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1.
Rev Bras Parasitol Vet ; 29(1): e018319, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32049149

RESUMO

Scuticociliatosis, caused by an opportunistic ciliate protozoan, is responsible for significant economic losses in marine ornamental fish. This study reports the occurrence of Uronema spp., parasitizing ten species of marine reef fish at an ornamental fish wholesaler: Blue green damselfish (Chromis viridis), Vanderbilt's Chromis (Chromis vanderbilti), Pennant coralfish (Heniochus acuminatus), Threespot angelfish (Apolemichthys trimaculatus), Goldspotted angelfish (Apolemichthys xanthopunctatus), Sea goldie (Pseudanthias squamipinnis), Orchid dottyback (Pseudochromis fridmani), Threadfin butterflyfish (Chaetodon auriga), Vagabond butterflyfish (Chaetodon vagabundus), and Bluecheek butterflyfish (Chaetodon semilarvatus). Diseased fish showed disorders such as hemorrhages and ulcerative lesions on the body surface. Histopathological analysis of the muscle, liver, gut, kidney, spleen, gills, and stomach revealed hemorrhages and degeneration of muscle fiber, vacuolar degeneration of hepatocyte, inflammatory process and granuloma in the liver, atrophy of intestinal villi, inflammatory process and granuloma in the kidney, melanomacrophage centers, as well as inflammatory process in the spleen, epithelial cells hyperplasia and granuloma formation in the gills, and vacuolar degeneration and eosinophils in the stomach. Due to the severity of the disease, it is necessary to implement biosecurity measures with rapid and accurate diagnosis to minimize the risk of economic losses caused by Uronema spp.


Assuntos
Infecções por Cilióforos/veterinária , Cilióforos/classificação , Doenças dos Peixes/parasitologia , Perciformes/parasitologia , Animais , Brasil , Infecções por Cilióforos/parasitologia , Infecções por Cilióforos/patologia , Doenças dos Peixes/patologia
3.
J Fish Dis ; 43(1): 91-100, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31724204

RESUMO

In 2012, brown bullhead (Ameiurus nebulosus) with large, raised, black growths were first reported from multiple areas within the Vermont portion of Lake Memphremagog. Subsequent surveys conducted from 2014 to 2017 at two sites within the lake indicated a prevalence of 30% in adult brown bullhead 200 mm and above total length. These lesions ranged from slightly raised smooth black areas to large nodular areas on the body surface and fins and within the oral cavity. Microscopically, these lesions were determined to be malignant melanoma with invasion into surrounding hypodermis, skeletal muscle and bone as well as metastases to gill, ovary and intestine. Liver neoplasms were also observed in 8% of the bullhead collected from Lake Memphremagog in 2015. Neither skin nor liver neoplasms were noted in Ticklenaked Pond, a site used for comparison.


Assuntos
Doenças dos Peixes/epidemiologia , Ictaluridae , Melanoma/veterinária , Animais , Feminino , Doenças dos Peixes/patologia , Lagos , Neoplasias Hepáticas/epidemiologia , Neoplasias Hepáticas/patologia , Neoplasias Hepáticas/veterinária , Masculino , Melanoma/epidemiologia , Melanoma/patologia , Prevalência , Quebeque/epidemiologia , Dermatopatias/epidemiologia , Dermatopatias/patologia , Dermatopatias/veterinária , Vermont/epidemiologia
4.
J Fish Dis ; 43(1): 69-80, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31642063

RESUMO

Freshwater mussels of the order Unionida encyst into the fish mucosa to metamorphose and complete their life cycle, causing a parasitic disease known as glochidiosis. This parasitic stage represents a bottleneck for the survival of naiads, particularly for critically endangered species as Margaritifera margaritifera; however, little is known about the events occurring during this critical stage. Therefore, this study aimed to histologically characterize the development of M. margaritifera glochidiosis in Atlantic salmon to get insight into the pathogenesis of this interaction. Fish exposed to glochidia were sampled during the first 44 days post-exposure, and organs were observed by stereomicroscopy and light microscopy. Glochidia attached to the gills by pinching the lamellar epithelium, whereupon an acute proliferative branchitis engulfed most of the larvae. However, during the first 14 days, a severe detachment of unviable glochidia occurred, associated with the presence of pleomorphic inflammatory infiltrate and epithelial degeneration. In the cases where larvae remained attached, a chronification of the lesions with none to scarce inflammation was observed. These results provide key information to better understand the complex host-parasite interaction during the early stages of glochidiosis and provide valuable information to optimize artificial rearing of naiads in conservation of threatened freshwater mussel populations.


Assuntos
Bivalves/fisiologia , Doenças dos Peixes/patologia , Brânquias/parasitologia , Salmo salar , Animais , Bivalves/crescimento & desenvolvimento , Doenças dos Peixes/parasitologia
6.
Parasitol Res ; 118(12): 3241-3252, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31728722

RESUMO

Myxobolus neurofontinalis n. sp. infects the brain and medulla oblongata of brook trout (Salvelinus fontinalis [Mitchill, 1814]) in the New River, western NC. It is the first species of Myxobolus described from the brook trout and resembles another congener (Myxobolus arcticus Pugachev and Khokhlov, 1979) that infects nerve tissue of chars (Salvelinus spp.). The new species differs from M. arcticus and all congeners by myxospore dimensions and by having a mucous envelope and distinctive sutural markings. A phylogenetic analysis of the small subunit rDNA (18S) suggests that the new species shares a recent common ancestor with some isolates identified as M. arcticus and that the new species and its close relatives (except Myxobolus insidiosus Wyatt and Pratt, 1973) comprise a clade of salmonid nerve-infecting myxobolids. The phylogenetic analysis indicates that several isolates of "M. arcticus" (sensu lato) in GenBank are misidentified and distantly related to other isolates taken from the type host (Oncorhynchus nerka [Walbaum, 1792]) and from nearby the type locality (Kamchatka Peninsula, Russia). Serial histological sections of infected brook trout confirmed that myxospores of the new species are intercellular and infect nerve cord and medulla oblongata only. A single infected brook trout showed an inflammatory response characterized by focal lymphocytic infiltrates and eosinophilic granulocytes; however, the remaining 4 brook trout lacked evidence of a histopathological change or demonstrable host response. These results do not support the notion that this infection is pathogenic among brook trout.


Assuntos
Doenças dos Peixes/parasitologia , Bulbo/parasitologia , Myxobolus/classificação , Tecido Nervoso/parasitologia , Truta/parasitologia , Animais , Região dos Apalaches , Doenças dos Peixes/patologia , Myxobolus/genética , Filogenia , RNA Ribossômico 18S/genética , Especificidade da Espécie
7.
Syst Parasitol ; 96(9): 767-776, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31721045

RESUMO

A Henneguya sp., morphologically resembling Henneguya nyongensis Fomena & Bouix, 1996, was isolated from the gills of Peter's elephantnose fish, Gnathonemus petersii Günther, imported from Nigeria. Plasmodia were located between lamellae and within the gill epithelium, often leading to lamellar fusion. Although slightly smaller, the myxospores from these fish were morphologically consistent with H. nyongensis. In valvular view, spores are elongate, pyriform with a rounded posterior and tapering caudal processes. Myxospore bodies are 9.6-12.3 (mean 11.2) µm long and 4.0-4.7 (mean 4.3) µm wide. Polar capsules are pyriform, elongate, 4.5-5.2 (4.7) µm long and 1.3-1.6 (1.4) µm wide, with a characteristic neck-like structure at the apical end. Sequence generated for the 18S small subunit rRNA gene did not directly match any sequences available on GenBank, but demonstrated 91% nucleotide similarity to an unpublished Henneguya sp. infecting Mormyrus kannume Forsskål. Herein, the description of H. nyongensis is supplemented with new data on histopathology, molecular characterisation, and expanded host and geographical range.


Assuntos
Cnidários/classificação , Peixe Elétrico/parasitologia , Doenças dos Peixes/parasitologia , Doenças Parasitárias em Animais/parasitologia , Animais , Cnidários/anatomia & histologia , Cnidários/genética , Doenças dos Peixes/patologia , Brânquias/parasitologia , Brânquias/patologia , Nigéria , Doenças Parasitárias em Animais/patologia , RNA Ribossômico 18S/genética , Especificidade da Espécie
8.
J Fish Dis ; 42(12): 1667-1676, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31612537

RESUMO

In recent years, the use of cleaner fish for biological control of sea lice has increased considerably. Along with this, a number of infectious diseases have emerged. The aim of this study was to investigate the susceptibility of lumpfish (Cyclopterus lumpus) to Betanodavirus since it was detected in asymptomatic wild wrasses in Norway and Sweden. Three betanodaviruses were used to challenge lumpfish: one RGNNV genotype and two BFNNV genotypes. Fish were injected and monitored for 4 weeks. Brain samples from clinically affected specimens, from weekly randomly selected fish and survivors were subjected to molecular testing, viral isolation, histopathology and immunohistochemistry. Reduced survival was observed but was attributed to tail-biting behaviour, since no nervous signs were observed throughout the study. Betanodavirus RNA was detected in all samples, additionally suggesting an active replication of the virus in the brain. Viral isolation confirmed molecular biology results and revealed a high viral titre in BFNNV-infected groups associated with typical lesions in brains and eyes of survivor fish. We concluded that lumpfish are susceptible to Betanodavirus, as proven by the high viral titre and brain lesions detected, but further studies are necessary to understand if Betanodavirus can cause clinical disease in this species.


Assuntos
Doenças dos Peixes/patologia , Nodaviridae/genética , Perciformes/virologia , Infecções por Vírus de RNA/veterinária , Animais , Suscetibilidade a Doenças , Doenças dos Peixes/virologia , Genótipo , Noruega , Infecções por Vírus de RNA/patologia
9.
Ann Parasitol ; 65(3): 287-291, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31599557

RESUMO

The nematode parasites can cause mechanical injuries such as irritation or atrophy of tissue and occlusions of the digestive tract, blood vessels or other ducts in their hosts. Some species belonging to the family Camallanidae have been the focus of histopathological studies because they lacerate the host´s intestinal wall with their buccal capsule. In this study, we describe the histopathological effects produced by Procamallanus (Spirocamallanus) pintoi in the small fish Corydoras micracanthus from Lesser River, in the northwest of Argentina, South America. The samples were processed with routine histology techniques. At light microscopy, specimens of P. (S.) pintoi were observed occupying the lumen of the gut. The histopathological sections revealed damage to the host intestine with the total destruction of the mucosa and submucosa, observed mainly in the region which is surrounding the buccal capsule of parasites. Moreover, an inflammatory reaction was observed with lymphatic accumulations in the affected tissue. This kind of degenerative and necrotic changes in the mucosa could affect negatively the absorption function of the host intestine, which may have adverse effects of the health status of fish. The present study provides the firs data about the histopathological effects of P. (S.) pintoi in C. micracanthus.


Assuntos
Peixes-Gato , Doenças dos Peixes , Nematoides , Infecções por Nematoides , Animais , Argentina , Peixes-Gato/parasitologia , Doenças dos Peixes/patologia , Infecções por Nematoides/patologia , Infecções por Nematoides/veterinária , Rios
10.
J Fish Dis ; 42(11): 1629-1636, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31578751

RESUMO

Detection of tilapia lake virus (TiLV) in tilapines is mainly from visceral organs of killed fish. However, lethal sampling might not be viable to broodstock and economically important ornamental cichlids. To contribute towards screening of the virus in asymptomatic infected fish, a subclinically infected population of Nile tilapia adults obtained from a local farm was preliminarily tested to compare different non-lethal sampling methods, for example liver biopsy, gill biopsy, fin clip, mucus, faeces and blood for detection of TiLV. Only liver and blood samples gave positive results by PCR. Since blood sampling is relatively simpler, it was further used for five naturally co-cultured juvenile fish species from above-mentioned farm including 40 red tilapia broodstock and 20 Nile tilapia adults from two other different farms. The results showed that from the tested fish, 4 of 5 Nile tilapia, 2 of 5 hybrid red tilapia and 3 of 5 giant gourami blood samples tested positive, while 38 of 40 blood samples of red tilapia tested positive for TiLV in second-step PCR. Sequencing representative PCR amplicons of positive samples confirmed sequence identity to TiLV. In conclusion, both blood and liver biopsy are practical non-destructive sampling platforms for TiLV screening in cichlids with blood being more convenient, especially for tilapia broodstock.


Assuntos
Biópsia/veterinária , Ciclídeos , Doenças dos Peixes/diagnóstico , Infecções por Vírus de RNA/veterinária , Vírus de RNA/isolamento & purificação , Animais , Infecções Assintomáticas , Biópsia/métodos , Sangue/virologia , Doenças dos Peixes/patologia , Fígado/patologia , Fígado/virologia , Infecções por Vírus de RNA/diagnóstico , Infecções por Vírus de RNA/patologia
12.
J Fish Dis ; 42(10): 1401-1408, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31393016

RESUMO

Lumpfish use their vision to hunt prey or, in the case of aquaculture, to see and eat pelleted diets. A common anterior ocular opacity abnormality designated in the literature as "cataract" described in both farmed and wild lumpfish has not yet been characterized in detail at the pathobiological level. We describe here lens tissue changes associated with cataract in cultured and domesticated lumpfish. Methodology included gross observations, ophthalmoscopy and histology. Young adult cultured animals approaching 400 days post-hatch presented a range of anterior segment opacities associated with lenticular abnormalities observable at a histological level. Wild aged domesticated animals also displayed cataracts characterized mainly by abnormalities of the lens observed by both ophthalmoscopy and histology. Dysplastic lesions of the lens in one aged domesticated lumpfish were accompanied with both retinal and optic nerve degeneration. These novel naturally occurring anatomical changes in lumpfish present both commonalities and unique features associated with cataract in young adult cultured animals versus aged domesticated broodstock animals.


Assuntos
Catarata/veterinária , Doenças dos Peixes/patologia , Peixes , Cristalino/patologia , Animais , Catarata/patologia , Perciformes
13.
J Comp Pathol ; 170: 86-90, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31375164

RESUMO

Granulomatous lesions were observed in the swim bladder, kidney, spleen and gills of two farmed Japanese pufferfish (Takifugu rubripes) infected with Mycobacterium chelonae. Three types of lesions were noted: unencapsulated clusters of epithelioid cells without central necrosis (type 1), encapsulated granulomas without central necrosis (type 2) and encapsulated granulomas with central necrosis (type 3). Type 3 lesions occurred most frequently in the swim bladder, while type 1 and type 2 lesions occurred frequently in the kidney and spleen, and the gills exhibited mostly type 1 lesions. This suggests that the lesions in the swim bladder were more fully developed than those occurring elsewhere and that the swim bladder may be more susceptible to infection with M. chelonae. This is the first report describing the histopathological features of M. chelonae infection in Tetraodontidae.


Assuntos
Doenças dos Peixes/microbiologia , Doenças dos Peixes/patologia , Infecções por Mycobacterium não Tuberculosas/veterinária , Tetraodontiformes/microbiologia , Animais , Aquicultura , Japão , Mycobacterium chelonae
14.
Microb Pathog ; 135: 103649, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31374321

RESUMO

Extracellular adenosine triphosphate (ATP) is as key mediator of immune and inflammatory responses. ATP is normally sequestered in the intracellular milieu and released by apoptotic and necrotic cells, where it acts as a pro-inflammatory mediator in the extracellular milieu. A limited number of studies have explored the involvement of purinergic signaling in oomycete infections, including Saprolegnia parasitica; this is a most destructive oomycete pathogen, associated with high mortality and severe economic losses for fish producers. The aim of this study was to determine whether purinergic signaling exerts anti- or pro-inflammatory effects in spleens of grass carp (Ctenopharyngodon idella) naturally infected by S. parasitica. Animals naturally infected with S. parasitica showed typical gross lesions characterized by cotton-wool tufts on the tail and fins, as well as severe histopathological lesions such as necrosis. Spleen ATP and metabolites of nitric oxide (NOx) levels were higher in fish naturally infected by S. parasitica compared to control on day 7 post-infection (PI). Spleen nucleoside triphosphate diphosphohydrolase (NTPDase) activity (ATP as substrate) was greater in fish naturally infected by S. parasitica than in uninfected on day 7 PI, while no significant differences were observed between groups with respect to NTPDase (adenosine diphosphate as substrate) and 5'-nucleotidase activities. Finally, adenosine deaminase (ADA) activity was lower in fish naturally infected by S. parasitica than in uninfected fish on day 7 PI. In summary, spleen tissue necrosis in the context of saprolegniosis provokes an intense release of ATP into the extracellular milieu, where it interacts with the P2X7 purine receptor and leads to a self-sustained pro-inflammatory deleterious cycle, contributing to an intense inflammatory process. In response to excessive ATP levels in the extracellular milieu, ATP and adenosine hydrolysis were modulated in an attempt to restrict the inflammatory process via upregulation of NTPDase and downregulation of ADA activities. We conclude that the purinergic signaling pathway modulates immune and inflammatory responses during natural infection with S. parasitica.


Assuntos
Trifosfato de Adenosina/metabolismo , Anti-Inflamatórios/metabolismo , Carpas/imunologia , Doenças dos Peixes/imunologia , Doenças dos Peixes/parasitologia , Purinérgicos/metabolismo , Transdução de Sinais , Baço/metabolismo , Adenosina Desaminase/metabolismo , Animais , Carpas/metabolismo , Modelos Animais de Doenças , Doenças dos Peixes/patologia , Proteínas de Peixes/imunologia , Micoses , Necrose , Óxido Nítrico/metabolismo , Saprolegnia/patogenicidade , Baço/patologia
15.
J Fish Dis ; 42(10): 1433-1446, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31429104

RESUMO

Amoebic gill disease (AGD) is emerging as one of the most significant health challenges affecting farmed Atlantic salmon in the marine environment. It is caused by the amphizoic amoeba Neoparamoeba perurans, with infestation of gills causing severe hyperplastic lesions, compromising overall gill integrity and function. This study used histology, transmission electron microscopy (TEM), immunohistochemistry and transcript expression to relate AGD-associated pathological changes to changes in the morphology and distribution of chloride cells (CCs) in the gills of Atlantic salmon (Salmo salar L.) showing the progression of an AGD infection. A marked reduction in numbers of immunolabelled CCs was detected, and a changing pattern in distribution and morphology was closely linked with the level of basal epithelial hyperplasia in the gill. In addition, acute degenerative ultrastructural changes to CCs at the lesion site were observed with TEM. These findings were supported by the early-onset downregulation of Na+ /K+ -ATPase transcript expression. This study provides supportive evidence that histological AGD lesion assessment was a good qualitative tool for AGD scoring and corresponded well with qPCR genomic Paramoeba perurans quantification. Ultrastructural changes induced in salmon CCs as a result of AGD are reported here for the first time.


Assuntos
Amebíase/veterinária , Doenças dos Peixes/patologia , Brânquias/patologia , Salmo salar , Amebíase/patologia , Animais , Epitélio/microbiologia , Epitélio/patologia , Epitélio/ultraestrutura , Expressão Gênica/fisiologia , Brânquias/citologia , Brânquias/microbiologia , Brânquias/ultraestrutura , Imuno-Histoquímica/veterinária , Microscopia Eletrônica de Transmissão/veterinária
16.
J Fish Dis ; 42(10): 1419-1424, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31411735

RESUMO

A retrospective study was conducted using 250 clinical records of brown trout (Salmo trutta L.) with saprolegniosis by Saprolegnia parasitica, which had been collected from 8 rivers and 1 fish farm in the province of León (Spain). A geographic information system (GIS) was used to obtain skin lesion distribution patterns in males and females. Lesions in wild brown trout affected 15.31 ± 13.33% of the body surface, with a mean of 12.76 ± 6.56 lesions per fish. In addition, 51.23% of wild trout presented lesions with necrosis of the skin or fins. The pattern obtained when not distinguishing between sexes indicated that saprolegniosis lesions are mainly located above the lateral line and most frequently affect the dorsal cephalic region, the adipose fin, the peduncle and the caudal fin. However, differences were observed between males and females. Farmed trout presented a lower percentage of affected body surface (2.06 ± 4.36) and a lower number of lesions with and without necrosis because they received preventive treatment for saprolegniosis.


Assuntos
Doenças dos Peixes/patologia , Sistemas de Informação Geográfica , Saprolegnia/fisiologia , Dermatopatias/veterinária , Animais , Feminino , Sistemas de Informação Geográfica/estatística & dados numéricos , Masculino , Estudos Retrospectivos , Dermatopatias/patologia , Espanha
17.
Vet Immunol Immunopathol ; 214: 109891, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31378219

RESUMO

Animal models are invaluable tools in cancer research. In this context, salmon is a promising candidate. Intestinal adenocarcinoma with metastases may be induced as a consequence of a plant-based diet triggering the inflammation - dysplasia- carcinogenesis pathway. Here, we investigate the stroma and the presence and nature of immune cells in such tumors by staining for mast cells, immunohistochemistry for T cells and antigen-presenting cells and in situ hybridization for B cells. In intestinal tumors, substantial amounts of T cells were detected in the stroma, whilst MHC class II+ cells were mainly among the cancerous cells. Ig+ cells were observed primarily in the tumor periphery. Mast cells showed a strong association with stroma. In metastases, scarce amounts of T cells were detected, whilst MHC I and II-reactivity varied, some tumors being completely negative. Ig+ cells were scattered around the metastatic tissue in no particular pattern, but were occasionally observed within clusters of tumor cells. Small numbers of mast cells were detected in the stroma. To the best of our knowledge, this is the first report addressing immune cells in fish tumors. The teleost tumor microenvironment seems comparable to that of mammals, making fish interesting model animals in oncoimmunology research.


Assuntos
Adenocarcinoma/veterinária , Doenças dos Peixes/patologia , Neoplasias Intestinais/veterinária , Metástase Neoplásica , Salmo salar/imunologia , Microambiente Tumoral/imunologia , Adenocarcinoma/imunologia , Animais , Células Apresentadoras de Antígenos/imunologia , Modelos Animais de Doenças , Doenças dos Peixes/imunologia , Inflamação , Neoplasias Intestinais/imunologia , Mastócitos/imunologia , Linfócitos T/imunologia
18.
Microb Pathog ; 135: 103617, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31283962

RESUMO

The bluegill sunfish, Lepomis macrochirus, is an important aquacultural and recreational species in southern China because of its excellent taste, rapid growth rate, and good looks. At present, few pathogens are known to affect the bluegill sunfish. However, an iridovirus-like disease recently caused heavy losses to the bluegill sunfish aquaculture industry in Guangdong, China. We report that a virus, designated BSMIV-SD-20171020, was isolated from diseased bluegill sunfish in China. The isolate was efficiently propagated in a Chinese perch brain (CPB) cell line. The cytopathic effect was observed, the MCP gene PCR amplified, and the virus observed with electron microscopy. Its viral titer in CPB cells reached 104.13 TCID50 mL-1. The mortality rate was 100% when bluegill sunfish were challenged with BSMIV-SD-20171020 at a dose of 103.13 TCID50/fish. A histopathological examination revealed basophilic hypertrophied cells in the intestine, liver, and spleen. A nucleotide sequence alignment and phylogenetic analysis of the major capsid protein revealed that isolate BSMIV-SD-20171020 is the species Infectious spleen and kidney necrosis virus (ISKNV), in the genus Megalocytivirus.


Assuntos
Infecções por Vírus de DNA/veterinária , Infecções por Vírus de DNA/virologia , Doenças dos Peixes/virologia , Iridoviridae/classificação , Iridoviridae/isolamento & purificação , Perciformes/virologia , Animais , Aquicultura , Encéfalo , Proteínas do Capsídeo/classificação , Proteínas do Capsídeo/genética , Linhagem Celular , China , Infecções por Vírus de DNA/patologia , Doenças dos Peixes/patologia , Peixes , Iridoviridae/genética , Iridoviridae/patogenicidade , Rim/patologia , Rim/virologia , Fígado/patologia , Fígado/virologia , Percas , Filogenia , Análise de Sequência de DNA/veterinária , Baço/patologia , Baço/virologia
19.
J Vet Diagn Invest ; 31(5): 788-791, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31328699

RESUMO

Pigment cell tumors, also known as chromatophoromas, are cutaneous spindle cell neoplasms originating from pigment cells (chromatophores) in the dermis of teleosts, amphibians, and reptiles. Chromatophoromas share similar histologic morphology to other spindle cell tumors and are not always pigmented. Therefore, immunohistochemical analysis may be useful in distinguishing these neoplasms from tumors of other cellular origin when poorly pigmented. We performed 3 immunohistochemistry assays (PNL-2, melan A, and SOX10) on 8 cutaneous neoplasms from 8 teleosts diagnosed as chromatophoromas based on histologic morphology. Semiquantitative analysis of immunoreactivity was evaluated on each immunohistochemical assay using a 0-3 scale. PNL-2 exhibited mild-to-moderate (1 or 2) immunoreactivity in 7 of the cases, and resident chromatophores (internal control) were also immunoreactive in these cases. Melan A exhibited mild-to-moderate (1 or 2) immunoreactivity in 4 cases (and with resident chromatophores in these cases); SOX10 was not immunoreactive in any cases. Our results indicate that PNL-2 may be a useful marker in teleosts to distinguish tumors of chromatophore origin. Melan A could also be useful, but appears to be less sensitive, and SOX10 is likely not a useful marker for these neoplasms in teleosts.


Assuntos
Carpas , Cromatóforos/patologia , Doenças dos Peixes/patologia , Carpa Dourada , Neoplasias/veterinária , Animais , Imuno-Histoquímica/veterinária , Neoplasias/patologia
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