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1.
J Agric Food Chem ; 68(4): 1126-1135, 2020 Jan 29.
Artigo em Inglês | MEDLINE | ID: mdl-31891261

RESUMO

Phenylpropanoid (PPPN) compounds are widely used in agriculture, medical, food, and cosmetic industries because of their multiple bioactivities. Alternaria sp. MG1, an endophytic fungus isolated from grape, is a new natural source of PPPNs. However, the PPPN biosynthesis pathway in MG1 tends to be suppressed under normal growth conditions. Starvation has been reported to stimulate the PPPN pathway in plants, but this phenomenon has not been well studied in endophytic fungi. Here, metabolomics analysis was used to examine the profile of PPPN compounds, and quantitative reverse transcription-polymerase chain reaction was used to detect the expression of key genes in the PPPN biosynthesis pathway under starvation conditions. Starvation treatment significantly increased the accumulation of shikimate and PPPN compounds and upregulated the expression of key genes in their biosynthesis pathways. In addition to previously reported PPPNs, sinapate, 4-hydroxystyrene, piceatannol, and taxifolin were also detected under starvation treatment. These findings suggest that starvation treatment provides an effective way to optimize the production of PPPN compounds and may permit the investigation of compounds that are undetectable under normal conditions. Moreover, the diversity of its PPPNs makes strain MG1 a rich repository of valuable compounds and an extensive genetic resource for future studies.


Assuntos
Alternaria/metabolismo , Endófitos/metabolismo , Vitis/metabolismo , Vitis/microbiologia , Alternaria/genética , Alternaria/isolamento & purificação , Vias Biossintéticas , Ácidos Cumáricos/metabolismo , Endófitos/genética , Endófitos/isolamento & purificação , Proteínas Fúngicas/genética , Proteínas Fúngicas/metabolismo , Metabolômica , Fenóis/metabolismo , Quercetina/análogos & derivados , Quercetina/biossíntese , Metabolismo Secundário , Estilbenos/metabolismo
2.
Syst Appl Microbiol ; 43(1): 126025, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31704194

RESUMO

The main goal of the study was to determine the structure of endophytic bacteria inhabiting different parts (endosperm, germ, roots, coleoptiles, and leaves) of two wheat species, Triticum aestivum L. (cv. 'Hondia') and Triticum spelta L. (cv. 'Rokosz'), in order to provide new knowledge about the stability and/or changeability of the core microbiome in different plant organs. The endophytic core microbiome is associated with plants throughout their whole life cycle; however, plant organs can determine the actual endophytic community. Therefore, next generation sequencing with MiSeq Illumina technology was applied to identify the endophytic microbiome of T. aestivum and T. spelta. Bioinformatic analyses were performed with the use of the DADA2(1.8) package and R software (3.5.1). It was demonstrated that wheat, which is an important crop plant, was associated with beneficial endophytic bacteria inside the endosperms, germs, roots, leaves, and coleoptiles. Importantly, for the first time, biodiversity was recognized in the coleoptiles of the investigated wheat species. Flavobacterium, Pseudomonas and Janthinobacterium were shown to be common genera for both tested wheat cultivars. Among them, Pseudomonas was found to be the only endophytic genus accompanying both wheat species from the endosperm stage to the development of the leaf. Paenibacillus was recognized as a core genus for the 'Hondia' cv., whereas Pedobacter and Duganella constituted the core microbiome in the 'Rokosz' cv. In addition, the first insight into the unique and yet unrecognized endophytic microbiome of T. spelta is presented.


Assuntos
Microbiota , Triticum/microbiologia , Bactérias/classificação , Bactérias/genética , Bactérias/isolamento & purificação , Biodiversidade , Endófitos/classificação , Endófitos/genética , Endófitos/isolamento & purificação , Microbiota/genética , Especificidade de Órgãos , Estruturas Vegetais/microbiologia , Especificidade da Espécie , Triticum/genética
3.
Int J Syst Evol Microbiol ; 70(1): 481-486, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31613744

RESUMO

A Gram-staining-positive, aerobic, rod-shaped, endospore-forming bacterium, designated strain M5HDSG1-1T, was originally isolated from a surface-sterilized root of Taxus chinensis (Pilger) Rehd. in Guizhou, PR China. This bacterium was tested by a polyphasic approach to determine its taxonomic position. A 16S rRNA gene-based phylogenetic analysis revealed that M5HDSG1-1T had the greatest similarity to the type strain of Bacillus nealsonii DSM 15077T (99.1 %). The average nucleotide identity values between M5HDSG1-1T and Bacillus nealsonii DSM 15077T and Bacillus circulans NBRC 13626T were 73.3 and 72.8 %, respectively. The digital DNA-DNA hybridization values between M5HDSG1-1T and Bacillus nealsonii DSM 15077T and Bacillus circulans NBRC 13626T were 20.1 and 20.6 %, respectively, which were below the recommended thresholds. M5HDSG1-1T grew at a pH range of 6.0-12.0 (optimum, 7.0-8.0), at temperatures between 10 and 45 °C (optimum, 30 °C) and at 0-2 % (w/v) NaCl (optimum, 1 %). Neither substrate nor aerial mycelia was formed, and no diffusible pigments were observed on the media tested. The predominant isoprenoid quinone was menaquinone-7 (MK-7). The major fatty acids were anteiso-C15 : 0, and iso-C15 : 0. The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified aminophospholipid and an unidentified phospholipid. The DNA G+C content was 37.5 mol%. According to the phylogeneic, phenotypic and chemotaxonomic data, M5HDSG1-1T was clearly distinguishable from other species with validly published names in the genus Bacillus and should therefore be classified as representing a novel species, and we suggest the name Bacillus taxi sp. nov. The type strain is M5HDSG1-1T (=JCM 33117T=CGMCC 1.13668T).


Assuntos
Bacillus/classificação , Filogenia , Raízes de Plantas/microbiologia , Taxus/microbiologia , Bacillus/isolamento & purificação , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Endófitos/classificação , Endófitos/isolamento & purificação , Ácidos Graxos/química , Hibridização de Ácido Nucleico , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Vitamina K 2/análogos & derivados , Vitamina K 2/química
4.
Arch Microbiol ; 202(2): 247-255, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31602502

RESUMO

Many researchers proved that plant endophytes manage successful issues to synthesize active chemicals within plant cells. These bioactive compounds might support a range of plant defense mechanism against many pathogenic microorganisms. In this study, a total of 22 isolates representing 21 fungal species belonging to 15 fungal genera in addition to one variety were isolated and identified for the first time from Euphorbia geniculate plants. The genus Aspergillus was the most common fungus isolated from the studied plant. The fungus Isaria feline was recorded in both leaves and stem, while Aspergillus flavus, A. ochraceus, A. terreus var. terreus, Emercilla nidulans var. acristata, Macrophomina phaseolina colonized both stem and root. The isolated fungi showed antagonistic activities against six strains of plant pathogenic fungi viz., Eupenicillium brefeldianum, Penicillium echinulatum, Alternaria phragmospora, Fusarium oxysporum, Fusarium verticilloid, and Alternaria alternata in dual culture assay. The highest antagonistic activity fungal species (Aspergillus flavus, A. fumigatus, and Fusarium lateritium) and the lowest (Cladosprium herbarum, F. culomrum, and Sporotrichum thermophile) showed twining in their secondary metabolites especially terpens and alkaloids with that of their host E. geniculata. Three concentrations of (0.5, 1.0, and 2.0 mg/ml) of these secondary metabolites extracted by ethyl acetate and n-butanol from the above six endophytic fungal species were tested against three pathogenic fungi isolated from infected tomato plant (E. brefeldianum-EBT-1, P. echinulatum-PET-2, and A. phragmospora-APT-3), whereas these pathogens showed promising sensitivity to these fungal secondary metabolites. In conclusion, this is the first report on the isolation of endophytic fungi from E. geniculata and evaluation of their antifungal activity.


Assuntos
Antibiose/fisiologia , Endófitos/química , Euphorbia/microbiologia , Fungos/química , Plantas Medicinais/microbiologia , Antifúngicos/isolamento & purificação , Endófitos/isolamento & purificação , Fungos/isolamento & purificação , Fungos/metabolismo , Folhas de Planta/microbiologia
5.
Int J Syst Evol Microbiol ; 70(1): 220-227, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31535966

RESUMO

Strain EAR18T was isolated as an endophyte from the roots of a halophyte plant, Arthrocnemum macrostachyum, growing in the Odiel marshes (Huelva, Spain). Cells of strain EAR18T were Gram- stain-negative, motile, non-spore-forming aerobic rods. It grew optimally on tryptic soy agar supplemented with 2.5 % NaCl (w/v), at pH 7 and 30 °C for 48 h. It tolerated NaCl from 0 to 25 % (w/v). It presented Q9 as the major quinone and C19 : 0 cyclo ω8c, summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and C16 : 0 as the predominant fatty acids. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and four unidentified phospholipids. The results of phylogenetic analysis based on 16S rRNA gene sequences revealed that strain EAR18T formed a well-supported clade with species Halomonas zincidurans B6T and Halomonas xinjiangensis TRM 0175T (similarities of 98.3 and 96.1 % respectively). Furthermore, digital DNA-DNA hybridization analysis resulted in values of 20.4 % with H. xinjiangensis TRM 0175T and 35.50 % with H. zincidurans B6T, and ANIb/ANIm results in values of 73.8 %/84.2 % with H. xinjiangensis TRM 0175T and 86.8 %/89.4 % with H. zincidurans B6T. Based on phylogeny and differential phenotypic properties in comparison with its closest related species, strain EAR18T is suggested to represent a new species in the genus Halomonas, for which the name Halomonas radicis sp. nov. is proposed. The type strain is EAR18T (=CECT 9077T=LMG 29859T). The whole genome was sequenced, and it had a total length of 4.6 Mbp and a G+C content of 64.9 mol%.


Assuntos
Chenopodiaceae/microbiologia , Halomonas/classificação , Filogenia , Raízes de Plantas/microbiologia , Áreas Alagadas , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Endófitos/classificação , Endófitos/isolamento & purificação , Ácidos Graxos/química , Halomonas/isolamento & purificação , Hibridização de Ácido Nucleico , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Plantas Tolerantes a Sal/microbiologia , Análise de Sequência de DNA , Espanha
6.
J Agric Food Chem ; 68(1): 185-192, 2020 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-31815467

RESUMO

Phytopathogenic fungi have been considered as being an enormous threat in the agricultural system. In our search of new antifungal natural products, nine new halogenated cyclopentenones, bicolorins A-I (1-3, and 5-10), along with three known cyclopentenones (4, 11, and 12) were isolated from the endophytic fungus Saccharicola bicolor of Bergenia purpurascens by the one strain-many compounds strategy. Their structures and absolute configurations were elucidated based on extensive spectroscopic analysis, X-ray crystallographic analysis, and time-dependent density functional theory-equivalent circulating density calculations. Compounds 1-12 showed antifungal activities against five phytopathogenic fungi (Uromyces viciae-fabae, Pythium dissimile, Gibberella zeae, Aspergillus niger, and Sclerotinia sclerotiorum). Especially, bicolorins B and D (2 and 5) exhibited strong antifungal activities against P. dissimile with the MIC values of 6.2 and 8.5 µg/mL, respectively, compared with the positive control cycloheximide (MIC of 8.6 µg/mL). Additionally, bicolorin D was proven to be potently antifungal against S. sclerotiorum in vitro and in vivo. This work provides an effective strategy for searching antifungal candidate agents.


Assuntos
Ascomicetos/química , Ciclopentanos/química , Ciclopentanos/farmacologia , Endófitos/química , Fungicidas Industriais/química , Fungicidas Industriais/farmacologia , Saxifragaceae/microbiologia , Ascomicetos/isolamento & purificação , Ascomicetos/metabolismo , Ciclopentanos/metabolismo , Endófitos/isolamento & purificação , Endófitos/metabolismo , Fungos/efeitos dos fármacos , Fungos/crescimento & desenvolvimento , Fungicidas Industriais/metabolismo , Halogenação , Estrutura Molecular , Doenças das Plantas/microbiologia
7.
World J Microbiol Biotechnol ; 35(12): 182, 2019 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-31728757

RESUMO

Endophytes improve the host performance in areas of high plant endemicity. Paullinia cupana is an Amazonia plant species of economic and social importance due to the high caffeine concentration in its seeds. An interesting strategy to identify endophytic microorganisms with potential biotechnological application is to understand the factors that influence the endophytic community to rationalize the host management programs. We used the next-generation sequencing for bacterial 16S rRNA gene to examine how the P. cupana organ, genotype, and geographic location influenced its endophytic bacterial community. We obtained 1520 operational taxonomic units (OTUs) distributed in 19 phyla, 32 classes, 79 orders, 114 families and 174 genera. The P. cupana roots and leaves were specifically colonized by the bacterial genera Acidothermus and Porphyromonas, respectively, with high relative frequency. The plant organ type influenced the endophytic community's richness, diversity, OTUs composition, relative abundance of phyla and genera, and genera interaction network. However, the host plant genotype and geographic location influenced the composition and interaction among genera in the network analysis. Prevotella is a super-generalist genus in the interaction network of endophytic bacteria of P. cupana. This study revealed endophytic bacterial groups of importance to P. cupana and stressed that the host plant organ modulates the structure and interactions within this community. Our results indicated that the microbial community adapted to colonize P. cupana by adjusting to its composition and interaction network. The isolation of abundant and super-generalist bacterial genera shall help to examine their functionality to the composition and fitness of the endophytic community of P. cupana.


Assuntos
Bactérias/classificação , Bactérias/isolamento & purificação , Ecologia , Endófitos/classificação , Endófitos/isolamento & purificação , Paullinia/microbiologia , Bactérias/genética , Brasil , DNA Bacteriano/isolamento & purificação , Endófitos/genética , Genótipo , Sequenciamento de Nucleotídeos em Larga Escala , Interações Microbianas , Microbiota/genética , Filogenia , Folhas de Planta/microbiologia , Raízes de Plantas/microbiologia , RNA Ribossômico 16S/genética , Sementes/microbiologia
8.
J Agric Food Chem ; 67(43): 11994-12001, 2019 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-31618578

RESUMO

Bioactivity-guided isolation of the endophytic fungus Fusarium sambucinum TE-6L residing in Nicotiana tabacum L. led to the discovery of two new angularly prenylated indole alkaloids (PIAs) with pyrano[2,3-g]indole moieties, amoenamide C (1) and sclerotiamide B (2), and four known biosynthetic congeners (3-6). Their structures were determined by comprehensive spectroscopic techniques, electronic circular dichroism (ECD), and X-ray diffraction. Compound 1 containing the bicyclo[2.2.2]diazaoctane core and indoxyl unit is rarely reported. All the compounds were evaluated for their antimicrobial and insecticidal activities. Notably, compounds 1-3 showed potent inhibitory effects against three human- and one plant-pathogenic bacterium, and seven plant-pathogenic fungi. Compounds 2-4 also exhibited remarkable larvicidal activity against first instar larvae of the cotton bollworm Helicoverpa armigera with mortality rates of 70.2%, 83.2%, and 70.5%, respectively. Further toxicity tests on zebrafish embryos were performed to evaluate the potential toxicity of PIAs. Of significance was that compound 3 in particular exhibited the highest activities but the lowest effects on the hatching of embryos among all the compounds. This study provides a basis for understanding developmental toxicity of PIAs exposure to zebrafish embryos, and also indicates the potential environmental risks of other natural compounds exposure in the aquatic ecosystem.


Assuntos
Anti-Infecciosos/química , Endófitos/química , Fusarium/química , Alcaloides Indólicos/química , Inseticidas/química , Animais , Anti-Infecciosos/metabolismo , Anti-Infecciosos/farmacologia , Bactérias/efeitos dos fármacos , Endófitos/isolamento & purificação , Fungos/efeitos dos fármacos , Fusarium/isolamento & purificação , Alcaloides Indólicos/metabolismo , Alcaloides Indólicos/farmacologia , Inseticidas/metabolismo , Inseticidas/farmacologia , Larva/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Estrutura Molecular , Mariposas/efeitos dos fármacos , Tabaco/microbiologia , Peixe-Zebra/embriologia
9.
Zhongguo Zhong Yao Za Zhi ; 44(15): 3213-3220, 2019 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-31602874

RESUMO

A total of 27 endophytic fungal strains were isolated from Huperzia serrata,which were richly distributed in the stems and leaves while less distributed in roots. The 27 strains were identified by Internal Transcribed Spacer( ITS) r DNA molecular method and one of the strains belongs to Basidiomycota phylum,and other 26 stains belong to 26 species,9 general,6 families,5 orders,3 classes of Ascomycota Phylum. The dominant strains were Colletotrichum genus,belonging to Glomerellaceae family,Glomerellales order,Sordariomycetes class,Ascomycota Phylum,with the percentage of 48. 15%. The inhibitory activities of the crude extracts of 27 endophytic fungal strains against acetylcholinesterase( ACh E) and nitric oxide( NO) production were evaluated by Ellman's method and Griess method,respectively. Crude extracts of four fungi exhibited inhibitory activities against ACh E with an IC50 value of 42. 5-62. 4 mg·L~(-1),and some fungi's crude extracts were found to inhibit nitric oxide( NO) production in lipopolysaccharide( LPS)-activated RAW264. 7 macrophage cells with an IC50 value of 2. 2-51. 3 mg·L~(-1),which indicated that these fungi had potential anti-inflammatory activities.The chemical composition of the Et OAc extract of endophytic fungus HS21 was also analyzed by LCMS-IT-TOF. Seventeen compounds including six polyketides,four diphenyl ether derivatives and seven meroterpenoids were putatively identified.


Assuntos
Ascomicetos/química , Ascomicetos/classificação , Huperzia/microbiologia , Acetilcolinesterase , Animais , Anti-Inflamatórios/isolamento & purificação , Anti-Inflamatórios/farmacologia , Ascomicetos/isolamento & purificação , Inibidores da Colinesterase/isolamento & purificação , Inibidores da Colinesterase/metabolismo , Endófitos/classificação , Endófitos/isolamento & purificação , Camundongos , Células RAW 264.7
10.
Int J Syst Evol Microbiol ; 69(12): 3939-3945, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31526457

RESUMO

Strain TE3T, an endophytic plant growth promoting bacterium, was isolated from wheat (Triticumturgidum subsp. durum) sampled in the Yaqui Valley, Mexico. Biochemical, phenotypic and genotypic approaches were used to clarify the taxonomic affiliation of this strain. Based on analysis of its full-length 16S rRNA gene, strain TE3T was assigned to the genus Bacillus (similarity ≥98.7 %). This finding was supported by morphological and metabolic characteristics, such as rod shape, strictly aerobic metabolism, spore formation, Gram-positive staining, catalase-positive activity, reduction of nitrate to nitrite, starch and casein hydrolysis, growth in presence of lysozyme and 2 % NaCl, citrate utilization, growth pH from 6.0 to 8.0, and acid and indole production from glucose and tryptophan, respectively. The whole-genome phylogenetic relationship showed that TE3T formed an individual clade with Bacillus tequilensis KCTC 13622T, distant from that generated by all Bacillus subtilis subspecies. The maximum values for average nucleotide identity and in silico DNA-DNA hybridization were 93.85 and 54.30 %, respectively, related to Bacillus subtilissubsp. inaquosorum KCTC 13429T. Analysis of its fatty acid content showed the ability of strain TE3T to bio-synthetize fatty acids that are not present in closely related Bacillus species, such as C12 : 0, C12 : 0 2OH, C12 : 0 3OH, C17 : 0, iso-C17 : 0 3OH and C18 : 1ω9c. These results provide evidence that strain TE3T is a novel species of the genus Bacillus, for which the name Bacilluscabrialesii sp. nov. is proposed. The type strain of Bacilluscabrialesii is TE3T (CM-CNRG TB54T=CCStamb A1T).


Assuntos
Bacillus/classificação , Filogenia , Triticum/microbiologia , Bacillus/isolamento & purificação , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Endófitos/classificação , Endófitos/isolamento & purificação , Ácidos Graxos/química , México , Hibridização de Ácido Nucleico , RNA Ribossômico 16S/genética , Análise de Sequência de DNA
11.
Microbiol Res ; 229: 126328, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31521946

RESUMO

Endophytic bacteria-based biocontrol is regarded as a potential plant disease management strategy. Present study analyzed the diversity of mulberry endophytic bacteria basing on a culture-dependent approach and further evaluated their antimicrobial and plant growth-promoting (PGP) activities. A total of 608 cultivable endophytic bacteria, belonging to 4 phyla and 36 genera, were isolated from four mulberry cultivars having different resistance to sclerotiniosis in three seasons. Taxonomic compositional analysis results showed that Proteobacteria, Firmicutes, and Actinobacteria were the three dominant bacterial phyla in all communities, with the representative genera Pantoea, Bacillus, Pseudomonas, Curtobacterium, and Sphingomonas. Diversity analysis results indicated that the diversity of winter community was higher than that of spring or autumn, and higher diversities were detected in the resistant cultivar communities compared with the susceptible cultivar. Antagonism assays results showed that 33 isolates exhibited strong and stable activity against three phytopathogens which are Sclerotinia sclerotiorum, Botrytis cinerea, and Colletotrichum gloeosporioide. Eight endophytic bacteria were selected out from 33 antagonists based on the evaluation of antagonistic and PGP activities. Furthermore, pot experiment results revealed that all the 8 tested endophytes stimulated the growth of mulberry seedlings at different levels, and Bacillus sp. CW16-5 exhibited the highest promotion capacity, which the shoot length and the root fresh weight were increased by 83.37% and 217.70%, respectively. Altogether, present study revealed that mulberry harbors a large amount of diverse cultivable endophytic bacteria and they also serve as novel sources of beneficial bacteria and bioactive metabolites.


Assuntos
Antibiose , Bactérias/isolamento & purificação , Biodiversidade , Endófitos/isolamento & purificação , Morus/microbiologia , Ascomicetos/fisiologia , Bactérias/classificação , Bactérias/genética , Fenômenos Fisiológicos Bacterianos , Botrytis/fisiologia , Colletotrichum/fisiologia , Endófitos/classificação , Endófitos/genética , Endófitos/fisiologia , Morus/crescimento & desenvolvimento , Doenças das Plantas/microbiologia
12.
J Agric Food Chem ; 67(38): 10685-10693, 2019 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-31479251

RESUMO

Theanine is the most abundant non-protein amino acid in Camellia sinensis, but it is not known how a tea plant accumulates such high levels of theanine. The endophyte isolated from in vitro grown plantlets of C. sinensis cultivars was identified as Luteibacter spp., showing strong biocatalytic activity for converting both glutamine and ethylamine to theanine. Theanine was secreted outside of the bacteria. The endophyte isolated from in vitro plantlets of Camellia oleifera cultivar was identified as Bacillus safensis and did not convert glutamine and ethylamine to theanine. Enzymatic assays in vitro indicated that γ-glutamyltranspeptidases rCsEGGTs from the endophyte Luteibacter strains converted glutamine and ethylamine to theanine at higher rates than rCsGGTs from C. sinensis. This is the first report on theanine biosynthesis by an endophyte from C. sinensis, which provides a new pathway to explore the mechanism of theanine biosynthesis in C. sinensis and the interactions between an endophyte and tea plants.


Assuntos
Bactérias/metabolismo , Camellia sinensis/microbiologia , Endófitos/metabolismo , Glutamatos/metabolismo , Bactérias/classificação , Bactérias/genética , Bactérias/isolamento & purificação , Camellia sinensis/química , Camellia sinensis/classificação , Endófitos/classificação , Endófitos/genética , Endófitos/isolamento & purificação , Etilaminas/metabolismo , Glutamina/metabolismo , Folhas de Planta/química , Folhas de Planta/microbiologia
13.
J Agric Food Chem ; 67(38): 10646-10652, 2019 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-31479255

RESUMO

A new monosesquiterpene diacetylgliocladic acid (1), a new dimeric sesquiterpene divirensol H (9), and two exceptionally novel trimeric sesquiterpene trivirensols A and B (11 and 12), together with another eight known congeners, were purified from an endophytic fungus Trichoderma virens FY06, derived from Litchi chinensis Sonn. whose fruit is a delicious and popular food. All of them were identified by comprehensive spectroscopic analysis, combined with biosynthetic considerations. Trivirensols A and B are unprecedented trimers of which three subunits are connected by two ester bonds of the sesquiterpene class. Relative to the positive control triadimefon, all the tested metabolites showed strong inhibitory activities against at least one phytopathogenic fungus among Penicillium italicum, Fusarium oxysporum, Fusarium graminearum, Colletotrichum musae, and Colletotrictum gloeosporioides. Notably, as metabolites of the endophytic fungus from L. chinensis, they all presented strong antifungal activities against C. gloeosporioides which causes anthracnose in L. chinensis.


Assuntos
Fungicidas Industriais/farmacologia , Litchi/microbiologia , Sesquiterpenos/farmacologia , Trichoderma/química , Colletotrichum/efeitos dos fármacos , Colletotrichum/crescimento & desenvolvimento , Endófitos/química , Endófitos/genética , Endófitos/isolamento & purificação , Endófitos/metabolismo , Frutas/microbiologia , Fungicidas Industriais/química , Fungicidas Industriais/metabolismo , Fusarium/efeitos dos fármacos , Fusarium/crescimento & desenvolvimento , Testes de Sensibilidade Microbiana , Doenças das Plantas/microbiologia , Sesquiterpenos/química , Sesquiterpenos/metabolismo , Trichoderma/genética , Trichoderma/isolamento & purificação
14.
J Agric Food Chem ; 67(38): 10660-10666, 2019 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-31479263

RESUMO

Two new indolyl diketopiperazines, gartryprostatins A and B (1 and 2), with an unusual 2,3-furan-fused pyrano[2,3-g]pyrrolo[1″,2″:4',5']pyrazino[1',2':1,5]pyrrolo[2,3-b]indole nucleus, along with a new naturally occurring compound (gartryprostatin C, 3) were isolated from the solid culture of Aspergillus sp. GZWMJZ-258, an endophyte from Garcinia multiflora (Guttiferae). The structures of compounds 1-3 were determined by nuclear magnetic resonance, mass spectrometry, Marfey's analysis of amino acids, and chemical calculation. Compounds 1-3 displayed selective inhibition on human FLT3-ITD mutant AML cell line, MV4-11, with IC50 values of 7.2, 10.0, and 0.22 µM, respectively.


Assuntos
Aspergillus/química , Dicetopiperazinas/farmacologia , Endófitos/química , Garcinia/microbiologia , Plantas Medicinais/microbiologia , Aspergillus/genética , Aspergillus/isolamento & purificação , Aspergillus/metabolismo , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Dicetopiperazinas/química , Dicetopiperazinas/metabolismo , Endófitos/genética , Endófitos/isolamento & purificação , Endófitos/metabolismo , Humanos , Espectroscopia de Ressonância Magnética , Espectrometria de Massas , Estrutura Molecular
15.
Microbiol Res ; 228: 126302, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31442862

RESUMO

Endophytic bacteria isolated from cactus were characterized and assessed for their capability to induce drought tolerance and growth promotion in tomato. A total of 191-bacteria representing 13-genera and 18-species were isolated from wild cactus, Euphorbia trigonas. Bacillus (58), Lysinibacillus (36), Enterobacter (29), Stenotrophomonas (18), Lelliottia (12) and Pseudomonas (12) were the most represented genera. 16S rDNA sequence (>1400-bp) comparison placed the bacterial isolates with Bacillus xiamenensis; Bacillus megaterium; Bacillus cereus; Bacillus amyloliquefaciens; Bacillus velezensis; Brevibacillus brevis; Lysinibacillus fusiformis; Enterobacter cloacae; Lelliottia nimipressuralis; Proteus penneri; Sphingobacterium multivorum; Klebsiella pneumoniae; Pseudomonas putida; Pseudomonas aeruginosa; Stenotrophomonas maltophilia; Citrobacter freundii; Chryseobacterium indologenes and Paracoccus sp. Bacillus xiamenensis was identified for the first time as plant endophyte. Upon bacterization, the endophytes triggered germination and growth promotion in tomato as indicated by 118 % and 52 % more root-biomass under drought-free and drought-induced conditions, respectively. Bacillus amyloliquefaciens CBa_RA37 and B. megaterium RR10 displayed broad spectrum endophytism in tomato. Bacterization of tomato with cactus endophyte showed altered oxidative status, stomatal and photosystem II functioning, internal leaf temperature and relative water content suggestive of physiological de-stressing from moisture stress. Activity of oxidative stress enzymes such as guaiacol peroxidase and catalase was also indicative of endophyte assisted de-stressing of tomato. Re-irrigation on 20-days of drought infliction showed 86.9% recovery of B. amyloliquefaciens CBa_RA37 primed tomato when non-primed plantlets succumbed. The cactus endophytic bacterial strain B. amyloliquefaciens CBa_RA37 showed promise for low-cost, efficient and environmentally friendly bio-inoculant technology to mitigate drought in arid zones of Asian and African continents.


Assuntos
Cactaceae/microbiologia , Secas , Endófitos/classificação , Endófitos/isolamento & purificação , Endófitos/fisiologia , Lycopersicon esculentum/crescimento & desenvolvimento , Lycopersicon esculentum/microbiologia , Desenvolvimento Vegetal , Aclimatação , Bacillus/classificação , Bacillus/genética , Bacillus/isolamento & purificação , Bacillus/fisiologia , Biomassa , Camarões , DNA Ribossômico/genética , Clima Desértico , Endófitos/genética , Filogenia , Folhas de Planta , Raízes de Plantas , RNA Ribossômico 16S/genética , Rifamicinas/farmacologia , Análise de Sequência , Microbiologia do Solo , Estresse Fisiológico
16.
J Appl Microbiol ; 127(5): 1468-1478, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31403229

RESUMO

AIMS: Medicinal plant-associated endophytic fungi are important sources of precious bioactive compounds, contributing more than 80% of the natural drugs for various ailments. The present study was aimed at evaluating the anticancer activity of the crystallized compound alternariol methyl ether (AME) against hepatocellular carcinoma (HCC) both in vitro and in vivo from an endophytic fungus residing in the medicinal plant Vitex negundo. METHODS AND RESULTS: The secondary metabolites from the endophytic fungus Alternaria alternata MGTMMP031 were isolated. Purification and characterization of the compound was performed and the potential compound was identified as AME. The crystal structure of AME was unambiguously confirmed by X-ray analysis. AME has been checked for its antibacterial and anticancer properties which showed its effectiveness against various bacteria and demonstrated marked anti-proliferative activity against the human HCC cells (HUH-7) both in vitro and in vivo. Mode of actions included cell cycle arrest, reducing the level of markers enzymes of liver cancer and preventing tumour growth. CONCLUSIONS: Alternariol methyl ether acts as a potential therapeutic target against HCC. The compound was isolated and the crystal structure was obtained for the first time from the endophytic fungus A. alternata MGTMMP031. In the present study, the crystallized structure of AME was obtained by slow evaporation technique. It can be concluded that AME acts as a potential therapeutic target against HCC. SIGNIFICANCE AND IMPACT OF THE STUDY: Endophytic fungi residing in the medicinal plants have strong biological significance and bioactive compounds from these fungi provide better therapeutic targets against diseases.


Assuntos
Alternaria/química , Antineoplásicos/química , Antineoplásicos/farmacologia , Endófitos/química , Lactonas/isolamento & purificação , Lactonas/farmacologia , Éteres Metílicos/química , Plantas Medicinais/microbiologia , Alternaria/isolamento & purificação , Alternaria/metabolismo , Antineoplásicos/isolamento & purificação , Antineoplásicos/metabolismo , Carcinoma Hepatocelular/tratamento farmacológico , Carcinoma Hepatocelular/fisiopatologia , Pontos de Checagem do Ciclo Celular/efeitos dos fármacos , Cristalização , Endófitos/isolamento & purificação , Endófitos/metabolismo , Humanos , Lactonas/química , Lactonas/metabolismo , Neoplasias Hepáticas/tratamento farmacológico , Neoplasias Hepáticas/fisiopatologia , Éteres Metílicos/isolamento & purificação , Éteres Metílicos/farmacologia , Metabolismo Secundário
17.
Microbiol Res ; 228: 126299, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31422231

RESUMO

Wastewaters from textile dyeing industries represent an ecological concern, notably due to the known toxicity of azo dyes to the local microbiome and human health. Although physicochemical approaches are the rule for the treatment of industrial effluents, biological strategies such as enzyme-mediated dye destaining is a promising alternative. Notwithstanding a broad range of microorganisms, including fungi, algae, yeast, and bacteria, display dye-destaining properties, most of the literature has focused in ligninolytic fungi, leaving other classes of organisms somehow ignored. In this study, six endophytic strains isolated from Maytenus ilicifolia were studied for their destaining activity. The phylogenetic and morphological analysis allowed the identification of strain LGMF1504 as Neopestalotiopsis sp. LGMF1504 that decolorized several commercial dyes as the result of a mycelium-associated laccase. The enzyme expression was modulated by carbon and nitrogen content in the culture medium, it was weakly affected by the presence of aromatic compounds and metal ions while some common laccase mediators improved the destaining activity onto dye substrates. The best culture condition observed for laccase activity was a basic culture medium containing 5 g L-1 starch and 15 g L-1 ammonium tartrate. The laccase activity showed low substrate specificity and almost unaltered performance in a wide range of pH values and NaCl concentrations, suggesting the potential of Neopestalotiopsis sp. LGMF1504 for biodegradation approaches.


Assuntos
Corantes/metabolismo , Endófitos/metabolismo , Lacase/metabolismo , Micélio/metabolismo , Compostos Azo/toxicidade , Biodegradação Ambiental , Carbono , Corantes/toxicidade , DNA Fúngico , Endófitos/classificação , Endófitos/isolamento & purificação , Fungos/classificação , Fungos/genética , Fungos/isolamento & purificação , Fungos/metabolismo , Concentração de Íons de Hidrogênio , Metais , Tipagem de Sequências Multilocus , Nitrogênio , Filogenia , Cloreto de Sódio/análise , Indústria Têxtil , Águas Residuárias
18.
J Antibiot (Tokyo) ; 72(10): 779-782, 2019 10.
Artigo em Inglês | MEDLINE | ID: mdl-31267010

RESUMO

One new pentenoic acid derivative, named 1,1'-dioxine-2,2'-dipropionic acid (1) and a new natural product, named 2-methylacetate-3,5,6-trimethylpyrazine (2), along with six known compounds (3-8), were obtained from the Cladosporium sp. JS1-2, an endophytic fungus isolated from the mangrove Ceriops tagal collected in South China Sea. Their structures were elucidated by detailed analysis of comprehensive spectroscopic data, and the structure of 1 was further determined by X-ray diffraction analyses. 13C NMR chemical shifts of structure 2 was further determined by GIAO based 13C NMR chemical shifts calculations. Compounds 1-4 and 6 showed growth inhibition activities against newly hatched larvae of Helicoverpa armigera Hubner with the IC50 values ranging from 100 to 150 µg ml-1. Compounds 1, 2, 4, 6 and 7 showed moderate antibacterial activities against Staphylococcus aureus with the MIC values of 25.0, 12.5, 6.25, 1.25, and 6.25 µg ml-1, respectively.


Assuntos
Antibacterianos/isolamento & purificação , Produtos Biológicos/isolamento & purificação , Cladosporium/química , Inseticidas/isolamento & purificação , Animais , Antibacterianos/química , Antibacterianos/farmacologia , Bioensaio , Produtos Biológicos/química , Produtos Biológicos/farmacologia , China , Cladosporium/isolamento & purificação , Endófitos/química , Endófitos/isolamento & purificação , Concentração Inibidora 50 , Inseticidas/química , Inseticidas/farmacologia , Lepidópteros/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Estrutura Molecular , Rhizophoraceae/microbiologia , Análise Espectral , Staphylococcus aureus/efeitos dos fármacos
19.
J Environ Sci Health B ; 54(8): 693-701, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31271109

RESUMO

Di-n-butyl phthalate (DBP) is one of the primary PAEs (phthalate acid esters) pollutants. DBP can be absorbed by plants and threaten human health via the food chain. Some DBP-degrading bacteria have been successfully isolated from the environment (water, soil, etc.). However, only a few DBP-degrading plant endophytes have been isolated. In this study, an endophytic bacterium, Bacillus amyloliquefaciens subsp. strain JR20, which was found capable of degrading DBP, was isolated from garlic chive. We found that strain JR20 metabolized 89.74% of DBP at a 5 mg/L concentration within 4 d in liquid mineral salts medium (MSM). The optimized conditions for maximum removal of DBP were as follows: DBP concentration, 5 mg/L; pH, 7-8; temperature, 30-40 °C. The colonization of strain JR20 significantly improved the degradation rate of DBP in the roots, stems and leaves of leafy vegetables.


Assuntos
Bacillus amyloliquefaciens/metabolismo , Cebolinha-Francesa/microbiologia , Dibutilftalato/metabolismo , Verduras/microbiologia , Bacillus amyloliquefaciens/genética , Bacillus amyloliquefaciens/isolamento & purificação , Biodegradação Ambiental , Endófitos/isolamento & purificação , Endófitos/metabolismo , Poluentes Ambientais/metabolismo , Concentração de Íons de Hidrogênio , Folhas de Planta/metabolismo , Raízes de Plantas/metabolismo , Temperatura Ambiente , Verduras/metabolismo
20.
J Antibiot (Tokyo) ; 72(10): 752-758, 2019 10.
Artigo em Inglês | MEDLINE | ID: mdl-31324892

RESUMO

Two new diketopiperazines (1, 2), one new polyprenol (3), together with 19 known compounds (4-22) were obtained from the EtOAc extract of Bionectria sp. Y1085, an endophytic fungus isolated from the plant Huperzia serrata. Their structures were elucidated by extensive NMR and MS analysis. Bionectin D (1) is a rare diketopiperazine with a single methylthio substitution at the α-carbon of cyclized amino acid residue. The antibacterial activity of compounds was assayed against Escherichia coli, Staphylococcus aureus, and Salmonella typhimurium ATCC 6539, and some metabolites (1, 2, 10, 11, and 14) exhibited evident antibacterial activity.


Assuntos
Antibacterianos/isolamento & purificação , Dicetopiperazinas/isolamento & purificação , Endófitos/química , Hypocreales/química , Antibacterianos/química , Antibacterianos/farmacologia , Dicetopiperazinas/química , Dicetopiperazinas/farmacologia , Endófitos/isolamento & purificação , Escherichia coli/efeitos dos fármacos , Huperzia/microbiologia , Hypocreales/isolamento & purificação , Espectroscopia de Ressonância Magnética , Espectrometria de Massas , Testes de Sensibilidade Microbiana , Estrutura Molecular , Salmonella typhimurium/efeitos dos fármacos , Staphylococcus aureus/efeitos dos fármacos
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