymphocytes (62.56), monocytes (7.81) and eosinophils (2.31). For Group 3, there was a relative difference (P < 0.05) to Group 2, of monocytes and eosinophils values. The blood cellularity interface in the tooth socket of primary teeth treated with CTZ paste is similar to those of healthy, exfoliated teeth and physiologically different from untreated decayed teeth.


Assuntos
Antibacterianos/uso terapêutico , Necrose da Polpa Dentária/tratamento farmacológico , Dente Molar/efeitos dos fármacos , Dente Decíduo/diagnóstico por imagem , Antibacterianos/administração & dosagem , Criança , Estudos Transversais , Necrose da Polpa Dentária/sangue , Humanos , Leucócitos/patologia , Dente Molar/irrigação sanguínea , Dente Molar/diagnóstico por imagem , Dente Molar/patologia , Pomadas , Alvéolo Dental/diagnóstico por imagem , Alvéolo Dental/patologia , Dente Decíduo/efeitos dos fármacos
12.
J Indian Soc Pedod Prev Dent ; 36(3): 257-261, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30246746

RESUMO

Background: International data on caries epidemiology confirm that dental caries remains a significant disease of childhood in both developing and developed countries. Based on preventive dentistry, topical fluoride may be a useful measure to arrest caries lesions. Fluoride used in various forms have been proven to be effective in dental caries prevention. Aim: This study aims to compare the effect of fluoridated varnish and silver diamine fluoride (SDF) solution on primary teeth enamel resistance to demineralization. Methods: Forty-five caries-free deciduous canine teeth extracted due to orthodontic reasons, devoid of any defects were selected. Teeth were mounted on acrylic blocks as their buccal surface was exposed and baseline surface microhardness (SMH) determination was accomplished. Enamel samples were randomly distributed into three groups with 15 specimens each. One group was used as control (distilled and deionized water) (C); in the other groups, either a fluoridated varnish (V) or an SDF solution was applied to the enamel blocks. The blocks of each group were submitted to pH-cycling solutions and treatment regimen. After pH-cycling process, SMH determination was done again for all samples. Results: According to the present findings, the percentage of decrease in SMH of control group is numerically greater than other groups and also SDF group shows the most resistance against mineral loss. However, based on one-way ANOVA test, this difference is not statistically significant (P = 0.217). Conclusion: SDF solution and fluoride varnish display similar effectiveness in preventing the demineralization of deciduous anterior teeth, and no significant difference was observed.


Assuntos
Cariostáticos/administração & dosagem , Esmalte Dentário/efeitos dos fármacos , Fluoretos Tópicos/administração & dosagem , Compostos de Amônio Quaternário/administração & dosagem , Compostos de Prata/administração & dosagem , Fluoreto de Sódio/administração & dosagem , Desmineralização do Dente/prevenção & controle , Dente Decíduo/efeitos dos fármacos , Criança , Cárie Dentária/prevenção & controle , Dureza , Humanos , Concentração de Íons de Hidrogênio , Técnicas In Vitro , Propriedades de Superfície , Dente Decíduo/fisiologia
13.
J Indian Soc Pedod Prev Dent ; 36(2): 158-166, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29970633

RESUMO

Background: Organic amine fluorides and nano-hydroxyapatite dentifrices have shown remineralization potential in various studies. However, there is a lack of direct comparison between amine fluoride and nano-hydroxyapatite with conventional inorganic fluorides as sodium monofluorophosphate. Aim: The aim of the study is to evaluate remineralizing efficacy of the three dentifrices on both primary and permanent enamel surfaces. Methods: Enamel sections were obtained from 40 sound molars - 20 primary and 20 permanent molars. Five enamel slabs were prepared from these extracted molars stored in artificial saliva, wherein one slab acted as control, second as demineralized (negative control), and other three slabs were brushed with sodium monofluorophosphate, amine fluoride, and nano-hydroxyapatite dentifrices, respectively, twice daily for 21 days. Scanning electron microscopy- Energy dispersive spectroscopy (SEM-EDS) analysis for surface morphology and calcium and phosphorus content and Vickers surface microhardness (SMH) values were evaluated at baseline, after demineralization, and postapplication of the experimental agents. Results: Highly significant changes in calcium phosphorus ratio and SMH values were seen in enamel slabs treated with nano-hydroxyapatite and amine fluoride dentifrice as compared to sodium monofluorophosphate in both primary and permanent teeth. Surface morphology of enamel slabs treated with amine fluoride most closely resembled natural enamel morphology, while sodium monofluorophosphate-treated surfaces showed globular pattern of remineralization. Deposition of a separate highly mineralized layer over existing surface was seen in nano-hydroxyapatite-treated surfaces. Conclusions: Nano-hydroxyapatite exhibited highest remineralization potential in terms of mineral gain followed by amine fluoride and sodium monofluorophosphate dentifrice.


Assuntos
Esmalte Dentário/efeitos dos fármacos , Dentifrícios/farmacologia , Durapatita/farmacologia , Fluoretos/farmacologia , Fosfatos/farmacologia , Remineralização Dentária , Aminas , Esmalte Dentário/ultraestrutura , Dureza , Humanos , Concentração de Íons de Hidrogênio , Estudos Prospectivos , Dente/efeitos dos fármacos , Dente/ultraestrutura , Dente Decíduo/efeitos dos fármacos , Dente Decíduo/ultraestrutura
14.
Photochem Photobiol ; 94(6): 1240-1248, 2018 11.
Artigo em Inglês | MEDLINE | ID: mdl-29923618

RESUMO

This study aimed to evaluate the use of antimicrobial photodynamic therapy (aPDT) as an adjunct for minimally invasive treatment (partial removal of carious tissue-PRCT) of deciduous carious tissue evaluating its efficacy in reducing microorganisms. For that, a clinical study was design including children with deciduous molars with active deep caries lesions (DCL). PRCT was performed and remaining dentin was treated with 100 µg mL-1 methylene blue solution (5 min) and than irradiated with a low power laser emitting red light (InGaAIP-indium gallium aluminum phosphide; λ = 660 nm; 100 mW; 300 J cm-2 ; 90 s; 9 J). The colony forming units (CFU) count after PRCT and after PRCT + aPDT/mg of dentin were compared for total microorganisms, including Candida spp., the mutans streptococci group, Streptococcus spp. and Lactobacillus spp. The dentin was classified (color, consistency and humidity). The microbial reduction varied from 69.88% to 86.29% and was significantly observed for total microorganisms, mutans streptococci, Streptococcus spp. and Lactobacillus spp (P < 0.001). The dentin type did not influence reduction of microorganisms (P > 0.05). The aPDT presents a promising future for clinical use as an adjunct for the reduction of microorganisms in PRCT of DCL in all kinds of dentin.


Assuntos
Cárie Dentária/tratamento farmacológico , Azul de Metileno/administração & dosagem , Dente Molar/efeitos dos fármacos , Fotoquimioterapia/métodos , Fármacos Fotossensibilizantes/administração & dosagem , Dente Decíduo/efeitos dos fármacos , Candida/efeitos dos fármacos , Candida/crescimento & desenvolvimento , Criança , Pré-Escolar , Cárie Dentária/microbiologia , Cárie Dentária/cirurgia , Esmalte Dentário/efeitos dos fármacos , Esmalte Dentário/microbiologia , Esmalte Dentário/cirurgia , Dentina/efeitos dos fármacos , Dentina/microbiologia , Dentina/cirurgia , Feminino , Humanos , Lactobacillus/efeitos dos fármacos , Lactobacillus/crescimento & desenvolvimento , Lasers , Luz , Masculino , Azul de Metileno/metabolismo , Procedimentos Cirúrgicos Minimamente Invasivos/métodos , Dente Molar/microbiologia , Dente Molar/cirurgia , Fármacos Fotossensibilizantes/metabolismo , Streptococcus/efeitos dos fármacos , Streptococcus/crescimento & desenvolvimento , Dente Decíduo/microbiologia , Dente Decíduo/cirurgia , Resultado do Tratamento
15.
J Contemp Dent Pract ; 19(4): 404-408, 2018 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-29728544

RESUMO

Introduction: Modifying the durability of resin bonding systems resulted in introducing materials with cross-link property, such as chlorhexidine (CHX) that can promote the strength of resin-dentin bonding. The aim of this study was evaluation of the effect of CHX on shear bond strength (SBS) of self-etch (SE) and etch-and-rinse (E&R) bonding systems to the primary teeth dentin. Materials and methods: In this in vitro study, 40 freshly extracted primary molars collected and were cut mesiodistally, and then the buccal and lingual surface enamel was grounded. The specimens were randomly divided into two groups: I: Single Bond adhesive and II: Clearfil SE Bond adhesive. Then, each group was divided into two subgroups: On the buccal surface of each sample CHX was applied for 30 seconds and on the lingual surface of (I2, II2 groups), CHX was not applied. The SBS (MPa) of samples was assessed using the Zwick universal testing machine. Data were analyzed by paired t-test and independent t-test using Statistical Package for the Social Sciences (SPSS), version 16. Results: The mean SBS was significantly higher in the Single Bond group with CHX (66.45 ± 8.3) than without CHX (25.43 ± 12.94) in the nonaging group (p = 0.002). There was no significant difference in the mean SBS in the Clearfil SE Bond group with CHX and without CHX in the aging and nonaging groups. Conclusion: CHX had positive influence on immediate SBS of Single Bond adhesive to primary teeth dentin. In addition, no positive effect has been seen in the Clearfil SE Bond system. Clinical significance: Now more researches are needed about the recommendation of CHX in resin restorations of primary teeth. Keywords: Chlorhexidine, Etch-and-rinse adhesive, Failure pattern, Self-etch adhesive, Shear bond strength.


Assuntos
Clorexidina/uso terapêutico , Colagem Dentária/métodos , Corrosão Dentária/métodos , Dentina/efeitos dos fármacos , Desinfetantes/uso terapêutico , Dente Decíduo/efeitos dos fármacos , Bis-Fenol A-Glicidil Metacrilato/uso terapêutico , Restauração Dentária Permanente/métodos , Análise do Estresse Dentário , Humanos , Técnicas In Vitro , Cimentos de Resina/uso terapêutico
16.
J Appl Oral Sci ; 26: e20160629, 2018 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-29412365

RESUMO

The aim of the study was to evaluate the effects of the capping materials mineral trioxide aggregate (MTA), calcium hydroxide (CH) and BiodentineTM (BD) on stem cells from human exfoliated deciduous teeth (SHED) in vitro. SHED were cultured for 1 - 7 days in medium conditioned by incubation with MTA, BD or CH (1 mg/mL), and tested for viability (MTT assay) and proliferation (SRB assay). Also, the migration of serum-starved SHED towards conditioned media was assayed in companion plates, with 8 µm-pore-sized membranes, for 24 h. Gene expression of dentin matrix protein-1 (DMP-1) was evaluated by reverse-transcription polymerase chain reaction. Regular culture medium with 10% FBS (without conditioning) and culture medium supplemented with 20% FBS were used as controls. MTA, CH and BD conditioned media maintained cell viability and allowed continuous SHED proliferation, with CH conditioned medium causing the highest positive effect on proliferation at the end of the treatment period (compared with BD and MTA) (p<0.05). In contrast, we observed increased SHED migration towards BD and MTA conditioned media (compared with CH) (p<0.05). A greater amount of DMP-1 gene was expressed in MTA group compared with the other groups from day 7 up to day 21. Our results show that the three capping materials are biocompatible, maintain viability and stimulate proliferation, migration and differentiation in a key dental stem cell population.


Assuntos
Compostos de Alumínio/farmacologia , Compostos de Cálcio/farmacologia , Hidróxido de Cálcio/farmacologia , Óxidos/farmacologia , Agentes de Capeamento da Polpa Dentária e Pulpectomia/farmacologia , Silicatos/farmacologia , Células-Tronco/efeitos dos fármacos , Dente Decíduo/citologia , Análise de Variância , Diferenciação Celular/efeitos dos fármacos , Movimento Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Células Cultivadas , Capeamento da Polpa Dentária/métodos , Combinação de Medicamentos , Proteínas da Matriz Extracelular/análise , Gliceraldeído-3-Fosfato Desidrogenases/efeitos dos fármacos , Humanos , Teste de Materiais , Fosfoproteínas/análise , Reprodutibilidade dos Testes , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Células-Tronco/fisiologia , Fatores de Tempo , Dente Decíduo/efeitos dos fármacos
17.
Photomed Laser Surg ; 36(4): 179-184, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29480759

RESUMO

BACKGROUND: A successful primary root canal treatment depends on effective shaping and cleaning the root canal system and finally filling it with a hermetic sealer. Clinically, roots of primary teeth are difficult to shape and the irrigation/disinfection protocol has great importance on prognosis. OBJECTIVE: The present study evaluated the antibacterial efficiency of Endosafe (Orangedental GmbH & Co. KG), photo-activated disinfection (PAD; Orangedental GmbH & Co. KG), diode laser (Epic 10; Biolase, Inc.), ozone (O3, Ozonytron; Biozonix, München, Germany), and sodium hypochloride applications in primary root canals that were infected with Enterococcus faecalis after standard mechanical instrumentation. METHODS: The study was conducted on roots of 100 human primary molar teeth, which were extracted due to excessive caries. The roots were divided in 5 groups with 15 roots in each root disinfection protocol. In addition, 15 samples and 10 samples served as positive and negative controls, respectively. The Shapiro-Wilk test, the Kruskal-Wallis test and then by post hoc group comparisons with the Bonferroni-adjusted Mann-Whitney U test (unpaired observations) was used. RESULTS: Sodium hypochlorite (NaOCl) exhibited the highest antibacterial effect (0 colony-forming units per mL). Diode laser irradiation was statistically more effective than the ozone, PAD, and Endosafe groups (p < 0.001). Endosafe, PAD, and ozone groups showed similar antibacterial effect (p > 0.05). Although not statistically significant, the Endosafe was more effective in reducing the bacterial count when compared with ozone and PAD. CONCLUSIONS: The five tested irrigation systems were shown to be effective in disinfection of the E. faecalis-contaminated primary root canals and best results were obtained with 2.5% NaOCl and diode laser.


Assuntos
Desinfecção , Lasers Semicondutores/uso terapêutico , Terapia com Luz de Baixa Intensidade , Preparo de Canal Radicular/métodos , Dente Decíduo/efeitos dos fármacos , Dente Decíduo/efeitos da radiação , Desinfetantes/uso terapêutico , Enterococcus faecalis , Humanos , Oxidantes Fotoquímicos/uso terapêutico , Ozônio/uso terapêutico , Irrigantes do Canal Radicular , Hipoclorito de Sódio/uso terapêutico , Técnicas de Cultura de Tecidos , Dente Decíduo/microbiologia
18.
J Dent ; 70: 74-79, 2018 03.
Artigo em Inglês | MEDLINE | ID: mdl-29289726

RESUMO

OBJECTIVE: To compare the effectiveness of three applications of silver diammine fluoride (SDF) solution at yearly interval and three applications of SDF solution or sodium fluoride (NaF) varnish at weekly interval at baseline in arresting active caries in the primary teeth of preschool children. METHODS: Children aged 3-4 years (n = 371) who had at least one active caries lesion (ICDAS codes 3-6) in their primary teeth were randomly allocated into three groups: Group 1 - annual application of 30% SDF solution; Group 2 - three applications of 30% SDF at weekly intervals; and Group 3 - three applications of 5% NaF varnish at weekly intervals. Follow-up examinations were performed every 6 mo nths by the same masked examiner. RESULTS: After 30 months, 309 (83%) children with 1877 caries lesions remained in the study. For cavitated lesions (ICDAS code 5 or 6), the caries arrest rate of Group 1 (48%) was significantly higher than those of Group 2 (33%) and Group 3 (34%), (p < 0.001). Results of multi-level survival analysis showed that the arrest times of cavitated lesions in both SDF groups (Groups 1 and 2) were significantly shorter than that of the NaF varnish group. For moderate caries lesions without visible dentine (ICDAS code 3 or 4), the caries arrest rates were 45%, 44% and 51% in Groups 1, 2 and 3, respectively (p > 0.05). Presence of plaque on caries lesion, tooth type and tooth surface type had an influence on caries arrest. CONCLUSION: Over a 30-month period, annual applications of SDF solution is more effective than three weekly applications of NaF varnish or SDF solution at baseline in arresting active cavitated dentine caries lesions in primary teeth. CLINICAL SIGNIFICANCE: As annual application of SDF solution was found to be more effective than 3 weekly applications of NaF varnish or SDF solution at baseline in arresting active cavitated dentine caries lesions, the former application protocol is preferred for young children who are available for regular caries arrest treatment.


Assuntos
Cárie Dentária/tratamento farmacológico , Fluoretos Tópicos/administração & dosagem , Fluoretos Tópicos/uso terapêutico , Cariostáticos/uso terapêutico , Pré-Escolar , Cárie Dentária/patologia , Dentina/efeitos dos fármacos , Dentina/patologia , Feminino , Hong Kong , Humanos , Modelos Logísticos , Masculino , Compostos de Amônio Quaternário/uso terapêutico , Análise de Regressão , Compostos de Prata/uso terapêutico , Fluoreto de Sódio/uso terapêutico , Análise de Sobrevida , Dente Decíduo/efeitos dos fármacos
19.
J Appl Oral Sci ; 26: e20170053, 2018 Jan 18.
Artigo em Inglês | MEDLINE | ID: mdl-29364339

RESUMO

To evaluate the effect of erosive challenges on the tooth- restoration interface of deciduous teeth treated with different adhesive protocols. Deciduous molars were cut mesiodistally, then embedded, abraded and polished (n=80). Samples were randomly divided according to the adhesive system used into: G1 (Adper Single Bond2®, etch-and-rinse), G2 (Universal Single Bond®, self-etching), G3 (OptibondFL®, etch-and-rinse with Fluoride) and G4 (BondForce®, self-etching with Fluoride). After standardized cavity preparation (2 mm diameter x 2 mm depth), adhesive systems were applied and samples were restored (composite resin Z350®). Half of the samples were exposed to erosive/abrasive cycles (n = 10, each adhesive group), and the other half (control group; n = 10) remained immersed in artificial saliva. For microleakage analysis, samples were submersed in methylene blue and analyzed at 40x magnifications. Cross-sectional microhardness (CSMH) was carried out (50 g/5 s) at 25 µm, 50 µm, and 100 µm from the eroded surface and at 25 µm, 75 µm, and 125 µm from the enamel bond interface. Regarding microleakage, 7.5% of the samples showed no dye infiltration, 30% showed dye infiltration only at the enamel interface, and 62.5% showed dye infiltration through the dentin-enamel junction, with no difference between groups (p≥0.05). No significant difference was observed in CSMH at different depths (two-way ANOVA, p≥0.05). We did not observe significant changes in microleakage or CSMH after erosive/abrasive challenges in deciduous teeth treated with different adhesive protocols (etch-and-rinse and self-etching adhesives, with and without fluoride).


Assuntos
Bis-Fenol A-Glicidil Metacrilato/química , Resinas Compostas/química , Cimentos Dentários/química , Cimentos de Resina/química , Erosão Dentária/etiologia , Dente Decíduo/química , Análise de Variância , Esmalte Dentário/química , Esmalte Dentário/efeitos dos fármacos , Infiltração Dentária/etiologia , Dentina/química , Dentina/efeitos dos fármacos , Fluoretos/química , Testes de Dureza , Humanos , Teste de Materiais , Distribuição Aleatória , Valores de Referência , Reprodutibilidade dos Testes , Saliva Artificial/química , Estatísticas não Paramétricas , Propriedades de Superfície , Dente Decíduo/efeitos dos fármacos
20.
J Clin Pediatr Dent ; 42(2): 146-149, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29087792

RESUMO

OBJECTIVES: The aim of the present study was to assess the effect of calcium hydroxide as a root canal dressing material on dentin microtensile fracture strength in human primary teeth in vitro. STUDY DESIGN: Thirty primary anterior teeth with root canals packed with calcium hydroxide were divided into groups of ten and immersed in saline at room temperature for 7, 30 and 90 days. Ten teeth with root canals filled with sterile saline were the control group. Microtensile fracture strength was measured in Mechanical tester Lloyd testing machine. RESULTS: There was a significant difference (P < 0.05) between the fracture strength of the calcium hydroxide-filled teeth after 90 days (19.1 MPa) compared with the control (35.8 MPa). Dentin microtensile fracture strength of the calcium hydroxide-filled teeth decreased at an average of 0.142 MPa per day. CONCLUSION: Calcium hydroxide placed in root canals for an extended time had a significantly negative effect on root strength. Long-term success of root canal treatment in primary anterior teeth is estimated as 65% with most of the failures result from trauma recurrence. CLINICAL RELEVANCE: Our results stress the need to evaluate the pros and cons of root canal treatment compared to extractions of non-vital primary incisors.


Assuntos
Hidróxido de Cálcio/farmacologia , Cavidade Pulpar/efeitos dos fármacos , Dentina/efeitos dos fármacos , Dente Decíduo/efeitos dos fármacos , Bandagens , Humanos , Técnicas In Vitro , Resistência à Tração/efeitos dos fármacos , Fraturas dos Dentes
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