Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 1.733
Filtrar
1.
J Dent ; 122: 104145, 2022 07.
Artigo em Inglês | MEDLINE | ID: mdl-35523378

RESUMO

OBJECTIVES: The aim of this study was to evaluate the use of digital bitewing radiographs using photostimulable phosphor (PSP) plates in the estimation of the true extension of proximal carious lesions. METHODS: The sample size was calculated to be 70 teeth. Adult patients with a minimum of one posterior tooth with primary proximal caries lesions were included. A preoperative bitewing radiograph was taken and the extension of the carious lesion was measured from the most prominent point on the adjacent sound tooth to the deepest axial point of the lesion. An experienced general dentist provided the restorative treatment and made a polyvinyl siloxane impression of the cavity preparation. Clinical caries extension was measured from the most prominent point of the adjacent tooth until the deepest boundary of the axial wall of the preparation on the impression. Postoperative radiographs were taken at the completion of restorative care. The bitewing radiographs were done using PSP plates and caries extension measurements were performed using measuring tools of the digital radiography software. Pairwise comparisons were made between preoperative and postoperative radiographs, and the true clinical depth measurements using paired t-tests. RESULTS: In total, 73 teeth from 44 subjects were included. A significant difference of 0.82 mm was found between preoperative radiographs and clinical measurements (p<0.001). Similarly, comparisons between preoperative and postoperative radiographic measurements showed significant differences of 0.99 mm (p<0.001). CONCLUSIONS: The study found that the true clinical extension of proximal caries after non-selective caries removal was significantly deeper than the preoperative radiographic extension assessment. This was not influenced by the tooth type, the location of the tooth, or the affected tooth surfaces. CLINICAL SIGNIFICANCE: When a non-selective caries removal strategy is adopted, clinicians should be aware that PSP bitewing radiographs underestimate the true clinical extension of proximal caries lesions. This will have an impact on clinicians' practice and restorative treatment decisions.


Assuntos
Cárie Dentária , Dente , Adulto , Cárie Dentária/diagnóstico por imagem , Cárie Dentária/patologia , Cárie Dentária/terapia , Preparo da Cavidade Dentária , Humanos , Radiografia Interproximal , Radiografia Dentária Digital , Dente/patologia
2.
Int J Paleopathol ; 37: 53-59, 2022 06.
Artigo em Inglês | MEDLINE | ID: mdl-35489279

RESUMO

OBJECTIVE: This paper describes the dental elements (i.e., teeth and incomplete tooth-like structures) formed by mature cystic teratomas and provide some diagnostic criteria to aid in their paleopathological identification and analysis. MATERIALS: Hard tissue structures from 13 clinically-derived mature cystic teratomas excised between 2003 and 2007. All are from female patients (N = 13) between the ages of 17 and 56. METHODS: A dental and osteological inventory identified number of teeth and tooth types present in each specimen. RESULTS: Of the 13 specimens, nine contained dental elements. The number per teratoma ranged from one to seven. A wide variety of tooth types were present in the teratomas with some teeth independent of, and others embedded within, bony matrices. CONCLUSIONS: While some teratoma teeth exhibit distinguishable types and laterality, the majority will exhibit some degree of abnormality in size, form, developmental completion, or association with other irregular bony elements. SIGNIFICANCE: Give the modern clinical prevalence and yet relatively few paleopathological cases reported, it is likely that mature teratomas are under-identified in the archeological record. The diagnostic criteria presented in this paper can aid paleopathologists in their identification and analysis of these neoplasms. LIMITATIONS: Precise paleopathological identification of teratoma elements can be difficult; particularly when they are no longer constrained or contextualized by unmineralized neoplastic borders. As such, there is a risk that such remnants may be mistaken for fetal parts or disassociated elements. SUGGESTIONS FOR FURTHER RESEARCH: Application of these criteria, paired with careful excavation techniques, is advised.


Assuntos
Neoplasias Ovarianas , Teratoma , Dente , Adolescente , Adulto , Feminino , Cabeça/patologia , Humanos , Pessoa de Meia-Idade , Neoplasias Ovarianas/patologia , Teratoma/patologia , Dente/patologia , Adulto Jovem
3.
Sci Rep ; 12(1): 6139, 2022 04 12.
Artigo em Inglês | MEDLINE | ID: mdl-35414687

RESUMO

This study evaluated dental caries detection ability between the Qraycam and Qraypen on the same dental caries lesions. A total of 178teeth from 61patients were imaged using Qraypen C®(QC) and Qraycam Pro®(QP) devices and evaluated using analysis software (QA2). Occlusal, secondary, and proximal dental caries were evaluated and scored according to International Caries Detection and Assessment System(ICDAS II) and X-ray criteria. Bland-Altman plots were used to compare quantitative light-induced fluorescence(QLF) parameters obtained from the different QLF devices. Sensitivity, specificity, and area under the receiver operating characteristic curve(AUROC) were calculated. The ΔFaver. of the QLF-parameters showed that the mean difference between the two different QLF devices was close to zero and that the ± 5 error value was included in the mean ± 1.96SD range for the detection of dental caries. The accuracies for diagnosing occlusal dental caries were 0.83-0.96 and 0.81-0.82 and the accuracies for diagnosing proximal dental caries were 0.52-0.62 and 0.52-0.71 for the QC and QP devices, respectively. In conclusion, the ΔFaver. obtained from the QP showed diagnostic value mainly for screening of demineralized teeth. For teeth selected through screening, the depth of the lesion must be precisely evaluated using additional QP and radiographic imaging.


Assuntos
Cárie Dentária , Fluorescência Quantitativa Induzida por Luz , Dente , Cárie Dentária/diagnóstico por imagem , Cárie Dentária/patologia , Fluorescência , Humanos , Programas de Rastreamento , Curva ROC , Sensibilidade e Especificidade , Dente/patologia
4.
Equine Vet J ; 54(3): 541-548, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-34060137

RESUMO

BACKGROUND: Sagittal fractures of equine cheek teeth are commonly observed during oral examination. There are few reports on the apical and endodontic pathology associated with such fractures seen during computed tomographic (CT) examination. OBJECTIVES: This study aimed to document the prevalence of CT changes indicative of apical disease in equine cheek teeth, which have suffered a sagittal fracture involving the clinical ± reserve crown. STUDY DESIGN: This study is a retrospective case series. METHODS: CT examinations of equine heads with sagittal fractures of cheek teeth present were reviewed: 81 teeth from 49 horses were identified to have a sagittal cheek tooth fracture. The images were evaluated for apical pathology including gas (in the endodontic system and periapically), widened periodontal space, periapical sclerosis, apical clubbing, cementoma/hypercementosis, lamina dura loss, associated sinusitis and sinus mucosal swelling. An apical infection grading system was created to give each tooth a score. Hounsfield units were used to measure the density of the endodontic, apical and periapical regions. The fracture length ratio was recorded. Statistical analysis was performed using a generalised estimating equation to evaluate predictors of apical infection and associations between clinical signs and CT abnormalities. RESULTS: Eighty-seven sagittal fractures (56 buccal, 17 palatal/lingual and 14 midline) from 81 teeth were recorded (74 maxillary and 7 mandibular). Apical infection was diagnosed in 73% (37/51, P = .05) of buccal, 55% (6/11, P = .07) of palatal/lingual, 100% (13/13) of midline, 100% (6/6) of multiple fractures and 96% (23/24, P = .008) of fractures involving infundibula. There was no significant relationship between apical infection and the presence of clinical signs associated with dental pathology (P = .4). There was no significant association between fracture length ratio and apical infection (P = 1.0). Midline sagittal fractures were significantly associated with sinusitis when compared with all other maxillary fractures (odds ratio [OR] 5.92, 95% confidence interval [CI] 1.67-20.83, P = .006). Loss of the lamina dura was not significantly associated with apical infection (P = .5). MAIN LIMITATIONS: There is a maxillary cheek tooth bias in the data set and the subjective grading system. CONCLUSIONS: A large proportion of fractured cheek teeth have evidence of apical infection on CT examination and therefore warrant treatment.


Assuntos
Fraturas Ósseas , Doenças dos Cavalos , Dente , Animais , Bochecha/patologia , Fraturas Ósseas/patologia , Fraturas Ósseas/veterinária , Doenças dos Cavalos/patologia , Cavalos , Estudos Retrospectivos , Tomografia Computadorizada por Raios X/veterinária , Dente/patologia
5.
Int J Mol Sci ; 22(23)2021 Nov 24.
Artigo em Inglês | MEDLINE | ID: mdl-34884488

RESUMO

In dental pulp, diverse types of cells mediate the dental pulp immunity in a highly complex and dynamic manner. Yet, 3D spatiotemporal changes of various pulpal immune cells dynamically reacting against foreign pathogens during immune response have not been well characterized. It is partly due to the technical difficulty in detailed 3D comprehensive cellular-level observation of dental pulp in whole intact tooth beyond the conventional histological analysis using thin tooth slices. In this work, we validated the optical clearing technique based on modified Murray's clear as a valuable tool for a comprehensive cellular-level analysis of dental pulp. Utilizing the optical clearing, we successfully achieved a 3D visualization of CD11c+ dendritic cells in the dentin-pulp complex of a whole intact murine tooth. Notably, a small population of unique CD11c+ dendritic cells extending long cytoplasmic processes into the dentinal tubule while located at the dentin-pulp interface like odontoblasts were clearly visualized. 3D visualization of whole murine tooth enabled a reliable observation of these rarely existing cells with a total number less than a couple of tens in one tooth. These CD11c+ dendritic cells with processes in the dentinal tubule were significantly increased in the dental pulpitis model induced by mechanical and chemical irritation. Additionally, the 3D visualization revealed a distinct spatial 3D arrangement of pulpal CD11c+ cells in the pulp into a front-line barrier-like formation in the pulp within 12 h after the irritation. Collectively, these observations demonstrated the unique capability of optical clearing-based comprehensive 3D cellular-level visualization of the whole tooth as an efficient method to analyze 3D spatiotemporal changes of various pulpal cells in normal and pathological conditions.


Assuntos
Antígeno CD11c/metabolismo , Células Dendríticas/imunologia , Polpa Dentária/imunologia , Imageamento Tridimensional/métodos , Pulpite/imunologia , Dente/imunologia , Animais , Células Dendríticas/metabolismo , Células Dendríticas/patologia , Polpa Dentária/metabolismo , Polpa Dentária/patologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Pulpite/metabolismo , Pulpite/patologia , Dente/metabolismo , Dente/patologia
6.
Nutrients ; 13(11)2021 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-34836422

RESUMO

The aim of the study was to investigate the relationship between specific known dietary patterns and the prevalence of periodontal disease in a northern population-based cohort study. We evaluated data from 6209 participants of the Hamburg City Health Study (HCHS). The HCHS is a prospective cohort study and is registered at ClinicalTrial.gov (NCT03934957). Dietary intake was assessed with the food frequency questionnaire (FFQ2). Periodontal examination included probing depth, gingival recession, plaque index, and bleeding on probing. Descriptive analyses were stratified by periodontitis severity. Ordinal logistic regression models were used to determine the association. Ordinal regression analyses revealed a significant association between higher adherence to the DASH diet/Mediterranean diet and lower odds to be affected by periodontal diseases in an unadjusted model (OR: 0.92; 95% CI: 0.87, 0.97; p < 0.001/OR: 0.93; 95% CI: 0.91, 0.96; p < 0.001) and an adjusted model (age, sex, diabetes) (OR: 0.94; 95% CI: 0.89, 1.00; p < 0.0365/OR: 0.97; 95% CI: 0.94, 1.00; p < 0.0359). The current cross-sectional study identified a significant association between higher adherence to the DASH and Mediterranean diets and lower odds to be affected by periodontal diseases (irrespective of disease severity). Future randomized controlled trials are needed to evaluate to which extent macro- and micronutrition can affect periodontitis initiation/progression.


Assuntos
Comportamento Alimentar , Periodontite/epidemiologia , Idoso , Estudos de Coortes , Estudos Transversais , Inquéritos sobre Dietas , Dieta Mediterrânea , Abordagens Dietéticas para Conter a Hipertensão/métodos , Feminino , Humanos , Modelos Logísticos , Masculino , Pessoa de Meia-Idade , Saúde Bucal , Doenças Periodontais/epidemiologia , Prevalência , Estudos Prospectivos , Índice de Gravidade de Doença , Dente/patologia
8.
BMC Vet Res ; 17(1): 343, 2021 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-34717609

RESUMO

BACKGROUND: Prevotella histicola is a facultative oral pathogen that under certain conditions causes pathologies such as caries and periodontitis in humans. Prevotella spp. also colonize the oral cavity of horses and can cause disease, but P. histicola has not yet been identified. CASE PRESENTATION: A 12-year-old Tinker mare was referred to the clinic for persistent, malodorous purulent nasal discharge and quidding. Conservative antibiotic (penicillin), antiphlogistic (meloxicam), and mucolytic (dembrexine-hydrochloride) treatment prior to referral was unsuccessful and symptoms worsened. Oral examination, radiography, sino-/ rhinoscopy, and standing computed tomography revealed severe apical/ periapical infection of the upper cheek tooth 209 with accompanying unilateral sinonasal inflammation and conchal necrosis. The tooth exhibited extensive subocclusal mesial infundibular cemental hypoplasia and caries, and an occlusal fissure fracture. After mechanical debridement and thermoplastic resin filling of the spacious subocclusal carious infundibular lesion, the tooth was extracted intraorally. The sinusitis and conchal necrosis were treated transendoscopically. Selective bacteriological swab cultures of affected tooth roots and subsequent matrix-assisted laser desorption ionization-time of flight mass spectrometry showed an infection with the obligate anaerobic, Gram-negative bacterium P. histicola. Surgical intervention and adapted antibiotic therapy led to normal healing without complications. CONCLUSIONS: This study provides the first documented case of dental infection in a horse caused by P. histicola at once indicating necessity of more sufficient microbiological diagnostics and targeted antibiotic treatment in equine dental practice. This finding is also conducive to understand species-specific Prevotella diversity and cross-species distribution.


Assuntos
Infecções por Bacteroidaceae/diagnóstico por imagem , Infecções por Bacteroidaceae/veterinária , Doenças dos Cavalos/diagnóstico por imagem , Prevotella/isolamento & purificação , Animais , Infecções por Bacteroidaceae/patologia , Infecções por Bacteroidaceae/cirurgia , Bochecha/diagnóstico por imagem , Bochecha/microbiologia , Bochecha/patologia , Feminino , Doenças dos Cavalos/patologia , Doenças dos Cavalos/cirurgia , Cavalos , Humanos , Tomografia Computadorizada por Raios X/veterinária , Dente/diagnóstico por imagem , Dente/microbiologia , Dente/patologia , Extração Dentária/veterinária , Resultado do Tratamento
9.
Sci Rep ; 11(1): 20958, 2021 10 25.
Artigo em Inglês | MEDLINE | ID: mdl-34697324

RESUMO

Vitamin D deficiency has hugely impacted the health of past societies. Its identification in skeletal remains provides insights into the daily activities, cultural habits, and the disease load of past populations. However, up till now, this approach remained impossible in cremated bones, because temperatures reached during cremations destroyed all macroscopic evidence of vitamin D deficiency. This precluded the analyses of a large fraction of the archaeological record, as cremation was an important burial ritual from the Late Neolithic until the Early Medieval period in Europe. Here, the identification of interglobular dentine (IGD), a dental mineralisation defect attributed to vitamin D deficiency, in experimentally burnt teeth, demonstrates this deficiency to be observable in human teeth burned to temperatures as high as 900 °C. In most cases, it becomes even possible to assess the ages-of-occurrence as well as the severity of the IGD and possibly vitamin D deficiency intensity. This study represents a major step forward in the fields of biological anthropology, archaeology, and palaeopathology by opening up a variety of new possibilities for the study of health and activities related to sunlight exposure of numerous past populations that practiced cremation as their funerary ritual.


Assuntos
Dentina/patologia , Dente/química , Deficiência de Vitamina D/diagnóstico , Arqueologia , Restos Mortais/química , Cremação , Europa (Continente) , Humanos , Paleopatologia , Dente/patologia , Deficiência de Vitamina D/patologia
10.
Biomed Res Int ; 2021: 3766641, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34471637

RESUMO

Tooth bleaching is becoming increasingly popular among patients with tooth staining, but the safety of bleaching agents on tooth structure has been questioned. Primarily thriving on the biofilm formation on enamel surface, Streptococcus mutans has been recognized as a major cariogenic bacterial species. The present study is aimed at investigating how cold-light bleaching would change enamel roughness and adhesion of Streptococcus mutans. Human premolars were divided into 72 enamel slices and allocated into 3 groups: (1) control, (2) cold-light bleaching with 35% hydrogen peroxide (Beyond™), and (3) 35% hydrogen peroxide (Beyond™) alone. Biofilms of Streptococcus mutans were cultivated on enamel slices in 5% CO2 (v/v) at 37°C for 1 day or 3 days. Enamel surfaces and biofilms were observed using scanning electron microscope (SEM). Atomic force microscopy (AFM) was applied to quantify the roughness of enamel surface, and the amounts of biofilms were measured by optical density of scattered biofilm and confocal laser scanning microscopy (CLSM). Cold-light bleaching significantly increased (p < 0.05) surface roughness of enamel compared to controls, but significantly inhibited (p < 0.05) adhesion of Streptococcus mutans on enamel in the bacterial cultures of both 1 day and 3 days. In conclusion, cold-light bleaching could roughen enamel surface but inhibit Streptococcus mutans adhesion at the preliminary stage after the bleaching treatment.


Assuntos
Esmalte Dentário/efeitos dos fármacos , Peróxido de Hidrogênio/farmacologia , Streptococcus mutans/efeitos dos fármacos , Streptococcus mutans/fisiologia , Clareamento Dental/métodos , Dente/efeitos dos fármacos , Dente/microbiologia , Aderência Bacteriana , Biofilmes/efeitos dos fármacos , Biofilmes/crescimento & desenvolvimento , Esmalte Dentário/microbiologia , Esmalte Dentário/patologia , Humanos , Luz , Microscopia de Força Atômica/métodos , Medicamentos Compostos contra Resfriado, Influenza e Alergia , Propriedades de Superfície , Dente/patologia
11.
Carbohydr Polym ; 269: 118276, 2021 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-34294308

RESUMO

The guided tissue regeneration technique is an effective approach to repair periodontal defect. However, collagen barrier membranes used clinically lose stability easily, leading to soft tissue invasion, surgical site infection, and failure of osteogenesis. An ideal barrier membrane should possess proper antibacterial, osteoconductive activities, and favorable biodegradation. In this study, zinc oxide nanoparticles were homogeneously incorporated into the chitin hydrogel (ChT-1%ZnO) through one-step dissolution and regeneration method from alkaline/urea solution the first time. The remaining weights of ChT-1%ZnO in 150 µg/mL lysozyme solution was 52% after 5 weeks soaking. ChT-1%ZnO showed statistical antibacterial activities for P. gingivalis and S. aureus at 6 h, 12 h, and 24 h. Moreover, ChT-1%ZnO exhibits osteogenesis promotion in vitro, and it was further evaluated with rat periodontal defect model in vivo. The cemento-enamel junction value in ChT-1%ZnO group is 1.608 mm, presenting a statistical difference compared with no-membrane (1.825 mm) and ChT group (1.685 mm) after 8 weeks postoperatively.


Assuntos
Antibacterianos/uso terapêutico , Quitina/uso terapêutico , Hidrogéis/uso terapêutico , Membranas Artificiais , Osteogênese/efeitos dos fármacos , Doenças Periodontais/tratamento farmacológico , Animais , Antibacterianos/química , Antibacterianos/toxicidade , Proliferação de Células/efeitos dos fármacos , Quitina/química , Quitina/toxicidade , Feminino , Regeneração Tecidual Guiada/métodos , Hidrogéis/química , Hidrogéis/toxicidade , Testes de Sensibilidade Microbiana , Doenças Periodontais/patologia , Porphyromonas gingivalis/efeitos dos fármacos , Ratos Wistar , Staphylococcus aureus/efeitos dos fármacos , Dente/efeitos dos fármacos , Dente/patologia , Óxido de Zinco/química , Óxido de Zinco/uso terapêutico , Óxido de Zinco/toxicidade
12.
Sci Rep ; 11(1): 14017, 2021 07 07.
Artigo em Inglês | MEDLINE | ID: mdl-34234228

RESUMO

Cracked tooth syndrome (CTS) is a common clinical finding for teeth, it affects about 5% of all adults each year. The finding of CTS is favored by several risk factors such as restorations, bruxism, occlusion habits, and age. Treatment options range, depending on the severity, from no treatment at all to tooth extraction. Early diagnosis of CTS is crucial for optimal treatment and symptom reduction. There is no standard procedure for an evidence-based diagnosis up to date. The diagnosis is a challenge by the fact that the symptoms, including pain and sensitivity to temperature stimuli, cannot be clearly linked to the disease. Commonly used visual inspection does not provide in-depth information and is limited by the resolution of human eyes. This can be overcome by magnifying optics or contrast enhancers, but the diagnosis will still strongly rely on the practicians experience. Other methods are symptom reproduction with percussions, thermal pulp tests or bite tests. Dental X-ray radiography, as well as computed tomography, rarely detect cracks as they are limited in resolution. Here, we investigate X-ray dark-field tomography (XDT) for the detection of tooth microcracks. XDT simultaneously detects X-ray small-angle scattering (SAXS) in addition to the attenuation, whereas it is most sensitive to the micrometer regime. Since SAXS originates from gradients in electron density, the signal is sensitive to the sample morphology. Microcracks create manifold interfaces which lead to a strong signal. Therefore, it is possible to detect structural changes originating from subpixel-sized structures without directly resolving them. Together with complementary attenuation information, which visualizes comparatively large cracks, cracks are detected on all length-scales for a whole tooth in a non-destructive way. Hence, this proof-of principle study on three ex-vivo teeth shows the potential of X-ray scattering for evidence-based detection of cracked teeth.


Assuntos
Síndrome de Dente Quebrado/diagnóstico por imagem , Tomografia Computadorizada por Raios X/métodos , Síndrome de Dente Quebrado/patologia , Gerenciamento Clínico , Humanos , Processamento de Imagem Assistida por Computador , Dente/diagnóstico por imagem , Dente/patologia
13.
J Comp Pathol ; 185: 8-17, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-34119235

RESUMO

Histopathological changes in tooth structures in dogs with calculus have not been described. The aim of this study was to evaluate the frequency of various histopathological changes in the dentine of teeth that had been surgically extracted from dogs with calculus. Data including breed, sex, age, reason for tooth extraction and dental history were obtained for each animal. A total of 158 teeth (45 incisors, 31 canines, 35 premolars and 47 molars) with calculus were extracted from 74 dogs of various ages and breeds. The teeth were decalcified, processed in paraffin wax and stained with haematoxylin and eosin for histopathological analysis. Of the 158 analysed teeth, 71 had dentinal changes, including 45 with external resorption cavities, 11 with osteodentine, six with internal resorption cavities, four with tertiary dentine, four with dentinal degeneration or fragmentation, and one with predentine degeneration or fragmentation. Canine teeth were the least commonly affected. Areas of dentinal degeneration or fragmentation unrelated to resorption cavities were only seen in the incisor and molar teeth. Dentinal changes and their frequencies were similar among the incisor, premolar and molar teeth. The presence or extension of dental calculus was not associated with the type or frequency of dentinal changes.


Assuntos
Cálculos Dentários , Dentina/patologia , Doenças do Cão , Dente , Animais , Cálculos Dentários/veterinária , Cães , Dente/patologia
14.
Sci Rep ; 11(1): 12877, 2021 06 18.
Artigo em Inglês | MEDLINE | ID: mdl-34145353

RESUMO

Corrosion patterns induced by gastric fluids on the skeleton of prey animals may depend on the nature of the corrosive agents (acid, enzymes) as well as on the composition of the hard parts and the soft tissues that surround them. We propose a framework for predicting and interpreting corrosion patterns on lizard teeth, our model system, drawing on the different digestive pathways of avian and non-avian vertebrate predators. We propose that high-acid, low-enzyme systems (embodied by mammalian carnivores) will lead to corrosion of the tooth crowns, whereas low-acid, high-enzyme systems (embodied by owls) will lead to corrosion of the tooth shafts. We test our model experimentally using artificial gastric fluids (with HCl and pepsin) and feeding experiments, and phenomenologically using wild-collected owl pellets with lizard remains. Finding an association between the predictions and the experimental results, we then examine corrosion patterns on nearly 900 fossil lizard jaws. Given an appropriate phylogenetic background, our focus on physiological rather than taxonomic classes of predators allows the extension of the approach into Deep Time.


Assuntos
Cárie Dentária/patologia , Fósseis , Lagartos , Dente/patologia , Ração Animal , Animais , Digestão , Modelos Animais de Doenças , Furões , Paleontologia , Dente/anatomia & histologia
15.
J Med Life ; 14(2): 287-294, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34104255

RESUMO

A hopeless tooth from a periodontal point of view, with severe bone resorption, mobility and abnormal tooth migration, is often extracted. In advanced cases, function and esthetics are impaired, and an interdisciplinary treatment is requested. Retaining or not these teeth is based on clinician judgment. A growing body of evidence claims that prognosis has great potential to be improved in a motivated patient with good oral hygiene and regular maintenance. This case report aims to present a periodontal regenerative technique combining enamel matrix protein derivatives and a particulated xenograft to treat intraosseous defects caused by periodontitis. The patient healed uneventfully, and no complications were recorded after the surgical procedure. To correct abnormal tooth migration and improve function and esthetics, orthodontic treatment was instituted. Tooth prognosis improved from hopeless to questionable. This approach extended the life span of a compromised tooth, improving periodontal support and decreasing tooth mobility. This could be an alternative to extraction and implant.


Assuntos
Dente/patologia , Feminino , Seguimentos , Humanos , Pessoa de Meia-Idade , Periodontite/diagnóstico por imagem , Periodontite/patologia , Prognóstico , Suturas , Dente/diagnóstico por imagem , Dente/cirurgia , Extração Dentária
16.
Sci Rep ; 11(1): 12118, 2021 06 09.
Artigo em Inglês | MEDLINE | ID: mdl-34108558

RESUMO

Human identification from burned remains poses a challenge to forensic laboratories, and DNA profiling is widely used for this purpose. Our aim was to evaluate the effect of temperature on DNA degradation in human teeth. Thirty teeth were exposed to temperatures of 100, 200, or 400 °C for 60 min. DNA was quantified by Real-Time qPCR (Quantifiler Human DNA Quantification Kit) and fluorescence spectroscopy (Qubit 3.0 Fluorometer). DNA degradation was evaluated by using STR markers (AmpFLSTR Identifiler Plus PCR Amplification Kit) to determine the allele and locus dropout, inter-locus balance, and degradation slope (observed (Oa) to expected (Ea) locus peak height ratio against the molecular weight). Most of the genomic DNA was degraded between 100 °C and 200 °C. At 100 °C, locus dropout ratios showed significant differences between the largest loci (FGA, D7S820, D18S51, D16S539, D2S1338 and CSF1PO) and amelogenin. Inter-locus balance values significantly differed between all dye channels except between NED and PET. The dropout ratio between D18S51 (NED) and amelogenin (PET) can be recommended for the evaluation of DNA degradation. The Oa/Ea regression model can predict locus peak heights in DNA degradation (R2 = 0.7881). These findings may be useful to assess the reliability of DNA typing for human identification in teeth subjected to prolonged incineration.


Assuntos
DNA/metabolismo , Resposta ao Choque Térmico , Dente/metabolismo , Adulto , Idoso , DNA/análise , DNA/genética , Impressões Digitais de DNA , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase , Dente/patologia , Adulto Jovem
17.
Forensic Sci Int ; 325: 110859, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34098475

RESUMO

The identification of skeletal human remains, severely compromised by putrefaction, or highly deteriorated, is important for legal and humanitarian reasons. There are different tools that can help in the identification process such as anthropological and genetic studies. The success observed during the last decade in genetic analysis of skeletal remains has been possible especially due to the refinements of DNA extraction and posterior analysis techniques. However, despite these progresses, many challenges keep influencing the results of such analysis, mainly the limited amount and the degradation of the DNA recovered from badly preserved samples. By now, there is still no wide-range knowledge about post-mortem kinetics of DNA degradation. Therefore, taphonomy studies can play a key role in the reconstruction of post-mortem transformations that skeletal remains, and consequently DNA, have undergone. Thus, the goal of the present review focuses on the assessment of the literature regarding the possible effect of intrinsic (characteristics of the bone) and extrinsic (environmental) factors on the state of preservation of skeletal remains recovered in a terrestrial environment and their genetic material. The establishment of useful indicators describing the state of the remains is a key factor in order to determine their suitability for posterior biomolecular analysis.


Assuntos
Restos Mortais , Degradação Necrótica do DNA , DNA/análise , Mudanças Depois da Morte , Densidade Óssea , Osso e Ossos/química , Osso e Ossos/patologia , Colágeno/metabolismo , Impressões Digitais de DNA , Medicina Legal , Água Subterrânea , Humanos , Umidade , Concentração de Íons de Hidrogênio , Solo/química , Microbiologia do Solo , Temperatura , Dente/química , Dente/patologia
18.
Sci Prog ; 104(2): 368504211008054, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33827332

RESUMO

Human teeth have become a prominent source of DNA for human forensic identification as their biological structure is highly resistant to extreme conditions. Previous forensic identification was mainly dependent on the pulp and the other hard tissues of intact teeth. However, there is high likelihood that only carious teeth can be available for forensic analysis. This study aimed to validate the use of the carious part of the teeth for forensic identification and to compare two DNA extraction methods-the operative technique with the cervical cut technique for human identification using STR typing. The reliability of STR markers in carious part of the teeth was evaluated in 120 carious teeth (60 dental pulp and 60 dentinal carious tissues, respectively) with considerable coverage of gender type and age range to avoid false exclusions. The study was performed on genuine data set where samples have been extracted by proficient dentist during the treatment operation and collected for further analysis. Complete DNA was extracted and the corresponding human identification profile was obtained using the GoldenEye™DNA ID system 20A kit. The operative technique showed a conservative approach to the sampling of carious tissues and allowed safe access to collect carious tissues, whereas the cervical cut technique permitted access to the root canals and complete sampling of the pulp tissues. The findings indicated that there was no significant association between the cervical cut and operative cut techniques (p = 0.165). In addition, there was no statistically significant association between the various teeth types and the obtained profiles observed. The operative technique, by drilling holes on the defected surface of carious human teeth and gentle hand excavation of carious tissues, was indicated to be very efficient, preserving, time-saving, and cost-effective in the recovery of human DNA from carious teeth. The result gives new insights that the carious tissues of human carious teeth might be as valid as the healthy teeth for forensic human identification.


Assuntos
Cárie Dentária , Dente , DNA , Cárie Dentária/diagnóstico , Cárie Dentária/patologia , Antropologia Forense , Humanos , Reprodutibilidade dos Testes , Dente/patologia
19.
Int J Mol Sci ; 22(6)2021 Mar 16.
Artigo em Inglês | MEDLINE | ID: mdl-33809663

RESUMO

Specific stem cell populations within dental mesenchymal tissues guarantee tooth homeostasis and regeneration throughout life. The decision between renewal and differentiation of stem cells is greatly influenced by interactions with stromal cells and extracellular matrix molecules that form the tissue specific stem cell niches. The Cxcl12 chemokine is a general marker of stromal cells and plays fundamental roles in the maintenance, mobilization and migration of stem cells. The aim of this study was to exploit Cxcl12-GFP transgenic mice to study the expression patterns of Cxcl12 in putative dental niches of intact and injured teeth. We showed that endothelial and stromal cells expressed Cxcl12 in the dental pulp tissue of both intact molars and incisors. Isolated non-endothelial Cxcl12+ dental pulp cells cultured in different conditions in vitro exhibited expression of both adipogenic and osteogenic markers, thus suggesting that these cells possess multipotent fates. Taken together, our results show that Cxcl12 is widely expressed in intact and injured teeth and highlight its importance as a key component of the various dental mesenchymal stem cell niches.


Assuntos
Quimiocina CXCL12/genética , Regulação da Expressão Gênica , Células-Tronco Mesenquimais/metabolismo , Nicho de Células-Tronco/genética , Traumatismos Dentários/genética , Dente/patologia , Animais , Quimiocina CXCL12/metabolismo , Polpa Dentária/metabolismo , Incisivo/metabolismo , Camundongos Transgênicos , Dente Molar/metabolismo , Receptores CXCR4/metabolismo
20.
Sci Rep ; 11(1): 7913, 2021 04 12.
Artigo em Inglês | MEDLINE | ID: mdl-33846470

RESUMO

The purpose of the retrospective study was to investigate the long-term result of implant-induced injury on the adjacent tooth. The subjects of this retrospective study were patients who had received implants and had tooth injury; direct invasion of root (group I), root surface contact (group II), or < 1 mm distance of the implant from the root (group III). Clinical and pathological changes were periodically examined using radiographs and intra-oral examinations. Paired t-tests and chi-square tests were used to evaluate the implant stability quotient (ISQ) of implant and tooth complications, respectively (α = 0.05). A total of 32 implants and teeth in 28 patients were observed for average 122.7 (± 31.7, minimum 86) months. Seven teeth, three of which were subsequently extracted, needed root canal treatment. Finally, 90.6% of the injured teeth remained functional. Complications were significant and varied according to the group, with group I showing higher events than the others. The ISQs increased significantly. One implant in group I resulted in osseointegration failure. The implant survival rate was 96.9%. In conclusion, it was found even when a tooth is injured by an implant, immediate extraction is unnecessary, and the osseointegration of the invading implant is also predictable.


Assuntos
Implantes Dentários/efeitos adversos , Traumatismos Dentários/etiologia , Dente/patologia , Endodontia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Dente/diagnóstico por imagem , Traumatismos Dentários/diagnóstico por imagem
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...