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1.
PLoS One ; 15(8): e0237844, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32834013

RESUMO

The feasibility of the production of germinated rice extracts using indigenous rice from Southern Thailand, including Khemtong (KHT) and Khai Mod Rin (KMR) from a single location at the Pak Phanang River Basin in Nakhon Si Thammarat, was investigated. The nutritional composition and bioactivity of the germinated rice extracts from both cultivars were evaluated. Optimum germination time for both rices was 96 h, leading to the highest GABA, thiamine, free amino acid, total sugar, and α-amylase activity (p<0.05). Germinated KHT had a higher α-amylase activity than germinated KMR at all germination times. Mashing at 60°C/pH 5.5 rendered the extract with the highest GABA content (p<0.05) and desirable contents of other nutrients. In comparison with germinated Sungyod (local colored rice) and Jasmine (commercial Thai rice) extracts, KHT and KMR showed a higher scavenging activity against DPPH•, OH•, and H2O2 (p<0.05) with a comparable ABTS•+ inhibition. For metal chelation, reducing power and ACE inhibitory activity, the germinated Sungyod extract was greater than KHT/KMR. The results demonstrated the potential use of germinated local Thai rice for the production of functional beverages.


Assuntos
Germinação , Fenômenos Fisiológicos da Nutrição , Oryza/química , Oryza/crescimento & desenvolvimento , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Aminoácidos/análise , Inibidores da Enzima Conversora de Angiotensina/farmacologia , Antioxidantes/farmacologia , Compostos de Bifenilo/química , Estudos de Viabilidade , Flavonoides/análise , Depuradores de Radicais Livres/química , Minerais/análise , Fenóis/análise , Ácido Fítico/análise , Picratos/química , Açúcares/análise , Tiamina/análise , Fatores de Tempo , alfa-Amilases/metabolismo , Ácido gama-Aminobutírico/análise
2.
Free Radic Res ; 54(7): 535-539, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32838569

RESUMO

The relationship between radical-scavenging rate constants (k) in an aprotic medium and thermodynamic parameters calculated by density functional theory (DFT) was investigated for 7 flavonols, which are myricetin (Myr), quercetin (Que), morin (Mor), kaempferol (Kae), 2'-methylquercetin (2'-MeQue), 5'-methylquercetin (5'-MeQue), and 2',5'-dimethylquercetin (Me2Que). The k values were determined for the reaction between the flavonols and galvinoxyl radical used as a reactivity model of reactive oxygen species in deaerated acetonitrile at 298 K. The energy difference values (D HT, HT: hydrogen transfer) between the flavonols and the corresponding radicals, which equal to the relative O-H bond dissociation energies of the OH groups in the flavonols and ionisation potentials (IP) were calculated by DFT at the B3LYP/6-31++G(d) level with C-PCM solvation model parameterised for acetonitrile. Among the 7 flavonols used in this study, calculated IP values of 4 flavonols exhibited a linear correlation with log k, suggesting that the radical-scavenging reaction of these flavonols may proceed via an electron transfer as the rate determining step.


Assuntos
Flavonóis/química , Depuradores de Radicais Livres/química , Antioxidantes/química , Compostos Benzidrílicos/química , Teoria da Densidade Funcional , Cinética , Espécies Reativas de Oxigênio/química , Relação Estrutura-Atividade , Termodinâmica
3.
Free Radic Res ; 54(7): 556-565, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32854555

RESUMO

This study aimed to develop a procedure for preparing a modified silica-naringin hybrid system. To accomplish, the properties of the obtained material were characterized by FT-IR analysis, UV-Vis spectrophotometry, thermogravimetry, scanning electron microscopy (SEM), and zeta potential. 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay was used to characterize and evaluate the antioxidant activity. The naringin release profile at pH = 1.2 and 7.2 were determined. FT-IR studies confirmed the interaction between the naringin and present carrier. The release study indicated that a release an approximately 20% and 50% of the release occurred in the first 30 min in pH = 1.2 and 7.2, respectively. The thermogravimetry and UV-Vis spectrophotometry analysis allowed us to determine the amount of naringin in the studied hybrid material at the level of several percent. The proposed hybrid material shows good stability, as evidenced by the zeta potential of about +30 and -30 mV in an acidic and alkaline environment, respectively. Antioxidant properties are comparable to those of pure naringin. The results suggest that the obtained hybrid material is a promising product with antioxidant properties.


Assuntos
Flavanonas/química , Depuradores de Radicais Livres/química , Dióxido de Silício/química , Antioxidantes/química , Flavanonas/síntese química , Depuradores de Radicais Livres/síntese química , Compostos de Silício/síntese química , Espectroscopia de Infravermelho com Transformada de Fourier , Termogravimetria
4.
J Nat Med ; 74(4): 796-803, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32632912

RESUMO

Two new tri-ferulates of sucrose, firmosides A and B (1 and 2, respectively), together with 18 known compounds (3-20), were isolated from the aerial parts of Silene firma. The structures of the isolated compounds were elucidated by various spectroscopic methods, including 1D, 2D NMR, and high-resolution electro-spray ionization-mass spectrometry (HR-ESI-MS). All the isolated compounds were evaluated for their free radical scavenging activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. As a result, two new compounds (1, 2) and 11 demonstrated significant radical scavenging activity, implying the usefulness as antioxidant agents.


Assuntos
Depuradores de Radicais Livres/química , Extratos Vegetais/química , Silene/química , Sacarose/química , Estrutura Molecular
5.
Int J Nanomedicine ; 15: 4021-4047, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32606662

RESUMO

Purpose: Periodontitis is a chronic inflammatory disease associated with microbial accumulation. The purpose of this study was to reuse the agricultural waste to produce cellulose nanofibers (CNF) and further modification of the CNF with κ-carrageenan oligosaccharides (CO) for drug delivery. In addition, this study is focused on the antimicrobial activity of surfactin-loaded CO-CNF towards periodontal pathogens. Materials and Methods: A chemo-mechanical method was used to extract the CNF and the modification was done by using CO. The studies were further proceeded by adding different quantities of surfactin [50 mg (50 SNPs), 100 mg (100 SNPs), 200 mg (200 SNPs)] into the carrier (CO-CNF). The obtained materials were characterized, and the antimicrobial activity of surfactin-loaded CO-CNF was evaluated. Results: The obtained average size of CNF and CO-CNF after ultrasonication was 263 nm and 330 nm, respectively. Microscopic studies suggested that the CNF has a short diameter with long length and CO became cross-linked to form as beads within the CNF network. The addition of CO improved the degradation temperature, crystallinity, and swelling property of CNF. The material has a controlled drug release, and the entrapment efficiency and loading capacity of the drug were 53.15 ± 2.36% and 36.72 ± 1.24%, respectively. It has antioxidant activity and inhibited the growth of periodontal pathogens such as Streptococcus mutans and Porphyromonas gingivalis by preventing the biofilm formation, reducing the metabolic activity, and promoting the oxidative stress. Conclusion: The study showed the successful extraction of CNF and modification with CO improved the physical parameters of the CNF. In addition, surfactin-loaded CO-CNF has potential antimicrobial activity against periodontal pathogens. The obtained biomaterial is economically valuable and has great potential for biomedical applications.


Assuntos
Carragenina/química , Celulose/química , Lipopeptídeos/química , Nanofibras/química , Peptídeos Cíclicos/química , Periodonto/microbiologia , Animais , Bactérias/metabolismo , Compostos de Bifenilo/química , Sobrevivência Celular , Difusão Dinâmica da Luz , Depuradores de Radicais Livres/química , Malondialdeído/metabolismo , Camundongos , Testes de Sensibilidade Microbiana , Nanofibras/ultraestrutura , Oligossacarídeos/química , Picratos/química , Células RAW 264.7 , Soja/química , Espectroscopia de Infravermelho com Transformada de Fourier
6.
Food Chem ; 332: 127408, 2020 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-32619944

RESUMO

A research was performed to determine and compare the physicochemical properties, chemical compositions and in vitro antioxidant activities of Paeonia lactiflora Pall seed oils with ultrasonic-assisted solvent extraction, pressing and supercritical fluid extraction. Paeonia lactiflora Pall seed oil contained a high percentage of polyunsaturated fatty acids and monounsaturated fatty acids, especially oleic (31.62-32.88%) and α-linolenic acids (37.55-39.95%). The beneficial multiple dietary phytochemicals (tocopherol, phytosterols and squalene) and in vitro antioxidant activity were significantly influenced by the hull and processing method (P<0.05). However, higher tocopherol (596.67-738.76 mg/kg) and phytosterols (5775.01-6055.62 mg/kg) contents were found in supercritical fluid extraction oils. Additionally, ten individual polyphenols were quantified, and significantly influenced by the hull and processing method (P<0.05), with the content of benzoic acid and several individual flavonoids being the higher. According to the results, pressing might be the best process for extracting oil with a high number of polyphenols.


Assuntos
Paeonia/metabolismo , Óleos Vegetais/química , Cromatografia com Fluido Supercrítico , Depuradores de Radicais Livres/química , Fitosteróis/química , Fitosteróis/isolamento & purificação , Extratos Vegetais/química , Óleos Vegetais/isolamento & purificação , Sementes/química , Sementes/metabolismo , Sonicação , Tocoferóis/química , Tocoferóis/isolamento & purificação
7.
Nat Commun ; 11(1): 2788, 2020 06 03.
Artigo em Inglês | MEDLINE | ID: mdl-32493916

RESUMO

Oxidative stress is associated with many acute and chronic inflammatory diseases, yet limited treatment is currently available clinically. The development of enzyme-mimicking nanomaterials (nanozymes) with good reactive oxygen species (ROS) scavenging ability and biocompatibility is a promising way for the treatment of ROS-related inflammation. Herein we report a simple and efficient one-step development of ultrasmall Cu5.4O nanoparticles (Cu5.4O USNPs) with multiple enzyme-mimicking and broad-spectrum ROS scavenging ability for the treatment of ROS-related diseases. Cu5.4O USNPs simultaneously possessing catalase-, superoxide dismutase-, and glutathione peroxidase-mimicking enzyme properties exhibit cytoprotective effects against ROS-mediated damage at extremely low dosage and significantly improve treatment outcomes in acute kidney injury, acute liver injury and wound healing. Meanwhile, the ultrasmall size of Cu5.4O USNPs enables rapid renal clearance of the nanomaterial, guaranteeing the biocompatibility. The protective effect and good biocompatibility of Cu5.4O USNPs will facilitate clinical treatment of ROS-related diseases and enable the development of next-generation nanozymes.


Assuntos
Cobre/química , Depuradores de Radicais Livres/química , Inflamação/patologia , Nanopartículas/química , Tamanho da Partícula , Espécies Reativas de Oxigênio/química , Lesão Renal Aguda/sangue , Lesão Renal Aguda/patologia , Lesão Renal Aguda/terapia , Alanina Transaminase/sangue , Animais , Antioxidantes/química , Aspartato Aminotransferases/sangue , Bovinos , Cobre/farmacocinética , Feminino , Células HEK293 , Humanos , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BL , Nanopartículas/ultraestrutura , Análise de Componente Principal , Ratos Sprague-Dawley , Distribuição Tecidual , Cicatrização
8.
PLoS One ; 15(6): e0234157, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32516332

RESUMO

Brazilian native fruits are a rich source of polyphenolic compounds that can act as anti-inflammatory and antioxidant agents. Here, we determined the polyphenolic composition, anti-inflammatory mechanism of action, antioxidant activity and systemic toxicity in Galleria mellonella larvae of Eugenia selloi B.D.Jacks. (synonym Eugenia neonitida Sobral) extract (Ese) and its polyphenol-rich fraction (F3) obtained through bioassay-guided fractionation. Phenolic compounds present in Ese and F3 were identified by LC-ESI-QTOF-MS. The anti-inflammatory activity of Ese and F3 was tested in vitro and in vivo through NF-κB activation, cytokine release and neutrophil migration assays. The samples were tested for their effects against reactive species (ROO•, O2•-, HOCl and NO•) and for their toxicity in Galleria mellonella larvae model. The presence of hydroxybenzoic acid, ellagitannins and flavonoids was identified. Ese and F3 reduced NF-κB activation, cytokine release and neutrophil migration, with F3 being three-fold more potent. Overall, F3 exhibited strong antioxidant effects against biologically relevant radicals, and neither Ese nor F3 were toxic to G. mellonella larvae. In conclusion, Ese and F3 revealed the presence of phenolic compounds that decreased the inflammatory parameters evaluated and inhibited reactive oxygen/nitrogen species. E. selloi is a novel source of bioactive compounds that may provide benefits for human health.


Assuntos
Eugenia/química , Frutas/química , Polifenóis/química , Polifenóis/farmacologia , Animais , Anti-Inflamatórios/química , Anti-Inflamatórios/farmacologia , Anti-Inflamatórios/toxicidade , Sobrevivência Celular/efeitos dos fármacos , Quimiocina CXCL2/metabolismo , Depuradores de Radicais Livres/química , Depuradores de Radicais Livres/farmacologia , Depuradores de Radicais Livres/toxicidade , Lepidópteros/efeitos dos fármacos , Masculino , Camundongos , NF-kappa B/metabolismo , Polifenóis/toxicidade , Células RAW 264.7 , Espécies Reativas de Nitrogênio/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Fator de Necrose Tumoral alfa/metabolismo
9.
Food Chem ; 330: 127325, 2020 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-32569939

RESUMO

In order to develop natural, food-grade particles as emulsifiers, wet-milled has been conducted to obtain apple pomace particles in varying sizes. Structural characteristics, physicochemical properties and Pickering emulsifying potential of the particle in different sizes were investigated. Particle size of apple pomace was gradually reduced from 12.9 µm to 550 nm during 8 h milling. With the decrease of particles size, the morphology became less angular. Meanwhile, some insoluble dietary fibers transformed into soluble ones, and the wettability tended to be hydrophilic, therefore, the water and oil holding capacities and free-radical-scavenging capacities increased. The properties of Pickering emulsions stabilized by wet-milled apple pomace particles in different sizes were then investigated. The decrease of particle size resulted in the size reduction of emulsion droplets, and gave rise to enhance gel-like properties and antioxidative activities of emulsions. The results demonstrated promising prospect of wet-milled apple pomace particles as emulsifiers in food industry.


Assuntos
Emulsificantes/química , Emulsões/química , Malus/química , Fibras na Dieta , Depuradores de Radicais Livres/química , Interações Hidrofóbicas e Hidrofílicas , Tamanho da Partícula , Água/química , Molhabilidade
10.
Food Chem ; 331: 127350, 2020 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-32590267

RESUMO

Fish by-products are excellent sources of collagen. Acid-soluble collagen (ASC) derived from a mixed by-product of different fish species was hydrolyzed to obtain peptide fractions and evaluate their biological and functional activities. All fractions obtained (F1: ≥30, F2: 10-30, F3: 5-10, F4: 1-5, and F5: ≤1kDa) exhibited antioxidant activity at concentrations of 5, 10, and 15 mg/mL. However, F5 registered the highest reducing power (absorbance 0.366) and hydroxyl-radical-scavenging activity (91%) at 15 mg/mL; whereas the highest DPPH scavenging activity (81%) was also detected in F5 at 5 mg/mL. The solubility of F1, F2, and F3 was ≥ 95% at pH 7. The highest foaming capacity (78%), foaming stability (60%), and emulsion stability index (42 min) were registered for F1. However, the highest emulsifying activity index (130 m2/g) was for F3. These results place collagen obtained from a mixed by-product of different fish species as a potential biotechnological alternative for the industry.


Assuntos
Antioxidantes/farmacologia , Colágeno/química , Produtos Pesqueiros , Proteínas de Peixes/química , Peptídeos/química , Peptídeos/farmacologia , Aminoácidos/análise , Animais , Antibacterianos/química , Antibacterianos/farmacologia , Antioxidantes/química , Emulsificantes/química , Proteínas de Peixes/farmacologia , Depuradores de Radicais Livres/química , Concentração de Íons de Hidrogênio , Hidrólise , Radical Hidroxila/química , Hidrolisados de Proteína/química , Solubilidade
11.
Int J Nanomedicine ; 15: 2515-2527, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32368038

RESUMO

Purpose: Reactive oxygen species (ROS)-induced oxidative stress plays a key role in the pathogenesis and progression of psoriasis by causing inflammation. Antioxidative strategies eradicating ROS may serve as effective and easy treatment options for psoriasis, while nanozymes with intrinsic antioxidant enzyme-like activity have not been explored for psoriasis treatment. The aim of this study is to fabricate ß-cyclodextrins (ß-CDs)-modified ceria nanoparticles (ß-CDs/CeO2 NPs) with drug-loaded and multimimic-enzyme activities for combinational psoriasis therapy. Methods: The ß-CDs/CeO2 NPs were synthesized by a hydrothermal method using unmodified ß-CDs as a protecting agent. The structure, size and morphology were analyzed by dynamic light scattering, transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared (FTIR) spectroscopy. Considering the superoxide dismutase (SOD)- and catalase-mimetic activities, the in vitro antioxidant activity of the ß-CDs/CeO2 NPs was investigated. After dithranol (DIT) was loaded, the drug-loading capacity and release profile were determined by UV-visible light spectrophotometer and high-performance liquid chromatography. The anti-psoriatic efficacy was studied in the imiquimod (IMQ)-induced mouse model on the basis of morphological evaluation, psoriasis area and severity index calculation (PASI), and inflammatory cytokine expression. Results: The average particle size of the blank ß-CDs/CeO2 NPs was 60.89±0.32 nm with a polydispersity index (PDI) of 0.12, whereas that of the DIT-loaded NPs was 79.38±1.06 nm with a PDI of 0.27. TEM results showed the as-prepared NPs formed a uniform quasi-spherical shape with low polydispersity. XPS indicates synthesized NPs have a mixed Ce3+/Ce4+ valence state. FTIR spectroscopy confirmed the presence of ß-CDs and DIT in the NPs. Inhibition of superoxide anion rate by NPs could be reached to 79.4% in the presence of 200 µg/mL, and elimination of H2O2 efficiency reached about 50% in the presence of 40 µg/mL, demonstrating excellent superoxide dismutase- and catalase-mimicking activities, thereby providing remarkable cryoprotection against ROS-mediated damage. Furthermore, ß-CDs on the surface endowed the NPs with drug-loading function via host-guest interactions. The entrapment efficiency and drug loading of DIT are 94.7% and 3.48%, respectively. The in vitro drug release curves revealed a suitable release capability of DIT@ß-CDs/CeO2 NPs under physiological conditions. In IMQ-induced psoriatic model, the DIT@ß-CDs/CeO2 NPs exhibited excellent therapeutic effect. Conclusion: This study may pave the way for the application of nanozyme ß-CDs/CeO2 NPs as a powerful tool for psoriasis therapy.


Assuntos
Cério/química , Nanopartículas/química , Psoríase/terapia , beta-Ciclodextrinas/química , Animais , Catalase/metabolismo , Linhagem Celular , Sobrevivência Celular , Terapia Combinada , Depuradores de Radicais Livres/química , Hidrodinâmica , Imiquimode/farmacologia , Imiquimode/uso terapêutico , Masculino , Camundongos Endogâmicos BALB C , Nanopartículas/ultraestrutura , Tamanho da Partícula , Espectroscopia Fotoeletrônica , Psoríase/tratamento farmacológico , Espécies Reativas de Oxigênio/metabolismo , Pele/efeitos dos fármacos , Pele/patologia , Espectroscopia de Infravermelho com Transformada de Fourier , Superóxido Dismutase/metabolismo , Fator de Necrose Tumoral alfa/metabolismo , beta-Ciclodextrinas/síntese química
12.
Chem Biol Interact ; 326: 109137, 2020 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-32442417

RESUMO

In the present study eighteen inhibitors of the hydrolytic enzymes of the endocannabinoid system were investigated for antioxidant activity using lipid peroxidation (LP) method. Among the assayed compounds ten belong to carbamates with phenyl [1,1'-biphenyl]-3-ylcarbamate (6), reported for the first time, and eight are retro-amide derivatives of palmitamine. Interestingly, results indicated that most of the tested compounds have good antioxidant properties. In particular, 1,3-di([1,1'-biphenyl]-3-yl)urea (3) shows IC50 = 26 ± 6 µM comparable to ones obtained for standard antioxidants trolox and quercetin (IC50 = 22 ± 6 µM and 23 ± 6 µM, respectively). Compound 3 was investigated further by means of DFT calculations, to clarify a possible mechanism of the antioxidant action. In order to estimate the capability of 3 to act as radical scavenger the structure was optimized at B3LYP/6-311++G** level and the respective bond dissociation enthalpies were calculated. The calculations in non-polar medium predicted as favorable mechanism a donation of a hydrogen atom to the free radical and formation of N-centered radical, while in polar solvents the mechanism of free radical scavenging by SPLET dominates over HAT H-abstraction. The possible radical scavenging mechanisms of another compound with potent antioxidant properties (IC50 = 53 ± 12 µM), the retro-amide derivative of palmitamine (compound 18), was estimated computationally based on the reaction enthalpies of a model compound (structural analogue to 18). The computations indicated that the most favorable mechanisms are hydrogen atom transfer from the hydroxyl group in meta-position of the benzamide fragment in nonpolar medium, and proton transfer from the hydroxyl group in ortho-position of the benzamide fragment in polar medium.


Assuntos
Compostos de Bifenilo/química , Peroxidação de Lipídeos/efeitos dos fármacos , Ureia/química , Anilidas/química , Antioxidantes/química , Benzamidas/química , Ácidos Graxos/química , Depuradores de Radicais Livres/química , Radicais Livres/química , Hidrogênio/química , Ácidos Palmíticos/química , Solventes/química
13.
PLoS One ; 15(4): e0232224, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32343717

RESUMO

The Amazon region is rich in genetic resources such as oilseeds which have potentially important local commercial exploitation. Despite its high concentration of bioactive compounds, cacay (Caryodendron orinocense Karst.) oil is poorly investigated and explored. Thus, this study focuses on the physicochemical characterization (moisture, density, and saponification, iodine, and acidity values), fatty acid composition as determined by gas chromatograph mass spectrometry (GC/MS), total phenolic content (TPC), and antioxidant activity (DPPH and ABTS radical scavenging assay) of cacay oil, coconut oil and a coconut/cacay oil blend, also known as cacay butter. The antibacterial activity of cacay oil was additionally evaluated. Our study demonstrated that cacay oil presents a high amount of polyunsaturated fatty acid (PUFA) (58.3%) with an emphasis on linoleic acid and a lower acidity value (2.67 ± 0.01 cg I2/g) than butter and coconut oil, indicating a low concentration of free fatty acids. In contrast, cacay butter and coconut oil presented higher saturated fatty acid percentages (69.1% and 78.4%, respectively) and higher saponification values (242.78 and 252.22 mg KOH/g, respectively). The samples showed low moisture and relative density between 912 and 916 kg/m3. The hydrophilic fraction of cacay oil was highlighted in the quantification of TPC (326.27 ± 6.79 mg GAE/kg) and antioxidant capacity in vitro by DPPH radical scavenging assay (156.57 ± 2.25 µmol TE/g). Cacay oil inhibited the growth of Bacillus cereus (44.99 ± 7.68%), Enterococcus faecalis (27.76 ± 0.00%), and Staphylococcus aureus (11.81 ± 3.75%). At long last, this is the first study reporting the physicochemical characterization and bioactive properties of cacay butter. Coconut oil and cacay butter showed great oxidative stability potential due to higher contents of saturated fatty acids. Moreover, cacay oil presents as an alternative source of raw materials for cosmetic and biotechnology industries due to its high concentration of PUFA and for being a rich source of phenolic compounds.


Assuntos
Óleo de Coco/química , Euphorbiaceae/química , Óleos Vegetais/química , Antibacterianos/química , Antibacterianos/farmacologia , Antioxidantes/química , Antioxidantes/farmacologia , Fenômenos Químicos , Óleo de Coco/farmacologia , Ácidos Graxos/química , Ácidos Graxos/farmacologia , Ácidos Graxos Insaturados/química , Ácidos Graxos Insaturados/farmacologia , Depuradores de Radicais Livres/química , Depuradores de Radicais Livres/farmacologia , Testes de Sensibilidade Microbiana , Fenóis/química , Fenóis/farmacologia , Compostos Fitoquímicos/química , Compostos Fitoquímicos/farmacologia , Óleos Vegetais/farmacologia
14.
Proc Natl Acad Sci U S A ; 117(12): 6910-6917, 2020 03 24.
Artigo em Inglês | MEDLINE | ID: mdl-32152121

RESUMO

Auxin is a class of plant hormone that plays a crucial role in the life cycle of plants, particularly in the growth response of plants to ever-changing environments. Since the auxin responses are concentration-dependent and higher auxin concentrations might often be inhibitory, the optimal endogenous auxin level must be closely controlled. However, the underlying mechanism governing auxin homeostasis remains largely unknown. In this study, a UDP-glycosyltransferase (UGT76F1) was identified from Arabidopsis thaliana, which participates in the regulation of auxin homeostasis by glucosylation of indole-3-pyruvic acid (IPyA), a major precursor of the auxin indole-3-acetic acid (IAA) biosynthesis, in the formation of IPyA glucose conjugates (IPyA-Glc). In addition, UGT76F1 was found to mediate hypocotyl growth by modulating active auxin levels in a light- and temperature-dependent manner. Moreover, the transcription of UGT76F1 was demonstrated to be directly and negatively regulated by PIF4, which is a key integrator of both light and temperature signaling pathways. This study sheds a light on the trade-off between IAA biosynthesis and IPyA-Glc formation in controlling auxin levels and reveals a regulatory mechanism for plant growth adaptation to environmental changes through glucosylation of IPyA.


Assuntos
Proteínas de Arabidopsis/metabolismo , Arabidopsis/crescimento & desenvolvimento , Regulação da Expressão Gênica de Plantas , Glucose/metabolismo , Hipocótilo/crescimento & desenvolvimento , Ácidos Indolacéticos/farmacologia , Indóis/metabolismo , Arabidopsis/efeitos dos fármacos , Arabidopsis/metabolismo , Arabidopsis/efeitos da radiação , Proteínas de Arabidopsis/genética , Depuradores de Radicais Livres/química , Depuradores de Radicais Livres/metabolismo , Glucosiltransferases/metabolismo , Glicosilação , Hipocótilo/efeitos dos fármacos , Hipocótilo/metabolismo , Hipocótilo/efeitos da radiação , Indóis/química , Luz , Reguladores de Crescimento de Planta/farmacologia , Plântula , Temperatura
15.
J Chromatogr A ; 1620: 460988, 2020 Jun 07.
Artigo em Inglês | MEDLINE | ID: mdl-32115233

RESUMO

Traditional Chinese medicine (TCM), especially herbal medicine compound preparation, faces great challenges in its quality control due to a myriad of components involved. How to perform quality control of TCM more effectively has been a research topic. In this study, we used Tianmeng oral liquid (TOL) as a case study and developed a comprehensive strategy based on non-targeted, targeted and bioactive analyses for quality evaluation of TOL from different batches. Firstly, a non-targeted fingerprinting analysis was performed by HPLC-DAD and UHPLC-MS/MS. Twenty-five batches of TOL were clearly discriminated by similarity analysis and hierarchical cluster analysis and components were tentatively identified. Secondly, the targeted quantitative methods based on HPLC-DAD and HPLC-MS were applied to simultaneous quantitative determination of five and eight marker compounds, especially toxic component strychnine, respectively. The quantitative data were processed with principal component analysis for differentiating different batches of samples. Finally, we explored the feasibility of establishing a total antioxidant capacity (TAC) model. How to use the peak area instead of the corresponding concentration to determine the antioxidant activity-related compounds was theoretically explained for the first time, which was of great significance for the study of the fingerprint-efficacy relationship. The orthogonal signal correction-partial least squares model was employed to predict the TAC of TOL from their chromatographic fingerprints and identify three potential antioxidant markers. These results demonstrated that the comprehensive strategy from fingerprinting, chemical composition, multiple-component quantification, and antioxidant activity could be applied to quality evaluation of TOL and discrimination of the expired and unexpired samples.


Assuntos
Medicamentos de Ervas Chinesas/química , Medicina Tradicional Chinesa , Administração Oral , Compostos de Bifenilo/química , Calibragem , Cromatografia Líquida de Alta Pressão/métodos , Depuradores de Radicais Livres/química , Análise dos Mínimos Quadrados , Limite de Detecção , Modelos Teóricos , Picratos/química , Análise de Componente Principal , Padrões de Referência , Reprodutibilidade dos Testes , Soluções
16.
J Fluoresc ; 30(2): 407-418, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32088852

RESUMO

The combination of an Ayurvedic wisdom and nanotechnology may help us to resolve the complex healthcare challenges. A facile and economical one-pot hydrothermal synthesis method has been adopted for preparing a blue fluorescent carbon dots (CDs) with a quantum yield of 15.10% from an Ayurvedic medicinal plant Andrographis paniculata (AP). The Andrographis paniculata derived CDs (AAPCDs) were then characterized using different techniques. Through High Performance Thin Layer Chromatography (HPTLC) profiling of the AP extract and the CDs, it was found that some of the phytoconstituents are retained as such while others may have been converted into their derivatives during the process of formation of CDs. The CDs are designed to possess cellular imaging of human breast carcinoma cells (MCF-7), apart from free radicals sensing and scavenging capabilities. AAPCDs showed minimal cytotoxicity in Multi Drug Resistant clinically isolated strains of gram positive and gram negative bacteria which may be employed for microbiology oriented experiments. These results suggest potential of multi-functional AAPCDs as nano-probes for various pharmaceutical, biomedical and bioengineering applications.


Assuntos
Andrographis/química , Antibacterianos/química , Corantes Fluorescentes/química , Depuradores de Radicais Livres/química , Imagem Óptica , Antibacterianos/farmacologia , Compostos de Bifenilo/antagonistas & inibidores , Carbono/química , Sobrevivência Celular/efeitos dos fármacos , Farmacorresistência Bacteriana Múltipla/efeitos dos fármacos , Corantes Fluorescentes/farmacologia , Depuradores de Radicais Livres/farmacologia , Humanos , Klebsiella pneumoniae/efeitos dos fármacos , Células MCF-7 , Medicina Ayurvédica , Testes de Sensibilidade Microbiana , Nanotecnologia , Tamanho da Partícula , Picratos/antagonistas & inibidores , Folhas de Planta/química , Pontos Quânticos/química , Espectrometria de Fluorescência , Staphylococcus aureus/efeitos dos fármacos , Propriedades de Superfície
17.
J Trauma Acute Care Surg ; 88(5): 677-685, 2020 05.
Artigo em Inglês | MEDLINE | ID: mdl-32039974

RESUMO

INTRODUCTION: Free radicals and reactive oxygen species are related to deteriorating pathological conditions after head trauma because of their secondary effects. 2,2,6,6-Tetramethylpiperidine-1-oxyl (TEMPO) scavenges free radicals; however, this molecule is also toxic. Here, we have evaluated the neuroprotective effect of antioxidant nanoparticles, which consisted of a novel core-shell type nanoparticle containing 4-amino-TEMPO, that is, redox-active nitroxide radical-containing nanoparticles (RNPs). METHODS: Institute of Cancer Research mice were subjected to a head-impact procedure, randomly divided into four groups and intravenously (3 mg/kg) administered phosphate-buffered saline, TEMPO, micelle (a self-assembling block copolymer micelle without a TEMPO moiety), or RNP through the tail vein immediately thereafter and intraperitoneally at days 1, 3, and 5 after traumatic brain injury (TBI). The RNP distribution was detected by rhodamine labeling. Cognitive behavior was assessed using the neurological severity score and a rotarod test at days 1, 3, and 7 following TBI, and contusion volume was measured at day 7 after TBI. Free radical-scavenging capacity was analyzed by electron paramagnetic resonance on day 1 after TBI, and immunostaining was used to observe mobilization of microglia (Iba-1) and rescued neuronal cells (NeuN). RESULTS: Redox-active nitroxide radical-containing nanoparticle was detected in the microvessels around the injured area in the brain. Cognitive behavior assessment was significantly better, and contusion volume was significantly smaller in the RNP group compared with the other groups. Superoxide anion scavenging capacity was significantly higher in the RNP group, and neuronal loss was significantly suppressed around the injured area at day 7 after TBI. Furthermore, in the RNP group, neurodegenerative microglia production was suppressed at days 3 and 7 after TBI, whereas neuroprotective microglia production was higher at day 7 after TBI. CONCLUSION: The RNP administration after TBI improved cognitive behavior and reduced contusion volume by improving reactive oxygen species scavenging capacity. Therefore, RNP may have a neuroprotective effect after TBI. LEVEL OF EVIDENCE: Therapeutic test.


Assuntos
Lesões Encefálicas Traumáticas/tratamento farmacológico , Depuradores de Radicais Livres/administração & dosagem , Fármacos Neuroprotetores/administração & dosagem , Espécies Reativas de Oxigênio/antagonistas & inibidores , Administração Intravenosa , Animais , Comportamento Animal/efeitos dos fármacos , Lesões Encefálicas Traumáticas/patologia , Cognição/efeitos dos fármacos , Óxidos N-Cíclicos/administração & dosagem , Óxidos N-Cíclicos/química , Modelos Animais de Doenças , Depuradores de Radicais Livres/química , Humanos , Masculino , Camundongos , Nanopartículas/administração & dosagem , Nanopartículas/química , Fármacos Neuroprotetores/química , Óxidos de Nitrogênio/administração & dosagem , Óxidos de Nitrogênio/química
18.
J Environ Sci Health B ; 55(5): 495-500, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32067557

RESUMO

The total polyphenolic content and the antioxidant activity have been analyzed in ground beans of green, light, medium and dark roasted coffee by UV-VIS spectrometry. Water coffee extracts showed the highest levels of polyphenols in green and light roasted coffees where the total polyphenolic content (TPC) ranged from 49.19 ± 0.70 to 74.05 ± 0.28 and from 59.79 ± 1.45 to 38.34 ± 1.26 g GAE.kg-1, respectively. In medium roast samples it ranged from 43.90 ± 3.07 to 74.05 ± 0.28g GAE.kg-1 and in dark roast from 37.44 ± 0.63 to 47.41 ± 0.69 g GAE.kg-1. The total antioxidant capacity (TAC) reached the highest values (DPPH inhibition ranging from 69.08 ± 1.33% to 78.55 ± 0.89%) in light roasted coffees. Dark roasted coffees showed both the lowest content of polyphenols as well as the total antioxidant capacity. In case of TPC, statistically significant differences (P˂0.001) have been identified between green coffee and other roasted degrees. Also, dark coffee showed statistically noticeable differences (P˂0.001) in TPC in relation to other roasted stages. Statistically important difference (P˂0.001) has been discovered between the total antioxidant capacity of dark roasted coffee and other roasting levels. The results demonstrated that roasting process affects both the oxidative activity as well as polyphenolic content.


Assuntos
Antioxidantes/química , Coffea/química , Indústria de Processamento de Alimentos/métodos , Polifenóis/química , Sementes/química , Café/química , Depuradores de Radicais Livres/análise , Depuradores de Radicais Livres/química , Extratos Vegetais/química , Polifenóis/análise , Espectrofotometria Ultravioleta , Água
19.
Pak J Biol Sci ; 23(2): 190-205, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31944079

RESUMO

BACKGROUND AND OBJECTIVE: Quarantine treatment enables export of mango fruits to parts of the world that enforce quarantine against fruit fly. Chilling injuries (CI) are prevalent in the mango fruits stored at temperatures lower than 12°C during cold-quarantine. So, this research examined the potentially enable of cold-shock treatment and integrated of edible coating with packing in (EPE) foam net to enhance the resistance of Naomi mango fruits during cold export. MATERIALS AND METHODS: The effect of cold quarantine was studied on fruit quality of 'Naomi' mango for 2016 and 2017 seasons. In this respect, mango fruits were coated with sodium alginate at 3%, semperfreshTM at 1% and packed in (EPE) foam net to reduce CI during cold quarantine. The treated fruits were stored for 4 h at 0°C, then transferred to 20°C for 20 h (cold-shock treatment) prior to store at 2±1°C and 90-95% RH for 15 days (quarantine treatment). While, the control fruit were directly stored at 2±1°C and 90-95% RH for 15 days. Thereafter, all fruits were stored at 20±2°C for and 70-75% RH for 7 days as shelf life period to simulate a marketing period. RESULTS: All applied treatments significantly reduced fruits weight loss (%), respiration rate, retard the loss of soluble solid (%) titratable acidity (%), have a good potential in maintaining firmness (lb inch-2), skin color h°, vitamin C, total phenol, flavonoids content, membrane stability index (MSI %), high rate of antioxidant capacity and retard the loss of shelf-life of fruits than the control. CONCLUSION: It was concluded that edible coatings used semperfreshTM at 1% with (EPE) foam net packing have a good potential in controlling postharvest chilling injury and maintaining the fruit quality being the most effective treatment on all parameters tested. It could potentially enable the export mango fruits to all quarantine-enforcing countries.


Assuntos
Conservação de Alimentos/métodos , Armazenamento de Alimentos/métodos , Mangifera , Antioxidantes/química , Ácido Ascórbico/química , Compostos de Bifenilo/química , Temperatura Baixa , Resposta ao Choque Frio , Flavonoides/química , Depuradores de Radicais Livres/química , Frutas/química , Gases , Metanol/química , Fenóis/química , Picratos/química , Extratos Vegetais/química , Superóxido Dismutase/metabolismo , Temperatura
20.
Molecules ; 25(2)2020 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-31963288

RESUMO

Non-enzymatic browning reactions between lipid aldehydes and aminophospholipids might play an important role in the oxidative stability of cold-pressed vegetable oils. We, therefore, aimed to study the Maillard-type reaction between hexanal, a lipid oxidation product of linoleic acid, and phosphatidylethanolamine (PE (16:0/18:1)) at a ratio of 2:1 at conditions representative of the extraction of cold-pressed soybean oils (CPSBO) and determine the radical scavenging activity of the carbonyl-amine adducts with the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The reaction product, 2-pentyl-3,5-dibutyl-dihydropyridine, could be identified by means of LC-ESI-QTOF-MS/MS. The formation of this nitrogen-containing heterocycle significantly increased with time and temperature (p < 0.05). The products formed during the carbonyl-amine reaction between PE (16:0/18:1) and hexanal at 60 °C showed a radical scavenging activity of approximately 20% (p < 0.05). The fraction, containing 2-pentyl-3,5-dibutyl-dihydropyridine, contributed to, but was not solely responsible for, the radical scavenging activity (p < 0.05). Incubation of CPSBO fortified with PE (16:0/18:1) at 60 °C for 60 min had the strongest radical scavenging activity of 85.1 ± 0.62%. Besides 2-pentyl-3,5-dibutyl-dihydropyridine, other carbonyl-amine adducts might impact the radical scavenging activity of CPSBO as well. The oxidative stability of CPSBO might be increased by promoting the formation of carbonyl-amine reaction products, such as 2-pentyl-3,5-dibutyl-dihydropyridine.


Assuntos
Aminas/química , Depuradores de Radicais Livres/química , Depuradores de Radicais Livres/farmacologia , Fosfatidiletanolaminas/química , Óleo de Soja/química , Aldeídos/química , Cromatografia Líquida , Metabolismo dos Lipídeos , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Oxirredução , Relação Estrutura-Atividade , Espectrometria de Massas em Tandem
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