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2.
Am J Clin Pathol ; 153(1): 139-145, 2020 01 01.
Artigo em Inglês | MEDLINE | ID: mdl-31584611

RESUMO

OBJECTIVES: We evaluated trends in non-Lyme disease tick-borne disease (NLTBI) testing at a national reference laboratory. METHODS: Testing data performed at Quest Diagnostics during 2010 to 2016 were analyzed nationally and at the state level. RESULTS: Testing and positivity for most NLTBIs increased dramatically from 2010 through 2016 based on testing from a large reference laboratory. The number of positive cases, though not as stringent as criteria for public health reporting, generally exceeds that reported by the Centers for Disease Control and Prevention. The frequency of NLTBI in the US is seasonal but testing activity and positive test results are observed throughout all months of the year. Positive results for NLTBI testing mostly originated from a limited number of states, indicating the geographic concentration and distribution of NLTBIs reported in this study. CONCLUSIONS: This report provides an important complementary source of data to best understand trends in and spread of NLTBI.


Assuntos
Notificação de Doenças , Doenças Transmitidas por Carrapatos/diagnóstico , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Anaplasmose/sangue , Anaplasmose/diagnóstico , Babesiose/sangue , Babesiose/diagnóstico , Criança , Pré-Escolar , Febre do Carrapato do Colorado/sangue , Febre do Carrapato do Colorado/diagnóstico , Ehrlichiose/sangue , Ehrlichiose/diagnóstico , Monitoramento Epidemiológico , Humanos , Lactente , Recém-Nascido , Pessoa de Meia-Idade , Febre Recorrente/sangue , Febre Recorrente/diagnóstico , Febre Maculosa das Montanhas Rochosas/sangue , Febre Maculosa das Montanhas Rochosas/diagnóstico , Doenças Transmitidas por Carrapatos/sangue , Tularemia/sangue , Tularemia/diagnóstico , Adulto Jovem
3.
BMC Infect Dis ; 19(1): 861, 2019 Oct 17.
Artigo em Inglês | MEDLINE | ID: mdl-31623574

RESUMO

BACKGROUND: Exposure to blacklegged ticks Ixodes scapularis that transmit pathogens is thought to occur peri-domestically. However, the locations where people most frequently encounter infected ticks are not well characterized, leading to mixed messages from public health officials about where risk is highest. METHODS: We conducted a systematic review and meta-analysis on spatial risk factors for tick-borne disease and tick bites in eastern North America. We examined three scales: the residential yard, the neighborhood surrounding (but not including) the yard, and outside the neighborhood. Nineteen eligible studies represented 2741 cases of tick-borne illness and 1447 tick bites. Using random effects models, we derived pooled odds ratio (OR) estimates. RESULTS: The meta-analysis revealed significant disease risk factors at the scale of the yard (OR 2.60 95% CI 1.96 - 3.46), the neighborhood (OR 4.08 95% CI 2.49 - 6.68), and outside the neighborhood (OR 2.03 95% CI 1.59 - 2.59). Although significant risk exists at each scale, neighborhood scale risk factors best explained disease exposure. Analysis of variance revealed risk at the neighborhood scale was 57% greater than risk at the yard scale and 101% greater than risk outside the neighborhood. CONCLUSIONS: This analysis emphasizes the importance of understanding and reducing tick-borne disease risk at the neighborhood scale. Risk-reducing interventions applied at each scale could be effective, but interventions applied at the neighborhood scale are most likely to protect human health. TRIAL REGISTRATION: The study was registered with PROSPERO: CRD42017079169 .


Assuntos
Picadas de Carrapatos/diagnóstico , Doenças Transmitidas por Carrapatos/diagnóstico , Animais , Humanos , Ixodes/fisiologia , Doença de Lyme/diagnóstico , Doença de Lyme/epidemiologia , América do Norte/epidemiologia , Razão de Chances , Características de Residência/estatística & dados numéricos , Fatores de Risco , Picadas de Carrapatos/epidemiologia , Doenças Transmitidas por Carrapatos/epidemiologia
4.
Ticks Tick Borne Dis ; 10(6): 101278, 2019 10.
Artigo em Inglês | MEDLINE | ID: mdl-31477530

RESUMO

North Asian tick-typhus (NATT), also known as Siberian tick typhus, is the main tick-borne rickettsiosis in Siberia, Russia. Recently, a fatal infection in a four-year-old girl with typical tick-borne rickettsiosis symptoms (fever, rash, eschar at the site of the tick bite, myalgia) and meningeal syndrome was registered. In order to identify the etiology of this infection, blood and brain samples from the patient were examined for the presence of a wide range of tick-transmitted agents and enteric viruses by polymerase chain reaction with subsequent sequencing. Rickettsia sibirica subsp. sibirica and "Candidatus Rickettsia tarasevichiae" DNA was identified in both blood and brain samples. Rickettsia sibirica was characterized by the gltA, ompA and ompB genes, and "Candidatus R. tarasevichiae" was characterized by the gltA and ompB genes. We report the first case of mixed R. sibirica and "Candidatus R. tarasevichiae" human infection with a fatal outcome in Russia.


Assuntos
Coinfecção/microbiologia , Rickettsia/isolamento & purificação , Rickettsiose do Grupo da Febre Maculosa/microbiologia , Doenças Transmitidas por Carrapatos/microbiologia , Pré-Escolar , Coinfecção/diagnóstico , Evolução Fatal , Feminino , Humanos , Sibéria , Rickettsiose do Grupo da Febre Maculosa/diagnóstico , Doenças Transmitidas por Carrapatos/diagnóstico
6.
Adv Clin Exp Med ; 28(7): 937-943, 2019 07.
Artigo em Inglês | MEDLINE | ID: mdl-31374162

RESUMO

BACKGROUND: Infections caused by tick-borne pathogens such as Bartonella spp., Borrelia burgdorferi s.l., Coxiella burnetii, and Rickettsia spp. are capable of causing serious lesions of the mitral and aortic valves, leading to a need for valve replacement. OBJECTIVES: The aim of the study was to determine whether such cases are sporadic or frequent. An additional goal was to establish effective diagnostic methods to detect these infections. MATERIAL AND METHODS: The study involved 148 patients undergoing valve replacement. Blood samples were drawn for serological testing. Samples of the removed mitral and aortic valves were tested with polymerase chain reaction and immunohistochemical staining. RESULTS: Specific antibodies to Bartonella spp. were detected in 47 patients (31.7%) and in 1 of the healthy controls (1%) (p < 0.05). Antibodies to B. burgdorferi spirochetes were found in 18 of the patients (12.2%) and in 6 blood donors from the control group (5.8%) (p < 0.1). Antibodies to Rickettsia spp. were detected in 12 (8.1%) and to C. burnetii phase I and II antigens in the serum of 1 patient. All the participants in the control group were seronegative to C. burnetii and Rickettsia spp. antigens. Polymerase chain reaction (PCR) tests for detection of Bartonella spp., B. burgdorferi s.l., C. burnetii and Rickettsia spp. DNA in the valve samples were all negative. Inflammation foci with mononuclear lymphoid cells in the aortic and mitral valves were seen in sections stained with hematoxiline and eozine. In sections dyed using the indirect immunofluorescence method with hyperimmune sera, Bartonella spp. and Rickettsia spp. were found. CONCLUSIONS: The results obtained indicate that laboratory diagnostics for patients with heart disorders should be expanded to include tests detecting tick-borne zoonoses such as bartonelloses, Lyme borreliosis, rickettsioses and Q fever.


Assuntos
Valva Aórtica/microbiologia , Infecções Bacterianas/microbiologia , Bartonella/isolamento & purificação , Borrelia/isolamento & purificação , Coxiella burnetii/isolamento & purificação , Endocardite/microbiologia , Valva Mitral/microbiologia , Rickettsia/isolamento & purificação , Doenças Transmitidas por Carrapatos/complicações , Animais , Bartonella/genética , Borrelia/classificação , Borrelia/genética , Estudos de Casos e Controles , Coxiella burnetii/genética , Endocardite/sangue , Humanos , Reação em Cadeia da Polimerase em Tempo Real , Rickettsia/classificação , Rickettsia/genética , Rickettsiales/classificação , Rickettsiales/genética , Rickettsiales/isolamento & purificação , Doenças Transmitidas por Carrapatos/sangue , Doenças Transmitidas por Carrapatos/diagnóstico , Carrapatos
8.
Artigo em Inglês | MEDLINE | ID: mdl-31300132

RESUMO

Infectious haemolytic anaemia (IHA) in dogs share similar clinical signs including fever, lethargy, icterus, paleness of mucous membranes and splenomegaly. Postmortal findings are similar and, without additional diagnostic methods, an accurate aetiological diagnosis is difficult to achieve. In order to investigate causes of lethal IHA in Croatian dogs, we performed a retrospective study on archived formalin-fixed, paraffin-embedded tissue blocks (FFPEB) from dogs that died due to haemolytic crisis, using microscopic and molecular diagnostic tools to determine the aetiological cause of disease. Molecular analysis was performed on kidney, lung, myocardium and spleen on FFPEB from all dogs. The originally stated aetiological diagnosis of B. canis or leptospirosis was confirmed in only 53% of the dogs. PCR and sequencing revealed that, in addition to the expected pathogens, B. canis and Leptospira interrogans, the presence of previously undiagnosed "new" pathogens causing anaemia including Candidatus Neoehrlichia mikurensis and Anaplasma phagocytophilum. Furthermore, Theileria capreoli was detected for the first time in a dog with postmortal descriptions of lesions. Intensive extravascular hemolysis was noticeable as jaundice of the mucosa, subcutis and fat tissue, green or yellow discoloration of renal parenchyma caused by bilirubin excretion in the renal tubules and bile accumulation within the liver in 90% of the dogs. This work highlights the value of molecular diagnostics to complement traditional ante-mortem and post-mortem diagnostic protocols for the aetiological diagnosis of pathogens associated with IHA.


Assuntos
Anemia Hemolítica/veterinária , Doenças do Cão/diagnóstico , Ehrlichiose/veterinária , Doenças Transmitidas por Carrapatos/veterinária , Anaplasmataceae/genética , Anemia Hemolítica/diagnóstico , Anemia Hemolítica/mortalidade , Animais , Autopsia/veterinária , Doenças do Cão/microbiologia , Doenças do Cão/mortalidade , Doenças do Cão/parasitologia , Cães , Ehrlichiose/diagnóstico , Feminino , Hemólise , Masculino , Técnicas de Diagnóstico Molecular , Inclusão em Parafina , Estudos Retrospectivos , Theileria/genética , Doenças Transmitidas por Carrapatos/diagnóstico
9.
J Prev Med Public Health ; 52(3): 195-199, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-31163955

RESUMO

OBJECTIVES: Jeju Province is well known as the region showing the highest incidence of severe fever with thrombocytopenia syndrome (SFTS) in South Korea. The aim of this study was to evaluate the epidemiological and clinical characteristics of SFTS patients in Jeju Province. METHODS: The primary data for this study were obtained from the Integrated Diseases and Health Control System of the Korea Centers for Disease Control and Prevention (KCDCIS). The selection criteria were confirmed cases of SFTS with a residence listed in Jeju Province at the time of diagnosis, reported to the KCDCIS between July 16, 2014 and November 30, 2018. RESULTS: Of 55 confirmed cases of SFTS, the case fatality rate was 10.9% (95% confidence interval [CI], 4.1 to 22.2). The most common presenting symptoms at diagnosis of severe fever, myalgia, and diarrhea had incidences of 83.6% (95% Cl, 71.2 to 92.2), 45.5% (95% Cl, 32.0 to 59.5), and 40.0% (95% CI, 27.0 to 54.1), respectively. CONCLUSIONS: Compared to SFTS patients nationwide in 2013-2015, the subjects of this study exhibited a lower case fatality rate and had a lower incidence of severe fever, myalgia, and confusion.


Assuntos
Infecções por Bunyaviridae/diagnóstico , Phlebovirus/patogenicidade , Adulto , Idoso , Animais , Infecções por Bunyaviridae/epidemiologia , Feminino , Humanos , Incidência , Masculino , Pessoa de Meia-Idade , República da Coreia/epidemiologia , Doenças Transmitidas por Carrapatos/diagnóstico , Doenças Transmitidas por Carrapatos/epidemiologia , Carrapatos
10.
Med Mal Infect ; 49(5): 335-346, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31155367

RESUMO

The serodiagnosis of Lyme borreliosis is based on a two-tier strategy: a screening test using an immunoenzymatic technique (ELISA), followed if positive by a confirmatory test with a western blot technique for its better specificity. Lyme serology has poor sensitivity (30-40%) for erythema migrans and should not be performed. The seroconversion occurs after approximately 6 weeks, with IgG detection (sensitivity and specificity both>90%). Serological follow-up is not recommended as therapeutic success is defined by clinical criteria only. For neuroborreliosis, it is recommended to simultaneously perform ELISA tests in samples of blood and cerebrospinal fluid to test for intrathecal synthesis of Lyme antibodies. Given the continuum between early localized and disseminated borreliosis, and the efficacy of doxycycline for the treatment of neuroborreliosis, doxycycline is preferred as the first-line regimen of erythema migrans (duration, 14 days; alternative: amoxicillin) and neuroborreliosis (duration, 14 days if early, 21 days if late; alternative: ceftriaxone). Treatment of articular manifestations of Lyme borreliosis is based on doxycycline, ceftriaxone, or amoxicillin for 28 days. Patients with persistent symptoms after appropriate treatment of Lyme borreliosis should not be prescribed repeated or prolonged antibacterial treatment. Some patients present with persistent and pleomorphic symptoms after documented or suspected Lyme borreliosis. Another condition is eventually diagnosed in 80% of them.


Assuntos
Técnicas de Laboratório Clínico , Doença de Lyme , Doenças Transmitidas por Carrapatos , Animais , Técnicas de Laboratório Clínico/métodos , Técnicas de Laboratório Clínico/normas , Diagnóstico Diferencial , Progressão da Doença , França , Humanos , Doença de Lyme/complicações , Doença de Lyme/diagnóstico , Doença de Lyme/patologia , Doença de Lyme/terapia , Guias de Prática Clínica como Assunto , Sociedades Científicas/organização & administração , Sociedades Científicas/normas , Doenças Transmitidas por Carrapatos/complicações , Doenças Transmitidas por Carrapatos/diagnóstico , Doenças Transmitidas por Carrapatos/patologia , Doenças Transmitidas por Carrapatos/terapia
11.
Ticks Tick Borne Dis ; 10(5): 1142-1145, 2019 08.
Artigo em Inglês | MEDLINE | ID: mdl-31213411

RESUMO

BACKGROUND: Tick-borne rickettsioses are infectious diseases caused by obligate intracellular Gram-negative bacteria belonging to the spotted fever groupof Rickettsia. METHODS: We describe an unusual case of SENLAT (Scalp eschar and neck lymphadenopathy after tick bite), caused byRickettsia slovaca, associated with a cellulitis of the face in a 70-year-old woman, and diagnosed using qPCR on a scalp eschar swab. We review the literature regarding cases of SENLAT-associated-cellulitis and case of SENLAT diagnosed by qPCR on scalp eschar swabs. RESULTS: We found only one previous report of SENLAT associated with a cellulitis of the face. It was a nine-year-old French girl diagnosed by seroconversion for Rickettsia sp. Our review of the literature showed that qPCR on eschar swab samples is a less invasive method than performing cutaneous biopsy of the eschar and has good sensitivity and specificity (90% and 100%, respectively). CONCLUSIONS: We report the second case of cellulitis of the face associated with the SENLAT syndrome. Detection of Rickettsia by qPCR on swab sample of the scalp eschar is a simple, noninvasive technique allowing rapid diagnosis and treatment when SENLAT is suspected.


Assuntos
Celulite (Flegmão)/diagnóstico , Linfadenopatia/diagnóstico , Pescoço/patologia , Infecções por Rickettsia/diagnóstico , Rickettsia/isolamento & purificação , Couro Cabeludo/patologia , Idoso , Celulite (Flegmão)/microbiologia , Feminino , França , Humanos , Linfadenopatia/microbiologia , Linfadenopatia/patologia , Infecções por Rickettsia/microbiologia , Infecções por Rickettsia/patologia , Picadas de Carrapatos/microbiologia , Doenças Transmitidas por Carrapatos/diagnóstico , Doenças Transmitidas por Carrapatos/microbiologia , Doenças Transmitidas por Carrapatos/patologia
12.
BMC Infect Dis ; 19(1): 502, 2019 Jun 07.
Artigo em Inglês | MEDLINE | ID: mdl-31174488

RESUMO

BACKGROUND: Francisella tularensis is the causative agent of tularemia in humans and a large number of animal species. Considering recent evidence of the circulation of this bacterium in different parts of Iran, especially in the western provinces, the aim of current study was to determine the tularemia seroprevalence in the human population living in Ilam Province. METHODS: In 2015, 360 serum samples were collected from five groups of people: ranchers (n = 112), farmers (n = 79), butchers and slaughterhouse workers (n = 61), Nature Conservation Officers (n = 34), and referents of medical diagnostic laboratories (n = 74). These samples were tested for the presence of anti- F. tularensis IgG antibodies using the ELISA method. RESULTS: According to the ELISA manufacturer cutoffs, we found that 10 (2.78%) and 9 (2.5%) sera, respectively, were positive or borderline for F. tularensis IgG antibodies. The highest tularemia seroprevalence was observed among farmers (7.59%). CONCLUSIONS: Our results strongly support the circulation of tularemia in Ilam Province. Because no human tularemia case has been reported so far in this province, we recommend specific education programs to increase knowledge of local health care professionals about this important zoonotic disease.


Assuntos
Doenças Transmitidas por Carrapatos/diagnóstico , Tularemia/diagnóstico , Adolescente , Adulto , Animais , Anticorpos Antibacterianos/sangue , Fazendeiros , Feminino , Francisella tularensis/isolamento & purificação , Humanos , Irã (Geográfico)/epidemiologia , Masculino , Pessoa de Meia-Idade , Prevalência , Doenças Transmitidas por Carrapatos/microbiologia , Tularemia/epidemiologia , Adulto Jovem
13.
Med Mal Infect ; 49(5): 318-334, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31097370

RESUMO

Lyme borreliosis is transmitted en France by the tick Ixodes ricinus, endemic in metropolitan France. In the absence of vaccine licensed for use in humans, primary prevention mostly relies on mechanical protection (clothes covering most parts of the body) that may be completed by chemical protection (repulsives). Secondary prevention relies on early detection of ticks after exposure, and mechanical extraction. There is currently no situation in France when prophylactic antibiotics would be recommended. The incidence of Lyme borreliosis in France, estimated through a network of general practitioners (réseau Sentinelles), and nationwide coding system for hospital stays, has not significantly changed between 2009 and 2017, with a mean incidence estimated at 53 cases/100,000 inhabitants/year, leading to 1.3 hospital admission/100,000 inhabitants/year. Other tick-borne diseases are much more seldom in France: tick-borne encephalitis (around 20 cases/year), spotted-fever rickettsiosis (primarily mediterranean spotted fever, around 10 cases/year), tularemia (50-100 cases/year, of which 20% are transmitted by ticks), human granulocytic anaplasmosis (<10 cases/year), and babesiosis (<5 cases/year). The main circumstances of diagnosis for Lyme borreliosis are cutaneous manifestations (primarily erythema migrans, much more rarely borrelial lymphocytoma and atrophic chronic acrodermatitis), neurological (<15% of cases, mostly meningoradiculitis and cranial nerve palsy, especially facial nerve) and rheumatologic (mostly knee monoarthritis, with recurrences). Cardiac and ophtalmologic manifestations are very rarely encountered.


Assuntos
Doença de Lyme , Doenças Transmitidas por Carrapatos , Animais , Babesiose/diagnóstico , Babesiose/epidemiologia , Babesiose/terapia , Encefalite Transmitida por Carrapatos/diagnóstico , Encefalite Transmitida por Carrapatos/epidemiologia , Encefalite Transmitida por Carrapatos/terapia , França/epidemiologia , Humanos , Ixodes/fisiologia , Doença de Lyme/diagnóstico , Doença de Lyme/epidemiologia , Doença de Lyme/prevenção & controle , Guias de Prática Clínica como Assunto , Dermatopatias Bacterianas/diagnóstico , Dermatopatias Bacterianas/epidemiologia , Dermatopatias Bacterianas/terapia , Sociedades Científicas/organização & administração , Sociedades Científicas/normas , Doenças Transmitidas por Carrapatos/diagnóstico , Doenças Transmitidas por Carrapatos/epidemiologia , Doenças Transmitidas por Carrapatos/prevenção & controle
14.
Parasite ; 26: 20, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30943150

RESUMO

Ticks are important vectors of several human and animal pathogens. In this study, we estimated the prevalence of important tick-borne infections in questing ticks from an area in Southwestern France (Hautes-Pyrénées) inhabited by Pyrenean chamois (Rupicapra pyrenaica pyrenaica) experiencing high tick burden. We examined adult and nymph ticks collected by the flag dragging method from 8 to 15 sites in the Pic de Bazès during the years 2009, 2011, 2013 and 2015. PCR assays were conducted on selected ticks for the detection of Borrelia burgdorferi s.l., Babesia spp., Rickettsia spp., spotted fever group (SFG) Rickettsia and Anaplasma phagocytophilum. Randomly selected positive samples were submitted for sequence analysis. A total of 1971 questing ticks were collected including 95 males, 101 females and 1775 nymphs. All collected ticks were identified as Ixodes ricinus. Among them, 696 ticks were selected for pathogen detection and overall prevalence was 8.4% for B. burgdorferi s.l.; 0.4% for Babesia spp.; 6.1% for A. phagocytophilum; 17.6% for Rickettsia spp.; and 8.1% for SFG Rickettsia. Among the sequenced pathogens, we detected in this population of ticks the presence of Babesia sp. EU1 and Rickettsia helvetica, as well as Rickettsia monacensis for the first time in France. The detection of these pathogens in the Pic de Bazès highlights the potential infection risks for visitors to this area and the Pyrenean chamois population.


Assuntos
Ixodes/microbiologia , Ixodes/parasitologia , Rickettsia/isolamento & purificação , Doenças Transmitidas por Carrapatos/diagnóstico , Anaplasma phagocytophilum/genética , Anaplasma phagocytophilum/isolamento & purificação , Animais , Babesia/genética , Babesia/isolamento & purificação , Borrelia burgdorferi/genética , Borrelia burgdorferi/isolamento & purificação , Coinfecção/microbiologia , Coinfecção/parasitologia , DNA Bacteriano/genética , DNA de Protozoário/genética , Feminino , França , Humanos , Masculino , Ninfa , Reação em Cadeia da Polimerase , Saúde Pública , Rickettsia/classificação , Análise de Sequência de DNA , Doenças Transmitidas por Carrapatos/microbiologia
17.
PLoS One ; 14(3): e0213384, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30889229

RESUMO

OBJECTIVE: The aim of this study was to characterize the bacterial microbiome of hard ticks with affinity to bite humans in La Rioja (North of Spain). METHODS: A total of 88 adult ticks (22 Rhipicephalus sanguineus sensu lato, 27 Haemaphysalis punctata, 30 Dermacentor marginatus and 9 Ixodes ricinus) and 120 I. ricinus nymphs (CRETAV collection, La Rioja, Spain), representing the main anthropophilic species in our environment, were subjected to a metagenomic analysis of the V3-V4 region of the 16S rRNA gene using an Illumina MiSeq platform. Data obtained with Greengenes database were refined with BLAST. Four groups of samples were defined, according to the four tick species. RESULTS: Proteobacteria was the predominant phylum observed in all groups. Gammaproteobacteria was the most abundant class, followed by Alphaproteobacteria for R. sanguineus, H. punctata and D. marginatus but the relative abundance of reads for these classes was reversed for I. ricinus. This tick species showed more than 46% reads corresponding to 'not assigned' OTUs (Greengenes), and >97% of them corresponded to 'Candidatus Midichloriaceae' using BLAST. Within Rickettsiales, 'Candidatus Midichloria', Rickettsia, Ehrlichia, 'Candidatus Neoehrlichia' and Wolbachia were detected. I. ricinus was the most alpha-diverse species. Regarding beta-diversity, I. ricinus and H. punctata samples grouped according to their tick species but microbial communities of some R. sanguineus and D. marginatus specimens clustered together. CONCLUSIONS: The metagenomics approach seems useful to discover the spectrum of tick-related bacteria. More studies are needed to identify and differentiate bacterial species, and to improve the knowledge of tick-borne diseases in Spain.


Assuntos
Vetores Aracnídeos/microbiologia , Ixodidae/microbiologia , Metagenoma , Animais , DNA Bacteriano/genética , Dermacentor/microbiologia , Humanos , Ixodes/microbiologia , Metagenômica , Proteobactérias/classificação , Proteobactérias/genética , RNA Ribossômico 16S/genética , Rhipicephalus sanguineus/microbiologia , Espanha , Picadas de Carrapatos/microbiologia , Doenças Transmitidas por Carrapatos/diagnóstico , Doenças Transmitidas por Carrapatos/microbiologia
18.
PLoS Negl Trop Dis ; 13(3): e0007308, 2019 03.
Artigo em Inglês | MEDLINE | ID: mdl-30925154

RESUMO

BACKGROUND: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging viral hemorrhagic fever with a high fatality rate and high frequency of person-to-person transmission and is caused by SFTSV, a tick-borne Phlebovirus. Because SFTS has similar clinical manifestations and epidemic characters (such as spatial and temporal distributions) with hemorrhagic fever with renal syndrome (HFRS) in China, we reason that SFTS patients might be misdiagnosed as HFRS. METHODOLOGY/PRINCIPAL FINDINGS: Acute-phase sera of 128 clinically diagnosed HFRS patients were retrospectively analyzed for Hantavirus IgM antibodies with ELISA. Hantavirus-negative patients' sera were further analyzed for SFTSV IgM antibodies with ELISA. ELISA showed that 73 of 128 (57.0%) of clinically diagnosed HFRS patients were IgM antibody positive to Hantaviruses. Among the 55 Hantavirus-IgM negative patients, four (7.3%) were IgM antibody positive to SFTSV. The results indicated that the four SFTS patients were misdiagnosed as HFRS. The misdiagnosed SFTS patients had clinical manifestations common to HFRS and were unable to be differentiated from HFRS clinically. CONCLUSIONS: Our study showed that SFTS patients could be clinically misdiagnosed as HFRS. The misdiagnosis of SFTS as HFRS causes particular concern because it may increase the risk of death of SFTS patients and person-to-person transmission of SFTSV without proper care for and isolation of SFTS patients.


Assuntos
Hantavirus/imunologia , Febre Hemorrágica com Síndrome Renal/diagnóstico , Febre por Flebótomos/diagnóstico , Phlebovirus/imunologia , Trombocitopenia/diagnóstico , Doenças Transmitidas por Carrapatos/diagnóstico , Adulto , Anticorpos Antivirais/sangue , China/epidemiologia , Erros de Diagnóstico , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Imunoglobulina M/sangue , Masculino , Pessoa de Meia-Idade , Febre por Flebótomos/complicações , Febre por Flebótomos/epidemiologia , Febre por Flebótomos/virologia , Phlebovirus/fisiologia , Estudos Retrospectivos , Trombocitopenia/complicações , Trombocitopenia/epidemiologia , Trombocitopenia/virologia , Doenças Transmitidas por Carrapatos/complicações , Doenças Transmitidas por Carrapatos/epidemiologia , Doenças Transmitidas por Carrapatos/virologia
19.
Parasit Vectors ; 12(1): 116, 2019 Mar 26.
Artigo em Inglês | MEDLINE | ID: mdl-30909941

RESUMO

BACKGROUND: The engagement of companion animal owners into the process of collecting epidemiological data can be facilitated through smartphone applications. In April 2018, the "tekenscanner" (Dutch for tick scanner) app was launched with the aim of engaging pet owners and veterinarians to record ticks removed from their pets and submit these ticks for identification and pathogen testing. Tick-borne pathogens identified in ticks removed from dogs and cats during the first 6 months after the app was launched in the Netherlands are reported. METHODS: The tekenscanner app was used to record the geographical coordinates of ticks removed from dogs or cats onto a map of the Netherlands. A barcode was assigned to each tick for the easy tracking of each submission to our laboratory for taxonomic identification. Thereafter, DNA extracted from the ticks was PCR amplified, subjected to reverse line blot hybridization (RLB) and screened for a broad range of tick-borne pathogens. Results were added to the same app, usually within 2 weeks after the submission of each tick. RESULTS: The app was downloaded 5591 times and resulted in the collection of 1273 georeferenced and barcoded ticks, with a peak submission in May and June of 2018. There were 1005 ticks collected from 406 dogs and 268 ticks collected from 111 cats. Ixodes ricinus was the predominant species (90.0%), with all stages found on dogs as well as on cats. Ixodes hexagonus (7.3%) female and nymphal ticks were also identified on both hosts, whereas adults of Dermacentor reticulatus (2.4%) and Rhipicephalus sanguineus (0.2%) were exclusively found on dogs. Nearly 15% of the ticks recovered from dogs carried one or more pathogens, whereas 13.8% of the ticks removed from cats were infected. Ixodes ricinus collected from dogs contained Borrelia spp. (1.9%), Babesia spp. (0.7%), Anaplasma phagocytophilum (1.3%), "Candidatus Neoehrlichia mikurensis" (2.9%) and Rickettsia helvetica (7.3%). Ixodes ricinus recovered from cats were infected with Borrelia spp. (1.9%), Babesia spp. (0.4%), A. phagocytophilum (1.9%), "Ca. Neoehrlichia mikurensis" (2.6%) and R. helvetica (6.7%). Ixodes hexagonus ticks (n = 93) were not infected. Dermacentor reticulatus ticks, found only in autumn, were infected with Rickettsia raoultii (16 %) and A. phagocytophilum. Three R. sanguineus, on dogs from France and the USA imported into the Netherlands, were all negative. CONCLUSIONS: The tekenscanner app is a versatile tool to use for submission of ticks and facilitated the fast feedback of test results. Community engagement through the app is suitable for identifying hotspots for ticks and tick-borne pathogens and provided an early warning system for exotic ticks invading the Netherlands.


Assuntos
Doenças do Gato/epidemiologia , Doenças do Cão/epidemiologia , Aplicativos Móveis , Animais de Estimação/parasitologia , Smartphone , Infestações por Carrapato/veterinária , Doenças Transmitidas por Carrapatos/epidemiologia , Carrapatos/parasitologia , Animais , Doenças do Gato/diagnóstico , Doenças do Gato/parasitologia , Gatos , Doenças do Cão/diagnóstico , Doenças do Cão/parasitologia , Cães , Monitoramento Epidemiológico/veterinária , Feminino , Masculino , Tipagem Molecular/veterinária , Países Baixos/epidemiologia , Filogeografia , Infestações por Carrapato/epidemiologia , Doenças Transmitidas por Carrapatos/diagnóstico
20.
J Infect Chemother ; 25(6): 480-484, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30824300

RESUMO

Severe fever with thrombocytopenia syndrome (SFTS) is an emerging tick-borne disease caused by a novel bunyavirus. The mechanism underlying disease progression remains unknown, and effective treatment strategy for SFTS is yet to be completely established, making its increasing incidence and subsequent mortality a great concern. Here, we present the autopsy case of a patient with rapidly progressed, fatal SFTS infection. Her viral titer and serum cytokines levels were measured daily and compared with the values of a survivor of the infection. Our findings elucidate the clinical features and pathophysiology of SFTS.


Assuntos
Infecções por Bunyaviridae/diagnóstico , Citocinas/sangue , Phlebovirus/isolamento & purificação , Doenças Transmitidas por Carrapatos/diagnóstico , Carga Viral , Idoso , Infecções por Bunyaviridae/sangue , Infecções por Bunyaviridae/imunologia , Cadáver , Citocinas/imunologia , Evolução Fatal , Feminino , Humanos , Phlebovirus/imunologia , Prognóstico , Doenças Transmitidas por Carrapatos/sangue , Doenças Transmitidas por Carrapatos/imunologia
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