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1.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi ; 36(6): 1038-1042, 2019 Dec 25.
Artigo em Chinês | MEDLINE | ID: mdl-31875380

RESUMO

Circular RNA (circRNA) is a type of single-stranded RNA that binds in a closed loop structure by covalent bond. It is highly expressed and has diverse functions in the eukaryotic transcriptome, and it also has the potential to regulate the process of cell differentiation. Stem cells are important seed cells and common research tools in the field of tissue engineering, which have multi-directional differentiation potential and low immunogenicity. Its clinical application for the treatment of diseases has broad prospects, and the research on their differentiation mechanism has gradually penetrated to the molecular level. A number of studies have shown that circRNA participates in stem cell differentiation and plays a key role through a variety of pathways. This article focuses on the expression changes of circRNA during stem cell differentiation and its research advancement in regulating the differentiation mechanism of various stem cells. The review also prospects its possible role in tissue regeneration and repair, in order to further study the molecular mechanism of circRNA involved in stem cell differentiation and provide ideas for clinical practice of stem cells in biomedical engineering.


Assuntos
Diferenciação Celular , Engenharia Tecidual , RNA , Células-Tronco , Transcriptoma
2.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi ; 36(6): 1055-1059, 2019 Dec 25.
Artigo em Chinês | MEDLINE | ID: mdl-31875383

RESUMO

As a temporary skin substitute, the dressings can protect the wound, stop bleeding, prevent infection and contribute to wound healing. According to the characteristics of the materials, wound dressings can be classified into traditional wound dressings, interactive dressings, bioactive dressings, tissue engineering dressings and smart dressings, etc. Different dressings have different characteristics, and some products have been widely used in clinic. Recently nanomaterials and three-dimensional bio-printing technology have significantly improved the performance of wound dressings. Future dressings will be developed from single function to multi-function composite, and integrated into an intelligent one. This paper reviews the current research progress and future development prospects of wound dressings.


Assuntos
Bandagens , Cicatrização , Pele Artificial , Engenharia Tecidual
3.
Zhonghua Shao Shang Za Zhi ; 35(12): 887-890, 2019 Dec 20.
Artigo em Chinês | MEDLINE | ID: mdl-31877614

RESUMO

Dental stem cell is a kind of stem cell isolated from dental hard tissue or periodontal tissue, including dental pulp stem cell, stem cell from human exfoliated deciduous teeth, stem cell from root apical papilla, periodontal ligament stem cell, dental follicle progenitor cell, and so on. As seed cell, dental stem cell provides safe and efficient cell source for nerve tissue engineering research. The review aims to introduce the characteristics of these dental stem cells in promoting the regeneration and preparation of nerve and the clinical application.


Assuntos
Células-Tronco , Engenharia Tecidual , Diferenciação Celular , Saco Dentário , Humanos , Ligamento Periodontal , Regeneração
4.
Zhonghua Shao Shang Za Zhi ; 35(12): 890-893, 2019 Dec 20.
Artigo em Chinês | MEDLINE | ID: mdl-31877615

RESUMO

Nanometer zinc oxide has become a new hotspot in the research of tissue engineering materials due to its excellent antibacterial properties, biocompatibility, and anti-tumor properties. In this paper, the existing research results were summarized, generalized, and analyzed. The antibacterial mechanism of nanometer zinc oxide was discussed in depth. The antibacterial properties and advantages of the latest nanometer zinc oxide composite materials were introduced in detail. In this review, we made prospect of the future application of nanometer zinc oxide.


Assuntos
Antibacterianos/uso terapêutico , Engenharia Tecidual , Óxido de Zinco
5.
Pharm Res ; 37(1): 8, 2019 Dec 17.
Artigo em Inglês | MEDLINE | ID: mdl-31848830

RESUMO

PURPOSE: Microphysiological systems (MPS), also known as "organs-on-chips" or "tissue chips," leverage recent advances in cell biology, tissue engineering, and microfabrication to create in vitro models of human organs and tissues. These systems offer promising solutions for modeling human physiology and disease in vitro and have multiple applications in areas where traditional cell culture and animal models fall short. Recently, the National Center for Advancing Translational Sciences (NCATS) at the National Institutes of Health (NIH) and the International Space Station (ISS) U.S. National Laboratory have coordinated efforts to facilitate the launch and use of these MPS platforms onboard the ISS. Here, we provide an introduction to the NIH Tissue Chips in Space initiative and an overview of the coordinated efforts between NIH and the ISS National Laboratory. We also highlight the current progress in addressing the scientific and technical challenges encountered in the development of these ambitious projects. Finally, we describe the potential impact of the Tissue Chips in Space program for the MPS field as well as the wider biomedical and health research communities.


Assuntos
Engenharia Tecidual/métodos , Ausência de Peso , Animais , Humanos , Microfluídica , Voo Espacial , Estados Unidos
6.
J Biomed Nanotechnol ; 15(11): 2209-2215, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31847935

RESUMO

Fibrous scaffold could provide extracellular matrix (ECM) like structure and desired network for cell growth; however, the mechanical performance of this type uni-structured fibrous scaffold cannot meet the requirement of tissue formation. Therefore, new strategies are needed for form mechanical strength enhancement. In this study, we developed three dimensional double-network structured fibrous scaffold (3D DN-Fs) using self-assembly technology combined with electrospinning technology. Our 3D DN-Fs consists of two types of skeletons: the finer silk nanofibers which can mimic biocompatible ECM structure; and the larger skeletal fibrous layers can greatly improve the mechanical strength and cellular loading ability, and provide good nutrition and excreta delivery system for cell growth. Therefore, our 3D DN-Fs displayed excellent mechanical performance (more than 50% increment), biocompatibility, biodegradability, and a desirable microenvironment for cell growth. More importantly, cultured cells exhibited excellent viability and 3D growth. Our novel strategy greatly enhances the potential application of fibrous scaffold in the biomedical area, such as 3D cell culture and tissue engineering.


Assuntos
Proliferação de Células , Tecidos Suporte , Materiais Biocompatíveis , Matriz Extracelular , Nanofibras , Engenharia Tecidual
7.
Chem Soc Rev ; 48(23): 5564-5595, 2019 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-31670726

RESUMO

The design and applications of some inorganic two-dimensional (2D) nanomaterials such as graphene, graphyne, and borophene have been widely studied in recent years. Meanwhile, it has been noticed that self-assembling two-dimensional organic biomaterials (2DOBMs) including films, membranes, nanosheets, nanoribbons, grids, arrays, and lattices based on various biomolecules also exhibited promising structures, functions, and applications. The in-depth studies on the self-assembly formation, structural and functional tailoring of 2DOBMs open new avenues for the next generation of novel nanomaterials with adjustable structure and functions, which would further promote the applications of 2DOBMs in materials science, nanodevices, energy and environmental science, biomedicine, tissue engineering, and analytical science. In this review, we summarize important information on the basic principles to fabricate self-assembling 2DOBMs based on peptides, proteins, DNA, RNA, viruses, and other biopolymers. The potential strategies and techniques for tailoring and controlling the structures and functions of 2DOBMs are presented and discussed further. The function-specific biomedical applications of 2DOBMs in biosensors, biomimetic mineralization, cell growth, drug/gene delivery, and bioimaging are also highlighted.


Assuntos
Materiais Biocompatíveis/química , Animais , Técnicas Biossensoriais/métodos , DNA/química , Portadores de Fármacos/química , Nanoestruturas/química , Imagem Óptica , Polímeros/química , Proteínas/química , Engenharia Tecidual
8.
Adv Exp Med Biol ; 1174: 371-399, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31713206

RESUMO

Biomaterials play a critical role in regenerative strategies such as stem cell-based therapies and tissue engineering, aiming to replace, remodel, regenerate, or support damaged tissues and organs. The design of appropriate three-dimensional (3D) scaffolds is crucial for generating bio-inspired replacement tissues. These scaffolds are primarily composed of degradable or non-degradable biomaterials and can be employed as cells, growth factors, or drug carriers. Naturally derived and synthetic biomaterials have been widely used for these purposes, but the ideal biomaterial remains to be found. Researchers from diversified fields have attempted to design and fabricate novel biomaterials, aiming to find novel theranostic approaches for tissue engineering and regenerative medicine. Since no single biomaterial has been found to possess all the necessary characteristics for an ideal performance, over the years scientists have tried to develop composite biomaterials that complement and combine the beneficial properties of multiple materials into a superior matrix. Herein, we highlight the structural features and performance of various biomaterials and their application in regenerative medicine and for enhanced tissue engineering approaches.


Assuntos
Biomimética , Matriz Extracelular , Engenharia Tecidual , Materiais Biocompatíveis/química , Matriz Extracelular/química , Medicina Regenerativa , Tecidos Suporte/química , Tecidos Suporte/normas
9.
Adv Exp Med Biol ; 1174: 401-440, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31713207

RESUMO

The human body can be viewed as an organism consisting of a variety of cellular and non-cellular materials interacting in a highly ordered manner. Its complex and hierarchical nature inspires the multi-level recapitulation of the human body in order to gain insights into the inner workings of life. While traditional cell culture models have led to new insights into the cellular microenvironment and biological control in vivo, deeper understanding of biological systems and human pathophysiology requires the development of novel model systems that allow for analysis of complex internal and external interactions within the cellular microenvironment in a more relevant organ context. Engineering organ-on-chip systems offers an unprecedented opportunity to unravel the complex and hierarchical nature of human organs. In this chapter, we first highlight the advances in microfluidic platforms that enable engineering of the cellular microenvironment and the transition from cells-on-chips to organs-on-chips. Then, we introduce the key features of the emerging organs-on-chips and their proof-of-concept applications in biomedical research. We also discuss the challenges and future outlooks of this state-of-the-art technology.


Assuntos
Microfluídica , Engenharia Tecidual , Microambiente Celular , Humanos , Microfluídica/tendências , Modelos Biológicos , Técnicas de Cultura de Órgãos/tendências , Fisiologia/tendências , Engenharia Tecidual/tendências
10.
Zhonghua Yan Ke Za Zhi ; 55(11): 876-880, 2019 Nov 11.
Artigo em Chinês | MEDLINE | ID: mdl-31715684

RESUMO

Orbital blowout fractures can easily lead to defects of the orbital wall. In order to restore the continuity of the bone wall and avoid a series of clinical symptoms caused by orbital contents herniation or incarceration, the site of the defect should be reconstructed. The effect of reconstruction depends on the choice of surgical plan and repair material. The typical materials for bone wall defect repair include bone sheet, high density porous polyethylene, titanium mesh, absorbable polymer, bioactive ceramics and tissue engineering bone. This paper reviews the research findings and application of material for repairing of orbital blowout fracture. (Chin J Ophthalmol, 2019, 55: 876-880).


Assuntos
Fraturas Orbitárias/cirurgia , Procedimentos Cirúrgicos Reconstrutivos , Materiais Biocompatíveis , Cerâmica , Humanos , Órbita/cirurgia , Polietileno , Polímeros , Telas Cirúrgicas , Engenharia Tecidual , Titânio
11.
J Biomed Nanotechnol ; 15(12): 2332-2350, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31748015

RESUMO

Developing basement membranes (BMs) substitute remains major problem for constructing functional tissue engineered skin because of its complex structure and multifunction of regulating cellular behavior. Herein, a stable electrospinning method was employed to generate a biomimetic model of natural BMs based on novel scaffold electrospun from Poly(ɛ-caprolactone) (PCL) and cellulose acetate (CA) incorporated with chitosan (CS). The morphology, structure, surface hydrophilicity, roughness and mechanical tensile strength of prepared monolayer and tri-layered scaffold were comprehensively compared. Besides, co-culture system via seeding keratinocytes (Kcs) and fibroblasts (Fbs) on opposite side of tri-layered scaffold revealed more effective segregation of both cell types within the central nanofibrous barrier together with enhanced cell attachment and proliferation than that on the monolayer scaffold. Moreover, the deposition of type VII collagen and laminin-5 was examined in comparison with normal skin BMs. Furthermore, the histological studies revealed characteristics of reconstructing BM zone at the junction of dermis-epidermis after in vivo implantation for 2 weeks, and wound healing while the seeded cells interacted with the endogenous cells. Additionally, the expression of active integrin ß1 and phosphorylated focal adhesion kinase (FAK) was promoted with treatment of tri-layered scaffold. This study stressed that this tri-layer scaffold might provoke biomimetic responses of Kcs and Fbs and thus be applied for future development of BMs containing tissues.


Assuntos
Biomimética , Nanofibras , Membrana Basal , Proliferação de Células , Poliésteres , Engenharia Tecidual , Tecidos Suporte
12.
J Biomed Nanotechnol ; 15(12): 2351-2362, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31748016

RESUMO

Hydrogels have been widely used to mimic the biochemical and mechanical environments of native extracellular matrices for cell culture and tissue engineering. Among them, self-assembling peptide hydrogels are of special interest thanks to their great biocompatibility, designability and convenient preparation procedures. In pioneering studies, self-assembling peptide hydrogels have been used for the culture of bone marrow cells. However, the low mechanical stability of peptide hydrogels seems to be a drawback for these applications, as bone marrow cells prefer hard substrates for osteogenic differentiation. In this work, we explored the use of hydroxyapatite (HAP)-peptide hybrid hydrogels for three-dimensional (3D) culture and differentiation of osteogenic MC3T3-E1 cells. We used HAP nanoparticles as crosslinkers to increase the mechanical stability of peptide hydrogels. Meanwhile, HAP provided unique chemical cues to promote the differentiation of osteoblasts. A phosphate group was introduced to the self-assembling peptide so that the peptide fibers could bind to HAP nanoparticles specifically and strongly. Rheological characterization indicated that the hybrid hydrogels were mechanically more stable than the hydrogels containing only peptides and can be used for long term cell culture. Moreover, the hydrogels were biocompatible and showed very low cytotoxicity. The favorable mechanical properties of the hybrid hydrogels and the chemical properties of HAP synergistically supported the differentiation of MC3T3-E1 cells. Based on these characterizations, we believe that these hybrid hydrogels can potentially be used as scaffolds for cartilage and bone regeneration in the future.


Assuntos
Nanopartículas , Osteoblastos , Diferenciação Celular , Durapatita , Hidrogéis , Osteogênese , Peptídeos , Engenharia Tecidual , Tecidos Suporte
13.
J Biomed Nanotechnol ; 15(12): 2363-2375, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31748017

RESUMO

Breast cancer tends to spread to other organs and bone metastasis has the highest frequency in breast cancer metastasis, while its mechanisms are not clear and the current treatments are not very effective. To better study the mechanisms and facilitate drug screening for breast cancer bone metastasis, an in vivo mouse model needs to be constructed. However, the construction of the humanized mouse model for cancer bone metastasis which will mimick real interactions between cancer tissue and bone tissue in the human microenvironment remains a challenge. In this study, we constructed a human engineering bone tissue composed with the human osteoblast-like cells (SaOS-2 cells) and the silica nanoparticlesincorporated human demineralized bone matrix (Si/DBM). The engineered bone was then transplanted into a nude mouse to build a humanized bone microenvironment. The human breast cancer cells were then injected into the fat pads of the nude mouse to form an orthotopic tumor. The results showed that the engineered bone tissue-constructed humanized bone microenvironment had significant advantages when inducing human cancer cells to metastasize into the engineered bone tissue. Further, the SaOS-2/Si/DBM had a stronger ability to entice cancer-bone metastasis through promoting osteogenesis compared to the SaOS-2/DBM. Accordingly, this study highlights a novel, facile and effective mouse model for human cancer-bone metastasis, which will provide a platform to explore the mechanisms and anti-tumor drug screening for cancer-bone metastasis.


Assuntos
Neoplasias Ósseas , Nanopartículas , Animais , Matriz Óssea , Humanos , Camundongos , Dióxido de Silício , Engenharia Tecidual , Microambiente Tumoral
14.
Chem Commun (Camb) ; 55(91): 13631-13637, 2019 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-31680129

RESUMO

The 2019 Faraday Discussion on the Nanolithography of Biointerfaces brought together a diverse set of interdisciplinary scientists involved in the seemingly disparate fields of materials science, nanolithography and glycoscience. The setting and format of this meeting renders the experience unique, and anyone in the audience is instantly engaged in the debate. This Faraday Discussion attracted about sixty delegates, ranging from graduate students and early career researchers to full professors. The meeting was a reflection on how far lithography techniques, tissue engineering and glycoscience have come, with the aid of scientists working at the realm of the nanoscale. True to its name, this gathering was also a discussion on what the outstanding questions in glycobiology are and how nanolithography can be appropriately applied to answer them. In this report, we will give an overview of the topics and discussions covered during the meeting and highlight the content of each session.


Assuntos
Nanotecnologia , Humanos , Engenharia Tecidual
15.
J Photochem Photobiol B ; 201: 111651, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31683166

RESUMO

Designing biomimetic biomaterials influenced by the common complex structure of hard tissues is yet a test these days. The control of bio-mineralization procedure onto biomaterials should be assessed before the use in medical applications. Coming to the bone rejuvenation applications, this work assessed the in vitro antibacterial activity and interacting between osteoblast cells (MG63) on poly (hydroxypropyl methacrylate) (PHPMA) cryogel consolidated with Zn/Ce substituted hydroxyapatite (MHAp) nanocomposite (PHPMA/MHAp). Osteoblast cell multiplication, morphology, and metabolic action were assessed through various conventions. The functional group, texture, mechanical properties, and protein adsorption profiles of the fabricated nanocomposite were analyzed by the FTIR, XRD, SEM, and mechanical examinations, respectively. The bacterial activity of nanocomposites was additionally assessed against E. coli and S. aureus microorganisms, individually. Nanocomposite advanced endo-chondral ossification at the messed up parts of the bone deformity than cryogel did. These results recommend that PHPMA/MHAp nanocomposites joined the good innate properties of each polymer and bioceramic, giving a mechanically powerful, cell-responsive, and permeable stage for hard tissue applications.


Assuntos
Criogéis/química , Durapatita/química , Fraturas do Fêmur/terapia , Nanocompostos/química , Nanopartículas/química , Animais , Regeneração Óssea/efeitos dos fármacos , Adesão Celular/efeitos dos fármacos , Linhagem Celular , Proliferação de Células/efeitos dos fármacos , Cério/química , Modelos Animais de Doenças , Escherichia coli/efeitos dos fármacos , Fraturas do Fêmur/patologia , Nanocompostos/uso terapêutico , Nanocompostos/toxicidade , Polímeros/química , Ratos , Staphylococcus aureus/efeitos dos fármacos , Engenharia Tecidual , Zinco/química
16.
Bratisl Lek Listy ; 120(7): 498-504, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31602984

RESUMO

Using most widespread technology of rapid prototyping (RP) in medicine focus on the development of models for diagnosis, for training and planned surgery, as well as the direct manufacture of implants for bone reconstruction. The applications of 3D printing in the field of medicine are giving extraordinary results and tissue and prosthetic 3D printing, medical and engineering research professionals are conducting 3D printing organ bind. Researchers worldwide are pursuing the creation of artificial bone using 3D printers, bones that can be later implanted to humans. In near future, many body parts could be manufactured in a turn and successfully implanted to patients. Although medical advances in 3D printing are used in orthopaedic field, research in 4D printing has already started. Flat objects made with 3D printing, using a regular plastic, combined with smart material, were able to become a hub without an external intervention. In nutshell, the future of additive manufacturing (AM) in trauma and orthopedic surgery is relatively bright with the inclusion of 3D printing in medicine. Bioprinting in this area will be focused on fractures, nonunions, deformities and bone, cartilage and soft tissue reconstruction. CONCLUSION: The innovative technology not only assists the medical staff but is also beneficial for the patients because the medical problems, which were not curable in the past, are now possible with modern technology (Fig. 4, Ref. 52) Keywords: bone defect, tissue engineering, 3D printing, biomaterials, bone, porous scaffold.


Assuntos
Bioimpressão , Procedimentos Ortopédicos/tendências , Impressão Tridimensional , Materiais Biocompatíveis , Humanos , Engenharia Tecidual
17.
Zhonghua Shao Shang Za Zhi ; 35(9): 641-644, 2019 Sep 20.
Artigo em Chinês | MEDLINE | ID: mdl-31594181

RESUMO

Adipose stem cells (ASCs) are mesenchymal stem cells derived from adipose tissue, and they have potentials of self-renewal and multi-directional differentiation. Compared with bone marrow mesenchymal stem cells, ASCs have many advantages, such as easy access, easy cultivation, and abundant content, which are valuable seed cells in the field of repair and reconstruction. In recent years, with the deepening of the researches on differentiation, regulation, and function of ASCs, the clinical application of ASCs has gradually increased with good therapeutic effects.


Assuntos
Adipócitos/citologia , Terapia Baseada em Transplante de Células e Tecidos , Células-Tronco Mesenquimais/citologia , Engenharia Tecidual , Tecido Adiposo/citologia , Diferenciação Celular , Humanos
18.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi ; 36(5): 795-802, 2019 Oct 25.
Artigo em Chinês | MEDLINE | ID: mdl-31631628

RESUMO

Bone tissue engineering is considered as one of the most promising way to treat large segmental bone defect. When constructing bone tissue engineering graft in vitro, suitable bioreactor is usually used to incubate cell-scaffold complex under perfusion to obtain bone tissue engineering graft with good repair efficiency. However, the theoretical model for growth rate of single cell (especially for stem cell) during this process still has many defects. The difference between stem cells and terminally differentiated cells is always ignored. Based on our previous studies, this study used self-made perfusion apparatus to apply different modes and strengths of fluid shear stress (FSS) to the cells seeded on scaffolds. The effects of FSS on the proliferation and osteogenic differentiation of mesenchymal stem cells (MSCs) were investigated. The regression analysis model of the effect of FSS on the single-cell growth rate of MSCs was further established. The results showed that 0.022 5 Pa oscillatory shear stress had stronger ability to promote proliferation and osteogenic differentiation of MSCs, and the growth rate of a single MSC cell under FSS was modified. This study is expected to provide theoretical guidance for optimizing the perfusion culture condition of bone tissue engineering grafts in vitro.


Assuntos
Células-Tronco Mesenquimais , Diferenciação Celular , Modelos Teóricos , Osteogênese , Engenharia Tecidual , Tecidos Suporte
19.
Adv Exp Med Biol ; 1186: 171-193, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31654390

RESUMO

The success rate from investigational new drug filing to drug approval has remained low for decades despite major scientific and technological advances, and a steady increase of funding and investment. The failure to demonstrate drug efficacy has been the major reason that drug development does not progress beyond phase II and III clinical trials. The combination of two-dimensional (2D) cellular in vitro and animal models has been the gold standard for basic science research and preclinical drug development studies. However, most findings from these systems fail to translate into human trials because these models only partly recapitulate human physiology and pathology. The lack of a dynamic three-dimensional microenvironment in 2D cellular models reduces the physiological relevance, and for these reasons, 3D and microfluidic model systems are now being developed as more native-like biological assay platforms. 3D cellular in vitro systems, microfluidics, self-organized organoids, and 3D biofabrication are the most promising technologies to mimic human physiology because they provide mechanical cues and a 3D microenvironment to the multicellular components. With the advent of human-induced pluripotent stem cell (iPSC) technology, the 3D dynamic in vitro systems further enable extensive access to human-like tissue models. As increasingly complex 3D cellular systems are produced, the use of current visualization technologies is limited due to the thickness and opaqueness of 3D tissues. Tissue-clearing techniques improve light penetration deep into tissues by matching refractive indices among the 3D components. 3D segmentation enables quantitative measurements based on 3D tissue images. Using these state-of-the-art technologies, high-throughput screening (HTS) of thousands of drug compounds in 3D tissue models is slowly becoming a reality. In order to screen thousands of compounds, machine learning will need to be applied to help maximize outcomes from the use of cheminformatics and phenotypic approaches to drug screening. In this chapter, we discuss the current 3D ocular models recapitulating physiology and pathology of the back of the eye and further discuss visualization and quantification techniques that can be implemented for drug screening in ocular diseases.


Assuntos
Avaliação Pré-Clínica de Medicamentos , Oftalmopatias , Modelos Biológicos , Organoides , Engenharia Tecidual , Animais , Avaliação Pré-Clínica de Medicamentos/métodos , Oftalmopatias/patologia , Oftalmopatias/terapia , Humanos , Células-Tronco Pluripotentes Induzidas/citologia , Microfluídica
20.
Zhonghua Shao Shang Za Zhi ; 35(10): 705-711, 2019 Oct 20.
Artigo em Chinês | MEDLINE | ID: mdl-31658539

RESUMO

Artificial dermis is a kind of tissue engineering dermal substitute and is used to repair dermal defects caused by a variety of reasons. This article describes the characteristics and the mechanism of repair and reconstruction of bilayer artificial dermis. Based on domestic experience of clinical applications and relative literature of bilayer artificial dermis, more than 50 domestic experts in related field reached a consensus on indications, contraindications, operation procedures in clinical application, cautions, and treatment and prevention of complications of bilayer artificial dermis, providing reference for clinical application.


Assuntos
Derme/patologia , Transplante de Pele/métodos , Pele Artificial , Engenharia Tecidual , Consenso
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