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1.
BMC Cardiovasc Disord ; 23(1): 8, 2023 01 09.
Artigo em Inglês | MEDLINE | ID: mdl-36624380

RESUMO

BACKGROUND: Pulmonary vein isolation (PVI) is recommended to treat paroxysmal and persistent atrial fibrillation (AF). This analysis aimed to assess the hospital efficiency of single-shot cryoballoon ablation (CBA) and point-by-point radiofrequency ablation (RFA). METHODS: The discrete event simulation used PVI procedure times from the FREEZE Cohort study to establish the electrophysiology (EP) lab occupancy time. 1000 EP lab days were simulated according to an illustrative German hospital, including 3 PVI cases per day using CBA at one site and RFA at the other. RESULTS: The analysis included 1560 CBA patients and 1344 RFA patients from the FREEZE Cohort. Some baseline patients' characteristics were different between groups (age, AF type, and some concomitant diseases), without being statistically associated to ablation procedure time. Mean procedure time was 122.2 ± 39.4 min for CBA and 160.3 ± 53.5 min for RFA (p < 0.0001). RFA was associated with a more than five-fold increase of cumulative overtime compared to CBA over the simulated period (1285 h with RFA and 253 h with CBA). 70.7% of RFA lab days included overtime versus 25.7% for CBA. CBA was associated with more days with an additional hour at the end of the EP lab shift compared to RFA (47.8% vs 11.5% days with one hour left, respectively). CONCLUSION: CBA is faster and more predictable than point-by-point RFA, and enables improvements in EP lab efficiency, including: fewer cumulative overtime hours, more days where overtime is avoided and more days with remaining time for the staff or for any EP lab usage. Clinical trial registration NCT01360008 (first registration 25/05/2011).


Assuntos
Fibrilação Atrial , Ablação por Cateter , Criocirurgia , Veias Pulmonares , Humanos , Estudos de Coortes , Criocirurgia/efeitos adversos , Fibrilação Atrial/diagnóstico , Fibrilação Atrial/cirurgia , Ablação por Cateter/efeitos adversos , Ablação por Cateter/métodos , Veias Pulmonares/cirurgia , Eletrofisiologia , Resultado do Tratamento , Recidiva
2.
Int J Cardiovasc Imaging ; 39(1): 145-152, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36598692

RESUMO

Although PVCs commonly lead to degraded cine cardiac MRI (CMR), patients with PVCs may have relatively sharp cine images of both normal and ectopic beats ("double beats") when the rhythm during CMR is ventricular bigeminy, and only one beat of the pair is detected for gating. MRI methods for directly imaging premature ventricular contractions (PVCs) are not yet widely available. Localization of PVC site of origin with images may be helpful in planning ablations. The contraction pattern of the PVCs in bigeminy provides a "natural experiment" for investigating the potential utility of PVC imaging for localization. The purpose of this study was to evaluate the correlation of the visually assessed site of the initial contraction of the ectopic beats with the site of origin found by electroanatomic mapping. Images from 7 of 86 consecutive patients who underwent CMR prior to PVC ablation were found to include clear cine images of bigeminy. The visually apparent site of origin of the ectopic contraction was determined by three experienced, blinded CMR readers and correlated with each other, and with PVC site of origin determined by 3D electroanatomic mapping during catheter ablation. Blinded ascertainment of visually apparent initial contraction pattern for PVC localization was within 2 wall segments of PVC origin by 3D electroanatomic mapping 76% of the time. Our data from patients with PVCs with clear images of the ectopic beats when in bigeminy provide proof-of-concept that CMR ectopic beat contraction patterns analysis may provide a novel method for localizing PVC origin prior to ablation procedures. Direct imaging of PVCs with use of newer cardiac imaging methods, even without the presence of bigeminy, may thus provide valuable data for procedural planning.


Assuntos
Ablação por Cateter , Complexos Ventriculares Prematuros , Humanos , Complexos Ventriculares Prematuros/diagnóstico por imagem , Complexos Ventriculares Prematuros/cirurgia , Valor Preditivo dos Testes , Imageamento por Ressonância Magnética , Imagem Cinética por Ressonância Magnética , Ablação por Cateter/métodos , Eletrofisiologia
3.
Biofabrication ; 15(2)2023 Feb 02.
Artigo em Inglês | MEDLINE | ID: mdl-36652708

RESUMO

Advances in primary and stem cell derived neuronal cell culture techniques and abundance of available neuronal cell types have enabledin vitroneuroscience as a substantial approach to modelin vivoneuronal networks. Survival of the cultured neurons is inevitably dependent on the cell culture incubators to provide stable temperature and humidity and to supply required CO2levels for controlling the pH of culture medium. Therefore, imaging and electrophysiology recordings outside of the incubator are often limited to the short-term experimental sessions. This restricts our understanding of physiological events to the short snapshots of recorded data while the major part of temporal data is neglected. Multiple custom-made and commercially available platforms like integrated on-stage incubators have been designed to enable long-term microscopy. Nevertheless, long-term high-spatiotemporal electrophysiology recordings from developing neuronal networks needs to be addressed. In the present work an incubator-independent polydimethylsiloxane-based double-wall perfusion chamber was designed and integrated with multi-electrode arrays (MEAs) electrophysiology and compartmentalized microfluidic device to continuously record from engineered neuronal networks at sub-cellular resolution. Cell culture media underwent iterations of conditioning to the ambient CO2and adjusting its pH to physiological ranges to retain a stable pH for weeks outside of the incubator. Double-wall perfusion chamber and an integrated air bubble trapper reduced media evaporation and osmolality drifts of the conditioned media for two weeks. Aligned microchannel-microfluidic device on MEA electrodes allowed neurite growth on top of the planar electrodes and amplified their extracellular activity. This enabled continuous sub-cellular resolution imaging and electrophysiology recordings from developing networks and their growing neurites. The on-chip versatile and self-contained system provides long-term, continuous and high spatiotemporal access to the network data and offers a robustin vitroplatform with many potentials to be applied on advanced cell culture systems including organ-on-chip and organoid models.


Assuntos
Incubadoras , Microscopia , Perfusão , Microeletrodos , Eletrofisiologia , Dispositivos Lab-On-A-Chip
4.
Life Sci ; 315: 121356, 2023 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-36621537

RESUMO

INTRODUCTION: The neuropathology of Parkinson's disease (PD) is complex and affects multiple systems of the body beyond the central nervous system. This study examined the effects of gallic acid (GA) and gastrointestinal vagotomy (VG) on motor, cognitive, intestinal transit time, and thalamic nuclei electrical power in an animal model of PD induced by rotenone. MATERIALS AND METHODS: Male Wistar rats were divided into 4 groups: Sham, ROT, ROT+GA, VG + ROT. Sham rats received vehicle, those in ROT received rotenone (5 mg/kg/2 ml, ig), PD rats in ROT+GA were treated with GA (100 mg/kg, gavage/once daily, for 28 days), and in VG + ROT, the vagal nerve was dissected. Stride length, motor coordination and locomotion, intestinal transit time, cognitive and pain threshold, and thalamic local EEG were evaluated. Oxidative stress indexes in striatal tissue were also measured. RESULTS: Rotenone diminished significantly the stride length (p < 0.001), motor coordination (p < 0.001), power of thalamic EEG (p < 0.01) and pain (p < 0.001). MDA increased significantly (p < 0.001) while GPx activity decreased (p < 0.001). Intestinal transit time rose significantly (p < 0.01). PD rats treated with GA improved all above disorders (p < 0.001, p < 0.01). Vagotomy prevented significant alterations of motor and non-motor parameters by rotenone. CONCLUSION: According to current findings, rotenone acts as a toxin in GI and plays a role in the pathogenesis of PD through gastric vagal nerve. Thus, vagotomy could prevent the severity of toxicity by rotenone. In addition, GA improved symptoms of PD induced by rotenone. Therefore, GA can be regarded as a promising therapeutic candidate for PD patients.


Assuntos
Fármacos Neuroprotetores , Doença de Parkinson , Ratos , Masculino , Animais , Rotenona/toxicidade , Ácido Gálico/farmacologia , Ratos Wistar , Doença de Parkinson/patologia , Estresse Oxidativo , Encéfalo , Vagotomia , Eletrofisiologia , Fármacos Neuroprotetores/farmacologia , Modelos Animais de Doenças
5.
ACS Appl Mater Interfaces ; 15(1): 1903-1913, 2023 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-36583722

RESUMO

Here, a strategy to overcome the stiff and brittle nature of cellulose-derived carbon nanofibrils (CCNFs) is proposed through a facile, low-cost, and scalable approach. Flexible and conformal CCNFs with a low bending rigidity below 55.4 mN and tunable conductivities of 0.14-45.5 S m-1 are developed by introducing silanol as a multieffect additive in the electrospun hybrid nanofibrous network and subsequent carbonization at a relatively high temperature (900 °C) and chemical vapor deposition of polypyrrole (PPy) on the hybrid carbon nanofibril surface. Silica acts as a lubricant in each rigid carbon fiber to improve flexibility of the CCNF structure as well as a template during cellulose carbonization to prevent the melting of carbon nanofibrils. Meanwhile, the uniform coating of PPy leads to an improvement in electrical conductivity while conserving the porous structure and compressibility of the CCNF nets. These conductive hybrid CCNF films are evaluated as mechanoreceptors and physiological sensors, which are demonstrated to be applied in intelligent electronics including electronic skin, human-machine interfaces, and epidermic electrodes. The design or working principles of the hybrid CCNFs for achieving optimum applicable effects when applied in different scenarios are revealed.


Assuntos
Celulose , Nanofibras , Humanos , Celulose/química , Polímeros/química , Nanofibras/química , Carbono , Pirróis/química , Condutividade Elétrica , Eletrofisiologia
6.
Rev. esp. cardiol. (Ed. impr.) ; 75(12): 1029-1039, dic. 2022. tab, graf
Artigo em Espanhol | IBECS | ID: ibc-212936

RESUMO

Introducción y objetivos: Se describen los resultados del Registro español de ablación con catéter correspondientes al año 2021, marcado por la recuperación de la actividad tras la pandemia de SARS-CoV-2. Métodos: La recogida de datos fue restrospectiva mediante la cumplimentación y el envío de un formulario específico por los centros participantes. Resultados: Se analizaron los datos de 93 centros (65 públicos, 28 privados). Se comunicaron 17.941 procedimientos de ablación con una media de 193±133 y mediana de 171. La recuperación de la actividad tras el cierre por la pandemia de SARS-CoV-2ha supuesto un marcado aumento de procedimientos (+2.772 casos, +18%) a pesar de un ligero descenso en el número de centros participantes (4 menos que en 2020). La ablación de la fibrilación auricular sigue siendo el procedimiento más frecuente, a distancia del resto de sustratos (5.848; 32,6%). Junto con la ablación del istmo cavotricuspídeo (3.766; 21%) y la taquicardia por reentrada intranodular (3.132; 17,5%), constituye los 3 sustratos abordados con más frecuencia. Las tasas comunicadas de éxito (94%), complicaciones (2%) y mortalidad (0,07%) son similares a las de años previos. Se realizaron 401 procedimientos en pacientes pediátricos (el 3,8% del total). Conclusiones: El Registro español de ablación con catéter recoge de manera sistemática e ininterrumpida la actividad nacional, y este año se ha observado una marcada recuperación de la actividad a pesar del persistente efecto de la pandemia de SARS-CoV-2. La tasa de éxito sigue manteniéndose elevada, con una baja tasa de complicaciones.(AU)


Introduction and objectives: The results of the 2021 Spanish catheter ablation registry are presented. Methods: Data collection was carried out retrospectively by filling in and sending a specific form by the participating centers. Results: Data from 93 centers (65 public, 28 private) were analyzed. A total of 17941 ablation procedures were reported with a mean of 193±133 clases per centre. Recovery of activity from SARS-CoV-2 pandemic lockdown has led to a notable increase in the number of procedures (+2772 procedures, +18%) despite a small decrease in participating centers (4 centers less than in 2020). Atrial fibrillation ablation continues to be the leading procedure, with 5848 procedures (32,6%). Together with ablation of the cavotricuspid isthmus (3766; 21%) and atrioventricular nodal reentrant tachycardia (3132; 17,5%) they constitute the 3 most frequently approached substrates. The total success rate reported (94%) is similar to previous years with a similar rate of complications (2%) and mortality (0.07). A total of 401 procedures were performed in pediatric patients (3,8%). Conclusions: The Spanish catheter ablation registry systematically and continuously collects the national activity, which has recovered significantly from the SARS-CoV-2 pandemic impact in 2020. Success rate for 2021 remains high, with a low complication rate.(AU)


Assuntos
Humanos , Ablação por Cateter , Eletrofisiologia , Arritmias Cardíacas , Vírus da SARS , Infecções por Coronavirus , Pandemias , Cardiologia , Cardiopatias , Estudos Retrospectivos , Inquéritos e Questionários
7.
Metas enferm ; 25(10): 0758-0759, dic. 2022. ilus
Artigo em Espanhol | IBECS | ID: ibc-213288

RESUMO

Elisabet Sánchez Moreno trabaja en el área de electrofisiología de la visión, una especialidad que estudia las respuestas generadas ante estímulos visuales y valora el estado funcional del sistema visual. En 2018 se reconoció como Unidad de Referencia y se formó un equipo de salud específico en el que se incorporó el personal de Enfermería. A continuación nos cuenta todos los detalles.(AU)


Assuntos
Humanos , Feminino , Eletrofisiologia , Testes Visuais , Saúde Ocular , Papel do Profissional de Enfermagem , Enfermagem , Serviços de Enfermagem
8.
Mol Pain ; 18: 17448069221142523, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-36408567

RESUMO

Methylene blue (MB) is an effective treatment for methemoglobinemia, ifosfamide-induced encephalopathy, cyanide poisoning, and refractory vasoplegia. However, clinical case reports and preclinical studies indicate potentially neurotoxic activity of MB at certain concentrations. The exact mechanisms of MB neurotoxicity are not known, and while the effects of MB on neuronal tissue from different brain regions and myenteric ganglia have been examined, its effects on primary afferent neurons from dorsal root ganglia (DRG) have not been studied. Mouse DRG were exposed to MB (0.3-10 µM) in vitro to assess neurite outgrowth. Increasing concentrations of MB (0.3-10 µM) were associated with neurotoxicity as shown by a substantial loss of cells with neurite formation, particularly at 10 µM. In parallel experiments, cultured rat DRG neurons were treated with MB (100 µM) to examine how MB affects electrical membrane properties of small-diameter sensory neurons. MB decreased peak inward and outward current densities, decreased action potential amplitude, overshoot, afterhyperpolarization, increased action potential rise time, and decreased action potential firing in response to current stimulation. MB induced dose-dependent toxicity in peripheral neurons, in vitro. These findings are consistent with studies in brain and myenteric ganglion neurons showing increased neuronal loss and altered membrane electrical properties after MB application. Further research is needed to parse out the toxicity profile for MB to minimize damage to neuronal structures and reduce side effects in clinical settings.


Assuntos
Gânglios Espinais , Azul de Metileno , Ratos , Camundongos , Animais , Azul de Metileno/farmacologia , Azul de Metileno/metabolismo , Gânglios Espinais/metabolismo , Células Receptoras Sensoriais/metabolismo , Eletrofisiologia , Técnicas de Cultura de Células , Células Cultivadas
9.
J Clin Neuromuscul Dis ; 24(2): 61-67, 2022 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-36409335

RESUMO

BACKGROUND: Ulnar nerve is frequently involved in mononeuropathies of the upper limb. Ulnar neuropathies have been diagnosed conventionally using clinical and electrophysiological findings. Physicians opt for nerve imaging in patients with ambiguous electrophysiological tests to gain additional information, identify etiology and plan management. OBJECTIVES: The aim of this study was to describe the electrophysiological and the magnetic resonance neurography (MRN) findings in patients with nontraumatic ulnar neuropathy. METHODS: All consecutive patients with suspected nontraumatic ulnar mononeuropathy were recruited; clinical assessment and electrophysiological studies (EPSs) were done in all. After EPS, patients with localization of lesion along the ulnar nerve underwent MRN. RESULTS: All 39 patients recruited had clinical findings suggestive of ulnar neuropathy; Electrophysiological confirmation was possible in 36/39 (92.30%) patients. Localization of ulnar nerve lesion to elbow and wrist was possible in 27 (75%) and 9 (25%) patients, respectively. MRN was done in 22 patients; a lesion was identified in 19 of 22 (86.36%) ulnar nerves studied. Thickening and hyperintensity in T2 W/short TI inversion recovery images of ulnar nerve at the level of olecranon, suggesting ulnar neuropathy at elbow, was the commonest (8/22) imaging finding. CONCLUSIONS: MRN acts as a complimentary tool to EPS for evaluating nontraumatic ulnar neuropathy. By identifying the etiology, MRN is likely to modify the management decision.


Assuntos
Imageamento por Ressonância Magnética , Neuropatias Ulnares , Humanos , Centros de Atenção Terciária , Espectroscopia de Ressonância Magnética/efeitos adversos , Imageamento por Ressonância Magnética/métodos , Neuropatias Ulnares/diagnóstico por imagem , Eletrofisiologia
10.
Gigascience ; 112022 11 15.
Artigo em Inglês | MEDLINE | ID: mdl-36377463

RESUMO

BACKGROUND: Whole-cell patch-clamp electrophysiology is an essential technique for understanding how single neurons translate their diverse inputs into a functional output. The relative inaccessibility of live human cortical neurons for experimental manipulation has made it difficult to determine the unique features of how human cortical neurons differ from their counterparts in other species. FINDINGS: We present a curated repository of whole-cell patch-clamp recordings from surgically resected human cortical tissue, encompassing 118 neurons from 35 individuals (age range, 21-59 years; 17 male, 18 female). Recorded human cortical neurons derive from layers 2 and 3 (L2&3), deep layer 3 (L3c), or layer 5 (L5) and are annotated with a rich set of subject and experimental metadata. For comparison, we also provide a limited set of comparable recordings from 21-day-old mice (11 cells from 5 mice). All electrophysiological recordings are provided in the Neurodata Without Borders (NWB) format and are available for further analysis via the Distributed Archives for Neurophysiology Data Integration online repository. The associated data conversion code is made publicly available and can help others in converting electrophysiology datasets to the open NWB standard for general reuse. CONCLUSION: These data can be used for novel analyses of biophysical characteristics of human cortical neurons, including in cross-species or cross-lab comparisons or in building computational models of individual human neurons.


Assuntos
Neurônios , Humanos , Masculino , Feminino , Camundongos , Animais , Adulto Jovem , Adulto , Pessoa de Meia-Idade , Técnicas de Patch-Clamp , Neurônios/fisiologia , Eletrofisiologia
11.
Elife ; 112022 11 09.
Artigo em Inglês | MEDLINE | ID: mdl-36350114

RESUMO

Cells convert electrical signals into chemical outputs to facilitate the active transport of information across larger distances. This electrical-to-chemical conversion requires a tightly regulated expression of ion channels. Alterations of ion channel expression provide landmarks of numerous pathological diseases, such as cardiac arrhythmia, epilepsy, or cancer. Although the activity of ion channels can be locally regulated by external light or chemical stimulus, it remains challenging to coordinate the expression of ion channels on extended spatial-temporal scales. Here, we engineered yeast Saccharomyces cerevisiae to read and convert chemical concentrations into a dynamic potassium channel expression. A synthetic dual-feedback circuit controls the expression of engineered potassium channels through phytohormones auxin and salicylate to produce a macroscopically coordinated pulses of the plasma membrane potential. Our study provides a compact experimental model to control electrical activity through gene expression in eukaryotic cell populations setting grounds for various cellular engineering, synthetic biology, and potential therapeutic applications.


Assuntos
Canais Iônicos , Canais de Potássio , Canais Iônicos/genética , Canais Iônicos/metabolismo , Canais de Potássio/metabolismo , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/metabolismo , Fenômenos Fisiológicos Celulares , Eletrofisiologia
12.
Elife ; 112022 11 10.
Artigo em Inglês | MEDLINE | ID: mdl-36355598

RESUMO

A wide range of techniques in neuroscience involve placing individual probes at precise locations in the brain. However, large-scale measurement and manipulation of the brain using such methods have been severely limited by the inability to miniaturize systems for probe positioning. Here, we present a fundamentally new, remote-controlled micropositioning approach composed of novel phase-change material-filled resistive heater micro-grippers arranged in an inchworm motor configuration. The microscopic dimensions, stability, gentle gripping action, individual electronic control, and high packing density of the grippers allow micrometer-precision independent positioning of many arbitrarily shaped probes using a single piezo actuator. This multi-probe single-actuator design significantly reduces the size and weight and allows for potential automation of microdrives. We demonstrate accurate placement of multiple electrodes into the rat hippocampus in vivo in acute and chronic preparations. Our robotic microdrive technology should therefore enable the scaling up of many types of multi-probe applications in neuroscience and other fields.


Assuntos
Neurônios , Procedimentos Cirúrgicos Robóticos , Animais , Ratos , Eletrofisiologia/métodos , Eletrodos Implantados , Encéfalo
13.
J Am Heart Assoc ; 11(22): e026904, 2022 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-36326051

RESUMO

Background There are few US Food and Drug Administration (FDA)-approved devices specifically aimed at the pediatric patient with arrhythmia. This has led to a high off-label utilization of devices in this vulnerable population. The Pediatric and Congenital Electrophysiology Society (PACES), the international organization representing pediatric and congenital heart disease arrhythmia specialists, developed a task force to comprehensively address device development issues relevant to pediatric patients with congenital arrhythmia. Methods and Results As a first step, the taskforce developed a 26-question survey for the pediatric arrhythmia community to assess providers' understanding of the FDA approval process, specifically in regard to pediatric labeling. There were 92/211 respondents (44%) with a >90% completion rate. The vast majority of respondents believed there was a paucity of devices available for children (96%). More than 60% of respondents stated that they did not understand the FDA regulatory process and were not aware of whether the devices they used were labeled for pediatric use. Conclusions Pediatric electrophysiologists are keenly aware of the deficit of available pediatric devices for their patients. The majority do not understand the FDA approval process and could benefit from additional educational resources regarding this. A collaborative forum including PACES, FDA, patients and their families, and Industry would be an important next step in clarifying opportunities and priorities to serve this vulnerable population.


Assuntos
Arritmias Cardíacas , Cardiopatias Congênitas , Humanos , Criança , Estados Unidos , United States Food and Drug Administration , Arritmias Cardíacas/diagnóstico , Arritmias Cardíacas/terapia , Cardiopatias Congênitas/diagnóstico , Cardiopatias Congênitas/terapia , Inquéritos e Questionários , Eletrofisiologia
14.
Biomolecules ; 12(11)2022 11 14.
Artigo em Inglês | MEDLINE | ID: mdl-36421699

RESUMO

This review has examined two of the techniques most used by our research group for evaluating gamete and embryo functionality in animal species, ranging from marine invertebrates to humans. Electrophysiology has given access to fundamental information on some mechanisms underpinning the biology of reproduction. This technique demonstrates the involvement of ion channels in multiple physiological mechanisms, the achievement of homeostasis conditions, and the triggering of profound metabolic modifications, often functioning as amplification signals of cellular communication. Fluorescence spectrometry using fluorescent probes to mark specific cell structures allows detailed information to be obtained on the functional characteristics of the cell populations examined. The simple and rapid execution of this methodology allowed us to establish a panel helpful in elucidating functional features in living cells in a simultaneous and multi-parameter way in order to acquire overall drafting of gamete and embryo functionality.


Assuntos
Corantes Fluorescentes , Células Germinativas , Humanos , Animais , Espectrometria de Fluorescência , Eletrofisiologia
15.
Ann Cardiol Angeiol (Paris) ; 71(5): 294-298, 2022 Nov.
Artigo em Francês | MEDLINE | ID: mdl-36424019

RESUMO

INTRODUCTION: Interventional rhythmology activity (electrophysiology and pacing) has progressed in France during the last decade. The objective of this article was to assess the distribution of this activity depending on the type of centre (public, university or non-university, private). As the French government issued a new regulation regarding rhythmology activity in 2022, the impact of this regulation on activity distribution has been evaluated. METHODOLOGY: All French data activity can be evaluated after accessing to a national database called PMSI [Programme de médicalisation des systèmes d'information]. Based on data collected in year 2019, the level of activity of the different centres, and the impact of the new criteria of the French regulation regarding rhythmology activity have been analysed. RESULTS: About 180,000 arrhythmias interventions have been done in France in 2019 in 377 centres. According to the new criteria of the French regulation, only 76% responded to level A (diagnostic electrophysiology, single- and dual-chamber pacing), 52% for level B (A+ right atrial ablation, implantable automatic defibrillator, cardiac resynchronisation therapy) and 54% for level C (B + left atrial and ventricular ablation). CONCLUSIONS: This work showed that approximately a quarter of the centres do not meet criteria for level A activity, and approximately half of them for levels B and C. The priority will be to reinforce existing centres before allowing new centres to perform arrhythmias management activity in France.


Assuntos
Ablação por Cateter , Desfibriladores Implantáveis , Humanos , Arritmias Cardíacas/terapia , Arritmias Cardíacas/diagnóstico , Bases de Dados Factuais , Eletrofisiologia
16.
Neural Plast ; 2022: 7195699, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36437902

RESUMO

Objective: High-frequency repetitive transcranial magnetic stimulation (HF-rTMS) has been proposed as a promising therapeutic intervention for patients with disorders of consciousness (DOC). However, its therapeutic effects in the literature are inconsistently documented. The primary aim of this study was to explore the alterations in neural connectivity and neurobehavioral reactivity during rTMS modulation in patients with DOC. In addition, safety was investigated as a secondary aim. Methods: The presence of bilateral N20 components in DOC patients was determined by somatosensory-evoked potential (SEP) before enrollment in the study. A total of 64 patients were enrolled and randomly placed into the active and sham groups. Ultimately, 50 patients completed the study. Twenty-five patients in the active group underwent real HF-rTMS, and 25 patients in the sham group underwent sham HF-rTMS, which was delivered over the left dorsolateral prefrontal cortex (DLPFC). The outcome measures of performed pre- and postintervention included the latencies of the N20 and N20-P25 amplitudes of SEP, brainstem auditory-evoked potential (BAEP) grade, JFK Coma Recovery Scale-Revised (CRS-R) score, and Glasgow Coma Scale (GCS) score; any adverse events were recorded at any time during the intervention. Result: Following six weeks of treatment, a significant increase was observed in the total CRS-R and GCS scores, and the N20-P25 amplitudes of patients in the two groups were compared with that obtained from preintervention (all p values < 0.05). The waves of BAEP in the two groups also showed a trend toward normalized activity compared with preintervention grades (p values < 0.05). A significant decrease in the latencies of N20 (p values < 0.001) was observed in the active group compared with measurements obtained from preintervention, whereas no significant decrease was observed in the sham group (p values = 0.013). The improvement in total CRS-R scores (p values = 0.002), total GCS scores (p values = 0.023), and N20-P25 amplitudes (p values = 0.011) as well as the decrease in latencies of N20 (p values = 0.018) and change in BAEP grades (p values = 0.013) were significantly different between the two groups. The parameters in neural connectivity (N20-P25 amplitudes, N20 latencies, and BAEP grades) were significantly correlated with the total CRS-R and GCS scores at postintervention, and the changes of CRS-R before and after interventions have a positive relationship with N20-P25 amplitudes. No adverse events related to the rTMS protocol were recorded. Conclusion: Neural connectivity levels are affected by HF-rTMS and are significantly related to clinical responses in DOC patients with the presence of bilateral N20. The elevation of neural connectivity levels may lay a foundation for successful HF-rTMS treatment for DOC patients.


Assuntos
Estado de Consciência , Estimulação Magnética Transcraniana , Humanos , Eletrofisiologia , Potenciais Somatossensoriais Evocados/fisiologia , Estimulação Magnética Transcraniana/métodos
17.
Int J Mol Sci ; 23(19)2022 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-36232917

RESUMO

Over the last two decades the combination of brain slice patch clamp electrophysiology with optogenetic stimulation has proven to be a powerful approach to analyze the architecture of neural circuits and (experience-dependent) synaptic plasticity in such networks. Using this combination of methods, originally termed channelrhodopsin-assisted circuit mapping (CRACM), a multitude of measures of synaptic functioning can be taken. The current review discusses their rationale, current applications in the field, and their associated caveats. Specifically, the review addresses: (1) How to assess the presence of synaptic connections, both in terms of ionotropic versus metabotropic receptor signaling, and in terms of mono- versus polysynaptic connectivity. (2) How to acquire and interpret measures for synaptic strength and function, like AMPAR/NMDAR, AMPAR rectification, paired-pulse ratio (PPR), coefficient of variance and input-specific quantal sizes. We also address how synaptic modulation by G protein-coupled receptors can be studied with pharmacological approaches and advanced technology. (3) Finally, we elaborate on advances on the use of dual color optogenetics in concurrent investigation of multiple synaptic pathways. Overall, with this review we seek to provide practical insights into the methods used to study neural circuits and synapses, by combining optogenetics and patch-clamp electrophysiology.


Assuntos
Optogenética , Sinapses , Channelrhodopsins , Eletrofisiologia/métodos , Optogenética/métodos , Técnicas de Patch-Clamp , Sinapses/fisiologia , Transmissão Sináptica
18.
J Neural Eng ; 19(5)2022 10 10.
Artigo em Inglês | MEDLINE | ID: mdl-36214526

RESUMO

Objective.Biomedical instrumentation and clinical systems for electrophysiology rely on electrodes and wires for sensing and transmission of bioelectric signals. However, this electronic approach constrains bandwidth, signal conditioning circuit designs, and the number of channels in invasive or miniature devices. This paper demonstrates an alternative approach using light to sense and transmit the electrophysiological signals.Approach.We develop a sensing, passive, fluorophore-free optrode based on the birefringence property of liquid crystals (LCs) operating at the microscale.Main results.We show that these optrodes can have the appropriate linearity (µ± s.d.: 99.4 ± 0.5%,n = 11 devices), relative responsivity (µ± s.d.: 57 ± 12%V-1,n = 5 devices), and bandwidth (µ± s.d.: 11.1 ± 0.7 kHz,n = 7 devices) for transducing electrophysiology signals into the optical domain. We report capture of rabbit cardiac sinoatrial electrograms and stimulus-evoked compound action potentials from the rabbit sciatic nerve. We also demonstrate miniaturisation potential by fabricating multi-optrode arrays, by developing a process that automatically matches each transducer element area with that of its corresponding biological interface.Significance.Our method of employing LCs to convert bioelectric signals into the optical domain will pave the way for the deployment of high-bandwidth optical telecommunications techniques in ultra-miniature clinical diagnostic and research laboratory neural and cardiac interfaces.


Assuntos
Cristais Líquidos , Potenciais de Ação/fisiologia , Animais , Fenômenos Eletrofisiológicos , Eletrofisiologia/métodos , Cristais Líquidos/química , Coelhos , Transdutores
19.
J Exp Clin Cancer Res ; 41(1): 298, 2022 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-36221111

RESUMO

BACKGROUND: The introduction of functional in-silico models, in addition to in-vivo tumor models, opens up new and unlimited possibilities in cancer research and drug development. The world's first digital twin of the A549 cell's electrophysiology in the human lung adenocarcinoma, unveiled in 2021, enables the investigation and evaluation of new research hypotheses about modulating the function of ion channels in the cell membrane, which are important for better understanding cancer development and progression, as well as for developing new drugs and predicting treatments. MAIN BODY: The developed A549 in-silico model allows virtual simulations of the cell's rhythmic oscillation of the membrane potential, which can trigger the transition between cell cycle phases. It is able to predict the promotion or interruption of cell cycle progression provoked by targeted activation and inactivation of ion channels, resulting in abnormal hyper- or depolarization of the membrane potential, a potential key signal for the known cancer hallmarks. For example, model simulations of blockade of transient receptor potential cation channels (TRPC6), which are highly expressed during S-G2/M transition, result in a strong hyperpolarization of the cell's membrane potential that can suppress or bypass the depolarization required for the S-G2/M transition, allowing for possible cell cycle arrest and inhibition of mitosis. All simulated research hypotheses could be verified by experimental studies. SHORT CONCLUSION: Functional, non-phenomenological digital twins, ranging from single cells to cell-cell interactions to 3D tissue models, open new avenues for modern cancer research through "dry lab" approaches that optimally complement established in-vivo and in-vitro methods.


Assuntos
Adenocarcinoma de Pulmão , Neoplasias Pulmonares , Divisão Celular , Eletrofisiologia , Humanos , Neoplasias Pulmonares/patologia , Canal de Cátion TRPC6
20.
Neurosci Biobehav Rev ; 142: 104909, 2022 11.
Artigo em Inglês | MEDLINE | ID: mdl-36228927

RESUMO

Isolated Rapid Eye Movement Sleep (REM) behaviour disorder (iRBD) is a prodromal sign of neurodegenerative disorders. Empirical findings point to a role of non-REM (NREM) sleep alterations in neurodegenerative processes. Therefore, the interest in NREM sleep electroencephalography (EEG) of iRBD is progressively increasing. The present review aims to provide an updated state of the art on NREM sleep electrophysiology in iRBD. First, we describe findings on NREM EEG power spectra. Then, we consider specific NREM sleep EEG hallmarks (i.e., slow waves, slow oscillations, K-complexes, sleep spindles). Finally, we focus on NREM sleep instability. The reviewed literature is small and heterogeneous, but rapidly growing. The most consistent findings point to alteration of sleep spindles and cyclic alternating pattern in RBD. A larger discrepancy characterized results on slow wave activity, but recent studies using a topographical approach provide promising results. Evidence on the relationship of NREM sleep alterations with neurodegenerative processes in iRBD, as well as longitudinal changes, are scarce. We discuss the main methodological limitations, highlighting possible future directions.


Assuntos
Transtorno do Comportamento do Sono REM , Humanos , Eletroencefalografia , Sono/fisiologia , Eletrofisiologia
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