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1.
Artigo em Inglês | MEDLINE | ID: mdl-32145640

RESUMO

Wild edible macro fungi Floccularia luteovirens proved to be a valuable source for the identification of novel lead molecules with therapeutic potential. Nevertheless, the chemical constituents of Floccularia luteovirens are rarely reported due to absence of efficient purification methods. In this study, a hydrophilic interaction chromatography directed by on-line HPLC-DPPH assay has been developed and successfully applied for the isolation of free radical inhibitor from the methanolic extract of Floccularia luteovirens. Using a hydrophilic interaction chromatographic column coupled with the HPLC-DPPH assay for screening the potential radical scavengers, the mid-pressure hydrophilic interaction chromatography (HILIC) proved to be more efficient in the pretreatment stage, yielding the fraction rich in free radical scavengers in good yield (5.9% recovery from 130.0 g of fresh F. luteovirens). From highly potent fraction, the target compound was isolated using the Click XION preparative chromatography with 17.2% recovery. The isolated compound was L-(+)-ergothioneine, where the purity (>95%) and antioxidant activity of were confirmed by chromatography and HPLC-DPPH assay, while the structure of this compound was elucidated from HR ESI-MS and NMR data. This method proved to be very efficient for the recognition and isolation of highly polar free radical inhibitors from fungi extracts, and is also applicable for the purification of highly polar compounds from other sources.


Assuntos
Basidiomycota/química , Ergotioneína/isolamento & purificação , Depuradores de Radicais Livres/isolamento & purificação , Radicais Livres/química , Bioensaio , Cromatografia Líquida de Alta Pressão , Ergotioneína/análise , Depuradores de Radicais Livres/análise , Interações Hidrofóbicas e Hidrofílicas , Estrutura Molecular
2.
Food Chem Toxicol ; 135: 110976, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31743742

RESUMO

This paper aimed to establish the optimal conditions for ultrasound-assisted extraction of polyphenols from domestic garlic (Allium sativum L.) using response surface methodology (RSM) and artificial neural network (ANN) approach. A 4-factor-3-level central composite design was used to optimize ultrasound-assisted extraction (UAE) to obtain a maximum yield of target responses. Maximum values of the two output parameters: 19.498 mg GAE/g fresh weight of sample total phenolic content and 1.422 mg RUT/g fresh weight of sample total flavonoid content were obtained under optimum extraction conditions: 13.50 min X1, 59.00 °C X2, 71.00% X3 and 20.00 mL/g X4. Root mean square error for training, validation, and testing were 0.0209, 3.6819 and 1.8341, respectively. The correlation coefficient between experimentally obtained total phenolic content and total flavonoid content and values predicted by ANN were 0.9998 for training, 0.9733 for validation, and 0.9821 for testing, indicating the good predictive ability of the model. The ANN model had a higher prediction efficiency than the RSM model. Hence, RSM can demonstrate the interaction effects of basic inherent UAE parameters on target responses, whereas ANN can reliably model the UAE process with better predictive and estimation capabilities.


Assuntos
Depuradores de Radicais Livres/isolamento & purificação , Alho/química , Redes Neurais de Computação , Polifenóis/isolamento & purificação , Modelos Químicos , Extração em Fase Sólida/métodos , Solventes/química , Ondas Ultrassônicas
3.
Nat Prod Res ; 34(6): 872-875, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30345814

RESUMO

Raspberry, the fruit of Rubus Chingji Hu, is a widely distributed economic staple food in China. It has long been used as a traditional medicine in mainland China to treat kidney enuresis, nocturnal emission and premature ejaculation in clinic. In this paper, six known compounds (1 - 6) were purified from the fruits of Rubus chingji. Their structures were elucidated as (16α)-16,17-dihydroxy-ent-kauran-2-one17-O-ß-D-glucopyranoside (1), (16R) -16,17-dihydroxy-ent-kaurane-2-one (2), 3,3'-di-O-methylellagic acid 4-(5''-acetyl)-α-L-arabinofuranoside (3), quercilicoside A (4), esculetine (5) and ethyl-ß-D-glucoside (6). All the compounds were isolated from Rubus Chingji Hu for the first time. Compounds 3 and 5 shown distinctive free radical scavenging activities in DPPH and FRAP assays. In addition, no cytotoxicity was observed for compounds 3 and 5 against different cancer cells, suggesting that they might be useful as potential antioxidant agents against various reactive oxygen species.[Formula: see text].


Assuntos
Antioxidantes/isolamento & purificação , Frutas/química , Rubus/química , Antioxidantes/química , Antioxidantes/farmacologia , Linhagem Celular Tumoral , China , Ácido Elágico/análogos & derivados , Depuradores de Radicais Livres/química , Depuradores de Radicais Livres/isolamento & purificação , Depuradores de Radicais Livres/farmacologia , Humanos , Extratos Vegetais/química
4.
Nat Prod Res ; 34(7): 943-949, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30760039

RESUMO

Saussurea involucrata, known for the abundant bioactive components, is a precious traditional Chinese medicine. In this study, a novel guaiane sesquiterpenoid glycoside named (1R, 5R, 6R, 7R, 8S, 11S)-11, 13-dihydrodehydrocostuslactone-8-O-6'-2''(E)-butenoyl-ß-D-glucopyranoside (1), together with seven known compounds (2-8) were isolated from the dried aerial part of S. involucrata. Their structures were elucidated by spectroscopic and physico-chemical analyses. The antioxidant and anti-inflammatory activities of compound 1 were investigated. And compound 1 showed weak radical scavenging activity and low inhibitory activity on nitric oxide (NO) production.


Assuntos
Glicosídeos/isolamento & purificação , Medicina Tradicional Chinesa , Saussurea/química , Sesquiterpenos/isolamento & purificação , Anti-Inflamatórios/química , Anti-Inflamatórios/isolamento & purificação , Anti-Inflamatórios/farmacologia , Antioxidantes/química , Antioxidantes/isolamento & purificação , Antioxidantes/farmacologia , Depuradores de Radicais Livres/química , Depuradores de Radicais Livres/isolamento & purificação , Depuradores de Radicais Livres/farmacologia , Glicosídeos/química , Glicosídeos/farmacologia , Óxido Nítrico/antagonistas & inibidores , Extratos Vegetais/química , Sesquiterpenos/química , Sesquiterpenos/farmacologia
5.
J Ethnopharmacol ; 247: 112257, 2020 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-31589968

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Hydrolea zeylanica L. Vahl. (Hydroleaceae) is an aquatic medicinal plant used as leafy vegetable in some parts of India. In south Odisha and Hazaribag district of Jharkhand, India, decoction of leaves is used as household remedy for diabetes. To our knowledge, no prior studies have examined the antidiabetic activity of H. zeylanica to validate its ethnomedicinal claim. PURPOSE: With this aim in mind, we examined the bioactivity of hydroalcohol fraction of leaves of H. zeylanica (HAHZ) in streptozotocin-induced oxidative stress in diabetic rats. MATERIALS AND METHODS: In vitro antidiabetic and free radical scavenging activities of different fractions of H. zeylanica were performed. The most effective bioactive fraction e.g. HAHZ was considered for kinetic studies to understand the mode of inhibition of α-glucosidase and α-amylase. To understand the chemical composition, GC-MS/MS and LC-MS/MS analysis of HAHZ were performed. To find out the molecular mechanism of action of HAHZ, streptozotocin-induced oxidative stress and metabolic changes in diabetic rats were studied. RESULTS: HAHZ demonstrated significantly higher radical scavenging and antidiabetic activities. Kinetic analysis revealed that HAHZ inhibited α-glucosidase competitively, and α-amylase mixed competitively. To understand the chemical composition, GC-MS/MS and LC-MS/MS analysis of HAHZ identified 32 compounds and among which R-limonene (0.52%), perillartine (0.41%), N-formyl-L-lysine (1.49%), limonen-6-ol, pivalate (1.43%), lidocaine (1.70%) and gamolenic acid (2.80%) were reported to have antioxidant and antidiabetic activities. HAHZ-400 mg/kg showed significant (p < 0.001) improvement in serum markers (SGOT, SGPT, ALP, total bilirubin, total protein, triglycerides, total cholesterol, HDL-C, LDL-C) and oxidative markers (MDA, SOD, CAT, GSH) in serum, liver and pancreas at effective dose dependent manner. In histopathological observation, HAHZ-400 mg/kg showed marked improvement in restoring cellular architecture of liver and pancreas. CONCLUSION: In diabetic rats, the improvement in glycemic control mechanism was achieved upon stimulating insulin secretion by R-limonene, perillartine, N-formyl-L-lysine, limonen-6-ol, pivalate, lidocaine and gamolenic acid of HAHZ.


Assuntos
Organismos Aquáticos/química , Diabetes Mellitus Experimental/tratamento farmacológico , Depuradores de Radicais Livres/farmacologia , Hipoglicemiantes/farmacologia , Extratos Vegetais/farmacologia , Solanales/química , Animais , Glicemia/efeitos dos fármacos , Diabetes Mellitus Experimental/sangue , Diabetes Mellitus Experimental/induzido quimicamente , Etnofarmacologia , Feminino , Depuradores de Radicais Livres/isolamento & purificação , Depuradores de Radicais Livres/uso terapêutico , Humanos , Hipoglicemiantes/isolamento & purificação , Hipoglicemiantes/uso terapêutico , Índia , Insulina/metabolismo , Masculino , Estresse Oxidativo/efeitos dos fármacos , Extratos Vegetais/isolamento & purificação , Extratos Vegetais/uso terapêutico , Folhas de Planta/química , Ratos , Estreptozocina/toxicidade
6.
Food Chem Toxicol ; 136: 111050, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31843533

RESUMO

The pickled radish can be kept at room temperature for years without spoilage. 2,6-dihydroxyacetophenone (DHAP), 4-hydroxybenzaldehyde (HBA), and 4-hydroxyphenethyl alcohol (4-HPEA) were first found from the pickled radish. The structures of three phenolic compounds were elucidated by analysis of their nuclear magnetic resonance and high-resolution electro-spray ionization mass spectrometry data. All these phenolic compounds showed good free radical scavenging capacity except HBA. Both DHAP and 4-HPEA also showed high ferric reducing ability. DHAP showed good antimicrobial activity against Escherichia coli, Bacillus subtilis, and Canidia albicans. HBA demonstrated antimicrobial activity against E. coli and C. albicans but not B. subtilis. Based on the results of MTT assay, these compounds did not show cytotoxicity to LO2 cell line. All results indicated the pickled radish had antioxidant and antimicrobial phenolic compounds. To the best of our knowledge, this report is the first to answer partially the question of why pickled foods can be kept at room temperature for years without spoilage based on the evidence of three phenolic compounds.


Assuntos
Antibacterianos/farmacologia , Antifúngicos/farmacologia , Depuradores de Radicais Livres/farmacologia , Fenóis/farmacologia , Raphanus/química , Antibacterianos/isolamento & purificação , Antibacterianos/toxicidade , Antifúngicos/isolamento & purificação , Antifúngicos/toxicidade , Bacillus subtilis/efeitos dos fármacos , Candida albicans/efeitos dos fármacos , Linhagem Celular , Escherichia coli/efeitos dos fármacos , Depuradores de Radicais Livres/isolamento & purificação , Depuradores de Radicais Livres/toxicidade , Humanos , Testes de Sensibilidade Microbiana , Fenóis/isolamento & purificação , Fenóis/toxicidade
7.
Int J Mol Sci ; 20(23)2019 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-31801293

RESUMO

The gastric secretory trefoil factor family (TFF) peptides xP1 and xP4 are the Xenopus laevis orthologs of mammalian TFF1 and TFF2, respectively. The aim of this study was to analyze the molecular forms of xP1 and xP4 in the X. laevis gastric mucosa by FPLC. xP1 mainly occurred in a monomeric low-molecular-mass form and only a minor subset is associated with the mucus fraction. The occurrence of monomeric xP1 is unexpected because of its odd number of cysteine residues. Probably a conserved acidic residue flanking Cys55 allows monomeric secretion. Furthermore, Cys55 is probably post-translationally modified. For the first time, we hypothesize that the free thiol of monomeric xP1-and probably also its mammalian ortholog TFF1-could have a protective scavenger function, e.g., for reactive oxygen/nitrogen species. In contrast, xP4 mainly occurs in a high-molecular-mass form and is non-covalently bound to a mucin similarly as TFF2. In vitro binding studies with radioactively labeled porcine TFF2 even showed binding to X. laevis gastric mucin. Thus, xP4 is expected to bind as a lectin to an evolutionary conserved sugar epitope of the X. laevis ortholog of mucin MUC6 creating a tight mucus barrier. Taken together, xP1 and xP4 appear to have different gastric protective functions.


Assuntos
Proteínas de Anfíbios/química , Depuradores de Radicais Livres/química , Mucosa Gástrica/metabolismo , Substâncias Protetoras/química , Processamento de Proteína Pós-Traducional , Fator Trefoil-1/química , Proteínas de Anfíbios/isolamento & purificação , Proteínas de Anfíbios/metabolismo , Proteínas de Anfíbios/farmacologia , Animais , Depuradores de Radicais Livres/isolamento & purificação , Depuradores de Radicais Livres/metabolismo , Depuradores de Radicais Livres/farmacologia , Peso Molecular , Mucinas/química , Mucinas/metabolismo , Substâncias Protetoras/isolamento & purificação , Substâncias Protetoras/metabolismo , Substâncias Protetoras/farmacologia , Ligação Proteica , Isoformas de Proteínas/química , Isoformas de Proteínas/isolamento & purificação , Isoformas de Proteínas/metabolismo , Isoformas de Proteínas/farmacologia , Espécies Reativas de Nitrogênio/antagonistas & inibidores , Espécies Reativas de Nitrogênio/metabolismo , Espécies Reativas de Oxigênio/antagonistas & inibidores , Espécies Reativas de Oxigênio/metabolismo , Suínos , Fator Trefoil-1/isolamento & purificação , Fator Trefoil-1/metabolismo , Fator Trefoil-1/farmacologia , Xenopus laevis/fisiologia
8.
Recent Pat Drug Deliv Formul ; 13(4): 310-322, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31849292

RESUMO

BACKGROUND: Excessive blue light light-emitting diode (LED) exposure and consequent oxidative stress causes corneal damage and corneal injuries are the major problem arising these days due to excessive use of mobile phone, TV, environment pollution, etc. Objective: In the present investigation, the protectiveness of carboxymethyl Terminalia catappa (CTC) from blue light LED-induced corneal damage was explored. METHODS: For this purpose, Terminalia catappa (TC) was functionalized by carboxymethylation and its structural modification was confirmed by spectral attributes. Further, the CTC protective eye drop formulations (0.025-1%, w/v) were prepared and evaluated for their capability of protection from blue light LEDinduced corneal damage as compared to CTC protective eye gel (1.25-7%, w/v). The findings pointed towards excellent protection of CTC gel formulations as compared to CTC eye drop formulations. In addition, the prepared optimized CTC gel had thixotropic behavior as evident from percentage structural recovery which was 1.75 fold higher than marketed formulation (I-Comfort, HPMC 2%, w/v). The safety and non-toxicity of CTC protective eye drop and gel were confirmed by HET-CAM test. Further, a rat eye model was implemented that mimic blue light light-emitting diode induced corneal damage in day to day life to assess the protective effect of CTC protective eye drop and gel. RESULTS: The order of protectiveness of CTC formulations was found to be CTC protective eye gel (4%, w/v) (no corneal damage)>marketed eye gel (12.34% corneal damage)=CTC protective eye drop (0.75%, w/v) (17.48% corneal damage)> marketed eye drop (51% corneal damage). The mechanism behind the protective effect of CTC eye drop and gel was associated with good free radical scavenging activity and corneal adhesive property of CTC. It is established from the present work that, carboxymethyl Terminalia catappa has protective action against blue light light-emitting diode induced corneal damage.


Assuntos
Lesões da Córnea/prevenção & controle , Polissacarídeos/isolamento & purificação , Polissacarídeos/farmacologia , Terminalia/química , Administração Oftálmica , Animais , Lesões da Córnea/etiologia , Relação Dose-Resposta a Droga , Depuradores de Radicais Livres/química , Depuradores de Radicais Livres/isolamento & purificação , Depuradores de Radicais Livres/farmacologia , Luz/efeitos adversos , Soluções Oftálmicas , Estresse Oxidativo/efeitos dos fármacos , Polissacarídeos/química , Substâncias Protetoras/química , Substâncias Protetoras/isolamento & purificação , Substâncias Protetoras/farmacologia , Ratos
9.
Fitoterapia ; 139: 104389, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31669963

RESUMO

A new heterodimer, rynchopeterine F (1), a new natural product, rynchopeterine G (2), and eleven known phenolics were isolated from Blap rynchopetera Fairmaire, a kind of medicinal insect utilized by the Yi and Bai Nationality in Yunnan Province of China. Their structures were established on the basis of extensive spectroscopic analyses (1D and 2D NMR, HR-MS) along with calculated electronic circular dichroism method. Rynchopeterine F was a unusual heterodimer of a 3,4-dihudroxy phenylethanol unit fused to a 3,4-dihudroxy phenylacetyl group through two ester bonds with lactic acid, and rynchopeterine G was a 3,4-dihudroxy phenylethanyl monoester succinate. Attributed to the adjacent dihydroxyl grops, compounds 1 and 2 exhibited significant anti-radical activity with an IC50 value of 3.52 and 7.83 µg/mL for DPPH radical-scavenging, similar with that of the positive controls, vitamin C, 6.92 µg/mL and rutin, 8.28 µg/mL.


Assuntos
Besouros/química , Depuradores de Radicais Livres/farmacologia , Fenóis/farmacologia , Animais , China , Depuradores de Radicais Livres/isolamento & purificação , Ácido Láctico/química , Estrutura Molecular , Fenóis/isolamento & purificação , Álcool Feniletílico/química
10.
Fitoterapia ; 139: 104369, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31626911

RESUMO

Five new polyketides, colletotric B (2), 3-hydroxy-5-methoxy-2,4,6-trimethylbenzoic acid (3), colletotric C (4), chaetochromone D (6) and 8-hydroxy-pregaliellalactone B (9), together with four known analogues (1, 5 and 7-8) were isolated from the mangrove endophytic fungus Phoma sp. SYSU-SK-7. Their structures were elucidated by analysis of extensive spectroscopic data and mass spectrometric data. Compounds 1-2 showed strong antimicrobial activity against the P. aeruginosa, MRSA and C. albicans with the MIC values in the range of 1.67-6.28 µg/ml. Furthermore, Compounds 1-5 also exhibited significant α-glucosidase inhibitory activity with the IC50 values in the range of 36.2-90.6 µM. Compound 7 was found to inhibited radical scavenging activity against DPPH with the EC50 value of 11.8 µM.


Assuntos
Anti-Infecciosos/farmacologia , Ascomicetos/química , Depuradores de Radicais Livres/farmacologia , Inibidores de Glicosídeo Hidrolases/farmacologia , Policetídeos/farmacologia , Rhizophoraceae/microbiologia , Anti-Infecciosos/isolamento & purificação , China , Endófitos/química , Depuradores de Radicais Livres/isolamento & purificação , Inibidores de Glicosídeo Hidrolases/isolamento & purificação , Testes de Sensibilidade Microbiana , Estrutura Molecular , Policetídeos/isolamento & purificação
11.
Pak J Pharm Sci ; 32(4): 1537-1544, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31608872

RESUMO

In the present study, the ultrasound-assisted extraction (UAE) process of polysaccharide from Fructus corni (FCP) was opitimized using the Box-Behnken design (BBD). The main parameters including ultrasound time (min), temperature (oC) and solvent to raw material ratio (mL/g) were chosen as the process variables for the optimization of UAE process. The results of analysis of variance indicated that the equation obtained from the experiments could represent the data and the predicted responses satisfactorily. The optimum conditons obtained by BBD were ultrasonic time (51min), temperature (69oC) and solvent to solid ratio (20mL/g) with actual yield (12.68±0.16%), which was good agreement with value predicted by the model. The antioxidant properties of FCP were assessed in vitro based on scavenging effect of the DPPH radical, hydroxyl radical and super oxide radical tests. The FCP possessed strong antioxidant abilities on DPPH and super oxide radical at the high concentration. The results on hydroxyl radical demonstrated that FCP exhibited high scavenging effect when the concentration was over 3000µg/mL. The findings suggested that the FCP had antioxidant capacities and could be developed as a source of natural antioxidants and functional food material.


Assuntos
Antioxidantes/farmacologia , Fracionamento Químico/métodos , Cornus/química , Polissacarídeos/isolamento & purificação , Polissacarídeos/farmacologia , Análise de Variância , Antioxidantes/isolamento & purificação , Depuradores de Radicais Livres/isolamento & purificação , Depuradores de Radicais Livres/farmacologia , Frutas/química , Radical Hidroxila/química , Modelos Químicos , Extratos Vegetais/química , Polissacarídeos/análise , Solventes/química , Superóxidos/química , Temperatura , Ultrassom
12.
Appl Microbiol Biotechnol ; 103(19): 7943-7952, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31489456

RESUMO

In the present work, a novel cordycepic pigment was successfully isolated and identified from Cordyceps militaris, as well as named as cordycepene (C14H17N1O4), according to the long unsaturated conjugated polyene structural characteristic. Cordycepene is sensitive to light, high temperature (≥ 60 °C), and acidic condition (pH ≤ 3), but possesses high stability against metal ions, and under alkaline and neutral conditions. Cordycepene shows a comparable DPPH (1,1-diphenyl-2-picrylhydrazyl) radical-scavenging activity at higher concentration (≥ 2 mg/mL) to vitamin C. Cordycepene promotes the growth of HSF (human skin fibroblast cell) after incubation for 72 h, and has an ability to repair the UV light-treated HSF cells. In addition, cordycepene increases the antioxidant activity (SOD, superoxide dismutase; GSH-Px, glutathione peroxidase; CAT, catalase) and decreases MDA (malondialdehyde) level, indicating that cordycepene inhibits the photochemical senescence of HSF by enhancing the antioxidant defense system. The discovery of cordycepene can provide a basis for research on light incubation and the accumulation of yellow pigment (carotenoids) from C. militaris.


Assuntos
Cordyceps/química , Desoxiadenosinas/química , Desoxiadenosinas/isolamento & purificação , Pigmentos Biológicos/química , Pigmentos Biológicos/isolamento & purificação , Antioxidantes/química , Antioxidantes/isolamento & purificação , Sobrevivência Celular/efeitos dos fármacos , Células Cultivadas , Fibroblastos/efeitos dos fármacos , Fibroblastos/fisiologia , Depuradores de Radicais Livres/química , Depuradores de Radicais Livres/isolamento & purificação , Humanos , Concentração de Íons de Hidrogênio , Metais , Temperatura
13.
J Oleo Sci ; 68(9): 881-891, 2019 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-31413240

RESUMO

Enzyme-assisted aqueous extraction of rice germ oil (RGO) was performed in this study. The physicochemical properties, fatty acid composition, bioactive substances and antioxidant activity of RGO were analyzed. An enzyme composed of alcalase and cellulase (1:1, w/w) was found to be the most effective in the extraction yield of oil. The optimal oil yield of 22.27% was achieved under the conditions of an enzyme concentration of 2% (w/w), incubation time of 5 h, incubation temperature of 50°C, water to seed ratio of 5:1, and pH 6.0. The predominant fatty acids of RGO were oleic acid (39.60%), linoleic acid (34.20%) and palmitic acid (20.10%). The total saturated fatty acid (SFA), monounsaturated fatty acid (MUFA) and polyunsaturated fatty acid (PUFA) composition of RGO were 22.50%, 39.60% and 36.00%, respectively. RGO yielded a high content of γ-oryzanol (530 mg/100 g oil), tocotrienol (62.96 mg/100 g oil), tocopherol (23.24 mg/100 g oil) and a significant amount of phytosterol (372.14 mg/100 g oil). It exhibited notable antioxidant activities with IC50 values of 32.37 and 41.13 mg/mL, according to the DPPH radical scavenging assay and ß-carotene/linoleic acid bleaching test, respectively.


Assuntos
Depuradores de Radicais Livres/química , Oryza/química , Óleos Vegetais/química , Sementes/química , Celulase/química , Ácidos Graxos/análise , Depuradores de Radicais Livres/análise , Depuradores de Radicais Livres/isolamento & purificação , Fitosteróis/análise , Óleos Vegetais/análise , Óleos Vegetais/isolamento & purificação , Extração em Fase Sólida/métodos , Subtilisinas/química , Tocoferóis/análise
14.
Niger J Physiol Sci ; 34(1): 33-42, 2019 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-31449269

RESUMO

Diabetes Mellitus (DM) is a leading pan-systemic endocrine disorder with attendant high morbidity and mortality owing to its deleterious effects on vital body organs caused by untreated chronic hyperglycemia, attendant oxidative stress and glycation processes. The present study is designed to investigate possible protective role and mechanism(s) of action 125-500 mg/kg/day of Morinda lucida aqueous stem bark extract (MLASE) on renal and hepatic functions in alloxan-induced hyperglycemic rats for 8 days. Forty-two alloxan-induced hyperglycemiic male Wistar rats were randomly allotted to Groups II-VI and orally treated with 10 ml/kg/day distilled water, 5 mg/kg/day glibenclamide, 125 mg/kg MLASE, 250 mg/kg MLASE, and 500 mg/kg/day MLASE, respectively. Group I normal rats served as untreated control and were orally treated with 10 ml/kg of distilled water, all under same sham-handling. Blood samples were taken for measurement of fasting blood glucose, renal and hepatic function profile. Liver and kidney tissue samples were taken for determination of the activities of oxidative stress markers such as malondialdehyde (MDA), reduced glutathione (GSH), and glutathione peroxidase (GPx), catalase (CAT) and superoxidase dismutase (SOD). Results showed that intraperitoneal injection with 120 mg/kg of alloxan in cold 0.9% normal saline reliably and significantly induced a steadily sustained hyperglycemia which were ameliorated by short-term oral treatment with 125-500 mg/kg/day of MLASE, dose dependently, similar to that ameliorated by the standard antihyperglycemic drug, glibenclamide. Similarly, MLASE significantly mitigated against derangements in the measured renal and hepatic function parameters as well as oxidative stress induced by alloxan-induced hyperglycemia. In conclusion, results of this study showed the protective role of 125-500 mg/kg/day of MLASE in chronic hyperglycemia-associated renal and hepatic dysfunctions which was mediated via antioxidant and free radical scavenging activities of MLASE.


Assuntos
Diabetes Mellitus Experimental/tratamento farmacológico , Depuradores de Radicais Livres/uso terapêutico , Nefropatias/tratamento farmacológico , Hepatopatias/tratamento farmacológico , Morinda , Extratos Vegetais/uso terapêutico , Animais , Diabetes Mellitus Experimental/metabolismo , Relação Dose-Resposta a Droga , Depuradores de Radicais Livres/isolamento & purificação , Nefropatias/metabolismo , Hepatopatias/metabolismo , Masculino , Casca de Planta , Extratos Vegetais/isolamento & purificação , Caules de Planta , Ratos , Ratos Wistar , Água
15.
Chem Biodivers ; 16(10): e1900381, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31403756

RESUMO

The variability in the phytochemical concentrations of Capsicum annuum var. glabriusculum has not been extensively analyzed among wild populations and ecologic niches in its phylogeographic area. This study aimed to determine the variations in the phytochemical and antioxidant contents of the wild Piquin chili. The total flavonoid content of hydroalcoholic extracts (0.06 to 0.70 mg equivalent of quercetin per gram of dry weight (mg QE/g DW)), free radical-scavenging capacity for 2,2-diphenyl-1-picrylhydrazyl (DPPH. ) radicals (0.55 to 8.55 mm TE/g DW), amount of 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS+ ) in aqueous extracts (18.13 to 107.6 mm TE/g DW) and pungency (21,760 to 88,476 Scoville heat units) were highly variable. By analyzing the spatial distribution using the first three principal components, correlations between the phytochemical content and the free radical-scavenging capacity (in both extracts) and flavonoid and phenolic contents (in the hydroalcoholic extract) were observed. Consistent with the statistical analysis, the spatial analysis showed intraregional differences in composition patterns, with an emphasis on central and coastal areas. Flavonoid contents, polyphenol contents and free radical-scavenging activity were the phytochemical components that mainly contributed to the diversity of the population.


Assuntos
Compostos de Bifenilo/antagonistas & inibidores , Capsicum/química , Depuradores de Radicais Livres/farmacologia , Compostos Fitoquímicos/farmacologia , Picratos/antagonistas & inibidores , Ácidos Sulfônicos/antagonistas & inibidores , Tiazóis/antagonistas & inibidores , Depuradores de Radicais Livres/química , Depuradores de Radicais Livres/isolamento & purificação , Compostos Fitoquímicos/química , Compostos Fitoquímicos/isolamento & purificação
16.
Int J Biol Macromol ; 139: 801-809, 2019 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-31400421

RESUMO

In this study, two different processes of hot water (HW) and ultrasonic-assisted (UA) for the extraction of polysaccharide from ginger pomace (GPPs) were employed under their respective best parameters, and the characterization and antioxidant activity of the purified polysaccharide (HW-GPP1, HW-GPP2, HW-GPP3, and UA-GPP1, UA-GPP2, UA-GPP3, respectively) were analyzed. The data implied that the yield of the polysaccharide obtained by UA was higher than that of HW. Meanwhile, two kinds of GPPs possessed the different preliminary structural characteristics including molecular weight distributions, total sugar and protein content, uronic acid content, while similar monosaccharide compositions and sulfuric radical contents. In vitro antioxidant activity assays indicated that UA-GPP3 showed the strongest scavenging abilities on DPPH radicals, while UA-GPP2 possessed the strongest scavenging abilities on hydroxyl and superoxide radicals. Moreover, the antioxidant activity of each fractions of GPPs extracted by UA was better than that of the corresponding fractions of GPPs extracted by HW. These results showed that UA was more beneficial to enhance the extraction yields of the polysaccharides, and also resulted in GPPs with higher bioactivity. Therefore, it indicated that UA-GPPs could be used as a potential natural antioxidant. Accordingly, the ginger pomace could be used as a potential source for natural antioxidant.


Assuntos
Fracionamento Químico/métodos , Depuradores de Radicais Livres/química , Depuradores de Radicais Livres/isolamento & purificação , Gengibre/química , Polissacarídeos/química , Polissacarídeos/isolamento & purificação , Compostos de Bifenilo/química , Peso Molecular , Monossacarídeos/análise , Picratos/química
17.
Food Chem Toxicol ; 133: 110754, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31401185

RESUMO

The active components of Malus pumila flowers on antioxidant and tyrosinase activity were investigated with the method of spectrum-effect relationship and knock-out. Some compounds were identified by UPLC-MS/MS method. The chemical fingerprints were established by HPLC and the activity of antioxidant and tyrosinase were assayed in vitro. Chromatographic peaks P34, P35, P39, P44, P45 and P49 were identified as phloridzin, hyperoside, astragalin, afzelin, quercetin and kaempferol by UPLC-MS/MS method. Hyperoside and kaempferol were identified in M. pumila flowers for the first time. When the concentration was 1 g/mL of sample (equivalent to raw material), the scavenging capacity of P35 (hyperoside) on DPPH free radicals were consistent with the spectrum-effect relationship. The scavenging capacity of P34 (phloridzin) and P45 (quercetin) on ABTS free radicals were consistent with the spectrum-effect relationship. The activation effect of P45 (quercetin) on tyrosinase was consistent with the spectrum-effect relationship. The inhibitory effect of P34 (phloridzin), P35 (hyperoside) on tyrosinase were consistent with the spectrum-effect relationship.


Assuntos
Ativadores de Enzimas/química , Inibidores Enzimáticos/química , Flavonoides/química , Depuradores de Radicais Livres/química , Malus/química , Monofenol Mono-Oxigenase/antagonistas & inibidores , Cromatografia Líquida de Alta Pressão/métodos , Ativadores de Enzimas/isolamento & purificação , Inibidores Enzimáticos/isolamento & purificação , Flavonoides/isolamento & purificação , Flores/química , Depuradores de Radicais Livres/isolamento & purificação , Espectrometria de Massas em Tandem/métodos
18.
Mar Drugs ; 17(9)2019 Aug 28.
Artigo em Inglês | MEDLINE | ID: mdl-31466337

RESUMO

Some antioxidant compounds decrease the amount of intracellular reactive oxygen species (ROS) and consequently reduce the deleterious effects of ROS in osteoblasts. Thus, these compounds fight against osteoporosis. Brown seaweeds are a rich source of antioxidant fucose-containing sulfated polysaccharides (fucans and fucoidans). We obtained six fucoidans (FRFs)-F0.3, F0.5, F0.7, F1.0, F1.5, and F2.1-from Dictyota mertensii by proteolytic digestion followed by sequential acetone precipitation. Except for F0.3, all FRFs showed antioxidant activity in different in vitro tests. In pre- osteoblast-like cells (MC3T3-L1) exposed to H2O2-oxidative stress, caspase-3 and caspase-9 were activated, resulting in apoptosis of the cells. We also observed a decrease in superoxide dismutase (SOD) and alkaline phosphatase (ALP) activity. The antioxidant FRFs protected the cells from the oxidative damage caused by H2O2, decreasing intracellular ROS and caspase activation, and increasing SOD activity. The most effective protection against damage was provided by F0.7, F1.5, and F2.1. At 0.5 mg/mL, these FRFs also suppressed the H2O2-mediated inhibition of ALP activity. The data indicated that FRFs F0.7, F1.5, and F2.1 from D. mertensii were antioxidants that protected bone tissue from oxidative stress and could represent possible adjuvants for the treatment of bone fragility through counteracting oxidative phenomena.


Assuntos
Depuradores de Radicais Livres/farmacologia , Feófitas/química , Polissacarídeos/farmacologia , Alga Marinha/química , Células 3T3 , Animais , Apoptose/efeitos dos fármacos , Diferenciação Celular/efeitos dos fármacos , Depuradores de Radicais Livres/isolamento & purificação , Depuradores de Radicais Livres/uso terapêutico , Humanos , Peróxido de Hidrogênio/toxicidade , Camundongos , Osteoblastos/efeitos dos fármacos , Osteoblastos/fisiologia , Osteoporose/tratamento farmacológico , Oxirredução/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Polissacarídeos/isolamento & purificação , Polissacarídeos/uso terapêutico
19.
Zhongguo Zhong Yao Za Zhi ; 44(10): 2090-2095, 2019 May.
Artigo em Chinês | MEDLINE | ID: mdl-31355566

RESUMO

To isolate and identify secondary metabolites of marine-derived Streptomyces sp.MDW-06,the isolations and purifications of compounds were performed by means of column chromatography over silica gel. And their structures were elucidated through the spectroscopic analysis of MS,NMR and specific rotations. The bioactivities were assayed by paper diffusion and DPPH method. From the fermentation broth of marine-derived Streptomyces sp.MDW-06,five compounds( 1-5) were isolated and identified as streptopentanoic acid( 1),germicidin A( 2),germicidin B( 3),isogermicidin A( 4),isogermicidin B( 5) and oxohygrolidin( 6),respectively. Compound 1 is a new compound. Compound 1 shows DPPH radical scavenging activity with 36. 4% at 100 mg·L~(-1).


Assuntos
Depuradores de Radicais Livres/química , Policetídeos/química , Streptomyces/química , Cromatografia , Fermentação , Depuradores de Radicais Livres/isolamento & purificação , Espectroscopia de Ressonância Magnética , Policetídeos/isolamento & purificação
20.
Molecules ; 24(14)2019 Jul 23.
Artigo em Inglês | MEDLINE | ID: mdl-31340570

RESUMO

As a traditional natural medicine for treating many kinds of diseases, Gnetum parvifolium showed apparent inhibition on xanthine oxidase (XO). In this study, ultrafiltration combined with liquid chromatography-mass spectrometry (LC-MS) is used for the screening of XO inhibitors from Gnetum parvifolium. Their antioxidation, XO inhibition, and enzymic kinetic parameters are also determined. Finally, piceatannol (1), rhaponiticin (2), resveratrol (3), and isorhapontigenin (4) are screened out and identified as XO inhibitors from the extract of Gnetum parvifolium. Four inhibitors show better inhibition than allopurinol and good radical scavenging abilities. However, the antioxidant activities are weaker than ascorbic acid. The kinetic parameters illustrate the inhibition mode of XO by piceatannol is competitive type, while the inhibition modes for rhaponiticin, resveratrol and isorhapontigenin are uncompetitive types. In order to evaluate the difference among samples obtained in China, the amounts of four inhibitors and related activities in 20 samples are assessed and analyzed by partial least squares analysis. The results indicate piceatannol contribute the highest coefficients in three kinds of activities. Based on these findings, more comprehensive research on pharmaceutical and biochemical activities of these four XO inhibitors could be conducted in future.


Assuntos
Gnetum/química , Resveratrol/isolamento & purificação , Estilbenos/isolamento & purificação , Xantina Oxidase/antagonistas & inibidores , Alopurinol/farmacologia , Compostos de Bifenilo/antagonistas & inibidores , Inibidores Enzimáticos/isolamento & purificação , Inibidores Enzimáticos/farmacologia , Depuradores de Radicais Livres/isolamento & purificação , Depuradores de Radicais Livres/farmacologia , Ensaios de Triagem em Larga Escala , Cinética , Análise dos Mínimos Quadrados , Picratos/antagonistas & inibidores , Extratos Vegetais/química , Resveratrol/farmacologia , Estilbenos/farmacologia , Ultrafiltração , Xantina Oxidase/metabolismo
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