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1.
Talanta ; 206: 120236, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31514837

RESUMO

This work presents a reliable analytical procedure combining micro-extraction by packed sorbent (MEPS) and ultra-high performance liquid chromatography-electrospray ionization tandem mass spectrometry to determine 8-iso prostaglandin F2α, 8-iso prostaglandin E2 and prostaglandin E2 in dried blood spots (DBSs). To reach this goal, we optimized a fast semi-automated MEPS procedure for the clean-up and pre-concentration of the analytes extracted from a single DBS (50 µL) by a 70:30 v/v methanol:water mixture. Limits of detection of about 20 pg mL-1, satisfactory recoveries (90-110%) and very good intra- and inter-day precisions (RSD ≤10%) were obtained for all the analytes. The innovative addition of internal standards on the filter paper before DBS sampling allowed to compensate changes in the amount of analyte during storage. Since prostanoids and isoprostanoids are biomarkers involved in the pathogenesis and progression of many diseases (e.g. ductal patency, diabetic nephropathy, and acute lung injury), our analytical method offers interesting diagnostic and prognostic opportunities in the medical field. The present method is currently used for the analysis of such biomarkers in DBSs from preterm newborns collected in the clinical setting.


Assuntos
Dinoprosta/análogos & derivados , Dinoprostona/análogos & derivados , Dinoprostona/sangue , Teste em Amostras de Sangue Seco/métodos , Isoprostanos/sangue , Biomarcadores/sangue , Cromatografia Líquida de Alta Pressão/métodos , Dinoprosta/sangue , Humanos , Recém-Nascido , Limite de Detecção , Microextração em Fase Sólida/métodos , Espectrometria de Massas por Ionização por Electrospray/métodos , Espectrometria de Massas em Tandem/métodos
2.
Theriogenology ; 141: 98-104, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31536862

RESUMO

The objective was to evaluate the association between postpartum health disorders, reproductive responses and pregnancy status in lactating multiparous cyclic Holstein cows. Cows were retrospectively categorized as healthy (n = 70) or sick (n = 60) based on postpartum health records and serum metabolites. Sick cows were further categorized as having metabolic (MET; n = 35), infectious (INF; n = 15), or both diseases (MET/INF; n = 10). Blood samples were collected on d 7 and 14 after calving to determine serum concentrations non-esterified fatty acids (NEFA), ß-hydroxybutyrate (BHB) and aspartate aminotransferase (AST), on d 0 (TAI), 8, 16, 18 and 20 after TAI to determine concentrations of progesterone (P4; d 0, 8, 16, 18 and 20) and prostaglandin F metabolite (PGFM; d 16, 18 and 20) and interferon-stimulated gene-15 (ISG15; d 16) relative mRNA expression. Cyclicity was determined by transrectal ultrasonography 30 d postpartum and cows were subjected to a GnRH-based TAI protocol (to classify cows bearing a visible CL as cyclic). Prediction of pregnancy status on d 16 after TAI was determined by ISG15 mRNA gene expression relative to ß actin and following, pregnancy was diagnosed by transrectal ultrasonography at 32 and 60 d after TAI. An interaction (P = 0.04) between occurrence of disease and pregnancy status was detected for the expression of ISG15 in blood, with healthy pregnant healthy cows having the greatest relative expression of ISG15. Postpartum health disorders were associated with reduced concentration (P < 0.05) of serum P4 post TAI. However, serum P4 concentrations at TAI were greater (P = 0.01) in sick cows (0.65 ±â€¯0.09, 0.86 ±â€¯0.13 and 0.75 ±â€¯0.10 ng/mL for MET, INF and MET/INF cows, respectively) compared with that in healthy cows (0.24 ±â€¯0.10 ng/mL). Serum concentrations of PGFM after TAI was reduced in healthy cows, regardless of pregnancy status. Pregnancy status on d 16 after TAI predicted by ISG15 mRNA expression and P/AI on d 32 and 60 after TAI based on ultrasonography, were negatively affected (P < 0.05) by occurrence of health disorders. Similarly, pregnancy loss from d 16 to 32 and d 16 to 60 after TAI was greater (P < 0.05) in sick cows compared to that in healthy cows. However, neither P/AI nor pregnancy loss were associated to the category of postpartum health disorder. Cows affected by postpartum health disorders had overall reduced P4 and greater PGFM serum concentrations after TAI, which were associated with reduced pregnancy success and enhanced pregnancy loss. Collectively, our findings support the hypothesis of a carryover effect of disease on reproductive responses, embryo survival and maintenance of pregnancy in lactating dairy cows independent of the category of postpartum health disorder.


Assuntos
Doenças dos Bovinos , Inseminação Artificial/veterinária , Animais , Composição Corporal , Bovinos , Dinoprosta/análogos & derivados , Dinoprosta/sangue , Feminino , Lactação , Período Pós-Parto , Gravidez , Progesterona/sangue , Reprodução/fisiologia , Estudos Retrospectivos
3.
Endocrinol. diabetes nutr. (Ed. impr.) ; 66(9): 540-549, nov. 2019. tab, graf
Artigo em Inglês | IBECS | ID: ibc-184376

RESUMO

Introduction: Few studies assessing the relationship between oxidative stress and glycemic variability in children with type 1 diabetes mellitus (T1DM) are available, and most of them reported no significant results. Objective: To assess the relationship between glucose control, glycemic variability, and oxidative stress as measured by urinary excretion of 8-iso-prostanglandin F2-alpha (8-iso-PGF2alfa) in children with T1DM. Materials and methods: A cross-sectional study including 25 children with T1DM. Participants were evaluated during five days in two different situations: 1st phase during a summer camp, and 2nd phase in their everyday life at home. The following data were collected in each study phase:. Six capillary blood glucose measurements per day. Mean blood glucose (MBG) levels and glucose variability parameters, including standard deviation, coefficient of variation, and mean amplitude of glycemic excursions (MAGE), were calculated. - Capillary HbA1c level. - 24-h urine sample to measure 8-iso-PGF2alfa. Results: There were no statistically significant differences in urinary 8-iso-PGF2alfa levels (142 ± 37 vs. 172 ± 61 pg/mg creatinine) and glucose control and glycemic variability parameters between both phases. In the 2nd phase, statistically significant correlations were found between urinary 8-iso-PGF2alfa and HbA1c levels (r = 0.53), MBG (r = 0.72), standard deviation (r = 0.49), and MAGE (r = 0.42). No significant correlations between glucose control, glycemic variability and urinary 8-iso-PGF2alfa excretion were found in the 1st phase. Conclusions: A significant correlation was found between glycemic variability and HbA1c level and urinary 8-iso-PGF2α excretion in a group of children with T1DM during their daily lives. Additional studies are needed to confirm this finding and to explore its long-term impact on health


Introducción: En niños con diabetes tipo 1 (DM1) hay pocos estudios que evalúen la relación entre estrés oxidativo y variabilidad glucémica, y la mayoría de ellos no encuentran resultados significativos. Objetivo: Evaluar la relación entre control metabólico, variabilidad glucémica y estrés oxidativo medido por la excreción urinaria de 8-iso-prostaglandina F2 alfa (8-iso-PGF2alfa) en niños con DM1. Material y método: Estudio transversal que incluyó 25 niños con DM1. Los participantes fueron evaluados durante 5 días en 2 situaciones diferentes: 1.a fase durante un campamento de verano y 2.a fase durante su actividad habitual en domicilio. En cada fase se recogieron:- Seis determinaciones de glucemia capilar diarias. Se calcularon glucemia media y parámetros de variabilidad glucémica: desviación estándar, coeficiente de variación y «mean amplitude of glycemic excursions» (MAGE). - HbA1c capilar. - Muestra de orina de 24h para la determinación de 8-iso-PGF2alfa. Resultados: No se encontraron diferencias estadísticamente significativas en excreción urinaria de 8-iso-PGF2alfa (142 ± 37 vs. 172 ± 61 pg/mg creatinina) y parámetros de control y variabilidad glucémicos entre las fases. En la 2.a fase se observaron correlaciones estadísticamente significativas entre 8-iso-PGF2alfa urinario con HbA1c (r = 0,53), glucemia media (r = 0,72), desviación estándar (r = 0,49) y MAGE (r = 0,42). En la 1.a fase del estudio no se han detectado correlaciones significativas. Conclusiones: Se ha encontrado una correlación significativa entre parámetros de variabilidad glucémica y HbA1c con la excreción urinaria de 8-iso-PGF2alfa en un grupo de niños con DM1 evaluados durante su vida diaria. Son necesarios más estudios para confirmar estos resultados y evaluar el impacto a largo plazo sobre la salud


Assuntos
Humanos , Criança , Diabetes Mellitus Tipo 1/complicações , Estresse Oxidativo , Índice Glicêmico , Metabolismo Basal , Dinoprosta/análogos & derivados , Diabetes Mellitus Tipo 1/sangue , Diabetes Mellitus Tipo 1/diagnóstico , Estudos Transversais , Diabetes Mellitus Tipo 1/urina , Dinoprosta/sangue
4.
J Int Soc Sports Nutr ; 16(1): 31, 2019 Jul 31.
Artigo em Inglês | MEDLINE | ID: mdl-31366352

RESUMO

BACKGROUND: Cashew apple juice (CAJ) was shown to improve immunological mechanisms by regulating a balance between reactive oxygen species and antioxidant concentrations. However, no study exploring the effects of the CAJ and training status on the immune system and oxidative stress induced by exercise. Therefore, we investigated the effects of CAJ supplementation primarily on leukocyte counts and secondary on oxidative stress and cortisol changes after high-intensity exercise in trained and untrained men. METHODS: Ten moderately (endurance) trained (Age = 21.5 ± 0.97 yr., VO2max = 45.6 ± 4.12 mL/kgBM/min) and ten sedentary men (Age = 20.4 ± 2.72 yr., VO2peak = 32.2 ± 7.26 mL/kgBM/min) were randomized to ingest either daily CAJ or a placebo at 3.5 mL/kgBM/day for 4 weeks, with a four-week washout period. Before and after each period, they performed 20-min, high-intensity cycling (85% VO2max), with blood samples collected immediately preceding and the following exercise. Samples were analyzed to determine leukocyte counts, malondialdehyde, 8-isoprostane, and cortisol concentrations. A repeated measures analysis of variance was used to examine the effects of supplement and training status over time with an alpha level of 0.05. RESULTS: There was no interaction between supplement and training status on those variables before and after exercise. However, CAJ raised resting neutrophil counts and exercise-induced leukocyte counts in the trained group (all p < 0.05). Besides, CAJ significantly reduced plasma malondialdehyde concentrations at rest and after exercise and reduced the post-exercise plasma 8-isoprostane concentration in both groups of subjects (p < 0.05). Moreover, CAJ reduced plasma cortisol after exercise in the untrained subjects. CONCLUSIONS: We suggest that 4-week CAJ supplementation can enhance exercise-induced leukocyte and resting neutrophil counts in trained men. The possible mechanism is a reduction in oxidative stress. However, the supplementation did not change the immune responses of untrained men, but it did reduce stress hormone concentrations. TRIAL REGISTRATION NUMBER: TCTR20181127002 Registered 26 November 2018 "retrospectively registered".


Assuntos
Suplementos Nutricionais , Exercício , Sucos de Frutas e Vegetais , Contagem de Leucócitos , Estresse Oxidativo , Anacardium , Estudos Cross-Over , Dinoprosta/análogos & derivados , Dinoprosta/sangue , Método Duplo-Cego , Humanos , Hidrocortisona/sangue , Masculino , Malondialdeído/sangue , Malus , Neutrófilos/citologia , Espécies Reativas de Oxigênio/metabolismo , Fenômenos Fisiológicos da Nutrição Esportiva , Adulto Jovem
5.
Theriogenology ; 135: 38-45, 2019 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-31200095

RESUMO

This study aimed to examine the etiology of canine dystocia by measuring the relative expression of oxytocin receptor (OXTR) mRNA and the concentration of serum progesterone, plasma PGF2α metabolite (PGFM), and blood ionized calcium (iCa) near term and in dystocia. Altogether 58 bitches were included in this study, 41 of which underwent cesarean section (CS). The four CS groups were based on history: complete uterine inertia (CUI; n = 7), partial uterine inertia (PUI; n = 13), obstructive dystocia (OD; n = 10), and elective cesarean section (ECS; n = 11). An additional group of medically treated dystocia without CS (MD; n = 8) and a control group (C; n = 9) with normal parturition (without CS and medical treatment) were also formed. Blood samples were taken prior to CS or medical treatment. Progesterone concentrations were highest in the ECS and a significant difference (p < 0.05) was observed between the ECS and the OD and between the ECS and the combined dystocia (CUI, PUI, OD, MD) groups (COMB). Highest concentrations of PGFM was observed in the C, the difference being significant (p < 0.05) between the C and the ECS and between the C and the COMB group. The progesterone:PGFM ratio was significantly (p < 0.05) higher in the ECS than in the C and the COMB group. No significant difference (p > 0.05) was observed in iCa concentrations between the groups. Relative OXTR mRNA expression was evaluated with real-time PCR from full-thickness uterine samples taken from the incision site during CS. The expression was highest in the ECS and the difference in expression was significant (p < 0.05) between the ECS and the OD and between ECS and the combined dystocia (CUI, PUI, OD) groups (COMB2). The study supports previous reports of decreasing progesterone and increasing PGFM during prepartum luteolysis. Upregulation of OXTR occurs near term. In obstructive dystocia, a prolonged influence of oxytocin and uterine exhaustion may lead to downregulation of OXTR. Complete primary uterine inertia may have a different etiology as no clear decrease in OXTR was observed in CUI as in OD. It remains unclear if parturition ceases because of uterine inertia or if uterine inertia occurs because of ceased parturition and desensitization of receptors.


Assuntos
Cálcio/sangue , Dinoprosta/análogos & derivados , Distocia/veterinária , Progesterona/sangue , Receptores de Ocitocina/metabolismo , Animais , Dinoprosta/sangue , Doenças do Cão/sangue , Doenças do Cão/metabolismo , Cães , Distocia/metabolismo , Feminino , Gravidez , Receptores de Ocitocina/genética
6.
Int J Food Sci Nutr ; 70(7): 887-893, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31148492

RESUMO

To investigate changes in resting metabolic rate and 8-isoprostane, an oxidative stress biomarker, following acute dietary nitrate supplementation in healthy males and females. In a randomised, double-blind, cross-over study, 10 males and seven females (age range 19-25 years) underwent protocol familiarisation (visit 1), baseline assessments (visits 2 and 4) and assessments following supplementation, placebo or 6.2 mmol nitrate, 2 hours prior to visits 3 and 5. Participants completed a 30-minute RMR test with visits 2 and 3 on consecutive days, separated by a week-long washout period concluding with visits 4 and 5 on consecutive days. Plasma nitrate/nitrite (NOx) significantly increased (p ≤ 0.05) following dietary nitrate consumption compared to baseline values. No significant effect on resting metabolism (p = 0.194) or 8-isoprostane (p = 0.660) was observed following dietary nitrate supplementation. Dietary nitrate increases NO bioavailability, but acute supplementation does not effect resting metabolism or 8-isoprostane in healthy males and females.


Assuntos
Metabolismo Basal/efeitos dos fármacos , Biomarcadores/metabolismo , Suplementos Nutricionais , Dinoprosta/análogos & derivados , Nitratos/farmacologia , Estresse Oxidativo/efeitos dos fármacos , Adulto , Estudos Cross-Over , Dinoprosta/sangue , Método Duplo-Cego , Feminino , Humanos , Masculino , Nitratos/sangue , Nitritos/sangue , Adulto Jovem
7.
J Environ Sci Health B ; 54(8): 623-639, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31033394

RESUMO

Background: There is need to understand biological markers and mechanisms in Gulf War illness (GWI). Goal: To examine whether and how eicosanoids - prostaglandins and leukotrienes - are altered in veterans with GWI. Methods: Seventy participants including 37 GWI and 33 healthy controls, shared exposure information, and had plasma eicosanoids assessed - prostaglandin F2 alpha (pgf2α), prostaglandin D2 (pgd2), leukotriene B4 (lb4) among others. Values were compared for GWI versus controls. Eicosanoid intercorrelations were compared in cases vs. controls. For the most significantly altered eicosanoid in GWI, exposure and symptom relations were assessed. Results: Prostaglandins and leukotrienes were depressed in GWI, strongest for pgf2α, then lb4. Eicosanoid intercorrelations differed in GWI vs. controls. Fuel-solvent, pesticide, radioactive chemicals and metal exposures related negatively to pgf2α; as, in GWI, did chemical attack and vaccines. Multivariate predictors included fuels-solvents and radioactive chemicals (negative); tetanus vaccine and herbicides (positive). Fuels-solvents and radioactive chemicals predicted lower pgf2α in cases, controls, and all participants controlled for case status. Lower pgf2α related to GWI "Kansas criteria" domains of pain, respiratory, and (borderline significantly) skin symptoms. Conclusion: Multiple eicosanoids are depressed in GWI, particularly pgf2α and lb4. Prior fuel-solvent exposures, radioactive chemicals, and (in GWI cases) vaccines were linked to lower pgf2α.


Assuntos
Leucotrienos/sangue , Síndrome do Golfo Pérsico/sangue , Prostaglandinas/sangue , Biomarcadores/sangue , Estudos de Casos e Controles , Dinoprosta/sangue , Feminino , Guerra do Golfo , Humanos , Masculino , Pessoa de Meia-Idade , Síndrome do Golfo Pérsico/etiologia , Saúde dos Veteranos
8.
Artigo em Inglês | MEDLINE | ID: mdl-30928411

RESUMO

The objective of the present study was to determine the influence of dietary supplementation with pomegranate seed oil (PSO) and/or an aqueous extract of dried bitter melon fruits (BME) on breast cancer risk and fatty acid profile in serum of female rats with chemical carcinogen-inflicted mammary tumours. Sprague-Dawley rats (n = 96) were fed control diet or experimental diets supplemented with 0.15 ml PSO/day, BME or jointly PSO and BME. After 21 weeks mammary tumours were subjected to histopathological examination and in serum fatty acids, 8-isoprostaglandin F2α content and indices of desaturases activity were analysed. Supplementation of the diet with PSO and BME did not inhibit the breast cancer formation. Conjugated linolenic acids (CLnA), present in PSO, were converted into cis-9, trans-11 conjugated linoleic acid (CLA), however, its content was lower in groups treated with a carcinogen. A similar tendency was observed for the content of SFA, MUFA, PUFA, 8-iso PGF2α and the activity of Δ6-desaturase. Enhanced pro-carcinogenic effect of 7,12-dimethylbenz[a]anthracene (DMBA), caused by applied supplements, may be a result of their influence on DMBA metabolism.


Assuntos
Suplementos Nutricionais , Ácidos Graxos/sangue , Neoplasias Mamárias Experimentais/patologia , Momordica charantia/química , Óleos Vegetais/farmacologia , Sementes/química , Animais , Peso Corporal/efeitos dos fármacos , Carcinogênese/efeitos dos fármacos , Dinoprosta/análogos & derivados , Dinoprosta/sangue , Relação Dose-Resposta a Droga , Feminino , Neoplasias Mamárias Experimentais/sangue , Neoplasias Mamárias Experimentais/metabolismo , Neoplasias Mamárias Experimentais/prevenção & controle , Tamanho do Órgão/efeitos dos fármacos , Ratos , Ratos Sprague-Dawley , Risco
9.
Artigo em Inglês | MEDLINE | ID: mdl-30731263

RESUMO

This paper reports an online SPE-LC-MS/MS method for the simultaneous quantification of prostaglandins (PGE2 and PGF2α) in menstrual fluid samples. To meet this goal human peripheral serum was used as surrogate matrix. The analytes were trapped on an OASIS HLB cartridge for 3 min, for sample cleanup and enrichment, and then transferred during only 42 s to an HSS T3 C18 analytical column, for separation and analysis. Prostaglandins (PGs) were detected by selected reaction monitoring in negative ion mode, PGE2 (m/z 351 → 315) and PGF2α (m/z 353 → 193) using isotope-labeled internal standard (PGE2-d4, m/z 355 → 319). The concentration linear range was of 10.34-1.034 ng mL-1 and the lower limit of quantification (LLOQ) was 10.34 ng mL-1 for both PGs. Validation parameters were successfully assessed according to the European Medicines Agency guideline (EMA), also comprising the FDA normative. The method showed no matrix effect and process efficiency around 100%, in addition to only 15 min of analysis time with lower solvent consumption. The method application was carried out using two menstrual fluid sample groups: control (n = 15) and treatment group (n = 7; samples from women that used Tahiti lemon juice). The PGF2α levels were found to be higher in treated group than in control group (p ≤ 0.05), denoting an effect of the intake of Tahiti lemon juice on the menstrual inflammatory process. The on-line method herein reported could be useful for the analysis of PGs from large research studies.


Assuntos
Cromatografia Líquida/métodos , Dinoprosta/sangue , Dinoprostona/sangue , Menstruação/sangue , Extração em Fase Sólida/métodos , Espectrometria de Massas em Tandem/métodos , Adolescente , Adulto , Dinoprosta/isolamento & purificação , Dinoprostona/isolamento & purificação , Feminino , Humanos , Limite de Detecção , Modelos Lineares , Reprodutibilidade dos Testes , Adulto Jovem
10.
J Endocrinol Invest ; 42(1): 75-83, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-29687417

RESUMO

PURPOSE: To evaluate the plasma level of 8-isoprostanes in women with polycystic ovary syndrome. To also investigate whether there is a relationship between 8-isoprostanes and several cardiovascular risk factors. METHODS: A total of 125 women with polycystic ovary syndrome and 169 healthy women were enrolled in this case-control study. 8-Isoprostanes and different parameters were measured in all subjects. Patients were evaluated for the presence of polycystic ovary syndrome according to the Rotterdam Consensus Conference criteria. RESULTS: 8-Isoprostanes levels were significantly higher in patients with polycystic ovary syndrome (138.4 ± 104.1 pg/mL) compared with control group (68.6 ± 34.3 pg/mL) (p < 0.001). The mean of triglycerides, lipid accumulation product, C-reactive protein, homocysteine, insulin, and homeostatic model assessment for insulin resistance were significantly higher in polycystic ovary syndrome patients with high 8-isoprostanes than those with normal 8-isoprostanes (p < 0.05). The Pearson correlation analyses showed that 8-isoprostanes levels in polycystic ovary syndrome group had a positive correlation with waist circumference, triglycerides, low-density lipoprotein cholesterol, apolipoprotein B, homocysteine, insulin, homeostatic model assessment for insulin resistance. CONCLUSIONS: Patients with polycystic ovary syndrome have higher 8-isoprostanes levels and it is associated with several cardiovascular risk factors.


Assuntos
Doenças Cardiovasculares/sangue , Doenças Cardiovasculares/epidemiologia , Dinoprosta/análogos & derivados , Síndrome do Ovário Policístico/sangue , Síndrome do Ovário Policístico/epidemiologia , Adulto , Biomarcadores/sangue , Doenças Cardiovasculares/diagnóstico , Estudos de Casos e Controles , Dinoprosta/sangue , Feminino , Humanos , Isoprostanos/sangue , Obesidade/sangue , Obesidade/diagnóstico , Obesidade/epidemiologia , Síndrome do Ovário Policístico/diagnóstico , Fatores de Risco , Adulto Jovem
11.
J Gerontol A Biol Sci Med Sci ; 74(1): 121-128, 2019 01 01.
Artigo em Inglês | MEDLINE | ID: mdl-30371905

RESUMO

Background: A strong association of serum 25-hydroxyvitamin-D levels (25(OH)D) with all-cause mortality has been shown previously and 25(OH)D could be a useful aging marker. Methods: The analysis was performed in a population-based, cohort study from Germany with 9,940 participants, aged 50-74 years at baseline. A general linear model was used to assess associations of 25(OH)D levels with chronological age and the aging markers leukocyte telomere length (LTL), epigenetic age acceleration, and 8-isoprostane levels. A multivariate Cox regression model was applied to explore the independent and combined associations of these biomarkers with all-cause mortality (2,204 deaths occurred during a median follow-up of 14.3 years). Results: On average, study participants lost 2.9 nmol/L 25(OH)D each 10 years of age. Increasing 25(OH)D levels were significantly associated with decreasing levels of 8-isoprostane levels but neither with LTL nor epigenetic age acceleration. The association of 25(OH)D quartiles with mortality was almost unchanged after adjusting for all aging markers (1.6-fold increased mortality in bottom quartile compared with top quartile). All aging markers were independent mortality predictors and subjects with unfavorable values for 4, 3, 2, and 1 aging marker(s) had 4.3-, 2.9-, 2.2, and 1.4-fold increased mortality, respectively. Conclusions: The 25(OH)D level can be regarded as an aging marker because it is linearly associated with age and an independent mortality predictor. Mechanisms linking vitamin D to healthy aging are unique and can neither be fully explained by aging of the epigenome, loss of telomeres, or antioxidative effects of vitamin D metabolites.


Assuntos
Envelhecimento/sangue , Dinoprosta/análogos & derivados , Epigênese Genética/genética , Epigenômica/métodos , Telômero/metabolismo , Deficiência de Vitamina D/sangue , Vitamina D/análogos & derivados , Idoso , Biomarcadores/sangue , Causas de Morte/tendências , Dinoprosta/sangue , Feminino , Seguimentos , Alemanha/epidemiologia , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Vitamina D/sangue , Deficiência de Vitamina D/genética , Deficiência de Vitamina D/mortalidade
12.
Rom J Intern Med ; 56(4): 257-264, 2018 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-30521476

RESUMO

INTRODUCTION: Obesity is becoming one of the leading risk factors of coronary heart disease, hypertension, cerebrovascular disease. Despite the presence of a large number of antihypertensive agents and scientific substantiation of antihypertensive treatment principles it would be wrong to assume that the problem is completely solved. Development of endothelial dysfunction is one of the key pathogenic mechanisms in hypertension. This process is proven to have contributed by immune inflammation activation which is mediated by pro-inflammatory cytokines and oxidative stress. AIMS: To investigate the additional benefits of the combined antihypertensive therapy with lacidipine and candesartan on the basis of studying their antioxidant properties, impact on endothelial function and pro-inflammatory cytokines activity in hypertensive patients with overweight and obesity. METHODS: A combination of a calcium channel blocker and angiotensin receptor blocker (lacidipine 2 mg, 4 mg, and candesartan 4mg, 8mg, 16mg) was prescribed to 30 patients with essential hypertension of grades 1-3, 30 to 65 years old (mean age - 54.7 ± 5.8 years), who previously have not been receiving regular antihypertensive therapy. RESULTS: During the course of combined antihypertensive therapy with lacidipine and candesartan, a significant reduction in i-NOS activity, TNF-α to its type I soluble receptor ratio (TNF- α/sTNF-αRI), and oxidative stress marker - 8-iso-PgF2α has been observed. Activity of e-NOS, levels of SOD and catalase, in contrast, have increased by the end of observation period. CONCLUSION: The improvement of endothelial function due to lower level of oxidative stress and a significant decrease of immune activation has been observed in hypertensive patients with overweight and obesity under the influence of combined antihypertensive therapy with lacidipine and candesartan.


Assuntos
Anti-Hipertensivos/uso terapêutico , Benzimidazóis/uso terapêutico , Di-Hidropiridinas/uso terapêutico , Hipertensão/tratamento farmacológico , Obesidade/complicações , Tetrazóis/uso terapêutico , Adulto , Idoso , Anti-Hipertensivos/farmacologia , Benzimidazóis/farmacologia , Estudos de Casos e Controles , Di-Hidropiridinas/farmacologia , Dinoprosta/análogos & derivados , Dinoprosta/sangue , Quimioterapia Combinada , Endotélio Vascular/efeitos dos fármacos , Feminino , Humanos , Hipertensão/sangue , Hipertensão/complicações , Masculino , Pessoa de Meia-Idade , Obesidade/sangue , Tetrazóis/farmacologia , Fator de Necrose Tumoral alfa/sangue
13.
BMC Vet Res ; 14(1): 334, 2018 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-30404636

RESUMO

BACKGROUND: Postpartum dysgalactia syndrome (PDS) in sows is difficult to diagnose and the pathogenesis is obscure. Hormonal changes related to the disease are often difficult to distinguish from those found in the normal transition period from gestation to lactation. The study aimed to investigate metabolic and hormonal changes related to PDS with the goal of identifying potential biomarkers in sows suffering from PDS (PDS+). Selected biomarkers were examined by comparing 38 PDS+ sows with 38 PDS negative (PDS-) sows. The sows were sampled every 24 h from 60 h ante partum (a.p.) to 36 h post partum (p.p.). RESULTS: Compared to the baseline (60 to 36 h a.p.), cortisol in serum and saliva and fasting blood glucose concentrations increased in PDS+ as well as PDS- sows. C-peptide decreased relative to the baseline in PDS+ sows, and prolactin and 8-epi prostaglandin F2 alpha (8-epi-PGF2α) decreased in PDS- sows. Concentrations of cortisol in serum and saliva, salivary chromogranin A (CgA), fasting blood glucose, C-peptide, and 8-epi-PGF2α differed significantly between PDS+ and PDS- sows, with levels of cortisol in serum and saliva, salivary CgA, and 8-epi-PGF2α in serum being different in the two groups already before parturition. Concentrations of salivary CgA were significantly lower in PDS- sows than in PDS+ sows during the entire study period. CONCLUSIONS: The results suggest that salivary CgA, cortisol and serum 8-epi-PGF2α may potentially serve as early diagnostic indicators for PDS. The consistently higher salivary CgA concentration in PDS+ sows compared to PDS- sows may indicate that homeostatic disturbances are present between 36 to 60 h before parturition in sows developing PDS. The higher serum and saliva cortisol concentration in PDS+ sows compared to PDS- sows could reflect an early sign of inflammation or stress. The significantly lower C-peptide in PDS+ sows compared to PDS- sows may reflect a lower food intake. Our results contribute to the understanding of the pathogenesis of PDS, and the homeostatic disturbances detected before parturition warrants further investigation. The diagnostic potential of the markers identified in this study should be investigated further in a larger population of sows.


Assuntos
Transtornos da Lactação/veterinária , Doenças dos Suínos/fisiopatologia , Animais , Glicemia/análise , Peptídeo C/sangue , Estudos de Casos e Controles , Cromogranina A/sangue , Dinoprosta/análogos & derivados , Dinoprosta/sangue , Feminino , Hidrocortisona/análise , Hidrocortisona/sangue , Transtornos da Lactação/metabolismo , Transtornos da Lactação/fisiopatologia , Parto/metabolismo , Parto/fisiologia , Período Pós-Parto/metabolismo , Período Pós-Parto/fisiologia , Prolactina/sangue , Saliva/química , Suínos , Doenças dos Suínos/metabolismo
14.
Bull Exp Biol Med ; 165(6): 781-785, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30353332

RESUMO

We studied the expression of Hif-1α, Nf-κb, and Vegf genes in the liver and serum levels of HIF-1α, erythropoietin, VEGF, TGF-ß, 8-isoprostane, and corticosterone in Wistar rats with different resistance to hypoxia in 5 and 90 min after acute exposure to hypobaric hypoxia. In 5 min after hypoxic exposure, Hif-1α expression in the liver and serum levels of erythropoietin, VEGF, and TGF-ß in high-resistant rats were higher than in low-resistant animals. In highresistant rats, the increment in expression of Nf-κb gene responsible for the control over the inflammatory processes was more pronounced than in low-resistant animals. In 90 min after hypoxic exposure, the serum levels of HIF-1α, erythropoietin, VEGF, and TGF-ß returned to normal in high-resistant rats, while in low-resistant animals, an increase in 8-isoprostane and TGF-ß concentrations was observed. The rats with different resistance to hypoxia were characterized by different changes in biomolecular parameters determining predilection to inflammatory diseases.


Assuntos
Corticosterona/sangue , Dinoprosta/análogos & derivados , Eritropoetina/sangue , Subunidade alfa do Fator 1 Induzível por Hipóxia/metabolismo , Hipóxia/tratamento farmacológico , Fígado/metabolismo , NF-kappa B/metabolismo , Fator de Crescimento Transformador beta1/sangue , Fator A de Crescimento do Endotélio Vascular/metabolismo , Animais , Dinoprosta/sangue , Perfilação da Expressão Gênica , Regulação da Expressão Gênica , Subunidade alfa do Fator 1 Induzível por Hipóxia/sangue , Inflamação , Fígado/efeitos dos fármacos , Masculino , Ratos , Ratos Wistar , Fator A de Crescimento do Endotélio Vascular/sangue
15.
J Occup Environ Med ; 60(12): 1073-1081, 2018 12.
Artigo em Inglês | MEDLINE | ID: mdl-30188494

RESUMO

OBJECTIVES: The current laboratory study quantified blood oxidative stress to woodsmoke exposure. METHODS: Participants inhaled woodsmoke during three randomized crossover exercise trials (Clean Air [0 µg/m], Low Exposure [250 µg/m], and High Exposure [500 µg/m], Woodsmoke [particulate matter less than 2.5 µm, PM2.5]). Trolox equivalent antioxidant capacity (TEAC), uric acid (UA), 8-isoprostanes (8-ISO), lipid hydroperoxides (LOOH), protein carbonyls (PC), nitrotyrosine (3-NT), 8-isoprostane, and myeloperoxidase (MPO) were quantified in Pre, immediately Post, and 1- (1Hr) hour post blood samples. RESULTS: UA decreased following Low Exposure, while plasma TEAC levels increased Post and 1Hr. LOOH levels decreased 1Hr Post (High Exposure), while 8-Iso increased following both smoke trials. PC and MPO were unchanged following all trials, while 3-NT increased over Clean Air. CONCLUSION: Blood oxidative stress occurred largely independent of PM2.5 concentrations. Future studies should employ longer duration smoke and exercise combined with physiologic parameters.


Assuntos
Exposição por Inalação/efeitos adversos , Estresse Oxidativo , Esforço Físico , Fumaça/efeitos adversos , Adulto , Antioxidantes , Estudos Cross-Over , Dinoprosta/análogos & derivados , Dinoprosta/sangue , Teste de Esforço , Humanos , Isoprostanos/sangue , Peróxidos Lipídicos/sangue , Material Particulado/efeitos adversos , Peroxidase/sangue , Carbonilação Proteica , Distribuição Aleatória , Tirosina/análogos & derivados , Tirosina/sangue , Ácido Úrico/sangue , Incêndios Florestais , Madeira , Adulto Jovem
16.
Anticancer Res ; 38(9): 5211-5216, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30194169

RESUMO

AIM: To investigate the effect of oxidative stress biomarkers on the risk of potentially malignant oral disorders (PMODs). MATERIALS AND METHODS: A total of 208 male adults with PMODs and an equal number of same-age control patients were enrolled. Plasma biomarkers of oxidative stress, measured with 8-hydroxy-2'-deoxyguanosine (8-OHdG) and 8-isoprostane (8-ISO), were determined using enzyme-linked immunosorbent assay (ELISA) kits. PMODs were diagnosed in accordance with the World Health Organization (WHO) guidelines. RESULTS: A significant association between a high level of 8-ISO and an increased risk of PMODs was identified [odds ratio (OR)=1.71, 95% confidence interval (CI)=1.12-2.63; p=0.013]. This positive association was stronger among patients with PMOD subtype of leukoplakia (OR=1.94, 95% Cl=1.24-3.06; p=0.004). However, no significant association was observed between plasma 8-OHdG levels and overall risk of PMODs or subtypes. CONCLUSION: Increased plasma 8-ISO levels may indicate the prominence of lipid peroxidation in the development of PMODs, particularly leukoplakia.


Assuntos
Desoxiguanosina/análogos & derivados , Dinoprosta/análogos & derivados , Neoplasias Bucais/sangue , Lesões Pré-Cancerosas/sangue , Adulto , Biomarcadores/sangue , Desoxiguanosina/sangue , Dinoprosta/sangue , Humanos , Peroxidação de Lipídeos , Masculino , Pessoa de Meia-Idade , Estresse Oxidativo , Fatores de Risco
17.
Zhongguo Zhong Yao Za Zhi ; 43(16): 3341-3345, 2018 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-30200739

RESUMO

Blood stasis syndrome is the pre-state of thrombotic disease. The model of blood stasis syndrome in rats was induced by sleep deprivation to study on effects of blood stasis syndrome on platelet activation. The weight, the color of tongue and hemorheology for the blood stasis syndrome of Chinese medicine were measured after modeling. The release of platelet granules and platelet activation factors in plasma were detected by ELISA kit related indicators to provide experimental basis for platelet function evaluation and related drug effects in syndrome research. The results showed that the weight of the model group rats was significantly lower than that of the normal group (P<0.01). The tongue showed a dark purple blood stasis pattern, and the R, G and B values of the tongue surface in model group were significantly lower than those of the normal group (P<0.01). The hemorheological parameters including high shear, middle shear and low shear viscosity in whole blood were significantly higher than those in the normal group (P<0.01). But plasma viscosity did not change significantly. The release levels of platelet α particles (GMP-140, ß-TG, PF4) and dense particles (ADP, 5-HT) were significantly higher than those in the normal group (P<0.01). The levels of TXB2 and 6-keto-PGF1α in plasma were significantly higher than those in the normal group (P<0.01). The ratios of TXB2 and 6-keto-PGF2α were also significantly higher than those in the normal group (P<0.01). The levels of PAF in plasma in model group were significantly higher than those in the normal group (P<0.01). It was concluded that platelet functions could be changed induced by sleep deprivationin rats with blood stasis syndrome, and there might be inflammation and endothelial cell dysfunction.


Assuntos
Medicina Tradicional Chinesa , Ativação Plaquetária , Privação do Sono , Animais , Dinoprosta/sangue , Modelos Animais de Doenças , Hemorreologia , Fator de Ativação de Plaquetas/análise , Ratos , Proteínas com Domínio T/sangue , Trombose
18.
Complement Ther Med ; 40: 120-125, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30219436

RESUMO

OBJECTIVE: To evaluate the impact to oxidative stress, atherosclerosis and macrovascular disease by two proprietary herbal medicines including Ginkgo Leaf Tablets and Liuwei Dihuang Pills in type 2 diabetes. METHODS: The recruited 140 type 2 diabetes were randomly divided into the treatment group and control group which were both received basic diabetic management including anti-hyperglycemia, anti-hypertension, life style adjustment and health education etc. Additionally, the treatment group was given both Ginkgo Leaf Tablets and Liuwei Dihuang Pills while the control group was given placebos of Ginkgo Leaf Tablets and Liuwei Dihuang Pills. The relative clinical indexes about macrovascular events occurrence, atherosclerosis degree(IMT levels), oxidative stress in vivo(plasma carboxymethyl lysine(CML) and 8-isoprostane(8-IsoP) levels), plasma glucose, plasma lipid, blood pressure, other drugs usage situations and so on of two groups before and after consecutive 36-month treatment were accurately collected and statistically analyzed. RESULTS: There were no significant differences of cardiovascular disease, cerebrovascular disease, IMT levels, plasma CML and 8-IsoP levels between the two groups before treatment. After 36-month treatment, the plasma CML and 8-IsoP levels of treatment group were both significantly lower than control group (CML: 312.4 ± 90.4 ng/ml versus 463.5 ± 97.2 ng/ml, P < 0.0001; 8-IsoP: 23.7 ± 9.5 pg/ml versus 62.6 ± 16.1 pg/ml, P < 0.0001) although this improvement was not shared with IMT and macrovascular events. CONCLUSION: Ginkgo Leaf Tablets and Liuwei Dihuang Pills are beneficial to oxidative stress which plays important role in diabetic atherosclerosis and macrovascular complications. The preventive and therapeutic values of herbal medicines will be proved in further diabetic complication researches.


Assuntos
Diabetes Mellitus Tipo 2/tratamento farmacológico , Medicamentos de Ervas Chinesas/uso terapêutico , Extratos Vegetais/uso terapêutico , Idoso , Biomarcadores/sangue , Pressão Sanguínea/efeitos dos fármacos , Diabetes Mellitus Tipo 2/sangue , Dinoprosta/análogos & derivados , Dinoprosta/sangue , Medicamentos de Ervas Chinesas/farmacologia , Feminino , Humanos , Lisina/análogos & derivados , Lisina/sangue , Masculino , Pessoa de Meia-Idade , Estresse Oxidativo/efeitos dos fármacos , Extratos Vegetais/farmacologia
19.
J Dairy Sci ; 101(11): 10536-10556, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30197147

RESUMO

Objectives were to evaluate the effects of altering the dietary ratio of omega-6 (n-6) to omega-3 (n-3) fatty acids on the profile of fatty acids and expression of genes related to the prostaglandin biosynthesis on endometrial tissue, uterine secretion of PGF2α, and timing of spontaneous luteolysis in dairy cows. Multiparous lactating Holstein cows (n = 45) were blocked based on milk yield and, within each block, assigned randomly to 1 of 3 dietary treatments at 14 d postpartum for 90 d. Diets were supplemented with a mixture of Ca salts of fish, safflower, and palm oils to create 3 different ratios of n-6 to n-3 fatty acids, namely R4, R5, and R6, which resulted in 3.9, 4.9, and 5.9 parts of n-6 to 1 part of n-3 fatty acids, respectively. Blood was sampled every 2 h from d 16 to 23 of the estrous cycle and assayed for concentrations of progesterone and the PGF2α metabolite 13,14-dihydro-15-keto-PGF2α (PGFM). In a subsequent estrous cycle, endometrial tissue was collected for biopsy on d 8 and endometrial fatty acids profile and gene expression were quantified. The proportion of arachidonic acid of the endometrial fatty acids increased as the dietary ratio n-6 to n-3 fatty acids increased (R4 = 9.05, R5 = 11.64, and R6 = 13.41%). On the other hand, proportions of eicosapentaenoic (R4 = 2.85, R5 = 2.14, and R6 = 2.02%) and docosahexaenoic (R4 = 3.30, R5 = 1.57, and R6 = 1.08%) decreased as the ratio of n-6 to n-3 fatty acids in the diet increased. Increasing the ratio of dietary n-6 to n-3 fatty acids increased mRNA expression of estrogen receptor 1, oxytocin receptor, cyclooxygenase 2, prostaglandin E and F synthases, and steroidogenic acute regulatory protein in endometrium, but decreased expression of peroxisome proliferator-activated receptor gamma and insulin-like growth factor-1. The changes in endometrium gene expression caused by dietary treatments were associated with changes in the ratio of n-6 to n-3 fatty acids in the endometrium. As the ratio increased from R4 to R6, the number of PGFM pulses (R4 = 5.6, R5 = 4.3, and R6 = 3.8 ± 0.6 pulses; least squares means ± standard error of the means) decreased, but the amplitude of the greatest PGFM pulse increased (R4 = 226, R5 = 267, and R6 = 369 ± 38 pg/mL). Luteolysis by d 23 of the estrous cycle was observed in 79.6% of the cows (R4 = 11/14; R5 = 13/15; and R6 = 11/15) and day of spontaneous luteolysis did not differ among treatments (R4 = 20.8; R5 = 21.1; and R6 = 21.0 ± 0.4). Three pulses of PGFM was the best predictor of luteolysis in dairy cows. Collectively, supplying the same quantity of fatty acids in the diet of lactating dairy cows, but altering the ratio of n-6 to n-3 fatty acids, influenced the endometrial fatty acids profile and gene expression and altered the pattern of prostaglandin synthesis; however, the changes were not sufficient to alter the length of the estrous cycle.


Assuntos
Bovinos/fisiologia , Suplementos Nutricionais , Ácidos Graxos Ômega-3/farmacologia , Ácidos Graxos Ômega-6/farmacologia , Ácidos Graxos/metabolismo , Leite/metabolismo , Animais , Dieta/veterinária , Gorduras Insaturadas na Dieta/metabolismo , Dinoprosta/sangue , Endométrio/metabolismo , Ciclo Estral , Feminino , Regulação da Expressão Gênica/efeitos dos fármacos , Lactação , Luteólise/efeitos dos fármacos , Progesterona/sangue
20.
Eur J Pharmacol ; 836: 122-128, 2018 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-30096295

RESUMO

N-acetylgalactosaminyltransferase 2-Angiopoietin-like protein 3-lipoprotein lipase (GALNT2-ANGPTL3-LPL) pathway may be a useful pharmacologic objective for dyslipidemia. The present study was conducted to test the effect of paeoniflorin, a monoterpene Glycoside, on dyslipidemia in mice. Fifty mice were randomly divided into five groups (n = 10): three groups of apolipoprotein E-null (ApoE-/-) mice treated with paeoniflorin (10 or 20 or 30 mg/kg/day), untreated ApoE-/- mice group, and C57BL/6J control group. Six weeks after treatment, expression of hepatic ANGPTL3, hepatic GALNT2 and adipose tissue LPL, lipid levels in the liver and blood were quantified. Treatment with paeoniflorin (10 or 20 or 30 mg/kg) obviously down-regulated expression of ANGPTL3 and up-regulated expressions of GALNT2 and LPL concomitantly with elevated plasma high-density lipoprotein cholesterol level, reduced plasma concentrations of low-density lipoprotein cholesterol, total cholesterol, triglyceride, malonaldehyde, and 8-isoprostane. The present results suggest that paeoniflorin regulates GALNT2-ANGPTL3-LPL pathway to attenuate dyslipidemia in mice.


Assuntos
Proteínas Semelhantes a Angiopoietina/metabolismo , Dislipidemias/tratamento farmacológico , Dislipidemias/metabolismo , Glucosídeos/farmacologia , Lipase Lipoproteica/metabolismo , Monoterpenos/farmacologia , N-Acetilgalactosaminiltransferases/metabolismo , Proteínas Semelhantes a Angiopoietina/genética , Animais , Dinoprosta/análogos & derivados , Dinoprosta/sangue , Dislipidemias/genética , Dislipidemias/patologia , Regulação Enzimológica da Expressão Gênica/efeitos dos fármacos , Glucosídeos/uso terapêutico , Lipase Lipoproteica/genética , Fígado/efeitos dos fármacos , Fígado/metabolismo , Malondialdeído/sangue , Camundongos , Monoterpenos/uso terapêutico , N-Acetilgalactosaminiltransferases/genética , RNA Mensageiro/genética , RNA Mensageiro/metabolismo
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