Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 413
Filtrar
1.
Biomed Pharmacother ; 118: 109347, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31545273

RESUMO

λ-Carrageenan (Carr), a seaweed polysaccharide, is used as a proinflammatory agent in research. Betulinic acid (BA), a naturally occurring pentacyclic triterpenoid, exerts immunomodulatory, antioxidant, anti-inflammatory, antitumor, anti-malarial and anti-HIV effects. The aim of this study was to investigate whether BA exerts anti-inflammatory effect against Carr-induced paw edema in mice, and how BA could mediate the expression of inflammation-associated MAPK-COX-2-PGE2 signal pathway. BA pretreatment significantly reduced the inflammatory response to Carr-induced paw edema, especially at 4 h after injection. BA reduced the serum levels of pro-inflammatory cytokines, such as IL-1α, IL-1ß, IL-5, IL-6, GM-CSF, KC, MCP-1 and PGE2 in Carr-treated mice, and increased those of anti-inflammatory cytokines, such as IL-12. It also increased SOD, CAT and GSH-Px activities, and GSH content, and reduced MDA content in the liver of Carr-treated mice. Besides, BA reduced neutrophil infiltration in the basal and subcutaneous layers of the paw of Carr-treated mice, decreased the expression of COX-2 protein, and reduced the phosphorylation of JNK, p38 and ERK1/2. These results indicated that the protective effect of BA on Carr-induced paw edema might be due to its alleviation of inflammatory response and inhibition of oxidative stress, possibly by inhibiting MAPK-COX-2-PGE2 signaling pathway activation.


Assuntos
Anti-Inflamatórios/uso terapêutico , Edema/tratamento farmacológico , Extremidades/patologia , Triterpenos/uso terapêutico , Animais , Anti-Inflamatórios/farmacologia , Antioxidantes/metabolismo , Carragenina , Ciclo-Oxigenase 2/metabolismo , Citocinas/sangue , Dinoprostona/sangue , Edema/sangue , Edema/induzido quimicamente , Fígado/efeitos dos fármacos , Fígado/metabolismo , Masculino , Camundongos , Fosforilação/efeitos dos fármacos , Triterpenos/farmacologia
2.
Biol Pharm Bull ; 42(8): 1419-1422, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31366877

RESUMO

The pathogenic relationship of ulcerative colitis and rheumatoid arthritis is not known. Therefore, we examined dextran sodium sulfate (DSS)-induced colitis separately and in combination with a mouse arthritis model that mimics rheumatoid arthritis and evaluated the deterioration-related factors of each condition. Arthritis was induced in a collagen-induced arthritis mouse model using DBA/1JJmsSlc mice and ulcerative colitis was induced by the administration of drinking water containing 3.0% (w/v) DSS. The arthritis/DSS-treated mice developed worse colitis scores compared to that of the other groups of mice. The arthritis/DSS-treated mice did not demonstrate changes in hind foot volumes or in the concentration of matrix metalloproteinase-3 (MMP-3) in the plasma; however, plasma levels of interleukin-6 (IL-6) and tumor necrosis factor (TNF)-α were increased. Our results showed that IL-6 and TNF-α may influence the deterioration effect of colitis in arthritic mice.


Assuntos
Artrite Experimental/imunologia , Colite Ulcerativa/imunologia , Interleucina-6/sangue , Fator de Necrose Tumoral alfa/sangue , Animais , Artrite Experimental/sangue , Artrite Experimental/patologia , Colite Ulcerativa/sangue , Colite Ulcerativa/induzido quimicamente , Colite Ulcerativa/patologia , Colo/patologia , Sulfato de Dextrana , Edema/sangue , Edema/imunologia , Edema/patologia , Pé/patologia , Metaloproteinase 3 da Matriz/sangue , Camundongos Endogâmicos DBA
3.
Int J Mol Sci ; 20(16)2019 Aug 09.
Artigo em Inglês | MEDLINE | ID: mdl-31404946

RESUMO

Heart failure (HF) patients frequently have elevated plasma renin activity. We examined the significance of elevated plasma renin activity in a translationally-relevant model of dilated cardiomyopathy (DCM), which replicates the progressive stages (A-D) of human HF. Female mice with DCM and elevated plasma renin activity concentrations were treated with a direct renin inhibitor (aliskiren) in a randomized, blinded fashion beginning at Stage B HF. By comparison to controls, aliskiren treatment normalized pathologically elevated plasma renin activity (p < 0.001) and neprilysin levels (p < 0.001), but did not significantly alter pathological changes in plasma aldosterone, angiotensin II, atrial natriuretic peptide, or corin levels. Aliskiren improved cardiac systolic function (ejection fraction, p < 0.05; cardiac output, p < 0.01) and significantly reduced the longitudinal development of edema (extracellular water, p < 0.0001), retarding the transition from Stage B to Stage C HF. The normalization of elevated plasma renin activity reduced the loss of body fat and lean mass (cachexia/sarcopenia), p < 0.001) and prolonged survival (p < 0.05). In summary, the normalization of plasma renin activity retards the progression of experimental HF by improving cardiac systolic function, reducing the development of systemic edema, cachexia/sarcopenia, and mortality. These data suggest that targeting pathologically elevated plasma renin activity may be beneficial in appropriately selected HF patients.


Assuntos
Amidas/uso terapêutico , Cardiomiopatia Dilatada/tratamento farmacológico , Fumaratos/uso terapêutico , Renina/antagonistas & inibidores , Renina/sangue , Animais , Caquexia/sangue , Caquexia/complicações , Caquexia/tratamento farmacológico , Cardiomiopatia Dilatada/sangue , Cardiomiopatia Dilatada/complicações , Modelos Animais de Doenças , Edema/sangue , Edema/complicações , Edema/tratamento farmacológico , Feminino , Insuficiência Cardíaca/sangue , Insuficiência Cardíaca/complicações , Insuficiência Cardíaca/tratamento farmacológico , Humanos , Camundongos , Camundongos Endogâmicos C57BL
4.
Mediators Inflamm ; 2019: 3041438, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31263381

RESUMO

Despite the usefulness of glucocorticoids, they may cause hazardous side effects that limit their use. Searching for compounds that are as equally efficient as glucocorticoids, but with less side effects, the current study compared plant steroids, namely, glycyrrhetinic acid, guggulsterone, boswellic acid, withaferin A, and diosgenin with the classical glucocorticoid, fluticasone. This was approached both in silico using molecular docking against glucocorticoid receptor (GR) and in vivo in two different animal models. All tested compounds interacted with GR, but only boswellic acid and withaferin A showed docking results comparable to fluticasone, as well as similar in vivo anti-inflammatory effects, by significantly decreasing serum levels of interleukin-6 and tumor necrosis factor-α in cotton pellet-induced granuloma in rats. In addition, both compounds significantly decreased the percent of change in ear weight in croton oil-induced ear edema in mice and the granuloma weight in cotton pellet-induced granuloma in rats, to levels comparable to that of fluticasone. Both boswellic acid and withaferin A had no effect on adrenal index, but only withaferin A significantly increased the thymus index. In conclusion, boswellic acid may have comparable anti-inflammatory effects to fluticasone with fewer side effects.


Assuntos
Otopatias/tratamento farmacológico , Otopatias/metabolismo , Inflamação/tratamento farmacológico , Inflamação/metabolismo , Fitosteróis/uso terapêutico , Receptores de Glucocorticoides/metabolismo , Animais , Anti-Inflamatórios/uso terapêutico , Óleo de Cróton/toxicidade , Diosgenina/uso terapêutico , Otopatias/sangue , Otopatias/induzido quimicamente , Edema/sangue , Edema/induzido quimicamente , Edema/tratamento farmacológico , Edema/metabolismo , Ensaio de Imunoadsorção Enzimática , Ácido Glicirretínico/uso terapêutico , Inflamação/induzido quimicamente , Inflamação/imunologia , Interleucina-6/sangue , Camundongos , Simulação de Acoplamento Molecular , Pregnenodionas/uso terapêutico , Ratos , Software , Timo/efeitos dos fármacos , Timo/metabolismo , Triterpenos/uso terapêutico , Fator de Necrose Tumoral alfa/sangue , Vitanolídeos/uso terapêutico
5.
J Med Invest ; 66(1.2): 112-118, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31064921

RESUMO

Polymyalgia rheumatica (PMR) and remitting seronegative symmetrical synovitis with pitting edema (RS3PE) syndrome are common inflammatory rheumatic diseases in the elderly. In this study, we investigate predictive factors which correspond to subsequent disease control of PMR and RS3PE syndrome. Twenty four patients, which contained 18 PMR and 6 RS3PE syndrome, were treated with initial dosages of 10-20 mg per day oral prednisolone, and the dosage of prednisolone was then tapered. Significantly higher initial CRP was observed in patients with poor disease control than in those with good disease control afterwards. The number of patients with negative CRP after 4 weeks was significantly more in patients with good disease control after 1 year than in those with poor disease control. Patients were shown to be in good disease control status after 1 year when CRP after 4 weeks became negative even if they had initial high CRP. Our study clarify that to make CRP negative after 4 weeks is associated with subsequent suppression of the disease activity and with decreased dosages of corticosteroids. J. Med. Invest. 66 : 112-118, February, 2019.


Assuntos
Edema/tratamento farmacológico , Polimialgia Reumática/tratamento farmacológico , Prednisolona/uso terapêutico , Sinovite/tratamento farmacológico , Idoso , Idoso de 80 Anos ou mais , Proteína C-Reativa/análise , Edema/sangue , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Polimialgia Reumática/sangue , Estudos Retrospectivos , Síndrome , Sinovite/sangue
6.
Indian J Pharmacol ; 51(1): 25-30, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31031464

RESUMO

OBJECTIVE: Bauhinia purpurea (BP) Linn. (Caesalpiniaceae) is a plant of great medicinal importance and has been used since ancient times for treating many inflammatory conditions including arthritis. This study investigates the anti-arthritic potential of the hydroalcoholic extract from the stem bark of BP. MATERIALS AND METHODS: The anti-inflammatory and anti-arthritic activity of BP at various doses was used to evaluate its anti-inflammatory activity and anti-arthritic activity. Serum of arthritic rats was collected at day 21 for detecting serum cytokines level and to evaluate the effect of BP on its serum level. Furthermore, the safety of BP was evaluated in acute (5 days) and subacute (28 days) toxicity study in rats. RESULTS: There was a significant inhibition (P < 0.01) in paw edema at a different time scale with different doses of BP (50, 100, and 200 mg/kg). BP also demonstrated dose-dependent anti-arthritic activity on all observation days (3, 7, 14, and 21). In addition, there was also a significant decrease (P < 0.01) in oxidative stress markers, circulating pro-inflammatory cytokine (tumor necrosis factor alpha from 45.91 to 37.44, interleukin-1 (IL-1) ß from 18.24 to 16.06, and IL-6 from 69.77 to 58.44) and an increase in anti-inflammatory cytokine (IL-10 from 8.07 to 12.07) levels. BP was found to be safe with an oral LD50 value of >2 g/kg in acute toxicity study and also no toxicological effect was observed in the oral subacute toxicity study. CONCLUSION: This study demonstrates that BP bark possesses anti-arthritic activity potential and confirm its folklore use in the treatment of inflammatory conditions.


Assuntos
Anti-Inflamatórios/uso terapêutico , Artrite Experimental/tratamento farmacológico , Bauhinia , Extratos Vegetais/uso terapêutico , Animais , Anti-Inflamatórios/farmacologia , Artrite Experimental/sangue , Citocinas/sangue , Edema/sangue , Edema/tratamento farmacológico , Masculino , Extratos Vegetais/farmacologia , Ratos Wistar , Testes de Toxicidade Subaguda
7.
Indian Heart J ; 71(1): 32-38, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31000180

RESUMO

BACKGROUND: Despite several decades of use of calcium channel blockers, the side effect of edema persists as a class effect, and its mechanism is unresolved. Amlodipine has effects on hemorheology (HR), and its hemodilutory property may partly contribute to its antihypertensive action. This aspect is not well studied, and the literature is sparse in this regard. OBJECTIVE: This experiment was planned to determine effect of a single-dose administration of amlodipine on HR parameters in normal human volunteers. METHODS AND RESULTS: Amlodipine (5 mg) or S (-) amlodipine (2.5 mg) was administered to 27 normal human volunteers. Whole-blood viscosity (WBV) at different shear rates, plasma viscosity (PV), red cell rigidity (RCR), red cell aggregation (RCA), hematocrit (Hct), plasma hemoglobin, along with plasma drug concentration were determined at time intervals, t = 0, 4, 8, 12, and 24 h. Statistically significant reductions were observed at tmax = 4 h in WBV at shear rates of 0.512 s-1 (p < 0.005), WBV at shear rates of 5.26 s-1 (p < 0.01), PV (p < 0.05), and Hct (p < 0.01). At t = 8 h, as drug concentration reduced, some of the changes persisted and later slowly decreased with the decreasing drug concentration till t = 24 h. Red blood cell-related parameters such as RCA and RCR remained unaltered. WBV values at all shear rates, when corrected for Hct = 0.45, did not show deviation from their original values at any time. CONCLUSIONS: Amlodipine causes a reduction in Hct and blood viscosity, along with hemodilution. These effects persist as long as the drug remains in plasma. Edema resulting from chronic dosing may be explained by the aforementioned effects. It is possible that antihypertensive action of the drug may be due to a combination of vasodilatation and an improvement in the HR properties.


Assuntos
Anlodipino/administração & dosagem , Viscosidade Sanguínea/efeitos dos fármacos , Edema/sangue , Agregação Eritrocítica/efeitos dos fármacos , Hipertensão/tratamento farmacológico , Bloqueadores dos Canais de Cálcio/administração & dosagem , Relação Dose-Resposta a Droga , Edema/tratamento farmacológico , Edema/etiologia , Voluntários Saudáveis , Hematócrito , Humanos , Hipertensão/sangue , Hipertensão/complicações , Masculino , Método Simples-Cego , Resultado do Tratamento , Adulto Jovem
8.
J Stroke Cerebrovasc Dis ; 28(6): 1718-1725, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30922669

RESUMO

OBJECTIVE: Intracerebral hemorrhage affects approximately 2 million individuals per year. While the incidence is roughly equal in men and women, few studies have examined the influence of sex on secondary injury and associated long-term functional outcomes. Matrix metalloproteinases (MMPs) promote vessel rupture and worsen outcomes by potentiating blood-brain barrier breakdown after injury. We hypothesized that different MMP isoform levels would be predictive of injury severity, secondary injury, and long-term functional outcomes in males and females, respectively. METHODS: We examined the levels of MMP isoforms in serum samples from a prospective patient biobank (n = 55). Baseline clinical, radiographic, and laboratory data were also analyzed. RESULTS: We found that MMP-1 (P = .036), MMP-2 (P = .014), MMP-3 (P < .001), and MMP-9 (P = .02) levels gradually increased over time in male patients until 10 DPI. In female patients, we found a different pattern of activation: MMP-8 (P = .02) was the only isoform that significantly changed with time, which reached a peak at 3-5 days postinjury. Several MMP isoforms correlated with markers of secondary injury in female patients (all P < .05). Additionally, serum levels of MMP-3 (P = .011) in males and MMP-10 (P = .044) in females were significantly associated with long-term functional outcomes in a sex-specific manner. CONCLUSIONS: This is the first sex-specific study to examine serum MMP levels and their correlation with clinicoradiologic measures after intracerebral hemorrhage, and identifies potential biomarkers of secondary injury and long-term outcomes in both sexes.


Assuntos
Hemorragia Cerebral/enzimologia , Metaloproteinases da Matriz/sangue , Adulto , Idoso , Biomarcadores/sangue , Hemorragia Cerebral/sangue , Hemorragia Cerebral/complicações , Hemorragia Cerebral/diagnóstico por imagem , Bases de Dados Factuais , Avaliação da Deficiência , Edema/sangue , Edema/enzimologia , Edema/etiologia , Feminino , Escala de Coma de Glasgow , Humanos , Escala de Gravidade do Ferimento , Isoenzimas , Masculino , Pessoa de Meia-Idade , Valor Preditivo dos Testes , Prognóstico , Fatores de Risco , Fatores Sexuais , Fatores de Tempo , Tomografia Computadorizada por Raios X
9.
Mediators Inflamm ; 2019: 1936769, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30833826

RESUMO

Cold and hot thermal therapies are widely used as a traditional therapy in many cultures and are often prescribed in the treatment of various musculoskeletal and neurological conditions which present themselves to primary care physicians. However, there are no reports that investigated either the effects of cold and hot thermal therapies on the skin inflammation of trimellitic anhydride- (TMA-) induced dermatitis-like contact hypersensitivity (CHS) mouse model, or the mechanism of thermal therapy on allergic skin inflammation. Therefore, in this study, to reveal the anti-inflammatory effect of thermal therapy and its mechanism on TMA-induced CHS, we analyzed ear-swelling response (ear edema), vascular permeability, serum IgE levels, histological examination, and histamine and Th2 cytokine levels. Cold thermal therapy reduced the ear-swelling response, the vascular permeability, the serum IgE levels, and the infiltration of eosinophils and mast cells as well as the mast cell degranulation. To determine the mechanism by which cold thermal therapy inhibits allergic skin inflammation, detailed studies were carried out revealing that cold thermal therapy suppressed IL-4 and IL-5 secretion and mast cell activation. These results indicated that cold thermal therapy cures skin inflammation of TMA-induced CHS by decreasing Th2 cytokine release, especially IL-4 and IL-5, and mast cell activation. These data suggest that new insight into the mechanism of robust therapeutic effects of cold thermal therapy against allergic dermatitis, and cold thermal therapy may prove to be a useful therapeutic modality on allergic inflammatory diseases as traditional use as well as Th2- or mast cell-mediated allergic responses.


Assuntos
Dermatite Atópica/induzido quimicamente , Dermatite Atópica/terapia , Anidridos Ftálicos/toxicidade , Animais , Dermatite Atópica/sangue , Otopatias/sangue , Otopatias/induzido quimicamente , Otopatias/terapia , Edema/sangue , Edema/induzido quimicamente , Edema/terapia , Histamina/sangue , Imunoglobulina E/sangue , Interleucina-4/sangue , Interleucina-5/sangue , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Anafilaxia Cutânea Passiva , Distribuição Aleatória , Células Th2/metabolismo
10.
Psychopharmacology (Berl) ; 236(6): 1717-1728, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30607480

RESUMO

RATIONALE: Glucagon-like peptide-1 (GLP-1) and glucagon-like peptide-2 (GLP-2) are gut derived hormones. GLP-1 and GLP-2 were shown to have pleiotropic effects in intestinal and pancreatic diseases. OBJECTIVE: We aimed to investigate the activities of GLP-1 and GLP-2 on nociception and inflammation in mice, involving their actions on serotonergic, nitrergic, and opioidergic systems. METHODS: Antinociceptive and anti-inflammatory activities of intraperitoneally injected GLPs were evaluated in hotplate latency test, formalin-induced behavioral, and paw edema tests. Ondansetron, a selective 5-HT3 receptor antagonist; L-NAME, a NOS inhibitor; and naloxone, an opioid antagonist were injected to determine the mechanisms of antinociception and anti-inflammation. We also measured blood glucose levels and performed rotarod test in order to evaluate whether the hypoglycemic effect of GLP compounds or alterations in locomotor activity may affect the latency in hotplate test and activity in formalin test. RESULTS: GLP-1 (0.2 mg/kg) and GLP-2 (0.05, 0.2 mg/kg) significantly increased pain threshold. GLP-1 (0.2 mg/kg) and GLP-2 (0.05, 0.1, 0.2 mg/kg) significantly decreased formalin-induced licking and shaking behaviors. GLP-1 or GLP-2 showed no significant inhibitory action on formalin-induced swelling in paws of mice. Antinociceptive actions of GLP-1 and GLP-2 were significantly decreased with ondansetron and naloxone, and paw shaking behavior significantly increased with naloxone. GLP-1 and GLP-2 did not impair rotarod performance, and did not cause a significant hypoglycemic effect in our normoglycemic mice after rotarod test. CONCLUSION: These finding indicated that the antinociceptive and anti-inflammatory effect of GLP-1 was related to opioidergic system. Antinociceptive effect of GLP-2 was partially related to 5-HT3 serotonergic or opioidergic system in hotplate test. However, the anti-inflammatory effect of GLP-2 was not directly related to 5-HT3, NO or opioids.


Assuntos
Analgésicos/farmacologia , Anti-Inflamatórios/farmacologia , Peptídeos Semelhantes ao Glucagon/farmacologia , Antagonistas de Entorpecentes/farmacologia , Óxido Nítrico/metabolismo , Receptores 5-HT3 de Serotonina/metabolismo , Analgésicos/uso terapêutico , Animais , Anti-Inflamatórios/uso terapêutico , Relação Dose-Resposta a Droga , Avaliação Pré-Clínica de Medicamentos/métodos , Edema/sangue , Edema/tratamento farmacológico , Edema/patologia , Feminino , Peptídeos Semelhantes ao Glucagon/uso terapêutico , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Naloxona/farmacologia , Nociceptividade/efeitos dos fármacos , Nociceptividade/fisiologia , Dor/sangue , Dor/tratamento farmacológico , Dor/patologia , Medição da Dor/efeitos dos fármacos , Medição da Dor/métodos , Extratos Vegetais/farmacologia , Teste de Desempenho do Rota-Rod/métodos , Antagonistas do Receptor 5-HT3 de Serotonina/farmacologia
11.
Rev Recent Clin Trials ; 14(1): 50-55, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30387401

RESUMO

BACKGROUND: Blood cytokines affect the development of inflammatory processes in both normal and pathological states. We have studied changes in the concentration of interleukins (ILs) - 1ß, IL-4, IL-10, IL-12B p40, transforming growth factor ß (TGF ß), tumor necrosis factor (TNF-α) in acute carrageenan-induced inflammation and degenerative-dystrophic changes of knee joint caused by monoiodoacetate-induced Osteoarthritis (OA) in experimental models on rats. We also investigated the change in the cytokine profile during prophylactic and therapeutic administration of chondroitin sulfate to animals under experimental conditions. METHODS: The concentration of the cytokines was measured in blood serum by enzyme-linked immunosorbent assay. RESULTS: The manifestation of articular lesions was characterized by a disturbance in the balance between proinflammatory (IL-1ß, IL-12B p40, TNF-α) and anti-inflammatory (IL-4, IL-10, TGF -ß) cytokines. CONCLUSION: A reduction in the concentration of proinflammatory cytokines in blood serum after prophylactic and therapeutic administration of chondroitin sulfate to the rat with experimental models of acute inflammation of the hind limb and degenerative-dystrophic changes in the knee joint with OA is associated with anti-inflammatory and regenerative properties of the drug.


Assuntos
Sulfatos de Condroitina/administração & dosagem , Citocinas/sangue , Citocinas/efeitos dos fármacos , Edema/tratamento farmacológico , Osteoartrite/tratamento farmacológico , Animais , Artrite Experimental/sangue , Artrite Experimental/tratamento farmacológico , Carragenina/farmacologia , Modelos Animais de Doenças , Edema/sangue , Edema/induzido quimicamente , Ácido Iodoacético/farmacologia , Masculino , Osteoartrite/induzido quimicamente , Distribuição Aleatória , Ratos , Ratos Wistar , Sensibilidade e Especificidade
12.
BMJ Case Rep ; 11(1)2018 Dec 22.
Artigo em Inglês | MEDLINE | ID: mdl-30580305

RESUMO

Eosinophilic fasciitis (EF) is a syndrome of unknown aetiology characterised by progressive collagenous thickening of the subcutaneous fascia. Limb oedema can precede the skin thickening and induration classically associated with EF. We describe a case of EF in a 31-year-old woman who presented to her general practitioner with lower limb oedema and stiffness. Blood tests in primary care showed a persistently raised alanine transferase (ALT). No hepatic cause for her raised ALT was found despite investigation. The unusual manner of her presentation led to delay in her referral to the autoimmune connective tissue disease (CTD) clinic. This case illustrates the importance of considering autoimmune CTD such as EF in young patients presenting with limb oedema and raised ALT, as early treatment influences prognosis and functional recovery.


Assuntos
Alanina Transaminase/sangue , Doenças Autoimunes/sangue , Edema/sangue , Eosinofilia/sangue , Fasciite/sangue , Adulto , Doenças Autoimunes/diagnóstico , Doenças Autoimunes/patologia , Diagnóstico Diferencial , Edema/etiologia , Edema/patologia , Eosinofilia/complicações , Eosinofilia/patologia , Fasciite/complicações , Fasciite/patologia , Feminino , Humanos , Extremidade Inferior/patologia
13.
Medicine (Baltimore) ; 97(51): e13733, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30572513

RESUMO

INTRODUCTION: To assess the association between growth differentiation factor-15 (GDF15) and radiographic features including bone marrow edema and bone erosion in Spondyloarthritis (SpA). METHODS: Patients with SpA (n = 120) receiving treatment in the Guangdong General Hospital, China, between August 2012 and December 2016 were retrospectively included. Serum of patients and healthy controls (n = 30) were collected and GDF15 levels were measured using ELISA. Inflammation was assessed by C-reactive protein (CRP), and magnetic resonance imaging (MRI) of the sacroiliac joint using Spondyloarthritis Research Consortium of Canada score and a method of dichotomy to assess fat metaplasia, bone erosion, and ankylosis. Radiographs of the pelvis were scored using the modified New York (mNY) score. RESULTS: Serum GDF15 levels were higher in SpA patients compared to controls (503.52 ±â€Š222.92 vs. 190.86 ±â€Š104.18 pg/mL, P < .0001). Patients who suffered from bone erosion on MRI had higher levels of GDF15 (525.72 [186.33, 801.62]vs. 428.06 [255.15, 670.98] pg/mL, P = .0375). There was a positive correlation between serum GDF15 and CRP (r = 0.5442, P < .0001). Moreover, GDF15 levels were related to CRP levels (r = 0.5658, P < .0001) in those X-ray scores were III, according to 1984mNY criteria. Receiver operating characteristic (ROC) analysis showed that GDF15 levels above 501.98pg/mL could predict presence of bone erosion on MRI. CONCLUSION: The present study suggested that serum GDF15 levels are higher in SpA patients than in healthy controls. The GDF15 level was correlated with CRP and may be a surrogate biomarker in bone erosion.


Assuntos
Reabsorção Óssea/sangue , Reabsorção Óssea/etiologia , Fator 15 de Diferenciação de Crescimento/sangue , Espondilartrite/sangue , Espondilartrite/complicações , Adolescente , Adulto , Biomarcadores/sangue , Doenças da Medula Óssea/sangue , Doenças da Medula Óssea/diagnóstico por imagem , Doenças da Medula Óssea/etiologia , Reabsorção Óssea/diagnóstico por imagem , Proteína C-Reativa/metabolismo , Edema/sangue , Edema/diagnóstico por imagem , Edema/etiologia , Feminino , Humanos , Imagem por Ressonância Magnética , Masculino , Projetos Piloto , Estudos Retrospectivos , Espondilartrite/diagnóstico por imagem , Adulto Jovem
14.
Biomed Pharmacother ; 108: 309-315, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30227323

RESUMO

Smilax glabra Roxb. (Tufuling) and Bolbostemma paniculatum (Maxim.) Franquet (Tubeimu) are used as couplet medicine in traditional Chinese medicine for the treatment of arthritis. This study is conducted to provide evidence on their therapeutic effects on rheumatoid arthritis (RA) and to explore its possible mechanisms of action. The identification and quantification of representative components (Astilbin and Tubeimoside I) in the n-butyl alcohol fraction of this couplet medicine (BFCM) were carried out by HPLC-UV assays. The contents of Astilbin and Tubeimoside I in BFCM were 13.13% (15.434 min) and 3.4% (18.619 min) respectively. For the assessment of anti-RA and anti-inflammatory activities, a carrageenan-induced paw edema model in rats was used. The swelling rates of paws and levels of IL-1ß, IL-6 and TNF-α in the swelling tissue were determined. We observed that the BFCM exhibited significant inhibitory activity on carrageenan-induced paw edema model (p<0.01). The down regulated levels of IL-1ß, IL-6 and TNF-α (all p<0.05) were reported. The results indicate that BFCM possesses significant anti-RA and anti-inflammatory effects, and it has a potential to be developed as a new therapeutic agent against RA.


Assuntos
Artrite Reumatoide/tratamento farmacológico , Cucurbitaceae/química , Edema/tratamento farmacológico , Extratos Vegetais/uso terapêutico , Smilax/química , Animais , Artrite Reumatoide/sangue , Artrite Reumatoide/complicações , Artrite Reumatoide/patologia , Peso Corporal , Carragenina , Citocinas/metabolismo , Modelos Animais de Doenças , Edema/sangue , Edema/complicações , Edema/patologia , Mediadores da Inflamação/metabolismo , Rim/efeitos dos fármacos , Rim/patologia , Rim/fisiopatologia , Fígado/efeitos dos fármacos , Fígado/patologia , Fígado/fisiopatologia , Masculino , Modelos Biológicos , Especificidade de Órgãos , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Ratos Sprague-Dawley
15.
Molecules ; 23(8)2018 Aug 09.
Artigo em Inglês | MEDLINE | ID: mdl-30096961

RESUMO

Periploca forrestii Schltr. (P. forrestii) is a species used in Traditional Chinese Medicine (TCM) known as "Miao medicine", and has a long history of use in the treatment of rheumatism, rheumatoid arthritis (RA), and joint pain. The present study aimed to evaluate the anti-arthritis effects of the cardenolide-rich and caffeoylquinic acid-rich fractions (CDLFs and CQAFs) of P. forrestii in collagen-induced arthritic (CIA) rats, and defined the mechanisms of therapeutic action in MH7A cells treated with TNF-α. Serum rheumatoid factor (RF), TNF-α, IL-6, IL-1ß, PGE2, NO, SOD, and MDA were determined by ELISA or other commercially assay kits. Histopathological changes in ankle joint tissues were examined. The mRNA expressions of IL-1ß, IL-6, COX-2, and iNOS in MH7A cells were measured by qRT-PCR assays. In addition, the expressions of iNOS, COX-2, and p65 proteins, and the phosphorylation of IκBα, p38, ERK1/2, and JNK proteins in MH7A cells were analyzed by Western blot. The results showed that CDLF and CQAF could suppress the paw swelling in CIA rats at different doses (125 mg/kg, 250 mg/kg, and 500 mg/kg). Histopathological examination suggests that the CDLF and CQAF significantly relieved the damage of the structure of the ankle joint in CIA rats. In addition, serum RF, TNF-α, IL-6, IL-1ß, PGE2, NO, and MDA were decreased, along with increased activity of serum SOD. Furthermore, CDLF and CQAF downregulated the expressions of IL-1ß, IL-6, COX-2, iNOS, and p65, and inhibited the phosphorylation of IκBα, p38, ERK1/2, and JNK in MH7A cells treated with TNF-α. These findings demonstrated that both CDLF and CQAF exhibited anti-arthritic activity, which might be associated with their inhibitory effects on the NF-κB and MAPK signaling pathways.


Assuntos
Artrite Reumatoide/tratamento farmacológico , Cardenolídeos/química , Periploca/química , Ácido Quínico/análogos & derivados , Animais , Artrite Reumatoide/sangue , Artrite Reumatoide/complicações , Linhagem Celular , Ciclo-Oxigenase 2/metabolismo , Citocinas/sangue , Edema/sangue , Edema/tratamento farmacológico , Edema/patologia , Humanos , Proteínas I-kappa B/metabolismo , Inflamação/complicações , Inflamação/tratamento farmacológico , Inflamação/patologia , Sistema de Sinalização das MAP Quinases/efeitos dos fármacos , Masculino , Malondialdeído/metabolismo , Óxido Nítrico/sangue , Óxido Nítrico Sintase Tipo II/metabolismo , Tamanho do Órgão , Fosforilação/efeitos dos fármacos , Ácido Quínico/farmacologia , Ácido Quínico/uso terapêutico , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Ratos Sprague-Dawley , Superóxido Dismutase/metabolismo , Fator de Transcrição RelA/metabolismo
16.
J Vasc Surg Venous Lymphat Disord ; 6(4): 500-510, 2018 07.
Artigo em Inglês | MEDLINE | ID: mdl-29909855

RESUMO

OBJECTIVE: We aimed to evaluate the effects of intermittent pneumatic compression (IPC) in patients at low mobility with leg edema. METHODS: A pilot, two-arm, randomized controlled clinical trial was performed. Fifty patients (age, 58.4 ± 9 years; male, 14), randomly allocated to a group (IPC) undergoing 1 month (n = 29) of an in-home cycle of IPC and to a control (C) group (n = 21), were studied. Leg edema was evaluated by measuring subcutaneous thickness (high-resolution ultrasound) and circumferences (metric tape), both assessed at different levels of the lower limbs, and volume (water plethysmography). Ankle range of motion (ROM, goniometer), quality of life (QoL) by the 36-Item Short Form Health Survey, and a pool of plasma inflammatory markers were also evaluated. RESULTS: Edema significantly decreased in the IPC group (for all outcome measures, P < .0001), whereas it significantly increased in the C group (P < .0001). Ankle ROM was significantly enhanced in the IPC group (dorsiflexion, P < .0001; plantar flexion, P = .002) and remained stable in the C group. QoL showed an improvement in the IPC group, particularly significant for the general health subscale (P = .004), whereas no changes were highlighted in the C group. The two groups exhibited different trends and variations for some plasma inflammatory markers, mainly for granulocyte colony-stimulating factor. CONCLUSIONS: In a sample of patients at reduced mobility with leg edema, IPC treatment was effective in reducing the edema, improving the ankle ROM, and determining a positive impact on QoL together with a slight modulation of some plasma inflammatory markers.


Assuntos
Edema/terapia , Dispositivos de Compressão Pneumática Intermitente , Extremidade Inferior/irrigação sanguínea , Limitação da Mobilidade , Idoso , Articulação do Tornozelo/fisiopatologia , Antropometria , Artrometria Articular , Biomarcadores/sangue , Edema/sangue , Edema/diagnóstico , Edema/fisiopatologia , Feminino , Humanos , Mediadores da Inflamação/sangue , Masculino , Pessoa de Meia-Idade , Projetos Piloto , Pletismografia , Qualidade de Vida , Amplitude de Movimento Articular , Recuperação de Função Fisiológica , Método Simples-Cego , Inquéritos e Questionários , Fatores de Tempo , Resultado do Tratamento , Ultrassonografia
17.
Biomed Pharmacother ; 97: 1710-1721, 2018 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-29793335

RESUMO

Ziziphora clinopodioides has been used in traditional medicine for its anti-inflammatory properties. Current study is believed to first time report the potential of Z. clinopodioides extracts to ameliorate joint inflammation using model of chronic joint inflammation (FCA-induced rheumatoid arthritis). The study further investigates the effects on joint inflammation using acute inflammatory paw edema models. The anti-inflammatory effects were also supported by using xylene-induced ear edema model. Results showed that Z. clinopodioides significantly ameliorated rheumatoid arthritis as indicated by the inhibition of arthritic development and paw edema. Histopathological examination showed significant attenuation in pannus formation, bone erosion, and joint inflammation. Treatment with the plant extracts also nearly normalized counts of RBCs, platelets, and total leukocytes along with hemoglobin (Hb) content. Biochemical analysis (AST, ALT, urea, and creatinine) showed that plant extracts did not possess hepatotoxic or nephrotoxic effects. Water displacement plethysmometric analysis showed that Z. clinopodioides significantly attenuated carrageenan-induced paw edema. To evaluate the mechanism, anti-inflammatory effects were further evaluated using histamine- and serotonin-induced inflammatory paw edema models. Z. clinopodioides significantly suppressed paw edema induced by both histamine and serotonin, and also caused the inhibition of xylene-induced ear edema. This suggested the inhibition of autacoids as one of the mechanisms of anti-inflammatory effects of plant. GC-MS analysis showed that the plant is rich in essential oils, including terpenoids, esters, alcohols, furans, cyclic ketones, epoxides, oxanes, and acyclic hydrocarbons. In conclusion, current study demonstrated that Z. clinopodioides possessed significant anti-arthritic and anti-inflammatory properties which might be attributed to the inhibition of autacoids.


Assuntos
Artrite Reumatoide/tratamento farmacológico , Edema/tratamento farmacológico , Inflamação/tratamento farmacológico , Lamiaceae/química , Extratos Vegetais/uso terapêutico , Doença Aguda , Animais , Artrite Experimental/sangue , Artrite Experimental/complicações , Artrite Experimental/tratamento farmacológico , Artrite Experimental/patologia , Artrite Reumatoide/sangue , Artrite Reumatoide/complicações , Artrite Reumatoide/patologia , Carragenina , Doença Crônica , Edema/sangue , Edema/complicações , Edema/patologia , Feminino , Hemoglobinas/metabolismo , Histamina , Inflamação/sangue , Inflamação/complicações , Inflamação/patologia , Masculino , Camundongos Endogâmicos BALB C , Compostos Fitoquímicos/análise , Extratos Vegetais/farmacologia , Ratos Sprague-Dawley , Xilenos
18.
BMC Infect Dis ; 18(1): 174, 2018 04 13.
Artigo em Inglês | MEDLINE | ID: mdl-29653519

RESUMO

BACKGROUND: Remitting seronegative symmetrical synovitis with pitting edema (RS3PE) is a rare syndrome characterized by "remitting," "seronegative" (namely rheumatoid factor-negative), and "symmetrical" synovitis with pitting edema on the dorsum of the hands and feet. Recently, there have been reports that serum vascular endothelial growth factor (VEGF) is elevated in this condition. CASE PRESENTATION: An 85-year-old man visited our department with a rash that had appeared 2 days earlier and a fever that had developed on the day of his visit. Based on clinical findings of fever, erythema exudativum multiforme, transitory hypotension, conjunctiva hyperemia, elevated creatine kinase, and desquamation, we suspected toxic shock syndrome (TSS). Therefore, we started treatment with vancomycin (1 g/day) and clindamycin (600 mg/day), after which his fever rapidly remitted. However, pitting edema on the dorsum of his hands and feet appeared on day 7, and the patient also had painful wrist and ankle joints. Additional tests were negative for rheumatoid factor, and anti-cyclic citrullinated protein antibodies were < 0.2 U/mL. Further, serum matrix metalloproteinase-3 (199.6 ng/mL; reference value ≤123.8 ng/mL) and serum VEGF (191 pg/mL; reference value ≤38.3 pg/mL) levels were elevated, and human leukocyte antigen-A2 was detected. The patient was thus diagnosed with RS3PE syndrome, for which he satisfied all four diagnostic criteria: 1) pitting edema in the limbs, 2) acute onset, 3) age ≥ 50 years, and 4) rheumatoid factor negativity. He was treated with oral prednisolone, resulting in the normalization of his serum VEGF level to 34.5 pg/mL 1 month after starting treatment. It is currently 1 year since disease onset, and although the patient has stopped taking prednisolone, there has been no recurrence of RS3PE syndrome. CONCLUSIONS: To the best of our knowledge, this is the first reported case of a patient developing RS3PE syndrome during the clinical course of TSS. We propose that the onset mechanism involved an increase in blood VEGF due to TSS, which induced RS3PE syndrome. As serum VEGF becomes elevated with both severe infections associated with shock and RS3PE syndrome, awareness that these conditions can occur concurrently is essential.


Assuntos
Choque Séptico/complicações , Sinovite/complicações , Idoso de 80 Anos ou mais , Edema/sangue , Edema/complicações , Edema/patologia , Mãos , Humanos , Masculino , Fator Reumatoide/sangue , Choque Séptico/sangue , Choque Séptico/patologia , Síndrome , Sinovite/sangue , Sinovite/patologia , Fator A de Crescimento do Endotélio Vascular/sangue
19.
J Appl Toxicol ; 38(10): 1274-1281, 2018 10.
Artigo em Inglês | MEDLINE | ID: mdl-29603306

RESUMO

Epidemic dropsy is a potentially life-threatening condition resulting from the ingestion of argemone oil derived from the seeds of Argemone mexicana Linn. Exposure to argemone oil is usually inadvertent, arising from mustard cooking oil adulteration. Sanguinarine, an alkaloid present in argemone oil, has been postulated as a causative agent with the severity of epidemic dropsy correlating with plasma sanguinarine levels. Cases of epidemic dropsy have also been reported following the topical application of argemone containing massage oil. Black salve, a topical skin cancer therapy also contains sanguinarine, but at significantly higher concentrations than that reported for contaminated massage oil. Although not reported to date, a theoretical risk therefore exists of black salve inducing epidemic dropsy. This literature review explores the presentation and pathophysiology of epidemic dropsy and assesses the risk of it being induced by black salve.


Assuntos
Argemone/química , Benzofenantridinas/toxicidade , Edema/induzido quimicamente , Isoquinolinas/toxicidade , Óleos Vegetais/toxicidade , Preparações de Plantas/toxicidade , Animais , Benzofenantridinas/sangue , Benzofenantridinas/isolamento & purificação , Edema/sangue , Humanos , Isoquinolinas/sangue , Isoquinolinas/isolamento & purificação , Óleos Vegetais/química , Sementes/química
20.
Microvasc Res ; 117: 61-73, 2018 05.
Artigo em Inglês | MEDLINE | ID: mdl-29407525

RESUMO

Type 2 diabetes mellitus (DM2) is frequently accompanied by microcirculation complications, including structural and functional alterations, which may have serious effects on substance exchanges between blood and interstitial tissue and the health of organs. In this paper, we aim to study the influence of microcirculation alterations in DM2 patients on fluid and oxygen exchanges through a model analysis. A fluid flow and oxygen transport model were developed by considering the interplay between blood in capillary network and interstitial tissue. The two regions were separately represented by 1D network model and 3D volume model, and the immersed boundary method (IBM) was adopted to solve fluid and mass transfer between these two regions. By using the model, the steady flow field and the distributions of oxygen in capillary network and surrounding tissue were firstly simulated. In the interstitial volume, fluid pressure and oxygen tension decreased with the increase of distance from the network; in the network, oxygen tension in blood plasma dropped from 100 mm Hg at the entrance to about 40 mm Hg at the exit. We further tested several structural and functional disorders related to diabetic pathological conditions. Simulated results show that the impaired connectivity of the network could result in poor robustness in maintaining blood flow and perfused surface; under high fluid permeability conditions of capillary walls, the pressure gradient was much larger around the capillary bed, and this alteration led to a saturation level of the interstitial pressure when lymphatic flow drainage can't work effectively; the variations in network connectivity and permeability of capillary wall also had unfavorable influence on oxygen distributions in interstitial tissue. In addition, when the oxygen releasing capacity of hemoglobin was confined by glycosylated hemoglobin (HbA1) in the case of diabetes, the plasma could not be complemented with adequate oxygen and thus the hypoxic tissue range will be extended. This study illustrates that when microcirculation disturbances, including the structure of capillary network, the wall osmosis property and the capacity of blood binding oxygen occur in DM2, some negative impacts are raised on microvascular hemodynamics and metabolism circumstance of interstitial tissue.


Assuntos
Capilares/fisiopatologia , Simulação por Computador , Diabetes Mellitus Tipo 2/complicações , Angiopatias Diabéticas/etiologia , Microcirculação , Modelos Cardiovasculares , Oxigênio/sangue , Capilares/metabolismo , Capilares/patologia , Diabetes Mellitus Tipo 2/sangue , Diabetes Mellitus Tipo 2/fisiopatologia , Angiopatias Diabéticas/sangue , Angiopatias Diabéticas/fisiopatologia , Edema/sangue , Edema/etiologia , Edema/fisiopatologia , Hemoglobina A Glicada/metabolismo , Hemodinâmica , Humanos , Isquemia/sangue , Isquemia/etiologia , Isquemia/fisiopatologia , Análise Numérica Assistida por Computador , Osmose , Oxiemoglobinas/metabolismo
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA