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1.
PLoS One ; 16(3): e0248578, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33765012

RESUMO

The epidemic of coronavirus disease 2019 (COVID-19), caused by a novel Betacoronavirus named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) became a public health emergency worldwide. Few reports indicate that owned pets from households with at least one human resident that was diagnosed with COVID-19 can be infected by SARS-CoV-2. However, the exposure to SARS-CoV-2 of pets from households with no COVID-19 cases or stray animals remains less assessed. Using real-time reverse transcriptase polymerase chain reaction (RT-PCR) and plaque reduction neutralization test (PRNT90), we investigated the infection and previous exposure of dogs and cats to SARS-CoV-2 during the ongoing COVID-19 epidemic in Rio de Janeiro, Brazil. From June to August 2020, 96 animals were sampled, including 49 cats (40 owned and 9 stray) and 47 dogs (42 owned and 5 stray). Regarding owned pets, 75.6% (62/82) belonged to households with no COVID-19 cases. Samples included serum, and rectal and oropharyngeal swabs. All swabs were negative for SARS-CoV-2 RNA, but serum samples of a stray cat and a stray dog presented neutralizing antibodies for SARS-CoV-2, with PRNT90 titer of 80 and 40, respectively. Serological data presented here suggest that not only owned pets from households with COVID19 cases, but also stray animals are being exposed to SARS-CoV-2 during the COVID-19 pandemic.


Assuntos
Anticorpos Neutralizantes/sangue , /imunologia , Animais , /virologia , Doenças do Gato/patologia , Doenças do Gato/virologia , Gatos , Doenças do Cão/patologia , Doenças do Cão/virologia , Cães , Feminino , Masculino , Orofaringe/virologia , RNA Viral/metabolismo , Reto/virologia , /isolamento & purificação
2.
Vet Clin North Am Small Anim Pract ; 51(1): 1-15, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33187619

RESUMO

Esophagitis in cats and dogs is a consequence of increased exposure of the esophageal mucosa to gastroduodenal reflux. Causes can include anesthesia-related reflux, frequent vomiting, or lodged foreign bodies. An exception is eosinophilic esophagitis, an emerging primary inflammatory disease of the esophagus with a presumed allergic etiology. Reflux esophagitis owing to lower esophageal sphincter incompetence is often suspected; a tentative diagnosis can be made by endoscopic assessment, wireless esophageal pH-monitoring, or histologic examination. Because it can be difficult to distinguish diet-responsive upper gastrointestinal disease from esophagitis, response to treatment with gastric acid suppressants is needed to confirm the tentative diagnosis.


Assuntos
Doenças do Gato/diagnóstico , Doenças do Cão/diagnóstico , Esofagite Péptica/veterinária , Animais , Doenças do Gato/patologia , Doenças do Gato/terapia , Gatos , Doenças do Cão/patologia , Doenças do Cão/terapia , Cães , Esofagite Péptica/diagnóstico
3.
Emerg Microbes Infect ; 9(1): 2322-2332, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33028154

RESUMO

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is the cause of Coronavirus Disease 2019 (COVID-19) and responsible for the current pandemic. Recent SARS-CoV-2 susceptibility studies in cats show that the virus can replicate in these companion animals and transmit to other cats. Here, we present an in-depth study of SARS-CoV-2 infection, disease and transmission in domestic cats. Cats were challenged with SARS-CoV-2 via intranasal and oral routes. One day post challenge (DPC), two sentinel cats were introduced. Animals were monitored for clinical signs, clinicopathological abnormalities and viral shedding. Postmortem examinations were performed at 4, 7 and 21 DPC. Viral RNA was not detected in blood but transiently in nasal, oropharyngeal and rectal swabs and bronchoalveolar lavage fluid as well as various tissues. Tracheobronchoadenitis of submucosal glands with the presence of viral RNA and antigen was observed in airways of the infected cats. Serology showed that both, principals and sentinels, developed antibodies to SARS-CoV-2. All animals were clinically asymptomatic during the course of the study and capable of transmitting SARS-CoV-2 to sentinels. The results of this study are critical for understanding the clinical course of SARS-CoV-2 in a naturally susceptible host species, and for risk assessment.


Assuntos
Betacoronavirus/isolamento & purificação , Doenças do Gato/transmissão , Infecções por Coronavirus/transmissão , Infecções por Coronavirus/veterinária , Suscetibilidade a Doenças , Pandemias/veterinária , Pneumonia Viral/transmissão , Pneumonia Viral/veterinária , Animais , Anticorpos Neutralizantes/sangue , Anticorpos Antivirais/sangue , Líquido da Lavagem Broncoalveolar/química , Doenças do Gato/patologia , Doenças do Gato/virologia , Gatos , Linhagem Celular , Chlorocebus aethiops , Infecções por Coronavirus/patologia , Masculino , Pneumonia Viral/patologia , RNA Viral/análise , RNA Viral/isolamento & purificação , Células Vero , Replicação Viral
4.
J Vet Sci ; 21(5): e70, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-33016017

RESUMO

BACKGROUND: Oral neoplasia has been reported to account for 6-7% of all canine cancer and 3% of all feline cancers. To the authors' knowledge the last epidemiologic analysis of general oral cancer in dogs and cats was published in 1976. OBJECTIVES: The goal of this study was to report contemporary demographic information regarding oral tumors in dogs and cats. METHODS: Information was collected from cats or dogs diagnosed with oral neoplasia from the Veterinary Medical Data Base. Medical records representing cases that presented to one of 26 veterinary teaching hospitals from January 1, 1996 through December 31, 2017 were included. RESULTS: A total of 1,810 dogs and 443 cats were identified. A total of 962 cases (53.6%) of canine oral tumors were classified as malignant and 455 cases as benign (25.4%). The majority of feline oral tumors were classified as malignant (257 cases, 58.1%) and only a few benign (11 cases, 2.5%). The incidence of oral tumors was calculated to be 4.9 per 1,000 dogs (0.5%) and 4.9 per 1,000 cats (0.5%). CONCLUSIONS: This incidence of oral tumors is considerably higher than previously reported in both dogs and cats. These results provide valuable information for generation of hypotheses for future investigations of breed-based and pathology-based oral neoplastic studies.


Assuntos
Doenças do Gato/epidemiologia , Doenças do Cão/epidemiologia , Neoplasias Bucais/veterinária , Animais , Doenças do Gato/patologia , Gatos , Doenças do Cão/patologia , Cães , Feminino , Hospitais de Ensino , Incidência , Masculino , Neoplasias Bucais/epidemiologia , Neoplasias Bucais/patologia , Estados Unidos/epidemiologia
5.
PLoS One ; 15(10): e0239991, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33091006

RESUMO

Shedding of DNA of pathogenic Leptospira spp. has been documented in naturally infected cats in several countries, but urinary shedding of infectious Leptospira spp. has only recently been proven. The climate in Southern Chile is temperate rainy with high annual precipitations which represents ideal preconditions for survival of Leptospira spp., especially during spring and summer. The aims of this study were to investigate shedding of pathogenic Leptospira spp. in outdoor cats in Southern Chile, to perform molecular characterization of isolates growing in culture, and to assess potential risk factors associated with shedding. Urine samples of 231 outdoor cats from rural and urban areas in southern Chile were collected. Urine samples were investigated for pathogenic Leptospira spp. by 4 techniques: qPCR targeting the lipL32 gene, immunomagnetic separation (IMS)-coupled qPCR (IMS-qPCR), direct culture and IMS-coupled culture. Positive urine cultures were additionally confirmed by PCR. Multilocus sequence typing (MLST) was used to molecularly characterize isolates obtained from positive cultures. Overall, 36 urine samples (15.6%, 95% confidence interval (CI) 11.4-20.9) showed positive results. Eighteen (7.8%, 95% CI 4.9-12.1), 30 (13%, 95% CI 9.2-18), 3 (1.3%, 0.3-3.9) and 4 cats (1.7%; 95% CI 0.5-4.5) were positive in qPCR, IMS-qPCR, conventional culture, and IMS-coupled culture, respectively. MLST results of 7 culture-positive cats revealed sequences that could be assigned to sequence type 17 (6 cats) and sequence type 27 (1 cat) corresponding to L. interrogans (Pathogenic Leptospira Subgroup 1). Shedding of pathogenic Leptospira spp. by cats might be an underestimated source of infection for other species including humans. The present study is the first one reporting growth of leptospires from feline urine in culture in naturally infected cats in South-America and characterisation of culture-derived isolates. So far, very few cases of successful attempts to culture leptospires from naturally infected cats are described worldwide.


Assuntos
Derrame de Bactérias/fisiologia , Doenças do Gato/patologia , Leptospira/patogenicidade , Leptospirose/patologia , Animais , Proteínas da Membrana Bacteriana Externa/genética , Proteínas da Membrana Bacteriana Externa/isolamento & purificação , Doenças do Gato/microbiologia , Doenças do Gato/transmissão , Gatos , DNA Bacteriano/metabolismo , Feminino , Leptospira/genética , Leptospira/isolamento & purificação , Leptospirose/microbiologia , Leptospirose/transmissão , Leptospirose/veterinária , Lipoproteínas/genética , Lipoproteínas/isolamento & purificação , Masculino , Tipagem de Sequências Multilocus , Reação em Cadeia da Polimerase em Tempo Real , Fatores de Risco , Urina/microbiologia
6.
Parasitol Res ; 119(10): 3443-3450, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32813040

RESUMO

Feline lungworms infect the respiratory tract of wild and domestic cats, causing infection often associated with clinical disease. Until recently, Aelurostrongylus abstrusus has been considered the most relevant species of lungworm, while Troglostrongylus brevior was considered of less significance. Fecal samples of feral cats from Jerusalem, Israel, collected over a year, were examined for first stage lungworm larvae (L1) using the Baermann method. Positive samples were morphologically identified, and their species identity was molecularly confirmed. Forty of 400 (10.0%) cats were lungworm-positive, of which 38/40 (95.0%) shed Troglostrongylus brevior and 6/40 (15.0%) shed Aelurostrongylus abstrusus. Four cats (10.0%) had mixed infections with both lungworm species. L1 shedding was associated with clinical respiratory signs in 11 (19.0%) T. brevior shedding cats of a total of 58 cats manifesting respiratory signs, while 23/342 (6.7%) cats without respiratory signs were L1-positive (p = 0.006). Non-respiratory clinical signs were also found to be more prevalent in L1 shedders (p = 0.012). A young kitten ≤ 4 weeks of age shed T. brevior L1 larvae. DNA sequences of both lungworm species using the ribosomal internal transcribed spacer 2 (ITS2) locus were > 99% similar to other sequences deposited in GenBank, suggesting that T. brevior and A. abstrusus ITS2 sequences are both highly conserved. In conclusion, L1 shedding in feral cats from Jerusalem were mostly caused by T. brevior with only a small proportion involving A. abstrusus, different from many studies from other geographical regions.


Assuntos
Doenças do Gato/parasitologia , Metastrongyloidea/isolamento & purificação , Infecções por Strongylida/veterinária , Animais , Animais Selvagens , Doenças do Gato/epidemiologia , Doenças do Gato/patologia , Gatos , Fezes/parasitologia , Israel/epidemiologia , Larva/classificação , Larva/genética , Larva/crescimento & desenvolvimento , Metastrongyloidea/classificação , Metastrongyloidea/genética , Metastrongyloidea/crescimento & desenvolvimento , Prevalência , Infecções por Strongylida/epidemiologia , Infecções por Strongylida/parasitologia , Infecções por Strongylida/patologia
7.
Rev Bras Parasitol Vet ; 29(2): e023519, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32609248

RESUMO

An adult male puma (Puma concolor), hit by a car in an urban area, died three days later despite the therapeutic support provided. At necropsy, multiple firm nodules were identified in the gastric mucosa. The nodules were coated by an intact mucosa with a central opening from which reddish and cylindrical nematodes protruded into the lumen. Twenty-seven nematodes were retrieved for morphological and morphometric evaluations. During histopathological examination of the gastric tissue, the adult nematodes appear in longitudinal and transverse sections, surrounded by thick bands of collagen, interspersed with mixed inflammatory infiltrates. The nematodes had an eosinophilic cuticle with caudal serrated projections (bulbar type), coelomyarian musculature, pseudocoelom, and females with uterus containing numerous larvated eggs, characteristics consistent with the Cylicospirura genus. Morphologically, female nematodes had six large tricuspid teeth in the oral cavity and the vulva had an opening anterior to the esophagus-intestinal junction. Male nematodes had five pairs of small papillae near the tip of the tail. These findings were consistent with Cylicospirura felineus. This parasite should be included in the differential diagnosis of nodular gastric wall lesions in wild felids.


Assuntos
Gastrite , Nematoides , Puma , Animais , Doenças do Gato/parasitologia , Doenças do Gato/patologia , Gatos , Feminino , Gastrite/parasitologia , Gastrite/veterinária , Masculino , Nematoides/fisiologia , Infecções por Nematoides/parasitologia , Infecções por Nematoides/patologia , Infecções por Nematoides/veterinária , Puma/parasitologia
8.
Exp Hematol ; 86: 43-52.e1, 2020 06.
Artigo em Inglês | MEDLINE | ID: mdl-32417302

RESUMO

Whole transferrin receptor (TfR) is present in reticulocyte exosomes. Soluble transferrin receptor (sTfR) is cleaved from whole TfR in human plasma, with the remnant cytoplasmic domain (cTfR) remaining membrane associated. In humans, sTfR is a biomarker that can detect iron deficiency in the presence of inflammatory disease. This condition is still a diagnostic dilemma in veterinary species. We aimed to (1) confirm the presence of exosomes and exosome-associated TfR in the serum of dogs, cats, and horses; and (2) to assess and compare the proportion of cTfR to total (cTfR + whole) in exosomal membranes of healthy and diseased dogs and cats and in healthy horses to indirectly predict their anticipated levels of circulating sTfR. We used discarded serum and whole blood samples from canine and feline patients, separated into healthy and diseased groups based on the health status of each patient, and healthy equine participants from a previous study. Ultracentrifugation, followed in some experiments by OptiPrep discontinuous density gradient fractionation, was used to isolate exosomes. Exosomes and associated TfR were identified using TEM and Western blot for TfR, respectively. Densitometry tracings of Western blots of serum exosomes were used to measure the proportion of cTfR to total TfR. Extracellular vesicles compatible with exosomes were successfully isolated and expressed TfR. The proportion of cTfR in dogs was greater than 50%, indicating that a majority of the whole TfR was cleaved to produce sTfR (and remnant cTfR). There was significant interindividual variation and no significant difference between healthy and diseased animals. The proportion of cTfR in cats was very low at 11%, indicating that very little sTfR was likely produced. There was a small yet significant difference between healthy and diseased cats. Healthy horses do not appear to cleave exosome-associated TfR. Diagnosis of iron deficiency in the presence of inflammatory disease remains a challenge in veterinary medicine. Our results indicate that TfR is poorly or unpredictably cleaved in veterinary species, revealing that there are species differences in exosomal TfR handling. These data suggest that development of an assay for the detection and quantification of sTfR in the species investigated may not be warranted.


Assuntos
Doenças do Gato/sangue , Doenças do Cão/sangue , Exossomos/metabolismo , Doenças dos Cavalos/sangue , Receptores da Transferrina/sangue , Animais , Doenças do Gato/patologia , Gatos , Doenças do Cão/patologia , Cães , Exossomos/patologia , Doenças dos Cavalos/patologia , Cavalos
9.
Vet Clin North Am Small Anim Pract ; 50(4): 823-882, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32387302

RESUMO

Facial dermatitis in cats can be caused by a broad range of infectious, allergic, immune-mediated and neoplastic disorders with very different treatments and prognoses. Baseline dermatologic diagnostics (skin scrapings for mites, cytology for infection and to characterize inflammatory infiltrate, and dermatophyte culture) are required, as well as possible further diagnostics, including therapeutic trials for parasites and feeding a hypoallergenic diet, bacterial culture, and skin biopsies for histopathology in order to achieve a diagnosis. Clinical presentations of diseases affecting different parts of the feline face are presented and discussed.


Assuntos
Doenças do Gato/diagnóstico , Dermatite/veterinária , Animais , Doenças do Gato/patologia , Gatos , Dermatite/diagnóstico , Orelha , Face , Nariz
11.
J Vet Diagn Invest ; 32(3): 454-457, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32274976

RESUMO

An 8-y-old castrated male, outdoor European shorthair cat was presented with a history of hindlimb weakness and paralysis. Disease progression was continuous from the onset; deep algesia disappeared at the final stage. Radiography of the vertebral column was unremarkable; along with patient history and physical examination results, magnetic resonance imaging suggested inflammatory lesions in the spinal cord, although neoplasia could not be ruled out. Feline leukemia virus (FeLV) positivity was confirmed by a serum ELISA prior to euthanasia. Upon postmortem examination, hemorrhages were present in the spinal cord at the level of vertebrae T7-8. Histologic and immunohistochemical analysis revealed primary diffuse large B-cell lymphoma of the spinal cord with multifocal myelomalacia and hemorrhages. To determine the presence of a pathogen within the lesion, we developed a novel in situ hybridization protocol for FeLV (RNAscope). The reaction revealed large amounts of FeLV viral RNA in the tumor cells.


Assuntos
Doenças do Gato/virologia , Hibridização In Situ/veterinária , Vírus da Leucemia Felina/genética , Linfoma de Células B/veterinária , Infecções por Retroviridae/veterinária , Infecções Tumorais por Vírus/veterinária , Animais , Doenças do Gato/patologia , Gatos , Vírus da Leucemia Felina/fisiologia , Linfoma de Células B/patologia , Linfoma de Células B/virologia , Masculino , RNA Viral/análise , RNA Viral/isolamento & purificação , Infecções por Retroviridae/patologia , Infecções por Retroviridae/virologia , Infecções Tumorais por Vírus/patologia , Infecções Tumorais por Vírus/virologia
12.
Top Companion Anim Med ; 38: 100369, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32115079

RESUMO

The objective of this study was to collect preliminary data about tooth resorption (TR) from cats treated at the Odontology Service (September 2016-June 2018), part of a University Veterinary Hospital in Spain, with specific emphasis on TR distribution per tooth. Diagnosis was based on visual/tactile inspection and intraoral dental radiographs. This cross-sectional study was carried out on 59 adult cats (27 females, 32 males). TR occurred in 39/59 cats (66.1%; 95% CI: 54.0%-78.2%). The median number of lesioned teeth per TR-affected animal was 3. A highly significant but weak correlation was found for age and number of TR-affected teeth per individual (Spearman´s correlation ρ = 0.381, P = .003, power = 0.853; N = 59). No TR cases were detected in incisors (0/708, 0%) but TR occurred in canines (21/236, 8.9%; 95% CI: 5.4%-12.4%), premolars (78/590, 13.2%; 95% CI: 10.5%-15.9%), and molars (33/236, 14%; 95% CI: 9.7%-18.3%). A significant age influence on TR was found. The greatest TR occurrence corresponded to 307 (21/59; 35.6%; 95% CI: 23.4%-47.8%) followed by 409 (17/59; 28.8%; 95% CI: 17.2%-40.3%), 407 (16/59; 27.1%; 95% CI: 15.7%-38.4%), and 309 (16/59; 27.1%; 95% CI: 15.7%-38.4%). These teeth would be considered as TR-sentinels in the studied population. These findings are relevant for veterinarians working in dental clinics, where the TR prevalence may be high especially in older cats.


Assuntos
Doenças do Gato/patologia , Reabsorção de Dente/veterinária , Fatores Etários , Animais , Doenças do Gato/diagnóstico por imagem , Gatos , Estudos Transversais , Feminino , Masculino , Radiografia Dentária/veterinária , Espanha , Reabsorção de Dente/diagnóstico por imagem , Reabsorção de Dente/patologia
13.
J Vet Emerg Crit Care (San Antonio) ; 30(3): 325-330, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32141165

RESUMO

OBJECTIVE: To document a case of transient AB blood type indicated by immunochromatography in a type B cat following administration of an incompatible type A transfusion. CASE SUMMARY: A 7-month-old neutered male domestic longhair cat was evaluted for anemia, pigmenturia, and intravascular hemolysis 1 day after receiving a feline whole blood transfusion. Neither blood donor nor patient had been blood-typed or crossmatched. The cat presented in shock with a severe non-regenerative anemia, hyperlactatemia, hyperbilirubinemia, hemoglobinemia, hemoglobinuria, and a positive saline slide agglutination test. Immunochromatographic blood typing tests initially indicated the cat had type AB blood, but crossmatch tests with blood from type A and type B donors suggested that the cat was type B. The cat was transfused with type B packed red blood cells without apparent complications and clinically improved. The cat's blood type reverted to type B once all the previously transfused type A cells were cleared from circulation. Furthermore, the original donor was subsequently identified as a Siamese cat and confirmed to have type A blood. While the cause of the original anemia remained unknown, the cat completely recovered and regained a normal hematocrit. NEW OR UNIQUE INFORMATION PROVIDED: This is the first documented report of transient AB blood type diagnosed using immunochromatography after a transfusion mismatch and shows the utility of crossmatching or back-typing to identify the cat's correct blood type during the hemolytic transfusion reaction.


Assuntos
Anemia/veterinária , Incompatibilidade de Grupos Sanguíneos/veterinária , Tipagem e Reações Cruzadas Sanguíneas , Transfusão de Sangue/veterinária , Doenças do Gato/etiologia , Reação Transfusional/veterinária , Animais , Doadores de Sangue , Antígenos de Grupos Sanguíneos , Doenças do Gato/patologia , Gatos , Hemólise , Masculino , Complicações Pós-Operatórias , Choque
14.
Open Vet J ; 9(4): 331-334, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-32042655

RESUMO

Background: Although bone tumors are common pathologies in companion animals, limited reports describe nasal osteosarcoma (OSA) in cats. Case description: A case of nasal OSA in a local Libyan cat was admitted to the Veterinary Teaching Hospital at the Faculty of Veterinary Medicine, University of Tripoli-Libya, with nasal swelling and discharges and facial deformity. The radiological findings revealed nasal osteolysis with the absence of evidence of lung metastasis. In addition, fungal growth was not identified in microbiological culture. Furthermore, the pathological examination has grossly revealed a destructed nasal bone due to the presence of a tumor mass, with a mucohemorrhagic nasal discharge and absence of metastasis. OSA was confirmed histopathologically. Conclusion: This report presents the clinical, radiological, and pathological findings of a primary nasal OSA in a Libyan cat with no tumor metastasis to other body organs.


Assuntos
Neoplasias Ósseas/veterinária , Doenças do Gato/patologia , Neoplasias Nasais/veterinária , Osteossarcoma/veterinária , Animais , Neoplasias Ósseas/diagnóstico por imagem , Neoplasias Ósseas/patologia , Doenças do Gato/diagnóstico por imagem , Gatos , Feminino , Neoplasias Nasais/diagnóstico por imagem , Neoplasias Nasais/patologia , Osteossarcoma/diagnóstico por imagem , Osteossarcoma/patologia
15.
BMC Vet Res ; 16(1): 26, 2020 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-32000761

RESUMO

BACKGROUND: Hypertrophic cardiomyopathy is the most common cardiovascular cause of death in cats. Although the majority of cats remain asymptomatic, some may develop signs of chronic heart failure due to diastolic failure, arterial thromboembolism (ATE) or sudden cardiac death. Therefore, it is crucial to identify individuals that are in high risk of developing cardiac complications before the onset of life-threatening signs. Oxidative stress is the imbalance between the production and neutralisation of reactive oxygen species. Uncontrolled reactive oxygen species overproduction leads to protein and lipid peroxidation and damages the DNA strands, injuring the cells and leading to their death. The aim of the study was to evaluate the oxidative state in cats with hypertrophic cardiomyopathy and healthy controls. RESULTS: In total, 30 cats divided into three groups were assessed: animals with clinically evident hypertrophic cardiomyopathy (HCM; n = 8), subclinical hypertrophic cardiomyopathy (SUB-HCM; n = 11) and healthy controls (n = 11). The activity of superoxide dismutase was statistically significantly lower in animals with symptomatic and asymptomatic hypertrophic cardiomyopathy (HCM 0.99 ± 0.35 U/mL; SUB-HCM 1.39 ± 0.4 U/mL) compared to healthy cats (2.07 ± 0.76 U/mL, p < 0.01). The activity of catalase was significantly lower in the SUB-HCM group (19.4 ± 4.2 nmol/min/mL) compared to the HCM (23.6 ± 5.9 nmol/min/mL) and the control (30 ± 7.5 nmol/min/mL, p < 0.01) group. The activity of glutathione peroxidase was 4196 ± 353 nmol/min/mL in the HCM group, 4331 ± 451 nmol/min/mL in the SUB-HCM group and 4037 ± 341 nmol/min/mL in the control group and did not differ significantly between groups. The total antioxidant capacity of plasma was 602 ± 65.5 copper reducing equivalents (CRE) in the HCM group, 605.9 ± 39.9 CRE in the SUB-HCM group and 629 ± 77.5 CRE in the healthy cats and did not differ significantly between the groups. CONCLUSIONS: Activities of superoxide dismutase and catalase differed in cats with hypertrophic cardiomyopathy, however the activity of the latter was only significantly lower in asymptomatic stage of the disease. The potentially beneficial effect of antioxidative substances on the disease progression in the asymptomatic and symptomatic stage of this disease should also be examined.


Assuntos
Antioxidantes/análise , Cardiomiopatia Hipertrófica/veterinária , Doenças do Gato/patologia , Estresse Oxidativo , Animais , Biomarcadores/sangue , Cardiomiopatia Hipertrófica/enzimologia , Doenças do Gato/enzimologia , Catalase/sangue , Gatos , Estudos Transversais , Ecocardiografia/veterinária , Feminino , Masculino , Projetos Piloto , Superóxido Dismutase/sangue
16.
J Comp Pathol ; 174: 34-38, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31955801

RESUMO

A 5-year-old neutered male domestic shorthaired cat was presented with neurological signs. A primary cardiac lymphoma (PCL) with metastasis to the meninges of the brain was diagnosed histologically. Immunohistochemistry confirmed a B-cell lymphoma. Reports of PCL in cats are scarce. The current case describes a primary cardiac B-cell lymphoma in a cat confirmed by histopathology and immunohistochemistry, and underscores the significance of histopathology for definitive diagnosis.


Assuntos
Doenças do Gato/patologia , Neoplasias Cardíacas/veterinária , Linfoma de Células B/veterinária , Animais , Gatos , Masculino
17.
Sci Rep ; 10(1): 1003, 2020 01 22.
Artigo em Inglês | MEDLINE | ID: mdl-31969654

RESUMO

Feline mammary carcinomas (FMCs) are highly malignant. As the disease-free survival (DFS) and overall survival (OS) are short, prognostication is crucial. Copy-number variations (CNVs) analysis by next-generation sequencing serves to identify critical cancer-related genomic regions. Thirty-three female cats with FMCs were followed during two years after surgery. Tumours represented tubulopapillary and solid carcinomas encompassing six molecular subtypes. Regardless of the histopathological diagnosis, molecular subtypes showed important differences in survival. Luminal A tumours exhibited the highest DFS (p = 0.002) and cancer-specific OS (p = 0.001), and the lowest amount of CNVs (p = 0.0001). In contrast, basal-like triple-negative FMCs had the worst outcome (DFS, p < 0.0001; and OS, p < 0.00001) and were the most aberrant (p = 0.05). In the multivariate analysis, copy-number losses (CNLs) in chromosome B1 (1-23 Mb) harbouring several tumour-repressors (e.g. CSMD1, MTUS1, MSR1, DBC2, and TUSC3) negatively influenced DFS. Whereas, copy-number gains (CNGs) in B4 (1-29 Mb) and F2 (64-82.3 Mb) comprising epithelial to mesenchymal transition genes and metastasis-promoting transcription factors (e.g. GATA3, VIM, ZEB1, and MYC) negatively influenced DFS and cancer-specific OS. These data evidence an association between specific CNVs in chromosomes B1, B4 and F2, and poor prognosis in FMCs.


Assuntos
Doenças do Gato/genética , Variações do Número de Cópias de DNA/genética , Neoplasias Mamárias Animais/genética , Animais , Doenças do Gato/mortalidade , Doenças do Gato/patologia , Gatos , Feminino , Sequenciamento de Nucleotídeos em Larga Escala/veterinária , Neoplasias Mamárias Animais/mortalidade , Neoplasias Mamárias Animais/patologia , Análise de Sobrevida
19.
J Vet Cardiol ; 27: 23-33, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31931390

RESUMO

INTRODUCTION/OBJECTIVES: Recognition of congestive heart failure (CHF) in dyspnoeic cats is crucial for correct intervention. The pulmonary vein (PV) to pulmonary artery (PA) ratio (PV/PA) has been proposed as an index that might help discriminate dogs suffering from CHF but has never been studied in cats. We sought to determine reference intervals for various PV and PA variables in healthy cats. We then examined these variables in cats with subclinical and clinical cardiomyopathies to determine their diagnostic utility in identifying CHF. ANIMALS, MATERIALS AND METHODS: We took a sample of 114 cats: 51 healthy cats, 32 subclinical cardiomyopathy affected cats and 31 cardiomyopathic cats with CHF. PV and PA were measured at the minimal and maximal diameters using M-mode images obtained from a modified right parasternal long axis view. The aorta (Ao) and left atrium were measured using two-dimensional imaging employing the right parasternal short axis view. RESULTS: median PVmin/PAmin value in healthy cats was approximately 0.51 and the PVmax/PAmax value was 0.67. The median distensibility value of the vessels was 23% for ΔPA and 41% for ΔPV. Cats with CHF had higher PVmin/PAmin, PVmax/PAmax, PVmin/Ao, PVmax/Ao values and a smaller ΔPV value compared to subclinical and healthy cats (p < 0.0001). When evaluating the diagnostic performance of these variables (in cardiomyopathic cats), PVmin/PAmin and PVmin/Ao values had higher accuracy compared to the LA:Ao value when identifying cats with CHF. CONCLUSIONS: Our study provides reference values for PV and PA variables in cats. Moreover, PV/PA variables were better factors than LA:Ao for discriminating cardiomyopathic cats with and without CHF.


Assuntos
Cardiomiopatias/veterinária , Doenças do Gato/diagnóstico por imagem , Artéria Pulmonar/diagnóstico por imagem , Veias Pulmonares/diagnóstico por imagem , Animais , Cardiomiopatias/diagnóstico por imagem , Doenças do Gato/patologia , Gatos , Dispneia/veterinária , Ecocardiografia/veterinária , Feminino , Insuficiência Cardíaca/diagnóstico por imagem , Insuficiência Cardíaca/veterinária , Masculino
20.
J Am Anim Hosp Assoc ; 56(2): e56202, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31961219

RESUMO

A 12 yr old spayed female domestic shorthair with a history of lethargy, anorexia, and a pendulous abdomen was referred after a cranial abdominal mass was palpated on physical examination. Thoracic radiographs and an abdominal ultrasound revealed a mass associated with the kidney and moderate hemoperitoneum. Exploratory laparotomy revealed abdominal hemorrhage originating from a right renal mass that was adhered to the caudal vena cava. Following a right nephrectomy, histopathology diagnosed the mass as a perirenal/renal myxosarcoma. Based upon thoracic radiographs and abdominal ultrasound, the patient remains disease free at 14 mo postoperatively.


Assuntos
Doenças do Gato/patologia , Neoplasias Renais/veterinária , Mixossarcoma/veterinária , Animais , Doenças do Gato/cirurgia , Gatos , Feminino , Neoplasias Renais/patologia , Neoplasias Renais/cirurgia , Mixossarcoma/patologia , Mixossarcoma/cirurgia
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