Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 333
Filtrar
Mais filtros










Intervalo de ano de publicação
1.
Food Chem ; 306: 125615, 2020 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-31622833

RESUMO

Phycocyanin (PC), a plant-based protein with interesting biological activity, is rarely directly applied in the food industry because it has structural and functional limitations. This study combined ultra-high-pressure (UHP) treatment with glycation to improve PC functionality and explored resulting structural changes using sodium dodecyl sulfate-polyacrylamide gel electrophoresis, scanning electron microscopy, Fourier-transform infrared spectroscopy, circular dichroism, and UV-visible spectroscopy. The UHP treatment obviously improved the speed and degree of glycation and the composite-modified PC (CM-PC) showed high solubility and good emulsifying and foaming performance. Scanning electron microscopy images showed the CM-PC surface was loose and fluffy. Gel electrophoresis, Fourier-transform infrared spectroscopy, and circular dichroism results demonstrated that the content of α-helix decreased from 78.1% in PC to 26.6% in CM-PC, and hydroxyl groups were introduced. UV-visible spectroscopy showed that the mechanism of composite modification involved stretching of the PC and promotion of binding with sugars.


Assuntos
Ficocianina/química , Spirulina/química , Dicroísmo Circular , Eletroforese em Gel de Poliacrilamida , Emulsões/metabolismo , Glicosilação , Pressão , Solubilidade , Espectroscopia de Infravermelho com Transformada de Fourier
2.
J Sci Food Agric ; 100(1): 129-138, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31452202

RESUMO

BACKGROUND: Capsaicin, as a major pungent ingredient of peppers, has many health benefits. However, the strong irritation effect of capsaicin inhibits its application in the food industry. Emulsions can be an effective approach to alleviate the irritation. RESULTS: In this study, we used tocopheryl polyethylene glycol 1000 succinate (TPGS) as an emulsifier to prepare capsaicin emulsions through high-pressure homogenization. Capsaicin emulsions with a particle size of about 100 nm, -36.4 mV zeta potential, and 91.9% encapsulation efficiency were prepared successfully and showed better environmental stability and higher antioxidant activity. Emulsions reduced the cumulative release of capsaicin and had no toxic effect on buffalo rat liver (BRL-3A) cells. Moreover, the gastrointestinal injury model of rats showed that emulsions reduced the strong irritation of capsaicin. CONCLUSION: This work provides a theoretical basis for the application of irritant ingredients in food industry. © 2019 Society of Chemical Industry.


Assuntos
Capsaicina/química , Capsaicina/metabolismo , Trato Gastrointestinal/metabolismo , Animais , Emulsificantes/química , Emulsões/química , Emulsões/metabolismo , Masculino , Tamanho da Partícula , Ratos , Ratos Sprague-Dawley , Vitamina E/química
3.
J Agric Food Chem ; 67(34): 9543-9550, 2019 Aug 28.
Artigo em Inglês | MEDLINE | ID: mdl-31379164

RESUMO

This study aimed to reveal the mechanisms underlying the interaction between condensed tannins (CTs) and bile salts. The interaction mechanism was analyzed by transmission electron microscopy, exposure to various physicochemical conditions, electrophoresis, fluorescence spectroscopy, isothermal titration calorimetry, and molecular modeling. A new complex was formed from CTs and bile salts. The complex showed a negative enthalpy change and a positive entropy change, demonstrating that the main thermodynamic driving force was both entropy and enthalpy and indicating that binding occurred through hydrogen bonds and hydrophobic interactions. The analysis of the effects of CTs on the stability and digestion properties of bile salt emulsions indicated that CTs were able to inhibit lipid digestion to an extent. Our findings may provide evidence that foods rich in CTs offer health benefits by aggregating with bile salts and reducing the absorption of fat.


Assuntos
Ácidos e Sais Biliares/química , Proantocianidinas/química , Ácidos e Sais Biliares/metabolismo , Calorimetria , Emulsões/química , Emulsões/metabolismo , Entropia , Ligações de Hidrogênio , Interações Hidrofóbicas e Hidrofílicas , Proantocianidinas/metabolismo , Termodinâmica
4.
Int J Food Microbiol ; 306: 108261, 2019 Oct 02.
Artigo em Inglês | MEDLINE | ID: mdl-31302487

RESUMO

Turbidity in beverages is typically achieved by addition of emulsion based cloud systems. Their intrinsic instability necessitates the widespread use of technological measures and use of food additives to prevent emulsion decay. In this work, we explored the possibility to establish a new generation of natural, stable clouding systems based on bacterial dextrans. Lactobacillus hordei TMW 1.1907 originating from water kefir was used to produce dextrans in sucrose supplemented apple or grape juices. By varying the fermentation conditions, two distinct types of dextran molecules could be produced at yields ranging from 2.5 to 8.5 g/L. The dextran-containing fermentates showed an unchanged turbidity after pasteurization at acidic pH and subsequent storage for three months. No sedimentation of particles occurred upon storage. Neutralization of the acidic fruit juices to pH 7 prior to fermentation significantly increased the dextran yields. The molecular weight, rms radii and turbidity of dextrans produced at 20 °C were higher than those produced at 30 °C. Characterization of the isolated dextrans by asymmetric flow field-flow fractionation coupled to multi-angle laser light scattering revealed a random-coil like structure and rms radii ranging from 66.0 to 87.4 nm. The averaged molar masses of the cloud forming dextrans were in the approximate range of 103.1 to 141.6 MDa. In conclusion, our results demonstrate the possibility to ferment fruit juices for in situ production of dextrans exhibiting novel techno-functional properties beyond gelling and thickening.


Assuntos
Dextranos/metabolismo , Fermentação/fisiologia , Aditivos Alimentares/metabolismo , Sucos de Frutas e Vegetais/análise , Frutas/metabolismo , Ácidos/metabolismo , Emulsões/metabolismo , Kefir/microbiologia , Lactobacillus/metabolismo , Malus/metabolismo , Vitis/metabolismo
5.
Carbohydr Polym ; 221: 10-20, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31227148

RESUMO

ß-carotene has been often used as a hydrophobic nutrient in many functional foods owning to its excellent antioxidant activity. However, the poor orally bioavailability of ß-carotene limits its utilization. To overcome such limitation, a delivery system was designed for the encapsulation of ß-carotene based on oil-in-water emulsion stabilized by oat protein isolate - Pleurotus ostreatus ß-glucan Maillard conjugate. The results showed that such conjugate protected emulsion against environmental stresses by increasing steric and electrostatic repulsion between droplets, mainly manifesting as their smaller particle size and higher surface charge. Additionally, conjugate promoted lipid digestion and formation of mixed micelles, leading to an improved gastrointestinal fate of encapsulated ß-carotene, especially for its in vitro bioavailability. Such effects could enhance the cellular antioxidant activity of encapsulated ß-carotene in Caco-2 cells. Our findings confirmed that Maillard conjugate can structure an emulsion-based delivery system for the encapsulation of hydrophobic ingredients to improve their utilization.


Assuntos
Antioxidantes/farmacologia , Emulsões/química , Trato Gastrointestinal/metabolismo , Glucanos/química , Proteínas de Plantas/química , beta Caroteno/farmacologia , Antioxidantes/química , Antioxidantes/metabolismo , Avena/química , Células CACO-2 , Óleo de Milho/química , Óleo de Milho/metabolismo , Portadores de Fármacos/química , Portadores de Fármacos/metabolismo , Emulsões/metabolismo , Glucanos/metabolismo , Temperatura Alta , Humanos , Concentração de Íons de Hidrogênio , Reação de Maillard , Concentração Osmolar , Proteínas de Plantas/metabolismo , Pleurotus/química , beta Caroteno/química , beta Caroteno/metabolismo
6.
J Agric Food Chem ; 67(21): 6032-6040, 2019 May 29.
Artigo em Inglês | MEDLINE | ID: mdl-31083996

RESUMO

In the typical Western diet, fruits and vegetables are often consumed with food products that exist as oil-in-water emulsions, such as creams, dressings, and sauces. Studies have shown that coingestion of fruits and vegetables with emulsions can increase the bioavailability of beneficial lipophilic bioactive agents, such as nutraceuticals or vitamins. Agricultural produce, however, may also be contaminated with low levels of detrimental lipophilic agents, such as hydrophobic pesticides. We therefore examined the impact of coingesting a common agricultural product (tomatoes) with model food emulsions on the bioaccessibility of a hydrophobic pesticide (chlorpyrifos). The impact of emulsifier types (phospholipids, whey protein, Tween 80) and dietary fiber types (xanthan, chitosan, ß-glucan) on the bioaccessibility of the pesticide was measured using a simulated gastrointestinal model. Chlorpyrifos bioaccessibility depended on the type of emulsifier used to formulate the emulsions: phospholipids > Tween 80 > whey protein. Dietary fiber type also influenced pesticide bioaccessibility by an amount that depended on the nature of the emulsifier used. Overall, our results suggest that the bioaccessibility of undesirable pesticides on fruits and vegetables will depend on the nature of the emulsions they are consumed with.


Assuntos
Produtos Biológicos/metabolismo , Fibras na Dieta/metabolismo , Emulsificantes/química , Lycopersicon esculentum/metabolismo , Resíduos de Praguicidas/metabolismo , Disponibilidade Biológica , Produtos Biológicos/química , Óleo de Milho/química , Óleo de Milho/metabolismo , Fibras na Dieta/análise , Digestão , Emulsificantes/metabolismo , Emulsões/química , Emulsões/metabolismo , Contaminação de Alimentos/análise , Trato Gastrointestinal/metabolismo , Humanos , Lycopersicon esculentum/química , Modelos Biológicos , Tamanho da Partícula , Resíduos de Praguicidas/química
7.
Pharm Dev Technol ; 24(8): 982-991, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31107131

RESUMO

Tanshinone I (TSI) is one of the bioactive compound obtained from the root of Salvia miltiorrhiza which is a well-known traditional Chinese medicine (TCM) used for the treatment of various diseases. Although TSI possesses several pharmacological effects, it has poor water solubility, blood-brain barrier (BBB) permeability and brain bioavailability. Therefore, in the present study, we developed TSI nanoemulsion (TSI-NE) modified with a brain targeting ligand (Lactoferrin (Lf)) to improve the BBB permeability. Pseudo-ternary phase diagrams were used to optimize the formulation. The optimal TSI-NE and TSI-Lf-NE were prepared and characterized. Finally, the uptake of TSI-Lf-NE by mouse brain microvascular endothelial cell line (bEnd.3 cells) was assessed using Coumarin-6 as a fluorescent probe. The results of the study showed that the stable optimal formulation of O/W nanoemulsion was successfully developed and modified with Lf. The cellular uptake study has shown that the fluorescence intensity (FI) increased with time over the incubation period. The FI at all time intervals increased in the following order: Coumarin-6-Solution<Coumarin-6-NE<Coumarin-6-Lf-NE. The results suggest that the BBB permeability of Coumarin-6-Lf-NE was better than those of Coumarin-6-NE and Coumarin-6 solution. Lf modified nanoemulsion has great potential for improving the brain delivery of TSI.


Assuntos
/química , Encéfalo/metabolismo , Emulsões/química , Lactoferrina/química , Nanopartículas/química , Animais , Disponibilidade Biológica , Transporte Biológico/efeitos dos fármacos , Barreira Hematoencefálica/metabolismo , Linhagem Celular , Cumarínicos/química , Cumarínicos/metabolismo , Portadores de Fármacos/química , Sistemas de Liberação de Medicamentos/métodos , Emulsões/metabolismo , Lactoferrina/metabolismo , Camundongos , Nanopartículas/metabolismo , Tamanho da Partícula , Permeabilidade/efeitos dos fármacos , Polietilenoglicóis/química , Solubilidade , Tiazóis/química , Tiazóis/metabolismo , Distribuição Tecidual/efeitos dos fármacos
8.
Food Funct ; 10(6): 3344-3355, 2019 Jun 19.
Artigo em Inglês | MEDLINE | ID: mdl-31095149

RESUMO

The impact of phytic acid on lipid digestion and curcumin bioaccessibility in oil-in-water nanoemulsions was investigated using a simulated gastrointestinal tract (GIT). The size, charge, and structural organization of the colloidal particles in the system were measured as the curcumin-loaded emulsions (7 mg curcumin per g lipid) were passed through simulated mouth (pH 6.8, 2 min), stomach (pH 2.5, 2 hours), and small intestine (pH 7.0, 2 hours) stages. After the small intestine stage, the level of free fatty acids (FFAs) generated and the bioaccessibility of curcumin were measured. The total amount of FFAs released significantly decreased with increasing phytic acid level, from 105.7 ± 5.9% (control) to 78.4 ± 6.4% (0.5% phytic acid). Conversely, curcumin bioaccessibility significantly increased from 39.4 ± 3.5% (control) to 74.7 ± 2.6% (0.5% phytic acid). The inverse relationship between lipolysis and curcumin bioaccessibility was ascribed to the impact of phytic acid on droplet flocculation and the level of free calcium ions present, which affected the production of mixed micelles capable of solubilizing the nutraceutical. The knowledge obtained here might prove beneficial for the employment of phytic acid as a multifunctional ingredient that inhibits lipid digestion while boosting nutraceutical bioavailability.


Assuntos
Curcumina/metabolismo , Trato Gastrointestinal/metabolismo , Gotículas Lipídicas/química , Gotículas Lipídicas/metabolismo , Ácido Fítico/química , Extratos Vegetais/metabolismo , Disponibilidade Biológica , Curcumina/química , Emulsões/química , Emulsões/metabolismo , Excipientes/química , Excipientes/metabolismo , Humanos , Modelos Biológicos , Tamanho da Partícula , Ácido Fítico/metabolismo , Extratos Vegetais/química
9.
J Agric Food Chem ; 67(17): 4931-4939, 2019 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-30931562

RESUMO

Titanium dioxide (TiO2) is used as an additive to whiten some foods and beverages that contain lipid nanoparticles. We therefore investigated the combined influence of TiO2 and lipid nanoparticles on carotenoid bioaccessibility from tomatoes. TiO2 nanoparticles ( d = 167 nm) were combined with nanoemulsions ( d ≈ 150 nm) stabilized by various emulsifiers: Tween 80, whey protein, or sodium caseinate. The mixed systems were then mixed with tomatoes and passed through a simulated gastrointestinal tract (GIT). The emulsifier type significantly influenced carotenoid bioaccessibility ( p < 0.05), mainly because of differences in the ability of the emulsifier-coated lipid droplets to extract carotenoids from tomatoes and form mixed micelles. TiO2 addition did not impact lipid digestion and carotenoid bioaccessibility ( p > 0.05). These results suggested that carotenoid bioaccessibility was not influenced by TiO2 addition but did depend on the type of emulsifier used to stabilize lipid nanoparticles.


Assuntos
Carotenoides/química , Carotenoides/metabolismo , Trato Gastrointestinal/metabolismo , Lycopersicon esculentum/metabolismo , Nanopartículas/química , Titânio/química , Caseínas/química , Digestão , Emulsificantes/química , Emulsões/química , Emulsões/metabolismo , Humanos , Lipídeos/química , Lycopersicon esculentum/química , Tamanho da Partícula , Polissorbatos/química , Proteínas do Soro do Leite/química
10.
J Agric Food Chem ; 67(16): 4524-4534, 2019 Apr 24.
Artigo em Inglês | MEDLINE | ID: mdl-30945544

RESUMO

Dietary polyphenols have shown hypolipidemic effects by reducing triglyceride absorption. The mechanisms may involve modifying fat emulsion during digestion in the gastrointestinal tract and suppressing lipase during hydrolysis in the small intestine. In an in vivo study, lotus seedpod oligomeric procyanidin (LSOPC) decreased total serum triglyceride and total cholesterol and elevated the high-density lipoprotein level in the hyperlipidemic rat model. In addition, LSOPC suppressed de novo lipogenesis-related gene expressions. In an in vitro study, the LSOPC-enriched emulsion decreased the mean droplet size from 0.36 to 0.33 µm and increased the viscosity of the emulsion. Moreover, the LSOPC-enriched emulsion improved the antioxidant properties. A digestion model was developed and showed that the particle size of the LSOPC-enriched emulsion increased in the oral cavity. However, an increase and then a significant drop of the particle size was measured in the stomach and small intestine. The free fatty acid release rate was decreased in the LSOPC-enriched emulsion partly ascribed to the inhibition of lipase by LSOPC.


Assuntos
Biflavonoides/metabolismo , Catequina/metabolismo , Gorduras/metabolismo , Hiperlipidemias/dietoterapia , Metabolismo dos Lipídeos , Lotus/metabolismo , Extratos Vegetais/metabolismo , Proantocianidinas/metabolismo , Animais , Biflavonoides/química , Catequina/química , Digestão , Emulsões/química , Emulsões/metabolismo , Gorduras/química , Mucosa Gástrica/metabolismo , Homeostase , Humanos , Hiperlipidemias/genética , Hiperlipidemias/metabolismo , Hiperlipidemias/fisiopatologia , Mucosa Intestinal/metabolismo , Intestinos , Lotus/química , Masculino , Camundongos , Camundongos Endogâmicos ICR , Tamanho da Partícula , Extratos Vegetais/química , Proantocianidinas/química , Ratos , Ratos Sprague-Dawley , Sementes/química , Sementes/metabolismo
11.
Food Funct ; 10(4): 1826-1835, 2019 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-30874272

RESUMO

The influence of carrier oil type (corn, fish, or flaxseed oil) on the production, stability, and simulated gastrointestinal behavior of vitamin-fortified nanoemulsions was studied. The nanoemulsions were formulated using pea protein as an emulsifier since there is increasing interest in substituting artificial and animal-based food ingredients with natural plant-based alternatives. Lipid digestion and vitamin D3 bioaccessibility were measured when the nanoemulsions were subjected to a three-stage in vitro gastrointestinal tract: oral, gastric, and small intestinal. The majority of all three lipids were digested within the first few minutes in the simulated small intestine, with the corn oil nanoemulsions being digested faster than the fish or flaxseed oils. Moreover, a greater fraction of triglycerides were digested by the end of the small intestine for the corn oil than for the fish and flaxseed oils. For the different carrier oils, vitamin bioaccessibility was ranked: corn oil > flaxseed oil ≈ fish oil. These results suggest that monounsaturated-rich oils (such as corn oil) are better for encapsulating and delivering vitamin D3 than polyunsaturated-rich ones (such as flaxseed or fish oil). The insights gained here may aid in the formulation of more efficacious vitamin-fortified foods and beverages from plant-derived ingredients.


Assuntos
Colecalciferol/química , Colecalciferol/metabolismo , Óleo de Milho/química , Portadores de Fármacos/química , Sistemas de Liberação de Medicamentos/métodos , Óleos de Peixe/química , Óleo de Semente do Linho/química , Proteínas de Plantas/química , Disponibilidade Biológica , Óleo de Milho/metabolismo , Digestão , Portadores de Fármacos/metabolismo , Sistemas de Liberação de Medicamentos/instrumentação , Emulsões/química , Emulsões/metabolismo , Óleos de Peixe/metabolismo , Alimentos Fortificados/análise , Humanos , Óleo de Semente do Linho/metabolismo , Nanoestruturas/química , Ervilhas/química , Proteínas de Plantas/metabolismo
12.
Drug Deliv ; 26(1): 168-178, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30822166

RESUMO

The present study aims at designing a thermosensitive gel prepared from w1/o/w2 multiple microemulsions (MMEs) for the vaginal delivery of siRNA. The w1/o/w2 MMEs were prepared by two-step emulsifications: the first step was to prepare primary emulsions (w1/o) by low energy emulsification (LEE); the second step was to obtain stable w1/o/w2 MMEs by self-emulsifying. An extensive formulation optimization process was undertaken. The final w1/o/w2 MMEs could be formed in ddH2O, phosphate buffer solution (PBS, pH 7.4) and 1640 culture media with diameter size about 166.5 ± 13.1, 271.0 ± 11.1 and 278.7 ± 12.1 nm respectively. The release rates of siRNA from solutions, MMEs and MMEs-gels were completed within 2 h, 6 h and13 h respectively. The transfection efficiency of MMEs was confirmed both in vitro and in vivo. The relative target gene expressions of MMEs were 0.07 ± 0.05% vs. 0.37 ± 0.06% in Hela cells against Lipofectamine2000® and 1.88% ± 0.00% vs. 9.65% ± 0.02% in mouse vaginal mucosa against PEI. Good biocompatibility of MMEs was verified by cytotoxicity and pathological studies. Overall, our results indicated the potential of the MMEs-gel system for the vaginal delivery of siRNA.


Assuntos
Administração Intravaginal , Sistemas de Liberação de Medicamentos/métodos , Emulsões/administração & dosagem , Microesferas , Ácidos Nucleicos/administração & dosagem , Tensoativos/administração & dosagem , Animais , Emulsões/síntese química , Emulsões/metabolismo , Feminino , Géis , Células HeLa , Humanos , Camundongos , Ácidos Nucleicos/síntese química , Ácidos Nucleicos/metabolismo , Distribuição Aleatória , Tensoativos/síntese química , Tensoativos/metabolismo , Água/administração & dosagem , Água/química , Água/metabolismo
13.
Drug Deliv ; 26(1): 147-157, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30822171

RESUMO

Hypoxic pulmonary vasoconstriction (HPV) is a well-characterized vascular response to low oxygen pressures and is involved in life-threatening conditions such as high-altitude pulmonary edema (HAPE) and pulmonary arterial hypertension (PAH). While the efficacy of oral therapies can be affected by drug metabolism, or dose-limiting systemic toxicity, inhaled treatment via pressured metered dose inhalers (pMDI) may be an effective, nontoxic, practical alternative. We hypothesized that a stable water-in-perfluorooctyl bromide (PFOB) emulsion that provides solubility in common pMDI propellants, engineered for intrapulmonary delivery of pulmonary vasodilators, reverses HPV during acute hypoxia (HX). Male Sprague Dawley rats received two 10-min bouts of HX (13% O2) with 20 min of room air and drug application between exposures. Treatment groups: intrapulmonary delivery (PUL) of (1) saline; (2) ambrisentan in saline (0.1 mg/kg); (3) empty emulsion; (4) emulsion encapsulating ambrisentan or sodium nitrite (NaNO2) (0.1 and 0.5 mg/kg each); and intravenous (5) ambrisentan (0.1 mg/kg) or (6) NaNO2 (0.5 mg/kg). Neither PUL of saline or empty emulsion, nor infusions of drugs prevented pulmonary artery pressure (PAP) elevation (32.6 ± 3.2, 31.5 ± 1.2, 29.3 ± 1.8, and 30.2 ± 2.5 mmHg, respectively). In contrast, PUL of aqueous ambrisentan and both drug emulsions reduced PAP by 20-30% during HX, compared to controls. IL6 expression in bronchoalveolar lavage fluid and whole lung 24 h post-PUL did not differ among cohorts. We demonstrate proof-of-concept for delivering pulmonary vasodilators via aerosolized water-in-PFOB emulsion. This concept opens a potentially feasible and effective route of treating pulmonary vascular pathologies via pMDI.


Assuntos
Sistemas de Liberação de Medicamentos/métodos , Emulsões/administração & dosagem , Fluorcarbonetos/administração & dosagem , Hipertensão Pulmonar/tratamento farmacológico , Edema Pulmonar/tratamento farmacológico , Água/administração & dosagem , Animais , Anti-Hipertensivos/administração & dosagem , Anti-Hipertensivos/metabolismo , Avaliação Pré-Clínica de Medicamentos/métodos , Emulsões/metabolismo , Fluorcarbonetos/metabolismo , Hipertensão Pulmonar/diagnóstico por imagem , Hipertensão Pulmonar/metabolismo , Masculino , Fenilpropionatos/administração & dosagem , Fenilpropionatos/metabolismo , Circulação Pulmonar/efeitos dos fármacos , Circulação Pulmonar/fisiologia , Edema Pulmonar/diagnóstico por imagem , Edema Pulmonar/metabolismo , Piridazinas/administração & dosagem , Piridazinas/metabolismo , Ratos , Ratos Sprague-Dawley , Resultado do Tratamento , Água/metabolismo
14.
Food Chem ; 287: 76-84, 2019 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-30857721

RESUMO

In this study, the succinic anhydride (SA) modified whey protein isolate (WPI) and whey protein hydrolysates (WPH) were prepared and characterized as novel emulsifiers. After the succinylation, the solubility, emulsibility, and water/oil binding capacity of WPI/WPH were improved. Emulsions with modified and unmodified WPI/WPH were prepared and characterized on rheological property, particle sizes, and droplet distribution using rheometer, zeta-sizer and confocal laser scanning microscopy. A simulated digestion system was utilized to monitor the gastrointestinal fate of emulsions and the bioaccessibility of curcumin loaded in emulsions. The final digestion extents of WPIE-10 and WPHE-10 (emulsions stabilized by modified WPI/WPH with 10% SA addition) were lower than those of WPIE-0 and WPHE-0 (emulsions stabilized by unmodified WPI/WPH). The order of curcumin bioaccessibility was WPIE-10 (79.64%) ≈ WPHE-10 (86.75%) > WPIE-0 (64.23%) ≈ WPHE-0 (60.62%). Our study provided valuable information about novel emulsifiers stabilizing delivery system to improve curcumin bioaccessibility.


Assuntos
Curcumina , Emulsões , Hidrolisados de Proteína , Ácido Succínico , Proteínas do Soro do Leite , Curcumina/química , Curcumina/metabolismo , Emulsificantes , Emulsões/química , Emulsões/metabolismo , Tamanho da Partícula , Hidrolisados de Proteína/química , Hidrolisados de Proteína/metabolismo , Solubilidade , Ácido Succínico/química , Ácido Succínico/metabolismo , Proteínas do Soro do Leite/química , Proteínas do Soro do Leite/metabolismo
15.
Eur J Drug Metab Pharmacokinet ; 44(4): 459-480, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-30771095

RESUMO

Curcumin is a promising therapeutic agent that exhibits manifold therapeutic activities. However, it is challenging to study curcumin as it exhibits poor aqueous solubility and low permeability and it is a substrate for P-glycoprotein (P-gp). It is readily metabolized in the body, but many active metabolites of curcumin have been identified that could also be exploited for therapy. Strategies for the oral bioenhancement of curcumin to leverage the potential of curcumin as a therapeutic molecule are discussed here in light of these challenges. A brief discussion of conventional bioenhancement strategies using cyclodextrin complexes, solid dispersions, and solid self-emulsifying drug delivery systems is given. However, the major focus of this review is the application of nano-based approaches to the bioenhancement of curcumin. A description of the main advantages of nanosystems is followed by a detailed review of various nanosystems of curcumin, including nanosuspensions and various carrier-based nanosystems. Each nanosystem considered here is first briefly introduced, and then studies of the nanosystem containing curcumin are discussed. Lipid-based systems including liposomes and solid lipid nanoparticles, microemulsions, self-microemulsifying drug-delivery systems, nanoemulsions, and polymeric nanoparticles-which are widely explored-are dealt with in detail. Other miscellaneous systems discussed include inorganic nanoparticles, micelles, solid nanodispersions, phytosomes, and dendrimers. The possibility of using intact nanoparticles to achieve the targeted oral delivery of curcumin and thus harness the benefits of this wonder nutraceutical is an exciting prospect.


Assuntos
Curcumina/química , Curcumina/metabolismo , Emulsões/química , Emulsões/metabolismo , Nanopartículas/química , Nanopartículas/metabolismo , Administração Oral , Animais , Disponibilidade Biológica , Química Farmacêutica/métodos , Sistemas de Liberação de Medicamentos/métodos , Humanos , Lipídeos/química
16.
J Agric Food Chem ; 67(5): 1521-1529, 2019 Feb 06.
Artigo em Inglês | MEDLINE | ID: mdl-30663308

RESUMO

The influence of plant-based (gum arabic and quillaja saponin) and animal-based (whey protein isolate, WPI) emulsifiers on the production and stability of vitamin E-fortified emulsions was investigated. Their impact on lipid digestibility and vitamin bioaccessibility was also studied utilizing an in vitro gastrointestinal tract. WPI and saponin produced smaller emulsions than gum arabic. All emulsions had good storage stability at room temperature (4 weeks, pH 7). Saponin- and gum arabic-emulsions were resistant to droplet aggregation from pH 2 to 8 because these emulsifiers generated strong electrosteric repulsion. WPI-coated droplets flocculated around pH 5 due to a reduction in charge near their isoelectric point. Lipid digestion was slower in saponin-emulsions, presumably because the high surface activity of saponins inhibited their removal by bile acids and lipase. Vitamin bioaccessibility was higher in WPI- than in saponin- or gum arabic-emulsions. This information may facilitate the design of more efficacious vitamin-fortified delivery systems.


Assuntos
Portadores de Fármacos/química , Composição de Medicamentos/métodos , Emulsificantes/química , Vitamina E/química , Disponibilidade Biológica , Sistemas de Liberação de Medicamentos , Estabilidade de Medicamentos , Emulsificantes/metabolismo , Emulsões/química , Emulsões/metabolismo , Trato Gastrointestinal/metabolismo , Goma Arábica/química , Concentração de Íons de Hidrogênio , Extratos Vegetais/química , Quillaja/química , Saponinas/química , Vitamina E/metabolismo , Água/química , Proteínas do Soro do Leite/química
17.
J Agric Food Chem ; 67(3): 927-934, 2019 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-30608158

RESUMO

To investigate how the fat crystal structure affects lipid in vitro digestibility, 30% palm stearin-in-water emulsions were prepared after storage at different temperatures (4, 25, and 37 °C) for 1 h, which consisted of different polymorphic forms, sizes, and quantities of fat crystals. The variation of particle size ( d4,3), zeta potential, and microstructure during the gastrointestinal digestion and the free fatty acid (FFA) released in small intestine phase were investigated. After oral and gastric digestion, all of the emulsions underwent partial or complete coalescence and flocculation. During intestinal digestion, the d4,3 and zeta potentials did not notably affect lipid digestion. The FFA-released assay results indicated that the lipid digestion extent decreased as the fat crystal size and content of the ß polymorph increased, and there was no obvious relationship between FFA release and fat crystal quantity or solid fat content (SFC). This study highlighted the crucial roles of fat crystal size and polymorphic form in regulating the digestion behavior of lipid-based O/W emulsions.


Assuntos
Emulsões/metabolismo , Ácidos Graxos não Esterificados/química , Trato Gastrointestinal/metabolismo , Água/química , Digestão , Ácidos Graxos não Esterificados/metabolismo , Trato Gastrointestinal/química , Humanos , Modelos Biológicos , Tamanho da Partícula , Água/metabolismo
18.
AAPS PharmSciTech ; 20(2): 63, 2019 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-30627930

RESUMO

Topical administration is a preferable choice for local anesthetic delivery. Microemulsions have shown great effectiveness for transdermal transport of lidocaine. However, fabrication of microemulsions containing highly concentrated lidocaine (10%) to provide an extended local anesthetic effect is still a challenge. This study investigated the feasibility of using microemulsions for transdermal delivery of a high dosage of lidocaine (10%). At first, eutectic mixtures by kneading lidocaine with thymol were tailored to form a lipophilic solution, then the mixtures were readily incorporated into the oil phase of microemulsions after addition of proper surfactants and cosurfactants. The physicochemical properties, the skin permeation, local anesthetic efficacy, and the irritation experiment of the developed microemulsions were evaluated. The optimum composition was as follows: 12% of ethyl oleate as oil phase, 28% of the mixed surfactant, and cosurfactant (polyoxyl 15 hydroxystearate and ethanol) and 60% of the aqueous phase. The average particle size was about 13 nm. The transmission electron microscope (TEM) studies revealed almost homogeneous spherical globules without aggregation. The Fourier-transform infrared spectroscopy (FTIR) results highlighted the drugs homogeneously dispersed in the microemulsions. In vitro skin permeation and in vivo anesthesia effect evaluation indicated that microemulsions can enhance and extend the anesthetic effect of lidocaine. The irritable results indicated that the microemulsions had the better biocompatibility and the negligible influence on the dermal. Therefore, incorporating the eutectic mixtures into microemulsions could be proposed as an attractive choice and a promising transdermal delivery strategy for the future topical anesthetic therapy.


Assuntos
Anestésicos Locais/administração & dosagem , Sistemas de Liberação de Medicamentos/métodos , Emulsões/administração & dosagem , Lidocaína/administração & dosagem , Absorção Cutânea/efeitos dos fármacos , Administração Cutânea , Administração Tópica , Anestésicos Locais/química , Anestésicos Locais/metabolismo , Animais , Emulsões/química , Emulsões/metabolismo , Lidocaína/química , Lidocaína/metabolismo , Camundongos , Técnicas de Cultura de Órgãos , Medição da Dor/efeitos dos fármacos , Medição da Dor/métodos , Tamanho da Partícula , Absorção Cutânea/fisiologia
19.
J Agric Food Chem ; 67(1): 452-462, 2019 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-30517000

RESUMO

Incorporation of fish oil containing ω-3 polyunsaturated fatty acids (PUFAs) into functional foods remains challenging. In this study, caseinate and glycoconjugates (CD6, CD40, CD70, CD100) of caseinate to dextrans of different molecular weights (D6, D40, D70, D100 kDa) were used to stabilize fish oil emulsions, and the impact on physicochemical stability and gastrointestinal fate was investigated. The glycoconjugate of CD6 exhibited significantly higher conjugation efficiency, lower surface hydrophobicity ( H0), and lower surface activity than other glycoconjugates. The glycoconjugate of CD70 displayed the best emulsifying activity and emulsion stability. Except CD6 stabilized emulsions, all other emulsions showed fine storage stability over 14 d at 22 ± 1 °C. The glycoconjugate stabilized emulsions exhibited significantly lower peroxide value (PV) ( P < 0.05) than that of the caseinate stabilized one. During in vitro gastrointestinal tract digestion, the glycation of caseinate with dextrans changed the ζ-potential, average particle size ( D32), and particle size distribution of the emulsions, which influenced flocculation and coalescence of droplets, as demonstrated by confocal microscopy. Caseinate after glycation with dextrans significantly retarded the release of free fatty acids from emulsions ( P < 0.05) during in vitro lipolysis. These results suggested that the dextrans attached to caseinate by glycation played a vital role in physicochemical stability and gastrointestinal fate of emulsions, mainly by its steric hindrance to effectively prevent flocculation and coalescence of droplets.


Assuntos
Caseínas/química , Dextranos/química , Óleos de Peixe/química , Óleos de Peixe/metabolismo , Trato Gastrointestinal/metabolismo , Glicoconjugados/química , Caseínas/metabolismo , Dextranos/metabolismo , Digestão , Emulsões/química , Emulsões/metabolismo , Glicoconjugados/metabolismo , Glicosilação , Humanos , Interações Hidrofóbicas e Hidrofílicas , Tamanho da Partícula
20.
Biomed Pharmacother ; 110: 181-189, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30469082

RESUMO

Turmeric is a well-known functional food exhibiting multiple biological activities in health and disease. However, low aqueous solubility and poor bioavailability limit its therapeutic potential. Herein, we investigated the utility of nanoemulsions as a carrier to improve the efficacy of turmeric. Compared with turmeric extract (TE), 5% TE-loaded nanoemulsion (TE-NE), which contains 20-fold lower curcumin content than TE, achieved similar inhibition of palmitate-induced lipotoxicity in HepG2 cells. Exposure of HepG2 cells to 5% TE-NE also suppressed the palmitate-induced accumulation of lipid vacuoles and reactive oxygen species comparably with TE, and was accompanied by decreased levels of sterol regulatory element-binding protein (SREBP)-1, peroxisome proliferator-activated receptor-γ2 (PPAR-γ2), cleaved caspase-3, and poly (ADP-ribose) polymerase (PARP). Consistent with these effects in HepG2 cells, oral administration of 5% TE-NE to mice fed a high fat diet (HFD) markedly suppressed lipid accumulation in liver, leading to a significant reduction in body weight and adipose tissue weight, equivalent to the effects observed with TE. Compared with TE, 5% TE-NE also equivalently inhibited the levels of SREBP-1, PPAR-γ2, cleaved caspase-3, and PARP in the liver of mice fed a HFD. Furthermore, TE and 5% TE-NE significantly improved serum lipid profiles in a similar manner. These observations indicate that nanoemulsions can improve the efficacy of turmeric, thereby eliciting more potent biological efficacy against palmitate- and high fat diet (HFD)-induced cellular damage.


Assuntos
Dieta Hiperlipídica/efeitos adversos , Emulsões/administração & dosagem , Nanopartículas/administração & dosagem , Obesidade/tratamento farmacológico , Palmitatos/administração & dosagem , Extratos Vegetais/administração & dosagem , Animais , Peso Corporal/efeitos dos fármacos , Peso Corporal/fisiologia , Sobrevivência Celular/efeitos dos fármacos , Sobrevivência Celular/fisiologia , Curcuma , Relação Dose-Resposta a Droga , Portadores de Fármacos/administração & dosagem , Portadores de Fármacos/farmacocinética , Emulsões/metabolismo , Células Hep G2 , Humanos , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Nanopartículas/metabolismo , Obesidade/etiologia , Obesidade/metabolismo , Palmitatos/farmacocinética , Extratos Vegetais/farmacocinética , Resultado do Tratamento
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA