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1.
Urologe A ; 59(3): 347-358, 2020 Mar.
Artigo em Alemão | MEDLINE | ID: mdl-32072200

RESUMO

In recent years new surgical techniques to treat benign prostatic hyperplasia have been introduced into urological practice and evaluated in clinical studies. Complications of standard procedures, e.g. transurethral resection of the prostate (TURP), can be avoided while maintaining equivalent clinical outcomes. The main goal is preservation of erectile and ejaculatory function. Furthermore, outpatient treatment associated with a rapid patient recovery would be desirable. This article presents the course of the interventions, the mechanisms of action and current clinical evidence for novel mechanical approaches of recanalization, water-based ablation as well as prostate artery embolization. Initial study results partially indicate that in the future practically all patients can be offered an individualized surgical technique, which provides optimal symptomatic and functional improvements with a minimized risk of complications.


Assuntos
Ejaculação , Ereção Peniana , Hiperplasia Prostática/cirurgia , Ressecção Transuretral da Próstata/métodos , Ejaculação/fisiologia , Humanos , Masculino , Tratamentos com Preservação do Órgão , Ereção Peniana/fisiologia , Resultado do Tratamento
2.
Int Braz J Urol ; 45(6): 1216-1226, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31808411

RESUMO

INTRODUCTION AND OBJECTIVE: Several studies have focused on the treatment and recurrence of urethral diverticulum (UD). However, few investigations have addressed sexual function in pati-ents with UD. Therefore, we sought to examine sexual function in women affected by UD. MATERIALS AND METHODS: There were 108 accepted cases involving transvaginal diverticulectomy at our institution. Ultimately, 83 women were included for further analysis, only 61 of these wo-men had sexual partners. We collected data for the Female Sexual Function Index (FSFI) from the female patients and the Male Sexual Health Questionnaire (MSHQ) from their male partners before and after surgery. RESULTS: Preoperatively, the UD size affected the female patient's arousal and lubrication (p=0.04), and the UD location affected their satisfaction. However, no significant between-group differences were found in the total FSFI score. For all women, sexual activity improved after surgery (p=0.0087). In addition to improvements in arousal for women with a large UD, improvements in lubrication were affected by the UD size, number and shape, increases in sa-tisfaction scores were impacted by the UD location and shape, and pain relief was linked to the UD number and shape. Analysis of the MSHQ results revealed no between-group differences among the male partners. CONCLUSION: Only the UD size and location affected sexual function in women with a small UD. Surgery could improve female sexual function but did not affect the sexual function of the patient's partners.


Assuntos
Divertículo/cirurgia , Complicações Pós-Operatórias/etiologia , Disfunções Sexuais Fisiológicas/etiologia , Doenças Uretrais/cirurgia , Adulto , Divertículo/fisiopatologia , Ejaculação/fisiologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Satisfação do Paciente , Complicações Pós-Operatórias/fisiopatologia , Valores de Referência , Estudos Retrospectivos , Fatores de Risco , Fatores Sexuais , Disfunções Sexuais Fisiológicas/fisiopatologia , Parceiros Sexuais , Inquéritos e Questionários , Doenças Uretrais/fisiopatologia
4.
Anim Reprod Sci ; 207: 107-117, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31204090

RESUMO

Nerve growth factor-ß (NGF) is a seminal plasma protein associated with improved sperm membrane integrity and motility in mammalian species. The objective of this study was to compare post-thaw semen quality from both ejaculated and pididymal-collected bull sperm incubated with purified NGF prior to cryopreservation. Semen was obtained from Angus × Simmental crossbred bulls (n = 10) collected by electroejaculation, followed by castration and epididymal sperm collections 3 days later. Semen samples were incubated with extender having 0 ng/mL (CONT), 0.5 ng/mL (LOW), 5 ng/mL (MED), or 50 ng/mL (HIGH) of purified NGF prior to cryopreservation. Sperm motility was assessed in each sample prior to treatment and cryopreservation and at post-thaw. Flow cytometry was used for post-thaw assessment of sperm viability (SYBR-14/PI), acrosome integrity (FITC-PNA/PI), and chromatin stability (acridine orange). Values for post-thaw sperm motility and velocity variables were decreased, while linearity was increased in samples of the HIGH compared with CONT group (P < 0.01), but there were no differences in epididymal samples (P> 0.05). Samples from the HIGH group also had a lesser amplitude of lateral head displacement at 2.5 and 3 h post-thaw (P < 0.01). Post-thaw sperm viability, acrosome integrity, and DNA fragmentation index were not affected by NGF treatment in either ejaculated or epididymal sperm (P> 0.05). In conclusion, supplementation of freezing extender with NGF had minimal effects on post-thaw sperm quality in bulls. Results indicate NGF may have a function in preventing premature sperm hyperactivation in ejaculated, but not epididymal-collected spermatozoa. Fertility studies, both in vitro and in vivo, are warranted to ascertain the relevancy of these findings.


Assuntos
Bovinos , Criopreservação , Crioprotetores/farmacologia , Fator de Crescimento Neural/farmacologia , Preservação do Sêmen , Sêmen/efeitos dos fármacos , Animais , Criopreservação/métodos , Criopreservação/veterinária , Crioprotetores/administração & dosagem , Combinação de Medicamentos , Ejaculação/fisiologia , Estimulação Elétrica , Congelamento/efeitos adversos , Masculino , Fator de Crescimento Neural/administração & dosagem , Sêmen/fisiologia , Análise do Sêmen/veterinária , Preservação do Sêmen/métodos , Preservação do Sêmen/veterinária , Recuperação Espermática
5.
Anim Reprod Sci ; 205: 1-9, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30962039

RESUMO

There are different perspectives on whether there should be use of electroejaculation (EE) for semen collection because it can be stressful and painful for the males when this technique is imposed. In the present review it is examined 1) the effects of EE on animal welfare and semen quality in domestic and wild small ruminants, 2) benefits and limitations of administering anaesthetics and sedatives prior to EE, 3) advantages/disadvantages of transrectal ultrasonic-guided massage of the accessory sex glands (TUMASG) as an alternative to EE, and 4) benefits of administering hormones, such as oxytocin or PGF2α analogues (which stimulate the contractility of the male accessory sex glands), prior to EE and TUMASG. In general, the administration of anaesthetics, sedatives or hormones reduces the pain and stress caused by EE, and can improve sperm quality, but results may vary depending on the species. The use of anaesthetics is, however, not devoid of risks and pre-EE administration of sedatives, or oxytocin or PGF2α analogues, can aid sperm collection mitigate risks. The TUMASG is less stressful than EE, but its effectiveness varies greatly among species, and it can only be performed by trained personnel. Prior administration of the hormones may also result in a reduction in the period needed to induce ejaculation with use of TUMASG procedures.


Assuntos
Bem-Estar do Animal , Ejaculação/fisiologia , Estimulação Elétrica , Ruminantes , Análise do Sêmen/veterinária , Animais , Masculino
6.
Arch Ital Urol Androl ; 91(1): 35-42, 2019 Mar 29.
Artigo em Inglês | MEDLINE | ID: mdl-30932427

RESUMO

The objective of this study was to search for an alternative technique to relieve prostatic obstruction due to benign prostatic hyperplasia without affecting the ejaculatory function. The technical requirements are a laser with a wavelength well absorbed by water (good vaporizing effect) and at the same time by hemoglobin (good hemostatic effect) and laser fibers very resistant at high emission power allowing perfect vaporization in a contact mode. The aim of the technique is to avoid damage of the structures that allow the peripheral region of the prostate and the seminal vesicles to discharge their secretions into the posterior urethra. The orifices of the ejaculatory ducts must therefore be identified and preserved, damage of the ejaculatory ducts along their path inside the prostate must be avoided and the so called "genital sphincter" must be saved. The steps of the Leonardi Ejaculation Sparing Technique (LEST) procedure are as follow: Step 1 - ejaculatory duct orifices must be identified and the limits of the vaporization section must be marked. Step 2 - bladder neck is cleaned of the prostate hypertrophic tissue saving, as much as possible, the smooth muscle fibers of the bladder neck. Step 3 - vapo-resection of the lateral lobe (or enucleation of the adenoma) is performed. Step 4 - cautious and meticulous preparation of the prostatic apexes is obtained with saving of the orifices of the ejaculatory ducts. An antegrade ejaculation is maintained in about 80% of cases in patients without a middle lobe, although in the presence of a middle lobe this rate drops to about 50%.


Assuntos
Ejaculação/fisiologia , Terapia a Laser/métodos , Próstata/cirurgia , Hiperplasia Prostática/cirurgia , Humanos , Masculino , Próstata/patologia , Hiperplasia Prostática/patologia , Bexiga Urinária/cirurgia
7.
Reprod Fertil Dev ; 31(5): 1009-1016, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30908956

RESUMO

Various studies have sought to determine the typical v. optimal abstinence period after which semen samples should be collected, with many contradictory results reported. Several factors influence the semen microenvironment, and thus sperm parameters. In this study we focused on the secretions of the prostate, seminal vesicles and the epididymis. Semen samples were obtained from healthy normozoospermic males (n=16) after 4-day and 4-h periods of ejaculatory abstinence, and standard semen analysis was performed using computer-aided sperm analysis, whereas seminal plasma citric acid, neutral α-glucosidase and fructose concentrations were measured using assay kits. There were significant decreases in total sperm count (P<0.001), sperm concentration (P<0.05) and semen volume (P<0.05) after 4h compared with 4 days ejaculatory abstinence. Furthermore, increases were observed in total sperm motility (P<0.05) and sperm progressive motility (P<0.01) after a 4-h abstinence period, accompanied by significant reductions in citric acid (P<0.05), α-glucosidase (P<0.01) and fructose (P<0.01) concentrations. In addition, due to the decreased number of spermatozoa, these concentrations translated to a significant decrease in fructose (P<0.05) per spermatozoon, indicating an intrinsic mechanism capitalising on alternative sources of energy for increased metabolic function and subsequent sperm motility.


Assuntos
Ejaculação/fisiologia , Motilidade Espermática/fisiologia , Adulto , Ácido Cítrico/análise , Frutose/análise , Humanos , Masculino , Sêmen/química , Análise do Sêmen , Contagem de Espermatozoides , Adulto Jovem , alfa-Glucosidases/análise
8.
Reproduction ; 157(4): R109-R126, 2019 04 01.
Artigo em Inglês | MEDLINE | ID: mdl-30668523

RESUMO

Despite serving the primary objective of ensuring that at least one sperm cell reaches and fertilises an ovum, the male ejaculate (i.e. spermatozoa and seminal fluid) is a compositionally complex 'trait' that can respond phenotypically to subtle changes in conditions. In particular, recent research has shown that environmentally and genetically induced changes to ejaculates can have implications for offspring traits that are independent of the DNA sequence encoded into the sperm's haploid genome. In this review, we compile evidence from several disciplines and numerous taxonomic systems to reveal the extent of such ejaculate-mediated paternal effects (EMPEs). We consider a number of environmental and genetic factors that have been shown to impact offspring phenotypes via ejaculates, and where possible, we highlight the putative mechanistic pathways by which ejaculates can act as conduits for paternal effects. We also highlight how females themselves can influence EMPEs, and in some cases, how maternally derived sources of variance may confound attempts to test for EMPEs. Finally, we consider a range of putative evolutionary implications of EMPEs and suggest a number of potentially useful approaches for exploring these further. Overall, our review confirms that EMPEs are both widespread and varied in their effects, although studies reporting their evolutionary effects are still in their infancy.


Assuntos
Evolução Biológica , Ejaculação/fisiologia , Herança Paterna , Espermatozoides/fisiologia , Animais , Feminino , Masculino
9.
Medicine (Baltimore) ; 98(3): e14109, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30653133

RESUMO

To explore a novel surgical treatment for primary premature ejaculation using an inner condom technique.A total of 20 males with premature ejaculation, who admitted our andrology clinic from June 2016 to July 2017, were enrolled. By surgery, an inner condom made of acellular dermal matrix (ADM) was transferred to the subcutaneous pocket of the penis. The prolongation of intravaginal ejaculatory latency time (IELT) after the surgery was examined. The perioperative complications were also studied.The surgical intervention significantly increased the average IELT in patients, from 0.67 to 2.37 min (P = .009). No serious perioperative complications and adverse psychosexual effects were seen. Patients could resume sexual activity 6 weeks after the surgery.The novel inner condom using ADM is an effective and safe surgical treatment for males with premature ejaculation. The efficacy of this new treatment modality warrants further investigation in independent cohorts.


Assuntos
Preservativos , Ejaculação Precoce/cirurgia , Procedimentos Cirúrgicos Urológicos Masculinos/instrumentação , Adolescente , Adulto , Ejaculação/fisiologia , Desenho de Equipamento , Humanos , Masculino , Pessoa de Meia-Idade , Ejaculação Precoce/fisiopatologia , Comportamento Sexual/fisiologia , Fatores de Tempo , Resultado do Tratamento , Procedimentos Cirúrgicos Urológicos Masculinos/métodos , Adulto Jovem
10.
Andrologia ; 51(1): e13146, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30255511

RESUMO

Maintaining sperm motility after ejaculation is important for fertilisation. Apoptosis may play an important role to reduce sperm motility after ejaculation. The aim of this study was to perceive whether or not an increase in apoptosis reduces sperm motility in a higher degree after ejaculation and whether it can be predicted by laboratory tests, such as sperm chromatin structure assay (SCSA). Fifty-one Asthenozoospermia and 20 fertile subjects participated in this study. SCSA was applied using flow cytometry. Fluorescein-labelled inhibitors of Caspases (FLICA) method was used for assessment of active Caspase-3. Motility was assessed every 2 hr after ejaculation for 12 hr. Both SCSA and spermatozoa with active Caspase-3 were significantly correlated with the rate of motility reduction after ejaculation. In the subgroups who had SCSA <27% and active Caspase-3 <40%, the sperm motility reduction significantly occurred 6-8 hr after ejaculation compared to the fresh sample. In the cases of SCSA ≥27% and active Caspase-3 ≥ 40%, a significant decrease in motility was observed between 2 and 4 hr after ejaculation. The result demonstrated a significant trend in the rate of sperm motility reduction with SCSA increase, which suggests SCSA may indirectly show a good scheme of apoptosis status and may forecast the rate of motility reduction after ejaculation in Asthenozoospermia.


Assuntos
Astenozoospermia/fisiopatologia , Cromatina/metabolismo , Motilidade Espermática/fisiologia , Espermatozoides/fisiologia , Adulto , Apoptose/fisiologia , Astenozoospermia/metabolismo , Caspase 3/metabolismo , Dano ao DNA/fisiologia , Fragmentação do DNA , Ejaculação/fisiologia , Humanos , Masculino , Espermatozoides/metabolismo , Adulto Jovem
11.
Urology ; 124: 254-256, 2019 02.
Artigo em Inglês | MEDLINE | ID: mdl-30366047

RESUMO

Due to the rarity of the disease, adverse events related to ejaculatory function following the management of paratesticular rhabdomyosarcoma with multimodal therapy in adolescents are rarely discussed. Two patients, age 14 and 15 at time of diagnosis were treated with multimodal therapy with nerve-sparing retroperitoneal lymph node dissection, chemotherapy, and radiotherapy. Each developed ejaculatory dysfunction during the treatment period, which resolved 1 year after completion of all therapies. We sought to assess the role of each component of multimodal therapy on the observed side effect and the potential for delayed recovery of function after cessation of all therapies.


Assuntos
Ejaculação/fisiologia , Excisão de Linfonodo , Rabdomiossarcoma/cirurgia , Neoplasias Testiculares/cirurgia , Adolescente , Quimiorradioterapia Adjuvante , Humanos , Excisão de Linfonodo/métodos , Masculino , Recuperação de Função Fisiológica , Espaço Retroperitoneal , Fatores de Tempo
12.
Sex Med Rev ; 7(1): 129-140, 2019 01.
Artigo em Inglês | MEDLINE | ID: mdl-30057136

RESUMO

INTRODUCTION: Besides erectile dysfunction, premature ejaculation (PE) is the most frequent male sexual disorder and shows a high level of bother and distress. The negative consequences of long-term and unsuccessfully treated PE on both patient and partner are well established in the literature and include personal distress, impairment of the partner's sexual function, and interpersonal difficulties. AIM: To outline the consequences of untreated PE and the advantages and disadvantages of currently available treatment options with a special focus on a new topical eutectic lidocaine/prilocaine metered dose spray (Fortacin; Lidocaine/Prilocaine, Recordati, Milan, Italy) which represents the second officially approved drug in this indication. METHODS: Narrative overview of the literature synthesizing the findings of literature retrieved from searches of computerized databases such as Web of Science, Medline, PubMed, and Google Scholar, hand searches, and authoritative texts. Combinations of keywords including premature ejaculation, ejaculatory control, intravaginal ejaculation latency time, IELT, PE, PDE5 inhibitors (PDE5i), SSRIs, topical anesthetics, lidocaine, prilocaine, and treatment were used. In the end, 59 studies published between 2000 and 2018 were considered relevant for this review. MAIN OUTCOME MEASURES: Published studies on PE-related negative psychosocial outcomes, as well as advantages and disadvantages of currently available off-label and officially approved treatment options. RESULTS: Although a variety of treatment options for PE have shown marked improvements in stopwatch-measured intravaginal ejaculation latency time (IELT) and patient-reported outcomes as assessed by the Premature Ejaculation Profile (PEP), none of the investigated drugs has reached market approval. The only so far officially approved medication-dapoxetine-is characterized by high discontinuation rates of up to 90%, mostly because of high side effects, cost issues, efficacy below expectations, and the need for scheduling sexual intercourse. CONCLUSION: With the official approval in Europe the new dose-metered lidocaine-prilocaine spray (Fortacin) may become a real first-line therapy option for PE and may offer a satisfactory and affordable solution, especially because of its unique galenic preparation, making its handling easy and customer friendly. In addition, it has the potential to significantly increase the currently low patients' acceptance of available monotherapies and become an established second-line therapy for the severe PE patients with IELTs <1 to 2 minutes or with ante-portal ejaculation in combination with oral therapy. Porst H, Burri A. Novel treatment for premature ejaculation in the light of currently used therapies: A review. Sex Med Rev 2019; 7:129-140.


Assuntos
Benzilaminas/uso terapêutico , Ejaculação/efeitos dos fármacos , Naftalenos/uso terapêutico , Ereção Peniana/efeitos dos fármacos , Inibidores da Fosfodiesterase 5/uso terapêutico , Ejaculação Precoce/tratamento farmacológico , Coito , Ejaculação/fisiologia , Humanos , Masculino , Satisfação do Paciente , Ereção Peniana/psicologia , Ejaculação Precoce/fisiopatologia , Ejaculação Precoce/psicologia , Ensaios Clínicos Controlados Aleatórios como Assunto , Resultado do Tratamento
13.
J Sex Med ; 15(11): 1600-1608, 2018 11.
Artigo em Inglês | MEDLINE | ID: mdl-30274957

RESUMO

INTRODUCTION: Although links between ejaculatory control or intravaginal ejaculatory latency time and female sexual functioning have frequently been reported in the past, no study has investigated the importance of other male ejaculatory characteristics, such as ejaculation volume and intensity, for women's sexuality. AIM: To assess the importance of subjectively perceived ejaculation intensity and ejaculation volume for female sexual function and satisfaction. METHODS: This was a cross-sectional online survey including 240 sexually active, heterosexual women (median age 27.4 years), using study-specific questions and validated questionnaires. MAIN OUTCOME MEASURE: Results are presented as means, percentages, and age-controlled partial correlation coefficients of the main study variables. RESULTS: 50.43% of women considered it very important that the partner ejaculates during intercourse. 18.3% of women preferred that the partner ejaculates before they reach orgasm, whereas for 53.5% this did not matter. 22.6% of women stated that they experienced a more intense orgasm when their partner ejaculated during vaginal intercourse. 17.4% reported that they definitely experienced a more intensive orgasm depending on the intensity of their partner's ejaculation, whereas for 17.8% this did not matter at all. 20.9% of women did not feel that their orgasm was more intense depending on the subjectively felt ejaculate quantity, whereas the majority (37.9%) stated that it did not matter. 13.1% of women regarded the quantity of expelled ejaculate as an expression of their own sexual attractiveness. Women stating that they experienced more intense orgasms when the partner ejaculated, when the partner experienced a more intense ejaculation, and when he expelled a greater ejaculate quantity also reported better lifelong orgasmic function (r = 0.24, r = 0.15, r = .26, respectively) and more lifelong sexual satisfaction (r = .29, r = .15, r = 26, respectively). CLINICAL IMPLICATIONS: The perception of ejaculatory characteristics can be related to the female partner's sexual satisfaction and overall sexual functioning. STRENGTH & LIMITATIONS: This is the very first study to explore the importance of male ejaculation volume and intensity for women's sexual functioning. Data are of self-report nature and ejaculation characteristics were not objectively measured but by women's self-report. CONCLUSION: Although male ejaculation and its different aspects seem to play an important role for women, the study demonstrates a considerable variability of women's attitudes toward ejaculatory characteristics. Further research is required to examine the sources of this variability. Burri A, Buchmeier J, Porst H. The importance of male ejaculation for female sexual satisfaction and function. J Sex Med 2018;15:1600-1608.


Assuntos
Ejaculação/fisiologia , Orgasmo/fisiologia , Satisfação Pessoal , Adulto , Estudos Transversais , Feminino , Humanos , Internet , Masculino , Pessoa de Meia-Idade , Inquéritos e Questionários , Suíça , Adulto Jovem
14.
Zhonghua Nan Ke Xue ; 24(4): 360-363, 2018 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-30168959

RESUMO

Seminal vesicles are involved in semen accumulation in the process of ejaculation, contracting and releasing seminal vesicle fluid accounting for about 50-80% of the semen, and the fructose in their secretions is an indispensable nutrient for sperm maturation. Thus, seminal vesicles are important male accessary glands closely related with the quality and quantity of sperm. In the process of semen accumulation, sympathetic and parasympathetic nerves participate in the regulation of the secretory function of seminal vesicle epithelia and the contraction of the smooth muscle layer as well as the distribution of adrenonergic, cholinergic, dopaminergic and various neurotransmitter receptors in the seminal vesicle epithelia and smooth muscle layer, which play a significant role in male fertility. This review discusses the neurophysiological effects of seminal vesicles in ejaculation.


Assuntos
Ejaculação/fisiologia , Sêmen/fisiologia , Glândulas Seminais/fisiologia , Animais , Masculino , Análise do Sêmen , Espermatozoides
15.
Learn Mem ; 25(10): 513-521, 2018 10.
Artigo em Inglês | MEDLINE | ID: mdl-30224554

RESUMO

Early experiences with sexual reward play a pivotal role in the formation of sexual behavior and partner preference. Associations of salient partner cues, or even neutral cues on a partner, with sexual reward states are a product of Pavlovian learning. However, the extent to which first experiences that associate a neutral stimulus with no immediate consequence, and how that association may affect subsequent associability after being paired with a sexual reward state after copulation to ejaculation, remains unclear. To address this question, sexually naïve males were preexposed over one or five trials to almond scented gauze pads prior to training during which half of the males were trained 10 times with scented receptive females, and the other half with unscented receptive females. A final test of partner preference was conducted in a large open field containing two sexually receptive females, one scented and the other unscented. Males developed a conditioned ejaculatory preference for the type of female they were trained with, except when they were preexposed five times to the odor and then trained with females bearing the same odor, indicating a significant CS preexposure effect. One CS preexposure was not sufficient to inhibit subsequent conditioning. Exposure to the scent before perfusion for inmunohistochemistry, revealed different patterns of brain activation in brain areas previously associated with the development of partner preference, like the medial preoptic area, ventral tegmental area, nucleus accumbens, basolateral amygdala, among others, depending on group membership. Thus, CS preexposure results in a subsequent impairment of the association that links the odor cue to sexual reward and preference. This highlights the impact of the first sexual experiences in future partner preference.


Assuntos
Encéfalo/fisiologia , Ejaculação/fisiologia , Comportamento Sexual Animal/fisiologia , Animais , Feminino , Imuno-Histoquímica , Masculino , Percepção Olfatória/fisiologia , Proteínas Proto-Oncogênicas c-fos/metabolismo , Distribuição Aleatória , Ratos Long-Evans , Recompensa
16.
Learn Mem ; 25(10): 522-532, 2018 10.
Artigo em Inglês | MEDLINE | ID: mdl-30224555

RESUMO

We have shown previously that male rats develop a conditioned ejaculatory preference (CEP) for females scented with a neutral odor like almond or lemon that is paired with the male's post-ejaculatory reward state during their first and subsequent early sexual experiences. However, preexposing males to the neutral odor alone prior to its pairing with sexual reward results in latent inhibition. Here, we examined the phenomenon of unconditioned stimulus (US) preexposure, in which male rats were preexposed to the ejaculatory reward state either one or five times with scented (ScF) versus unscented (UnScF) females prior to multiple ejaculatory trials with females in the opposite condition (e.g., ScF preexposure received 10 subsequent ejaculatory trials with UnScF, whereas UnScF preexposure received 10 subsequent ejaculatory trials with ScF). As before, mate and partner preference was evaluated in an open field where each male had access to two females, one ScF and the other UnScF. Males that underwent five trials of preexposure did not display a CEP for either female. Conversely, males preexposed once to a ScF, and later trained with UnScF developed a preference for the latter, whereas males preexposed once to the UnScF, and then trained with ScF did not show a preference for any of the females. Subsequent exposure to the odor cue alone revealed different patterns of brain activation in areas related to sexual behavior that depended on the animal's group membership. Altogether, these findings demonstrate the pivotal role of first sexual experiences in the establishment of future sexual partner preference in the male rat, and suggest an innate preference for estrous odors over neutral odors that can become conditioned subsequently as predictors of sexual reward.


Assuntos
Ejaculação , Comportamento Sexual Animal , Animais , Encéfalo/metabolismo , Ejaculação/fisiologia , Feminino , Masculino , Percepção Olfatória/fisiologia , Proteínas Proto-Oncogênicas c-fos/metabolismo , Distribuição Aleatória , Ratos Long-Evans , Recompensa , Comportamento Sexual Animal/fisiologia
17.
Reprod Domest Anim ; 53 Suppl 2: 70-73, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30238660

RESUMO

Local vessels ultrasonography evaluates prostatic physio-pathologic states. Testosterone promotes tissue and vascular growth. Knowing variables on prostatic vasculature is crucial to correctly apply Pulsed-Wave exam. The study aims to assess how ejaculation and blood testosterone affect Pulsed-Wave indexes. Serial blood testosterone dosages and Pulsed-Wave exams were performed in 20 dogs, immediately before (T0) and after (T1) ejaculation and 6 hr later (T2). Arteria prostatica cranialis, Arteriola capsularis, Arteriola trabecularis and Arteriola parenchimalis were evaluated and mean Pulsatility and Resistivity Index, Systolic-Peak, End-Diastolic and Mean Velocity calculated. Data were grouped by time and vessel (ANOVA, p ≤ 0.05). At T1, Resistivity Index significantly lowered in A. prostatica cranialis, A. trabecularis and A. parenchimalis but grew in A. capsularis; Pulsatility Index had the same pattern, but not significant in A. parenchimalis; Systolic Peak Velocity, End-Diastolic Velocity, Mean Velocity significantly rose in A. capsularis and A. trabecularis. No indexes differed at T0 and T2. Testosterone did not differ at T0 (10.93 ± 7.05 ng/ml), T1 (12.71 ± 7.29) and T2 (10.54 ± 6.63). Results stated the risen prostatic vascular flow postejaculation, affecting Pulsed-Wave. Due to semi-rigid capsule, impairing vasodilation of other vessels, only A. capsularis indexes increased. Intimal cushions of A. prostatica cranialis kept velocities fixed; A. capsularis and A. trabecularis lack of intimal cushions, thus velocities grew. In A. parenchimalis, precapillary sphincters opening allows increased flow redistribution in vasodilated parenchymal bed, keeping velocities fixed. As testosterone, not affected by ejaculation, did not peak, vascular changes are not due to testosterone itself. These physiological effects of ejaculation suggest proper sexual rest before Pulsed-Wave exam planned to explore suspected prostatic neovascularization.


Assuntos
Cães/fisiologia , Ejaculação/fisiologia , Próstata/diagnóstico por imagem , Próstata/fisiologia , Testosterona/sangue , Animais , Velocidade do Fluxo Sanguíneo , Diástole , Masculino , Sístole , Ultrassonografia Doppler/veterinária
18.
Neurosci Lett ; 685: 155-159, 2018 10 15.
Artigo em Inglês | MEDLINE | ID: mdl-30170041

RESUMO

The medial preoptic area (MPN) plays an important role in the control of male sexual behavior. In rats, the central part of the MPN (MPNc) is sexually dimorphic and contains a sexually dimorphic nucleus composed of neurons expressing calbindin-D28 K (CALB-SDN). Although the functions of the MPNc are not well understood, surgical destruction of the MPNc adversely affects the performance of sexual behavior in sexually naive males, but not in sexually experienced males, supporting the notion that the MPNc changes functionally with sexual experience. In this study, we aimed to determine the effects of sexual experience on the neuronal activity of the MPNc and CALB-SDN. Sexual behavior in sexually inexperienced males that had no experience of ejaculation, and experienced males that had ejaculated once was observed. After they displayed sexual behavior, the brains were sampled, and immunohistochemical analysis of c-Fos, a neuronal activity marker, in the MPNc and CALB-SDN was performed. The numbers of c-Fos-immunopositive cells with or without calbindin-D28K-immunoreactivity increased significantly in the MPNc and CALB-SDN following ejaculation in both sexually inexperienced and experienced males, although the numbers did not change significantly with exposure to estrous female odors, the first mount, and the first intromission before and after the first ejaculation. We further found that the number of c-Fos-immunopositive and calbindin-D28K-immunonegative cells in the MPNc, but not in the CALB-SDN, was significantly smaller in sexually experienced males than in sexually inexperienced males. These results suggest that a population of MPNc neurons, which is located outside the CALB-SDN and do not express calbindin-D28 K, are activated during the first copulation and then silent after acquisition of sexual experience.


Assuntos
Neurônios/metabolismo , Área Pré-Óptica/metabolismo , Comportamento Sexual Animal/fisiologia , Comportamento Sexual/fisiologia , Animais , Ejaculação/fisiologia , Masculino , Proteínas Proto-Oncogênicas c-fos/metabolismo , Caracteres Sexuais
19.
Reprod Domest Anim ; 53(6): 1555-1562, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30085374

RESUMO

Male reproductive parameters are often used for the functional examination and evaluation of predicted genetic values for future aspects. However, these traits are relatively reliable until the measurable effects are expressed on desired traits. Therefore, we aimed to associate the single nucleotide polymorphism (SNP) genotype of the investigated characteristics and reproductive loci. A total of 46 male dogs are divided into three age groups (I ≤ 3 years, n = 19; II 4-6 years; n = 19, and III ≥7 years, n = 8). The testis, scrotum and body weight, libido sexualis and ejaculation time for each fraction were monitored as functional traits, while the pH, fractional semen volume, motility, concentration, and abnormal and dead spermatozoa rate were recorded as spermatological traits. The Affymetrix Canine 127 K SNP genotyping array v2 (Affymetrix Inc., California, USA) was used for SNP genotyping. In the primary results, the scrotal circumference was found to be higher in group II compared to other groups (p < 0.05) and the lowest total abnormal spermatozoa rate was found in group I (p < 0.05). The normal spermatozoa rate was found to be significantly above the threshold in relation to the SNP in chromosome 17. In conclusion, this study represents an exciting first step towards SNP association with dog semen spermatological parameters. Future studies might be undertaken to evaluate this SNP region for gene-knockout and expression analysis and for fine mapping to validate and/or discover the exact position of the effect region.


Assuntos
Cães/genética , Reprodução/genética , Espermatozoides/fisiologia , Fatores Etários , Animais , Ejaculação/fisiologia , Estudo de Associação Genômica Ampla , Libido/fisiologia , Masculino , Polimorfismo de Nucleotídeo Único , Escroto/anatomia & histologia , Análise do Sêmen/veterinária , Motilidade Espermática/genética , Testículo/anatomia & histologia
20.
Rev Int Androl ; 16(1): 42-44, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30063023

RESUMO

Postorgasmic illness syndrome (POIS) is a rarely described syndrome characterized by transient flu-like symptoms and cognition disorders. Recent studies suggest that immunogenic reactivity to autologous semen is the underlying mechanism in POIS. Our study is regarding a 30-year-old that visited our unit for an allergy consultation because he experienced malaise after ejaculations. Skin prick tests and intracutaneous tests with autologous diluted semen with negative results were performed. Immunoblotting and western blot of the patient's autologous semen showed negative results. To complete the study, we intended to rule out other possible causes such as urological, hormonal, or neuropsychiatric disorders. We present a case of POIS based on the clinical criteria that did not show an IgE mediated cause. In the case of our patient, we could not identify the underlying cause; however, we believe that the possible involvement of neurobiochemical mediators should be studied.


Assuntos
Fadiga/etiologia , Sêmen/imunologia , Disfunções Sexuais Psicogênicas/diagnóstico , Adulto , Ejaculação/fisiologia , Humanos , Imunoglobulina E/imunologia , Masculino , Disfunções Sexuais Psicogênicas/imunologia , Síndrome
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