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1.
Artigo em Inglês | MEDLINE | ID: mdl-32578682

RESUMO

Dermatophytes are known as a common cause of superficial mycosis, but atypical presentations in immunosuppressed patients make the diagnosis more challenging. Here, we report a case of a 39-year-old patient, a renal transplant recipient from a living donor, who presented with atypical cutaneous lesions of lower extremities caused by Arthroderma gypseum (Nannizzia gypsea), four months after receiving a renal transplant. It is important to highlight the importance of the early detection of fungal infections in immunosuppressed patients. Clinicians should have a high degree of suspicion for the early detection and treatment of the cases.


Assuntos
Arthrodermataceae/isolamento & purificação , Dermatomicoses/diagnóstico , Transplante de Rim/efeitos adversos , Adulto , Dermatomicoses/patologia , Diagnóstico Diferencial , Feminino , Humanos , Hialoifomicose/diagnóstico , Hospedeiro Imunocomprometido
2.
An Bras Dermatol ; 95(3): 372-375, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32278631

RESUMO

Pseudomycetoma is an extremely rare deep mycosis, caused by dermatophytic fungi that penetrate the tissue from infected follicles of tinea capitis. Both clinically and histopathology are similar to eumycetoma, being distinguished through the isolation of the fungus, which in the case of pseudomycetoma can be Microsporum spp. or Trichophyton spp. genre. We present a 24-year-old man with an exuberant tumor in the occipital region with fistula, whose histopathological examination evidenced grains composed of hyaline hyphae and the culture for fungi isolated the agent Microsporum canis. Combined treatment of surgical excision followed by oral griseofulvin for two years was performed, with resolution of the condition.


Assuntos
Dermatomicoses/microbiologia , Microsporum/isolamento & purificação , Micetoma/microbiologia , Dermatoses do Couro Cabeludo/microbiologia , Dermatomicoses/patologia , Dermatomicoses/cirurgia , Humanos , Imunocompetência , Masculino , Micetoma/patologia , Micetoma/cirurgia , Dermatoses do Couro Cabeludo/patologia , Dermatoses do Couro Cabeludo/cirurgia , Resultado do Tratamento , Adulto Jovem
4.
J Med Microbiol ; 69(3): 436-442, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32068525

RESUMO

Introduction. Malassezia folliculitis (MF) and pityriasis versicolor (PV) are common dermatoses caused by Malassezia species. Their molecular epidemiology, drug susceptibility and exoenzymes are rarely reported in China.Aim. To investigate the molecular epidemiology, drug susceptibility and enzymatic profile of Malassezia clinical isolates.Methodology. Malassezia strains were recovered from MF and PV patients and healthy subjects (HS) and identified by sequencing analysis. The minimum inhibitory concentrations (MICs) of nine antifungals (posaconazole, voriconazole, itraconazole, fluconazole, ketoconazole, miconazole, bifonazole, terbinafine and caspofungin) and tacrolimus, the interactions between three antifungals (itraconazole, ketoconazole and terbinafine) and tacrolimus, and the extracellular enzyme profile were evaluated using broth and checkerboard microdilution and the Api-Zym system, respectively.Results. Among 392 Malassezia isolates from 729 subjects (289 MF, 218 PV and 222 HS), Malassezia furfur and Malassezia globosa accounted for 67.86 and 18.88 %, respectively. M. furfur was the major species in MF and PV patients and HS. Among 60M. furfur and 50M. globosa strains, the MICs for itraconazole, posaconazole, voriconazole and ketoconazole were <1 µg ml-1. M. furfur was more susceptible to itraconazole, terbinafine and bifonazole but tolerant to miconazole compared with M. globosa (P<0.05). Synergistic effects between terbinafine and itraconazole or between tacrolimus and itraconazole, ketoconazole or terbinafine occurred in 6, 7, 6 and 9 out of 37 strains, respectively. Phosphatases, lipases and proteases were mainly secreted in 51 isolates.Conclusions. Itraconazole, posaconazole, voriconazole and ketoconazole are theagents against which there is greatest susceptibility. Synergistic effects between terbinafine and itraconazole or tacrolimas and antifungals may be irrelevant to clinical application. Overproduction of lipases could enhance the skin inhabitation of M. furfur.


Assuntos
Antifúngicos/farmacologia , Dermatomicoses/epidemiologia , Foliculite/epidemiologia , Malassezia/isolamento & purificação , Tinha Versicolor/epidemiologia , Azóis/farmacologia , China/epidemiologia , Dermatomicoses/microbiologia , Foliculite/microbiologia , Humanos , Lipase/metabolismo , Malassezia/efeitos dos fármacos , Malassezia/enzimologia , Testes de Sensibilidade Microbiana , Epidemiologia Molecular , Pele/microbiologia , Tacrolimo/farmacologia , Terbinafina , Tinha Versicolor/microbiologia
5.
BMC Infect Dis ; 20(1): 171, 2020 Feb 22.
Artigo em Inglês | MEDLINE | ID: mdl-32087692

RESUMO

BACKGROUND: Trichophyton benhamiae is a zoophilic dermatophyte that can cause tinea in humans and animals. Lesions caused by T. benhamiae tend to be highly inflammatory, and patients are often infected by animals or other patients infected with T. benhamiae. In this paper, we report the first case of tinea faciei caused by T. benhamiae in a Chinese girl who might be transmitted from a fox. CASE PRESENTATION: A 4-year-old girl from HaiNing city developed an itchy, erythematous, and annular plaque on her right face for the past 2 months. Before the lesion appeared, she was in close contact with the fur of a fox for almost 1 week. Septate hyaline hyphae were detected by direct mycological examination of the scales. Cultures grew on Sabouraud's dextrose agar (SDA) at 26 °C for 2 weeks revealed the presence of T. mentagrophytes. A molecular sequencing test confirmed that the isolate was consistent with reference strains to T. benhamiae. Then, the diagnosis of tinea faciei due to T. benhamiae was made. Treatment with terbinafine (oral 125 mg/d) and sertaconazole nitrate cream (topical, twice daily) for 4 weeks was initiated and achieved significant improvement of the skin lesions. CONCLUSIONS: This rare dermatophytosis case highlights the importance of ITS sequencing in helping to recognize rare pathogenic fungi that can be easily misdiagnosed with a conventional morphological diagnosis.


Assuntos
Arthrodermataceae/genética , Dermatomicoses/diagnóstico , Tinha/diagnóstico , Trichophyton/genética , Administração Oral , Administração Tópica , Animais , Antifúngicos/administração & dosagem , Antifúngicos/uso terapêutico , Arthrodermataceae/isolamento & purificação , Pré-Escolar , China , Dermatomicoses/tratamento farmacológico , Dermatomicoses/microbiologia , Face/patologia , Feminino , Humanos , Imidazóis/administração & dosagem , Imidazóis/uso terapêutico , Pele/patologia , Terbinafina/administração & dosagem , Terbinafina/uso terapêutico , Tiofenos/administração & dosagem , Tiofenos/uso terapêutico , Tinha/tratamento farmacológico , Tinha/microbiologia , Resultado do Tratamento , Trichophyton/isolamento & purificação
6.
FP Essent ; 489: 16-20, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31995350

RESUMO

Fungal and viral skin infections are common and typically are managed by family physicians. The fungal skin infections commonly seen in family practice include the various forms of tinea: tinea corporis, tinea gladiatorum, tinea cruris, tinea pedis, tinea capitis, and tinea unguium (eg, onychomycosis). Common viral skin infections include herpes simplex virus infection, herpes zoster, cutaneous and genital warts, and molluscum contagiosum. Many fungal and viral skin infections have a classic appearance but variations and atypical manifestations can make patients with these conditions difficult to diagnose definitively. Confirmatory testing often is not required. In complex cases with atypical features, microscopy, culture, or blood tests can help in making a diagnosis. For some infections, treatment may be initiated before confirmatory test results are received. Most fungal skin infections can be managed effectively with topical antifungals. Tinea capitis and onychomycosis should be managed with oral drugs. Oral antiviral drugs are used to manage most viral skin infections but dosages vary based on the condition and phase of the infectious process. Cutaneous warts typically are managed with salicylic acid and/or cryotherapy.


Assuntos
Queimaduras , Dermatomicoses , Dermatopatias Infecciosas , Tinha , Queimaduras/complicações , Queimaduras/microbiologia , Dermatomicoses/diagnóstico , Dermatomicoses/tratamento farmacológico , Dermatomicoses/etiologia , Humanos , Pacientes Ambulatoriais , Dermatopatias Infecciosas/diagnóstico , Dermatopatias Infecciosas/tratamento farmacológico , Dermatopatias Infecciosas/etiologia , Tinha dos Pés
7.
Mycoses ; 63(1): 65-70, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31599024

RESUMO

Dermatomycoses, involving skin, hair and nail infections, are among the most frequent human infections with global distribution and may have a public health and economic impact. The causative agents include Dermatophytes, Candida, Malassezia and non-Dermatophyte moulds. High morbidity may be associated with certain variables: age, gender, occupation-such as farming or military service, and climate or environmental conditions. The objectives of the present study included: (a). Assessment of epidemiological aspects of dermatomycoses in the Israeli Defense Forces (IDF). (b). Antifungal drug susceptibility of fungi isolated in culture from soldiers. (a) Epidemiological assessment: data based on IDF's medical registry during the period 2009-2013 on 10 831 male and female soldiers (8164 and 2667, respectively), of which 2589 were combat soldiers. (b) Susceptibility tests: to ketoconazole, fluconazole, itraconazole, terbinafine and griseofulvin of ~ 100 Dermatophyte and Candida isolates, using E test and/or disc diffusion assays. (c) Statistical analysis: logistic regression, chi-square and ANOVA. (a) Incidence in male soldiers higher than in female soldiers (35% vs. 28%). (b) Incidence in combat soldiers higher than in non-combat soldiers (39% vs 32%). (c) The major site of involvement-nails. (d) Infections peaked during summer months. (e) Dermatophytes constitute close to 90% of the aetiological agents (87% and 86%). (f) Trichophyton rubrum the dominant species. (g) terbinafine was the most active antifungal drug. The most significant conclusion of relevance of this study is the finding of higher morbidity rate among combat soldiers, as this may affect the activity of this group.


Assuntos
Antifúngicos/uso terapêutico , Arthrodermataceae/isolamento & purificação , Dermatomicoses , Terbinafina/uso terapêutico , Trichophyton/isolamento & purificação , Adulto , Arthrodermataceae/efeitos dos fármacos , Candida/efeitos dos fármacos , Candida/isolamento & purificação , Estudos de Coortes , Dermatomicoses/tratamento farmacológico , Dermatomicoses/epidemiologia , Dermatomicoses/microbiologia , Dermatomicoses/patologia , Feminino , Humanos , Israel/epidemiologia , Masculino , Testes de Sensibilidade Microbiana , Saúde Militar , Militares , Estações do Ano , Trichophyton/efeitos dos fármacos
8.
J Appl Microbiol ; 128(4): 1038-1049, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31816165

RESUMO

AIM: The purpose of this study was to uncover insights into the mechanism of action of the 8-hydroxyquinoline derivatives PH151 and PH153. In addition, with the future perspective of developing a topical drug for the treatment of candidiasis and dermatophytosis, the antifungal activity of a nanoemulsion formulation containing the most active compound (PH151) is also presented here. METHODS AND RESULTS: Sorbitol protection assay and scanning electron microscopy indicate that the 8-hydroxyquinoline derivatives act on the cell wall of Candida sp. and dermatophytes and they inhibit the pseudohyphae formation of C. albicans. These findings demonstrate a strong effect of these compounds on C. albicans morphogenesis, which can be considered a potential mode of action for this molecule. Besides, the nanoemulsion formulation MIC values ranged from 0·5 to 4 µg ml-1 demonstrating the significant antifungal activity when incorporated into a pharmaceutical formulation. CONCLUSIONS: Taken together, the results support the potential of these molecules as promising antifungal candidates for the treatment of candidiasis and dermatophytosis. SIGNIFICANCE AND IMPACT OF THE STUDY: There is an emerging need to fill the pipeline with new antifungal drugs due to the limitations presented by the currently used drugs. In this study, we have described a novel formulation with a 8-hydroxyquinoline-5-sulfonamide derivative which has presented a great potency in providing a finished product. Furthermore, the derivative has shown a selective mechanism of action confirming its potential to be developed into a new drug candidate.


Assuntos
Antifúngicos/farmacologia , Arthrodermataceae/efeitos dos fármacos , Dermatomicoses/microbiologia , Oxiquinolina/farmacologia , Sulfonamidas/farmacologia , Antifúngicos/química , Arthrodermataceae/crescimento & desenvolvimento , Candida albicans/efeitos dos fármacos , Candida albicans/crescimento & desenvolvimento , Candidíase/tratamento farmacológico , Candidíase/microbiologia , Parede Celular/efeitos dos fármacos , Dermatomicoses/tratamento farmacológico , Hifas/efeitos dos fármacos , Hifas/crescimento & desenvolvimento , Testes de Sensibilidade Microbiana , Oxiquinolina/química , Sulfonamidas/química
9.
Mycoses ; 63(1): 21-29, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31610041

RESUMO

BACKGROUND: Despite the worldwide prevalence of dermatophyte infections, only a few genes are reported to be related to dermatophyte infections. In addition, the mechanism by which different ecological dermatophytes infection leads to varying intensity of inflammation remains unclear. OBJECTIVES: To investigate the mechanism of varying intensity of skin inflammation caused by different ecological dermatophytes infection. METHODS: We infected HaCaT cells with anthropophilic and geophilic dermatophytes to mimic various ecological dermatophyte infections. RNA-sequencing (RNA-seq) was employed to identify the change in the gene expression of HaCaT cells. To verify the expression of differentially expressed genes (DEGs), we selected 18 HaCaT cells genes to conduct qPCR experiments. In addition, immunoblotting was conducted to validate key genes from the MAPK signalling pathway. RESULTS: After HaCaT cells were infected with the anthropophilic Trichophyton rubrum (T rubrum) and the geophilic Microsporum gypseum (M gypseum), 118 and 619 differentially expressed genes were identified in HaCaT cells, respectively. These genes may provide a clue as to how keratinocytes respond to anthropophilic and geophilic dermatophytes. We also found that JUN may play a critical role in keratinocytes infected with M gypseum. CONCLUSIONS: Differential gene expression in HaCaT cells may account for the various clinical presentation caused by anthropophilic and geophilic dermatophytes infections. In addition, the intense inflammatory reaction of M gypseum infection may be triggered by activating the JNK-JUN signalling pathway.


Assuntos
Arthrodermataceae , Interações Hospedeiro-Patógeno/imunologia , Queratinócitos/metabolismo , Queratinócitos/microbiologia , Arthrodermataceae/patogenicidade , Linhagem Celular , Dermatomicoses/genética , Dermatomicoses/imunologia , Dermatomicoses/microbiologia , Perfilação da Expressão Gênica , Humanos , Queratinócitos/imunologia , Microsporum/patogenicidade , Transdução de Sinais/genética , Trichophyton/patogenicidade
10.
Med Mycol J ; 60(4): 101-107, 2019.
Artigo em Japonês | MEDLINE | ID: mdl-31787730

RESUMO

Cutaneous cryptococcosis is classified either as primary or secondary based on the route of infection. The disease can also be classified either as localized cutaneous cryptococcosis or cutaneous manifestations of disseminated cryptococcosis. However, from a physician's point of view, whether lesions are localized to the skin or are disseminated/systemic is more important than the route of infection. The Clinical Practice Guidelines for Diagnosis and Treatment of Cryptococcosis, which was established in 2019 by the Japanese Society for Medical Mycology, adopted the latter classification. Localized cutaneous cryptococcosis is defined as a condition in which lesions are confined within a limited part of the skin, not systemically disseminated at the same time, and are associated with neither cryptococcal fungemia nor antigenemia. This type of cutaneous cryptococcosis is uncommon in Japan. Only 65 cases were reported during the 50-year study period from 1968 to August 2018, with the patients divided into two groups: immunocompromised patients (n=44, 68%) and immunocompetent patients (n=21, 32%). None of the patients were infected with the human immunodeficiency virus (HIV). Localized cutaneous cryptococcosis can also occur in non-HIV-infected patients and well-appearing individuals, therefore, it is considered an important infection in routine dermatology practice. Here, we outline the classification, diagnosis, and treatment of cutaneous cryptococcosis and present a summary of cutaneous cryptococcosis cases reported in Japan.


Assuntos
Criptococose , Dermatomicoses , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Criptococose/classificação , Criptococose/diagnóstico , Criptococose/tratamento farmacológico , Criptococose/patologia , Dermatomicoses/classificação , Dermatomicoses/diagnóstico , Dermatomicoses/tratamento farmacológico , Dermatomicoses/patologia , Feminino , Humanos , Imunocompetência , Hospedeiro Imunocomprometido , Masculino , Pessoa de Meia-Idade
11.
An Bras Dermatol ; 94(6): 744-746, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31789256

RESUMO

This report describes a case of unusual deep skin ulcers with tortuous sinus tract formation in an immunocompetent woman. She was initially diagnosed with a Staphylococcus aureus skin infection and histopathologically diagnosed with pyoderma gangrenosum. However, culture from the deep end of ribbon gauze inserted into the subcutaneous sinus tract revealed shiny, light-yellow mucoid colonies, which were identified as Cryptococcus neoformans var. grubii. She was treated with fluconazole for nine months and completely healed. Cryptococcosis is an opportunistic infection caused by variants of C. neoformans species. Cutaneous manifestations of cryptococcosis are quite divergent, rarely occurring as deep skin ulcers with sinus formation.


Assuntos
Criptococose/patologia , Cryptococcus neoformans/isolamento & purificação , Dermatomicoses/patologia , Imunocompetência , Úlcera Cutânea/microbiologia , Úlcera Cutânea/patologia , Adulto , Antifúngicos/uso terapêutico , Criptococose/tratamento farmacológico , Criptococose/microbiologia , Dermatomicoses/tratamento farmacológico , Dermatomicoses/microbiologia , Feminino , Fluconazol/uso terapêutico , Humanos , Hospedeiro Imunocomprometido , Úlcera Cutânea/tratamento farmacológico
13.
PLoS One ; 14(12): e0225796, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31851674

RESUMO

Dandruff is a skin condition that affects the scalp of up to half the world's population, it is characterised by an itchy, flaky scalp and is associated with colonisation of the skin by Malassezia spp. Management of this condition is typically via antifungal therapies, however the precise role of microbes in the aggravation of the condition are incompletely characterised. Here, a combination of 454 sequencing and qPCR techniques were used to compare the scalp microbiota of dandruff and non-dandruff affected Chinese subjects. Based on 454 sequencing of the scalp microbiome, the two most abundant bacterial genera found on the scalp surface were Cutibacterium (formerly Propionibacterium) and Staphylococcus, while Malassezia was the main fungal inhabitant. Quantitative PCR (qPCR) analysis of four scalp taxa (M. restricta, M. globosa, C. acnes and Staphylococcus spp.) believed to represent the bulk of the overall population was additionally carried out. Metataxonomic and qPCR analyses were performed on healthy and lesional buffer scrub samples to facilitate assessment of whether the scalp condition is associated with differential microbial communities on the sampled skin. Dandruff was associated with greater frequencies of M. restricta and Staphylococcus spp. compared with the healthy population (p<0.05). Analysis also revealed the presence of an unclassified fungal taxon that could represent a novel Malassezia species.


Assuntos
Caspa , Dermatomicoses/microbiologia , Malassezia , Microbiota , Couro Cabeludo/microbiologia , Pele/microbiologia , Adolescente , Adulto , Idoso , China , Caspa/epidemiologia , Caspa/microbiologia , Feminino , Humanos , Malassezia/classificação , Malassezia/isolamento & purificação , Masculino , Pessoa de Meia-Idade , Propionibacteriaceae/isolamento & purificação , Staphylococcus/isolamento & purificação , Adulto Jovem
14.
Enferm. infecc. microbiol. clín. (Ed. impr.) ; 37(10): 642-647, dic. 2019. tab, graf
Artigo em Inglês | IBECS | ID: ibc-189590

RESUMO

OBJECTIVE: The aim of this study was to evaluate the epidemiological profile of dermatophytoses from 2008 to 2017 in the area of "Barcelonès Nord", located in north-eastern Spain. METHODS: From 2008 to 2017, 13,419 specimens obtained from patients with suspected superficial mycosis were subjected to direct microscopy and culture. Clinical and sociodemographic data were recorded. Proportions were compared using Chi-square and Fisher's exact tests. A logistic regression model was used for multivariate analysis. RESULTS: Trichophyton rubrum was the most frequently isolated fungus (76.1%), followed by Trichophyton mentagrophytes/Trichophyton interdigitale (11.9%) and Microsporum canis (2.9%). Among the population over 15 years of age, tinea unguium pedum (40.4%) and tinea corporis (29.1%) were the predominant dermatophyte infections. Tinea capitis was mostly prevalent (53.6%) among patients up to 15 years of age, followed by tinea corporis (21.4%). We observed an increase in non-endemic anthropophilic dermatophytes (Trichophyton soudanense, Microsporum audouinii and Trichophyton violaceum) in the last few years. These species were associated with the population up to 15 years of age (p < 0.001), having tinea capitis (p = 0.0017) and being of African/Hindustani origin (p < 0.001). CONCLUSIONS: We confirmed the spread of T. rubrum as the predominant dermatophyte in our area and reported an increase in non-endemic anthropophilic dermatophytes in the last few years, especially in the African and Hindustani population up to 15 years of age


OBJETIVO: La finalidad del presente estudio fue evaluar el perfil epidemiológico de las dermatofitosis entre los años 2008 y 2017 en el área de «Barcelonès Nord» ubicada en el noreste de España. MÉTODOS: Entre los años 2008 y 2017, 13.419 muestras obtenidas de pacientes con sospecha de micosis superficial fueron remitidas para microscopía directa y cultivo. Se registraron datos clínicos y sociodemográficos. Las proporciones se compararon mediante las pruebas de Chi-cuadrado y Fisher. Para el análisis de variables múltiples, se utilizó un modelo de regresión logística. RESULTADOS: Trichophyton rubrum fue el hongo más frecuentemente aislado (76,1%), seguido de Trichophyton mentagrophytes/Trichophyton interdigitale (11,9%) y Microsporum canis (2,9%). Entre la población mayor de 15 años, la tinea unguium pedum (40,4%) y la tinea corporis (29,1%) fueron las dermatofitosis predominantes. La tinea capitis prevaleció principalmente (53,6%) entre los pacientes menores de 15 años, seguida de la tinea corporis (21,4%). Se percibió un aumento de los dermatofitos antropófilos no endémicos (Trichophyton soudanense, Microsporum audouinii y Trichophyton violaceum) en los últimos años. Estas especies se asociaron con la población menor de 15 años (p < 0,001), la presencia de tinea capitis (p = 0,0017) y el origen africano/indostánico (p < 0,001). CONCLUSIONES: Confirmamos el predominio de Trichophyton rubrum como el dermatofito más prevalente en nuestra área, y describimos un aumento en los dermatofitos antropófilos no endémicos en los últimos años, especialmente en población africana e indostana menor de 15 años


Assuntos
Humanos , Tinha/epidemiologia , Tinha/microbiologia , Dermatomicoses/epidemiologia , Dermatomicoses/microbiologia , Espanha/epidemiologia , Modelos Logísticos , Trichophyton/isolamento & purificação
16.
PLoS One ; 14(11): e0225224, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31725762

RESUMO

The largest current disease-induced loss of vertebrate biodiversity is due to chytridiomycosis and despite the increasing understanding of the pathogenesis, knowledge unravelling the early host-pathogen interactions remains limited. Batrachochytrium dendrobatidis (Bd) zoospores attach to and invade the amphibian epidermis, with subsequent invasive growth in the host skin. Availability of an in vitro assay would facilitate in depth study of this interaction while reducing the number of experimental animals needed. We describe a fluorescent cell-based in vitro infection model that reproduces host-Bd interactions. Using primary keratinocytes from Litoria caerulea and the epithelial cell line A6 from Xenopus laevis, we reproduced different stages of host cell infection and intracellular growth of Bd, resulting in host cell death, a key event in chytridiomycosis. The presented in vitro models may facilitate future mechanistic studies of host susceptibility and pathogen virulence.


Assuntos
Anfíbios/microbiologia , Quitridiomicetos/fisiologia , Doenças dos Animais/microbiologia , Animais , Linhagem Celular , Células Cultivadas , Dermatomicoses/veterinária
18.
An. bras. dermatol ; 94(6): 744-746, Nov.-Dec. 2019. graf
Artigo em Inglês | LILACS | ID: biblio-1054899

RESUMO

Abstract This report describes a case of unusual deep skin ulcers with tortuous sinus tract formation in an immunocompetent woman. She was initially diagnosed with a Staphylococcus aureus skin infection and histopathologically diagnosed with pyoderma gangrenosum. However, culture from the deep end of ribbon gauze inserted into the subcutaneous sinus tract revealed shiny, light-yellow mucoid colonies, which were identified as Cryptococcus neoformans var. grubii. She was treated with fluconazole for nine months and completely healed. Cryptococcosis is an opportunistic infection caused by variants of C. neoformans species. Cutaneous manifestations of cryptococcosis are quite divergent, rarely occurring as deep skin ulcers with sinus formation.


Assuntos
Humanos , Feminino , Adulto , Úlcera Cutânea/microbiologia , Úlcera Cutânea/patologia , Criptococose/patologia , Cryptococcus neoformans/isolamento & purificação , Dermatomicoses/patologia , Imunocompetência , Úlcera Cutânea/tratamento farmacológico , Fluconazol/uso terapêutico , Hospedeiro Imunocomprometido , Criptococose/microbiologia , Criptococose/tratamento farmacológico , Dermatomicoses/microbiologia , Dermatomicoses/tratamento farmacológico , Antifúngicos/uso terapêutico
20.
Dermatology ; 235(6): 495-500, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31586992

RESUMO

The prevalence of skin disease varies with geographic location and may be affected by cultural factors. In Haiti, the combination of poor health care access, infrastructure lack, and natural disasters may affect the prevalence of skin conditions. Unfortunately, little epidemiological information has been obtained on the prevalence of skin diseases in Haiti. The objective of this study is to describe data on the prevalence of dermatological conditions in urban areas of Haiti. Univariate, bivariate, and χ2 analyses were used to evaluate associations. During February 2016, we documented 137 patients. The mean age was 27.7 years; most of the subjects were women (61.3%) in a cohort mostly adult (67.1%). The infectious and inflammatory dermatoses were the most prevalent (74.2%). Infectious skin diseases were more prevalent in men and in adults in general (p < 0.01). Inflammatory conditions were also more common in adults (p < 0.05). Additionally, alopecia and dyschromia were limited to women (p < 0.01). Infectious skin diseases, in particular fungal infections, were amongst the most common diagnoses in the urban area of Haiti. Therefore, improved access to antimicrobial and anti-inflammatory medications would likely benefit Haitian patients with dermatological conditions. These findings may help local public health officials and future dermatological expeditions better serve this population.


Assuntos
Dermatopatias/epidemiologia , População Urbana/estatística & dados numéricos , Adolescente , Adulto , Alopecia/epidemiologia , Criança , Estudos Transversais , Dermatite/epidemiologia , Dermatomicoses/epidemiologia , Feminino , Haiti/epidemiologia , Humanos , Masculino , Transtornos da Pigmentação/epidemiologia , Prevalência , Fatores Sexuais , Adulto Jovem
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