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1.
J Physiol Anthropol ; 39(1): 1, 2020 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-31952553

RESUMO

BACKGROUND: Dermatoglyphics has been used widely in fields of medicine as a non-invasive diagnostic tool and an early assessment of risk for certain medical conditions. It reflects disturbances in fetal development during early prenatal weeks 14-22 when fingerprints develop. Dermatoglyphic asymmetry has been used to measure developmental instability during a specific period of human fetal development. Thus, the present study was planned to investigate whether digital and palmar dermatoglyphics of chronic kidney disease of unknown origin (CKDu) patients in Sri Lanka are different from healthy people. METHODS: A case control study was carried out among CKDu patients (90 males, 90 females) from a CKDu endemic area and gender-matched two control groups; one group from a CKDu endemic region (90 males, 90 females) and another group from a CKDu non-endemic region (90 males, 90 females). Dermatoglyphics were obtained using photographic methods. Both qualitative and quantitative dermatoglyphic variables were defined and analyzed according to standard criteria. Both directional (DA) and fluctuating asymmetry (FA) were assessed. RESULTS: Several qualitative dermatoglyphic variables had significant association with CKDu. The triradii a1 variable was less evident in palms of CKDu cases in both genders when compared to both control groups. The FA of pattern discordance (right vs left hands) between CKDu cases and control group were significant in several digits. The FA of the ridge count was found significant in several digits, and also significant for A-B ridge count and total ridge count. CONCLUSION: Based on these results, it is proposed that the mechanisms responsible for the development of CKDu might be associated with those responsible for FA observed in CKDu patients. Accordingly, a diagnostic tool based on FA could be developed for predicting risk prior to the development of CKDu.


Assuntos
Dermatoglifia , Insuficiência Renal Crônica/epidemiologia , Insuficiência Renal Crônica/fisiopatologia , Adulto , Idoso , Estudos de Casos e Controles , Feminino , Dedos/anatomia & histologia , Humanos , Masculino , Pessoa de Meia-Idade , Sri Lanka/epidemiologia
2.
J Forensic Sci ; 65(1): 84-89, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31454431

RESUMO

Impressions of friction ridges left on a surface are important evidence for identifying an individual and can be used to confirm his or her presence at the scene of an incident. Factors influencing the durability of fingerprints include the physiological characteristics and cleanliness of the individual, environmental factors, and time. The aim of the study was to determine the effect of atmospheric factors, gender, hand cleanliness and time on the width of friction ridge impressions and the amount of material forming the print. The research shows that factors such as gender, ambient conditions, and hand cleanliness affect the width of the ridge impressions and the quantity of material forming the print. The passage of time significantly reduces both the width of the ridge impressions and the quantity of the material forming the print.


Assuntos
Dermatoglifia , Higiene das Mãos , Manejo de Espécimes , Adulto , Escuridão , Exposição Ambiental , Feminino , Humanos , Luz , Masculino , Microscopia , Fatores Sexuais , Creme para a Pele , Temperatura Ambiente , Fatores de Tempo , Adulto Jovem
3.
Forensic Sci Int ; 306: 110049, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31785509

RESUMO

Crime-scenes are the heart of any investigation in terms of recovery of key forensic evidence - fingermarks and DNA. Yet, quite often, the operational work of the forensic units, in which different chemicals and reagents for fingermarks development are applied, can also be highly destructive to the property and the environment. Hence, for both ecological and intelligence purposes, an operational method that minimizes that damage would be ideal. In this study, an "out-of-the-box" approach is proposed; rather than working at the crime scene itself, gel-lifters were used as a transferring tool for the latent forensic evidence, fingermarks and DNA, to be developed by applying black wet-powder (Wetwop®) in a controlled laboratory environment, leaving the crime-scene undisturbed. The results show a promising potential, as this indirect method proved to be robust and successful for non-porous surfaces, and even for aged fingermarks. In addition, this study gave a new perspective into the accepted notion regarding the difference between male and female donors, showing a direct correlation of the size and weight of the hands to the natural pressure applied by the donor. Moreover, the indirect nature of the method highlights the importance of the interaction between the type of surface and the latent fingermark constituents, primarily water, and its effect on the survivability and quality of the developed fingermark. Due to the growing demand of keeping crime scenes and exhibits unharmed by both police and intelligence forces, the new approach of this application provides a valuable asset for future operational field-work.


Assuntos
Dermatoglifia , Manejo de Espécimes/instrumentação , Manejo de Espécimes/métodos , Adulto , DNA/isolamento & purificação , Impressões Digitais de DNA , Feminino , Humanos , Masculino , Repetições de Microssatélites , Pessoa de Meia-Idade , Projetos Piloto , Propriedades de Superfície , Tato
4.
Forensic Sci Int ; 307: 110119, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31869652

RESUMO

It is important that fingermark enhancement techniques are safe and simple to carry out. Many chemicals are widely used to enhance and develop bloody fingermark. However, the use of natural products for fingermark detection and examination has several advantages and challenges. In this study, Lac dye (Laccifer lacca) was used to enhance bloody fingermarks on various types of non-porous and porous materials. Bloody fingermarks were deposited using a depletion series technique on eleven different surfaces. To assess the efficiency of Lac dye stain, comparisons were performed with Amido black stain as a reference method. Results revealed the similarity between Lac dye and Amido black on non-porous materials, in terms of both fingermark grades, and color intensity. However, Lac dye showed relatively low performance for enhancing and developing bloody fingermarks on porous materials. This indicates that Lac dye can be beneficially used as an alternative to chemicals such as Amido black on a non-porous surface. Further study into Lac dye formulation on porous materials is recommended.


Assuntos
Compostos Azo , Manchas de Sangue , Dermatoglifia , Aumento da Imagem/métodos , Corantes , Humanos , Masculino , Naftalenossulfonatos , Porosidade
5.
Chemistry ; 26(12): 2741-2748, 2020 Feb 26.
Artigo em Inglês | MEDLINE | ID: mdl-31886910

RESUMO

To achieve a highly efficient, dual-state emission platform for picric acid (PA) detection and latent fingerprint (LFP) visualization, flexible alkyl chains have been facilely attached to the commercial organic dye 3,4,9,10-perylenetetracarboxylic dianhydride to provide the target perylenetetracarboxylate molecules PTCA-C4, PTCA-C6, and PTCA-C12. Interestingly, all these molecules exhibited impressive fluorescence characteristics with high photoluminescence quantum yields (PLQYs) of around 93.0 % in dilute solution. Also, emissive features were observed in the solid state because close molecular packing is prevented by the alkyl chains, especially for PTCA-C6, which has a high PLQY value of 49.0 %. Benefiting from its impressive fluorescence performance in both solution and as aggregates, PTCA-C6 was used as a dual-state emission platform for PA detection and also LFP visualization. For example, double-responsive fluorescence quenching in solution was observed in PA detection studies, resulting in high quenching constants (KSV ) and also low limit-of-detection values. Furthermore, the fingerprint powder based on PTCA-C6 also presented an impressive performance on various substrates in terms of fluorescence intensity and resolution, clearly providing the specific fine details of latent fingerprints. These results demonstrate that the facilely synthesized PTCA-C6 with efficient dual-state emission exhibits great potential in the real-world applications of PA detection and LFP visualization.


Assuntos
Anidridos/química , Dermatoglifia , Corantes Fluorescentes/síntese química , Perileno/análogos & derivados , Picratos/análise , Teoria da Densidade Funcional , Humanos , Limite de Detecção , Estrutura Molecular , Perileno/química , Espectrometria de Fluorescência
6.
Forensic Sci Int ; 307: 110076, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31862657

RESUMO

The recovery of fingermarks and DNA from the same location at a crime scene can be problematic because of contamination issues associated with powdering or laboratory-based visualisation processes and/or the perceived destructive impact of commonly employed 'swabbing' approaches to DNA recovery. Previous research in a controlled environment demonstrated that it was possible to recover DNA and latent fingermarks from the same location on various substrates when an adhesive approach to DNA recovery was used. The aim of this research was to conduct a pseudo-operational trial into the dual recovery of DNA and fingermarks using gel lifters for DNA recovery. Participants were asked to voluntarily and anonymously donate a wide variety of porous and non-porous substrates post handling. No instruction as to fingermark deposition nor environmental storage was provided. BVDA gel lifters were applied to the substrates to replicate DNA recovery followed by the application of fingermark visualisation processes. The number and quality of the fingermarks was established using a grading approach. Application factors were also investigated to consider the effects of user variation. The results demonstrated that it was possible to recover DNA and fingermarks considered to be capable of supporting an identification. Fingermark quality post lifting was dependant on the substrates used. The weight applied to the gel during its application was a lesser contributing factor than the duration of its contact with the surface. There was a greater chance of leaving the fingermarks unaltered with the application of a low weight and instantaneous retraction.


Assuntos
DNA/isolamento & purificação , Dermatoglifia , Ciências Forenses/métodos , Alumínio , Cianoacrilatos , Géis , Humanos , Indicadores e Reagentes , Ninidrina , Porosidade , Pós , Manejo de Espécimes/instrumentação , Propriedades de Superfície , Volatilização
7.
Forensic Sci Int ; 305: 110005, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31698202

RESUMO

Traces of condom lubricants in fingerprints can be valuable information in cases of sexual assault. Ideally, not only confirmation of the presence of the condom but also determination of the type of condom brand used can be retrieved. Previous studies have shown to be able to retrieve information about the condom brand and type from fingerprints containing lubricants using various analytical techniques. However, in practice fingerprints often appear latent and need to be detected first, which is often achieved by cyanoacrylate fuming. In this study, we developed a desorption electrospray ionization mass spectrometry (DESI-MS) method which, combined with principal component analysis and linear discriminant analysis (PCA-LDA), allows for high accuracy classification of condom brands and types from fingerprints containing condom lubricant traces. The developed method is compatible with cyanoacrylate (CA) fuming. We collected and analyzed a representative dataset for the Netherlands comprising 32 different condoms. Distinctive lubricant components such as polyethylene glycol (PEG), polydimethylsiloxane (PDMS), octoxynol-9 and nonoxynol-9 were readily detected using the DESI-MS method. Based on the analysis of lubricant spots, a 99.0% classification accuracy was achieved. When analyzing lubricant containing fingerprints, an overall accuracy of 90.9% was obtained. Full chemical images could be generated from fingerprints, showing the distribution of lubricant components such as PEG and PDMS throughout the fingerprint, while still allowing for classification. The developed method shows potential for the development of DESI-MS based analyses of CA treated exogenous compounds from fingerprints for use in forensic science.


Assuntos
Preservativos , Cianoacrilatos , Dermatoglifia , Lubrificantes , Espectrometria de Massas por Ionização por Electrospray , Análise Discriminante , Feminino , Ciências Forenses/métodos , Humanos , Masculino , Análise de Componente Principal
8.
Sud Med Ekspert ; 62(5): 33-38, 2019.
Artigo em Russo | MEDLINE | ID: mdl-31626192

RESUMO

The possibilities of determining the sex and age group of an adult on the basis of frequency analysis of combinations of dermatoglyphic signs are presented. The study established new, highly informative dermatoglyphic markers of general physiological traits, with a multiplicity of differences from 5 to 59 (p<0.05) in alternative comparison groups formed by sex and age groups of 18 to 35 years and 36 to 77 years. It was found that the combination of acquired signs of raised skin (white lines and scars) is more informative if compared to the combination of congenital signs (types of patterns, outlines of papillary ridges). Depending on the number of fingers studied, the total occurrence of informative combinations can be 37.8 - 64.4% (one finger) to 96.9% (5 fingers of one hand). The result opens up the prospect for the application of a dermatoglyphic research method in the identification of persons from fragmented human remains and fingerprints found at the scene of an accident.


Assuntos
Dermatoglifia , Dedos , Adolescente , Adulto , Idoso , Criança , Pré-Escolar , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Projetos de Pesquisa , Pele , Adulto Jovem
9.
Forensic Sci Int ; 302: 109904, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31472445

RESUMO

Fingermarks are highly relevant in criminal investigations for individualization purposes. In some cases, the question in court changes from 'Who is the source of the fingermarks?' to 'How did the fingermark end up on the surface?'. In this paper, we explore the evaluation of fingermarks given activity level propositions by using Bayesian networks. The variables that provide information on activity level questions for fingermarks are identified and their current state of knowledge with regards to fingermarks is discussed. We identified the variables transfer, persistency, recovery, background fingermarks, location of the fingermarks, direction of the fingermarks, the area of friction ridge skin that left the mark and pressure distortions as variables that may provide information on how a fingermark ended up on a surface. Using three case examples, we show how Bayesian networks can be used for the evaluation of fingermarks given activity level propositions.


Assuntos
Dermatoglifia , Teorema de Bayes , Medicina Legal/métodos , Humanos , Tato
10.
Forensic Sci Int ; 302: 109905, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31394460

RESUMO

Fingerprint detection and Short Tandem Repeat (STR) typing are two approaches used for identification of individuals. The main goal of forensic laboratories is the development of a standardized protocol to obtain an STR profile from latent fingerprints, by typing the DNA transferred onto touched objects. The results obtained in this field derive from studies conducted under controlled laboratory conditions. Here, for the first time, we report two different profiles obtained by DNA purified by latent fingerprints enhanced with dactyloscopic powders at a crime scene, 14 years previously. DNA extraction phase was optimized to improve removal of powder and automatically conducted. Despite the low concentration of purified DNA, it was not degraded. Even if quality of the profile is influenced by several factors such as the method of acquisition and storage conditions of the fingerprint, results obtained are adequately informative and could be uploaded to the CODIS database.


Assuntos
Impressões Digitais de DNA/métodos , DNA/isolamento & purificação , Dermatoglifia , Repetições de Microssatélites , Eletroforese Capilar , Humanos , Pós , Reação em Cadeia da Polimerase em Tempo Real , Análise de Regressão , Manejo de Espécimes/métodos
11.
Sud Med Ekspert ; 62(4): 22-29, 2019.
Artigo em Russo | MEDLINE | ID: mdl-31407702

RESUMO

Gender determination through the use of handprints that are devoid of dermatoglyphic features is associated with either a visual assessment of the shape of the palms and phalanges, or with a comparison of their size with the size of the hands of people of the opposite sex. AIM: To prove the possibility of gender determination from handprints without papillary ridge features by means of discriminant analysis of their dimensional characteristics, and to compare discriminant models with the results of visual sex classification and data of other authors. MATERIAL AND METHODS: The handprints of 162 adult subjects (68 males and 94 females), were examined using metrics that are usually not measured on the human body, such as the length of the hand without the distal phalanx of the third finger, the length of the hand from the center of the palm, the width of the ulnar part of the palm, the diagonal width of the palm, and the length of the first beam. The table editor Excel 2003 and statistical software package SPSS 14.0 for Windows have been used to carry out the statistical processing of the material. We used a multidimensional discriminant analysis by Fisher's method in a step-by-step version. RESULTS: All the proposed measurements of the handprint meet the reliability requirements (rTEM5%; R>0.950). The accuracy of discriminant functions varies from 80 to 90.6%, which is noticeably higher compared to the visual definition (62.0-68.0%). Verification of the models (6 cases out of 8) proved the possibility of significant improvement of human gender determination when using the calculation method, compared with the visual method. The high data content of the additional planimetric parameters suggests the suitability of the gender determination models at different degrees of fragmentation of handprints. A necessary condition for the measurements on the handprint is the absence of any visible distortion of its proportions. The results of the gender analysis when measuring a handprint are comparable with the data obtained from skeletal material, from the hand itself and from its dermatoglyphic prints. CONCLUSION: this study may prove useful in determining human gender through measurements of handprints that are devoid of dermatoglyphic features during a forensic investigation.


Assuntos
Mãos/anatomia & histologia , Sexo , Adulto , Dermatoglifia , Feminino , Dedos , Humanos , Masculino , Reprodutibilidade dos Testes
13.
J Forensic Sci ; 64(6): 1913-1915, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31283022

RESUMO

We present a case of a faux blood fingermark, wherein a natural latent fingerprint on the pistol at the crime scene was enhanced by the whole blood of the victim. A male was shot to death. The crime scene investigator did not find any blood fingermark during the collection of evidence, but a blood fingerprint was noticed in the firearms examination. We identified that the fingerprint was of the victim; a series of experiments on the nonporous substrate were then conducted; the death in this case was classified as a suicide. The phenomenon is interesting, and thus, calls for attention of the forensic community.


Assuntos
Sangue , Dermatoglifia , Armas de Fogo , Suicídio , Ciências Forenses/métodos , Humanos , Masculino
14.
Forensic Sci Med Pathol ; 15(3): 445-452, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31321632

RESUMO

Optical coherence tomography (OCT) is an interferometric imaging technique that has revolutionized clinical ophthalmology since the first half of the 1990's. Despite this approach being successfully employed in ophthalmology and having great potential in forensic cases, its use in different forensic fields appears to be quite limited. In this review we reviewed the scientific literature regarding the application of OCT in forensic science and legal medicine from 1995 to 2019. Our research showed the usefulness of this approach for the study of coronary injuries, postmortem ocular changes, forensic entomology, and several other applications of specific forensic interest (the study of blood stains, fingerprints, and hair bulbs for personal identification, as well as the study of materials found in the crime scene for comparation, or anti-fraud investigation). The creation of specific 'ad hoc' devices and a better knowledge of this type of technology by pathologists will be a fundamental step to continue to develop the use of OCT forensic fields.


Assuntos
Ciências Forenses , Tomografia de Coerência Óptica , Animais , Artrópodes , Manchas de Sangue , Vasos Coronários/diagnóstico por imagem , Dermatoglifia , Entomologia , Olho/diagnóstico por imagem , Cabelo/diagnóstico por imagem , Coração/diagnóstico por imagem , Humanos , Mudanças Depois da Morte
15.
J Forensic Leg Med ; 66: 155-161, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31306915

RESUMO

The simultaneous localisation and globalisation of 'terrorist threats' and cross-border criminality have led to increased expansion of surveillance activities and greater cross-border police and judicial cooperation, placing a greater priority on these activities within the political agenda of the EU. In this scenario, the expansion of technological systems for surveillance and monitoring, and the large-scale exchange of citizens' personal data play a pivotal role in the "fight against crime". This paper explores the multiplicity of data protection regimes in different EU Member States within the framework of the Prüm system. While EU regulations establish minimum standards for personal data flows at the transnational level, local and domestic practices are extremely heterogeneous. Based on analysis of 37 interviews conducted with professionals involved in the automated exchange of forensic genetic profiles, this paper provides empirical data that highlights the tensions between the local and the global within DNA data exchanges across the EU. These tensions relate to differentiated sociotechnical imaginaries regarding the protection of personal data flowing between Member-States. In sum, this paper analyses the potential threats to human rights created by the exchange of personal data with regards to issues of privacy and data protection.


Assuntos
Segurança Computacional/legislação & jurisprudência , Bases de Dados de Ácidos Nucleicos , Disseminação de Informação/legislação & jurisprudência , Cooperação Internacional , Privacidade/legislação & jurisprudência , Crime/prevenção & controle , Impressões Digitais de DNA/legislação & jurisprudência , Dermatoglifia , União Europeia , Humanos , Terrorismo/prevenção & controle
16.
J Am Podiatr Med Assoc ; 109(3): 187-192, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-31268787

RESUMO

BACKGROUND: Arch height is an important indicator of risk of foot pathology. The current non-invasive gold standard based on footprint information requires extensive pre-processing. Methods used to obtain arch height that are accurate and easier to use are required in routine clinical practice. METHODS: The proposed arch index diagonals (AId) method for determining the arch index (AI) reduces the complexity of the preprocessing steps. All footprints were first prepared as required by the Cavanagh and Rodgers method for determining the AI and then compared to the proposed diagonals method. Results were classified according to the Cavanagh and Rodgers cut-off values into three groups of low, normal and high AI. ANOVA and Tukey's post hoc tests were applied to identify significant differences between AI groups. Linear modeling was applied to determine the fit of the new AId method compared to the Cavanagh and Rodgers AI. RESULTS: One hundred and ninety-six footprints were analyzed. The ANOVA indicated significant differences between the groups for AId (F1,194=94.49, p<0.0001) and the Tukey post hoc tests indicated significant differences between the pair-wise comparisons (p<0.001). Linear modeling indicated that the AId ratio classified more footprints in the high arch group compared to Cavanagh and Rodgers results (R2=32%, p< 0.01). Intra- and inter-rater correspondence was above 90% and confirmed that the AId results provided a better indication of arch height. CONCLUSIONS: The proposed method simplifies current processing steps to derive the arch height.


Assuntos
Antropometria/métodos , Dermatoglifia , Pé/anatomia & histologia , Análise de Variância , Humanos , Modelos Lineares , Valores de Referência , Reprodutibilidade dos Testes
17.
Forensic Sci Int Genet ; 42: 21-30, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-31212206

RESUMO

Proteomic genotyping detects single amino acid polymorphisms to infer the genotype of corresponding non-synonymous SNPs. Like any DNA genotype, these inferences can be used to estimate random match probability. Fingermarks are a common source of biological evidence that is sample limited and a highly variable source of identifying DNA. Genetically variant peptides from fingermarks, that contain single amino acid polymorphisms, are an additional source of identifying genetic information. To discover these peptide biomarkers epidermal corneocytes from 9 subjects were isolated, processed, digested with trypsin and applied to mass spectrometry. The resulting proteomic and matching exome datasets were used to discover, characterize and validate 60 genetically variant peptides. An average of 28.8 ± 4.4 genetically variant peptides were detected from each subject resulting in a total of 264 SNP allele inferences with 260 true and 4 false positives, a false discovery rate of 1.5%. Random match probabilities were estimated using the genotype frequencies from the matching major populations in the 1000 Genomes Project. Estimates ranged up to a value of 1 in 1.7 × 108, with a median probability of 1 in 2.4 × 106. Furthermore, the proteomically-inferred genotypes are likely to be compatible with the STR-based random match probability estimates since the closest STR locus was 2.2 Mb from the nearest GVP-inferred SNP. This project represents a novel mode of genetic information that can be obtained from fingermarks and has the potential to complement other methods of human identification including analysis of ridge patterns or touch DNA.


Assuntos
Dermatoglifia , Células Epidérmicas/metabolismo , Genótipo , Peptídeos/genética , Proteoma/genética , Alelos , Cromatografia Líquida , Perfilação da Expressão Gênica/métodos , Humanos , Espectrometria de Massas , Peptídeos/metabolismo , Polimorfismo de Nucleotídeo Único , Proteoma/metabolismo , Proteômica
18.
Analyst ; 144(15): 4569-4574, 2019 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-31225569

RESUMO

A high quantum yield (QY) is the key requirement for implementing carbon dots (CDs) in nearly all applications. In this work, blue emissive N-doped CDs with a QY of 83% and orange emissive N-doped CDs with a QY of 47% were successfully prepared using resorcinol and phloroglucin as carbon resources in formamide by one-step microwave synthesis, respectively. Formamide not only plays a role as the solvent but also takes part in the formation of the high QY CDs. It is demonstrated that the as-prepared blue- and orange-emitting N-doped CDs with a high QY can be uniformly dispersed into glue and be fabricated as CD/glue fluorescent composites for fluorescent films and fingerprint imaging. Furthermore, these CDs also show excellent cellular imaging capability.


Assuntos
Carbono/química , Pontos Quânticos/química , Adesivos/química , Carbono/efeitos da radiação , Carbono/toxicidade , Cor , Dermatoglifia , Fluorescência , Humanos , Células MCF-7 , Membranas Artificiais , Microscopia de Fluorescência , Micro-Ondas , Floroglucinol/química , Pontos Quânticos/efeitos da radiação , Pontos Quânticos/toxicidade , Resorcinóis/química , Solubilidade
20.
Forensic Sci Int ; 300: 99-105, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31085432

RESUMO

A novel method for detection and visualization of latent DNA using Diamond™ Nucleic Acid Dye (DD) staining has been developed. Applying DD to an object has the real potential to visualize DNA on a substrate from which a DNA profile can be generated, but it is important to determine whether this staining will adversely affect other forensic investigational techniques and vice versa. The aim of this study was to examine the interactions between staining a fingermark to detect DNA and then generate a DNA profile in combination with several standard latent fingermark enhancement methods. Six common fingerprint enhancements processes were chosen; (1) black powder, (2) black magnetic powder, (3) red magnetic powder, (4) white powder, (5) aluminum powder and (6) cyanoacrylate fuming. For all six methods, mark enhancement was carried out before DD staining and vice versa. DD is effective in detection of DNA in the presence of both aluminum and white finger mark powders and DD does not compromise the subsequent detection of ridge patterns if DD is applied first. Whilst magnetic powders could be used to successfully enhance latent fingermarks even after DD had been applied to them, latent DNA could not be observed in the marks irrespective of whether magnetic powder was applied before or after DD treatment. Magnetic powders did not adversely affect the profiling of DNA present in the marks. The application of DD to fingermarks did not adversely affect the enhancement of fingermarks using cyanoacrylate fuming. Whilst fluorescent particles resembling cells stained with DD were observed in marks either post-treated or pre-treated with cyanoacrylate vapor, DNA amplification and profiling was not successful. While it may be important in particular investigations to collect DNA profiles from latent fingermarks with continuous ridges and clear minutiae, the main utility of the technique described here would be in relation to investigations where enhancement has resulted in only partial or smudged marks. The results presented here indicate that if it is desirable to visualize latent DNA on an object but it is also planned to treat the object with cyanoacrylate vapor or magnetic powders then it is important to apply DD first, record the location of DNA and then apply the mark enhancement technique. For aluminum and white powder mark treatments such precautions are not important.


Assuntos
Corantes , DNA/isolamento & purificação , Dermatoglifia , Cianoacrilatos , Impressões Digitais de DNA , Feminino , Humanos , Masculino , Repetições de Microssatélites , Microscopia , Reação em Cadeia da Polimerase , Pós , Tato , Volatilização
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