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1.
J Sci Food Agric ; 100(2): 672-681, 2020 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-31583701

RESUMO

BACKGROUND: Hibiscus sabdariffa is commonly used in daily life and its extract is applied widely in food and cosmetics. However, it has not been evaluated for its anti-aging effects. RESULTS: Hibiscus sabdariffa calyx aqueous extract (HSCAE) has shown potential collagenase activity suppression effects, together with tyrosinase activity inhibition, and anti-oxidation as a free radical scavenger. The current investigation demonstrated that HSCAE was not cytotoxic in skin fibroblasts, and it significantly decreased ultraviolet B (UVB)-induced reactive oxygen species (ROS) on a flow cytometry assay. Moreover, HSCAE reduced matrix metalloproteinase (MMP) expression, increased tissue inhibition of metalloproteinase (TIMP)-1 level, and enhanced collagen content by inhibiting collagenase activity. It also blocked mRNA and protein expressions of melanin production pathway key factors, including the microphthalmia-associated transcription factor (MITF), tyrosinase, tyrosinase-related protein-1 (TRP-1), and dopachrome tautomerase-2 (TRP-2). CONCLUSION: These results demonstrated, for the first time, the potential of HSCAE as a natural antioxidant with the ability to maintain collagen production and to decrease melanin syntheses under UVB radiation, for anti-aging effects. © 2019 Society of Chemical Industry.


Assuntos
Depuradores de Radicais Livres/farmacologia , Hibiscus/química , Extratos Vegetais/farmacologia , Envelhecimento da Pele/efeitos dos fármacos , Animais , Linhagem Celular , Fibroblastos/efeitos dos fármacos , Fibroblastos/metabolismo , Fibroblastos/efeitos da radiação , Humanos , Oxirredutases Intramoleculares/genética , Oxirredutases Intramoleculares/metabolismo , Camundongos , Monofenol Mono-Oxigenase/genética , Monofenol Mono-Oxigenase/metabolismo , Oxirredutases/genética , Oxirredutases/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Envelhecimento da Pele/efeitos da radiação , Raios Ultravioleta/efeitos adversos
2.
Eur J Pharm Sci ; 141: 105042, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31634554

RESUMO

Skin aging affects personal image and health. α - lipoic acid (ALA), with excellent free radical scavenging capacity, was used in this research to prepare W/O emulsion. Considering the instability of ALA, ionic liquid strategy was adopted to heighten the solubility of ALA for dissolving in water phase. The mechanism of different ionic liquids (ILs) on skin retention of ALA was investigated by in vitro skin permeation experiment, emulsion quality characterization, rheological test, ATR - FTIR and molecular simulation. The results showed that ionic liquid strategy had a positive influence on the solubilization of ALA. Different ILs were different in skin retention and regulated by skin layers rather than drug release, in which ALA - triethanolamine (ALA - TEOA) presented the best affinity with both stratum corneum (SC) and viable epidermis and dermis (VED), while ALA - N - (2 - Hydroxyethyl) piperidine (ALA - HEPP) as well as ALA - N - (2 - hydroxyethyl) pyrrolidine (ALA - HEPR) showed affinity with either SC or VED respectively. Finally, the emulsion presented brilliant anti - aging efficacy. This study provided a new method of emulsion research and had great significance for the development of topical formulations.


Assuntos
Líquidos Iônicos/administração & dosagem , Envelhecimento da Pele/efeitos dos fármacos , Ácido Tióctico/administração & dosagem , Administração Cutânea , Animais , Emulsões , Hidroxiprolina/metabolismo , Líquidos Iônicos/química , Masculino , Ratos Wistar , Pele/efeitos dos fármacos , Pele/metabolismo , Pele/efeitos da radiação , Absorção Cutânea/efeitos dos fármacos , Envelhecimento da Pele/efeitos da radiação , Ácido Tióctico/química , Raios Ultravioleta/efeitos adversos
3.
BMC Complement Altern Med ; 19(1): 286, 2019 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-31660950

RESUMO

BACKGROUND: Edible insects, including Oxya chinensis sinuosa Mishchenko (Oc), which is consumed as food in Asia, are considered as a human food shortage alternative, and also as a preventive measure against environmental destruction. Ultraviolet B (UVB) irradiation, which causes skin photodamage, is considered as an extrinsic skin aging factor. It reduces skin hydration, and increases wrinkle formation and reactive oxygen species (ROS) and inflammatory cytokine expression. Thus, the objective of this study was to investigate the anti-aging effects of an ethanol extract of Oc (Oc.Ex). METHODS: A UVB-irradiated hairless mouse model was used to examine relevant changes in skin hydration, wrinkle formation, and skin epidermal thickness. Also, antioxidant markers such as superoxide dismutase (SOD) and catalase (CAT) were analyzed, and Oc. Ex skin protective effects against UVB irradiation-induced photoaging were examined by determining the levels of skin hydration factors. RESULTS: Oc.Ex improved epidermal barrier dysfunctions such as increased transepidermal water loss (TEWL) and capacitance reduction in UVB-irradiated mice. It upregulated skin hydration-related markers, including hyaluronic acid (HA), transforming growth factor (TGF)-ß, and pro-collagen, in UVB-irradiated mice, compared with the vehicle control group. It also reduced UVB-induced wrinkle formation, collagen degradation, and epidermal thickness. Additionally, it remarkably suppressed the increased expression of matrix metalloproteinases (MMPs), and restored the activity of SOD and CAT in UVB-irradiated mice, compared with the vehicle control group. Furthermore, Oc. Ex treatment downregulated the production of inflammatory cytokines and phosphorylation of the mitogen-activated protein kinases (MAPKs) signaling pathway activated by UVB irradiation. CONCLUSION: This study revealed that Oc. Ex reduced skin thickness and the degradation of collagen fibers by increasing hydration markers and collagen-regulating factors in the skin of UVB-irradiated mice. It also inhibited UVB-induced antioxidant enzyme activity and inflammatory cytokine expression via MAPK signaling downregulation, suggesting that it prevents UVB-induced skin damage and photoaging, and has potential for clinical development in skin disease treatment.


Assuntos
Gafanhotos/química , Protetores contra Radiação/farmacologia , Envelhecimento da Pele/efeitos dos fármacos , Envelhecimento da Pele/efeitos da radiação , Animais , Catalase/metabolismo , Colágeno/metabolismo , Epiderme/efeitos dos fármacos , Epiderme/metabolismo , Humanos , Masculino , Metaloproteinases da Matriz/metabolismo , Camundongos , Camundongos Pelados , Proteínas Quinases Ativadas por Mitógeno/metabolismo , Superóxido Dismutase/metabolismo , Raios Ultravioleta/efeitos adversos
4.
Food Funct ; 10(9): 6121-6134, 2019 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-31497829

RESUMO

Collagen hydrolysate has been widely used as a nutraceutical agent against skin aging and has gained increasing attention. Previous research has suggested that oral administration of antioxidant collagen peptides (ACPs) exerted beneficial effects on the photoaging skin structure and collagen. However, the bioactive components in ACP metabolites that are responsible for the repair effects have not been elucidated. In this study, serum containing collagen peptides (CPS) after oral administration and collagen peptides isolated from serum metabolites (SCP) were collected and their effects on cell proliferation, hyaluronic acid secretion and the collagen synthesis pathway in UVA-induced skin fibroblasts (ESF) were evaluated. Furthermore, hydroxyproline (Hyp)-containing collagen peptides from SCP were analyzed and their repair effects were examined. The repair effects of ACP metabolites in serum differed depending on the preparation method and SCP were the active components responsible for the repair effects. SCP displayed repair effects by activating the TGF-ß/Smad pathway to promote procollagen synthesis and suppressing AP-1, MMP-1 and MMP-3 protein expression to prevent collagen degradation, in which SHCP exhibited the strongest bioactivity. In addition, SCP showed repair effects by reactive oxygen species (ROS) scavenging activity and preserving the endogenous antioxidant defense systems. Furthermore, IO (Ile-Hyp) and AOG (Ala-Hyp-Gly) were identified as the active peptides promoting procollagen synthesis by activating the TGF-ß/Smad3 pathway. These results may be useful in screening of anti-photoaging factors in metabolites and producing highly efficient collagen peptide products.


Assuntos
Colágeno/química , Colágeno/metabolismo , Proteínas de Peixes/química , Peptídeos/administração & dosagem , Envelhecimento da Pele/efeitos dos fármacos , Proteína Smad3/metabolismo , Fator de Crescimento Transformador beta/metabolismo , Animais , Antioxidantes/administração & dosagem , Antioxidantes/química , Carpas , Feminino , Fibroblastos/efeitos dos fármacos , Fibroblastos/metabolismo , Humanos , Metaloproteinase 3 da Matriz/genética , Metaloproteinase 3 da Matriz/metabolismo , Peptídeos/química , Ratos , Ratos Sprague-Dawley , Transdução de Sinais/efeitos dos fármacos , Envelhecimento da Pele/efeitos da radiação , Proteína Smad3/genética , Fator de Crescimento Transformador beta/genética , Raios Ultravioleta/efeitos adversos
5.
Oxid Med Cell Longev ; 2019: 6146942, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31531185

RESUMO

Background: Nanofat can protect against ultraviolet B- (UVB-) induced damage in nude mice. Fat extract (FE) is a cell-free fraction isolated from nanofat that is enriched with a variety of growth factors. Objective: To determine whether FE can protect against UVB-induced photoaging in cultured dermal fibroblasts and in nude mice. Method: For the in vitro study, human dermal skin fibroblasts were pretreated with FE 24 h prior to UVB irradiation. Generation of reactive oxygen species (ROS) was analyzed immediately following irradiation, while cell cycle analysis was performed 24 h after UVB irradiation. Senescence-associated ß-galactosidase (SA-ß-gal) expression, cell proliferation, and expression of glutathione peroxidase 1 (GPX-1), catalase, superoxide dismutase-1 (SOD-1), SOD-2, and collagen type 1 (COL-1) were investigated 72 h after UVB irradiation. For the in vivo study, the dorsal skin of nude mice was irradiated with UVB and mice were then treated with FE for 8 weeks. The thickness of the dermis, capillary density, and apoptotic cells in skin tissue sections were investigated after treatment. The expression of GPX-1, catalase, SOD-2, SOD-1, and COL-1 in the tissue was also measured. Result: FE significantly increased cell proliferation and protected cells against UVB-induced cell death and cell cycle arrest. FE reduced ROS and the number of aged cells induced by UVB irradiation. FE promoted the expression of COL-1 and GPX-1 in cultured dermal fibroblasts. FE treatment of UVB-irradiated skin increased dermal thickness and capillary density, decreased the number of apoptotic cells, and promoted the expression of COL-1 and GPX-1. Conclusion: FE protects human dermal fibroblasts and the skin of nude mice from UVB-induced photoaging through its antioxidant, antiapoptotic, and proangiogenic activities.


Assuntos
Tecido Adiposo/química , Misturas Complexas/farmacologia , Derme/metabolismo , Fibroblastos/metabolismo , Envelhecimento da Pele , Raios Ultravioleta/efeitos adversos , Animais , Senescência Celular/efeitos dos fármacos , Senescência Celular/efeitos da radiação , Misturas Complexas/química , Derme/patologia , Feminino , Fibroblastos/patologia , Humanos , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Nus , Oxirredutases/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Envelhecimento da Pele/efeitos dos fármacos , Envelhecimento da Pele/efeitos da radiação
6.
J Microbiol Biotechnol ; 29(9): 1349-1360, 2019 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-31474086

RESUMO

Chronic exposure to ultraviolet (UV) radiation, regarded as a major cause of extrinsic aging or photoaging characterized by wrinkle formation and skin dehydration, exerts adverse effects on skin by causing the overproduction of reactive oxygen species. Agastache rugosa Kuntze, known as Korean mint, possesses a wide spectrum of biological properties including antioxidation, anti-inflammation, and anti-atherosclerosis. Previous studies have reported that A. rugosa protected human keratinocytes against UVB irradiation by restoring the anti-oxidant defense system. However, the anti-photoaging effect of A. rugosa extract (ARE) in animal models has not yet been evaluated. ARE was orally administered to hairless mice at doses of 100 or 250 mg/kg/day along with UVB exposure for 12 weeks. ARE histologically improved UVB-induced wrinkle formation, epidermal thickening, erythema, and hyperpigmentation. In addition, ARE recovered skin moisture by improving skin hydration and transepidermal water loss (TEWL). Along with this, ARE increased hyaluronic acid levels by upregulating HA synthase genes. ARE markedly increased the density of collagen and the amounts of hydroxypoline via two pathways. First, ARE significantly downregulated the mRNA expression of matrix metalloproteinases responsible for collagen degradation by inactivating the mitogen-activated protein kinase/activator protein 1 pathway. Second, ARE stimulated the transforming growth factor beta/Smad signaling, consequently raising the mRNA levels of collagen-related genes. In addition, ARE not only increased the mRNA expression of antioxidant enzymes but also decreased inflammatory cytokines by blocking the protein expression of nuclear factor kappa B. Collectively, our findings suggest that A. rugosa may be a potential preventive and therapeutic agent for photoaging.


Assuntos
Agastache/química , Proteínas Quinases Ativadas por Mitógeno/metabolismo , Extratos Vegetais/farmacologia , Transdução de Sinais/efeitos dos fármacos , Envelhecimento da Pele/efeitos dos fármacos , Fator de Crescimento Transformador beta/metabolismo , Raios Ultravioleta/efeitos adversos , Animais , Antioxidantes/metabolismo , Colágeno/biossíntese , Colágeno/genética , Regulação da Expressão Gênica/efeitos dos fármacos , Mediadores da Inflamação/metabolismo , Camundongos Pelados , Proteínas Quinases Ativadas por Mitógeno/genética , Fosforilação/efeitos dos fármacos , Extratos Vegetais/administração & dosagem , Pele/efeitos da radiação , Envelhecimento da Pele/patologia , Envelhecimento da Pele/efeitos da radiação , Proteínas Smad/genética , Proteínas Smad/metabolismo , Fator de Transcrição AP-1/genética , Fator de Transcrição AP-1/metabolismo , Fator de Crescimento Transformador beta/genética
7.
Mar Drugs ; 17(8)2019 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-31374828

RESUMO

Excessive exposure to ultraviolet (UV) radiation is the main risk factor to develop skin pathologies or cancer because it encourages oxidative condition and skin inflammation. In this sense, strategies for its prevention are currently being evaluated. Natural products such as carotenoids or polyphenols, which are abundant in the marine environment, have been used in the prevention of oxidative stress due to their demonstrated antioxidant activities. Nevertheless, the anti-inflammatory activity and its implication in photo-prevention have not been extensively studied. Thus, we aimed to evaluate the combination of fucoxanthin (FX) and rosmarinic acid (RA) on cell viability, apoptosis induction, inflammasome regulation, and anti-oxidative response activation in UVB-irradiated HaCaT keratinocytes. We demonstrated for the first time that the combination of FX and RA (5 µM RA plus 5 µM FX, designated as M2) improved antioxidant and anti-inflammatory profiles in comparison to compounds assayed individually, by reducing UVB-induced apoptosis and the consequent ROS production. Furthermore, the M2 combination modulated the inflammatory response through down-regulation of inflammasome components such as NLRP3, ASC, and Caspase-1, and the interleukin (IL)-1ß production. In addition, Nrf2 and HO-1 antioxidant genes expression increased in UVB-exposed HaCaT cells pre-treated with M2. These results suggest that this combination of natural products exerts photo-protective effects by down-regulating NRLP3-inflammasome and increasing Nrf2 signalling pathway.


Assuntos
Anti-Inflamatórios/farmacologia , Antioxidantes/farmacologia , Cinamatos/farmacologia , Depsídeos/farmacologia , Envelhecimento da Pele/efeitos dos fármacos , Xantofilas/farmacologia , Apoptose/efeitos dos fármacos , Apoptose/efeitos da radiação , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos da radiação , Sinergismo Farmacológico , Humanos , Inflamassomos/efeitos dos fármacos , Inflamassomos/imunologia , Inflamassomos/metabolismo , Queratinócitos/efeitos dos fármacos , Queratinócitos/imunologia , Queratinócitos/efeitos da radiação , Fator 2 Relacionado a NF-E2/metabolismo , Proteína 3 que Contém Domínio de Pirina da Família NLR/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Transdução de Sinais/efeitos dos fármacos , Transdução de Sinais/imunologia , Transdução de Sinais/efeitos da radiação , Pele/citologia , Pele/efeitos dos fármacos , Pele/efeitos da radiação , Envelhecimento da Pele/efeitos da radiação , Raios Ultravioleta/efeitos adversos
8.
Int J Mol Med ; 44(2): 559-568, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31198982

RESUMO

Processed products from agricultural produce generate a large number of agricultural by­products that contain a number of functional substances. These are often discarded owing to the lack of suitable processing methods. The present study investigated the anti­photoaging properties of fermented rice bran (FRB), soybean cake (FSB) and sesame seed cake (FSC) on ultraviolet B (UVB)­irradiated hairless mouse skin. Results indicated that the oral administration of FRB, FSB and FSC effectively inhibited the UVB irradiation­induced expression of matrix metalloproteinase (MMP)­2, MMP­9, MMP­3 and MMP­13. Reverse transcription­quantitative polymerase chain reaction results also demonstrated that FRB, FSB and FSC significantly inhibited the UVB­induced expression of the genes encoding tumor necrosis factor­α, inducible nitric oxide synthase, interleukin (IL)­6 and IL­1ß when compared with the UVB­vehicle group (P<0.05). Additionally, collagen degradation and mast cell infiltration were reduced in hairless mouse skin. Furthermore, UVB­induced wrinkle formation was also significantly reduced in mouse skin compared with the UVB­vehicle group (P<0.05). These results reveal that fermented agricultural by­products may serve as potential functional materials with anti­photoaging activities.


Assuntos
Extratos Vegetais/farmacologia , Envelhecimento da Pele/efeitos dos fármacos , Envelhecimento da Pele/efeitos da radiação , Protetores Solares/farmacologia , Raios Ultravioleta/efeitos adversos , Animais , Produtos Agrícolas/química , Feminino , Fermentação , Camundongos Pelados , Oryza/química , Extratos Vegetais/química , Sesamum/química , Pele/efeitos dos fármacos , Pele/efeitos da radiação , Pele/ultraestrutura , Soja/química , Protetores Solares/química
9.
Int J Radiat Biol ; 95(7): 1043-1049, 2019 07.
Artigo em Inglês | MEDLINE | ID: mdl-31157572

RESUMO

In the 60 years since the inaugural edition of the International Journal of Radiation Biology, much of our understanding of the biological effects of solar radiation has changed. Earlier in the century, sunlight played a 'hero's' role in reducing disabling rickets, while today debate still continues on the amount of sun required before exposure reveals the 'villainous' side of solar radiation. Although knowledge of the ultra violet (UV) component of sunlight as a carcinogen has become widespread, skin cancer rates are still rising yearly. Twentieth century attitudes have seen an about-face in the field of dermatological sun protection, with sunscreens changing from recipes designed to promote a 'healthy tan' to formulations proven to block both ultraviolet B (UVB) and more recently, ultraviolet A (UVA), to minimize premature sun-aging and skin cancer risk. In the early 1960s, DNA was first found to exist within mitochondria, while recently the connections between mitochondrial changes and UV radiation exposure have been expanded. Sixty years ago, understanding of the endocrine systems of mammals was enjoying its infancy. Early discoveries that light, particularly natural light, could have profound effects on functions such as sleep patterns and hormonal balance were made, while today more advanced knowledge has led to lighting improvements having pronounced effects on human wellbeing. Photosensitization 60 years ago was a health concern for both humans and their domestic animals, while today chemically engineered photosensitizing drugs can be administered along with highly directed light to pinpoint delivery targets for drug action. Life on earth is inextricably bound up with solar radiation. This article attempts to outline many of the ways in which our opinions about solar radiation have changed since the journal's inception.


Assuntos
Radiobiologia/história , Luz Solar , Raios Ultravioleta , Animais , DNA/efeitos da radiação , Dano ao DNA , DNA Mitocondrial/metabolismo , História do Século XX , História do Século XXI , Humanos , Saúde Mental , Mitocôndrias/efeitos da radiação , Neoplasias/etiologia , Neoplasias/radioterapia , Fármacos Fotossensibilizantes , Raquitismo/radioterapia , Pele/efeitos da radiação , Envelhecimento da Pele/efeitos da radiação , Neoplasias Cutâneas/etiologia , Neoplasias Cutâneas/radioterapia , Vitamina D/metabolismo
10.
Oxid Med Cell Longev ; 2019: 9679731, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31073356

RESUMO

Ethnopharmacological Relevance. Penthorum chinense Pursh (Penthoraceae) is a traditional herbal plant that has been used in China for the treatment of jaundice, cholecystitis, edema, and infectious hepatitis. In addition, the Korea Medicinal Plant Dictionary states that Penthorum chinense Pursh can be used to treat contusions and skin bruises by improving blood flow. Recent studies have shown that Penthorum chinense Pursh ethanol extract (Pc-EE) exhibits strong antioxidant effects. In this study, we examined the effects of Pc-EE on UVB-induced or H2O2-induced oxidative stress, as well as its antimelanogenic properties. Cell viability, matrix metalloproteinase (MMP) expression, cyclooxygenease-2 (COX-2), and interleukin-6 (IL-6) expression and moisturizing factors were investigated in keratinocytes. Collagen synthesis induction was measured in HEK293T cells. For melanogenesis, the effects of Pc-EE on melanin content and tyrosinase activity were measured. Additionally, the antimelanogenic- and autophagy-inducing activities of Pc-EE were examined using immunoblotting and confocal microscopy. Pc-EE protected HaCaT cells against death from UVB irradiation- or H2O2-induced oxidative stress. Pc-EE increased the promoter activity of the type 1 procollagen gene Col1A1 and decreased the expression of MMPs, COX-2, IL-6, and hyaluronidase induced by UVB irradiation- or H2O2-induced oxidative stress. Pc-EE showed a strong antioxidant effect in the DPPH assay. In α-melanocyte-stimulating hormone- (α-MSH-) stimulated B16F10 cells, Pc-EE reduced melanin production, decreased tyrosinase expression and microphthalmia-associated transcription factor (MITF) protein levels, and decreased the phosphorylation levels of p38 and JNK. In HEK293T cells, Pc-EE promoted the expression of GFP-LC3B. In B16F10 cells, the LC3B and melanin contents were reduced by Pc-EE and were restored by the autophagy inhibitor 3-methyladenine (3-MA). These results suggest that Pc-EE can be used as a skin protection agent due to its antiapoptotic, antiaging, anti-inflammatory, and antimelanogenic properties.


Assuntos
Antioxidantes/farmacologia , Autofagia/efeitos dos fármacos , Etanol/química , Melaninas/antagonistas & inibidores , Extratos Vegetais/farmacologia , Saxifragaceae/química , Envelhecimento da Pele/efeitos dos fármacos , Animais , Apoptose/efeitos dos fármacos , Apoptose/efeitos da radiação , Autofagia/efeitos da radiação , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos da radiação , Colágeno/metabolismo , Humanos , Peróxido de Hidrogênio/toxicidade , Inflamação/patologia , Melanoma Experimental/patologia , Camundongos , Oxirredução , Transdução de Sinais/efeitos dos fármacos , Envelhecimento da Pele/efeitos da radiação , Raios Ultravioleta , alfa-MSH/farmacologia
11.
Molecules ; 24(9)2019 May 09.
Artigo em Inglês | MEDLINE | ID: mdl-31075987

RESUMO

Ultraviolet (UV) light-induced wrinkle formation is a major dermatological problem and is associated with alteration in collagen. Here, we investigated the potential of α-ionone, a naturally occurring aromatic compound, in regulation of UVB-induced photoaging in human Hs68 dermal fibroblasts and identified the mechanisms involved. We found that in human dermal fibroblasts, α-ionone inhibited UVB-induced loss of collagen. α-Ionone upregulated the molecules participating in the TGF-ß-SMAD pathway (TGF-ß1, phospho-SMAD2/3, Col1A1, and Col1A2), but downregulated the molecules involved in the MAPK-AP-1 signaling pathway (phospho-p38, phospho-JNK, phospho-ERK, phospho-c-Fos, phospho-c-Jun, MMP1, MMP3, and MMP9), in human dermal fibroblasts. α-Ionone treatment also increased hyaluronic acid contents, and this effect was accompanied by an upregulation of mRNA expression of genes (HAS1 and HAS2) involved in hyaluronic acid synthesis. Thus, α-ionone is effective in the prevention of UVB-induced decrease of collagen and hyaluronic acid in human dermal fibroblasts. We propose that α-ionone may prove beneficial for the prevention of UV-induced wrinkle formation and skin damage.


Assuntos
Derme/patologia , Fibroblastos/efeitos dos fármacos , Fibroblastos/efeitos da radiação , Norisoprenoides/farmacologia , Envelhecimento da Pele/efeitos dos fármacos , Envelhecimento da Pele/efeitos da radiação , Raios Ultravioleta , Colágeno/metabolismo , Fibroblastos/metabolismo , Humanos , Ácido Hialurônico/metabolismo , Modelos Biológicos , Norisoprenoides/química , Regulação para Cima/efeitos dos fármacos
12.
J Photochem Photobiol B ; 196: 111511, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31129510

RESUMO

Prolonged exposure of the skin to ultraviolet radiation (UV) leads to its damage and loss of protective properties. This condition called photoaging of the skin is caused by a number of destructive factors, such as reactive oxygen species (ROS) and proteolytic enzymes that cause damage to the extracellular matrix, e.g. collagen fibers. Many cells of the immune system, including neutrophils, are involved in the photoaging process. The presence of neutrophils in the skin exposed to UV irradiation is known; however, the mechanism of neutrophil activity at these conditions remains unclear. In our study, we focused on the ability of neutrophils to release neutrophil extracellular traps (NETs) and the role of these structures in the photoaging process. NET release occurs in response to various stimuli; however, we hereby showed that the UVA and UVB radiation that reaches the Earth's surface could activate the mechanism of netosis. UV-induced netosis was much faster than that activated by chemical or biological factors; however, it also occurred due to the production of ROS, known signal mediators in netosis. In this work, we also identified the probable netosis signaling pathway involved in the neutrophil response to UV. The participation of NET components may explain the ongoing process of skin photoaging, but it is also important to indicate netosis as a potential target for skin protection therapy. Antioxidants tested in this work, such as N-acetylcysteine, ethamsylate, as well as vitamin B1 (thiamine), can successfully inhibit UV-induced netosis, and thus be used as protective components against the negative effects of solar radiation.


Assuntos
Armadilhas Extracelulares/metabolismo , Neutrófilos/efeitos da radiação , Raios Ultravioleta , Antioxidantes/farmacologia , Apoptose/efeitos dos fármacos , Apoptose/efeitos da radiação , Células Cultivadas , Armadilhas Extracelulares/química , Regulação da Expressão Gênica/efeitos dos fármacos , Regulação da Expressão Gênica/efeitos da radiação , Humanos , Interleucinas/genética , Interleucinas/metabolismo , Proteína Quinase 1 Ativada por Mitógeno/metabolismo , Proteína Quinase 3 Ativada por Mitógeno/metabolismo , Neutrófilos/citologia , Neutrófilos/metabolismo , Fosforilação/efeitos da radiação , Espécies Reativas de Oxigênio/metabolismo , Transdução de Sinais/efeitos dos fármacos , Envelhecimento da Pele/efeitos da radiação , Quinase Syk/metabolismo
13.
J Food Sci ; 84(6): 1600-1608, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31132143

RESUMO

Considering the anti-photoaging effect of antioxidant compounds, we investigated the protective capacity of grape peel extract (GPE) and resveratrol on ultraviolet (UV)-induced skin wrinkle formation. Total phenolic, total anthocyanin, and total flavonoid content in GPE prepared from peel of Campbell Early variety were 23.96 ± 0.09, 3.27 ± 0.40, and 1.24 ± 0.09 mg/g dry weight, respectively. Additionally, trans-resveratrol and piceid content of the resulting GPE were 117.14 ± 19.97 and 85.23 ± 8.89 µg/g dry weight, respectively. Oral administration of either 2 g GPE or 2 mg resveratrol per kg body weight in mice attenuated UVB-induced epidermal thickening (the thickness was reduced by about 63% and 55% with GPE and resveratrol consumption prior to exposure to UVB, respectively, compared to only UVB-treated condition) and had marginally protective effect on wrinkle formation of skin exposed to UVB. As introduction of either GPE or resveratrol induced Nrf2-dependent antioxidant enzymes including heme oxygenase-1 (HO-1) in liver and skin as well as inhibited metalloproteinases, it is highly probable that the extract or resveratrol mitigated UVB-induced photoaging through activation of Nrf2/HO-1 signaling pathway. PRACTICAL APPLICATION: This study proved that resveratrol and the extract of grape peel, a common by-product of grape juice processing, provide effective protection from UV-induced skin wrinkle formation. Therefore, grape peel extract, which contains an appreciable amount of bioactive compound resveratrol, can be utilized as functional food ingredient for the manufacture of inner beauty products.


Assuntos
Heme Oxigenase-1/metabolismo , Fator 2 Relacionado a NF-E2/metabolismo , Extratos Vegetais/administração & dosagem , Resveratrol/administração & dosagem , Envelhecimento da Pele/efeitos dos fármacos , Vitis/química , Animais , Antioxidantes/administração & dosagem , Modelos Animais de Doenças , Feminino , Flavonoides/administração & dosagem , Flavonoides/análise , Frutas/química , Glucosídeos/administração & dosagem , Glucosídeos/análise , Heme Oxigenase-1/genética , Humanos , Camundongos , Camundongos Pelados , Fator 2 Relacionado a NF-E2/genética , Resveratrol/análise , Transdução de Sinais/efeitos dos fármacos , Transdução de Sinais/efeitos da radiação , Envelhecimento da Pele/genética , Envelhecimento da Pele/efeitos da radiação , Estilbenos/administração & dosagem , Estilbenos/análise , Raios Ultravioleta
14.
Biochem J ; 476(10): 1387-1400, 2019 05 21.
Artigo em Inglês | MEDLINE | ID: mdl-31036716

RESUMO

Ultraviolet-B exposure causes an inflammatory response, photoaged skin, and degradation of extracellular matrix proteins including collagen and elastin. The regulation of these genes was suggested as an important mechanism to attenuate skin aging. Glycolic acid (GA) is commonly present in fruits and recently used to treat dermatological diseases. We reported that GA slows down cell inflammation and aging caused by UVB. Little is known about GA retarding the skin premature senescence or how to impede these events. To investigate the potential of GA to regulate the expression of MMPs and collagen, GA was topically applied onto human keratinocytes and the C57BL/6J mice dorsal skin. In the present study, we demonstrated that GA reduced UVB-induced type-I procollagen expression and secretory collagen levels. GA reverted and dose-dependently increased the level of aquaporin-3 (AQP3), the expression of which was down-regulated by UVB. The UV-induced MMP-9 level and activity were reduced by GA pre-treatment. Concomitantly, GA reverted mitogen-activated protein kinase (MMP-9) activation and inhibited the extracellular signal-regulated kinase activation (p38, pERK) triggered by UVB. The animal model also presented that GA attenuated the wrinkles caused by UVB on the mouse dorsal skin. Finally, GA triggers the transient receptor potential vanilloid-1 (TRPV-1) channel to initiate the anti-photoaging mechanism in keratinocytes. These findings clearly indicated that the mechanisms of GA promote skin protection against UVB-induced photoaging and wrinkle formation. GA might be an important reagent and more widely used to prevent UVB-induced skin aging.


Assuntos
Aquaporina 3/biossíntese , Colágeno/metabolismo , Regulação da Expressão Gênica , Glicolatos/farmacologia , Queratinócitos , Metaloproteinase 9 da Matriz/química , Envelhecimento da Pele , Pele , Raios Ultravioleta , Administração Tópica , Animais , Regulação da Expressão Gênica/efeitos dos fármacos , Regulação da Expressão Gênica/efeitos da radiação , Queratinócitos/metabolismo , Queratinócitos/patologia , Sistema de Sinalização das MAP Quinases/efeitos dos fármacos , Sistema de Sinalização das MAP Quinases/efeitos da radiação , Camundongos , Pele/metabolismo , Pele/patologia , Envelhecimento da Pele/efeitos dos fármacos , Envelhecimento da Pele/patologia , Envelhecimento da Pele/efeitos da radiação , Proteínas Quinases p38 Ativadas por Mitógeno/metabolismo
15.
Mol Med Rep ; 20(1): 763-770, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31115540

RESUMO

Derivatives of caffeoylquinic acid (CQA) have been studied and reported as potent bioactive molecules possessing various health benefits including antioxidant and anti­inflammatory activities. In the present study, the protective effect of 3,5­dicaffeoyl­epi­quinic acid (DCEQA) isolated from Atriplex gmelinii on UVB­induced damages was investigated in human HaCaT keratinocytes. The effect of DCEQA against UVB­induced oxidative stress­mediated damages was determined measuring its ability to alleviate UVB­induced elevation of oxidative stress, proinflammatory response and antioxidant enzyme suppression through nuclear factor­like 2 (Nrf2). Treatment with DCEQA hindered the generation of intracellular reactive oxygen species. Increased levels of proinflammatory cytokines TNF­α, COX­2, IL­6 and IL­1ß following UVB exposure were suppressed by the introduction of DCEQA. Additionally, DCEQA upregulated the mRNA and protein expression of antioxidant enzymes superoxide dismutase­1 and heme oxygenase­1 which were inhibited under UVB irradiation. Antioxidant enzyme regulation transcription factor Nrf2 was also upregulated in the presence of DCEQA. These results suggest that DCEQA prevents photoaging via protection of keratinocytes from UVB irradiation by ameliorating the oxidative stress and pro­inflammatory response.


Assuntos
Queratinócitos/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Ácido Quínico/análogos & derivados , Envelhecimento da Pele/efeitos dos fármacos , Antioxidantes/farmacologia , Linhagem Celular , Heme Oxigenase-1/genética , Humanos , Queratinócitos/efeitos da radiação , Fator 2 Relacionado a NF-E2/genética , Ácido Quínico/farmacologia , Espécies Reativas de Oxigênio , Envelhecimento da Pele/genética , Envelhecimento da Pele/efeitos da radiação , Superóxido Dismutase/genética , Fator de Necrose Tumoral alfa/genética , Raios Ultravioleta
16.
J Med Food ; 22(8): 841-850, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31094612

RESUMO

Skin aging is associated with increased reactive oxygen species (ROS) produced by human cells. These radicals are the main causes of skin aging, and skin cells have developed antioxidant enzymes for protection against ROS-induced damage. Antioxidants play critical roles to prevent ROS-induced aging symptoms. In this study, the antioxidant properties of Pourthiaea villosa (Thunb.) Decne. extract (PVDE) were studied. Human dermal fibroblast (HDF) cells were treated with PVDE to evaluate its antioxidant and antiaging activities and to investigate the underlying mechanisms. The identified compounds were polyols, and phenolic and flavonoid compounds from PVDE by UHPLC-LTQ-IT-MS/MS. PVDE exhibited significant antioxidant effects, as evaluated with reducing power, and ABTS and DPPH radical scavenging activity. Furthermore, PVDE treatment significantly increased antioxidant enzyme expressions and effectively blocked H2O2-induced matrix metalloproteinase activity through MAPK signaling pathways in HDFs. Therefore, these results showed that PVDE affords an advantage of being a functional natural material with antioxidant and antiaging effects for the skin.


Assuntos
Fibroblastos/efeitos dos fármacos , Peróxido de Hidrogênio/efeitos adversos , Photinia/química , Extratos Vegetais/farmacologia , Envelhecimento da Pele/efeitos dos fármacos , Protetores Solares/farmacologia , Antioxidantes/farmacologia , Fibroblastos/metabolismo , Humanos , Metaloproteinases da Matriz/genética , Metaloproteinases da Matriz/metabolismo , Extratos Vegetais/química , Espécies Reativas de Oxigênio/metabolismo , Envelhecimento da Pele/efeitos da radiação , Protetores Solares/química , Raios Ultravioleta/efeitos adversos
17.
J Cosmet Dermatol ; 18(3): 815-820, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30964240

RESUMO

BACKGROUND: The skin exposome refers to the constellation of external exposures that contribute to cutaneous aging, including solar radiation, air pollution, tobacco smoke, unbalanced nutrition, and cosmetic products. This review explores the skin exposome and the role of a combination hyaluronic acid and mineralized thermal water product used to restore and maintain optimal skin barrier function. METHOD: An expert panel of 7 dermatologists who treat clinical signs of facial aging convened for a one-day meeting to discuss the results of a literature review on the skin exposome and the role of M89, a mineralized thermal water and hyaluronic acid-based gel, to improve the quality of facial skin. Evidence coupled with expert opinion and experience of the panel was used to address clinical challenges in the treatment of photo-aging, and the use of M89. RESULTS: Solar radiation (ultraviolet radiation, visible light, and infrared radiation), air pollution, tobacco smoke, nutrition, and miscellaneous factors, including stress, sleep deprivation, and temperature, may potentiate skin aging by triggering molecular processes that damage skin structure. M89 was developed to maintain and restore skin and contains ingredients to aid physical, hydric, antioxidant, and antimicrobial skin barrier function. CONCLUSIONS: Increasing knowledge of the exposome and microenvironment contributing to skin aging may support a better understanding of measures to support the skin. The initial results of in vitro and clinical studies of M89 show its potential to improve skin barrier function.


Assuntos
Cosméticos/administração & dosagem , Exposição Ambiental/efeitos adversos , Ácido Hialurônico/administração & dosagem , Envelhecimento da Pele/efeitos dos fármacos , Água/administração & dosagem , Poluição do Ar/efeitos adversos , Cosméticos/química , Face , Humanos , Pele/efeitos dos fármacos , Pele/metabolismo , Pele/efeitos da radiação , Absorção Cutânea/efeitos dos fármacos , Absorção Cutânea/efeitos da radiação , Envelhecimento da Pele/efeitos da radiação , Luz Solar/efeitos adversos , Água/química
18.
J Cosmet Dermatol ; 18(2): 511-516, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30950583

RESUMO

BACKGROUND: Laxity and rhytides are manifestations of photodamage on the face and chest. Nonablative radiofrequency (RF) is one of the most common procedures used for skin rejuvenation. AIM: The aim of this study was to assess the elasticity of face and chest skin after multipolar radiofrequency. PATIENTS/METHODS: Thirty women, aged 43-68, were included in the study. Twenty women were postmenopausal and 10 were premenopausal. They received 4 treatment sessions with an application of nonablative radiofrequency in 2-week intervals. Biomechanical properties of the skin were measured with the use of a Cutometer. RESULTS: The objective evaluation in a cutometric analysis showed a statistically significant improvement between measurements taken in the pretreatment period and 3 months after the treatment. CONCLUSIONS: The study is an objective confirmation that RF treatment improves skin elasticity. The method may appear to be beneficial for women of any age and skin types. It is a noninvasive treatment with a low risk of complications.


Assuntos
Técnicas Cosméticas , Elasticidade/efeitos da radiação , Terapia por Radiofrequência , Envelhecimento da Pele/efeitos da radiação , Pele/efeitos da radiação , Adulto , Idoso , Face , Estudos de Viabilidade , Feminino , Humanos , Pessoa de Meia-Idade , Rejuvenescimento , Tórax
19.
J Photochem Photobiol B ; 194: 166-173, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30981089

RESUMO

Ultraviolet (UV) light exposure-induced photoaging of the skin is a multifactorial process involving both extrinsic and intrinsic cellular mechanisms. Several naturally occurring products are known to confer protection against UV light-induced skin damage. Our preliminary studies confirmed that the ethyl acetate fraction of coffee silverskin exhibits inhibitory effects on matrix metalloproteases (MMPs). Furthermore, we previously isolated and identified atractyligenin, which has MMP-inhibitory activity, from the silverskin ethyl acetate fraction. The aim of this study was to elucidate the anti-photoaging effects of atractyligenin on human dermal fibroblasts and the underlying mechanism. Human dermal fibroblasts were exposed to 8 J/cm2 UVA radiation, and cell viability was analyzed by MTT assay. The fluorescent dye 2', 7'-dichlorodihydrofluorescein diacetate (H2DCF-DA) was used to measure the intracellular reactive oxygen species (ROS) levels. Our study showed that atractyligenin significantly suppressed the expression of UVA-induced MMPs by inhibiting intracellular ROS production. Atractyligenin treatment reduced c-Jun phosphorylation and c-Fos expression by inhibiting the mitogen-activated protein kinase (MAPK) signaling pathway activated by UVA irradiation. Additionally, treatment with atractyligenin contributed to the homeostasis of collagen by restoring the loss of collagen absorption-related receptor Endo180 and altered fibroblast morphology induced by UVA irradiation. These results indicate that atractyligenin isolated from coffee silverskin inhibits multiple pathways in the human skin photoaging process and is thus a potential candidate for treatment or prevention of photoaging.


Assuntos
Atractilosídeo/análogos & derivados , Café/química , Envelhecimento da Pele/efeitos dos fármacos , Envelhecimento da Pele/efeitos da radiação , Atractilosídeo/farmacologia , Sobrevivência Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos da radiação , Regulação para Baixo/efeitos dos fármacos , Regulação para Baixo/efeitos da radiação , Regulação da Expressão Gênica/efeitos dos fármacos , Regulação da Expressão Gênica/efeitos da radiação , Humanos , Proteínas Quinases JNK Ativadas por Mitógeno/metabolismo , Metaloproteinases da Matriz/metabolismo , Proteínas Quinases Ativadas por Mitógeno/metabolismo , Fosforilação/efeitos dos fármacos , Fosforilação/efeitos da radiação , Espécies Reativas de Oxigênio/metabolismo
20.
J Dermatol Sci ; 94(1): 220-228, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30956032

RESUMO

BACKGROUND: Ultraviolet A (UVA) radiation is the most relevant component of solar radiation-induced skin aging. Sunscreens were used to minimize the harmful effects of UV radiation on our skin by reducing UV irradiance. We previously found that at equivalent fluence, UVB radiation at low irradiance (LI) has higher photocarcinogenic potential as compared to its high irradiance (HI) counterpart. OBJECTIVES: To examine the effects of equivalent fluence of UVA radiation administered at different irradiance on photoaging. METHODS: Both the hairless mice (SKH-1) and human dermal fibroblasts were irradiated with high irradiance UVA (HIUVA) or low irradiance UVA (LIUVA; 50% irradiance of HIUVA) at equivalent fluence. Parameters related to skin photoaging were evaluated. RESULTS: For hairless mice receiving equivalent fluence of UVA radiation, LIUVA treated mice showed prominent skin aging as compared to its HIUVA treated counterpart. In addition, LIUVA radiation induced higher reactive oxygen species (ROS) production and c-Jun N-terminal kinases (JNK) phosphorylation as compared to their HIUVA treated counterparts. Pretreatment with N-acetylcysteine (NAC) abrogate the difference between HI and LIUVA radiation on fibroblasts in terms of intracellular ROS, JNK phosphorylation, MMP-1 expression and type I collagen expression. CONCLUSION: UVA radiation administered at LI (a scenario similar to sunscreen use) led to more severe aging process as compared to its HI counterpart. Unexpected negative effect may be imposed on the skin if sunscreen use is accompanied by longer duration spent under the sun.


Assuntos
Envelhecimento da Pele/efeitos da radiação , Pele/efeitos da radiação , Raios Ultravioleta/efeitos adversos , Animais , Relação Dose-Resposta à Radiação , Elasticidade/efeitos da radiação , Fibroblastos/metabolismo , Fibroblastos/efeitos da radiação , Humanos , Metaloproteinase 1 da Matriz/metabolismo , Camundongos , Camundongos Pelados , Modelos Animais , Espécies Reativas de Oxigênio/metabolismo , Pele/citologia , Pele/metabolismo , Envelhecimento da Pele/efeitos dos fármacos , Protetores Solares/administração & dosagem , Fatores de Tempo
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