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1.
Acta Odontol Latinoam ; 33(1): 27-32, 2020 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-32621596

RESUMO

The aim of this study was to assess the influence of different pre-treatment approaches on glass ionomer cement (GIC) bond strength (BS) to dentine. Sixty bovine incisors were allocated into six groups according to substrate - sound or caries-affected dentine; and substrate pre-treatment approach - no conditioning (control), polyacrylic acid for 10 s and phosphoric acid for 7 s. Teeth in the caries-affected dentine group were previously submitted to cariogenic pH-cycling challenge. After dentine pre-treatment, according to experimental groups, polyethylene tubes were placed on flat dentine surfaces and filled with GIC. Teeth were stored in distilled water for 24 h at 37 °C and then submitted to microshear test (0.5 mm/min). Failure pattern analysis was performed under stereomicroscope (400x). Data were analysed using two-way ANOVA and Tukey's test (α=5%). Statistically significant differences were found for the pre-treatment approach, regardless of substrate (p<0.001). The polyacrylic acid group and control group had similar BS values, and were both better than the phosphoric acid group. In general, GIC had better bonding performance in sound dentine than in caries-affected dentine. In conclusion, dentine pre-treatment with polyacrylic acid did not improve the performance of GIC restoration on clinically relevant substrates.


Assuntos
Resinas Acrílicas/farmacologia , Colagem Dentária/métodos , Dentina/efeitos dos fármacos , Cimentos de Ionômeros de Vidro/química , Animais , Bovinos , Infiltração Dentária/prevenção & controle , Dentina/diagnóstico por imagem , Dentina/patologia , Teste de Materiais
2.
Int J Nanomedicine ; 15: 2829-2839, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32368057

RESUMO

Objective: To investigate the remineralizing and staining effects of sodium fluoride (NaF) solution with polyethylene glycol-coated silver nanoparticles (PEG-AgNPs) on artificial dentine caries. Materials and Methods: Demineralized human dentine blocks were allocated to three groups. The blocks in group 1 underwent a topical application of a 12% silver diamine fluoride (SDF, 14,150 ppm fluoride) solution. The blocks in group 2 received a topical application of a 2.5% NaF (11,310 ppm fluoride) with PEG-AgNPs (400 ppm silver). The blocks in group 3 received deionized water. All blocks were subjected to pH cycling for 8 days. The surface morphology and cross-sectional features were investigated using scanning electron microscopy (SEM). The color parameters, crystal characteristics, lesion depth, and collagen degradation of the blocks were assessed using digital spectrophotometry, X-ray diffraction (XRD), micro-computed tomography, and spectrophotometry with a hydroxyproline assay, respectively. Results: The SEM showed that dentine collagen was exposed in group 3 but not in groups 1 and 2. The mean lesion depths in groups 1 to 3 were 118±7 µm, 121±14 µm, and 339±20 µm, respectively (groups1,2<3; p<0.001). The data indicated that fluoridated PEG-AgNPs introduced no significant color effect on dentine, but SDF caused distinct discoloration. The XRD indicated that silver chloride was formed in group 1, and fluorapatite was detected in groups 1 and 2. The concentration of hydroxyproline liberated from collagen was significantly less in groups 1 and 2 than in group 3. Conclusion: The use of NaF solution with PEG-AgNPs can remineralize artificial dentine caries and inhibit collagen degradation without causing significant tooth staining.


Assuntos
Cárie Dentária/tratamento farmacológico , Dentina/efeitos dos fármacos , Nanopartículas Metálicas/uso terapêutico , Fluoreto de Sódio/farmacologia , Remineralização Dentária/métodos , Colágeno , Cor , Dentina/metabolismo , Dentina/patologia , Fluoretos/farmacologia , Fluoretos Tópicos/química , Fluoretos Tópicos/farmacologia , Humanos , Hidroxiprolina/metabolismo , Nanopartículas Metálicas/administração & dosagem , Nanopartículas Metálicas/química , Microscopia Eletrônica de Varredura , Dente Serotino/efeitos dos fármacos , Dente Serotino/patologia , Dente Serotino/ultraestrutura , Polietilenoglicóis/química , Compostos de Amônio Quaternário/química , Compostos de Amônio Quaternário/farmacologia , Prata , Compostos de Prata/química , Compostos de Prata/farmacologia , Fluoreto de Sódio/administração & dosagem , Difração de Raios X , Microtomografia por Raio-X
3.
Zhonghua Kou Qiang Yi Xue Za Zhi ; 55(5): 316-322, 2020 May 09.
Artigo em Chinês | MEDLINE | ID: mdl-32392973

RESUMO

Developmental disorders of dental hard tissues are important components of non-carious diseases, which mainly include amelogenesis imperfecta and hereditary dentin disorders with various subtypes. In the absence of effective intervention, these disorders would lead to tooth sensitivity, defects of tooth structure or even loss of tooth, affecting the masticatory function and facial aesthetic configuration. At present, many dental clinicians may not have sufficient understanding of the diseases, and it is urgent to pay attention to the diseases per se and the patients affected. Based on the summary of the current research progresses, this article focuses on the clinical classification, the disease phenotype and the pathogenesis of gene mutations, in order to provide reference and help for the dental clinicians as well as the patients.


Assuntos
Mutação , Doenças Dentárias/genética , Dente/patologia , Amelogênese Imperfeita , Dentina/patologia , Humanos
4.
Lasers Med Sci ; 35(7): 1621-1628, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32333336

RESUMO

The aim of this study was to compare the clinical performance of clinical-visual examination using the International Caries Detection and Assessment System (ICDAS) II, digital bitewing radiography, near-infrared light transillumination (NIR-LT), and laser fluorescence (LF) for the detection of non-cavitated proximal enamel and dentin caries. The study included 335 patients, aged 12-18 years, with no cavities in the posterior teeth. Clinical-visual inspections of 335 non-cavitated proximal caries were performed by two examiners. For enamel caries, clinical validation included a combination of clinical-visual and digital bitewing radiography assessments. For dentin caries, the clinical validation was opening the cavity. The accuracy rate, sensitivity, specificity, predictive values, and areas under receiver operating characteristic curves were determined. The agreement between the examiners' measurements was calculated using the kappa coefficient. The sensitivity, specificity, and accuracy of the methods were compared using the McNemar test. The significance level was set at p < 0.05. Digital bitewing radiography had the highest sensitivity (0.96) and accuracy (0.96), and LF had the lowest sensitivity (0.38) and accuracy (0.39). After separation of the lesions into enamel and dentin caries, clinical-visual examination had the highest sensitivity (0.98) and accuracy (0.98) for enamel caries, while digital bitewing radiography had the highest sensitivity (0.97) and accuracy (0.97) for dentin caries. The NIR-LT method had a higher sensitivity for enamel caries (0.86). Each method also differed significantly from the others (p < 0.001). Digital bitewing radiography gave the best prediction of proximal enamel and dentin caries. NIR-LT showed good potential for detection of proximal caries.


Assuntos
Cárie Dentária/diagnóstico por imagem , Cárie Dentária/diagnóstico , Esmalte Dentário/efeitos da radiação , Dentina/efeitos da radiação , Raios Infravermelhos , Lasers , Radiografia Interproximal , Transiluminação , Cárie Dentária/patologia , Esmalte Dentário/diagnóstico por imagem , Dentina/diagnóstico por imagem , Dentina/patologia , Fluorescência , Humanos , Valor Preditivo dos Testes , Curva ROC , Reprodutibilidade dos Testes , Adulto Jovem
5.
Vet J ; 255: 105421, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31982081

RESUMO

Fissures on the occlusal surface of equine cheek teeth are commonly encountered during oral examination. Generally, their presence is considered abnormal but their aetiopathogenesis and clinical impact on pulp disease is still undetermined. The aims of this research were to study the extent of occlusal cheek teeth fissures using high-resolution X-ray computed tomography (µCT) imaging and their histological characteristics. Twenty-seven teeth (of 15 horses) were scanned using µCT imaging to analyse fissure extent. Histological examination of a subset of teeth (n=7) was performed. Forty-three type 1 fissures (35 type 1a, eight type 1b) were identified. The mean length of the fissure of type 1a and type 1b on the occlusal surface was 3.47+/-1.60mm and 13.64+/-7.40mm, respectively. Their mean depth was 13.22+/-10.76mm and 7.42+/-6.42mm, respectively. Potential risk factors associated with fissure depth were identified using a multivariable mixed model The location of the fissure and Triadan number were significantly associated with fissure depth. Fissures could be identified on histological sections with the presence of organic material inside the fissure, microorganisms in the continuation of the fissure extending into the dentinal tubules and the presence of reactionary dentine. This study suggests that fissures can provide a pathway for microorganisms to enter the dentinal tubules, potentially resulting in local pulpal inflammation. It appears that in healthy teeth, vital odontoblasts react by producing reactionary (tertiary) dentin, which lacks patent tubules and thus provides a proper seal.


Assuntos
Fissuras Dentárias/veterinária , Doenças dos Cavalos/patologia , Dente Molar/patologia , Animais , Fissuras Dentárias/diagnóstico por imagem , Fissuras Dentárias/epidemiologia , Fissuras Dentárias/microbiologia , Dentina/patologia , Doenças dos Cavalos/epidemiologia , Cavalos , Tomografia Computadorizada por Raios X/veterinária
6.
J Endod ; 46(2): 252-257, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31831180

RESUMO

INTRODUCTION: This study evaluated 4 different light-emitting diode (LED) transilluminators and the impact of operator experience in the detection of dentinal defects through an ex vivo TRUEJAW surgical model (Dental Engineering Laboratories, Santa Barbara, CA). METHODS: Forty-four extracted and endodontically treated mandibular premolar teeth were evaluated. Teeth were mounted in the models followed by surgical flaps and osteotomies to expose the apical third of the roots. After apical resection, the root-end surfaces were randomly inspected for the presence or absence of dentinal defects using a dental operating microscope (DOM) at ×19.4 magnification by experienced and novice LED evaluators. The assessment was made with the DOM light and 4 masked LED transilluminators of different diameters and luminous flux. The teeth were examined outside the models to establish the ground truth. The sensitivity, specificity, and kappa and McNemar test values of each light source by examiner were calculated. RESULTS: The use of LED transilluminators improved the diagnostic sensitivity of dentinal defects when compared with the DOM light alone for both examiners. For the LED-experienced evaluator, the medium-low transilluminator had statistically significant higher sensitivity than the DOM light and the small-low and small-high transilluminators (P < .05). For the novice LED evaluator, the medium-high transilluminator had the highest sensitivity. There was a statistically significant difference between the sensitivities of the medium-low transilluminator between the examiners (P < .05). CONCLUSIONS: Within the limitations of this ex vivo surgical study, dentinal defects were more often detected with the LED transilluminators with a larger diameter and increased lumens. The operator's LED transilluminator experience was found to have a positive effect on the detection of dentinal defects using transillumination.


Assuntos
Dentina , Microcirurgia , Transiluminação , Dente Pré-Molar , Equipamentos Odontológicos , Dentina/patologia , Humanos
7.
Acta Odontol Scand ; 78(1): 6-12, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31267814

RESUMO

Aim: To describe the distribution of enamel-, dentin-, root- and secondary caries within an elderly population in Northern-Norway.Material and methods: A study population (n = 1 173) was divided into age groups: 65-69 (seniors), 70-74 (young elderly), 75-79 (elderly) and 80-94 (old elderly). Seven examiners registered presence, location and severity of caries lesions on x-rays and intra-oral photos. Severity of approximal, occlusal, secondary and root caries was graded (D1-2: into enamel; D3-5: into dentin, root caries), and decayed missing filled surfaces/teeth (DMFS/DMFT) were calculated. T-test, ANOVA and a Bonferroni correction were used.Results: The seniors had more primary caries (DS1-5) compared to the oldest age groups, while the old elderly had significantly more secondary caries than the other groups (p < .05). Of those ≥65 years 13.8% were affected with root caries, 21% among the oldest. About 48% of the old elderly had one or more surfaces with untreated caries lesions into dentin, while for the other groups the number was 35% (p < 0.05).Conclusion: Individuals seem to maintain good oral health up to at least 80 years of age. Those older than 80 years have more root caries needing more intensified caries-controlling measures and a higher prevalence of untreated dentin lesions often in need of operative treatment.


Assuntos
Esmalte Dentário , Idoso , Idoso de 80 Anos ou mais , Índice CPO , Cárie Dentária/epidemiologia , Esmalte Dentário/patologia , Dentina/patologia , Feminino , Humanos , Masculino , Noruega/epidemiologia , Prevalência
8.
Niger J Clin Pract ; 22(11): 1576-1582, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31719280

RESUMO

Aims: To evaluate the diagnostic abilities of near-infrared light transillumination (using the DIAGNOcam) and bitewing radiographs in detecting cavitated proximal carious lesions in primary molars. Subjects and Methods: The study was a cross-sectional analytical, clinical study. The proximal surfaces of primary molars of healthy 5- to 8-year-old children were radiographically screened for the presence of carious lesions in the enamel or outer third of dentin (D1). Two trained and calibrated examiners evaluated the depth of caries in bitewing radiographs and DIAGNOcam images and then verified the presence of cavitation by direct visual examination using the "International Caries Detection and Assessment System" after temporary tooth separation. Results: A total of 236 proximal lesions were included in the study. Most of the clinically cavitated lesions (51.9%) were D1 radiographically and in outer dentin lesions (scores 3 and 4) by the DIAGNOcam (37% and 48.1%, respectively). Although DIAGNOcam showed higher sensitivity (0.852) compared to the radiographs (0.519), it showed slightly less specificity (0.569) compared to the radiographs (0.579). However, DIAGNOcam showed higher value of the area under the curve (AUC = 0.722; P < 0.001) compared to the radiographic method (AUC = 0.561; P = 0.308). Conclusions: The DIAGNOcam showed higher sensitivity and better accuracy than bitewing radiographs in diagnosing cavitated proximal lesions in primary molars and can be generally considered as an alternative to radiographs to detect cavitation without the hazards of ionizing radiation in children.


Assuntos
Cárie Dentária/diagnóstico , Microrradiografia/instrumentação , Microrradiografia/métodos , Radiografia Interproximal/métodos , Radiografia Dentária/métodos , Dente Decíduo/diagnóstico por imagem , Transiluminação , Criança , Pré-Escolar , Estudos Transversais , Cárie Dentária/patologia , Esmalte Dentário/diagnóstico por imagem , Esmalte Dentário/patologia , Dentina/diagnóstico por imagem , Dentina/patologia , Feminino , Humanos , Masculino , Dente Molar/patologia , Radiografia Dentária Digital , Reprodutibilidade dos Testes , Sensibilidade e Especificidade
9.
Acta Odontol Latinoam ; 32(2): 75-78, 2019 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-31664297

RESUMO

The aim of the present study was to identify the characteristics and severity of tooth wear in children aged 2 to 5 years attending a kindergarten in the city of Medellin. The study population comprised 92 children aged 2 to 5 years attending a kindergarten in Medellin; the final sample included 86 children selected using a non-probabilistic sampling method. All assessments were performed using the index devised by Hansson and Nilner (1989). Prevalence of tooth wear in the studied population was 100%. The observed results confirm previous findings showing that the severity of tooth wear is proportionally related with increase in age, and is mainly due to the physiological wear of teeth. No significant differences in tooth wear facets were observed among the different types of teeth (incisors, canines, molars). It is necessary to develop validated, precise, and comparable indices that allow establishing the etiology and orienting treatment of non-physiological tooth wear.


Assuntos
Dentição Permanente , Erosão Dentária/epidemiologia , Desgaste dos Dentes/epidemiologia , Dente Decíduo , Pré-Escolar , Estudos Transversais , Dentina/patologia , Feminino , Humanos , Incisivo , Masculino , Dente Molar , Prevalência , Inquéritos e Questionários , Erosão Dentária/etiologia
10.
J Pak Med Assoc ; 69(10): 1509-1513, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31622307

RESUMO

Dentine erosion is an increasingly recognised problem, especially in aging population, and various methods have been utilised for its assessment. This narrative review was planned to summarise the methods for the assessment of the early stages of dentine erosion. Relevant original articles published in the English language from 2013 to 2017 were reviewed. Laboratory techniques and methods with in vivo potential were separately studied. It is evident that the assessment of early dentine erosion is complex and requires a combination of methods. For clinical evaluation, chemical analysis and optical methods show great potential but are in need of more validation.


Assuntos
Dentina/diagnóstico por imagem , Erosão Dentária/diagnóstico , Cálcio/análise , Dentina/química , Dentina/patologia , Testes de Dureza , Humanos , Microrradiografia , Microscopia de Força Atômica , Fosfatos/análise , Fluorescência Quantitativa Induzida por Luz , Tomografia de Coerência Óptica , Erosão Dentária/diagnóstico por imagem , Erosão Dentária/patologia
11.
Clin Oral Investig ; 23(10): 3691-3703, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31444695

RESUMO

OBJECTIVES: To define an expert Delphi consensus on when to intervene in the caries process and on existing carious lesions using non- or micro-invasive, invasive/restorative or mixed interventions. METHODS: Non-systematic literature synthesis, expert Delphi consensus process and expert panel conference. RESULTS: Carious lesion activity, cavitation and cleansability determine intervention thresholds. Inactive lesions do not require treatment (in some cases, restorations will be placed for reasons of form, function and aesthetics); active lesions do. Non-cavitated carious lesions should be managed non- or micro-invasively, as should most cavitated carious lesions which are cleansable. Cavitated lesions which are not cleansable usually require invasive/restorative management, to restore form, function and aesthetics. In specific circumstances, mixed interventions may be applicable. On occlusal surfaces, cavitated lesions confined to enamel and non-cavitated lesions radiographically extending deep into dentine (middle or inner dentine third, D2/3) may be exceptions to that rule. On proximal surfaces, cavitation is hard to assess visually or by using tactile methods. Hence, radiographic lesion depth is used to determine the likelihood of cavitation. Most lesions radiographically extending into the middle or inner third of the dentine (D2/3) can be assumed to be cavitated, while those restricted to the enamel (E1/2) are not cavitated. For lesions radiographically extending into the outer third of the dentine (D1), cavitation is unlikely, and these lesions should be managed as if they were non-cavitated unless otherwise indicated. Individual decisions should consider factors modifying these thresholds. CONCLUSIONS: Comprehensive diagnostics are the basis for systematic decision-making on when to intervene in the caries process and on existing carious lesions. CLINICAL RELEVANCE: Carious lesion activity, cavitation and cleansability determine intervention thresholds. Invasive treatments should be applied restrictively and with these factors in mind.


Assuntos
Cárie Dentária/terapia , Esmalte Dentário/patologia , Dentina/patologia , Consenso , Técnica Delfos , Estética Dentária , Humanos
12.
Stem Cell Res ; 38: 101457, 2019 07.
Artigo em Inglês | MEDLINE | ID: mdl-31082676

RESUMO

In our previous work, we established an in vivo coronal pulp regeneration model in which biodegradable hydrogel-made scaffolds carrying rat bone marrow mesenchymal stem cells (BM-MSCs) were implanted in the coronal pulp chamber of pulpotomized rat maxillary first molars. In this study, we investigated the in vivo fate of LacZ-labeled BM-MSCs in our coronal pulp regeneration model. BM-MSCs were nucleofected with pVectOZ-LacZ plasmid encoding ß-galactosidase 1 day before implantation, and the LacZ-transfected BM-MSCs were implanted into the pulpotomized pulp chamber with biodegradable preformed scaffold-hydrogel constructs. Empty vector was used as a control. After 3 and 14 days, the molars were retrieved and subjected to ß-galactosidase staining. At 3 days, ß-galactosidase-expressing cells with a round profile were located mainly around the scaffold. At 14 days, when the pulp-like tissue had been generated, the majority of ß-galactosidase-expressing cells were detected under the newly formed dentin bridge-like structure, where nestin-expressing odontoblast-like cells were arranged. Immunoreactivity for dentin sialoprotein, a marker of mature odontoblasts, was strongly detected under the original dentin. No ß-galactosidase staining was observed in the control group. Thus, we demonstrated that BM-MSCs survived for 2 weeks after implantation and colonized within the site of potential cytodifferentiation. Our findings indicated that BM-MSCs could differentiate into cells involved in mineralized tissue formation in the functionally relevant region.


Assuntos
Células da Medula Óssea/metabolismo , Polpa Dentária/fisiologia , Dentina/fisiologia , Transplante de Células-Tronco Mesenquimais , Células-Tronco Mesenquimais/metabolismo , Dente Molar/fisiologia , Regeneração , Aloenxertos , Animais , Células da Medula Óssea/patologia , Polpa Dentária/patologia , Dentina/patologia , Feminino , Hidrogéis/química , Células-Tronco Mesenquimais/patologia , Ratos , Ratos Endogâmicos F344 , Tecidos Suporte/química
13.
J Endod ; 45(4): 442-446, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30827768

RESUMO

INTRODUCTION: The aim of this study was to investigate the influence of various apical preparation designs for surgical endodontics on stress concentrations in the mesial root of the mandibular molar under different experimental conditions using finite element analysis. METHODS: We designed 2 apical preparation groups according to whether an isthmus was present or not. Each group contained 4 subgroups according to the size of the apical preparation. We constrained the displacement of all nodes at the base of the supporting bone and applied a force of 150 N to the vertical axis. We analyzed stress generation and concentrations numerically for the groups and subgroups. RESULTS: In the subgroups, the von Mises and maximum principal stresses reduced gradually according to the enlargement of the prepared cavity. However, when the preparation extended excessively in the isthmus preparation groups, the situation reversed (ie, both von Mises and maximum principal stresses increased). CONCLUSIONS: Within the limitations of this study, the apical preparation design influenced the distribution of stress concentration. Unlike the overall pattern in which stress decreased as the amount of apical preparation increased, stress increased when the amount of residual dentin was extremely thin.


Assuntos
Preparo da Cavidade Dentária/métodos , Análise do Estresse Dentário , Endodontia , Análise de Elementos Finitos , Mandíbula , Dente Molar , Estresse Mecânico , Raiz Dentária , Modelos Dentários , Dentina/anatomia & histologia , Dentina/patologia , Dentina/cirurgia , Humanos , Raiz Dentária/anatomia & histologia , Raiz Dentária/patologia , Raiz Dentária/cirurgia
14.
Microsc Res Tech ; 82(7): 1114-1126, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30907056

RESUMO

Microenergy dispersive X-ray fluorescence (µ-EDXRF) spectroscopy and scanning electron microscopy (SEM) were used to test the hypothesis that zirconia modified glass ionomer cement (GIC) could improve resistance to erosion-abrasion to a greater extent than conventional cement. Bovine enamel (n = 40) and dentin (n = 40) samples were prepared with cavities, filled with one of the two restorative materials (GIC: glass-ionomer cement or ZrGIC: zirconia-modified GIC). Furthermore, the samples were treated with abrasion-saliva (AS) or abrasion-erosion cycles (AE). Erosive cycles (immersion in orange juice, three times/day for a duration of 1 min over a 5 day period) and/or abrasive challenges (electric toothbrush, three times/day for a duration of 1 min over a 5 day period) were performed. Positive mineral variation (MV%) on the enamel after erosion-abrasion was observed for both materials (p < 0.05), whereas a negative MV% on the dentin was observed for both materials and treatments (p < 0.05). The SEM images showed clear enamel loss after erosion-abrasion treatment and material degradation was greater in GIC_AE compared to those of the other groups. Toothbrush abrasion showed a synergistic effect with erosion on substance loss of bovine enamel, dentin, GIC, and ZrGIC restorations. Zirconia addition to the GIC powder improved the resistance to abrasive-erosive processes. The ZrGIC materials may find application as a restorative material due to improved resistance as well as in temporary restorations and fissure sealants.


Assuntos
Esmalte Dentário/efeitos dos fármacos , Dentina/efeitos dos fármacos , Cimentos de Ionômeros de Vidro/química , Teste de Materiais , Zircônio/química , Animais , Bovinos , Cárie Dentária , Dentina/patologia , Microscopia Eletrônica de Varredura , Saliva , Espectrometria por Raios X , Erosão Dentária , Escovação Dentária
15.
Am J Phys Anthropol ; 169(1): 122-131, 2019 05.
Artigo em Inglês | MEDLINE | ID: mdl-30882907

RESUMO

OBJECTIVES: This study investigates vitamin D deficiency patterns in individuals from birth to the beginning of adolescence. Microscopic computed tomography (micro-CT) evaluation of interglobular dentine (IGD) in teeth provides information on the age of disease onset and the number of deficient periods per individual, which will increase our understanding of factors influencing vitamin D deficiency prevalence, including sociocultural practices and latitude. MATERIALS AND METHODS: Beemster and Hattem, two Dutch 17th-19th century communities, yielded relatively high prevalences of rickets (15-24%) and residual rickets (15-24%). From the affected individuals, a subsample of 20 teeth were selected for micro-CT scanning. Thin sections were made of 17 teeth, consisting of 6 teeth with and 11 teeth without observable IGD on micro-CT that were included for method comparison. RESULTS: About 19 out of 29 (65.5%) individuals (one tooth was deemed unobservable) presented with IGD on micro-CT. Eight of the 11 (72.7%) individuals without IGD on micro-CT demonstrated histologically visible IGD. In 40.7% (11/27) of the affected individuals (combined micro-CT and histology results), vitamin D deficiency was recurrent, and in four individuals, some episodes occurred at approximately annual intervals suggesting vitamin D deficiency was seasonal. In three individuals, IGD occurred in the dentine formed around birth, suggesting maternal vitamin D deficiency. DISCUSSION: Micro-CT analysis of IGD is found to be a valuable non-destructive method that can improve our understanding of the influence of sociocultural practices and latitude on disease development within age and sex groups in past communities.


Assuntos
Dentina/patologia , Raquitismo/etnologia , Raquitismo/patologia , Desmineralização do Dente/patologia , Adolescente , Adulto , Antropologia Física , Criança , Pré-Escolar , Dentina/diagnóstico por imagem , Feminino , História do Século XVII , História do Século XVIII , História do Século XIX , Humanos , Lactente , Masculino , Pessoa de Meia-Idade , Países Baixos/etnologia , Raquitismo/diagnóstico por imagem , Raquitismo/história , Estações do Ano , Microtomografia por Raio-X , Adulto Jovem
16.
Sensors (Basel) ; 19(4)2019 Feb 20.
Artigo em Inglês | MEDLINE | ID: mdl-30791530

RESUMO

The mineral content of dental hard tissues has traditionally been measured by destructive tests such as transverse microradiography. Microfocus X-ray computed tomography (micro CT) has enabled non-destructive 3D assessment of tooth demineralization. This study compared the preventive effects of silver diammine fluoride (SDF) and potassium iodide (KI) in comparison with fluoride varnish. SDF has been known to arrest caries but darkens the tooth. KI creates a precipitate with SDF that reduces the discoloration, but its effects on SDF efficacy in terms of preventing demineralization of at-risk root dentin surfaces is unknown. Bovine root dentin blocks were randomly distributed into four groups and subjected to a pretreatment in each group (n = 8); Control: deionized water (DIW); F-Varnish: 5% sodium fluoride varnish: 38% Saforide; SDF+KI: SDF followed by saturated solution of KI in DIW. The treated dentin was subjected to 8 cycles of demineralization (pH 5) for 14 h and remineralization in artificial saliva (pH 7) for 10 h. Specimens were then scanned for 12 min using micro CT at 73 KV and 1012 µA with 8.3 µm resolution. The 3D images were analyzed in Amira software to calculate lesion depth (LD), surface layer mineral density (SL) and mineral loss (ΔZ) for each specimen. One-way ANOVA with Bonferroni posthoc showed that there was a statistically significant difference between Control and all three other groups for all parameters (P < 0.001), however, there was no statistical difference among F-Varnish, SDF and SDF+KI (P > 0.05). Single application of F-Varnish, SDF and SDF+KI showed comparable preventive effects against root dentin demineralization. Application of KI did not affect anti-demineralization properties of SDF in this study. Micro CT is a quick and effective method for objective and high-resolution characterization of dentin caries lesions.


Assuntos
Cárie Dentária/prevenção & controle , Fluoretos Tópicos/administração & dosagem , Iodeto de Potássio/administração & dosagem , Cárie Radicular/prevenção & controle , Animais , Bovinos , Cárie Dentária/diagnóstico por imagem , Cárie Dentária/patologia , Dentina/diagnóstico por imagem , Dentina/efeitos dos fármacos , Dentina/patologia , Humanos , Cárie Radicular/diagnóstico por imagem , Cárie Radicular/patologia , Desmineralização do Dente/diagnóstico por imagem , Desmineralização do Dente/tratamento farmacológico , Raiz Dentária/diagnóstico por imagem , Raiz Dentária/efeitos dos fármacos , Raiz Dentária/patologia , Microtomografia por Raio-X
17.
Am J Phys Anthropol ; 168(4): 764-788, 2019 04.
Artigo em Inglês | MEDLINE | ID: mdl-30771253

RESUMO

OBJECTIVES: To compare relative response of enamel, dentin and bone to developmental stressors between attritional and catastrophic mortality assemblages of pigs. MATERIALS AND METHODS: Heads from 70 Sus scrofa of known sex, weight and age comprising an attritional sample of 50 sick pen (SP) pigs that died prematurely versus 20 control pigs slaughtered at 6 months (Catastrophic assemblage). Hard tissue changes (alveolar bone thinning), abnormal bone formation (Harris lines) and re-modeling (auditory bullae) were recorded. Areas and volumes of coronal enamel and dentin were recorded from microCT scans with Avizo 6.3 and Geomagic Wrap. RESULTS: Attritional and catastrophic assemblages are metrically indistinguishable. Ages at death and tissue measures in the SP pigs are differentially distributed, necessitating partition into developmental outcome cohorts. SP "late death" pigs are of lesser physiological maturity than expected, free of disease, with large dental tissue dimensions, comparable to "Controls". SP "early death" pigs have 5% less dentin and enamel and chronic bone infection. Older cohorts of the SP "early deaths" mortality assemblage show progressively reduced enamel. SP pigs show dental evidence of reduced bone mass in the maxilla. DISCUSSION: Bone, dentin and enamel tissues, each, respond distinctively to developmental stressors. Bone mass evinces malnutrition not disease. Both dental tissue reduction and abnormal bone formation link to chronic infection. Paradoxically, reduced dentin mass signals lower survivorship while reduced enamel signals enhanced survivorship. Meaningful comparison of Attritional and Catastrophic assemblages necessitates recognition of developmental outcome cohorts, stratified by age at death and physiological maturity, to reveal heterogeneity of survivorship, tissue measures and lesions.


Assuntos
Esmalte Dentário , Dentina , Estresse Fisiológico/fisiologia , Doenças Dentárias , Animais , Esmalte Dentário/crescimento & desenvolvimento , Esmalte Dentário/patologia , Dentina/crescimento & desenvolvimento , Dentina/patologia , Feminino , Masculino , Sus scrofa , Suínos , Dente/crescimento & desenvolvimento , Dente/patologia , Doenças Dentárias/patologia , Doenças Dentárias/veterinária
18.
J Oral Sci ; 61(1): 19-24, 2019 Mar 28.
Artigo em Inglês | MEDLINE | ID: mdl-30726799

RESUMO

Minimally invasive treatment protocols may leave a residual layer of carious dentin, which requires treatment for the inhibition of bacterial growth beneath restorations. We aimed to examine the in vivo effect of silver diammine fluoride (SDF) and SDF + potassium iodide (KI) application on bacteria present in deep carious lesions. We studied the in vivo efficacy in five patients, each of which had five carious lesions. Dentin samples taken before and after treatment were subjected to microbial analyses. Following treatment with SDF, the median colony-forming unit (CFU) counts per mg of dentin reduced from 9 × 105 to 1.6 × 102 (P < 0.05), and following that with SDF + KI, the counts decreased from 2.9 × 105 to 9.2 × 10 (P < 0.05). The use of chlorhexidine gluconate (CHX) reduced CFU counts from 1.1 × 105 to 4.8 × 102 (P < 0.05). In four of the five patients, no CFUs were found on mitis salivarius-bacitracin agar with respect to SDF or SDF + KI application. For CHX, the median CFU count before treatment was 1.6 × 103 and that after treatment was 1.1 × 102. SDF completely inhibited mutans streptococci growth in four of the five patients, while the growth of anaerobes was not completely inhibited.


Assuntos
Amônia/uso terapêutico , Antibacterianos/uso terapêutico , Cárie Dentária/tratamento farmacológico , Cárie Dentária/microbiologia , Dentina/patologia , Fluoretos/uso terapêutico , Compostos de Prata/uso terapêutico , Amônia/química , Contagem de Colônia Microbiana , Fluoretos/química , Humanos , Compostos de Prata/química , Streptococcus mutans/efeitos dos fármacos
19.
Niger J Clin Pract ; 22(1): 117-124, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30666030

RESUMO

Objective: The aim of this in-vivo study was to evaluate the long-term clinical survival of different deep dentin caries treatment options. Materials and Methods: : In total, 391 patients with at least one permanent tooth with clinically diagnosed deep dentin caries were inspected. Two hundred and fourteen patients were examined at recall visits. Inclusion criteria were teeth with deep caries lesions with pulp vitality but absence of spontaneous pain and periapical alterations. The subjects received either stepwise removal (SWR), complete caries removal (CCR), or direct pulp capping (DPC). The radiological and clinical exams were performed after a mean observation time of 62 months. Success was defined as pulp sensitivity to vitality test and absence of periapical lesions as well as a clinical symptom. Data were statistically analyzed using Kaplan-Meier and log-rank (Mantel-Cox) tests (α = 0.05). Results: Of the total 214 patients evaluated, 126 received SWR, 88 received CCR, and 67 received DPC treatment. One hundred and twenty-seven restorations were amalgam and 141 were composite. The mean observation period was 62 months. Survival rates were 85.7%, 90.9%, and 59.7% for SWR, CCR, and DPC, respectively (P = 0.001). Success rates of amalgam restorations (86.6%) were similar to composite restorations (83%), and both were found to be successful (P = 0.401). Conclusion: SWR treatment should be considered to preserve pulp vitality of deep dentin lesions instead of CCR or DPC. Clinical Relevance: SWR method for deep dentin caries management had acceptable results over 5 years.


Assuntos
Cárie Dentária/terapia , Preparo da Cavidade Dentária/métodos , Capeamento da Polpa Dentária/métodos , Restauração Dentária Permanente/métodos , Dentina/patologia , Adulto , Cárie Dentária/diagnóstico por imagem , Cárie Dentária/patologia , Materiais Dentários , Polpa Dentária/diagnóstico por imagem , Polpa Dentária/patologia , Polpa Dentária/fisiologia , Exposição da Polpa Dentária/patologia , Exposição da Polpa Dentária/prevenção & controle , Dentina/diagnóstico por imagem , Dentição Permanente , Feminino , Humanos , Masculino , Tratamento do Canal Radicular , Resultado do Tratamento
20.
Photodiagnosis Photodyn Ther ; 25: 265-270, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30611863

RESUMO

BACKGROUND: The difference in autofluorescence between enamel and dentine layer has prompted recommendations to use the quantitative light-induced fluorescence (QLF) method for quantifying tooth wear (TW). This study investigated the potential of QLF for distinguishing the severity of occlusal TW based on differences in the autofluorescence intensity. METHODS: In total, 106 extracted permanent molars and premolars having suspected wear without pulp exposure were used. The severity of wear was determined by visually examining all teeth using the tooth wear index (TWI) of Smith and Knight. QLF images were captured and converted into 8-bit grayscale images. The difference in the fluorescence intensity (ΔG) was calculated by comparing mean grayscale levels between sound and worn areas. Finally, histological examination was conducted by stereomicroscope to confirm the presence of dentine exposure. RESULTS: 100 teeth were included in the final analysis without six teeth having enamel cracks around worn area. The ΔG values increased with the severity of TW as quantified using conventional TWI codes, and differed significantly between the sound and enamel- and dentine-wear teeth (P < 0.001). The histology indicated that enamel remained on 57 teeth, while 43 teeth had dentine-exposed wear and showed significant differences in ΔG compared with enamel-remained teeth. CONCLUSIONS: The fluorescence intensity differed significantly depending on the presence of dentine exposure. ΔG could be used to distinguish between sound and enamel- and dentine-wear teeth with a significant correlation. These findings indicate that QLF could be useful for determining the severity of TW of occlusal surfaces noninvasively.


Assuntos
Esmalte Dentário/patologia , Dentina/patologia , Fluorescência Quantitativa Induzida por Luz/métodos , Desgaste dos Dentes/diagnóstico , Dente Pré-Molar , Humanos , Dente Molar/patologia
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