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1.
J Cosmet Sci ; 71(2): 53-64, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32271708

RESUMO

The aim of the study was to investigate the potential of a fucoxanthin concentrate prepared from Phaeodactylum tricornutum as a wrinkle care cosmetic agent. The concentrate (up to 25 µg/ml) did not affect the proliferation of human fibroblasts. In addition, the concentrate significantly increased procollagen synthesis in the fibroblasts at 12.5 and 25 µg/ml; however, it significantly decreased the expression of matrix metalloproteinase (MMP)-1, MMP-2, and MMP-9 at 25 µg/ml. In a follow-up study, a wrinkle care cream containing 0.03% of fucoxanthin concentrate was prepared and tested in women (aged 35-50 years, n = 21) for 8 weeks. The cream was applied twice daily. Safety assessment of the cream was carried out visually. In addition, interviews were conducted to investigate if adverse events such as erythema, edema, scaling, itching, stinging, burning, tightness, or prickling had occurred. No symptoms that threaten skin safety were reported. Evaluation of wrinkles around the eyes using the replica method showed a statistically significant decrease in wrinkles at week 8. Moreover, skin moisture and elasticity increased significantly from week 4. These results suggest that the fucoxanthin concentrate has no adverse effects on the skin and can be used as an active ingredient in wrinkle care cosmetics.


Assuntos
Cosméticos , Envelhecimento da Pele , Xantofilas/farmacologia , Adulto , Feminino , Seguimentos , Humanos , Pessoa de Meia-Idade
2.
Medicine (Baltimore) ; 99(13): e19381, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32221066

RESUMO

Thread lift boasts the advantage of minimal invasion for facial rejuvenation and has been increasingly used nowadays. The purpose of this study was to evaluate the outcomes and safety of elastic thread when it was used in the modified minimal access cranial suspension (MACS) lift for rejuvenation of the lower and middle third of the face.Forty-six patients with sagging and laxity of the lower face treated by the elastic thread modified MACS lift from December 2015 and December 2017 were enrolled in this study. A retrospective chart review was conducted. The degree of Wrinkle Severity Rating Scale (WSRS) score and satisfaction score were evaluated immediately, 6 months and 12 months after procedure. Complications during the study were also recorded.All the patients were female, with a mean age of 50.7 ±â€Š6.4 years and a mean follow-up period of 15.4 ±â€Š2.1 months. The mean operation time was 114 ±â€Š13 minutes. For the left face, the mean WSRS score was 4.0 ±â€Š0.8 preoperatively and 3.1 ±â€Š0.8 on the 1-year follow-up; and 4.1 ±â€Š0.9 and 3.1 ±â€Š0.7 on the right face (P < .01). Thirty-nine (84.8%) patients considered the long-term results satisfactory. There were no major complications during the follow-up period.The elastic thread modified MACS lift is a minimally invasive, effective and safe method to improve lagging middle and lower third of the face without significant postoperative morbidity or complications.


Assuntos
Face , Ritidoplastia/métodos , Técnicas de Sutura , Adulto , Elasticidade , Feminino , Humanos , Pessoa de Meia-Idade , Procedimentos Cirúrgicos Minimamente Invasivos , Duração da Cirurgia , Satisfação do Paciente , Rejuvenescimento , Envelhecimento da Pele
3.
Plast Reconstr Surg ; 145(2): 345-353, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31985619

RESUMO

BACKGROUND: Horizontal neck wrinkles develop as a result of the aging process. Stromal vascular fraction (SVF) gel, which is rich in extracellular matrix and functional cells, can be produced by a series of simple mechanical processes, including intersyringe shifting and centrifugation. This study aimed to assess stromal vascular fraction gel in the treatment of horizontal neck wrinkles. METHODS: This single-center study included female patients with horizontal neck wrinkles (Fitzpatrick types II to IV) treated with either SVF gel or botulinum toxin type A (BTX A) injection. SVF gel was first diffusely distributed subcutaneously along the neck line and then injected in a diluted way intracutaneously at points 0.5 cm apart along the horizontal lines. BTX A was injected at points 1.5 cm apart (2 U in each injection site). Satisfaction and improvement scores were compared between the two groups, and the collagen content of the neck wrinkle was compared by histologic evaluation before and after treatment. RESULTS: Twenty-eight patients received SVF gel and 22 received BTX A. In patients with type II neck wrinkles, BTX A and SVF gel treatment resulted in similar improvement scores and patient satisfaction in the first 3 months. In patients with type III and IV neck wrinkles, SVF gel resulted in significantly higher improvement scores and better patient satisfaction. A longer duration of adverse events was seen in the SVF gel treatment group. Histologic assessment suggested that SVF gel increased the collagen density of neck wrinkles. CONCLUSION: SVF gel is an effective treatment for horizontal neck wrinkles, particularly in patients with type III and IV wrinkles. CLINICAL QUESTION/LEVEL OF EVIDENCE: Therapeutic, II.


Assuntos
Toxinas Botulínicas Tipo A/administração & dosagem , Matriz Extracelular , Células-Tronco Mesenquimais , Fármacos Neuromusculares/administração & dosagem , Envelhecimento da Pele , Adulto , Terapia Baseada em Transplante de Células e Tecidos/métodos , Feminino , Géis , Humanos , Injeções Subcutâneas , Pescoço , Satisfação do Paciente , Resultado do Tratamento
4.
Plast Reconstr Surg ; 145(2): 306e-315e, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31985625

RESUMO

BACKGROUND: Patients with horizontal lower lid laxity undergoing lower lid blepharoplasty are at a high risk for lid malposition. Prophylactic correction of horizontal lower lid laxity during blepharoplasty may alleviate this risk. In this article, the authors describe a novel lower lid blepharoplasty technique, the KS-pexy (named after its originators, Kuhnt and Szymanowski), for correcting horizontal lower lid laxity. METHODS: All cases of KS-pexy between September of 2009 and June of 2018 from an aesthetic practice were reviewed retrospectively. All patients were diagnosed with horizontal lower lid laxity and requested lower lid blepharoplasty for aesthetic concerns. Patient demographics, clinical presentation, procedure details, and intraoperative findings were analyzed. RESULTS: Sixty-two patients underwent KS-pexy performed by the senior author. The average follow-up was 41.1 months, ranging from 2 to 107 months. Thirteen patients had a negative vector, 54 patients received a KS-pexy in combination with another facial procedure, and 14 patients had their KS-pexy as a secondary procedure. The overall success rate was 96.8 percent. The complication profile was as follows: chemosis (27.4 percent), new-onset dry eyes persisting longer than 8 weeks (6.5 percent), temporary lower eyelid malposition (4.8 percent), lower eyelid malposition requiring surgical intervention (3.2 percent), poor scar quality (1.6 percent), lower lid abscess (1.6 percent), transient conjunctivitis (1.6 percent), lateral fat compartment swelling (1.6 percent), canthopexy dehiscence (1.6 percent), hordeolum (1.6 percent), and temporary V2 paresthesia (1.6 percent). CONCLUSION: The KS-pexy is an effective means to treat horizontal eyelid laxity prophylactically in patients requesting lower eyelid blepharoplasty for aesthetic reasons. CLINICAL QUESTION/LEVEL OF EVIDENCE: Therapeutic, IV.


Assuntos
Blefaroplastia/métodos , Adulto , Idoso , Pálpebras/cirurgia , Feminino , Seguimentos , Humanos , Masculino , Pessoa de Meia-Idade , Envelhecimento da Pele
5.
Chem Biol Interact ; 315: 108910, 2020 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-31790661

RESUMO

Ursonic acid is a pentacyclic triterpenoid compound that can be extracted from Ziziphus jujuba Mill., a traditional medicine. Matrix metalloproteinases (MMPs) are involved in cancer metastasis and skin aging. Regulation of various MMPs is closely associated with mitogen-activated protein kinases (MAPKs), including ERK, p38, and JNK MAPKs. In this study, we investigated the possibility of ursonic acid as an anti-cancer/anti-skin aging agent targeting MMPs. Cytotoxic effects of ursonic acid were analyzed by cell counting kit-8 (CCK-8) assay. Invasive abilities of ursonic acid-treated A549 and H1299 non-small cell lung cancer (NSCLC) cells were tested with Boyden chamber assay. Effects of ursonic acid on MMPs were analyzed by zymography assays and quantitative real time polymerase chain reaction (qRT-PCR). We also conducted flow cytometry and western blot analysis to elucidate the mechanisms of MMP regulation by ursonic acid. Our results revealed that ursonic acid inhibited transcriptional expression of gelatinases (MMP-2 and MMP-9) via inhibition of ERK and CREB signaling pathways in NSCLC cells. Moreover, ursonic acid reduced mRNA levels of collagenase (MMP-1) via suppression of ERK and c-Fos signaling pathways in HaCaT keratinocytes. These results suggest that ursonic acid could be a potential candidate for development of an effective novel anti-cancer and anti-wrinkle agent.


Assuntos
Sistema de Sinalização das MAP Quinases/efeitos dos fármacos , Metaloproteinases da Matriz/metabolismo , Transdução de Sinais/efeitos dos fármacos , Triterpenos/farmacologia , Células A549 , Antineoplásicos/farmacologia , Carcinoma Pulmonar de Células não Pequenas/tratamento farmacológico , Carcinoma Pulmonar de Células não Pequenas/metabolismo , Linhagem Celular Tumoral , Humanos , Queratinócitos/efeitos dos fármacos , Queratinócitos/metabolismo , Neoplasias Pulmonares/tratamento farmacológico , Neoplasias Pulmonares/metabolismo , Envelhecimento da Pele/efeitos dos fármacos , Transcrição Genética/efeitos dos fármacos
6.
Clin Plast Surg ; 47(1): 31-41, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31739895

RESUMO

Injectable tissue replacement and regeneration (ITR2) is a standardized fat grafting technique, which anatomically addresses losses of facial volume, laxity, and sun damage of the skin resulting from the natural processes of aging. Based on the structural differences of fat existing in the deeper versus the superficial fat compartments of the face, while accounting for skeletal losses and skin aging, ITR2 utilizes 3 sizes of fat grafts-millifat (parcel size 2.0-2.5 mm), microfat (1.0 mm), and nanofat (<500 µm)-to replicate characteristics of fat cells lost with facial decay and provide increased blood supply and improvements in aging skin.


Assuntos
Tecido Adiposo/transplante , Face/cirurgia , Procedimentos Cirúrgicos Reconstrutivos , Adipócitos , Humanos , Regeneração , Rejuvenescimento , Envelhecimento da Pele
7.
Clin Plast Surg ; 47(1): 43-51, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31739896

RESUMO

Fat grafting is one of the most prevalent fields in facial rejuvenation at present. The procedures include the evaluation of recipient and donor areas, as well as fat harvesting, processing, and reinjection. Inappropriate treatment of each link may affect the final effect on the whole. The authors believe that understanding of facial anatomy and personalized therapy are the key to successful operation.


Assuntos
Tecido Adiposo/transplante , Grupo com Ancestrais do Continente Asiático , Face/cirurgia , Procedimentos Cirúrgicos Reconstrutivos , Humanos , Rejuvenescimento , Envelhecimento da Pele
8.
Clin Plast Surg ; 47(1): 53-62, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31739897

RESUMO

Reversing structural changes in aging skin gained potential after the therapeutic use of adipose-derived stem cells was described. Nanofat is a highly concentrated solution of progenitor cells without viable adipocytes. Nanofat grafting creates striking skin quality improvement. The availability of adipose-tissue combined with straightforward mechanical protocol to process fat brings regenerative and antiaging medicine into real-life clinical practice. Association with other cofactors (hyaluronic acid, botulin toxin, and vitamin C) and therapies (microneedling and drug delivery) provides better outcomes. This article describes the techniques and the authors' experiences in nanofat grafting, and its potential new applications in regenerative medicine.


Assuntos
Tecido Adiposo/transplante , Face/cirurgia , Procedimentos Cirúrgicos Reconstrutivos , Medicina Regenerativa/métodos , Transplante de Células-Tronco , Adipócitos/transplante , Humanos , Rejuvenescimento , Envelhecimento da Pele , Células-Tronco
9.
J Sci Food Agric ; 100(2): 672-681, 2020 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-31583701

RESUMO

BACKGROUND: Hibiscus sabdariffa is commonly used in daily life and its extract is applied widely in food and cosmetics. However, it has not been evaluated for its anti-aging effects. RESULTS: Hibiscus sabdariffa calyx aqueous extract (HSCAE) has shown potential collagenase activity suppression effects, together with tyrosinase activity inhibition, and anti-oxidation as a free radical scavenger. The current investigation demonstrated that HSCAE was not cytotoxic in skin fibroblasts, and it significantly decreased ultraviolet B (UVB)-induced reactive oxygen species (ROS) on a flow cytometry assay. Moreover, HSCAE reduced matrix metalloproteinase (MMP) expression, increased tissue inhibition of metalloproteinase (TIMP)-1 level, and enhanced collagen content by inhibiting collagenase activity. It also blocked mRNA and protein expressions of melanin production pathway key factors, including the microphthalmia-associated transcription factor (MITF), tyrosinase, tyrosinase-related protein-1 (TRP-1), and dopachrome tautomerase-2 (TRP-2). CONCLUSION: These results demonstrated, for the first time, the potential of HSCAE as a natural antioxidant with the ability to maintain collagen production and to decrease melanin syntheses under UVB radiation, for anti-aging effects. © 2019 Society of Chemical Industry.


Assuntos
Depuradores de Radicais Livres/farmacologia , Hibiscus/química , Extratos Vegetais/farmacologia , Envelhecimento da Pele/efeitos dos fármacos , Animais , Linhagem Celular , Fibroblastos/efeitos dos fármacos , Fibroblastos/metabolismo , Fibroblastos/efeitos da radiação , Humanos , Oxirredutases Intramoleculares/genética , Oxirredutases Intramoleculares/metabolismo , Camundongos , Monofenol Mono-Oxigenase/genética , Monofenol Mono-Oxigenase/metabolismo , Oxirredutases/genética , Oxirredutases/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Envelhecimento da Pele/efeitos da radiação , Raios Ultravioleta/efeitos adversos
10.
Plast Reconstr Surg ; 145(1): 58e-66e, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-31881607

RESUMO

The practice of chemical peeling remains an important aspect of the nonsurgical techniques available to the aesthetic surgeon when approaching skin rejuvenation. Despite past predictions of their disappearance in favor of lasers, the overall use of chemical peels by plastic surgeons continues to grow. In the past two decades, the techniques available to the clinician have evolved in safety and efficacy based on the dermatologic investigations of various individuals, including Obagi, Hetter, and Stone. The versatility, clinical endpoint-directed predictability, and favorable risk profile of chemical peels proffered by these latest advancements affirm that this modality is essential to the practice of the plastic surgeon treating patients with rhytides and dyschromias. This review presents the current role of chemical peels in skin rejuvenation, emphasizing the significant clinical advancements and their modern day applications and practice.


Assuntos
Cáusticos/administração & dosagem , Abrasão Química/tendências , Técnicas Cosméticas/tendências , Rejuvenescimento , Abrasão Química/efeitos adversos , Abrasão Química/métodos , Técnicas Cosméticas/efeitos adversos , Estética , Face , Humanos , Envelhecimento da Pele , Resultado do Tratamento
11.
Dermatol Surg ; 46(1): 50-60, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-30829771

RESUMO

BACKGROUND: Patient-reported outcomes are increasingly recognized as important measures of treatment benefit. OBJECTIVE: To evaluate subject-reported satisfaction and impact outcomes with onabotulinumtoxinA treatment in neurotoxin-naive adults with forehead lines (FHL), glabellar lines (GL), and crow's feet lines (CFL). METHODS: This Phase 3 study randomized 787 subjects to onabotulinumtoxinA 64 U (FHL 20 U, GL 20 U, and CFL 24 U), 40 U (FHL 20 U, GL 20 U, and CFL placebo), or placebo in double-blind Period 1. Subjects could receive up to 2 additional 64 U treatments in open-label Period 2. Patient-reported outcomes were assessed using the validated Facial Line Satisfaction Questionnaire (FLSQ) and 11-item Facial Line Outcomes (FLO-11) Questionnaire. RESULTS: The proportion of subjects mostly or very satisfied was significantly greater with onabotulinumtoxinA 64 U and 40 U versus placebo (87.9% and 81.4% vs 3.2%; p < .0001). Responder rates on FLSQ Impact Domain, FLO-11 Items 1, 4, 5, and total score were significantly greater with onabotulinumtoxinA versus placebo on Day 30 (p < .0001). Responder rates favoring onabotulinumtoxinA in Period 1 were maintained with repeated onabotulinumtoxinA 64 U treatment in Period 2. CONCLUSION: OnabotulinumtoxinA treatment was associated with high subject satisfaction and significant improvements in appearance-related psychological and emotional impacts.


Assuntos
Toxinas Botulínicas Tipo A/uso terapêutico , Técnicas Cosméticas , Testa , Fármacos Neuromusculares/uso terapêutico , Envelhecimento da Pele , Adulto , Idoso , Método Duplo-Cego , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Medidas de Resultados Relatados pelo Paciente , Satisfação do Paciente , Adulto Jovem
12.
Dermatol Surg ; 46(1): 41-49, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-30883477

RESUMO

BACKGROUND: Hyaluronic acid (HA) fillers are the most commonly used fillers for soft-tissue augmentation. The face is a dynamic structure. Facial rejuvenation by filler products depends on mechanical forces on the region of the face. The successful use of injectable HA fillers requires an understanding of the options available. OBJECTIVE: The purpose of this study is to measure the rheological properties of HA fillers and to clarify how to select these fillers considering their rheological properties. MATERIALS AND METHODS: Rheological characterization was performed on 41 fillers. Physical parameters directly linked to product performance were measured. RESULTS: The properties of the HA fillers varied. These findings provide a basis for selection guideline regarding rheological properties in facial rejuvenation. CONCLUSION: The authors' report is the largest study to determine the rheological properties of HA fillers to date. Understanding the fillers' properties can help physicians select the appropriate fillers for more predictable and sustainable results.


Assuntos
Técnicas Cosméticas , Preenchedores Dérmicos/farmacologia , Face , Ácido Hialurônico/farmacologia , Rejuvenescimento , Viscossuplementos/farmacologia , Humanos , Guias de Prática Clínica como Assunto , Reologia , Envelhecimento da Pele
13.
Dermatol Surg ; 46(1): 61-69, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-31356432

RESUMO

BACKGROUND: The aesthetic use of botulinum toxins is widely accepted in routine treatment of glabellar lines. OBJECTIVE: To strengthen existing efficacy and safety data, and examine subject satisfaction concerning abobotulinumtoxinA (ABO) treatment for moderate to severe glabellar lines. MATERIALS AND METHODS: A 150-day prospective, single-dose, multicenter, randomized, parallel-group, placebo-controlled, double-blind study of 300 subjects treated with ABO (50 Units [U]). Response was defined as having no or mild glabellar lines after treatment (primary objective was Day 30 response). Glabellar line severity, onset and duration of effect, satisfaction, treatment emergent adverse events (TEAEs), and treatment-related adverse events (TRAEs) were assessed. RESULTS: Median time to onset of effect was 2 days. Day 30, ABO treatment response was 89% and 85% for investigator and subject assessments, respectively (p < .001 vs placebo). At Day 120, 60% of subjects demonstrated ≥1 grade improvement and 45% at Day 150. Subject satisfaction concerning glabellar line appearance was high (93%) at Day 14 and maintained by 35% through Day 150 with ABO treatment. No serious AEs were related to study treatment. CONCLUSION: Most ABO-treated subjects sustained ≥1 grade glabellar line improvements over 120 days, and satisfaction was maintained by >30% of subjects throughout 150 days. ABO was well tolerated.


Assuntos
Toxinas Botulínicas Tipo A/uso terapêutico , Técnicas Cosméticas , Testa , Fármacos Neuromusculares/uso terapêutico , Envelhecimento da Pele , Adulto , Idoso , Método Duplo-Cego , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Satisfação do Paciente , Fatores de Tempo , Resultado do Tratamento , Adulto Jovem
14.
Eur J Pharm Sci ; 141: 105042, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31634554

RESUMO

Skin aging affects personal image and health. α - lipoic acid (ALA), with excellent free radical scavenging capacity, was used in this research to prepare W/O emulsion. Considering the instability of ALA, ionic liquid strategy was adopted to heighten the solubility of ALA for dissolving in water phase. The mechanism of different ionic liquids (ILs) on skin retention of ALA was investigated by in vitro skin permeation experiment, emulsion quality characterization, rheological test, ATR - FTIR and molecular simulation. The results showed that ionic liquid strategy had a positive influence on the solubilization of ALA. Different ILs were different in skin retention and regulated by skin layers rather than drug release, in which ALA - triethanolamine (ALA - TEOA) presented the best affinity with both stratum corneum (SC) and viable epidermis and dermis (VED), while ALA - N - (2 - Hydroxyethyl) piperidine (ALA - HEPP) as well as ALA - N - (2 - hydroxyethyl) pyrrolidine (ALA - HEPR) showed affinity with either SC or VED respectively. Finally, the emulsion presented brilliant anti - aging efficacy. This study provided a new method of emulsion research and had great significance for the development of topical formulations.


Assuntos
Líquidos Iônicos/administração & dosagem , Envelhecimento da Pele/efeitos dos fármacos , Ácido Tióctico/administração & dosagem , Administração Cutânea , Animais , Emulsões , Hidroxiprolina/metabolismo , Líquidos Iônicos/química , Masculino , Ratos Wistar , Pele/efeitos dos fármacos , Pele/metabolismo , Pele/efeitos da radiação , Absorção Cutânea/efeitos dos fármacos , Envelhecimento da Pele/efeitos da radiação , Ácido Tióctico/química , Raios Ultravioleta/efeitos adversos
15.
Yakugaku Zasshi ; 139(12): 1553-1556, 2019.
Artigo em Japonês | MEDLINE | ID: mdl-31787644

RESUMO

The skin is the largest human organ, comprising the epidermis that is composed of epithelial tissue, the dermis composed of connective tissue, and the innermost subcutaneous tissue. Generally, skin conditions are due to aging and the influence of the external environment, but empirically patients with gastrointestinal diseases are more prone to pruritus and inflammation caused by dry skin. A decrease in the skin barrier function, involving immunocompetent mast cells and oxidative stress, was noted in indomethacin-induced small intestine inflammation, dextran sodium sulfate (DSS)-induced ulcerative colitis, and azoxymethane+DSS-induced colorectal cancer. A possible correlation was found to exist between inflammatory gastrointestinal diseases and the skin, and this correlation was investigated using a rheumatoid arthritis model as representative of inflammatory diseases. Similar to previously reported results, deterioration of the skin barrier function was observed, and new information was obtained by analyzing changes in inflammatory markers in the blood and skin tissues. Understanding the underlying mechanism of decreased skin barrier function will help in establishing effective prophylaxis and treatment methods and clarify the importance of crosstalk between organs. It will also help accelerate drug development.


Assuntos
Artrite Reumatoide/complicações , Gastroenterite/complicações , Dermatopatias/etiologia , Fenômenos Fisiológicos da Pele , Pele/fisiopatologia , Animais , Modelos Animais de Doenças , Humanos , Camundongos , Envelhecimento da Pele
16.
Adv Gerontol ; 32(4): 509-515, 2019.
Artigo em Russo | MEDLINE | ID: mdl-31800177

RESUMO

The aim of this work was to examine the content of Yes-associated protein (YAP) in fibroblasts and blood microvessels of human dermis from the development until deep aging (from 20 weeks of pregnancy until 85 years old), and defining of a role of YAP in age-dependent changes in the number of fibroblasts and blood microvessels in the dermis. YAP, proliferating cells nuclear antigen (PCNA), endothelial cells marker CD31 were detected with indirect immunohistochemical technique. Results showed that portion of fibroblasts with positive staining for YAP in the dermis is decreased from 20 weeks of pregnancy to 40 years old. Percent of YAP positive fibroblasts in dermis is increased sufficiently since 41 years old until 60-85 years old group. The content of YAP in blood microvessels in the human dermis is decreased sufficiently from 20 weeks of pregnancy until 40 years old. Age-related changes in the content of YAP in fibroblasts and blood microvessels is not statistically associated with an age-related decrease in total number and percent of PCNA positive fibroblasts, the number of blood vessels in the dermis.


Assuntos
Proteínas Adaptadoras de Transdução de Sinal , Células Endoteliais , Envelhecimento da Pele , Fatores de Transcrição , Proteínas Adaptadoras de Transdução de Sinal/genética , Proteínas Adaptadoras de Transdução de Sinal/metabolismo , Adulto , Idoso , Idoso de 80 Anos ou mais , Envelhecimento , Derme/citologia , Derme/metabolismo , Feminino , Fibroblastos/metabolismo , Humanos , Pessoa de Meia-Idade , Gravidez , Envelhecimento da Pele/fisiologia , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo
17.
An Acad Bras Cienc ; 91(4): e20181088, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31800700

RESUMO

Aged skin, featured with dryness and wrinkles, has received mounting attention due to its adverse influences on beauty. ß-Sitosterol and vermicularin are two common active ingredients of Thamnolia vermicularis (Sw.) Ach., a traditional Chinese medicine, of which the anti-aging effect has been discovered. Their protective performance against skin aging was assayed by co-culturing with skin cells in this work. Results showed that ß-sitosterol promoted the biosynthesis of hyaluronic acid by increasing the expression of hyaluronic acid synthases in fibroblasts and enhanced the expression of skin barrier functional proteins including aquaporin 3, loricrin, filaggrin and involucrin in keratinocytes, which conduced to the moisture retention within skin. Moreover, vermicularin might function as an anti-wrinkle agent by preventing the loss of collagen type I. Specifically, vermicularin reduced the amount of reactive oxygen species within hydrogen-peroxide-induced fibroblasts; together with suppressing the activation of mitogen-activated protein kinases, it could inhibit the production of matrix metalloproteinases-1. The present research will contribute to the development of the compounds as anti-aging ingredients for future applications in cosmetic formulations and functional food as well as promote further studies of raw materials containing alike compounds.


Assuntos
Fibroblastos/efeitos dos fármacos , Queratinócitos/efeitos dos fármacos , Extratos Vegetais/farmacologia , Sitosteroides/farmacologia , Envelhecimento da Pele/efeitos dos fármacos , Linhagem Celular , Humanos , Substâncias Protetoras/farmacologia
18.
J Cosmet Sci ; 70(6): 277-290, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31829921

RESUMO

Previous studies have shown that the date palm kernel contains plenty of phytochemicals of potential rejuvenation benefits to skin. The aim of this study was to investigate a cream form containing date palm kernel extract (DPKE) on facial wrinkle reduction and objective skin parameters in healthy subjects. A cream form containing 5% DPKE was prepared and applied twice daily for 8 weeks on the facial skin of 43 volunteers. Biophysical measurements including skin hydration, elasticity, and pigmentation as well as optical scanning of skin surface were carried out after 4 and 8 weeks. Significant improvement in facial skin hydration, elasticity, and melanin concentration together with reduction in the wrinkle size and depth were observed at the two time points of measurements. In addition, DPKE cream was extremely well tolerated by the facial skin of study participants. The work herein demonstrates and validates the use of cream form containing 5% DPKE over placebo against fine lines and wrinkles, skin pigmentations, skin hydration, and elasticity. This effect may be attributed to synergism of major phytochemicals and phytosterols present in DPKE.


Assuntos
Phoeniceae , Envelhecimento da Pele , Face , Humanos , Extratos Vegetais , Pele
19.
Zhongguo Zhong Yao Za Zhi ; 44(21): 4677-4684, 2019 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-31872664

RESUMO

Chinese herbal medicine ultrafine powder has become a research hotspot for the addition of cosmetic raw materials. Dendrobium candidum is a traditional Chinese herbal medicine. Its extract and stem extract are already cosmetic raw materials and its water extract has the effect of preventing photoaging,but D. candidum ultrafine powder has not been accepted as a raw material for cosmetics,and no relevant research on photoaging prevention has been reported. In this experiment,the ultra-fine powder and fine powder of D. candidum to prevent photoaging were observed and compared,and its mechanism of action was discussed to provide a basis for the prevention of skin photoaging products. Seventy-two female ICR mice were randomly divided into normal group,model group,solvent group,titanium dioxide(Ti O2) group,isooctyl salicylate(2-ES) group,D. candidum ultrafine powder 1(DP1),ultrafine powder 2(DP2) and fine powder(DP3) groups. The photoaging model was established by ultraviolet irradiation for 8 weeks,and the model was intervened while modeling. The skin wrinkle grade,elastic parameters,skin microcirculation blood flow,skin structure and pathological changes(skin thickness,skin collagen fiber,elastic fiber) were observed,the skin transforming growth factor-ß1(TGF-ß1),Smad3 levels were determined,and the type Ⅰ and type Ⅲ collagen,matrix metalloproteinase-1(MMP-1),activated protein-1(AP-1),VEGF expression were detected. The results showed that ultrafine powder(DP1,DP2) significantly reduced the wrinkle level and skin blood flow of the model mice(P<0. 05,P<0. 01); DP1,DP2 and DP3 could significantly reduce the thickness of the epidermis(P<0. 001),improve collagen fiber,elastic fiber hyperplasia,and distortion and decrease VEGF expression,and DP1 is better than DP2 and DP3; each group could up-regulate type Ⅰ collagen,down-regulate type Ⅲ collagen,AP-1,MMP-1 protein expression,and DP1 improvement optimal. However,it has no obvious effect on TGF-ß1 and Smad3. The ultrafine powder and fine powder of D. candidum have certain preventive effect on photoaging,and the effect of ultrafine powder is better than that of fine powder. Ultrafine powder may down-regulate the expression of type Ⅲ collagen,AP-1 and MMP-1 by up-regulating type Ⅰ collagen. Inhibition of collagen degradation plays a role in preventing photoaging.


Assuntos
Dendrobium , Envelhecimento da Pele , Animais , Feminino , Camundongos , Camundongos Pelados , Camundongos Endogâmicos ICR , Pele , Raios Ultravioleta
20.
Int J Mol Sci ; 21(1)2019 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-31861912

RESUMO

The dermal-epidermal junction (DEJ) provides a physical and biological interface between the epidermis and the dermis. In addition to providing a structural integrity, the DEJ also acts as a passageway for molecular transport. Based on the recently reported importance of the DEJ in skin aging, novel peptide derivatives have been tested for their effects on basement membrane (BM) protein expressions in cultured human epidermal keratinocytes. As a result, protein expressions of collagen XVII, laminin and nidogen were stimulated by the test peptide and peptides complex. Further ex vivo evaluation using excised human skin, confirmed that the topical application of the peptides complex significantly increased dermal collagen expression, as well as expressions of collagen XVII and laminin. Interestingly, while the origin of the laminin protein is epidermal keratinocytes, the immunohistochemical staining of skin showed that laminin was only detected in the uppermost layer of the dermis, which suggests a tight assembly of laminin protein onto the dermal side of the DEJ. These results suggest that a peptide complex could improve the structural properties of the DEJ through its ability to stimulate BM proteins. In order to evaluate the anti-wrinkle benefits of the peptide complex in vivo, a clinical study was performed on 22 healthy Asian female volunteers older than 40 years. As a result, significant improvements in skin wrinkles for all of the five sites were observed after two weeks, as assessed by skin topographic measurements. Collectively, these results demonstrate the anti-aging efficacy of the peptides complex.


Assuntos
Membrana Basal/efeitos dos fármacos , Queratinócitos/efeitos dos fármacos , Peptídeos/farmacologia , Envelhecimento da Pele/efeitos dos fármacos , Pele/efeitos dos fármacos , Adulto , Autoantígenos/análise , Linhagem Celular , Colágeno Tipo I/análise , Feminino , Humanos , Queratinócitos/química , Queratinócitos/citologia , Laminina/análise , Pessoa de Meia-Idade , Colágenos não Fibrilares/análise , Pele/química , Pele/citologia
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