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1.
Niger J Clin Pract ; 22(12): 1758-1764, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31793485

RESUMO

Aim: The aim of this in vitro study was to compare the effects of the application of three different cavity disinfecting agents to dentin on the micro-shear bond strength (µ-SBS) of one self-etch and two universal adhesive systems. Materials and Methods: In total, 120 caries-free human permanent molar teeth were used in this study. Mid-coronal dentin surfaces were revealed by cutting occlusal enamel and a standard smear layer was obtained by using 600-800-1200 grid silicon carbide abrasive papers. Specimens were randomly assigned to four groups according to the disinfectant used: Group 1: Control (no disinfectant); Group 2: 2% chlorhexidine based (Consepsis); Group 3: 10 ppm ozonated water (TeknO3zone); Group 4: 5% boric acid (Handmade). Each group was divided into three subgroups according to the type of adhesive (Clearfil SE Bond, OptiBond XTR, and Tokuyama Universal). Specimens were bonded using either Clearfil SE Bond, OptiBond XTR or Tokuyama Universal, which were employed according to the manufacturer's instructions. Resin composite microcylinders were bonded using Tygon® tubes for µ-SBS testing. After specimens were stored for 24 h, at 37°C in distilled water, µ-SBS test was measured with a universal test machine (LF Plus, Lloyd, Instrument). µ-SBS results were analyzed by one-way analysis of variance (ANOVA) and Tukey's tests. Results: When the mean microshear bond strength values of the control group were compared, the difference between the subgroups was not significant (P < 0.05). When the mean microshear bond strength values of the chx, ozonated water, and boric acid were compared, the difference between Clearfil SE Bond and Tokuyama Universal was significant (P < 0.05) and the difference between the other groups was not significant (P > 0.05). Conclusion: Ozonated water and boric acid may be as an alternative to other materials used as cavity disinfectants.


Assuntos
Ácidos Bóricos , Colagem Dentária/métodos , Cimentos Dentários , Desinfetantes de Equipamento Odontológico/farmacologia , Adesivos Dentinários , Dentina/efeitos dos fármacos , Água , Clorexidina , Resinas Compostas/química , Esmalte Dentário , Corrosão Dentária/métodos , Humanos , Cimentos de Resina/uso terapêutico , Camada de Esfregaço
2.
Med Sci Monit ; 25: 2908-2916, 2019 Apr 20.
Artigo em Inglês | MEDLINE | ID: mdl-31004424

RESUMO

BACKGROUND The aim of this research was to systematically analyze the effectiveness of calcium hydroxide compared to formocresol (FC) and camphor phenol (CP) in root canal disinfection of primary teeth. MATERIAL AND METHODS The meta-analysis was based on the participants, interventions, control, outcome (PICO) study design principle and 16 randomized-controlled clinical trials published from January 2000 to August 2018. The data heterogeneity of each study was assessed by the Q-test. The odds ratio and 95% confidence interval (CI) were calculated based on the heterogeneity results by Revman software. RESULTS Sixteen randomized-controlled clinical trials of 3047 primary teeth were included in this meta-analysis. There were significant differences of clinical effectiveness between calcium hydroxide and FC in root canal disinfection of primary teeth (OR=3.37; 95% CI range: 2.54-4.48, P<0.01) and endodontic inter-appointment emergencies (EIAE) after disinfection for 7 days (OR=0.26; 95% CI range: 0.16-0.42, P<0.01). However, there was no statistical difference of EIAE, after disinfection of primary teeth for 48 hours, between calcium hydroxide and FC (OR=0.62; 95% CI range: 0.34-1.11, P=0.11). There were significant differences of clinical effectiveness between the calcium hydroxide and CP in root canal disinfection of primary teeth (OR=5.50; 95% CI range: 3.36-8.98, P<0.01). CONCLUSIONS This meta-analysis indicated that the effectiveness of calcium hydroxide as root canal disinfectant in primary teeth was more effective than that of FC and CP.


Assuntos
Hidróxido de Cálcio/uso terapêutico , Desinfetantes de Equipamento Odontológico/uso terapêutico , Tratamento do Canal Radicular/métodos , Cânfora/uso terapêutico , Cavidade Pulpar/efeitos dos fármacos , Combinação de Medicamentos , Formocresóis/uso terapêutico , Humanos , Fenóis/uso terapêutico , Ensaios Clínicos Controlados Aleatórios como Assunto , Dente Decíduo/cirurgia , Resultado do Tratamento
3.
Clin Oral Investig ; 23(10): 3789-3799, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30759283

RESUMO

OBJECTIVES: The aim of this randomized, three-arm parallel, single-blinded clinical trial was to evaluate the clinical and microbiological effectiveness of the ozone application in two-visit indirect pulp therapy. MATERIALS AND METHODS: One hundred five lower first molar teeth with deep caries lesion were included and randomly assigned three groups to apply the two-visit indirect pulp therapy. Treatment procedure was applied without any disinfectant (control), with 60-s 2% chlorhexidine digluconate (CHX) or 60-s ozone application. In four different stages (after initial excavation, ozone/CHX application before the temporary restoration, 4 months later immediately after removing temporary restoration, and final excavation), dentin humidity, consistency, and color properties were recorded to evaluate the clinical characteristics of the tissue, and standard dentin samples were collected for the microbiological analysis of mutans streptococci, lactobacilli, and the total number of colony-forming units. The data were analyzed by using Mann-Whitney U test for multiple comparisons. RESULTS: The remaining dentin became harder, drier, and darker after 4 months in all groups. However, CHX and ozone application were statistically better than the control group (p < 0.05). There was a gradual decrease in the total number of microorganisms in all groups. While cavity disinfectant applications were improved the antibacterial efficacy (control, 79.11%; CHX, 98.39%; ozone, 93.33%), CHX application exhibited a greater significant reduction than both groups (p = 0.000). CONCLUSION: The two-visit indirect pulp therapy yielded successful results for all study groups. However, CHX would be conveniently preferable due to improving the treatment success. CLINICAL RELEVANCE: The two-visit indirect pulp therapy applied with cavity disinfectant is a proper alternative treatment procedure in deep carious lesions, instead of conventional technique.


Assuntos
Cárie Dentária/terapia , Desinfetantes de Equipamento Odontológico/uso terapêutico , Dente Molar/patologia , Ozônio/uso terapêutico , Exposição da Polpa Dentária , Dentina , Humanos , Streptococcus mutans
4.
J Pharm Biomed Anal ; 164: 360-364, 2019 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-30439663

RESUMO

Accurate knowledge of the safety of the combined use of hyper-pure chlorine dioxide (ClO2, Solumium) with different endodontic agents is not known. The objective was to investigate the possible interactions between hyper-pure ClO2 and Chlorhexidine digluconate (CHX) as well as hyper-pure ClO2 and ethylenediaminetetraacetic acid (EDTA) in vitro. High-performance liquid chromatography (HPLC) was used to analyse the reaction between ClO2 and CHX for studying the possible increase of para-chloroaniline (PCA). Nuclear magnetic resonance (NMR) method was applied to investigate if the oxidising property of ClO2 inactivates EDTA. CHX itself may contain PCA; however, its amount did not increase after the mixing up with ClO2. While ClO2 did not oxidise PCA, on the other hand, it oxidised EDTA into numerous degradation products, but this reaction was slow. Hyper-pure ClO2 of excellent antimicrobial properties should be considered as a safe alternative endodontic disinfectant agent in combinations or as a final rinse. The lack of chemical interaction between ClO2 and CHX confirms their safe application in combination.


Assuntos
Anti-Infecciosos Locais/química , Clorexidina/análogos & derivados , Compostos Clorados/química , Desinfetantes de Equipamento Odontológico/química , Ácido Edético/química , Óxidos/química , Anti-Infecciosos Locais/efeitos adversos , Anti-Infecciosos Locais/farmacologia , Clorexidina/efeitos adversos , Clorexidina/química , Compostos Clorados/efeitos adversos , Compostos Clorados/farmacologia , Cromatografia Líquida de Alta Pressão/instrumentação , Cromatografia Líquida de Alta Pressão/métodos , Desinfetantes de Equipamento Odontológico/efeitos adversos , Desinfetantes de Equipamento Odontológico/farmacologia , Cavidade Pulpar/efeitos dos fármacos , Desinfecção/métodos , Combinação de Medicamentos , Interações de Medicamentos , Ácido Edético/efeitos adversos , Humanos , Oxirredução , Óxidos/efeitos adversos , Óxidos/farmacologia , Espectroscopia de Prótons por Ressonância Magnética/métodos
5.
J Endod ; 45(1): 31-44.e13, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30558797

RESUMO

INTRODUCTION: The aim of this study was to systematically review the evidence on the cleaning and disinfection of root canals and the healing of apical periodontitis when ultrasonic irrigant activation is applied during primary root canal treatment of mature permanent teeth compared with syringe irrigation. METHODS: An electronic search was conducted of the Cochrane Library, Embase, LILACS, PubMed, SciELO, and Scopus databases using both free-text key words and controlled vocabulary. Additional studies were sought through hand searching of endodontic journals and textbooks. The retrieved studies were screened by 2 reviewers according to predefined criteria. The included studies were critically appraised, and the extracted data were arranged in tables. RESULTS: The electronic and hand search retrieved 1966 titles. Three clinical studies and 45 in vitro studies were included in this review. Ultrasonic activation did not improve the healing rate of apical periodontitis compared with syringe irrigation after primary root canal treatment of teeth with a single root canal. Conflicting results were reported by the in vitro microbiological studies. Ultrasonic activation was more effective than syringe irrigation in the removal of pulp tissue remnants and hard tissue debris based on both clinical and in vitro studies. Ultrasonic activation groups were possibly favored in 13 studies, whereas syringe irrigation groups may have been favored in 3 studies. CONCLUSIONS: The level of the available evidence was low, so no strong clinical recommendations could be formulated. Future studies should focus on the antimicrobial effect and healing of apical periodontitis in teeth with multiple root canals.


Assuntos
Desinfetantes de Equipamento Odontológico/administração & dosagem , Cavidade Pulpar , Detergentes/administração & dosagem , Periodontite Periapical/terapia , Irrigantes do Canal Radicular/administração & dosagem , Tratamento do Canal Radicular , Seringas , Irrigação Terapêutica/métodos , Ultrassom , Bases de Dados Bibliográficas , Humanos , Periodontite Periapical/fisiopatologia , Resultado do Tratamento
6.
Wiad Lek ; 71(4): 855-860, 2018.
Artigo em Russo | MEDLINE | ID: mdl-30099423

RESUMO

OBJECTIVE: Introduction: Periodontal diseases take second place in the world among dental diseases and tend to be ubiquitous regardless of age, sex and place of residence. According to the modern point of view inflammatory periodontal diseases refer to infectious chronic inflammatory diseases, therefore, the normalization of the microflora of the oral cavity is an essential condition for their rational therapy. The aim: To increase the effectiveness of conservative treatment of chronic generalized periodontitis of the II-nd and III-rd severity using the medication based on the antiseptic Troklosene. PATIENTS AND METHODS: Materials and methods: 34 patients of age 45 to 55 years who were diagnosed with chronic generalized periodontitis of the IIInd and IIIrd degree of severity were examined and treated. All patients reported signs of the disease for 3-15 years. Patients of the study group by simple randomization were divided into two subgroups, depending on the therapeutic treatment performed. The I-st subgroup -16 patients treated with the additional use of the medication based on the antiseptic Troklosene, the IInd subgroup - 18 patients who were treated without using this drug. RESULTS: Results: Analyzing the results after the treatment in the patients of the I -st and II -nd subgroup, they noted a decrease in PMA index, Green-Vermillion index, bleeding index. Pathological mobility of teeth decreased to the I degree. The hygienic condition of the oral cavity was characterized in patients of both subgroups after treatment as satisfactory. In patients of the Ist subgroup (with the additional use of the medication based on the antiseptic Troklosene), after the 5th visit there were signs of remission, and in the IInd subgroup (using standard base therapy), after 7 visits, there were signs of clinical remission. With the additional use of the drug based on the antiseptic Troklosene, in contrast to the traditional therapy, a change in the bacterial ratio of microorganisms occurred, which influenced a significant decrease in the total bacterial mass. CONCLUSION: Conclusion: The best results were obtained in the Ist subgroup, where along with the standard base therapy, the supplement of based on the antiseptic Troklosene was used additionally. Introduction to the treatment regimen for patients with chronic generalized periodontitis with II and III severity of the drug based on the antiseptic Troklosene contributed to a stable clinical effect, as evidenced by the dynamics of clinical indicators and the reduction in the number of visits. Also, a change in the bacterial ratio of microorganisms with a significant decrease due to Enterobacterium spp. is noted. To maintain this result, it is recommended to continue the use of the drug based on the antiseptic Troklosene with a frequency of 4 times per day within 7 days after the end of treatment.


Assuntos
Anti-Infecciosos Locais/uso terapêutico , Desinfetantes de Equipamento Odontológico/uso terapêutico , Profilaxia Dentária/métodos , Índice Periodontal , Periodontite/diagnóstico , Periodontite/terapia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Resultado do Tratamento
7.
J Endod ; 44(10): 1474-1479, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30144986

RESUMO

INTRODUCTION: This randomized clinical study compared the antibacterial effectiveness of treatment protocols using either a triple antibiotic solution (1 mg/mL) or calcium hydroxide/chlorhexidine paste as interappointment medication in infected canals of teeth with primary apical periodontitis. METHODS: The root canals of single-rooted teeth with apical periodontitis were prepared by using a reciprocating single-instrument technique with 2.5% sodium hypochlorite irrigation and then medicated for 1 week with either a triple antibiotic solution (minocycline, metronidazole, and ciprofloxacin) at 1 mg/mL (n = 24) or a calcium hydroxide paste in 2% chlorhexidine gluconate (n = 23). Samples were taken from the canal at the baseline (S1), after chemomechanical preparation (S2), and after intracanal medication (S3). DNA extracts from clinical samples were evaluated for total bacterial reduction using a 16S ribosomal RNA gene-based quantitative polymerase chain reaction assay. RESULTS: All S1 samples were positive for the presence of bacteria, and counts were substantially reduced after treatment procedures (P < .01). Bacterial levels in S2 and S3 samples did not significantly differ between groups (P > .05). S2 to S3 reduction was 97% in the antibiotic group and 39% in the calcium hydroxide/chlorhexidine group; only the former reached statistical significance (P < .01). There were significantly more quantitative polymerase chain reaction-negative S3 samples in the antibiotic group than in the calcium hydroxide group (P < .05). CONCLUSIONS: Interappointment medication with a triple antibiotic solution at the concentration of 1 mg/mL significantly improved root canal disinfection, and its effects were at least comparable with the calcium hydroxide/chlorhexidine paste. Effectiveness and easy delivery of the antibiotic solution make it an appropriate medicament as part of a disinfecting protocol for conventional nonsurgical endodontic treatment and possibly regenerative endodontic procedures.


Assuntos
Antibacterianos/administração & dosagem , Infecções Bacterianas/microbiologia , Infecções Bacterianas/prevenção & controle , Hidróxido de Cálcio/administração & dosagem , Clorexidina/administração & dosagem , Ciprofloxacino/administração & dosagem , Desinfetantes de Equipamento Odontológico/administração & dosagem , Metronidazol/administração & dosagem , Minociclina/administração & dosagem , Periodontite Periapical/microbiologia , Periodontite Periapical/cirurgia , Irrigantes do Canal Radicular/administração & dosagem , Preparo de Canal Radicular/métodos , Adolescente , Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Pomadas , Soluções , Adulto Jovem
8.
BMC Complement Altern Med ; 18(1): 219, 2018 Jul 18.
Artigo em Inglês | MEDLINE | ID: mdl-30021632

RESUMO

BACKGROUND: Propolis is a natural substance produced by bees and is known to have antimicrobial activity. Our aim was to evaluate the antimicrobial effect of micellar nanocomposites loaded with an ethyl acetate extract of Brazilian red propolis as a cavity cleaning agent and its influence on the color and microtensile bond strength (µTBS) of the dentin/resin interface. METHODS: An ultra-performance liquid chromatography coupled with a diode array detector (UPLC-DAD) assay was used to determine the flavonoids and isoflavones present in an ethyl acetate extract of Brazilian red propolis (EARP) and micellar nanocomposites loaded with EARP (MNRP). The antimicrobial activity of EARP and MNRP was tested against Streptococcus mutans, Lactobacillus acidophilus, and Candida albicans. One of the following experimental treatments was applied to etched dentin (phosphoric acid, 15 s): 5 µL of MNRP (RP3, 0.3%; RP6, 0.6%; or RP1, 1.0% w/v), placebo, and 2% chlorhexidine digluconate. Single Bond adhesive (3 M/ESPE) was applied and a 4-mm-thick resin crown (Z350XT, 3 M/ESPE) was built up. After 24 h, the teeth were sectioned into sticks for the µTBS test and scanning electron microscopy. Spectrophotometry according to the CIE L*a*b* chromatic space was used to evaluate the color. Data were analyzed using one-way ANOVA and the Tukey test or Kruskal-Wallis test and the same test for pairwise comparisons between the means (P < 0.05). RESULTS: The UPLC-DAD assay identified the flavonoids liquiritigenin, pinobanksin, pinocembrin, and isoliquiritigenin and the isoflavonoids daidzein, formononetin, and biochanin A in the EARP and micellar nanocomposites. EARP and MNRP presented antimicrobial activity against the cariogenic bacteria Streptococcus mutans and Lactobacillus acidophilus, and for Candida albicans. ΔE values varied from 2.31 to 3.67 (P = 0.457). The mean µTBS for RP1 was significantly lower than for the other groups (P < 0.001). Dentin treated with RP1 showed the shortest resin tags followed by RP6 and RP3. CONCLUSIONS: The EARP and (MNRP) showed antimicrobial activity for the main agents causing dental caries (Streptococcus mutans and Lactobacillus acidophilus) and for Candida albicans. MNRP at concentrations of 0.3 and 0.6% used as a cavity cleaner do not compromise the aesthetics or µTBS of the dentin/resin interface.


Assuntos
Desinfetantes de Equipamento Odontológico , Nanocompostos/química , Extratos Vegetais , Própole/química , Antibacterianos/química , Antibacterianos/farmacologia , Bactérias/efeitos dos fármacos , Candida/efeitos dos fármacos , Desinfetantes de Equipamento Odontológico/química , Desinfetantes de Equipamento Odontológico/farmacologia , Flavonoides , Teste de Materiais , Micelas , Testes de Sensibilidade Microbiana , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Resinas Sintéticas , Estresse Mecânico , Resistência à Tração
9.
Dent Mater J ; 37(5): 740-745, 2018 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-29848854

RESUMO

Infection control of dental stone cast is an important issue. Ozone is effective for disinfection against microorganisms and inactivation of viruses. However, there is little information regarding the use of ozone. We prepared 4 types of gypsum specimens and 3 types of disinfectants (4-5 ppm Ozonated water [OZW], 2% glutaraldehyde [GL], and 1% sodium hypochlorite [SH]). Gypsum specimens were immersed in each disinfectant for 5 and 10 min, and surface roughness was then examined using laser scanning microscopy. Surface microstructure was investigated using scanning electron microscopy. Immersion of gypsum specimens in SH, GL, and OZW increased the surface roughness to a maximum of 1.04, 0.37, and 0.30 µm, respectively, based on the difference between the average values of surface roughness before and after the disinfection procedure. The effects of OZW and GL were comparable. OZW is useful as a candidate for relatively safe disinfection of material for dental stone casts.


Assuntos
Sulfato de Cálcio/química , Revestimento para Fundição Odontológica/química , Desinfetantes de Equipamento Odontológico/química , Materiais para Moldagem Odontológica/química , Ozônio/química , Água/química , Glutaral/química , Teste de Materiais , Microscopia Eletrônica de Varredura , Hipoclorito de Sódio/química , Propriedades de Superfície
10.
Rev. Eugenio Espejo ; 12(1): 44-52, Jun.- 2018.
Artigo em Espanhol | LILACS | ID: biblio-980678

RESUMO

El presente estudio comprende el análisis y la eliminación de los microorganismos presentes en los sistemas de irrigación de las unidades dentales de la Universidad Nacional de Chimbo-razo, el mismo tiene como finalidad la reducción de contaminación microbiana. Se procedió a tomar muestras del agua del sistema de irrigación que recorre por la jeringa triple de diez unidades dentales escogidas al azar, la recolección se realizó en un solo día, en envases estéri-les y fueron trasladados de manera inmediata hacia los laboratorios de la facultad de Ciencias Químicas de la UNACH- L.S.A. El análisis de la carga microbiana se realizó a través de un procedimiento in vitro en el laboratorio con cultivos sembrados en agar nutritivo de marca comercial Difco, se interpretaron los resultados, se verificó si estos valores están dentro de los parámetros internacionales para el uso y consumo humano, confirmando que existen valores mayores a las 200 UFC/ml. Finalmente, se procedió a la desinfección del agua, la que se realizó con hipoclorito de sodio al 5% y con digluconato de clorhexidina al 2%, cinco unidades detales para cada desinfectante respectivamente, se repitió el procedimiento de recolección de muestras así como también de análisis microbiológico post desinfección, se verificaron los resultados y se comparó la efectividad entre los desinfectantes empleados en la investigación, obtenido ausencia de UFC/ml para cada sustancia utilizada, dando el mante-nimiento al agua de los sistemas de irrigación.


A comparative observational study was carried out in the Dental Clinic of Universidad Nacional de Chimborazo (UNACH), during the academic period October 2015 - February 2016. The object of study was given by the existing microbial load in the irrigation system of 10 dental units; as well as the effect of 5% sodium hypochlorite and chlorhexidine diglucona-te on it. The samples were collected in two moments: after disinfection and after procedure performed. The intakes were made at the distal end of the irrigation systems of the triple syringe of the dental units, eliminating the amount of water that runs through the whole pipe and then obtaining the sample without interrupting the water flow in a sterile bottle. A total of 10 samples were collected, one from each dental unit, immediately closing the bottle and transferring them to the UNACH laboratory for processing. Before carrying out the disinfec-tion process, the laboratory results showed microbial contamination figures in the irrigation systems of the dental units above the acceptable values of CFU (Colony Forming Units). The use of 5% sodium hypochlorite and 2% chlorhexidine digluconate showed the effectiveness of these substances as antimicrobials in the disinfection of irrigation systems of dental units with similar levels of effectiveness in this regard.


Assuntos
Humanos , Hipoclorito de Sódio , Desinfecção , Desinfetantes de Equipamento Odontológico , Irrigação Terapêutica , Contaminação de Equipamentos , Clínicas Odontológicas , Anti-Infecciosos
11.
J Contemp Dent Pract ; 19(3): 296-300, 2018 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-29603702

RESUMO

AIM: This study aims to determine the role of mixing techniques of polyether impression materials and efficacy of disinfection on microbial colonization of these impression materials. MATERIALS AND METHODS: Polyether impression material was mixed using two methods: First by hand mixing (group I) and second using an automixer (group II) with a total of 100 samples. Four microbial strains were studied, which included Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans. After incubation, the bacterial colonies were counted, and then, disinfectant solution was applied. The effect of disinfection solution was evaluated for each specimen. RESULTS: The surface of polyether impression materials mixed with an automixer has less number of voids and overall a smoother surface as compared with the hand-mixed ones. On comparing the disinfection procedures, i.e., specimens without any disinfection and specimens after disinfection, statistically highly significant difference was seen between all the groups. CONCLUSION: We can conclude that impression mixing procedures are important in determining the surface characteristics of the impression and ultimately the colonization of bacteria and also determine the importance of disinfection on microbial colonization. CLINICAL SIGNIFICANCE: This study emphasises the deleterious role of nosocomial infections and specific measures that should be taken regarding the prevention of such diseases. Dental impressions are proved to be a source of such infections and may lead to transmission of such diseases. Thus, proper measures should be taken right from the first step of impression taking to minimizing and preventing such kind of contaminations in clinical practice.


Assuntos
Materiais para Moldagem Odontológica/uso terapêutico , Desinfecção/métodos , Carga Bacteriana , Candida albicans/metabolismo , Desinfetantes de Equipamento Odontológico/uso terapêutico , Técnica de Moldagem Odontológica/efeitos adversos , Escherichia coli/metabolismo , Humanos , Pseudomonas aeruginosa/metabolismo , Staphylococcus aureus/metabolismo
12.
Int J Prosthodont ; 31(2): 163-165, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29518811

RESUMO

PURPOSE: This study aimed to evaluate the possible effect of sealing materials on superstructure attachment (ie, tightening/loosening torque and implant-abutment gap) in two different implant systems. MATERIALS AND METHODS: A silicone, a chlorhexidine gel, and an industrial lubricant were tested. A 3D microscope was used for assessment of the implant-abutment gap, and the abutment screw was tightened and loosened with a digital torque screwdriver. A total of 20 implants per test group (10 BEGO Semados RI and 10 Nobel Biocare Replace Select Straight) were evaluated. RESULTS: The tested sealing materials did not influence the gap between implant and abutment, but the force necessary for loosening the abutment screws decreased significantly. CONCLUSION: Sealing materials may be useful against bacteria, but probably influence torque negatively.


Assuntos
Clorexidina/química , Dente Suporte , Desinfetantes de Equipamento Odontológico/química , Implantes Dentários , Lubrificantes/química , Silicones/química , Projeto do Implante Dentário-Pivô , Análise do Estresse Dentário , Contaminação de Equipamentos , Humanos , Teste de Materiais , Propriedades de Superfície , Torque
13.
J Water Health ; 16(1): 150-158, 2018 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-29424728

RESUMO

In this study we evaluated (1) the efficacy of a protocol that combines hydrogen peroxide (shock treatment) and ICX® tablets (continuous treatment) for the control of microbial contamination in dental unit water lines, and (2) the in vitro antimicrobial activity of ICX® tablets on collection and wild strains isolated from dental chair output waters. To assess the treatment effectiveness, the microbial load in the output water samples of three dental chairs were investigated: one control chair received only shock treatment. In vitro bactericidal activity was tested against Staphylococcus aureus and Pseudomonas aeruginosa. Data obtained from samples collected from chairs treated with ICX® and shock treatment and data from the control chair did not differ significantly on the basis of microbial load. In the in vitro study, the product was unable to kill Gram-negative bacteria. These results show that the continuous introduction of ICX® was not effective in maintaining low counts of the heterotrophic bacteria in the output water of dental devices, and shock treatment may be needed more frequently than monthly.


Assuntos
Biofilmes/efeitos dos fármacos , Desinfetantes de Equipamento Odontológico/farmacologia , Equipamentos Odontológicos/microbiologia , Contaminação de Equipamentos/prevenção & controle , Peróxido de Hidrogênio/farmacologia , Microbiologia da Água , Purificação da Água/métodos , Contagem de Colônia Microbiana , Humanos , Pseudomonas aeruginosa/efeitos dos fármacos , Staphylococcus aureus/efeitos dos fármacos
14.
J Endod ; 44(5): 806-812, 2018 May.
Artigo em Inglês | MEDLINE | ID: mdl-29426645

RESUMO

INTRODUCTION: The aim of this study was to test a new disinfection technology using biomimetic iron oxide nanoparticles (IO-NPs) with peroxidaselike activity to enhance antibacterial activity on root canal surfaces and in dentinal tubules. METHODS: The canal surfaces and dentinal tubules of single-rooted intact extracted teeth were infected by growing Enterococcus faecalis biofilms for 3 weeks. The samples were divided into 6 treatment groups: (1) phosphate-buffered saline (PBS) (negative control), (2) 3% hydrogen peroxide (H2O2) (test control), (3) IO-NPs (0.5 mg/mL) (test control), (4) IO-NPs (0.5 mg/mL) + 3% H2O2, (5) 3% sodium hypochlorite (positive control), and (6) 2% chlorhexidine (positive control). Environmental scanning electron microscopy coupled with energy-dispersive spectroscopy was used to confirm IO-NPs binding to the canal surface after a single treatment. Specimens were labeled with fluorescent staining for live/dead cells, and confocal laser scanning microscopy was used for the quantification of dead bacteria relative to the negative control (PBS). RESULTS: Both biofilm formation and dentinal tubule infection were successfully recapitulated using the in vitro model. IO-NPs were capable of binding to the infected canal surfaces despite a single, short-term (5-minute) treatment. IO-NP activation of H2O2 killed significantly more E. faecalis present on the canal surfaces and at different depths of dentinal tubules when compared with all other experimental groups (P < .05-.0005). CONCLUSIONS: The results reveal the potential to exploit nanocatalysts with enzymelike activity as a potent alternative approach for the treatment of endodontic infections.


Assuntos
Desinfetantes de Equipamento Odontológico/uso terapêutico , Nanopartículas Metálicas/uso terapêutico , Preparo de Canal Radicular/métodos , Biofilmes/efeitos dos fármacos , Biomimética , Clorexidina/uso terapêutico , Enterococcus faecalis/efeitos dos fármacos , Compostos Férricos/uso terapêutico , Humanos , Técnicas In Vitro , Microscopia Confocal , Irrigantes do Canal Radicular/uso terapêutico , Hipoclorito de Sódio/uso terapêutico
15.
J Contemp Dent Pract ; 19(1): 113-116, 2018 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-29358546

RESUMO

AIM: Species of Streptococcus, Escherichia coli, Staphylococcus, Actinomyces, Pseudomonas, Klebsiella, and Candida are commonly seen in the oral cavity. Impression materials are commonly contaminated with microorganisms. The present study was conducted to assess the disinfection efficacy of Epimax and 0.525% sodium hypochlorite on alginate impression over a period of 10 minutes. MATERIALS AND METHODS: This study was conducted in the Department of Prosthodontics in the year 2015. An alginate impression material was prepared. For each bacteria species, 15 samples were used. Out of 15 samples, 3 were used by 0.525% sodium hypochlorite for disinfection for 5 minutes and 3 others for 10 minutes. Similarly, 3 samples were used by Epimax for 5 minutes and other 3 for 10 minutes. Three samples were used as controls. Each sample was polluted with Candida albicans, Pseudomonas aeruginosa, and Staphylococcus aureus strains. RESULTS: There was no statistical difference in P. aeruginosa and C. albicans after 5 minutes, whereas S. aureus showed significant difference (p < 0.05). Epimax was found to be more effective in removing S. aureus as compared with other disinfectants. Both Epimax and 0.525% sodium hypochlorite did not show significant difference against P. aeruginosa and C. albicans, whereas significant difference was found between both agents against S. aureus (p < 0.05). It was seen that Epimax eliminated S. aureus after 5 minutes and P. aeruginosa after 10 minutes and 99.8% C. albicans after 10 minutes. About 0.525% sodium hypochlorite eliminated 99.1% of C. albicans after 10 minutes, whereas 98.5 and 99% of S. aureus and P. aeruginosa were eliminated after 10 minutes respectively. CONCLUSION: Both Epimax and 0.525% sodium hypochlorite can disinfect the alginate impression material against C. albicans, P. aeruginosa, and S. aureus strains. However, Epimax was found to be more effective against S. aureus as compared with 0.525% sodium hypochlorite. CLINICAL SIGNIFICANCE: Efficacy of disinfection of sodium hypo-chlorite and Epimax on alginate impression.


Assuntos
Alginatos/farmacologia , Candida albicans/efeitos dos fármacos , Desinfetantes de Equipamento Odontológico/farmacologia , Materiais para Moldagem Odontológica/farmacologia , Contaminação de Equipamentos/prevenção & controle , Pseudomonas aeruginosa/efeitos dos fármacos , Hipoclorito de Sódio/farmacologia , Staphylococcus aureus/efeitos dos fármacos , Ácido Glucurônico/farmacologia , Ácidos Hexurônicos/farmacologia
16.
Int Endod J ; 51(4): 457-468, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29023781

RESUMO

AIM: To compare the pre-sterilization cleaning of rotary Ni-Ti files of different sizes previously used a. ex vivo and b. clinically by a washer-disinfector, a regular ultrasonic bath, and the same ultrasonic bath in combination with a recently developed cavitation intensifying method. METHODOLOGY: Two sets of two hundred rotary Ni-Ti files, one previously used ex vivo and another one used clinically, were collected from the undergraduate and postgraduate clinics of the Academic Centre for Dentistry Amsterdam (ACTA). The instruments were immersed in an enzymatic solution and subsequently cleaned either by a washer-disinfector, a regular ultrasonic bath combined with a glass beaker, the same bath combined with a beaker lined with two cavitation intensifying sheets or with two standard plastic sheets. The positive control consisted of used files that did not undergo any cleaning and the negative control included new unused files. The instruments were then stained to reveal remaining protein material and scored under a stereoscopic microscope. The results were analysed by nonparametric statistical tests (α = 0.05). RESULTS: No significant difference was found between the combination of the ultrasonic bath and the regular glass beaker and the same ultrasonic bath with the beaker lined with the cavitation intensifying sheets. The washer-disinfector left significantly more debris compared to the latter group when clinically used files were evaluated (P ≤ 0.001). The effect of instrument size on cleaning was not consistent. CONCLUSION: None of the tested methods was able to remove all residual protein material from the files; however, it could be noted that this study did not follow the reprocessing protocol provided by the manufacturer.


Assuntos
Acústica/instrumentação , Desinfecção/métodos , Níquel , Preparo de Canal Radicular/instrumentação , Titânio , Ultrassom , Ligas Dentárias , Desinfetantes de Equipamento Odontológico , Instrumentos Odontológicos , Enzimas , Contaminação de Equipamentos/prevenção & controle , Reutilização de Equipamento , Humanos , Controle de Infecções Dentárias/instrumentação , Controle de Infecções Dentárias/métodos , Peptídeo Hidrolases , Proteínas , Esterilização , Propriedades de Superfície
17.
J Prosthodont ; 27(5): 461-468, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-27244668

RESUMO

PURPOSE: Dental prosthetic and orthodontic appliances are transported from the clinic to the laboratory for additions and repairs. These appliances, containing microbes from the oral flora, are a high risk for cross-contamination. The aim of this study was to evaluate the effect of chemical and ultrasound disinfection against two in vitro biofilms and an in vivo formed biofilm grown on unprepared and polished polymethyl methacrylate (PMMA) surfaces. MATERIALS AND METHODS: Rough and polished self-curing PMMA surfaces were infected with strains of both Candida albicans and Streptococcus oralis. After incubation, the samples were treated with different disinfection methods, including ultrasound treatment for both 15 and 30 seconds, and immersion in glutaraldehyde and alcohol-based chemical disinfectants (MD520 and Minuten, respectively). The disinfecting efficacy was assessed by colony forming units (CFU) analysis and by scanning electron microscopy (SEM). Furthermore the adequacy of bacterial elimination of application of 30-second ultrasound and MD520 was assessed on PMMA retrieved from ten volunteers by CFU analyses. ANOVA with p = 0.05 followed by the Tukey post hoc test and the Student t-test was used to analyze the data. RESULTS: The ultrasound treatment for 30 seconds, MD520, and Minuten were the most effective disinfectant methods as they reduced the microbial counts compared to the control (p < 0.05) as shown in the in vitro analyses. S. oralis adhered more to rough acrylic resin surfaces (p < 0.05). Ultrasound treatment was the most effective way to reduce microbial counts on PMMA exposed to oral flora (p = 0.043). CONCLUSION: Ultrasound treatment for 30 seconds was effective against C. albicans, S. oralis, and the oral flora as shown by testing microbial growth on agar plates and SEM.


Assuntos
Resinas Acrílicas/química , Biofilmes/efeitos dos fármacos , Desinfetantes de Equipamento Odontológico/farmacologia , Materiais Dentários/química , Desinfecção/métodos , Contaminação de Equipamentos/prevenção & controle , Glutaral/farmacologia , Polimetil Metacrilato/química , Ultrassom , Candida albicans , Contagem de Colônia Microbiana , Humanos , Microscopia Eletrônica de Varredura , Streptococcus oralis , Propriedades de Superfície
18.
J Prosthodont ; 27(5): 469-475, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-27385390

RESUMO

PURPOSE: To comparatively evaluate the antimicrobial efficacy of freshly prepared electrolyzed oxidizing water (EOW) with that of 2.4% glutaraldehyde (GA) and 1% sodium hypochlorite (SH) on clinically derived poly vinyl siloxane (PVS) impressions. MATERIALS AND METHODS: Four clinically derived PVS impressions each were obtained from 10 subjects. One impression from each subject was randomly kept as control (group I), without disinfection. The remaining three impressions were randomly disinfected by immersion in either 2.4% GA (group II), 1% SH (group III), or freshly prepared EOW (group IV). The samples were subjected to microbial culture individually on brain heart infusion (BHI) agar medium. The organisms isolated were confirmed by visual examination and gram staining. Mean colony forming units (CFU) were counted, log10 reduction obtained and compared. Data were statistically analyzed using one-way ANOVA, followed by multiple comparisons using Tukey-HSD post hoc tests (p < 0.05). RESULTS: Streptococci, Staphylococci, Pseudomonas, Candida, Proteus, Klebsiella, and E. coli were isolated from all impressions including the controls, except those disinfected by EOW. All three disinfectants showed significant reduction in CFU and log10 reduction values as compared to the controls. EOW showed significantly higher reduction in log10 values compared to GA and SH, whereas GA and SH showed similar reductions. EOW, GA, and SH showed kill rates of 100%, 99.60%, and 99.82%, respectively. CONCLUSION: EOW showed significant antimicrobial efficacy as evidenced by reduction in log10 values when used as an immersion disinfectant for PVS impressions when compared with the results obtained using GA and SH. These results need further investigation. CLINICAL IMPLICATIONS: EOW shows high antimicrobial efficacy when used as an immersion disinfectant as compared to GA and SH for clinically derived PVS impressions.


Assuntos
Antibacterianos/química , Anti-Infecciosos/química , Desinfetantes de Equipamento Odontológico/química , Materiais para Moldagem Odontológica/química , Desinfecção/métodos , Contaminação de Equipamentos/prevenção & controle , Glutaral/química , Polivinil/química , Siloxanas/química , Hipoclorito de Sódio/química , Adulto , Contagem de Colônia Microbiana , Técnica de Moldagem Odontológica , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Propriedades de Superfície
19.
Am J Dent ; 30(6): 350-352, 2017 12.
Artigo em Inglês | MEDLINE | ID: mdl-29251459

RESUMO

PURPOSE: This is a review of the literature on nonsurgical treatment of non-healing medication related osteonecrosis of the jaw (MRONJ) utilizing a phosphate buffer-stabilized 0.1% chlorine dioxide mouthrinse. METHODS: A literature search in PubMed revealed only six case reports. MRONJ lesion site description, patient's medication history, the healing time, and the MRONJ treatment protocol followed by those authors were recorded. Additional literature review of the scientific mechanism, risks and benefits, safety and efficacy of the phosphate buffer-stabilized 0.1% chlorine dioxide mouthrinse was also performed and discussed. RESULTS: Many of the authors of the published case reports utilized 0.12% chlorhexidine as the initial mouthrinse, but the lesions did not decrease in size. After switching to a phosphate buffer-stabilized 0.1% chlorine dioxide mouthrinse for a duration ranging from 1-12 months, there was complete healing of the MRONJ lesions in all of the cases. The phosphate buffer-stabilized 0.1% chlorine dioxide mouthrinse can be helpful in the management of active MRONJ lesions as well as the prevention of recurrent MRONJ lesions in the susceptible patient population. CLINICAL SIGNIFICANCE: This literature review supports the use of phosphate buffer-stabilized 0.1% chlorine dioxide mouthrinse in the management of MRONJ lesions either as a first line of therapy or after 0.12% chlorhexidine had not been effective.


Assuntos
Osteonecrose da Arcada Osseodentária Associada a Difosfonatos/tratamento farmacológico , Compostos Clorados/uso terapêutico , Desinfetantes de Equipamento Odontológico/uso terapêutico , Óxidos/uso terapêutico , Humanos , Osteonecrose , Fosfatos
20.
J Contemp Dent Pract ; 18(12): 1185-1189, 2017 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-29208796

RESUMO

AIM: The aim of this study was to evaluate the tensile strength of die stone incorporated with sodium and calcium hypochlorite as disinfectants. MATERIALS AND METHODS: Two commercially available type IV die stone (Kalrock: Kalabhai Karson Pvt., Ltd and Pearlstone: Asian Chemicals) and two commercially available disinfectant solutions (sodium hypochlorite and calcium hypochlorite: Beachem Laboratory Chemical Private Limited, Chennai and Leo Chem Private Limited, Bengaluru) were used in this study, and the tensile strength was measured using Lloyd's Universal Testing Machine. RESULTS: The results show that incorporating the disinfecting solutions decreases the tensile strength of both products. The effect of decreasing tensile strength on type IV gypsum product is seen more in calcium hypochlorite when compared with sodium hypochlorite disinfecting solution, and the tensile strength of Kalrock specimens is higher than Pearlstone specimens after disinfecting with sodium hypochlorite and calcium hypochlorite solution. The statistical results also show significant results in all the groups when compared with the control group. CONCLUSION: The incorporation of sodium and calcium hypochlorite disinfecting solutions is not an encouraging method for both die materials as it reduces the tensile strength of type IV gypsum product. Tensile strength of Kalstone® die material is superior than Pearlstone® die material after mixing with sodium hypochlorite and calcium hypochlorite. CLINICAL SIGNIFICANCE: According to the recommendations of Americans with Disability Act (ADA) and the Centers for Disease Control and Prevention, disinfecting the whole cast without or minimal changes in physical and mechanical properties was the motto of the study. The tensile strength in type IV gypsum product plays a most important role in retrieval of cast from impression, especially in narrow tooth preparation. This study reveals that incorporating method of disinfecting solutions is not recommended as it reduces the tensile strength.


Assuntos
Compostos de Cálcio , Desinfetantes de Equipamento Odontológico , Materiais para Moldagem Odontológica , Hipoclorito de Sódio , Resistência à Tração , Humanos , Técnicas In Vitro , Transmissão de Doença Infecciosa do Profissional para o Paciente/prevenção & controle
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