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1.
Braz. j. oral sci ; 21: e226262, jan.-dez. 2022. ilus
Artigo em Inglês | LILACS, BBO - Odontologia | ID: biblio-1354997

RESUMO

Aim: To evaluate the impact of a dual-cured adhesive system on the in situ degree of conversion (DC), bond strength (BS) and failure mode (FM) of adhesive interfaces in dentin cavities restored with a bulk-fill resin composite. Methods: 4-mm-deep dentin cavities with a 3.1 C-factor were created in 68 bovine incisors (n = 17 per group). The lightcured (Scotchbond™ Universal) or the dual-cured (Adper™ Scotchbond™ Multi-purpose Plus) adhesive system was applied to the cavities, which were then restored with a bulkfill resin composite (Filtek™ Bulk Fill). In situ DC analysis was performed by means of micro Raman spectroscopy at the top and bottom interfaces. Push-out BS was measured in a universal testing machine after 24-h or 6-month water storage. FM was determined with a stereomicroscope. Data of in situ DC and BS were analyzed by two-way analysis of variance (ANOVA) and Tukey test (p<0.05), while the FM was analyzed descriptively. Results: The groups that received the dual-cured adhesive system showed statistically higher in situ DC and BS than those that received the light-cured adhesive system. Cohesive failure mode was the most frequent in all conditions. Conclusion: In situ DC and BS were influenced by the curing strategies of the adhesive systems with better performance of the dual-cured material


Assuntos
Envelhecimento , Adesivos Dentinários , Resinas Compostas , Fenômenos Físicos , Polimerização
2.
Braz. j. oral sci ; 21: e225454, jan.-dez. 2022. ilus
Artigo em Inglês | LILACS, BBO - Odontologia | ID: biblio-1366512

RESUMO

Aim: Evaluating the resin-dentin bond strength of Class II conventional and bulk-fill composite restorations, using different cavity sizes before and after aging. Methods: Seventy-five human molars were distributed into groups according to the buccolingual width of the cavities, conservative (n=25) and extended (n=50). They were divided according to the restorative material: conventional (Z100/control group) or bulk-fill resin composites (Filtek Bulk Fill/FBF; Tetric N Ceram Bulk Fill/TNCBF; Filtek Bulk Fill Flow/FBFF; Surefill SDR flow/SDR). The restored teeth were sectioned on sticks (n=50 per restorative materials + width cavities group), half were stored in Water/Ethanol 75% for 30 days and the other half were submitted to the immediate microtensile bond strength (µTBS) test. Data were analyzed applying the Three-Way Analysis of Variance (ANOVA), Bonferroni test, test t, and Weibull analyses (p<0.05). Results: SDR and FBF presented lower µTBSvalues for extended preparation when compared to the conservative preparation, before aging. After aging, only for the FBFF, a decrease in the µTBSvalues was observed. Comparing the µTBSvalues, before and after aging, the SDR demonstrated lower µTBSvalues after aging when the conservative cavity was used. A decrease in the µTBSvalues was observed for the Z100, the FBF and, the FBFF, after aging, when the extended cavity was used. Conclusion: The effect of cavity preparation and aging on the resin-dentin of Class II is material dependent. Most of the bulk-fill resin composites evaluated presented a similar performance to the conventional resin composites for all the conditions of this study


Assuntos
Humanos , Resistência à Tração , Envelhecimento , Resinas Compostas/análise , Preparo da Cavidade Dentária
3.
Rev. urug. enferm ; 17(2): 1-12, dic. 2022.
Artigo em Espanhol | LILACS, BDENF - Enfermagem, BNUY, BNUY-Enf | ID: biblio-1391808

RESUMO

La alimentación adecuada es un derecho humano que contribuye a una buena calidad de vida de las personas y uno de sus componentes básicos es la accesibilidad. En el Plan de cuidados de Enfermería, se valora el proceso de envejecimiento y las modifi caciones que éste genera pudiendo contribuir a situaciones de vulnerabilidad desde el punto de vista nutricional. La accesibilidad a la alimentación se puede ver afectada por diferentes factores, como es el acceso físico, alimentos sufi cientes y adecuados, y las dificultades económicas. El objetivo fue conocer la accesibilidad en la alimentación de personas mayores (65 años y más) que residen en complejos habitacionales para jubilados y pensionistas. Se realizó un estudio de tipo cuantitativo de corte transversal. La recolección de los datos se llevó a cabo en tres complejos habitacionales para jubilados y pensionistas, seleccionados dos de ellos en la ciudad de Montevideo y uno en la ciudad de Rivera. La muestra estuvo conformada por 68 personas (69% de los residentes) que residen en los complejos.Los resultados demuestran que un 18% de esta población tiene dificultades en la accesibilidad en la alimentación, las causas fueron la falta de dinero en un 70% y un 30% por problema en la movilidad. Es reconocido en Uruguay el derecho a la alimentación y en generar políticas públicas y programas que contribuyan a satisfacer esta necesidad vital y garantizar el acceso a la misma, en grupos de población vulnerables como puede ser en las personas mayores.


Adequate food is a human right that contributes to a good quality of life for people and one of its basic components is accessibility. In the Nursing Care Plan, the aging process and the changes it generates are valued and may contribute to situations of vulnerability from the nutritional point of view. Accessibility to food can be affected by different factors, such as physical access, sufficient and adequate food, and economic difficulties. The objective was to know the accessibility in food of older people (65 years and over) who reside in housing complexes for retirees and pensioners. A quantitative cross-sectional study was carried out. Data collection was carried out in three housing complexes for retirees and pensioners, two of them selected in the city of Montevideo and one in the city of Rivera. The sample consisted of 68 people (69% of the residents) who reside in the complexes. The results show that 18% of this population has difficulties in food accessibility, the causes were lack of money in 70% and 30% due to mobility problems. The right to food is recognized in Uruguay and to generate public policies and programs that contribute to satisfying this vital need and guaranteeing access to it, in vulnerable population groups such as the elderly.


A alimentação adequada é um direito humano que contribui para uma boa qualidade de vida das pessoas e um de seus componentes básicos é a acessibilidade. No Plano de Assistência de Enfermagem, o processo de envelhecimento e as mudanças que ele gera são valorizados e podem contribuir para situações de vulnerabilidade do ponto de vista nutricional. A acessibilidade aos alimentos pode ser afetada por diversos fatores, como acesso físico, alimentação suficiente e adequada e dificuldades econômicas. O objetivo foi conhecer a acessibilidade na alimentação de pessoas idosas (65 anos ou mais) que residem em conjuntos habitacionais para aposentados e pensionistas. Foi realizado um estudo quantitativo transversal. A coleta de dados foi realizada em três conjuntos habitacionais para aposentados e pensionistas, dois deles selecionados na cidade de Montevidéu e um nacidade de Rivera. A amostra foi composta por 68 pessoas (69% dos moradores) que residem nos complexos. Os resultados mostram que 18% dessa população tem dificuldades na acessibilidade alimentar, as causas foram falta de dinheiro em 70% e 30% por problemas de locomoção. O direito à alimentação é reconhecido no Uruguai e para gerar políticas e programas públicos que contribuam para satisfazer esta necessidade vital e garantir o acesso a ela, em grupos populacionais vulneráveis como os idosos.


Assuntos
Humanos , Masculino , Feminino , Idoso , Idoso de 80 Anos ou mais , Saúde do Idoso Institucionalizado , Nutrição do Idoso , Acesso a Alimentos Saudáveis , Fatores Socioeconômicos , Uruguai , Envelhecimento , Promoção da Saúde Alimentar e Nutricional , Estado Nutricional , Estudos Transversais , Cognição , Vulnerabilidade em Saúde , Limitação da Mobilidade
4.
Zhongguo Zhong Yao Za Zhi ; 47(15): 4119-4127, 2022 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-36046902

RESUMO

To explore the effect and mechanism of Dahuang Zhechong Pills(DHZCP), a classical prescription, in improving testicular aging(TA) in vivo, the authors randomly divided 24 male rats into four groups: the normal, model, DHZCP and vitamin E(VE) groups. The TA rat model was established by continuous gavage of D-galactose(D-gal). During the experiment, the rats in the DHZCP and VE groups were given DHZCP suspension and VE suspension, respectively by gavage, while those in the normal and model groups were gavaged saline separately every day. After the co-administration of D-gal and various drugs for 60 days, all rats were sacrificed, and their blood and testis were collected. Further, various indexes related to TA and necroptosis of testicular cells in the model rats were examined and investigated, which included the aging phenotype, total testicular weight, testicular index, histopathological features of testis, number of spermatogenic cells, sex hormone level, expression characteristics of reactive oxygen species(ROS) in testis, expression levels and characteristics of cyclins in testis, and protein expression levels of the key molecules in receptor-interacting serine/threonine-protein kinase 1(RIPK1)/receptor-interacting serine/threonine-protein kinase 3(RIPK3)/mixed lineage kinase domain like pseudokinase(MLKL) signaling pathway in each group. The results showed that, for the TA model rats, both DHZCP and VE improved their aging phenotype, total testicular weight, testicular index, pathological features of testis, number of spermatogenic cells, serum testosterone and follicle stimulating hormone levels, expression characteristics of ROS and protein expression levels and characteristics of P21 and P53 in testis. In addition, DHZCP and VE improved the protein expression levels of the key molecules in RIPK1/RIPK3/MLKL signaling pathway in testis of the model rats. Specifically, DHZCP was better than VE in the improvement of RIPK3. In conclusion, in this study, the authors found that DHZCP, similar to VE, ameliorated D-gal-induced TA in model rats in vivo, and its mechanism was related to reducing necroptosis of testicular cells by inhibiting the activation of RIPK1/RIPK3/MLKL signaling pathway. This study provided preliminary pharmacological evidence for the development and application of classical prescriptions in the field of men's health.


Assuntos
Necroptose , Testículo , Envelhecimento , Animais , Medicamentos de Ervas Chinesas , Masculino , Proteínas Quinases/genética , Ratos , Espécies Reativas de Oxigênio/metabolismo , Proteína Serina-Treonina Quinases de Interação com Receptores/genética , Proteína Serina-Treonina Quinases de Interação com Receptores/metabolismo , Proteína Serina-Treonina Quinases de Interação com Receptores/farmacologia , Serina/farmacologia , Transdução de Sinais , Treonina/farmacologia
5.
Nat Commun ; 13(1): 5187, 2022 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-36057685

RESUMO

Specific functions of the immune system are essential to protect us from infections caused by pathogens such as viruses and bacteria. However, as we age, the immune system shows a functional decline that can be attributed in large part to age-associated defects in hematopoietic stem cells (HSCs)-the cells at the apex of the immune cell hierarchy. Here, we find that the Hippo pathway coactivator TAZ is potently induced in old HSCs and protects these cells from functional decline. We identify Clca3a1 as a TAZ-induced gene that allows us to trace TAZ activity in vivo. Using CLCA3A1 as a marker, we can isolate "young-like" HSCs from old mice. Mechanistically, Taz acts as coactivator of PU.1 and to some extent counteracts the gradual loss of PU.1 expression during HSC aging. Our work thus uncovers an essential role for Taz in a previously undescribed fail-safe mechanism in aging HSCs.


Assuntos
Envelhecimento , Células-Tronco Hematopoéticas , Envelhecimento/fisiologia , Animais , Células-Tronco Hematopoéticas/metabolismo , Camundongos
6.
Respir Res ; 23(1): 229, 2022 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-36058907

RESUMO

BACKGROUND: Aging has been evidenced to bring about some structural and functional lung changes, especially in COPD. However, whether aging affects SAD, a possible precursor of COPD, has not been well characterized. OBJECTIVE: We aimed to comprehensively assess the relationship between aging and SAD from computed tomography, impulse oscillometry, and spirometry perspectives in Chinese. METHODS: We included 1859 participants from ECOPD, and used a linear-by-linear association test for evaluating the prevalence of SAD across various age subgroups, and multivariate regression models for determining the impact of age on the risk and severity of SAD. We then repeated the analyses in these subjects stratified by airflow limitation. RESULTS: The prevalence of SAD increases over aging regardless of definitional methods. After adjustment for other confounding factors, per 10-yrs increase in age was significantly associated with the risk of CT-defined SAD (OR 2.57, 95% CI 2.13 to 3.10) and the increase in the severity of air trapping (ß 2.09, 95% CI - 0.06 to 4.25 for LAA-856), airway reactance (ß - 0.02, 95% CI - 0.04 to - 0.01 for X5; ß 0.30, 95% CI 0.13 to 0.47 for AX; ß 1.75, 95% CI 0.85 to 2.66 for Fres), as well as the decrease in expiratory flow rates (ß - 3.95, 95% CI - 6.19 to - 1.71 for MMEF%predicted; ß - 5.42, 95% CI - 7.88 to - 2.95 for FEF50%predicted) for SAD. All these associations were generally maintained in SAD defined by IOS or spirometry. After stratification of airflow limitation, we further found that the effect of age on LAA-856 was the most significant among almost all subgroups. CONCLUSIONS: Aging is significantly associated with the prevalence, increased risk, as well as worse severity of SAD. CT may be a more optimal measure to assess aging-related SAD. The molecular mechanisms for the role of aging in SAD need to be explored in the future. Trial registration Chinese Clinical Trial Registry ChiCTR1900024643. Registered on 19 July 2019.


Assuntos
Doença Pulmonar Obstrutiva Crônica , Envelhecimento , Estudos Transversais , Humanos , Pulmão/diagnóstico por imagem , Doença Pulmonar Obstrutiva Crônica/diagnóstico , Doença Pulmonar Obstrutiva Crônica/epidemiologia , Espirometria
7.
Inquiry ; 59: 469580221121511, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36062304

RESUMO

The implications of population aging for economic growth is not only the shrinking working-age population, but also the increasing health care burden of the elderly population. It is difficult to explain clearly the relationship between a country's aging population and its economy without considering health effects. Based on the Solow economic growth model, the aims of this study are to estimate the economic effects of the health care burden for elderly population, and to access whether reducing effective labor input for economic production. The analysis employs a set of econometric approaches including fixed effects, generalized method of moments, instrumental variable, and mediation regression analyses using a multinational multi-database covering the years 2000-2019. The empirical evidence indicates that the health care burden was negatively correlated with economic growth during the study period, with every 1% increase in the health care burden leading to a 0.083% decrease in the GDP growth rate. The results of heterogeneity analysis and mediating analysis further confirmed that worsening health in the elderly population could be associated with the deceleration in economic development through the indirect pathway that lowering the employment rate of working-age population. This study provides new empirical evidence on the economic impact of population aging that the poor health of elderly population can be one critical factor of limiting economic growth, for the reason the labor inputs in household production are likely crowded out by family caregiving.


Assuntos
Fardo do Cuidador , Países em Desenvolvimento , Idoso , Envelhecimento , Emprego , Humanos , Dinâmica Populacional , Fatores Socioeconômicos
8.
J Transl Med ; 20(1): 406, 2022 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-36064418

RESUMO

BACKGROUND: Glucocorticoid signalling is closely related to both epilepsy and associated cognitive impairment, possibly through mechanisms involving neuronal apoptosis. As a critical enzyme for glucocorticoid action, the role of 11ß-hydroxysteroid dehydrogenase 1 (11ß-HSD1) in epileptogenesis and associated cognitive impairment has not previously been studied. METHODS: We first investigated the expression of 11ß-HSD1 in the pentylenetetrazole (PTZ) kindling mouse model of epilepsy. We then observed the effect of overexpressing 11ß-HSD1 on the excitability of primary cultured neurons in vitro using whole-cell patch clamp recordings. Further, we assessed the effects of adeno-associated virus (AAV)-induced hippocampal 11ß-HSD1 knockdown in the PTZ model, conducting behavioural observations of seizures, assessment of spatial learning and memory using the Morris water maze, and biochemical and histopathological analyses. RESULTS: We found that 11ß-HSD1 was primarily expressed in neurons but not astrocytes, and its expression was significantly (p < 0.05) increased in the hippocampus of PTZ epilepsy mice compared to sham controls. Whole-cell patch clamp recordings showed that overexpression of 11ß-HSD1 significantly decreased the threshold voltage while increasing the frequency of action potential firing in cultured hippocampal neurons. Hippocampal knockdown of 11ß-HSD1 significantly reduced the severity score of PTZ seizures and increased the latent period required to reach the fully kindled state compared to control knockdown. Knockdown of 11ß-HSD1 also significantly mitigated the impairment of spatial learning and memory, attenuated hippocampal neuronal damage and increased the ratio of Bcl-2/Bax, while decreasing the expression of cleaved caspase-3. CONCLUSIONS: 11ß-HSD1 participates in the pathogenesis of both epilepsy and the associated cognitive impairment by elevating neuronal excitability and contributing to apoptosis and subsequent hippocampal neuronal damage. Inhibition of 11ß-HSD1, therefore, represents a promising strategy to treat epilepsy and cognitive comorbidity.


Assuntos
Disfunção Cognitiva , Epilepsia , 11-beta-Hidroxiesteroide Desidrogenase Tipo 1/genética , 11-beta-Hidroxiesteroide Desidrogenase Tipo 1/metabolismo , Envelhecimento , Animais , Apoptose , Disfunção Cognitiva/complicações , Epilepsia/complicações , Epilepsia/genética , Glucocorticoides , Aprendizagem em Labirinto/fisiologia , Camundongos , Convulsões/genética
9.
PLoS One ; 17(9): e0274212, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36067136

RESUMO

Age-related changes in brain structure include atrophy of the brain parenchyma and white matter changes of presumed vascular origin. Enlargement of the ventricles may occur due to atrophy or impaired cerebrospinal fluid (CSF) circulation. The co-occurrence of these changes in neurodegenerative diseases and in aging brains often requires investigators to take both into account when studying the brain, however, automated segmentation of enlarged ventricles and white matter hyperintensities (WMHs) can be a challenging task. Here, we present a hybrid multi-atlas segmentation and convolutional autoencoder approach for joint ventricle parcellation and WMH segmentation from magnetic resonance images (MRIs). Our fully automated approach uses a convolutional autoencoder to generate a standardized image of grey matter, white matter, CSF, and WMHs, which, in conjunction with labels generated by a multi-atlas segmentation approach, is then fed into a convolutional neural network to parcellate the ventricular system. Hence, our approach does not depend on manually delineated training data for new data sets. The segmentation pipeline was validated on both healthy elderly subjects and subjects with normal pressure hydrocephalus using ground truth manual labels and compared with state-of-the-art segmentation methods. We then applied the method to a cohort of 2401 elderly brains to investigate associations of ventricle volume and WMH load with various demographics and clinical biomarkers, using a multiple regression model. Our results indicate that the ventricle volume and WMH load are both highly variable in a cohort of elderly subjects and there is an independent association between the two, which highlights the importance of taking both the possibility of enlarged ventricles and WMHs into account when studying the aging brain.


Assuntos
Leucoaraiose , Substância Branca , Idoso , Envelhecimento , Atrofia/patologia , Encéfalo/diagnóstico por imagem , Encéfalo/patologia , Humanos , Processamento de Imagem Assistida por Computador , Imageamento por Ressonância Magnética/métodos , Substância Branca/diagnóstico por imagem , Substância Branca/patologia
10.
PLoS One ; 17(9): e0273766, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36067173

RESUMO

Cancer cachexia is accompanied by muscle atrophy, sharing multiple common catabolic pathways with sarcopenia, including mitochondrial dysfunction. This study investigated gene expression from skeletal muscle tissues of older healthy adults, who are at risk of age-related sarcopenia, to identify potential gene biomarkers whose dysregulated expression and protein interference were involved in non-small cell lung cancer (NSCLC). Screening of the literature resulted in 14 microarray datasets (GSE25941, GSE28392, GSE28422, GSE47881, GSE47969, GSE59880 in musculoskeletal ageing; GSE118370, GSE33532, GSE19804, GSE18842, GSE27262, GSE19188, GSE31210, GSE40791 in NSCLC). Differentially expressed genes (DEGs) were used to construct protein-protein interaction networks and retrieve clustering gene modules. Overlapping module DEGs were ranked based on 11 topological algorithms and were correlated with prognosis, tissue expression, and tumour purity in NSCLC. The analysis revealed that the dysregulated expression of the mammalian mitochondrial ribosomal proteins, Mitochondrial Ribosomal Protein S26 (MRPS26), Mitochondrial Ribosomal Protein S17 (MRPS17), Mitochondrial Ribosomal Protein L18 (MRPL18) and Mitochondrial Ribosomal Protein L51 (MRPL51) were linked to reduced survival and tumour purity in NSCLC while tissue expression of the same genes followed an opposite direction in healthy older adults. These results support a potential link between the mitochondrial ribosomal microenvironment in ageing muscle and NSCLC. Further studies comparing changes in sarcopenia and NSCLC associated cachexia are warranted.


Assuntos
Carcinoma Pulmonar de Células não Pequenas , Neoplasias Pulmonares , Sarcopenia , Idoso , Envelhecimento/genética , Caquexia/patologia , Carcinoma Pulmonar de Células não Pequenas/patologia , Perfilação da Expressão Gênica/métodos , Regulação Neoplásica da Expressão Gênica , Homeostase , Humanos , Neoplasias Pulmonares/patologia , Mitocôndrias/metabolismo , Proteínas Ribossômicas/genética , Sarcopenia/genética , Sarcopenia/patologia , Microambiente Tumoral
11.
Nihon Ronen Igakkai Zasshi ; 59(3): 275-283, 2022.
Artigo em Japonês | MEDLINE | ID: mdl-36070899

RESUMO

In Japan, the ageing population is increasing, and the issue of "the declining birthrate and increasing ageing population", "population declining", and "extending healthy life expectancy" have been taken up in various academic fields, but they are still unsolved in the term of effective methods. Currently, in each region of Japan unlike big cities such as Tokyo and Osaka, there is the shortage of medical doctors, hospitals, living supportive infrastructure of roads, communications, public transportation, banks, post offices, and supermarkets, etc. All the necessary infrastructure is collapsing, and the current situation is becoming apparent in population declining villages of Japan. Will our country become more concentrated in big cities in the future? In particular, it is the elderly living in the community who are directly affected by these factors. In this article, we would like to introduce the history and recent activities of "Age-friendly cities and communities (AFCC)" led by the World Health Organization (WHO), and also introduce the efforts of "The Ageing in Place in Cities" project by Manchester Urban Ageing Research Group, the University of Manchester, UK, Akita City Hall, and Advanced Research Center for Geriatric and Gerontology (ARGG), Akita University, Japan.


Assuntos
Geriatria , Vida Independente , Idoso , Envelhecimento , Cidades , Humanos , Japão , Reino Unido , Universidades
12.
PLoS One ; 17(9): e0274210, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36054131

RESUMO

Increased resting systemic anti-inflammatory responses have previously been reported after a period of progressive resistance training (PRT) with daily consumption of a high-protein dairy beverage. The study aimed to investigate the independent and combined effects of consuming a high protein dairy milk beverage with or without a PRT on markers of intestinal epithelial integrity and selected systemic inflammatory responses in active older (≥50 yrs) adults. Thirty two (males n = 24, females n = 8) active older adults [mean (SD): Age 62 (7) years, weight 74.2 (14.0) kg, height 1.73 (10.0) cm, BMI 24.9 (4.0) kg/m2, and body fat mass: 25.8 (9.1)%)], that reported exercising ≥3/week (211 (91) min/week) were randomly allocated into one of four groups: dairy milk (DM), exercise and dairy milk (EX+DM), exercise alone (EX), and control (CON). Groups with EX underwent 12-weeks whole-body PRT program (x3 sessions/week), groups with DM consumed the beverage twice daily (30g protein/day), and CON was required to carry out their ad libitum dietary and exercise habits. Plasma concentrations of CRP, IL-1ß, IL-1ra, LBP, and sCD14 were determined by ELISA from samples collected at weeks 0, 6, and 12. Data were analyzed (SPSS v25.0) for group and time differences using a two-way repeated-measures ANOVA with post hoc analysis. No significant differences were observed for any of the measured plasma biomarkers. The previously observed increase in anti-inflammatory cytokine response is likely due to a muscular cellular response and not an indication of intestinal epithelial integrity disturbance and/or subsequent translocation of luminal originated pathogenic bacterial compounds.


Assuntos
Leite , Treinamento de Força , Idoso , Envelhecimento , Animais , Anti-Inflamatórios , Bebidas , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Síndrome de Resposta Inflamatória Sistêmica
13.
Medicina (B Aires) ; 82 Suppl 3: 2-6, 2022 Aug 30.
Artigo em Espanhol | MEDLINE | ID: mdl-36054849

RESUMO

Autism is a neurodevelopmental disorder characterized by deficits in social cognition and communication, restricted interests, and stereotyped behaviors. It is often associated with sensory dysfunctions, other neurodevelopmental disorders, neuropsychiatric disorders, epilepsy and/or sleep disorders. This condition will accompany people throughout their lives, with variations in its evolution. In the last ten years, only 1% of all autism research focused on developing post-secondary education programs and training in adult health services. Taking into account this dichotomy of interests, we must think about the different stages of life, such as early intervention, school inclusion, bullying, associated mental and medical disorders, lack of group belonging, job opportunities and the aging. Only by becoming aware of each of them can we achieve a better quality of life for people with autism and their families.


El autismo es un trastorno del neurodesarrollo de base neurobiológica caracterizado por déficits en la cognición social y la comunicación, intereses restringidos y conductas estereotipadas. Muchas veces está asociado a disfunciones sensoriales, otros trastornos del neurodesarrollo, trastornos neuropsiquiátricos, epilepsia y/o trastornos de sueño. Esta condición acompañará a las personas a lo largo de toda la vida, con variaciones en su evolución. En los últimos diez años solo el 1% de todas las investigaciones en autismo se centraron en desarrollar programas de educación post secundaria, y formación de servicios de salud para adultos. Teniendo en cuenta esta dicotomía de intereses es que debemos pensar en las diferentes etapas de la vida, cómo el abordaje temprano, la inclusión escolar, el hostigamiento, los trastornos mentales y médicos asociados, la falta de grupo de pertenencia, las oportunidades laborales y el envejecimiento. Solamente tomando conciencia de cada una de ellas podremos lograr una mejor calidad de vida de las personas con autismo y sus familias.


Assuntos
Transtorno Autístico , Epilepsia , Adulto , Envelhecimento , Humanos , Qualidade de Vida , Comportamento Estereotipado
14.
Sci Rep ; 12(1): 15012, 2022 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-36056136

RESUMO

Several factors including sex and lifestyle have been reported to contribute to the age-related alteration of immune functions. The study was undertaken to determine age-related differences in the proportion of peripheral blood mononuclear lymphocytes in the Indian population using blood samples from 67 healthy adults (33 females and 34 males) aged between 20 and 80 years old. In the linear regression analysis to estimate the relationship with age categories, there was a significant increase in the frequency of natural killer cells with ageing, while their cytolytic activity significantly declined. The frequency of CD4+ T cells increased with age, whereas that of CD8+ T cells decreased, resulting in the age-associated increase of the CD4/CD8 ratio. The subsets of B cells did not show any significant relationship with age. Although there were variations between the male and female subgroups in effect size of ageing, the trends were in the same direction in all the parameters. Reduced fat intake was associated with a lower frequency of CD4+ T cells, and higher serum cotinine level was associated with a higher CD4/CD8 ratio. The results indicate that cellular immunity in the Indian population is affected by ageing, while humoral immunity is less susceptible to ageing.


Assuntos
Linfócitos T CD8-Positivos , Leucócitos Mononucleares , Adulto , Idoso , Idoso de 80 Anos ou mais , Envelhecimento , Feminino , Citometria de Fluxo , Humanos , Estilo de Vida , Masculino , Pessoa de Meia-Idade , Adulto Jovem
15.
Front Public Health ; 10: 952619, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36062097

RESUMO

With the global growth of the aging population, healthy aging and active aging has become an important goal for the future social development of all countries. The purpose of this study is to explore the potential relationships between the older people's perceived importance of the Internet, family atmosphere, behavioral independence, life satisfaction, and health. The data come from the China Family Panel Studies' fourth wave (2015-2016) and fifth wave (2017-2018) investigations. According to an analysis of data of 5,948 people over 60 years old performed using LISREL 8.8 software, the selected cases answered the same questions about the perceived importance of the Internet, life satisfaction, and health status in two waves of surveys. The results show that life satisfaction and self-rated health have cross influences, while at the same time both are persistent in the time baseline, and family atmosphere and behavioral independence play an important intermediary role. Therefore, strengthening parent-child interaction, promoting parent-child relationships, and improving behavioral independence can effectively improve the life satisfaction and health status of the older people.


Assuntos
Nível de Saúde , Satisfação Pessoal , Idoso , Envelhecimento , China/epidemiologia , Humanos , Internet , Pessoa de Meia-Idade
16.
Neuroreport ; 33(14): 623-628, 2022 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-36062512

RESUMO

N-methyl-D-aspartate (NMDA) receptor antagonists mediate adult neurogenic effects. Here, the neurogenic effect of a new NMDA receptor antagonist endowed with neuroprotective effects in Alzheimer's disease mice model. Nine-month-old senescence-accelerated mouse prone 8 (SAMP8) with UB-ALT-EV were orally treated. 5-Bromo-2-deoxyuridine (BrdU) (50 mg/kg) was 3× injected I.P. every 2 h. After 28 days of treatment, SAMP8-treated group improved working memory. Moreover, the number of BrdU+ cells and DCX+ cells in the SAMP8 dentate gyrus (DG) was significantly increased. GFAP+ cells were not affected by treatment. Together, these results provided evidence that UB-ALT-EV promotes the survival and proliferation of neural progenitor cells in the aged SAMP8 hippocampus.


Assuntos
Células-Tronco Neurais , Receptores de N-Metil-D-Aspartato , Envelhecimento , Animais , Bromodesoxiuridina , Hipocampo/metabolismo , Camundongos , Células-Tronco Neurais/metabolismo , Neurogênese , Receptores de N-Metil-D-Aspartato/metabolismo
18.
Can J Exp Psychol ; 76(3): 210-217, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-36048080

RESUMO

Intentional forgetting aims to prevent unwanted information from being stored in long-term memory. Surprisingly, past research has shown that, relative to younger adults, older adults recall and recognize more to-be-forgotten information. It has been suggested that this occurs because older adults have a deficient ability to inhibit information. In two experiments, we examined memory differences between older and younger adults in an item-method directed forgetting task. Participants viewed words one at a time during a study phase, each followed by a cue to remember (R) or to forget (F). In Experiment 1, participants' later recognition of both types of items was assessed, followed by a separate source discrimination test for the cue that had been associated with each word at study. In Experiment 2, memory was assessed using a three-response recognition test, indicating whether each word was either new or previously studied and, if previously studied, whether it was associated with an R cue or an F cue. In both experiments, older and younger adults recognized more to-be-remembered items than to-be-forgotten items, the typical directed forgetting effect (DFE). Contrary to past reports, older adults did not remember more to-be-forgotten items than did younger adults, inconsistent with an inhibitory deficit. Older adults were, however, less accurate than younger adults in identifying cue associations for both R and F items, consistent instead with an associative memory deficit. (PsycInfo Database Record (c) 2022 APA, all rights reserved).


Assuntos
Rememoração Mental , Reconhecimento Psicológico , Idoso , Envelhecimento , Sinais (Psicologia) , Humanos , Inibição Psicológica , Rememoração Mental/fisiologia , Reconhecimento Psicológico/fisiologia
19.
Nat Commun ; 13(1): 5128, 2022 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-36050300

RESUMO

The decline of CD8+ T cell functions contributes to deteriorating health with aging, but the mechanisms that underlie this phenomenon are not well understood. We use single-cell RNA sequencing with both cross-sectional and longitudinal samples to assess how human CD8+ T cell heterogeneity and transcriptomes change over nine decades of life. Eleven subpopulations of CD8+ T cells and their dynamic changes with age are identified. Age-related changes in gene expression result from changes in the percentage of cells expressing a given transcript, quantitative changes in the transcript level, or a combination of these two. We develop a machine learning model capable of predicting the age of individual cells based on their transcriptomic features, which are closely associated with their differentiation and mutation burden. Finally, we validate this model in two separate contexts of CD8+ T cell aging: HIV infection and CAR T cell expansion in vivo.


Assuntos
Linfócitos T CD8-Positivos , Infecções por HIV , Envelhecimento/genética , Linfócitos T CD8-Positivos/metabolismo , Estudos Transversais , Infecções por HIV/genética , Infecções por HIV/metabolismo , Humanos , Transcriptoma
20.
Sci Rep ; 12(1): 14863, 2022 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-36050338

RESUMO

Quantifying skin aging changes and characterizing its 3D structure and function in a non-invasive way is still a challenging area of research, constantly evolving with the development of imaging methods and image analysis tools. In vivo multiphoton imaging offers means to assess skin constituents in 3D, however prior skin aging studies mostly focused on 2D analyses of dermal fibers through their signals' intensities or densities. In this work, we designed and implemented multiphoton multiparametric 3D quantification tools for in vivo human skin pigmentation and aging characterization. We first demonstrated that despite the limited field of view of the technic, investigation of 2 regions of interest (ROIs) per zone per volunteer is a good compromise in assessing 3D skin constituents in both epidermis and superficial dermis. We then characterized skin aging on different UV exposed areas-ventral and dorsal forearms, face. The three major facts of aging that are epidermal atrophy, the dermal-epidermal junction (DEJ) flattening and dermal elastosis can be non-invasively quantified and compared. Epidermal morphological changes occur late and were only objectified between extreme age groups. Melanin accumulation in suprabasal layers with age and chronic exposure on ventral and dorsal forearms is less known and appears earlier. Superficial dermal aging changes are mainly elastin density increase, with no obvious change in collagen density, reflected by SHGto2PEF ratio and SAAID index decrease and ImbrN index increase on all skin areas. Analysis of the z-dermal distribution of these parameters highlighted the 2nd 20 µm thickness normalized dermal sub-layer, that follows the DEJ shape, as exhibiting the highest aging differences. Moreover, the 3D ImbrN index allows refining the share of photoaging in global aging on face and the 3D SAAID index on forearm, which elastin or fibrillar collagens densities alone do not allow. Photoaging of the temple area evolves as a function of chronic exposure with a more pronounced increase in elastin density, also structurally modified from thin and straight elastic fibers in young volunteers to dense and compact pattern in older ones. More generally, multiphoton multiparametric 3D skin quantification offers rich spatial information of interest in assessing normal human skin condition and its pathological, external environment or product induced changes.


Assuntos
Envelhecimento da Pele , Dermatopatias , Idoso , Envelhecimento , Elastina/química , Antebraço , Humanos , Pele/diagnóstico por imagem
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