Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 17.313
Filtrar
1.
Recurso na Internet em Português | LIS - Localizador de Informação em Saúde | ID: lis-LISBR1.1-46971

RESUMO

Apresenta uma cartilha em quadrinhos com informações sobre os vetores da doença de chagas desenvolvida pela equipe do Laboratório Nacional e Internacional de Referência em Taxonomia de Triatomíneos do Instituto Oswaldo Cruz.


Assuntos
Doença de Chagas , Triatominae
2.
Bull Entomol Res ; 110(1): 169-176, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31337451

RESUMO

Transmission of Trypanosma cruzi (Kinetoplastida: Trypanosomatidae) occurs when feces/urine of infected triatomines come into contact with mucous membranes or damaged skin, and this occurs mainly when insects defecate while feeding on the host. Thus, the vector competence of the triatomines is associated with their feeding and excretion/defecation behavior. This work studied for the first time the effect of T. cruzi infection on feeding and excretion/defecation patterns of Triatoma infestans (Hemiptera: Reduviidae). Uninfected and infected fifth-instar nymphs were fed ad libitum and their feeding behavior and defecations were registered during and after feeding. The feeding pattern did not show differences between the experimental groups. However, the infected nymphs began to defecate earlier, defecated in greater quantity and there was a greater proportion of defecating individuals compared to uninfected nymphs. These results show that T. cruzi affected the excretion/defecation pattern of T. infestans in a way that would increase the probability of contact between infective feces and the mammalian host.


Assuntos
Interações Hospedeiro-Parasita , Triatoma/parasitologia , Trypanosoma cruzi/fisiologia , Animais , Doença de Chagas/transmissão , Defecação , Comportamento Alimentar , Triatoma/fisiologia
3.
Acta Trop ; 201: 105188, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31545949

RESUMO

Triatoma petrocchiae is the newly member of the Triatoma brasiliensis species complex. This species overlaps with T. brasiliensis in geographic and ecotypic occupation in the sylvatic habitat because both inhabit rocky outcrops in the semi-arid portion of Brazilian northeast. In this region T. brasiliensis is the most important Chagas disease vector because it constantly colonizes domiciles. In contrast, T. petrocchiae is rarely found in peri or intradomiciliary habitats - reason why little is known about this species. Therefore, Here, we present information for the first time on. the T. petrocchiae ecotopes, genetic diversity, Trypanosoma cruzi prevalence/genotyping in comparison to T. brasiliensis. We found T. brasilensis (N = 223) and T. petrocchiae (N = 69) in co-habitation in rocky outcrops in three Districts of Paraíba and Rio Grande do Norte states. Forty-tree T. petrocchiae insects of eleven sampling spots (composing three geographic populations) were genotyped for the mitochondrial Cyt B gene and little geographic structure was observed. Tajima's D test suggested that species is evolving toward a mutation-drift equilibrium in our collection range. Sylvatic T. petrocchiae had 4% (3/68) of infected insects by T. cruzi, whereas T. brasiliensis had 26% (59/223). Fluorescent Fragment Length Barcoding demonstrated that all three T. petrocchiae harbored TcI whereas T. brasiliensis had TcI, but also TcIII, TcII/TcVI and T. rangeli genotype A, sometimes under mixed infections. None of infected T. petrocchiae were carrying mixed infections. However, this result should be confirmed using a larger pool of infected bugs. We here presented the first documentation of T. rangeli infecting T. brasiliensis. The finding of infected T. petrocchiae calls for constant vector monitoring because the epidemiologic scenario is dynamic and sylvatic vectors are progressively found in adaptation to anthropic environments.


Assuntos
Doença de Chagas/transmissão , Insetos Vetores/parasitologia , Simpatria , Triatoma/parasitologia , Trypanosoma cruzi/genética , Animais , Brasil/epidemiologia , Doença de Chagas/epidemiologia , Ecossistema , Variação Genética , Genótipo , Epidemiologia Molecular , Prevalência
4.
Acta Trop ; 201: 105218, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31610148

RESUMO

Despite more than 100 years since it was firstly described Chagas disease, only two drugs are available to treat Chagas disease: Nifurtimox launched by Bayer in 1965 and benznidazole launched by Roche in 1971. Drug discovery initiatives have been looking for new compounds as an alternative to these old drugs. Although new platforms have been used with the latest technologies, a critical step on that process still relies on the in vivo model. Unfortunately, to date, available animal models have limited predictive value and there is no standardization. With the aim to better understand the role of benznidazole, the current standard of care of Chagas disease, we performed this review. We intend to analyze the influence of the experimental design of the most used animal model, the murine model, in the assessment of the efficacy endpoint.


Assuntos
Doença de Chagas/tratamento farmacológico , Nifurtimox/farmacologia , Nifurtimox/uso terapêutico , Nitroimidazóis/farmacologia , Nitroimidazóis/uso terapêutico , Tripanossomicidas/uso terapêutico , Trypanosoma cruzi/efeitos dos fármacos , Animais , Modelos Animais de Doenças , Relação Dose-Resposta a Droga , Humanos , Camundongos
5.
J Enzyme Inhib Med Chem ; 35(1): 21-30, 2020 12.
Artigo em Inglês | MEDLINE | ID: mdl-31619095

RESUMO

Trypanosoma cruzi carbonic anhydrase (TcCA) has recently emerged as an interesting target for the design of new compounds to treat Chagas disease. In this study we report the results of a structure-based virtual screening campaign to identify novel and selective TcCA inhibitors. The combination of properly validated computational methodologies such as comparative modelling, molecular dynamics and docking simulations allowed us to find high potency hits, with KI values in the nanomolar range. The compounds also showed trypanocidal effects against T. cruzi epimastigotes and trypomastigotes. All the candidates are selective for inhibiting TcCA over the human isoform CA II, which is encouraging in terms of possible therapeutic safety and efficacy.


Assuntos
Inibidores da Anidrase Carbônica/farmacologia , Anidrases Carbônicas/metabolismo , Doença de Chagas/tratamento farmacológico , Ciclamatos/farmacologia , Tripanossomicidas/farmacologia , Inibidores da Anidrase Carbônica/síntese química , Inibidores da Anidrase Carbônica/química , Doença de Chagas/metabolismo , Ciclamatos/síntese química , Ciclamatos/química , Relação Dose-Resposta a Droga , Avaliação Pré-Clínica de Medicamentos , Humanos , Modelos Moleculares , Estrutura Molecular , Testes de Sensibilidade Parasitária , Relação Estrutura-Atividade , Tripanossomicidas/síntese química , Tripanossomicidas/química , Trypanosoma cruzi/efeitos dos fármacos , Trypanosoma cruzi/enzimologia
6.
Rev Soc Bras Med Trop ; 52: e20180541, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31800918

RESUMO

INTRODUCTION: Chagas disease is a major public health problem that is endemic in Brazil and Latin America. This study aimed to determine the socioeconomic, demographic, and clinical characteristics of 171 patients (mean age, 45 years; female, 65%) with Chagas disease at Hospital Universitário de Brasília, Federal District, Brazil. METHODS: We implemented this cross-sectional study using a clinical epidemiological questionnaire, electrocardiography, echocardiography, and quantitative detection of Trypanosoma cruzi DNA in blood using qRT-PCR. RESULTS: Among the patients, 26.3% had a full elementary education, and 13.2% were illiterate. Most (63.6%) were economically classified as class C, and 51.5% were born in Bahia state. A total of 62.0% participants reported previous contact with the triatomine bug. The clinical forms of the disease were indeterminate (69.51%), cardiac (15.24%), digestive (10.37%), and mixed (4.88%). The most common electrocardiographic abnormality was complete right bundle branch block in association with a divisional anterosuperior block. Only 14.6% of the patients complied with benznidazole medication for at least 60 days, and 164 of them were assessed by echocardiography. The parasite load was positive in 56% of the patients. CONCLUSIONS: Chagas disease affected mostly women, with the indeterminate chronic form of the disease.


Assuntos
Doença de Chagas/epidemiologia , Trypanosoma cruzi/genética , Adulto , Idoso , Brasil/epidemiologia , Doença de Chagas/parasitologia , Estudos Transversais , DNA de Protozoário/genética , Ecocardiografia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Carga Parasitária , Reação em Cadeia da Polimerase em Tempo Real , Fatores Socioeconômicos , Trypanosoma cruzi/isolamento & purificação , Adulto Jovem
7.
Artigo em Inglês | MEDLINE | ID: mdl-31859844

RESUMO

Although the salivary glands present several functions, there are few studies evaluating these glands in Chagas disease (CD). This study aimed to compare the percentage of collagen, the presence of inflammation, the density of chimase and tryptase mast cells, the area and density of lingual salivary gland acini in autopsied individuals with and without (CD). We analyzed 400 autopsy reports performed in a tertiary public hospital from 1999 to 2015 and selected all the cases in which tongue fragments were collected (27 cases), 12 with chronic CD without megaesophagus (CH) and 15 without CD (non-chagasic - NC). The histological sections of the tongue were stained by Picrosirius red for collagen evaluation and Hematoxylin-eosin for morphometric evaluation of salivary gland acini and inflammation. Anti-chimase and anti-tryptase antibodies were used for the immunohistochemical evaluation of mast cells. The chagasic patients presented higher volume and lower density of salivary glands acini. There was no difference in the collagen percentage, inflammation and density of mast cell chymase and tryptase between the groups. Although we did not observe a significant difference between the groups regarding the collagen percentage, inflammatory process and mast cell density, our results suggest that even without megaesophagus, chagasic patients present hypertrophy of the lingual salivary glands and lower acinar density probably due to mechanisms independent of the esophagus-glandular stimulus.


Assuntos
Células Acinares/patologia , Doença de Chagas/patologia , Glândulas Salivares/patologia , Língua/patologia , Idoso , Doença Crônica , Feminino , Humanos , Hipertrofia , Imuno-Histoquímica , Masculino
8.
Rev Med Chil ; 147(6): 683-692, 2019 Jun.
Artigo em Espanhol | MEDLINE | ID: mdl-31859820

RESUMO

BACKGROUND: Reproductive number (R0)-maps estimate risk zones of vector-borne diseases and geographical distribution changes under climate change. AIM: To map R0 aiming to estimate the epidemiological risk of Chagas disease in Chile, its distribution and possible changes due to the global climate change. MATERIAL AND METHODS: We used a relationship between R0 and entomological parameters of vectors as function of environmental variables, to map the risk of Chagas disease in Chile, under current and projected future environmental conditions. RESULTS: We obtained a geographical R0 estimation of Chagas disease in Chile. The highest R0averages correspond to the Central-Northern regions of Chile. T. cruzi transmission area could increase in the future due to climate changes. Independent of the future condition, both for optimistic and pessimistic climate change scenarios, the area of potential risk for Chagas disease transmission would increase. The estimated R0 values suggest that, if a control of T. infestans is not maintained, Chagas disease endemic status will persist or increase, independently of the climate change scenarios. CONCLUSIONS: Mapping R0 values is an effective method to assess the risk of Chagas disease. The eventual increase in the transmission area of the disease is worrisome.


Assuntos
Doença de Chagas/epidemiologia , Mudança Climática/estatística & dados numéricos , Vetores de Doenças , Medição de Risco/métodos , Animais , Dióxido de Carbono , Doença de Chagas/transmissão , Chile/epidemiologia , Feminino , Geografia , Humanos , Masculino , Fatores de Risco , Estatísticas não Paramétricas , Temperatura Ambiente , Triatoma , Trypanosoma cruzi
9.
Rev Soc Bras Med Trop ; 53: e20190119, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31859942

RESUMO

INTRODUCTION: Oral transmission of acute Chagas disease is an emerging public health concern. This study aimed to detect insect fragments in experimentally contaminated food, by comparing triatomines with other insects. METHODS: Food samples were experimentally contaminated with insects, processed to recover their fragments by light filth, and analyzed by microscopy and Polymerase Chain Reaction (PCR). RESULTS: Morphological differences between coleopteran and triatomine insects were observed in microscopic images. PCR was efficient in amplifying Triatominae DNA in the experimentally contaminated food. CONCLUSIONS: This methodology could be utilized by food analysts to identify possible insect contamination in food samples.


Assuntos
Besouros/classificação , Parasitologia de Alimentos , Sucos de Frutas e Vegetais/parasitologia , Insetos Vetores/classificação , Saccharum/parasitologia , Triatominae/classificação , Animais , Doença de Chagas/transmissão , Humanos , Reação em Cadeia da Polimerase
11.
Mem. Inst. Invest. Cienc. Salud (Impr.) ; 17(3): 10-19, dic.2019. tab, ilus
Artigo em Espanhol | LILACS, BDNPAR | ID: biblio-1047708

RESUMO

En Paraguay se estima que 165.000 personas están infectadas por Trypanosoma cruzi, agente etiológico de la enfermedad de Chagas, de las cuales 61.000 son mujeres en edad fértil. En 1995 se implementó el Programa de Control Prenatal de Chagas en el departamento de Cordillera, zona endémica del país. El objetivo de este estudio es analizar el comportamiento de la seropositividad a T. cruzi en embarazadas en 21 años de control prenatal (período: 1995-2016), con énfasis en el periodo 2010-2016. El estudio, descriptivo de corte trasversal retrospectivo, empleó los datos obtenidos de los registros de la III Región Sanitaria y de los cinco laboratorios de diagnóstico. Se emplearon los resultados de la serología de 20 distritos en un total de 23.661 embarazadas que asistieron a su control prenatal. En los registros figuran 1.074 embarazadas seropositivas, distribuidas en los cinco laboratorios de referencia, resultando el 5 % la prevalencia de infección con T. cruzi en el período 2010-2016. Con una prevalencia al inicio del período de 16 %, se evidenció el principal descenso a 6 % en el 2014, situación que se mantuvo en una fase cuasi estacionaria hasta el 2016. Se concluye que el sistema de diagnóstico prenatal de la enfermedad de Chagas asociado a los métodos de prevención implementados por el Ministerio de Salud Pública y Bienestar Social, han resultado efectivos. Esta información es útil para, a través de la identificación de las embarazadas seropositivas y el tratamiento correspondiente, controlar la transmisión congénita(AU)


It is estimated that in Paraguay 165,000 people are infected with Trypanosoma cruzi, the etiological agent of Chagas disease. Among them 61,000 are women of childbearing age. In 1995, it was implemented the Chagas Prenatal Control Program in the Cordillera department, one of the endemic areas of the country. Herein is analyzed the evolution of T. cruzi seropositivity in pregnant women in the 21 years of prenatal control (period: 1995-2016), with emphasis on the period 2010-2016. In this descriptive study, data from the registers of the III Sanitary Region and from the five diagnostic laboratories were used. The serologic results of 20 districts involved 23,661 pregnant women who attended their prenatal control. It was recorded 1,074 seropositive women in the five reference laboratories, resulting in a 5 % prevalence of infection with T. cruzi in the period 2010-2016. At the beginning of the Program the prevalence was 16 %, the main decrease, to 6 %, was evidenced in 2014 maintaining quasi stationary until 2016. The Chagas disease prenatal diagnosis system and the protocols of prevention by the Ministry of Public Health and Social Welfare were effective. This information is useful to control the congenital transmission through the identification of positive pregnant women and providing the adequate treatment(AU)


Assuntos
Humanos , Feminino , Gravidez , Estudos Soroepidemiológicos , Doença de Chagas , Cuidado Pré-Natal , Trypanosoma cruzi
13.
BMC Infect Dis ; 19(1): 956, 2019 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-31706289

RESUMO

BACKGROUND: Current available treatments (benznidazole and nifurtimox) for Chagas disease (CD) show limited efficacy in chronic phase and frequent undesirable effects. Ergosterol synthesis inhibitors (ESI) had been considered as promising drugs for CD treatment and despite its recent poor results in several clinical trials, different strategies have been proposed to optimize its role in this infection. CASE PRESENTATION: We present a case of chronic Chagas disease in patient diagnosed with HIV who received treatment for histoplasmosis with itraconazol during twelve months. Even though T. cruzi rt-PCR was persistently negative during treatment, when itraconazol was stopped she presented with a positive blood rt-PCR. CONCLUSION: Several studies using different ESI had been published for CD treatment. Either in vitro or in vivo assays demonstrated activity against T. cruzi of the different triazole derivatives so different clinical trials had been carried out to evaluate its efficacy and safety. Despite contradictory evidence in the animal model, longer treatments along with other treatment strategies previously proposed suggests that ESI failure rates in positive peripheral blood rt-PCR are higher than that obtained with the current treatments of choice.


Assuntos
Doença de Chagas/tratamento farmacológico , Itraconazol/uso terapêutico , Tripanossomicidas/uso terapêutico , Adulto , Animais , Contagem de Linfócito CD4 , Doença de Chagas/complicações , Doença Crônica , Feminino , Infecções por HIV/complicações , Infecções por HIV/diagnóstico , Humanos , Itraconazol/farmacologia , Tomografia Computadorizada por Raios X , Tripanossomicidas/farmacologia , Trypanosoma cruzi/efeitos dos fármacos
14.
Lancet ; 394(10209): 1592, 2019 11 02.
Artigo em Inglês | MEDLINE | ID: mdl-31690431
15.
Rev Soc Bras Med Trop ; 52: e20190020, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31778398

RESUMO

INTRODUCTION: Different blood meal sources can affect biology of triatomines. METHODS: We studied the influence of two different blood meal sources (rabbits and hens) on five biological parameters in Meccus phyllosomus pallidipennis and collected biological parameters. RESULTS: The cohort fed on rabbits had a shorter median life-cycle and lower mortality rate than that fed on hens. Both cohorts required a similar number of blood meals to reach adulthood. Median longevity and number of blood meals for adults were similar between both cohorts. CONCLUSIONS: The studied parameters reflect the high grade of adaptation of M. p. pallidipennis feeding on different hosts.


Assuntos
Comportamento Alimentar/fisiologia , Insetos Vetores/fisiologia , Estágios do Ciclo de Vida/fisiologia , Triatominae/fisiologia , Animais , Doença de Chagas/transmissão , Galinhas , Coelhos
16.
Rev Soc Bras Med Trop ; 52: e20190270, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31778401

RESUMO

INTRODUCTION: Trypanosomes can infect humans and animals. This is the first record of the occurrence of Trypanosoma evansi in Rondônia. METHODS: Blood samples were collected from 7 dogs and 22 humans. Furthermore, triatomines and tabanids were collected. RESULTS: It was observed that 42.8% of the dogs tested positive for T. evansi and 14.3% presented mixed infection; 15% of the triatomines tested positive for flagellates identified as T. cruzi TCI (3 specimens), T. cruzi TCI, and T. rangeli (1 specimen), and one with T. cruzi TCV. Two tabanids were infected with T. theileri. CONCLUSIONS: These findings may benefit vector control strategies.


Assuntos
Anticorpos Antiprotozoários/sangue , Doença de Chagas/epidemiologia , Insetos Vetores/parasitologia , Rhodnius/parasitologia , Trypanosoma/isolamento & purificação , Animais , Brasil/epidemiologia , Doença de Chagas/diagnóstico , Doença de Chagas/parasitologia , Cães , Inquéritos Epidemiológicos , Humanos , Trypanosoma/classificação
17.
Rev Soc Bras Med Trop ; 52: e20190278, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31778421

RESUMO

INTRODUCTION: Trypanosoma cruzi is the etiological agent of Chagas disease (CD), a zoonotic infection transmitted by triatomine bug vectors to human beings. Although the story of this parasitic infection was born in Brazil and here this has made major step forward information, the same cannot be said about the actual distribution of the triatomine vector in several areas of this country. The aim of this study was to assess the occurrence of triatomine species in an endemic region for CD in Northeastern Brazil. METHODS: A retrospective study was performed using data obtained from 2008 to 2017. All information was provided by the V Gerência Regional de Saúde of the state of Pernambuco. The spatial distribution of triatomine species was analyzed by drawing a map using the Quantum geographic information system. RESULTS: A total of 4,694 triatomine specimens (469.4 ± 221.2 per year) were collected during the period 2008-2017, with 94.5% (4,434/4,694) at the intradomicile and 5.5% (260/4,694) at peridomicile environment. Of all arthropods collected, 92.5% (4,340/4,694) and 7.5% (354/4,694) were adults and nymphs, respectively. The species most frequently detected were Panstrongylus lutzi (30.36%), Triatoma brasiliensis (26.12%), Triatoma pseudomaculata (22.43%), and Panstrongylus megistus (20.54%). CONCLUSIONS: These data contribute to a better understanding of the distribution of T. cruzi infection in the Northeastern region of Brazil. Preventive measures based on vector control should be implemented in the study area in order to reduce the burden this neglected tropical disease.


Assuntos
Distribuição Animal , Insetos Vetores/classificação , Triatominae/classificação , Animais , Brasil , Doença de Chagas/transmissão , Doenças Endêmicas , Densidade Demográfica , Estudos Retrospectivos
18.
Biochimie ; 167: 207-216, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31628976

RESUMO

Oligopeptidases B (OPB) belong to the S9 prolyl oligopeptidase family and are expressed in prokaryotes, some eukaryotes and in some higher plants. OPB is not found in any of the mammalian genomes available to date. Evidences indicate that OPB participates in the infections caused by trypanosomatids Trypanosoma cruzi, Leishmania spp. and Trypanosoma brucei spp and therefore it is considered an important virulence factor. Trypanosomatids from the genera Leishmania and Trypanosoma also present other OPB, named OPB2. A more accurate investigation of trypanosomatid OPB sequences brought attention to what could be a third OPB sequence (OPB3). This review aims to discuss biochemical, structural, phylogenetic and functional properties of OPB and its potential as target for the development of drugs against Chagas disease, leishmaniasis and African trypanosomiasis.


Assuntos
Leishmania/enzimologia , Serina Endopeptidases , Trypanosoma brucei brucei/enzimologia , Trypanosoma cruzi/enzimologia , Fatores de Virulência , Animais , Doença de Chagas/parasitologia , Humanos , Leishmaniose/parasitologia , Mamíferos , Proteínas de Protozoários/química , Proteínas de Protozoários/imunologia , Serina Endopeptidases/química , Serina Endopeptidases/classificação , Serina Endopeptidases/imunologia , Tripanossomíase Africana/parasitologia , Fatores de Virulência/química , Fatores de Virulência/classificação , Fatores de Virulência/imunologia
20.
Parasit Vectors ; 12(1): 478, 2019 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-31610815

RESUMO

BACKGROUND: Mepraia gajardoi and Mepraia spinolai are endemic triatomine vector species of Trypanosoma cruzi, a parasite that causes Chagas disease. These vectors inhabit arid, semiarid and Mediterranean areas of Chile. Mepraia gajardoi occurs from 18° to 25°S, and M. spinolai from 26° to 34°S. Even though both species are involved in T. cruzi transmission in the Pacific side of the Southern Cone of South America, no study has modelled their distributions at a regional scale. Therefore, the aim of this study is to estimate the potential geographical distribution of M. spinolai and M. gajardoi under current and future climate scenarios. METHODS: We used the Maxent algorithm to model the ecological niche of M. spinolai and M. gajardoi, estimating their potential distributions from current climate information and projecting their distributions to future climatic conditions under representative concentration pathways (RCP) 2.6, 4.5, 6.0 and 8.5 scenarios. Future predictions of suitability were constructed considering both higher and lower public health risk situations. RESULTS: The current potential distributions of both species were broader than their known ranges. For both species, climate change projections for 2070 in RCP 2.6, 4.5, 6.0 and 8.5 scenarios showed different results depending on the methodology used. The higher risk situation showed new suitable areas, but the lower risk situation modelled a net reduction in the future potential distribution areas of M. spinolai and M. gajardoi. CONCLUSIONS: The suitable areas for both species may be greater than currently known, generating new challenges in terms of vector control and prevention. Under future climate conditions, these species could modify their potential geographical range. Preventive measures to avoid accidental human vectorial transmission by wild vectors of T. cruzi become critical considering the uncertainty of future suitable areas projected in this study.


Assuntos
Doença de Chagas/transmissão , Mudança Climática , Insetos Vetores/fisiologia , Triatominae/fisiologia , Trypanosoma cruzi/fisiologia , Animais , Área Sob a Curva , Doença de Chagas/epidemiologia , Chile/epidemiologia , Humanos , Umidade , Insetos Vetores/parasitologia , Modelos Biológicos , Filogeografia , Curva ROC , Chuva , Medição de Risco , Temperatura Ambiente , Triatominae/parasitologia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA