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1.
Adv Exp Med Biol ; 1155: 415-427, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31468419

RESUMO

Maternal dyslipidemia induces metabolic and cardiovascular disorders in adult offspring. This study tests the hypothesis that perinatal taurine supplementation prevents the adverse effects of maternal dyslipidemia on growth and cardiovascular function in adult rat offspring. Female Wistar rats were fed normal rat chow and water with (Dyslipidemia) or without dyslipidemia induction (Control) by intraperitoneal Triton WR-1339 injection, three times a week for 4 weeks. The female Control and Dyslipidemia rats were supplemented with (Control+T, Dyslipidemia+T) or without 3% taurine in water from conception to weaning. After weaning, male and female offspring were fed normal rat chow and water throughout the experiment. At 16 weeks of age, body weights significantly increased in male but not female Dyslipidemia compared to other groups, while visceral fat content significantly increased in both male and female Dyslipidemia groups. Further, both sexes displayed similar high fasting blood sugar and normal plasma leptin levels among the groups. While plasma total cholesterol and triglycerides significantly increased only in female Dyslipidemia, low-density lipoprotein cholesterol increased in both male and female Dyslipidemia groups. Mean arterial pressures and heart rates significantly increased, while baroreflex sensitivity decreased in male and female Dyslipidemia compared to all other groups. High-density lipoprotein cholesterol did not significantly different among male or female groups. These changes of the male and female Dyslipidemia group were ameliorated by perinatal taurine supplementation. The present study indicates that perinatal taurine supplementation prevents the adverse effects of maternal dyslipidemia on growth and cardiovascular function in both male and female, adult offspring.


Assuntos
Suplementos Nutricionais , Dislipidemias/fisiopatologia , Fenômenos Fisiológicos da Nutrição Materna , Taurina/farmacologia , Animais , Barorreflexo , Pressão Sanguínea , LDL-Colesterol/sangue , Feminino , Frequência Cardíaca , Lipídeos/sangue , Masculino , Gravidez , Efeitos Tardios da Exposição Pré-Natal , Ratos , Ratos Wistar
2.
Medicine (Baltimore) ; 98(31): e16323, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31374004

RESUMO

The aim of this study was to conduct a more comprehensive analysis of the association between psoriasis and abnormal lipid metabolism.The case-control study included 222 psoriatic patients and 445 non-psoriatic control patients matched for age and gender. Clinical parameters included age, gender, and body mass index (BMI). Serum lipid levels were recorded and included cholesterol (CHO), triglycerides (TG), low-density lipoprotein (LDL), high density lipoprotein (HDL), phospholipids (PLIP), free fatty acids (FFA), lipoprotein (a) [Lp(a)], and apolipoproteins (apoA1, apoB, and apoE). Statistical analysis was carried out through the IBM Statistical Package for the Social Studies version 23.0.Compared with controls, levels of BMI and the prevalence of obesity were significantly higher in psoriatic patients. The results revealed that when compared to controls, significant elevation of serum TG (P <.001) and Lp(a) (P = .022) was observed. Levels of HDL (P <.001) and apoA1 (P <.001) were significantly lower in psoriatic patients. There was no significant difference in CHO (P = .367), LDL (P = .400), apoB (P = .294), apoE (P = .05), PLIP (P = .931) and FFA (P = .554) between patients and controls. The levels of CHO, TG, PLIP, FFA, and apoE were positively correlated with BMI level.Dyslipidemia was more common in psoriatic patients, compared with non-psoriatic controls.


Assuntos
Dislipidemias/etiologia , Obesidade/etiologia , Psoríase/complicações , Adulto , Idoso , Apolipoproteínas B/análise , Apolipoproteínas B/sangue , Apolipoproteínas E/análise , Apolipoproteínas E/sangue , Índice de Massa Corporal , Estudos de Casos e Controles , China/epidemiologia , LDL-Colesterol/análise , LDL-Colesterol/sangue , Dislipidemias/epidemiologia , Dislipidemias/fisiopatologia , Feminino , Humanos , Lisina Acetiltransferase 5/análise , Lisina Acetiltransferase 5/sangue , Masculino , Pessoa de Meia-Idade , Obesidade/epidemiologia , Obesidade/fisiopatologia , Psoríase/epidemiologia , Psoríase/fisiopatologia
3.
Diabetes Res Clin Pract ; 154: 90-100, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31238058

RESUMO

AIM: This study was aimed to evaluate the cardiomyopathy in patients with type 2 diabetes mellitus (T2DM) who live with or without cardiovascular complications by estimating different cardiac biomarkers. METHODS: This cross-sectional study enrolled 125 participants including 25 healthy volunteers and 100 T2DM patients. After meeting all inclusion criteria, the participants were categorized into five groups (N = 25 in each) as; healthy volunteers (I), T2DM (II), T2DM with hypertension (III), T2DM with dyslipidemia (IV), T2DM with hypertension and dyslipidemia (V). Pearson's correlation analysis was performed to assess the significant association between cardiac biomarkers other biochemical parameters. P-values <0.05 were considered statistically significant. RESULTS: The average age of the participants was found to be 55.04 ±â€¯7.51 years. The positive correlation was found between HbA1c and calcium or BNP levels however, a negative association was observed with zinc level. Group V showed higher mean of BNP (pg/mL) as 86.73 ±â€¯64.49 followed by Group III (61.02 ±â€¯53.69), IV (33.88 ±â€¯33.71), II (13.49 ±â€¯11.67) and I (5.54 ±â€¯1.49) which predicts the subclinical cardiomyopathies in the respective groups. Serum zinc (µg/dL) level were significantly lower in Group V (52.72 ±â€¯12.16) followed by III (56.15 ±â€¯9.64), IV (58.10 ±â€¯10.05), II (59.49 ±â€¯11.33) and I (73.96 ±â€¯21.91). CONCLUSIONS: In summary, BNP and calcium levels were significantly elevated while zinc was significantly reduced in T2DM patients with cardiovascular complication. Results from the study also shown positive correlation between BNP, calcium, Troponin-I levels and blood pressure. However, further longitudinal studies required to confirm these findings.


Assuntos
Biomarcadores/análise , Cardiomiopatias/diagnóstico , Diabetes Mellitus Tipo 2/complicações , Dislipidemias/fisiopatologia , Hipertensão/fisiopatologia , Cardiomiopatias/etiologia , Cardiomiopatias/metabolismo , Estudos de Casos e Controles , Estudos Transversais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Prognóstico
4.
Med Sci Monit ; 25: 3344-3353, 2019 May 06.
Artigo em Inglês | MEDLINE | ID: mdl-31059494

RESUMO

BACKGROUND A Chinese population-based study aimed to investigate the risk factors for the incidence and severity of drug-induced liver injury (DILI) from Chinese herbal medicines and conventional Western medicines. MATERIAL AND METHODS Liver biopsy and routine laboratory testing, including serum lipid measurements, was performed on 465 patients, including 168 patients with DILI and 297 patients without DILI. Histological grading of DILI used the METAVIR scoring system and the severity of DILI was graded as levels 0-5. Multivariate and univariate regression analysis were used to compare the two study groups, using a risk-adjusted odds ratio (AOR). RESULTS There was no significant association between age, alcohol status, cardiovascular disease (CVD), hypertension, or type 2 diabetes mellitus and development of DILI. However, when compared with controls, patients with dyslipidemia (AOR, 2.173; 95% CI, 1.388-3.401; P=0.001) had an increased incidence of DILI, and men had a reduced incidence of DILI when compared with women (AOR, 0.276; 95% CI, 0.169-0.450; P<0.001). Risk factors for severe DILI (≥level 3) included drinking alcohol (AOR, 6.506; 95% CI, 2.184-19.384; P=0.001), and dyslipidemia (AOR, 3.095; 95% CI, 1.345-7.123; P=0.008). Patients with an increased duration of drug treatment of >1 year had a reduced risk of developing severe DILI compared with patients with a medication duration of ≤1 month (AOR, 0.259; 95% CI, 0.084-0.802). CONCLUSIONS Increased risk of the incidence of DILI was significantly associated with female gender and dyslipidemia, and the risk of developing severe DILI was associated with drinking alcohol and dyslipidemia.


Assuntos
Doença Hepática Induzida por Substâncias e Drogas/etiologia , Doença Hepática Induzida por Substâncias e Drogas/metabolismo , Dislipidemias/fisiopatologia , Adulto , Grupo com Ancestrais do Continente Asiático/genética , China , Dislipidemias/genética , Feminino , Humanos , Incidência , Lipídeos/análise , Lipídeos/sangue , Fígado/efeitos dos fármacos , Fígado/lesões , Fígado/metabolismo , Masculino , Pessoa de Meia-Idade , Razão de Chances , Estudos Retrospectivos , Fatores de Risco , Índice de Gravidade de Doença
5.
Lipids Health Dis ; 18(1): 75, 2019 Mar 28.
Artigo em Inglês | MEDLINE | ID: mdl-30922333

RESUMO

BACKGROUND: Previous studies have reported that an irregular work schedule, particularly nighttime work, is associated with an altered lipid profile. Additionally, a mismatch in circadian rhythm can affect sleeping and eating habits, leading to poor health. This study aimed to examine the association between night work and dyslipidemia among South Korean adults aged ≥30 years. METHODS: For this study, the data of 5813 participants in the 2013-2016 Korea National Health and Nutrition Examination Survey were analyzed. Diagnoses of dyslipidemia were based on blood sampling tests of total cholesterol, high-density lipoprotein (HDL), low-density lipoprotein (LDL) cholesterol, and triglyceride levels. Night work was defined as that conducted during evening (6 P.M.-12 A.M.) and overnight hours (12 A.M.-8 A.M.). The association between night work hours and dyslipidemia in South Korean men and women was investigated using a logistic regression analysis. RESULTS: After adjusting for sociodemographic, economic, health-related, and nutritional factors, an association of night work with dyslipidemia was observed in male participants (odds ratio = 1.53, 95% confidence interval: 1.05-2.24). In subset analyses of male participants, night workers who skipped meals were more likely to have dyslipidemia than their day-working counterparts. Among men who slept < 7 h, night workers had a higher probability of dyslipidemia than day workers. In contrast, no statistically significant association between night work and dyslipidemia was observed in female participants, although the probability of dyslipidemia appeared to increase with advancing age. Furthermore, when women with dyslipidemia were subdivided by occupational categories, night workers in white collar positions were more likely to have dyslipidemia than their day-working counterparts. CONCLUSION: Our study observed an association of night work with dyslipidemia, particularly in men. Although these findings may support interventions for South Korean night workers, further studies are needed for validation.


Assuntos
Dislipidemias/sangue , Lipídeos/sangue , Jornada de Trabalho em Turnos/efeitos adversos , Higiene do Sono , Adulto , Idoso , Colesterol/sangue , Ritmo Circadiano/genética , Dislipidemias/epidemiologia , Dislipidemias/fisiopatologia , Comportamento Alimentar , Feminino , Humanos , Lipoproteínas HDL/sangue , Lipoproteínas LDL/sangue , Masculino , Pessoa de Meia-Idade , República da Coreia , Fatores de Risco , Sono/fisiologia , Higiene do Sono/fisiologia , Fatores Socioeconômicos , Triglicerídeos/sangue
6.
Pediatr Cardiol ; 40(4): 805-812, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30759268

RESUMO

Atherosclerosis promoting cardiovascular disease risk factors (CVDrf) are highly prevalent among youth in the U.S. Determining which standard modifiable clinical measures (SMCMs) has the greatest impact on vascular structure and function is valuable for the health care provider to help identify children at highest risk. The aim of this study was to determine modifiable outpatient clinical predictors of vascular health in youth with CVDrf. Children and adolescents with CVDrf (n = 120, 13.1 ± 1.9 years, 49% female) were recruited from a pediatric preventive cardiology clinic. The SMCMs included BMI z-score, waist-to-height ratio (WTHR), lipid panel, hemoglobin A1c, blood pressure (BP), presence of tobacco smoke exposure, and presence of hypertriglyceridemic waist (HTW) phenotype (triglycerides ≥ 110 mg/dL and waist circumference ≥ 90 percentile). Vascular function and structure were measured with pulse wave velocity (PWV), central systolic BP (CSP), augmentation index (AIx), and carotid artery intima-media thickness (cIMT). Sex and height specific z-scores for PWV, CSP, and cIMT were used. Multiple linear regression with backwards selection identified SMCMs which strongly predicted vascular function and structure. Among SMCMs, WTHR and HTW were the most frequent predictors of vascular function (PWV: R2 = 0.32; CSP: R2 = 0.35; AIx R2 = 0.13). Other predictors of vascular function included hemoglobin A1C, BP, and BMI z-score. Systolic BP and LDL cholesterol were predictors of vascular structure (cIMT: R2 = 0.14). The strongest predictors of vascular health in youth with CVDrf were related to measures of central obesity. Targeting these SMCM in lieu of vascular testing in outpatient clinic setting may be practical to identify children and adolescents at greatest risk for CVD.


Assuntos
Vasos Sanguíneos/fisiopatologia , Doenças Cardiovasculares/etiologia , Dislipidemias/fisiopatologia , Adolescente , Pressão Sanguínea/fisiologia , Doenças Cardiovasculares/diagnóstico , Espessura Intima-Media Carotídea/estatística & dados numéricos , Criança , Dislipidemias/complicações , Feminino , Humanos , Lipídeos/sangue , Masculino , Análise de Onda de Pulso/métodos , Fatores de Risco
8.
Expert Opin Investig Drugs ; 28(4): 389-394, 2019 04.
Artigo em Inglês | MEDLINE | ID: mdl-30753100

RESUMO

INTRODUCTION: Despite the substantial reduction in cardiovascular morbidity and mortality after the management of dyslipidemia with statins, residual risk remains even after achieving low-density lipoprotein cholesterol targets. This residual risk appears to be partly attributed to low levels of high-density lipoprotein cholesterol (HDL-C) and high levels of triglycerides (TG). Apolipoprotein C3 (APOC3) is a key regulator of TG metabolism and its targeting may reduce TG levels and cardiovascular risk. AREAS COVERED: We discuss APOC3-targeted experimental treatments for dyslipidemia. There is an emphasis on volanesorsen because it the agent in the most advanced stage of development. M580, a retinoic acid receptor-α specific agonist, an agent in early-stage development is briefly covered. Preclinical data suggest that this agent decreases APOC3 mRNA levels and reduces total cholesterol, TG levels and hepatic lipid accumulation. EXPERT OPINION: The effects of this novel therapeutic approach on cardiovascular morbidity and mortality should be determined in randomized controlled trials. The cost of volanesorsen, the unfavorable safety profile and the need for subcutaneous administration present barriers to long-term use. AM580 may hold promise in the management of hypertriglyceridemia but further investigations are necessary to evaluate safety and efficacy.


Assuntos
Apolipoproteína C-III/metabolismo , Hiperlipidemias/tratamento farmacológico , Oligonucleotídeos/uso terapêutico , Animais , Apolipoproteína C-III/genética , HDL-Colesterol/sangue , Dislipidemias/tratamento farmacológico , Dislipidemias/fisiopatologia , Humanos , Hiperlipidemias/fisiopatologia , Lipídeos/sangue , Oligonucleotídeos/efeitos adversos , Oligonucleotídeos/farmacologia , RNA Mensageiro/metabolismo , Ensaios Clínicos Controlados Aleatórios como Assunto , Triglicerídeos/sangue
9.
Diabetes Metab Syndr ; 13(1): 56-61, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30641765

RESUMO

BACKGROUND: The World Health Organization recommends the implementation of interventions focused on the early detection of clinical risk factors for cardiovascular disease (CVD) as effective strategies for the control of CVD in low resource settings. However, due to health system resource constraints, surveillance capacity for the identification of high-risk populations for non-communicable diseases, including CVD have been inadequate. The purpose of this study was to describe the prevalence of CVD clinical risk factors among healthy adults residing in the Cape Coast metropolis of Ghana. The clinical risk factors assessed included glycemic control, insulin sensitivity, lipid control and blood pressure. METHODS: The study participants included 70 healthy adults without a previous diagnosis of CVD from Cape Coast metropolis. Blood samples, blood pressure and anthropometric measurement were obtained for each participant. Serum glycated hemoglobin (HbA1c), insulin, glucose, triglycerides, and cholesterol levels were measured. RESULTS: Approximately four out of ten participants were either overweight or obese. Almost three-quarters of the sample were considered prehypertensive or hypertensive. About three in ten were clinically prediabetic. About a third of the participants had high non-HDL cholesterol levels. Triglyceride concentration levels were found to be high in almost 10 percent of the study sample. Approximately six percent were identified as having metabolic syndrome. CONCLUSION: A significant proportion of the study participants were identified to be at risk for CVD. There is the need for adaptive and less resource-intensive CVD risk-factor screening interventions to allow for the timely detection and management of CVD risk factors in low-resource settings.


Assuntos
Pressão Sanguínea , Doenças Cardiovasculares/epidemiologia , Diabetes Mellitus/fisiopatologia , Dislipidemias/fisiopatologia , Resistência à Insulina , Síndrome Metabólica/fisiopatologia , Obesidade/fisiopatologia , Adulto , Idoso , Biomarcadores/análise , Feminino , Seguimentos , Gana/epidemiologia , Humanos , Masculino , Pessoa de Meia-Idade , Prognóstico
10.
Lipids Health Dis ; 18(1): 10, 2019 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-30621702

RESUMO

BACKGROUND: Maonan nationality is a relatively conservative and isolated minority in the Southwest of China. Little is known about the association of endothelial lipase gene (LIPG) single nucleotide polymorphisms (SNPs) and serum lipid levels in the Chinese populations. METHODS: A total of 1280 subjects of Maonan nationality and 1218 participants of Han nationality were randomly selected from our previous stratified randomized samples. Genotypes of the four LIPG SNPs were determined by polymerase chain reaction-restriction fragment length polymorphism, and then confirmed by direct sequencing. RESULTS: Several SNPs were associated with high-density lipoprotein cholesterol (rs3813082, rs2000813 and rs2097055) in the both ethnic groups; total cholesterol and apolipoprotein (Apo) A1 (rs2000813) in Han nationality; and low-density lipoprotein cholesterol, ApoB, triglyceride (rs2097055) and ApoA1 (rs3819166) in Maonan minority (P < 0.0125 for all after Bonferroni correction). The commonest haplotype was rs3813082T-rs2000813C-rs2097055T-rs3819166A (Han, 44.2% and Maonan, 48.7%). The frequencies of the T-C-T-A, T-C-T-G, T-T-C-G and G-T-C-G haplotypes were different between the Maonan and Han populations (P < 0.05-0.001). The associations between haplotypes and dyslipidemia were also different in the Han and/or Maonan populations (P < 0.05-0.001). CONCLUSIONS: The differences in serum lipid profiles between the two ethnic groups might partly be attributed to these LIPG SNPs, their haplotypes and gene-environmental interactions. TRIAL REGISTRATION: Retrospectively registered.


Assuntos
Dislipidemias/etnologia , Dislipidemias/genética , Interação Gene-Ambiente , Lipase/genética , Polimorfismo de Nucleotídeo Único , Adulto , Idoso , Idoso de 80 Anos ou mais , Apolipoproteína A-I/sangue , Apolipoproteína A-I/genética , Apolipoproteína B-100/sangue , Apolipoproteína B-100/genética , China/epidemiologia , HDL-Colesterol/sangue , LDL-Colesterol/sangue , Dislipidemias/sangue , Dislipidemias/fisiopatologia , Grupos Étnicos , Expressão Gênica , Estudos de Associação Genética , Haplótipos , Humanos , Lipase/sangue , Masculino , Pessoa de Meia-Idade , Triglicerídeos/sangue
11.
Artigo em Inglês | MEDLINE | ID: mdl-30626137

RESUMO

Metabolic syndrome (MetS) is a cluster of cardiometabolic risk factors. Many people may be unaware of their risk for MetS. A cross-sectional, descriptive study was conducted among hospitalized patients with at least one cardiometabolic risk factor in Mainland China. This study assessed the MetS knowledgelevel(through MetS Knowledge Scale, MSKS) and examined the potential predictors by regression analysis. A total of 204 patients aged 58.5 ± 10.1 years (55% males) participated in this study. The majority of participants had no history of hypertension (54%), dyslipidemia (79%), or diabetes (85%). However, 56% of these participants had at least three cardiometabolic risk factors, indicating the presence of MetS. The average MSKS was very low (mean = 36.7±18.8, possible range = 0⁻100), indicating the urgent needs of MetS education in current practice. Predictors of better MetS knowledge included higher educational level, history of dyslipidemia, and normal high-density lipoprotein cholesterol (F (8, 195) = 9.39, adjusted R² = 0.192, p< 0.001). In conclusion, adults with cardiometabolic risk factors are at risk of developing MetS, but with a low level of knowledge. Specific health education on MetS should be provided, particularly for those with limited formal education or inadequate lipid management.


Assuntos
Síndrome Metabólica/epidemiologia , Adulto , Idoso , China , HDL-Colesterol/sangue , Estudos Transversais , Dislipidemias/fisiopatologia , Feminino , Humanos , Hipertensão/fisiopatologia , Masculino , Síndrome Metabólica/fisiopatologia , Pessoa de Meia-Idade , Prevalência , Fatores de Risco , Adulto Jovem
12.
Clin Calcium ; 29(2): 237-243, 2019.
Artigo em Japonês | MEDLINE | ID: mdl-30679406

RESUMO

Age-related osteoporosis and atherosclerosis is promoted by life style-related diseases such as dyslipidemia and diabetes mellitus. Common factors pathophysiologically involved in both osteoporosis and vascular calcification include senescent cells and osteoprotegerin(OPG). Dyslipidemia may impair both osteoclast and osteoblast function,thereby causing osteoporosis. Statins may have favorable effect on bone. Some anti-osteoporotic medications have also been suggested to show protective effect from atherosclerosis.


Assuntos
Aterosclerose , Dislipidemias , Osteoporose , Calcificação Vascular , Aterosclerose/fisiopatologia , Dislipidemias/fisiopatologia , Humanos , Osteoclastos , Osteoporose/fisiopatologia , Osteoprotegerina/fisiologia , Calcificação Vascular/metabolismo
13.
Pathology ; 51(2): 165-176, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30598326

RESUMO

Apolipoprotein E (apoE), a 34 kDa circulating glycoprotein of 299 amino acids, predominantly synthesised in the liver, associates with triglyceride-rich lipoproteins to mediate the clearance of their remnants after enzymatic lipolysis in the circulation. Its synthesis in macrophages initiates the formation of high density-like lipoproteins to effect reverse cholesterol transport to the liver. In the nervous system apoE forms similar lipoproteins which perform the function of distributing lipids amongst cells. ApoE accounts for much of the variation in plasma lipoproteins by three common variants (isoforms) that influence low-density lipoprotein concentration and the risk of atherosclerosis. ApoE2 generally is most favourable and apoE4 least favourable for cardiovascular and neurological health. The apoE variants relate to different amino acids at positions 112 and 158: cysteine in both for apoE2, arginine at both sites for apoE4, and respectively cysteine and arginine for apoE3 that is viewed as the wild type. Paradoxically, under metabolic stress, homozygosity for apoE2 may result in dysbetalipoproteinaemia in adults owing to impaired binding of remnant lipoproteins to the LDL receptor and related proteins as well as heparan sulphate proteoglycans. This highly atherogenic condition is also seen with other mutations in apoE, but with autosomal dominant inheritance. Mutations in apoE may also cause lipoprotein glomerulopathy. In the central nervous system apoE binds amyloid ß-protein and tau protein and fragments may incur cellular damage. ApoE4 is a strong risk factor for the development of Alzheimer's disease. ApoE has several other physiological effects that may influence health and disease, including supply of docosahexaenoic acid for the brain and modulating immune and inflammatory responses. Genotyping of apoE may have application in disorders of lipoprotein metabolism as well as glomerulopathy and may be relevant to personalised medicine in understanding cardiovascular risk, and the outcome of nutritional and therapeutic interventions. Quantitation of apoE will probably not be clinically useful. ApoE is also of interest as it may generate peptides with biological function and could be employed in nanoparticles that may allow crossing of the blood-brain barrier. Therapeutic options may emerge from these newer insights.


Assuntos
Apolipoproteínas E/metabolismo , Doenças Cardiovasculares/metabolismo , Dislipidemias/metabolismo , Metabolismo dos Lipídeos , Lipoproteínas/metabolismo , Sistema Nervoso/metabolismo , Peptídeos beta-Amiloides/metabolismo , Apolipoproteína E2/genética , Apolipoproteína E2/metabolismo , Apolipoproteína E4/genética , Apolipoproteína E4/metabolismo , Apolipoproteínas E/genética , Doenças Cardiovasculares/fisiopatologia , Dislipidemias/fisiopatologia , Saúde , Proteoglicanas de Heparan Sulfato/metabolismo , Humanos , Lipoproteínas/genética , Fígado/metabolismo , Sistema Nervoso/fisiopatologia , Isoformas de Proteínas , Receptores de LDL/genética , Receptores de LDL/metabolismo , Fatores de Risco
14.
Endocrinol. diabetes nutr. (Ed. impr.) ; 66(1): 4-10, ene. 2019. tab, graf
Artigo em Inglês | IBECS | ID: ibc-175787

RESUMO

Introduction: Patients with HIV+ often present lipid disturbances. The role of ghrelin and obestatin in these lipid disturbances is not clear. The effect of antiretroviral (ART) drugs on those molecules is also unknown. This study measured ghrelin and obestatin levels, as well as metabolic markers, in patients with HIV+ before and after 36 weeks of ART. Material and methods: Twenty HIV-positive, ART-naïve patients who started a scheme consisting of tenofovir/emtricitabine+lopinavir/ritonavir were enrolled. Plasma samples were collected before and after 36 weeks of treatment. Serum ghrelin and obestatin levels were quantitated by ELISA; glucose, cholesterol, and triglyceride levels were measured by colorimetric and enzymatic methods, and cardiovascular risk was calculated by the atherogenic index of plasma (AIP). Results: All patients completed 36 weeks of ART. Total cholesterol (p<0.001), LDL-C (p=0.019), HDL-C (p=0.003), VLDL-C (p=0.002), and triglyceride levels (p=0.021) significantly increased after treatment. AIP revealed increased cardiovascular risk at baseline, which remained high after treatment. There was a statistically significant increase in obestatin level in the unpaired and paired analyses, while ghrelin levels only showed a trend to increase. Changes in ghrelin and obestatin levels positively correlated, but no correlation was seen with any metabolic parameter. Conclusion: After 36 weeks of ART, patients showed an altered lipid profile, but there were no significant changes in cardiovascular risk. Ghrelin and obestatin levels increased after 36 weeks of ART, but the increase was only significant for obestatin. Changes in ghrelin and obestatin positively correlate


Introducción: Los pacientes con VIH+ frecuentemente presentan alteraciones del perfil lípidico. El papel de ghrelina y obestatina en estas complicaciones no está claro. El efecto del tratamiento antirretroviral (TAR) en dichas moléculas es desconocido. Este estudio determinó los niveles de ghrelina y obestatina, así como los parámetros metabólicos en pacientes VIH+ antes y después de 36 semanas del TAR. Material y métodos: Participaron 20 pacientes VIH+, vírgenes a TAR, que iniciaron con un esquema de tenofovir/emtricitabina + lopinavir/ritonavir. Se tomaron muestras de plasma antes y después de 36 semanas de tratamiento. Los niveles séricos de ghrelina y obestatina fueron cuantificados por ELISA, los parámetros bioquímicos fueron determinados por métodos colorimétricos, se evaluó el riesgo cardiovascular por medio del índice aterogénico del plasma (AIP). Resultados: Los pacientes completaron 36 semanas del TAR. Los niveles de colesterol total (p<0,001), c-LDL (p=0,019), c-HDL (p=0,003), c-VLDL (p=0,002) y triglicéridos (p=0,021) mostraron un incremento estadísticamente significativo posterior al tratamiento. El AIP reveló un riesgo cardiovascular alto. Los niveles de obestatina se incrementaron significativamente en el análisis pareado y no pareado; y ghrelina solo mostró tendencia al incremento. Los cambios en ghrelina y obestatina correlacionaron positivamente, sin embargo no correlacionaron con los parámetros metabólicos. Conclusión: Los pacientes VIH+ mostraron un perfil lipídico alterado después de 36 semanas del TAR. Los niveles de ghrelina y obestatina se incrementaron tras 36 semanas del TAR. El riesgo cardiovascular es persistente. Los cambios en ghrelina y obestatina mostraron una correlación positiva


Assuntos
Humanos , Masculino , Feminino , Adulto , Antirretrovirais/farmacologia , HIV , Grelina/sangue , Hormônios Peptídicos/sangue , Antirretrovirais/uso terapêutico , HIV/metabolismo , Grelina/uso terapêutico , Hormônios Peptídicos/uso terapêutico , Dislipidemias/fisiopatologia
15.
J Sports Sci ; 37(1): 50-58, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-29882716

RESUMO

This study aimed to investigate if moderate to vigorous physical activity (MVPA) and aerobic fitness are associated with cardiovascular risk factors in HIV+ children and adolescents. Sixty-five children and adolescents (8 to 15 years) provided minutes of MVPA measured by accelerometers and peak oxygen uptake (peak VO2) by breath-by-breath respiratory exchange. Cardiovascular risk factors were characterized by body fat, blood pressure, total cholesterol, HDL-c, LDL-c, triglycerides, glucose, insulin, C-reactive protein (CRP), interleukin (IL)-6, tumor necrosis factor-alpha (TNF-α) and carotid intima-media thickness. Results indicated that higher MVPA was associated with lower values of total (ß =  -3.566) and trunk body fat (ß = -3.495), total cholesterol (ß = -0.112) and LDL-c (ß = -0.830). Likewise, higher peak VO2 was associated with lower total (ß = -0.629) and trunk body fat values (ß = -0.592) and levels of CRP (ß = -0.059). The physically active participants had lower total cholesterol (-24.4 mg.dL-1) and LDL-c (-20.1 mg.dL-1) compared to participants judged to be insufficiently active. Moreover, participants with satisfactory peak VO2 showed lower total (-4.1%) and trunk (-4.3%) body fat, CRP (-2.3 mg.L-1), IL-6 (-2.4 pg.mL-1) and TNF-α (-1.0 pg.mL-1) compared to low peak VO2 peers. High levels of MVPA and aerobic fitness may prevent developing of cardiovascular risk factors in children and adolescents HIV+.


Assuntos
Adiposidade , Aptidão Cardiorrespiratória , Dislipidemias/fisiopatologia , Exercício/fisiologia , Infecções por HIV/fisiopatologia , Mediadores da Inflamação/sangue , Acelerometria , Adolescente , Glicemia/metabolismo , Pressão Sanguínea , Doenças Cardiovasculares/fisiopatologia , Espessura Intima-Media Carotídea , Criança , Estudos Transversais , Feminino , Infecções por HIV/sangue , Humanos , Lipídeos/sangue , Masculino , Consumo de Oxigênio , Troca Gasosa Pulmonar , Fatores de Risco
16.
Clin Investig Arterioscler ; 31(2): 56-62, 2019.
Artigo em Inglês, Espanhol | MEDLINE | ID: mdl-30591270

RESUMO

INTRODUCTION: Wistar and Sprague Dawley (SD) rats are generally used as models for the cholesterol metabolism experiments. They are acceptable to high fat diet-induced disorders with individual variations, including dyslipidemia and abnormal cardiac pathology. OBJECTIVE: To compare the effects of high fat diet in inducing dyslipidemia and cardiac pathological alterations between Wistar and SD rats. METHODS: We compared the differences in plasma cholesterol levels and cardiac pathological alterations between Wistar and SD rats of standard diet (3.90 kcal/g) and high fat diet (5.40 kcal/g) after 4 weeks. RESULTS: SD rats fed with high fat diet showed significantly enhanced LDL concentration and the decreased HDL concentration when compared to Wistar rats. Additionally, SD rats showed cardiac pathological alterations such as infiltration of mononuclear cells referring to inflammatory response and high amounts of perivascular fat playing a key role in the impairment of vascular functions. CONCLUSIONS: Our results indicate that SD rats may be the more suitable model for dyslipidemia and alteration of cardiac pathology induced by high fat diet.


Assuntos
Colesterol/sangue , Dieta Hiperlipídica/efeitos adversos , Dislipidemias/fisiopatologia , Cardiopatias/fisiopatologia , Animais , HDL-Colesterol/sangue , LDL-Colesterol/sangue , Modelos Animais de Doenças , Masculino , Ratos , Ratos Sprague-Dawley , Ratos Wistar , Especificidade da Espécie
17.
BMC Med Genet ; 19(1): 205, 2018 11 29.
Artigo em Inglês | MEDLINE | ID: mdl-30497399

RESUMO

BACKGROUND: The objective of this study was to examine individual and community factors that influence high-density lipoprotein cholesterol (HDL-C) dyslipidemia in Newfoundland and Labrador (NL), a genetically isolated population in Canada with a high prevalence of HDL-C dyslipidemia. METHODS: First, a group of single nucleotide polymorphisms from 10 metabolic trait candidate genes was tested using a multivariate logistic regression model. The significant SNPs were entered into the second phase, where a mixed logistic model incorporated the community disease risk factors together with the individual factors as the fixed part of the model and the geographic region as a random effect. RESULTS: Analysis of 1489 subjects (26.9% HDL-C dyslipidemia) identified rs3758539, a non-coding variant in the 5'UTR of RBP4, to be associated with HDL-C dyslipidemia (odds ratio = 1.45, 95% confidence interval = 1.08-1.97, p = 0.01). The association remained significant, and the effect size did not change after the incorporation of individual and community risk factors from 17 geographic regions (odds ratio: 1.41, 95% confidence interval = 1.03-1.93, p = 0.03) in NL. Besides this variant, sex, BMI, and smoking also showed significant associations with HDL-C dyslipidemia, whereas no role was identified for the community factors. CONCLUSIONS: This study demonstrates the use of community-level data in a genetic association testing. It reports a functional variant in the promoter of RBP4, a gene directly involved in lipoprotein metabolism, to be associated with HDL-C dyslipidemia. These findings indicate that individual factors are the main reason for a higher prevalence of HDL-C dyslipidemia in the NL population.


Assuntos
HDL-Colesterol/sangue , Dislipidemias/genética , Efeito Fundador , Modelos Genéticos , Proteínas Plasmáticas de Ligação ao Retinol/genética , Regiões 5' não Traduzidas , Adulto , Índice de Massa Corporal , HDL-Colesterol/deficiência , Dislipidemias/sangue , Dislipidemias/epidemiologia , Dislipidemias/fisiopatologia , Feminino , Expressão Gênica , Estudos de Associação Genética , Humanos , Masculino , Pessoa de Meia-Idade , Terra Nova e Labrador/epidemiologia , Razão de Chances , Polimorfismo de Nucleotídeo Único , Prevalência , Regiões Promotoras Genéticas , Isolamento Reprodutivo , Proteínas Plasmáticas de Ligação ao Retinol/metabolismo , Fatores de Risco , Fatores Sexuais , Fumar/genética , Fumar/fisiopatologia
18.
J Sex Med ; 15(12): 1678-1697, 2018 12.
Artigo em Inglês | MEDLINE | ID: mdl-30527052

RESUMO

BACKGROUND: Previous work showed that muscle-derived stem cells (MDSCs) exposed long-term to the milieu of uncontrolled type 2 diabetes (UC-T2D) in male obese Zucker (OZ) rats, were unable to correct the associated erectile dysfunction and the underlying histopathology when implanted into the corpora cavernosa, and were also imprinted with a noxious gene global transcriptional signature (gene-GTS), suggesting that this may interfere with their use as autografts in stem cell therapy. AIM: To ascertain the respective contributions of dyslipidemia and hyperglycemia to this MDSC damage, clarify its mechanism, and design a bioassay to identify the damaged stem cells. METHODS: Early diabetes MDSCs and late diabetes MDSCs were respectively isolated from nearly normal young OZ rats and moderately hyperglycemic and severely dyslipidemic/obese aged rats with erectile dysfunction. Monolayer cultures of early diabetic MDSCs were incubated 4 days in DMEM/10% fetal calf serum + or - aged OZ or lean Zucker serum from non-diabetic lean Zucker rats (0.5-5%) or with soluble palmitic acid (PA) (0.5-2 mM), cholesterol (CHOL) (50-400 mg/dL), or glucose (10-25 mM). MAIN OUTCOME MEASURE: Fat infiltration was estimated by Oil red O, apoptosis by TUNEL, protein expression by Western blots, and gene-GTS and microRNA (miR)-GTS were determined in these stem cells' RNA. RESULTS: Aged OZ serum caused fat infiltration, apoptosis, myostatin overexpression, and impaired differentiation. Some of these changes, and also a proliferation decrease occurred with PA and CHOL. The gene-GTS changes by OZ serum did not resemble the in vivo changes, but some occurred with PA and CHOL. The miR-GTS changes by OZ serum, PA, and CHOL resembled most of the in vivo changes. Hyperglycemia did not replicate most alterations. CLINICAL IMPLICATIONS: MDSCs may be damaged in long-term UC-T2D/obese patients and be ineffective in autologous human stem cell therapy, which may be prevented by excluding the damaged MDSCs. STRENGTH & LIMITATIONS: The in vitro test of MDSCs is innovative and fast to define dyslipidemic factors inducing stem cell damage, its mechanism, prevention, and counteraction. Confirmation is required in other T2D/obesity rat models and stem cells (including human), as well as miR-GTS biomarker validation as a stem cell damage biomarker. CONCLUSION: Serum from long-term UC-T2D/obese rats or dyslipidemic factors induces a noxious phenotype and miR-GTS on normal MDSCs, which may lead in vivo to the repair inefficacy of late diabetic MDSCs. This suggests that autograft therapy with MDSCs in long-term UT-T2D obese patients may be ineffective, albeit this may be predictable by prior stem cell miR-GTS tests. Masouminia M, Gelfand R, Kovanecz I, et al. Dyslipidemia Is a Major Factor in Stem Cell Damage Induced by Uncontrolled Long-Term Type 2 Diabetes and Obesity in the Rat, as Suggested by the Effects on Stem Cell Culture. J Sex Med 2018;15:1678-1697.


Assuntos
Diabetes Mellitus Experimental/fisiopatologia , Dislipidemias/complicações , Disfunção Erétil/etiologia , Transplante de Células-Tronco , Animais , Diferenciação Celular , Diabetes Mellitus Experimental/terapia , Dislipidemias/fisiopatologia , Disfunção Erétil/fisiopatologia , Humanos , Masculino , Obesidade/complicações , Pênis/fisiopatologia , Ratos , Ratos Zucker
19.
Lipids Health Dis ; 17(1): 278, 2018 Dec 06.
Artigo em Inglês | MEDLINE | ID: mdl-30522496

RESUMO

BACKGROUND: Numerous studies reported that dyslipidemia was associated with cancer risk. However, few studies investigated the associations between dyslipidemia and non-small cell lung cancer (NSCLC). METHODS: Four hundred twenty-four histologically confirmed NSCLC cases and 414 controls, matched for age and sex, were enrolled to examine the relationship between dyslipidemia and NSCLC. Demographic and clinical data were obtained from patients' medical records and telephone interviews. Odds ratios (ORs) and 95% confidence intervals (CIs) were estimated using unconditional logistic regression. RESULTS: Abnormal triglyceride (TG) and high-density lipoprotein cholesterol (HDL-C) levels showed statistically significant coexistence with NSCLC compared with controls. Higher levels of TG were associated with a higher risk of NSCLC (OR = 1.541, 95% CI, (1.072-2.215)). The odds ratios (ORs) for NSCLC for normal and high levels of HDL-C versus those with a low level of HDL-C were 0.337(95% CI, (0.242-0.468)) and 0.288(95% CI, (0.185-0.448)), respectively. After adjustment for age, sex, smoking status, hypertension, body mass index, diabetes and lipid profiles, the adjusted OR for normal and high levels of HDL-C were 0.320(95% CI, (0.218-0.470)) and 0.233(95% CI, (0.134-0.407)), respectively. However, after adjustment, high levels of TG increased the risk of NSCLC but not significantly (OR = 1.052, 95% CI (0.671-1.649)). CONCLUSIONS: This study provided evidence that dyslipidemia increased the risk of NSCLC in Chinese population.


Assuntos
Carcinoma Pulmonar de Células não Pequenas/epidemiologia , Dislipidemias/epidemiologia , Hipertensão/epidemiologia , Adulto , Idoso , Índice de Massa Corporal , Carcinoma Pulmonar de Células não Pequenas/sangue , Carcinoma Pulmonar de Células não Pequenas/complicações , Carcinoma Pulmonar de Células não Pequenas/patologia , Estudos de Casos e Controles , China/epidemiologia , HDL-Colesterol/sangue , Dislipidemias/sangue , Dislipidemias/complicações , Dislipidemias/fisiopatologia , Feminino , Humanos , Hipertensão/sangue , Hipertensão/complicações , Hipertensão/fisiopatologia , Masculino , Pessoa de Meia-Idade , Fatores de Risco , Fumar , Triglicerídeos/sangue
20.
Sci Rep ; 8(1): 16276, 2018 11 02.
Artigo em Inglês | MEDLINE | ID: mdl-30390007

RESUMO

Dyslipidemia is associated with glomerular injury. However, the effect of statins on chronic kidney disease (CKD) progression remains controversial. We aimed to investigate the efficacy of statins for renal protection in patients with CKD. The retrospective cohort study comprised 3441 patients diagnosed with CKD in multiple medical centers. We divided the patients into two cohorts based on statin prescription, and compared proportions and risks of CKD progression events between the two groups. CKD progression event was defined as an average annual decline of eGFR >5 mL/min/1.73 m2 or advancement to the dialysis stage. The result revealed that among all incident patients with CKD, 28.7% and 30.3% of the users and nonusers demonstrated CKD progression, respectively. The crude odds ratio (OR) of CKD progression was 0.93 [95% confidence interval (CI) 0.78-1.10]. After adjustment for baseline characteristics, the adjusted OR was 0.80 (95% CI 0.63-1.01). The sensitivity analysis results showed consistent OR for CKD progression, stratification by age, sex, Charlson score, and statins use within 1 year before index date. The effect of statins was significant in patients with CKD stage 3B-5 (OR 0.68, 95% CI 0.48-0.95), but not statistically significant in those with CKD stage 1-3A (OR 0.97, 95% CI 0.68-1.38). The effect of statins was significant in patients with proteinuria ≥1000 mg/day (OR 0.63, 95% CI 0.43-0.92), but not statistically significant in those with proteinuria <1000 mg/day (OR 1.02, 95% CI 0.74-1.41).


Assuntos
Dislipidemias/tratamento farmacológico , Taxa de Filtração Glomerular/efeitos dos fármacos , Inibidores de Hidroximetilglutaril-CoA Redutases/administração & dosagem , Proteinúria/prevenção & controle , Insuficiência Renal Crônica/prevenção & controle , Adulto , Fatores Etários , Idoso , Bases de Dados Factuais/estatística & dados numéricos , Progressão da Doença , Dislipidemias/complicações , Dislipidemias/fisiopatologia , Dislipidemias/urina , Feminino , Seguimentos , Humanos , Rim/efeitos dos fármacos , Rim/fisiopatologia , Masculino , Pessoa de Meia-Idade , Proteinúria/etiologia , Proteinúria/fisiopatologia , Proteinúria/urina , Insuficiência Renal Crônica/etiologia , Insuficiência Renal Crônica/fisiopatologia , Insuficiência Renal Crônica/urina , Estudos Retrospectivos , Fatores Sexuais , Adulto Jovem
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