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2.
J Drugs Dermatol ; 23(7): 551-556, 2024 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-38954627

RESUMO

BACKGROUND: Calcium hydroxylapatite (CaHA) dermal filler is used for a variety of aesthetic treatments; however, the safety and effectiveness of diluted CaHA for the treatment of décolleté wrinkles have not been established. OBJECTIVE: To demonstrate the effectiveness and safety of diluted CaHA (Radiesse; 1:2 CaHA:saline) injection for the improvement of décolleté wrinkles in females. METHODS: Eligible females with moderate or severe ratings on the Merz Aesthetic Scale (MAS) Decollete Wrinkles - At Rest received up to 3 injection cycles of diluted CaHA either 8 weeks apart (3 injection cycles) or 16 weeks apart (2 injection cycles). Effectiveness was evaluated by improvement on the MAS. Adverse events were recorded over a 52 week period. RESULTS: Sixteen weeks after the last treatment, the response rate (1-point improvement or greater) on the MAS Decollete Wrinkles - At Rest was 73.5% (P<0.0001; pooled sample) for all patients. The use of diluted CaHA in the decollete also demonstrated a favorable safety profile. CONCLUSIONS: Diluted CaHA is a safe and effective treatment for the improvement of decollete wrinkles in females.J Drugs Dermatol. 2024;23(7):551-556.  doi:10.36849/JDD.8261.


Assuntos
Preenchedores Dérmicos , Durapatita , Envelhecimento da Pele , Humanos , Feminino , Envelhecimento da Pele/efeitos dos fármacos , Durapatita/administração & dosagem , Durapatita/efeitos adversos , Estudos Prospectivos , Pessoa de Meia-Idade , Preenchedores Dérmicos/administração & dosagem , Preenchedores Dérmicos/efeitos adversos , Resultado do Tratamento , Técnicas Cosméticas , Adulto , Método Simples-Cego , Idoso
3.
Biotechnol J ; 19(7): e2300751, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38987220

RESUMO

The compatibility of bone graft substitutes (BGS) with mesenchymal stem cells (MSCs) is an important parameter to consider for their use in repairing bone defects as it eventually affects the clinical outcome. In the present study, a few commercially available BGS - ß-tricalcium phosphate (ß-TCP), calcium sulfate, gelatin sponge, and different forms of hydroxyapatite (HAP) were screened for their interactions with MSCs from adipose tissue (ADSCs). It was demonstrated that HAP block favorably supported ADSC viability, morphology, migration, and differentiation compared to other scaffolds. The results strongly suggest the importance of preclinical evaluation of bone scaffolds for their cellular compatibility. Furthermore, the bone regenerative potential of HAP block with ADSCs was evaluated in an ex vivo bone defect model developed using patient derived trabecular bone explants. The explants were cultured for 45 days in vitro and bone formation was assessed by expression of osteogenic genes, ALP secretion, and high resolution computed tomography. Our findings confirmed active bone repair process in ex vivo settings. Addition of ADSCs significantly accelerated the repair process and improved bone microarchitecture. This ex vivo bone defect model can emerge as a viable alternative to animal experimentation and also as a potent tool to evaluate patient specific bone therapeutics under controlled conditions.


Assuntos
Tecido Adiposo , Regeneração Óssea , Diferenciação Celular , Células-Tronco Mesenquimais , Engenharia Tecidual , Alicerces Teciduais , Humanos , Tecido Adiposo/citologia , Engenharia Tecidual/métodos , Alicerces Teciduais/química , Células-Tronco Mesenquimais/citologia , Cabeça do Fêmur , Osteogênese , Células Cultivadas , Substitutos Ósseos/química , Durapatita/química , Fosfatos de Cálcio/química
4.
Int J Nanomedicine ; 19: 6359-6376, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38946885

RESUMO

Background: Bone tissue engineering (BTE) is a promising alternative to autologous bone grafting for the clinical treatment of bone defects, and inorganic/organic composite hydrogels as BTE scaffolds are a hot spot in current research. The construction of nano-hydroxyapatite/gelatin methacrylate/oxidized sodium alginate (nHAP/GelMA/OSA), abbreviated as HGO, composite hydrogels loaded with bone morphogenetic protein 7 (BMP7) will provide a suitable 3D microenvironment to promote cell aggregation, proliferation, and differentiation, thus facilitating bone repair and regeneration. Methods: Dually-crosslinked hydrogels were fabricated by combining GelMA and OSA, while HGO hydrogels were formulated by incorporating varying amounts of nHAP. The hydrogels were physically and chemically characterized followed by the assessment of their biocompatibility. BMP7-HGO (BHGO) hydrogels were fabricated by incorporating suitable concentrations of BMP7 into HGO hydrogels. The osteogenic potential of BHGO hydrogels was then validated through in vitro experiments and using rat femoral defect models. Results: The addition of nHAP significantly improved the physical properties of the hydrogel, and the composite hydrogel with 10% nHAP demonstrated the best overall performance among all groups. The selected concentration of HGO hydrogel served as a carrier for BMP7 loading and was evaluated for its osteogenic potential both in vivo and in vitro. The BHGO hydrogel demonstrated superior in vitro osteogenic induction and in vivo potential for repairing bone tissue compared to the outcomes observed in the blank control, BMP7, and HGO groups. Conclusion: Using hydrogel containing 10% HGO appears promising for bone tissue engineering scaffolds, especially when loaded with BMP7 to boost its osteogenic potential. However, further investigation is needed to optimize the GelMA, OSA, and nHAP ratios, along with the BMP7 concentration, to maximize the osteogenic potential.


Assuntos
Alginatos , Proteína Morfogenética Óssea 7 , Regeneração Óssea , Durapatita , Gelatina , Hidrogéis , Osteogênese , Engenharia Tecidual , Alicerces Teciduais , Alginatos/química , Alginatos/farmacologia , Animais , Proteína Morfogenética Óssea 7/química , Proteína Morfogenética Óssea 7/farmacologia , Gelatina/química , Engenharia Tecidual/métodos , Hidrogéis/química , Hidrogéis/farmacologia , Durapatita/química , Durapatita/farmacologia , Osteogênese/efeitos dos fármacos , Ratos , Regeneração Óssea/efeitos dos fármacos , Alicerces Teciduais/química , Ratos Sprague-Dawley , Metacrilatos/química , Masculino , Humanos , Osso e Ossos/efeitos dos fármacos
5.
Int J Mol Sci ; 25(13)2024 Jul 03.
Artigo em Inglês | MEDLINE | ID: mdl-39000425

RESUMO

This study investigated the impact of adding hydroxyapatite nanoparticles to implant surfaces treated with zirconia blasting and acid etching (ZiHa), focusing on structural changes and bone healing parameters in low-density bone sites. The topographical characterization of titanium discs with a ZiHa surface and a commercially modified zirconia-blasted and acid-etched surface (Zi) was performed using scanning electron microscopy, profilometry, and surface-free energy. For the in vivo assessment, 22 female rats were ovariectomized and kept for 90 days, after which one implant from each group was randomly placed in each tibial metaphysis of the animals. Histological and immunohistochemical analyses were performed at 14 and 28 days postoperatively (decalcified lab processing), reverse torque testing was performed at 28 days, and histometry from calcified lab processing was performed at 60 days The group ZiHa promoted changes in surface morphology, forming evenly distributed pores. For bone healing, ZiHa showed a greater reverse torque, newly formed bone area, and bone/implant contact values compared to group Zi (p < 0.05; t-test). Qualitative histological and immunohistochemical analyses showed higher features of bone maturation for ZiHa on days 14 and 28. This preclinical study demonstrated that adding hydroxyapatite to zirconia-blasted and acid-etched surfaces enhanced peri-implant bone healing in ovariectomized rats. These findings support the potential for improving osseointegration of dental implants, especially in patients with compromised bone metabolism.


Assuntos
Durapatita , Nanopartículas , Osseointegração , Propriedades de Superfície , Zircônio , Zircônio/química , Animais , Durapatita/química , Durapatita/farmacologia , Feminino , Ratos , Nanopartículas/química , Osseointegração/efeitos dos fármacos , Implantes Dentários , Titânio/química , Tíbia/efeitos dos fármacos , Tíbia/cirurgia , Condicionamento Ácido do Dente
6.
Langmuir ; 40(28): 14476-14485, 2024 Jul 16.
Artigo em Inglês | MEDLINE | ID: mdl-38967501

RESUMO

Breast cancer is a common malignant tumor arising in normal mammary epithelial tissues. Nearly 75% of the patients with advanced mammary cancer develop bone metastases, resulting in secondary tumor growth, osteolytic bone degradation, and poor prognosis. The bone matrix comprises a highly hierarchical architecture and is composed of a nonmineral organic part, a predominantly type-I collagen, and a mineral inorganic part composed of hydroxyapatite (HA) nanocrystals (Ca10(PO4)6(OH)2). Although there has been extensive research indicating that the material properties of bone minerals affect metastatic breast cancer, it remains unclear how the microenvironment of the bone matrix, such as the roughness, which changes as a result of osteolytic bone remodeling, affects this disease. In this study, we created HA coatings in situ on polyelectrolyte multilayers (PEMs) by incubating PEMs in a mixture of phosphate and calcium ions. The HA films with distinctive roughness were successfully collected by controlling the incubation time, which served as the simulated microenvironment of the bone matrix. MDA-MB231 breast cancer cells were cultured on HA films, and an optimal roughness was observed in the adhesion, proliferation, and expression of two cytokines closely related to bone metastasis. This study contributed to the understanding of the effect of the microenvironment of the bone matrix, such as the roughness, on the metastasis behavior of breast cancer.


Assuntos
Neoplasias Ósseas , Neoplasias da Mama , Durapatita , Durapatita/química , Humanos , Neoplasias da Mama/patologia , Neoplasias Ósseas/secundário , Neoplasias Ósseas/patologia , Linhagem Celular Tumoral , Feminino , Microambiente Tumoral/efeitos dos fármacos , Propriedades de Superfície , Proliferação de Células/efeitos dos fármacos , Adesão Celular/efeitos dos fármacos
7.
ACS Nano ; 18(28): 18425-18443, 2024 Jul 16.
Artigo em Inglês | MEDLINE | ID: mdl-38975713

RESUMO

Tumor in situ vaccination (ISV) strategies have emerged in clinical trials as promising approaches, involving the release of tumor antigens through local radiotherapy and intratumorally adjuvant injections. However, the current fabrication strategy for achieving a sustainable immune response to ISV remains a pressing challenge. In this study, we present an empowered sustainable ISV method for antitumor therapy using 177Lu-labeled manganese-doped mesoporous hydroxyapatite (177Lu/Mn-HAP) microspheres. The ISV enables the sustained utilization of tumor antigens, leading to the activation of dendritic cells and polarization of macrophages toward the M1 subtype. Consequently, it facilitates the generation of potent CD8+ T-cell responses, enhancing the antitumor effects of internal radiation in both primary and distant tumors. Importantly, this approach achieves complete remission in all tumor-bearing mice and stimulates immune memory to prevent tumor recurrence. Our study highlights a universal and safe ISV strategy capable of inducing potent tumor-specific and sustainable immune response.


Assuntos
Vacinas Anticâncer , Durapatita , Microesferas , Durapatita/química , Animais , Camundongos , Vacinas Anticâncer/imunologia , Vacinas Anticâncer/química , Linfócitos T CD8-Positivos/imunologia , Vacinação , Feminino , Camundongos Endogâmicos C57BL , Radioisótopos/química , Linhagem Celular Tumoral
8.
J Gene Med ; 26(7): e3716, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38961849

RESUMO

BACKGROUND: Differentiation of pluripotent stem cells into desired lineages is the key aspect of regenerative medicine and cell-based therapy. Although RNA interference (RNAi) technology is exploited extensively for this, methods for long term silencing of the target genes leading to differentiation remain a challenge. Sustained knockdown of the target gene by RNAi is often inefficient as a result of low delivery efficiencies, protocol induced toxicity and safety concerns related to viral vectors. Earlier, we established octa-arginine functionalized hydroxyapatite nano vehicles (R8HNPs) for delivery of small interfering RNA (siRNA) against a pluripotency marker gene in mouse embryonic stem cells. Although we demonstrated excellent knockdown efficiency of the target gene, sustained gene silencing leading to differentiation was yet to be achieved. METHODS: To establish a sustained non-viral gene silencing protocol using R8HNP, we investigated various methods of siRNA delivery: double delivery of adherent cells (Adh-D), suspension delivery followed by adherent delivery (Susp + Adh), single delivery in suspension (Susp-S) and multiple deliveries in suspension (Susp-R). Sustained knockdown of a pluripotent marker gene followed by differentiation was analysed by reverse transcriptase-PCR, fluoresence-activated cell sorting and immunofluorescence techniques. Impact on cell viability as a result of repeated exposure of the R8HNP was also tested. RESULTS: Amongst the protocols tested, the most efficient knockdown of the target gene for a prolonged period of time was obtained by repeated suspension delivery of the R8HNP-siRNA conjugate. The long-term silencing of a pluripotency marker gene resulted in differentiation of R1 ESCs predominantly towards the extra embryonic and ectodermal lineages. Cells displayed excellent tolerance to repeated exposures of R8HNPs. CONCLUSIONS: The results demonstrate that R8HNPs are promising, biocompatible, non-viral alternatives for prolonged gene silencing and obtaining differentiated cells for therapeutics.


Assuntos
Diferenciação Celular , Durapatita , Células-Tronco Embrionárias Murinas , RNA Interferente Pequeno , Animais , Camundongos , Durapatita/química , Células-Tronco Embrionárias Murinas/metabolismo , Células-Tronco Embrionárias Murinas/efeitos dos fármacos , RNA Interferente Pequeno/genética , Inativação Gênica , Materiais Biocompatíveis/química , Células-Tronco Pluripotentes/citologia , Células-Tronco Pluripotentes/metabolismo , Nanopartículas/química , Transdução Genética , Interferência de RNA , Técnicas de Silenciamento de Genes
9.
J Agric Food Chem ; 72(28): 15523-15529, 2024 Jul 17.
Artigo em Inglês | MEDLINE | ID: mdl-38963614

RESUMO

The eggshell is a composite and highly ordered structure formed by biomineralization. Besides other functions, it has a vital and intricate role in the protection of an embryo from various potentially harsh environmental conditions. Solid-state nuclear magnetic resonance (SSNMR) has been used for detailed structural investigations of the chicken, tinamou, and flamingo eggshell materials. 31P NMR spectra reveal that hydroxyapatite and ß-tricalcium phosphate in the ratio 3:2 represent major constituents of phosphate species in the eggshells. All three eggshells exhibit similar spectra, except for the line widths, which implies different structural order of phosphate species in the chicken, tinamou, and flamingo eggshells. 1H NMR spectra for these materials are comparable, differentiating overlapped peaks in three spectral regions at around 7, 4-5, and 1-2 ppm. These spectral regions have been attributed to protons from NH or CaHCO3, water, and possibly isolated monomeric water molecules or hydroxyl groups in calcium-deficient hydroxyapatite. 1H-13C CP MAS NMR revealed the presence of organic matter in the form of lipids and proteins. Two overlapped resonances in the carbonyl region at around 173 and 169 ppm are assigned to the carbonyls of the peptide bonds and the bicarbonate unit in calcite, respectively. Fourier-transform infrared spectroscopy (FTIR) spectra confirmed the presence of structural units detected in the NMR spectra.


Assuntos
Galinhas , Casca de Ovo , Espectroscopia de Ressonância Magnética , Animais , Casca de Ovo/química , Espectroscopia de Ressonância Magnética/métodos , Durapatita/química , Aves , Fosfatos de Cálcio/química
10.
Sci Rep ; 14(1): 12721, 2024 06 03.
Artigo em Inglês | MEDLINE | ID: mdl-38830871

RESUMO

Surface structure plays a crucial role in determining cell behavior on biomaterials, influencing cell adhesion, proliferation, differentiation, as well as immune cells and macrophage polarization. While grooves and ridges stimulate M2 polarization and pits and bumps promote M1 polarization, these structures do not accurately mimic the real bone surface. Consequently, the impact of mimicking bone surface topography on macrophage polarization remains unknown. Understanding the synergistic sequential roles of M1 and M2 macrophages in osteoimmunomodulation is crucial for effective bone tissue engineering. Thus, exploring the impact of bone surface microstructure mimicking biomaterials on macrophage polarization is critical. In this study, we aimed to sequentially activate M1 and M2 macrophages using Poly-L-Lactic acid (PLA) membranes with bone surface topographical features mimicked through the soft lithography technique. To mimic the bone surface topography, a bovine femur was used as a model surface, and the membranes were further modified with collagen type-I and hydroxyapatite to mimic the bone surface microenvironment. To determine the effect of these biomaterials on macrophage polarization, we conducted experimental analysis that contained estimating cytokine release profiles and characterizing cell morphology. Our results demonstrated the potential of the hydroxyapatite-deposited bone surface-mimicked PLA membranes to trigger sequential and synergistic M1 and M2 macrophage polarizations, suggesting their ability to achieve osteoimmunomodulatory macrophage polarization for bone tissue engineering applications. Although further experimental studies are required to completely investigate the osteoimmunomodulatory effects of these biomaterials, our results provide valuable insights into the potential advantages of biomaterials that mimic the complex microenvironment of bone surfaces.


Assuntos
Macrófagos , Poliésteres , Propriedades de Superfície , Animais , Macrófagos/metabolismo , Macrófagos/efeitos dos fármacos , Macrófagos/imunologia , Bovinos , Poliésteres/química , Camundongos , Materiais Biocompatíveis/química , Materiais Biocompatíveis/farmacologia , Engenharia Tecidual/métodos , Durapatita/química , Citocinas/metabolismo , Osso e Ossos/citologia , Diferenciação Celular/efeitos dos fármacos , Ativação de Macrófagos/efeitos dos fármacos , Adesão Celular/efeitos dos fármacos , Células RAW 264.7 , Polaridade Celular/efeitos dos fármacos , Fêmur , Colágeno Tipo I/metabolismo
11.
J Appl Biomater Funct Mater ; 22: 22808000241251564, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38912599

RESUMO

OBJECTIVES: This study aims to investigate the effect of coating time on the formation of hydroxyapatite (HA) coating layer on ZK60 substrate and understand the biodegradation behavior of the coated alloy for biodegradable implant applications. METHODS: Biodegradable ZK60 alloy was coated by HA layer for different times of 0.5, 1, 2, and 4 h by chemical conversion method. After coating, all the coated specimens were used for immersion test in Hanks' solution to understand the effect of coating time on the degradation behavior of the alloy. The degradation rate of the coated alloy was evaluated by Mg2+ ion quantification and pH change during immersion test. The microstructure of the coating layer was examined by scanning electron microscope (SEM) equipped with an energy-dispersive X-ray spectroscopy (EDS) before and after immersion to understand the degradation behavior of the coated alloy. RESULTS: HA coating layers were formed successfully on surface of ZK60 specimens after 0.5, 1, 2, and 4 h with different microstructure. Optimal coating quality was observed at 1 or 2 h, characterized by well-formed and uniform HA layers. However, extending the coating duration to 4 h led to the formation of cracks within the HA layer, accompanied by Mg(OH)2. Specimens coated for 1 and 2 h exhibited the lowest degradation rates, while specimens coated for 0.5 and 4 h showed the highest degradation rates. Furthermore, analysis of degradation products revealed the predominance of calcium phosphates formed on the surface of specimens coated for 1 and 2 h. Conversely, specimens coated for 0.5 and 4 h exhibited Mg(OH)2 as the primary degradation product, suggesting a less effective corrosion barrier under these conditions. CONCLUSION: The HA layer formed after 2 h demonstrated as the most effective coating layer for enhancing the corrosion resistance of the ZK60 alloy for biomedical applications.


Assuntos
Ligas , Materiais Revestidos Biocompatíveis , Durapatita , Durapatita/química , Ligas/química , Materiais Revestidos Biocompatíveis/química , Teste de Materiais , Corrosão , Magnésio/química
12.
Sci Rep ; 14(1): 14702, 2024 06 26.
Artigo em Inglês | MEDLINE | ID: mdl-38926433

RESUMO

The aim of this study is to introduce a dental capping agent for the treatment of pulp inflammation (pulpitis). Nanohydroxyapatite with Elaeagnus angustifolia L. extract (nHAEA) loaded with metronidazole (nHAEA@MTZ) was synthesized and evaluated using a lipopolysaccharide (LPS) in vitro model of pulpitis. nHAEA was synthesized through sol-gel method and analyzed using Scanning Electron Microscopy, Transmission Electron Microscopy, and Brunauer Emmett Teller. Inflammation in human dental pulp stem cells (HDPSCs) induced by LPS. A scratch test assessed cell migration, RT PCR measured cytokines levels, and Alizarin red staining quantified odontogenesis. The nHAEA nanorods were 17-23 nm wide and 93-146 nm length, with an average pore diameter of 27/312 nm, and a surface area of 210.89 m2/g. MTZ loading content with controlled release, suggesting suitability for therapeutic applications. nHAEA@MTZ did not affect the odontogenic abilities of HDPSCs more than nHAEA. However, it was observed that nHAEA@MTZ demonstrated a more pronounced anti-inflammatory effect. HDPSCs treated with nanoparticles exhibited improved migration compared to other groups. These findings demonstrated that nHAEA@MTZ could be an effective material for pulp capping and may be more effective than nHAEA in reducing inflammation and activating HDPSCs to enhance pulp repair after pulp damage.


Assuntos
Polpa Dentária , Durapatita , Metronidazol , Extratos Vegetais , Pulpite , Extratos Vegetais/farmacologia , Extratos Vegetais/química , Humanos , Pulpite/tratamento farmacológico , Pulpite/metabolismo , Pulpite/patologia , Metronidazol/farmacologia , Polpa Dentária/efeitos dos fármacos , Polpa Dentária/metabolismo , Polpa Dentária/citologia , Durapatita/química , Nanopartículas/química , Química Verde , Portadores de Fármacos/química , Células-Tronco/efeitos dos fármacos , Células-Tronco/metabolismo , Movimento Celular/efeitos dos fármacos , Células Cultivadas
13.
J Mater Sci Mater Med ; 35(1): 37, 2024 Jun 25.
Artigo em Inglês | MEDLINE | ID: mdl-38916635

RESUMO

The current clinical application of glaucoma drainage devices is made of non-degradable materials. These non-degradable drainage devices often trigger inflammatory responses and scar proliferation, possibly leading to surgical failure. We developed a biodegradable material hydroxyapatite-coated magnesium (HA-Mg) as a glaucoma drainage device. Twelve New Zealand white rabbits were randomly assigned to three groups: HA-Mg drainage plate group (6 right eyes), trabeculectomy group (6 right eyes), and control group (12 left eyes). Results showed that all HA-Mg drainage plates were completely degraded ~4 months postoperatively. At the 5th month postoperatively, there was no statistical difference in the corneal endothelium density between the HA-Mg drainage plate group and the control group (p = 0.857). The intraocular pressure (IOP) level in the HA-Mg drainage plate implantation group was lower than in the other two groups. The trypan blue dye still drained from the anterior chamber to the subconjunctiva 5 months after HA-Mg drainage plate implantation. HE staining revealed the scleral linear aqueous humor drainage channel and anterior synechia were observed after drainage plate completely degraded, with no obvious infiltration with the inflammatory cells. This study showed the safety and efficacy of HA-Mg glaucoma drainage plate in controlling IOP after implantation into the anterior chamber of rabbit eyes.


Assuntos
Câmara Anterior , Implantes para Drenagem de Glaucoma , Glaucoma , Pressão Intraocular , Magnésio , Animais , Coelhos , Câmara Anterior/cirurgia , Glaucoma/cirurgia , Magnésio/química , Durapatita/química , Trabeculectomia/métodos
14.
Int J Mol Sci ; 25(12)2024 Jun 11.
Artigo em Inglês | MEDLINE | ID: mdl-38928145

RESUMO

Polyurethane (PU) is a promising material for addressing challenges in bone grafting. This study was designed to enhance the bone grafting capabilities of PU by integrating hydroxyapatite (HAp), which is known for its osteoconductive and osteoinductive potential. Moreover, a uniform distribution of HAp in the porous structure of PU increased the effectiveness of bone grafts. PEG/APTES-modified scaffolds were prepared through self-foaming reactions. A uniform pore structure was generated during the spontaneous foaming reaction, and HAp was uniformly distributed in the PU structure (PU15HAp and PU30HAp) during foaming. Compared with the PU scaffolds, the HAp-modified PU scaffolds exhibited significantly greater protein absorption. Importantly, the effect of the HAp-modified PU scaffold on bone repair was tested in a rat calvarial defect model. The microstructure of the newly formed bone was analyzed with microcomputed tomography (µ-CT). Bone regeneration at the defect site was significantly greater in the HAp-modified PU scaffold group than in the PU group. This innovative HAp-modified PU scaffold improves current bone graft materials, providing a promising avenue for improved bone regeneration.


Assuntos
Regeneração Óssea , Durapatita , Poliuretanos , Crânio , Alicerces Teciduais , Poliuretanos/química , Animais , Durapatita/química , Alicerces Teciduais/química , Ratos , Regeneração Óssea/efeitos dos fármacos , Crânio/efeitos dos fármacos , Crânio/lesões , Crânio/patologia , Crânio/metabolismo , Ratos Sprague-Dawley , Microtomografia por Raio-X , Masculino , Porosidade , Transplante Ósseo/métodos
15.
Int J Mol Sci ; 25(12)2024 Jun 14.
Artigo em Inglês | MEDLINE | ID: mdl-38928293

RESUMO

Zr-50Ti alloys are promising biomaterials due to their excellent mechanical properties and low magnetic susceptibility. However, Zr-50Ti alloys do not inherently bond well with bone. This study aims to enhance the bioactivity and bonding strength of Zr-50Ti alloys for orthopedic implant materials. Initially, the surface of Zr-50Ti alloys was treated with a sulfuric acid solution to create a microporous structure, increasing surface roughness and area. Subsequently, low crystalline calcium phosphate (L-CaP) precipitation was controlled by adding Mg2+ and/or CO32- ions in modified simulated body fluid (m-SBF). The treated Zr-50Ti alloys were then subjected to cold isostatic pressing to force m-SBF into the micropores, followed by incubation to allow L-CaP formation. The apatite-forming process was tested in simulated body fluid (SBF). The results demonstrated that the incorporation of Mg2+ and/or CO32- ions enabled the L-CaP to cover the entire surface of Zr-50Ti alloys within only one day. After short-term soaking in SBF, the L-CaP layer, modulated by Mg2+ and/or CO32- ions, formed a uniform hydroxyapatite (HA) coating on the surface of the Zr-50Ti alloys, showing potential for optimized bone integration. After soaking in SBF for 14 days, the bonding strength between the apatite layer and alloy has the potential to meet the orthopedic application requirement of 22 MPa. This study demonstrates an effective method to enhance the bioactivity and bonding strength of Zr-50Ti alloys for orthopedic applications.


Assuntos
Ligas , Líquidos Corporais , Fosfatos de Cálcio , Propriedades de Superfície , Zircônio , Ligas/química , Zircônio/química , Líquidos Corporais/química , Fosfatos de Cálcio/química , Titânio/química , Materiais Biocompatíveis/química , Teste de Materiais , Magnésio/química , Durapatita/química
16.
Cell Mol Biol (Noisy-le-grand) ; 70(6): 135-141, 2024 Jun 05.
Artigo em Inglês | MEDLINE | ID: mdl-38836669

RESUMO

Epigenetic change has been found to play an important role in cell differentiation and regulation and the dental pulp stem cell in tissue engineering is gaining attention due to the ability of cells to differentiate into odontoblast and other cells. This study evaluated the influence of poly L- lactic acid with hydroxyapatite-coated with polyaniline scaffold (PLLA/HA/PANI) on dental pulp stem cell (DPSC) proliferation and differentiation. After scaffold preparation and DPSCs seeding, the cells proliferation and differentiation were evaluated by immunocytochemistry assay and cell viability was measured by cytotoxicity / MTT assay. The results showed (PLLA/HA/PANI) scaffold facilitates DPSC proliferation and differentiation with gene expression. This finding underscores the promise of this biomaterial combination as a scaffold for dental tissue regeneration and application.


Assuntos
Materiais Biocompatíveis , Diferenciação Celular , Proliferação de Células , Polpa Dentária , Durapatita , Odontoblastos , Osteoblastos , Células-Tronco , Alicerces Teciduais , Polpa Dentária/citologia , Humanos , Diferenciação Celular/efeitos dos fármacos , Odontoblastos/citologia , Odontoblastos/efeitos dos fármacos , Odontoblastos/metabolismo , Alicerces Teciduais/química , Células-Tronco/citologia , Células-Tronco/metabolismo , Células-Tronco/efeitos dos fármacos , Osteoblastos/citologia , Osteoblastos/efeitos dos fármacos , Osteoblastos/metabolismo , Proliferação de Células/efeitos dos fármacos , Materiais Biocompatíveis/química , Materiais Biocompatíveis/farmacologia , Durapatita/química , Durapatita/farmacologia , Compostos de Anilina/farmacologia , Compostos de Anilina/química , Poliésteres/química , Poliésteres/farmacologia , Sobrevivência Celular/efeitos dos fármacos , Células Cultivadas , Engenharia Tecidual/métodos
17.
Biointerphases ; 19(3)2024 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-38836787

RESUMO

Titanium (Ti) is widely utilized as an implant material; nonetheless, its integration with bone tissue faces limitations due to a patient's comorbidities. To address this challenge, we employed a strategic approach involving the growth of thin films by spin-coating and surface functionalization with etidronate (ETI), alendronate (ALE), and risedronate (RIS). Our methodology involved coating of Ti cp IV disks with thin films of TiO2, hydroxyapatite (HA), and their combinations (1:1 and 1:2 v/v), followed by surface functionalization with ETI, ALE, and RIS. Bisphosphonate-doped films were evaluated in terms of surface morphology and physical-chemical properties by techniques such as electron microscopy, confocal microscopy, and x-ray photoelectron spectroscopy. The antibacterial potential of bisphosphonates alone or functionalized onto the Ti surface was tested against Staphylococcus aureus biofilms. Primary human bone mesenchymal stem cells were used to determine in vitro cell metabolism and mineralization. Although RIS alone did not demonstrate any antibacterial effect as verified by minimum inhibitory concentration assay, when Ti surfaces were functionalized with RIS, partial inhibition of Staphylococcus aureus growth was noted, probably because of the physical-chemical surface properties. Furthermore, samples comprising TiO2/HA (1:1 and 1:2 v/v) showcased an enhancement in the metabolism of nondifferentiated cells and can potentially enhance the differentiation of osteoblastic precursors. All samples demonstrated cell viability higher than 80%. Addition of hydroxyapatite and presence of bisphosphonates increase the metabolic activity and the mineralization of human bone mesenchymal cells. While these findings hold promise, it is necessary to conduct further studies to evaluate the system's performance in vivo and ensure its long-term safety. This research marks a significant stride toward optimizing the efficacy of titanium implants through tailored surface modifications.


Assuntos
Antibacterianos , Difosfonatos , Células-Tronco Mesenquimais , Testes de Sensibilidade Microbiana , Staphylococcus aureus , Propriedades de Superfície , Titânio , Titânio/química , Titânio/farmacologia , Humanos , Antibacterianos/farmacologia , Antibacterianos/química , Staphylococcus aureus/efeitos dos fármacos , Difosfonatos/química , Difosfonatos/farmacologia , Células-Tronco Mesenquimais/efeitos dos fármacos , Biofilmes/efeitos dos fármacos , Materiais Revestidos Biocompatíveis/química , Materiais Revestidos Biocompatíveis/farmacologia , Células Cultivadas , Durapatita/química , Durapatita/farmacologia
18.
Curr Protoc ; 4(6): e1068, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38837274

RESUMO

Adeno-associated virus (AAV) vectors can efficiently transduce exogenous genes into various tissues in vivo. Owing to their convenience, high efficiency, long-term stable gene expression, and minimal side effects, AAV vectors have become one of the gold standards for investigating gene functions in vivo, especially in non-clinical studies. However, challenges persist in efficiently preparing a substantial quantity of high-quality AAV vectors. Commercial AAV vectors are typically associated with high costs. Further, in-laboratory production is hindered by the lack of specific laboratory equipment, such as ultracentrifuges. Therefore, a simple, quick, and scalable preparation method for AAV vectors is needed for proof-of-concept experiments. Herein, we present an optimized method for producing and purifying high-quality AAV serotype 9 (AAV9) vectors using standard laboratory equipment and chromatography. Using ceramic hydroxyapatite as a mixed-mode chromatography medium can markedly increase the quality of purified AAV vectors. Basic Protocols and optional methods for evaluating purified AAV vectors are also described. © 2024 The Author(s). Current Protocols published by Wiley Periodicals LLC. Basic Protocol 1: Production of AAV9 vectors in 293EB cells Basic Protocol 2: Concentration and buffer exchange of AAV9 vectors from 293EB cell culture supernatants using tangential flow filtration Basic Protocol 3: Purification of AAV9 vectors from TFF samples using ceramic hydroxyapatite chromatography Basic Protocol 4: Analysis of the purified AAV9 vectors.


Assuntos
Cerâmica , Dependovirus , Durapatita , Vetores Genéticos , Sorogrupo , Dependovirus/genética , Dependovirus/isolamento & purificação , Vetores Genéticos/isolamento & purificação , Vetores Genéticos/genética , Humanos , Cerâmica/química , Durapatita/química , Cromatografia/métodos , Células HEK293
19.
BMC Musculoskelet Disord ; 25(1): 455, 2024 Jun 08.
Artigo em Inglês | MEDLINE | ID: mdl-38851675

RESUMO

BACKGROUND: Masquelet membrane induction technology is one of the treatment strategies for large bone defect (LBD). However, the angiogenesis ability of induced membrane decreases with time and autologous bone grafting is associated with donor site morbidity. This study investigates if the PRP-FG-nHA/PA66 scaffold can be used as a spacer instead of PMMA to improve the angiogenesis ability of induced membrane and reduce the amount of autologous bone graft. METHODS: Platelet rich plasma (PRP) was prepared and PRP-FG-nHA/PA66 scaffold was synthesized and observed. The sustained release of VEGFA and porosity of the scaffold were analyzed. We established a femur LBD model in male SD rats. 55 rats were randomly divided into four groups depending on the spacer filled in the defect area. "Defect only" group (n = 10), "PMMA" group (n = 15), "PRP-nHA/PA66" group (n = 15) and "PRP-FG-nHA/PA66" group (n = 15 ). At 6 weeks, the spacers were removed and the defects were grafted. The induced membrane and bone were collected and stained. The bone formation was detected by micro-CT and the callus union was scored on a three point system. RESULTS: The PRP-FG-nHA/PA66 scaffold was porosity and could maintain a high concentration of VEGFA after 30 days of preparation. The induced membrane in PRP-FG-nHA/PA66 group was thinner than PMMA, but the vessel density was higher.The weight of autogenous bone grafted in PRP-FG-nHA/PA66 group was significantly smaller than that of PMMA group. In PRP-FG-nHA/PA66 group, the bone defect was morphologically repaired. CONCLUSION: The study showed that PRP-FG-nHA/PA66 scaffold can significantly reduce the amount of autologous bone graft, and can achieve similar bone defect repair effect as PMMA. Our findings provide some reference and theoretical support for the treatment of large segmental bone defects in humans.


Assuntos
Fêmur , Plasma Rico em Plaquetas , Ratos Sprague-Dawley , Alicerces Teciduais , Animais , Masculino , Ratos , Fêmur/cirurgia , Fêmur/patologia , Fator A de Crescimento do Endotélio Vascular , Regeneração Óssea/fisiologia , Neovascularização Fisiológica , Transplante Ósseo/métodos , Durapatita/química , Modelos Animais de Doenças , Osteogênese/fisiologia
20.
Skin Res Technol ; 30(6): e13764, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38853456

RESUMO

Injectable fillers, pivotal in aesthetic medicine, have evolved significantly with recent trends favoring biostimulators like calcium hydroxylapatite (CaHA-CMC; Radiesse, Merz Aesthetics, Raleigh, NC) and poly-l-lactic acid (PLLA; Sculptra Aesthetics, Galderma, Dallas, TX). This study aims to compare the particle morphology of these two injectables and examine its potential clinical implications. Utilizing advanced light and scanning electron microscopy techniques, the physical characteristics of CaHA-CMC and PLLA particles were analyzed, including shape, size, circularity, roundness, aspect ratio, and quantity of phagocytosable particles. The findings reveal several morphological contrasts: CaHA-CMC particles exhibited a smooth, homogenous, spherical morphology with diameters predominantly ranging between 20 and 45 µm, while PLLA particles varied considerably in shape and size, appearing as micro flakes ranging from 2 to 150 µm in major axis length. The circularity and roundness of CaHA-CMC particles were significantly higher compared to PLLA, indicating a more uniform shape. Aspect ratio analysis further underscored these differences, with CaHA-CMC particles showing a closer resemblance to circles, unlike the more oblong PLLA particles. Quantification of the phagocytosable content of both injectables revealed a higher percentage of phagocytosable particles in PLLA. These morphological distinctions may influence the tissue response to each treatment. CaHA-CMC's uniform, spherical particles may result in reduced inflammatory cell recruitment, whereas PLLA's heterogeneous particle morphology may evoke a more pronounced inflammatory response.


Assuntos
Preenchedores Dérmicos , Durapatita , Poliésteres , Durapatita/química , Poliésteres/química , Preenchedores Dérmicos/química , Preenchedores Dérmicos/administração & dosagem , Humanos , Técnicas Cosméticas , Tamanho da Partícula , Materiais Biocompatíveis/química , Microscopia Eletrônica de Varredura
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