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1.
Annu Int Conf IEEE Eng Med Biol Soc ; 2020: 2221-2226, 2020 07.
Artigo em Inglês | MEDLINE | ID: mdl-33018449

RESUMO

Single-cell dielectrophoretic movement and dielectrophoretic deformation of monocyte cells were interrogated applying 20 Vpp, 50 kHz to 1 MHz signal in the 3D carbon electrode array. Heterogeneity of the monocyte population is shown in terms of the crossover frequencies, translational movement, and deformation index of the cells. The results presented that crossover range for monocytes was 100 kHz - 200 kHz, the translational movement of the cells was rapidly altered when the initial positions of the cells were in the negative dielectrophoretic region. Finally, the deformation index of the monocyte population varied from 0.5 to 1.5.


Assuntos
Carbono , Monócitos , Eletrodos , Eletroforese
3.
J Chromatogr A ; 1626: 461262, 2020 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-32797810

RESUMO

In this paper, a semi-analytical investigation was performed to study the effect of the geometrical parameters of insulator-based dielectrophoresis (iDEP) systems for cell free DNA (cfDNA) trapping. For this purpose, first electrical potential and fluid flow fields were calculated by solving the governing equations including Poisson and Navier-stokes equations with appropriate boundary conditions (BCs) and then a Lagrangian approach was utilized to analyze the motion of cfDNA under the most important forces affected on it including Brownian, Drag, electrophoresis and dielectrophoresis (DEP) forces. The effect of the different parameters such as the electrical conductivity of the medium, shape and geometrical parameters of the insulators on the dielectrophoretic behavior of cfDNA was studied and the optimal value of these parameters was presented. Finally, in order to predict the minimum voltage required for cfDNA trapping, artificial neural network (ANN) was utilized and a relation between input and output parameters was introduced.


Assuntos
Ácidos Nucleicos Livres/química , Eletroforese/métodos , Nanopartículas/química , Condutividade Elétrica , Técnicas Analíticas Microfluídicas , Redes Neurais de Computação
4.
J Chromatogr A ; 1623: 461151, 2020 Jul 19.
Artigo em Inglês | MEDLINE | ID: mdl-32505271

RESUMO

Deterministic Lateral Displacement (DLD) is a microfluidic technique where arrays of micropillars within a microchannel deflect particles leading to size-based segregation. We recently demonstrated that applying AC electric fields orthogonal to the fluid flow increases the separation capabilities of these devices with a deflection angle that depends on the electric field magnitude and frequency. Particle deviation occurs in two distinct regimes depending on frequency. At high frequencies particles deviate due to negative dielectrophoresis (DEP). At low frequencies (below 1 kHz) particles oscillate perpendicular to the flow direction due to electrophoresis and are also deflected within the device. Significantly, the threshold electric field magnitude for the low frequency deviation is much lower than for deflection at high frequencies by DEP. In order to characterize the enhanced separation at low frequencies, the induced deviation was compared between the two frequency ranges. For high frequencies, we develop both theoretically and experimentally scaling laws for the dependence of particle deviation on several parameters, namely the amplitude of the applied voltage, particle size and liquid velocity where DEP forces compete with viscous drag. A novel theoretical framework is presented that enables simulation of particle trajectories subjected to DEP forces in DLD devices. Deviation angles predicted by simulations are in very good agreement with experimental data. At low frequencies (below 1 kHz), particles follow the same scaling law, but with much lower voltages. This indicates that electrokinetic phenomena other than DEP play an important role in driving particle behaviour. Experiments show that at low frequencies, particle motion is affected by quadrupolar electrohydrodynamic flows around the insulating pillars of the DLD array. We quantify the difference between the two frequency regimes and show that an electrokinetic model based only on DEP forces is limited to frequencies of 1 kHz and above.


Assuntos
Simulação por Computador , Eletroforese/métodos , Microfluídica/métodos , Eletricidade , Hidrodinâmica , Microesferas , Tamanho da Partícula , Fatores de Tempo
5.
J Chromatogr A ; 1624: 461256, 2020 Aug 02.
Artigo em Inglês | MEDLINE | ID: mdl-32540082

RESUMO

Novel Psychoactive Substances (NPS) represent an alternative to established illicit drugs. They are traded via the internet and exhibit small alterations in their chemical structure to circumvent law, however, their psychotropic effects are comparable. There is still poor knowledge about side effects and health risks. By the end of 2018, 730 NPS were reported to EMCDDA (European Monitoring Centre for Drugs and Drug Addiction). Among different compound classes, many NPS are chiral and few publications deal with the different pharmacological and toxicological properties of their pure enantiomers. Therefore, analytical method development concerning enantioseparation of NPS is of great interest. Chiral separation protocols of established illicit drugs have been transferred for NPS, selected examples are given as well. Different methods for enantioseparation of NPS comprising mainly stimulating drugs such as cathinones, pyrovalerones, amphetamines, ketamines, (2-aminopropyl) benzofuranes, phenidines, phenidates, morpholines and thiophenes are reviewed. Moreover, chiral resolution of some cannabinomimetics by HPLC is presented. Chromatographic and electrophoretic techniques such as GC, HPLC, SFC, CE and CEC are discussed and in some cases compared. Mainly, solid samples either purchased from internet vendors, seized by police or collected from patients in hospitals are subject to analysis. Chiral selectors used for HPLC are listed in a Table. It was shown that particularly stimulating drugs are traded as racemic mixtures, which is not the case with cannabinomimetics. Mainly, HPLC and CE were used for enantioseparation of NPS.


Assuntos
Cromatografia/métodos , Eletroforese/métodos , Psicotrópicos/química , Psicotrópicos/isolamento & purificação , Humanos , Estereoisomerismo
6.
Artigo em Russo | MEDLINE | ID: mdl-32592567

RESUMO

AIM OF STUDY: To study of the effect of the combined impact of nicotinic acid electrophoresis and air bubble baths on the state of microcirculation (MC) in patients with diabetic polyneuropathy (DPN). MATERIALS AND METHODS: 60 patients with DPN and type 2 diabetes mellitus with a disease duration of 6 to 25 years were examined and treated. All patients were divided into 2 groups: 30 patients of the 1st group received the combined impact of nicotinic acid electrophoresis and air-bubble baths; 30 patients of the 2nd group - only electrophoresis of nicotinic acid according to Vermel. The dynamics of the MC state was evaluated according to laser Doppler flowmetry (LDF). RESULTS: Before treatment, in most patients with DPN (98%), according to LDF, a spastic type of MC was established. After a course of treatment, it was found that the combined use of nicotinic acid electrophoresis and air bubble baths in patients with DPN has a more pronounced corrective effect on the condition of the MC than the use of Vermel's electrophoresis of nicotinic acid only. CONCLUSION: Complex therapy, including electrophoresis of nicotinic acid and air bubble baths, has a pronounced effect on the condition of the MC in patients with DPN and is pathogenetically justified for the treatment of this category of patients.


Assuntos
Balneologia , Diabetes Mellitus Tipo 2 , Neuropatias Diabéticas , Eletroforese , Niacina , Banhos , Neuropatias Diabéticas/terapia , Humanos , Microcirculação
7.
Electrophoresis ; 41(13-14): 1137-1151, 2020 07.
Artigo em Inglês | MEDLINE | ID: mdl-32469436

RESUMO

The material properties of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its proteins are discussed. We review the viral structure, size, rigidity, lipophilicity, isoelectric point, buoyant density and centrifugation conditions, stability against pH, temperature, UV light, gamma radiation, and susceptibility to various chemical agents including solvents and detergents. Possible inactivation, downstream, and formulation conditions are given including suitable buffers and some first ideas for quality-control methods. This information supports vaccine development and discussion with competent authorities during vaccine approval and is certainly related to drug-targeting strategies and hygienics. Several instructive tables are given, including the pI and grand average of hydropathicity (GRAVY) of SARS-CoV-1 and -2 proteins in comparison. SARS-CoV-1 and SARS-CoV-2 are similar in many regards, so information can often be derived. Both are unusually stable, but sensitive at their lipophilic membranes. However, since seemingly small differences can have strong effects, for example, on immunologically relevant epitope settings, unevaluated knowledge transfer from SARS-CoV-1 to SARS-CoV-2 cannot be advised. Published knowledge regarding downstream processes, formulations and quality assuring methods is, as yet, limited. However, standard approaches employed for other viruses and vaccines seem to be feasible including virus inactivation, centrifugation conditions, and the use of adjuvants.


Assuntos
Betacoronavirus/química , Proteínas Virais/química , Vacinas Virais/farmacologia , Animais , Betacoronavirus/efeitos dos fármacos , Betacoronavirus/efeitos da radiação , Desinfetantes/farmacologia , Eletroforese , Temperatura Alta , Humanos , Concentração de Íons de Hidrogênio , Ponto Isoelétrico , Raios Ultravioleta , Vacinas Atenuadas/imunologia , Vacinas Atenuadas/farmacologia , Vacinas Virais/imunologia , Inativação de Vírus/efeitos da radiação
8.
Ecotoxicol Environ Saf ; 197: 110620, 2020 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-32311615

RESUMO

The aim of this study was to evaluate the ecotoxic effect of high concentration cesium (Cs) exposure on plant root growth and its toxicological mechanism. The radicle of broad bean (Vicia faba) was selected as experimental material. The cytotoxic and genotoxic effects of plants exposed to different Cs levels (0.19-1.5 mM) for 48 h were evaluated using scanning electron microscopy (SEM), X-ray fluorescence (XRF) analysis, single-cell gel electrophoresis (SCGE) and random amplified polymorphic DNA (RAPD) assays. The results showed that radicle elongation decreased clearly after 48 h of exposure treatment with different concentrations of Cs solution. The root cell structure was obviously damaged in the Cs treatment groups (0.19-1.5 mM). At a Cs concentration of 1.5 mM, the percentages of viable non-apoptotic cells, viable apoptotic cells, non-viable apoptotic cells, and non-viable cells were 40.09%, 20.67%, 28.73%, and 10.52%, respectively. SCGE showed DNA damage in radicle cells 48 h after Cs exposure. Compared with the control group, the percentage of tail DNA in Cs exposed group (0.38-1.5 mM) increased by 0.56-1.12 times (P < 0.05). RAPD results showed that the genomic stability of V. faba radicles decreased by 4.44%-15.56%. This study confirmed that high concentration Cs exposure had cytotoxicity and genotoxicity effects on plants.


Assuntos
Césio/toxicidade , Dano ao DNA , Vicia faba/efeitos dos fármacos , Apoptose , Citotoxinas/toxicidade , Eletroforese , Instabilidade Genômica/efeitos dos fármacos , Raízes de Plantas/efeitos dos fármacos , Técnica de Amplificação ao Acaso de DNA Polimórfico , Análise de Célula Única , Vicia faba/genética
9.
Food Chem ; 321: 126677, 2020 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-32247180

RESUMO

Myosin heavy chain (MHC) isoforms in goat muscles and their possible relationships with meat quality have not been fully elucidated. This study characterized the MHC isoforms in different caprine muscles using sodium dodecyl sulphate glycerol gel electrophoresis (SDS-GGE). The relationships between MHC isoforms, calpain systems and meat quality characteristics of different muscles in goats were examined. Four muscles, namely infraspinatus (IF), longissimus dorsi (LD), psoas major (PM) and supraspinatus (SS) were obtained from ten Boer crossbred bucks (7-10 months old; 26.5 ± 3.5 kg, BW). The percentages of MHC I, MHC IIa and MHC IIx in SS, IF, PM and LD were 47.2, 38.3, 32.1, 11.9; 28.0, 42.1, 33.0, 36.4; and 24.8, 19.6, 34.9 and 51.7, respectively. IF and SS had higher levels of calpastatin, total collagen and insoluble collagen contents than did PM and LD. PM had longer sarcomere length than did other muscles. LD had higher collagen solubility, troponin-T degradation products and glycogen content than did other muscles. These results infer that variable fiber-type composition could account partially for the differences in the physicochemical properties of goat muscles.


Assuntos
Calpaína/metabolismo , Músculo Esquelético/metabolismo , Cadeias Pesadas de Miosina/metabolismo , Animais , Proteínas de Ligação ao Cálcio/metabolismo , Eletroforese , Eletroforese em Gel de Poliacrilamida , Cabras , Carne/análise , Músculo Esquelético/química , Isoformas de Proteínas/metabolismo , Troponina T/metabolismo
10.
Med. clín (Ed. impr.) ; 154(7): 275-278, abr. 2020. graf
Artigo em Espanhol | IBECS | ID: ibc-190912

RESUMO

INTRODUCCIÓN Y OBJETIVOS: La analbuminemia congénita (AAC) (MIM #616000) es una enfermedad autosómica recesiva (prevalencia <1/106) causada por defectos en el gen ALB que implican la ausencia o marcada disminución de la albuminemia. En este artículo, describimos un caso de AAC detectado en nuestro hospital. MATERIAL Y MÉTODOS: Mujer de 42 años con hipoproteinemia e hipoalbuminemia de causa no filiada. El estudio bioquímico se realizó siguiendo las técnicas y los controles de calidad habituales de nuestro laboratorio: albuminemia (colorimetría y nefelometría); electroforesis de proteínas (capilar y gel de agarosa) y análisis molecular del gen ALB (extracción de ADN y amplificación PCR de los 14 exones codificantes más regiones intrónicas adyacentes y secuenciación Sanger). RESULTADOS: Descartadas las causas más frecuentes de hipoalbuminemia, se confirmó la analbuminemia por electroforesis y nefelometría. El estudio molecular del gen ALB evidenció la presencia de la variante c.1289+1G>A (variante Guimarães) en homozigosis. CONCLUSIONES: Este es el primer caso confirmado mediante estudio molecular de AAC en España. La paciente presenta la variante Guimarães descrita previamente en otros 4 pacientes en el mundo


INTRODUCTION AND OBJECTIVES: Congenital analbuminaemia (CCA) (MIM #616000) is an autosomal recessive disorder (prevalence < 1/106) caused by defects in the ALB gene leading to absence or severe reduction of albuminaemia. This paper describes a case of CCA detected and diagnosed in our hospital. MATERIALS AND METHODS: A 42-year old woman showing hypoproteinaemia and hypoalbuminaemia of unknown aetiology. Biochemical study was performed according to routine quality controlled analytical procedures: Albuminaemia (colorimetric and nephelometric methods). Protein electrophoresis (capillary and agarose gel). Molecular study of the ALB gene: DNA extraction, PCR amplification of the 14 coding exons plus adjacent intron regions and Sanger sequencing. RESULTS: After discarding the most common causes of hypoalbuminaemia, the analbuminaemia was confirmed by nephelometry and protein electrophoresis. The proband was found to be homozygous for molecular defect in the ALB gene: variant c.1289+1G>A previously reported as Guimarães variant. CONCLUSIONS: This is the first case of CCA confirmed by molecular study in Spain. The proband shows the Guimarães variant previously described in 4 patients worldwide


Assuntos
Humanos , Feminino , Adulto , Doenças Genéticas Inatas/diagnóstico , Técnicas de Diagnóstico Molecular/métodos , Albumina Sérica/genética , Doenças Genéticas Inatas/genética , Eletroforese , Hipoalbuminemia/etiologia , Colorimetria/métodos , Nefelometria e Turbidimetria/métodos , Diagnóstico Diferencial
11.
Ann Biol Clin (Paris) ; 78(1): 17-26, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-32108576

RESUMO

The lipoproteinogram (or lipidogram) consists in an electrophoretic separation of the main classes of serum lipoproteins. Separation was done in agarose gel using the Sebia Hydragel Lipo + Lp(a)® kit. A repeatability study (n=6) was conducted on 3 sera (1 normolipidemic, 1 hypertriglyceridemic and 1 with a high Lp(a) concentration). The reproducibility was studied on these 3 sera and on an ascites liquid containing chylomicrons, upon 6 days (n=6). A quantitative approach was made by studying areas under the curve and percentages of fractions. In both cases (repeatability and reproducibility), the revelation of the lipoproteins in the gel after electrophoretic migration was made either by staining with Sudan Black (procedure recommended by Sebia), or with Fat Red 7B. Regardless of staining, both repeatability and reproducibility studies show that all lipoprotein fractions were correctly detected at their respective positions, leading to satisfactory interpretations of lipoproteinograms. Our reproducibility study also confirmed a good stability of the fractions over 6 days (storage at +5 ± 3̊C). In addition, the Fat Red 7B staining leads to a shorter technical time (about 40 min) for the gel drying and staining/destaining phases, which allows us to respond more quickly to certain urgent requests such as chylothorax diagnosis.


Assuntos
Compostos Azo/farmacologia , Eletroforese/métodos , Lipoproteínas/análise , Lipoproteínas/sangue , Kit de Reagentes para Diagnóstico , Coloração e Rotulagem/métodos , Líquido Ascítico/química , Compostos Azo/química , Análise Química do Sangue/métodos , Fracionamento Químico/métodos , Eletroforese em Gel de Ágar , Humanos , Lipoproteína(a)/análise , Lipoproteína(a)/sangue , Reprodutibilidade dos Testes
12.
Ann Biol Clin (Paris) ; 78(1): 87-92, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-32108586

RESUMO

Serum proteins and urinary proteins electrophoresis are useful biological tests. They are often prescribed for the screening of monoclonal gammopathys and also during follow-up for treatment response. This test can be accredited according to standard NF ISO 15189 since laboratories use analysers and adapted reagents, but there are numerous protocols for the method validation. This paper present the results of a survey proposed in 2019 to biologists by CNBH and SFBC. The aim of this survey is to give biologists a choice among several protocols that have been positively evaluated by COFRAC.


Assuntos
Acreditação , Análise Química do Sangue/normas , Eletroforese/normas , Laboratórios/normas , Urinálise/normas , Análise Química do Sangue/métodos , Proteínas Sanguíneas/análise , Proteínas Sanguíneas/química , Comportamento de Escolha , Testes Diagnósticos de Rotina/normas , Eletroforese/métodos , Humanos , Ensaio de Proficiência Laboratorial/métodos , Proteinúria/diagnóstico , Proteinúria/urina , Controle de Qualidade , Reprodutibilidade dos Testes , Estudos Retrospectivos , Sensibilidade e Especificidade , Inquéritos e Questionários , Urinálise/métodos , Estudos de Validação como Assunto
13.
J Chromatogr A ; 1620: 460972, 2020 Jun 07.
Artigo em Inglês | MEDLINE | ID: mdl-32106967

RESUMO

This paper reports for the first time the coupling of a two-dimensional separation technique known as orthogonal pressurised planar electrochromatography (OPPEC) with on-line UV/VIS detection. It establishes the efficiency characteristic of a planar column used in OPPEC. The studies show that in OPPEC, all separation steps (mixture introduction, separation, and detection) can run successfully as non-separate operation, much like in HPLC, and the planar column has itself good efficiency, ensuring a relatively low plate height value. In its use, some findings of the previously proposed theoretical model concerning optimisation of preparative separation of components with the same electrophoretic mobility are verified - showing that there is a good agreement between theory and experiment. The paper also demonstrates by means of a specific example of separation of a post-reaction mixture obtained after synthesis of valine diastereomers that OPPEC can be a powerful tool used in preparative separation in that it can achieve much higher separation productivity and significantly lower eluent consumption than when using column chromatography techniques.


Assuntos
Cromatografia/métodos , Eletroforese/métodos , Estereoisomerismo
14.
Talanta ; 211: 120673, 2020 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-32070558

RESUMO

In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals. The effects of some typical parameters such as sample volume, applied voltage, composition and pH of the running buffer and organic modifier concentration were examined and optimized. Moreover, the impact of type and concentration of cyclodextrin as electrolyte modifiers was also investigated. The detection limits of analytes for the elaborated LVSS-PS-CDEKC method were found to be in 0.8-5 ng mL-1 range, which were around 500 times lower than normal CDEKC without preconcentration technique. All analytes were completely resolved in less than 11 min in an uncoated fused-silica capillary of 75 µm internal diameter (I.D) x 50 cm length. The electrophoretic separation was performed in a 2 mM α-cyclodextrin and 25 mM tetraborate system (pH = 9.3) with an applied voltage of 25 kV. The established method was validated and confirmed to be applicable for the determination of the preservatives in a quality control of pharmaceuticals.


Assuntos
Ácido Benzoico/análise , Hidroxibenzoatos/análise , Parabenos/análise , Conservantes Farmacêuticos/análise , Ácido Sórbico/análise , Ambroxol/análise , Boratos , Cromatografia Capilar Eletrocinética Micelar , Eletroforese , Cetotifeno/análise , Fenilbutiratos/análise , alfa-Ciclodextrinas
15.
Vet Immunol Immunopathol ; 221: 110014, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32004910

RESUMO

Immunofixation is a diagnostic and research tool used for characterizing the electrophoretic location of immunoglobulin fractions in serum and urine. Commercially available polyclonal antisera which discriminate two IgG subclasses (IgG1 and IgG2) are available and commonly used. More recently, four IgG subclasses have been defined in the dog based on cDNA data. Archived serum from 16 dogs with naturally occurring monoclonal or biclonal gammopathies were characterized using routine serum protein electrophoresis, routine immunofixation and LCMS/MS as 3 IgA, 3 IgM, 2 IgG2, 7 IgG3 and 2 IgG4 heavy chain predominant cases. Immunofixation reactivity of a panel of commercially available antisera to these cases was characterized. The anti-human IgG antisera was the only tested antisera which bound all canine IgG restricted bands without also labelling IgA or IgM heavy chains or light chains. The tested polyclonal antisera labeled as reacting with canine IgG2 bound canine IgG2, IgG3, IgA and IgM and may label IgG1. The tested polyclonal antisera labeled as reacting with canine IgG1 bound the canine IgG4 bands but not those identified as IgA, IgM, IgG2 or IgG3 and likely did not bind IgG1. This data suggests that commercially available polyclonal IgG1 antisera (Bethyl A40 - 120A and Bio-Rad AHP947) can be used to positively but possibly not selectively identify canine IgG4 by immunofixation.


Assuntos
Soros Imunes/análise , Imunoglobulina G/sangue , Imunoglobulina G/classificação , Animais , Cromatografia Líquida , Cães , Eletroforese , Humanos , Testes Imunológicos , Masculino
16.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 28(1): 191-195, 2020 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-32027275

RESUMO

OBJECTIVE: To analyzed the prognostic value of serum free light chain kappa/lambda ratio detection combined with immunofixation electrophoresis in multiple myeloma (MM) patients. METHODS: 72 patients with MM treated in our hospital from January 2017 to December 2018 were selected. Serum free light chain kappa/lambda ratio (sFLCR) and immune typing were detected respectively. The clinical characteristics and survival time were compared among patients. COX regression was used to analyze the factors influencing prognosis. RESULTS: 38 patients showed high sFLCR, and 34 showed low sFLCR. Compared with the low sFLCR group, the DS stage of patients in high sFLCR group elevated, the levels of ß2-MG and Scrwere increased, and Hb decreased, all the differences were statistically significant (P<0.05). Among 72 patients, there were 40 cases of IgG type (55.56%), 27 cases of IgA type (37.50%) and 5 cases of IgM type (6.94%). Compared with IgG and IgA patients, the serum calcium and creatinine in IgM patients were increased significantly, while Hb decreased significantly (P<0.05). The median survival time was 19.2 months in 21 patients with IgG type and high sFLCR; 24.0 months in 19 patients with IgG type and low sFLCR; 15.0 months in 12 patients with IgA type and high sFLCR; 16.7 months in 15 patients with IgA type and low sFLCR; 6.0 months in 5 patients with IgM type and high sFLCR,respectively. DS stage, M protein typing and sFLCR correlated with prognosis of patients (P<0.05). CONCLUSION: The serum free light chain kappa/lambda ratio combined with immunofixation electrophoresis is valuable for the prognostic evaluation of patients with multiple myeloma.


Assuntos
Mieloma Múltiplo , Eletroforese , Humanos , Cadeias Leves de Imunoglobulina , Cadeias kappa de Imunoglobulina , Cadeias lambda de Imunoglobulina , Prognóstico
17.
Int J Artif Organs ; 43(2): 127-136, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32000591

RESUMO

INTRODUCTION: The aim of this study was to develop a novel decellularization method in order to obtain an ideal scaffold with good biocompatibility. METHODS: The porcine corneas were treated with human serum for 5 days or serum-electrophoresis respectively. The electrophoresis (100 V/cm) was performed in sterilized buffer containing 40-mM tris base, 18-mM glacial acetic acid, and antibiotics for 1 h at 4°C. The properties of artificial corneal scaffolds were characterized by morphological and histological examinations. The biocompatibility and biological safety were examined by subcutaneous implant test and lamellar keratoplasty. RESULTS AND CONCLUSIONS: The transparency and appearance of serum-electrophoresis acellular porcine corneal matrix were better than serum acellular porcine corneal matrix. DNA and α-gal in serum-electrophoresis acellular porcine corneal matrix were more efficiently removed than those in serum acellular porcine corneal matrix (p < 0.05). The subcutaneous and corneal implantation experiments showed serum-electrophoresis acellular porcine corneal matrix had better biocompatibility compared to serum acellular porcine corneal matrix (p < 0.01). This novel serum-electrophoresis decellularization method may be valuable for preparation of xenogenic corneal tissue for clinical application.


Assuntos
Materiais Biocompatíveis , Córnea , Transplante de Córnea/métodos , Engenharia Tecidual/métodos , Tecidos Suporte , Animais , Córnea/citologia , Córnea/cirurgia , Eletroforese/métodos , Humanos , Teste de Materiais/métodos , Suínos
18.
Allergol. immunopatol ; 48(1): 26-33, ene.-feb. 2020. ilus, graf
Artigo em Inglês | IBECS | ID: ibc-186588

RESUMO

Introduction and Objectives: The production and consumption of oysters is increasing annually because it can provide essential nutrients and benefit for human health, leading to frequent occurrence of severe allergic reactions observed in sensitized individuals. The aim of the present study was to investigate the effects of acid and protease treatment on the conformation and IgE-binding capacity of recombinant Crassostrea gigas tropomyosin (Cra g 1). Results: Under acidic conditions, Cra g 1 did not undergo degradation, however, the changes obvious in the intensity of CD signal and ANS-binding fluorescence were observed, which was associated with a decrease in antibody reactivity. In simulated gastrointestinal fluid (SGF) and simulated intestinal fluid (SIF) digestion system, acid-treated Cra g 1 was relatively resistant to digestion, but the degradative patterns were very different. Moreover, owing to alterations of secondary structure and hydrophobic surface of the protein during digestive processing, antigenicity of acid-induced Cra g 1 reduced in SGF while it increased significantly in SIF. Conclusion: To our knowledge, this is the first study reporting that antigenicity of acid-treated oyster tropomyosin increased after SIF digestion. These results revealed that treatment with acid and pepsin, rather than trypsin, was an effective way of reducing IgE-binding capacity of tropomyosin from oyster


No disponible


Assuntos
Humanos , Imunoglobulina E/imunologia , Técnicas In Vitro/métodos , Alérgenos/química , Tropomiosina/química , Hipersensibilidade Alimentar/imunologia , Digestão , Alérgenos/imunologia , Tropomiosina/imunologia , Ostreidae/imunologia , Sistema Digestório/imunologia , Eletroforese/métodos , Análise Espectral/métodos , Ensaio de Imunoadsorção Enzimática
19.
Colloids Surf B Biointerfaces ; 188: 110763, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31896518

RESUMO

For the first time, an electrophoretic deposition (EPD) method has been developed for the deposition of polymethylmethacrylate (PMMA) and PMMA-alumina films for biomedical implant applications. The proposed biomimetic approach was based on the use of a bile salt, sodium cholate (NaCh), which served as a multifunctional solubilizing, charging, dispersing and film-forming agent. Investigations revealed PMMA-Ch- and PMMA-alumina interactions, which facilitated the deposition of PMMA and PMMA-alumina films. This approach allows for the use of a non-toxic water-ethanol solvent for PMMA. The proposed deposition strategy can also be used for co-deposition of PMMA with other functional materials. The PMMA and composite films were tested for biomedical implant applications. The PMMA-alumina films showed statistically improved metabolic results compared to both the bare stainless steel substrate and pure PMMA films. Alkaline phosphatase (ALP) activity affirmed the bioactivity and osteoconductive potential of PMMA and composite films. PMMA-alumina films showed greater ALP activity than both the PMMA-coated and uncoated stainless steel.


Assuntos
Pesquisa Biomédica , Osteossarcoma/metabolismo , Polimetil Metacrilato/metabolismo , Fosfatase Alcalina/metabolismo , Óxido de Alumínio/química , Óxido de Alumínio/metabolismo , Eletroforese , Humanos , Osteossarcoma/patologia , Tamanho da Partícula , Polimetil Metacrilato/química , Propriedades de Superfície
20.
Pharm Res ; 37(2): 22, 2020 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-31897766

RESUMO

PURPOSE: The work aimed to compare quality of a siRNA carrier prepared with chitosan of two different sources having similar degree of deacetylation and molecular weights. Differences were analyzed from thermodynamic characteristics of interactions with siRNA. METHODS: The siRNA carrier (chitosan-coated poly(isobutylcyanoacrylate) nanoparticles) was prepared with home-prepared, CSLab, and commercial, CSCom, chitosans. Chitosan counterion was identified and chitosans CSCommod1 and CSCommod2 were obtained from CSCom exchanging counterion with that found on CSLab. Carrier quality was checked considering the size, zeta potential and siRNA association capacity by gel electrophoresis. Thermodynamic parameters of interactions between siRNA and chitosans in solution or immobilized at the carrier surface were determined by isothermal titration calorimetry (ITC). RESULTS: CSLab and CSCommod2 having a high content of acetate counterion associated better siRNA than CSCom and CSCommod1 which counterion included mainly chloride. ITC measurements indicated that siRNA interactions with chitosan and the siRNA carrier were driven by entropic phenomena including dehydration, but thermodynamic parameters of interactions clearly differed according to the nature of the counterion of chitosan. The influence of chitosan counterions was interpreted considering their different lyotropic character. CONCLUSION: Association of siRNA with our siRNA carrier was influenced by the nature of counterions associated with chitosan. Driven by entropic phenomena including dehydration, interactions were favored by acetate counterion. Although more work would be needed to decipher the influence of the counterion of chitosan during association with siRNA, it was pointed out as a new critical attribute of chitosan to consider while formulating siRNA carrier with this polysaccharide.


Assuntos
Quitosana/química , RNA Interferente Pequeno/química , Calorimetria/métodos , Eletroforese/métodos , Nanopartículas/química , Termodinâmica
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