Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 6.105
Filtrar
1.
Medicine (Baltimore) ; 99(1): e18605, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31895811

RESUMO

To investigate the age-related nomograms and change trends of reproductive hormones, and prevalence of androgen deficiency (AD) in middle-aged and aging men from 2 studies.Two cross-sectional studies were conducted at 5-year intervals in Chinese community-dwelling men living in the same area. A total of 434 (Study 1, S1) and 944 (Study 2, S2) men aged 40 to 69 years were recruited as subjects and 59 (S1) and 98 (S2) men aged 20 to 39 years as controls to measure serum reproductive hormone levels.Serum total testosterone (TT) levels did not change significantly in S1, whereas TT levels increased in S2 with aging. Serum calculated free testosterone (cFT) levels gradually decreased with aging; however, only men aged 40 to 69 years showed this trend in S2. Serum luteinizing hormone (LH) and sex hormone binding globulin (SHBG) levels gradually increased, and serum testosterone secretion index (TSI) and free testosterone index (FTI) levels gradually decreased with male aging. The mean annual decrease values of serum cFT were 2.705 pmol/l in S1 and 1.060 pmol/l in S2. The cut-off values for AD in S1 and S2 were 9.13 nmol/l and 9.35 nmol/l for TT, and 169.00 pmol/l and 213.90 pmol/l for cFT. Using TT or cFT cut-off values, mean AD prevalence was 14.52% or 44.70% in S1, and 6.36% or 16.53% in S2. Based on cFT cut-off values, prevalence of AD increased gradually with male aging in a range of 25.30% to 61.63% in S1 and 1.20% to 23.03% in S2.The change patterns of serum LH, SHBG, TSI and FTI levels in middle-aged and aging males were consistent; however, there were differences in serum TT and cFT change patterns in S1 and S2 with male aging. cFT cut-off values were the optimal metric to evaluate AD, which can be present a ladder-like change in prevalence of different age groups.


Assuntos
Envelhecimento/sangue , Doenças do Sistema Endócrino/epidemiologia , Hormônio Luteinizante/sangue , Globulina de Ligação a Hormônio Sexual/metabolismo , Testosterona/sangue , Adulto , Idoso , China/epidemiologia , Estudos Transversais , Humanos , Masculino , Pessoa de Meia-Idade , Nomogramas , Prevalência , Testosterona/deficiência , Adulto Jovem
3.
Nat Med ; 25(12): 1843-1850, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31806903

RESUMO

Aging is a predominant risk factor for several chronic diseases that limit healthspan1. Mechanisms of aging are thus increasingly recognized as potential therapeutic targets. Blood from young mice reverses aspects of aging and disease across multiple tissues2-10, which supports a hypothesis that age-related molecular changes in blood could provide new insights into age-related disease biology. We measured 2,925 plasma proteins from 4,263 young adults to nonagenarians (18-95 years old) and developed a new bioinformatics approach that uncovered marked non-linear alterations in the human plasma proteome with age. Waves of changes in the proteome in the fourth, seventh and eighth decades of life reflected distinct biological pathways and revealed differential associations with the genome and proteome of age-related diseases and phenotypic traits. This new approach to the study of aging led to the identification of unexpected signatures and pathways that might offer potential targets for age-related diseases.


Assuntos
Envelhecimento/sangue , Proteínas Sanguíneas/genética , Longevidade/genética , Proteoma/genética , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Envelhecimento/genética , Animais , Doença Crônica , Feminino , Humanos , Masculino , Camundongos , Pessoa de Meia-Idade , Fatores de Risco , Adulto Jovem
4.
BMJ ; 367: l6055, 2019 11 20.
Artigo em Inglês | MEDLINE | ID: mdl-31748235

RESUMO

OBJECTIVE: To determine the relation between age and troponin level and its prognostic implication. DESIGN: Retrospective cohort study. SETTING: Five cardiovascular centres in the UK National Institute for Health Research Health Informatics Collaborative (UK-NIHR HIC). PARTICIPANTS: 257 948 consecutive patients undergoing troponin testing for any clinical reason between 2010 and 2017. MAIN OUTCOME MEASURE: All cause mortality. RESULTS: 257 948 patients had troponin measured during the study period. Analyses on troponin were performed using the peak troponin level, which was the highest troponin level measured during the patient's hospital stay. Troponin levels were standardised as a multiple of each laboratory's 99th centile of the upper limit of normal (ULN). During a median follow-up of 1198 days (interquartile range 514-1866 days), 55 850 (21.7%) deaths occurred. A positive troponin result (that is, higher than the upper limit of normal) signified a 3.2 higher mortality hazard (95% confidence interval 3.1 to 3.2) over three years. Mortality varied noticeably with age, with a hazard ratio of 10.6 (8.5 to 13.3) in 18-29 year olds and 1.5 (1.4 to 1.6) in those older than 90. A positive troponin result was associated with an approximately 15 percentage points higher absolute three year mortality across all age groups. The excess mortality with a positive troponin result was heavily concentrated in the first few weeks. Results were analysed using multivariable adjusted restricted cubic spline Cox regression. A direct relation was seen between troponin level and mortality in patients without acute coronary syndrome (ACS, n=120 049), whereas an inverted U shaped relation was found in patients with ACS (n=14 468), with a paradoxical decline in mortality at peak troponin levels >70×ULN. In the group with ACS, the inverted U shaped relation persisted after multivariable adjustment in those who were managed invasively; however, a direct positive relation was found between troponin level and mortality in patients managed non-invasively. CONCLUSIONS: A positive troponin result was associated with a clinically important increased mortality, regardless of age, even if the level was only slightly above normal. The excess mortality with a raised troponin was heavily concentrated in the first few weeks. STUDY REGISTRATION: ClinicalTrials.gov NCT03507309.


Assuntos
Envelhecimento/sangue , Doenças Cardiovasculares , Troponina/sangue , Adulto , Idoso , Idoso de 80 Anos ou mais , Biomarcadores/sangue , Doenças Cardiovasculares/sangue , Doenças Cardiovasculares/mortalidade , Doenças Cardiovasculares/terapia , Estudos de Coortes , Tratamento Conservador/estatística & dados numéricos , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Mortalidade , Valor Preditivo dos Testes , Prognóstico , Modelos de Riscos Proporcionais , Fatores de Risco , Reino Unido/epidemiologia
5.
Prague Med Rep ; 120(2-3): 84-94, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31586507

RESUMO

Ageing is associated with the accumulation of damage to all the macromolecules within and outside cells leading to progressively more cellular and tissue defects and resulting in age-related frailty, disability and disease. As a result of the aging process the bone deteriorates in composition, structure and function. Age-related musculoskeletal losses are a major public health burden because they can cause physical disability and increased mortality. We tried to find out on a small set of old women, without risk factors for osteoporosis, what caused them the loss of bone minerals. All 492 women had just only one risk factor - the old age. Laboratory findings have shown a decreased serum C telopeptide and low serum alkaline phosphatase circulating markers, used to quantify bone resorption and formation, and very low level of vitamin D. Very low level of vitamin D that disrupted calcium absorption through the intestine, and decreased calcemia increased parathyroid hormone levels with resulting bone effect. The manifestation of physiological aging is worsening eyesight, peripheral neuropathy, depression, worsening of physical condition, skin aging, sarcopenia and bone mineral loss. Senile osteoporosis, which is not caused by known risk factors for osteoporosis, does not appear to be a separate disease, but is part of the physiological process of aging. Treatment of senile osteoporosis should be focused on the control of secondary hyperparathyroidism by administration of vitamin D and calcium. The risk of fractures in the advanced age is determined by a large number of factors ranging from hazards in the home environment to frailty and poor balance.


Assuntos
Envelhecimento/sangue , Envelhecimento/patologia , Fosfatase Alcalina/sangue , Densidade Óssea , Cálcio/metabolismo , Colágeno Tipo I/sangue , Feminino , Humanos , Osteogênese , Osteoporose/sangue , Hormônio Paratireóideo/sangue , Peptídeos/sangue , Vitamina D/sangue
6.
Ann Agric Environ Med ; 26(3): 489-495, 2019 Sep 19.
Artigo em Inglês | MEDLINE | ID: mdl-31559809

RESUMO

INTRODUCTION AND OBJECTIVE: Deficits of vitamin resources constitute a significant public health problem, especially among the elderly population. The aim of the research was to determine the level of vitamin 25 (OH) D and vitamins from group B in the chronically ill elderly in domiciliary care, depending on functional capacity and coexisting diseases. MATERIAL AND METHODS: The pilot study included 137 patients staying in long-term domiciliary care. Samples of the participants' venous blood was obtained for laboratory tests. Centrifuged serum was used to determine the level of the following biochemical parameters: vitamin 25 (OH)D, B12, folic acid and total protein, albumin, triglycerides, total cholesterol and HDL cholesterol. Assessment of the functional status of patients was made by using the Barthel scale. RESULTS: More than ¾ of the patients with functional deficit (according to Barthel's score 0-85 points) were deficient in vitamin 25 (OH)D, while folic acid values were below the reference values in more than half of the patients. Respondents with lower functional efficiency were characterised by a reduced average value of vitamin 25 (OH)D and folic acid. CONCLUSIONS: The studied group of the chronically ill elderly was characterised by a deficiency of vitamin D3 and folic acid. Subjects with a functional impairment deficit show a reduced mean value of vitamin 25 (OH)D and folic acid in the blood serum, compared to the group of patients with higher mobility.


Assuntos
Envelhecimento/sangue , Doença Crônica/terapia , Vitamina B 12/sangue , Vitamina D/sangue , Idoso , Idoso de 80 Anos ou mais , Envelhecimento/fisiologia , Feminino , Ácido Fólico/sangue , Serviços de Assistência Domiciliar/estatística & dados numéricos , Humanos , Locomoção , Masculino , Atividade Motora , Projetos Piloto , Triglicerídeos/sangue
7.
Artigo em Inglês | MEDLINE | ID: mdl-31509977

RESUMO

A low serum high-density lipoproteins-cholesterol (HDL-C) level is a risk factor of cardiovascular disease and dementia. On the other hand, no study has elucidated the correlation between household income and the HDL-C level in the adult population. In the present study, 5535 subjects (20-80 year-old individuals) were selected from the Korean national health and nutrition examination survey 2017 (KNHANES VII-2, n = 2469 men, n = 3066 women). They were classified into five levels of household income grades ranging from one (the lowest) to five (the highest). They were also classified according to the HDL-C level: category 1 (<40 mg/dL, n = 943), category 2 (40-49 mg/dL, n = 1764), category 3 (50-59 mg/dL, n = 1572), category 4 (60-69 mg/dL, n = 820), and category 5 (≥70 mg/dL, n = 436). Generally, in both genders, a higher HDL-C level is associated with a larger percentage of income grades 4 and 5. Moreover, the lowest HDL-C group showed the largest percentage of income grade 1. In both groups, a significant increase in the average income grade was associated with a concomitant increase in the HDL-C level (men, p = 0.03, women, p < 0.001). In the low HDL-C category, a lower income grade is associated directly with a lower HDL-C level, which suggests that poverty is associated directly with a low HDL-C. Women showed a 3.3-fold higher incidence of dementia than men did at later-life. The sharp decrease in HDL-C in the female group older than 50 was accompanied by a dramatic increase in the incidence of dementia. However, the male group showed a relatively mild decrease in the HDL-C level after mid-life and weak elevation in the incidence of dementia. In conclusion, in both genders, the lower income group showed a larger prevalence of low-HDL-C levels. The decrease in HDL-C after middle age was strongly associated with the considerable increase in dementia in later-life.


Assuntos
Envelhecimento/sangue , HDL-Colesterol/sangue , Pobreza , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Renda , Masculino , Pessoa de Meia-Idade , Inquéritos Nutricionais , República da Coreia/epidemiologia , Adulto Jovem
8.
Biol Pharm Bull ; 42(8): 1423-1427, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31366878

RESUMO

Age is known as one of influencing factor for theophylline (TP)-metabolizing capacity. In a previous our study, the ratio of TP and its major metabolite 1,3-dimethyluric acid (DMU) in serum (DMU/TP) is a useful index to estimate TP-metabolizing capacity, and this value markedly increased by influencing factor, such as the history of smoking. However, it is unknown whether DMU/TP values in serum reflect age-associated changes of TP-metabolizing capacity. In this study, the effect of age on the DMU/TP values in serum were investigated using mice of different age due to the limited blood sampling in human. The concentrations of TP and its metabolites in mouse serum were simultaneously measured using HPLC. As observed in human serum, serum TP concentrations were closely correlated with DMU concentration in mice, which indicates that the DMU/TP ratio is a good indicator of TP metabolic ability in mice. When TP was administered subcutaneously in 2-28-week-old mice, age-associated changes in the DMU/TP ratio in mice were observed. In conclusion, age-associated changes in TP-metabolizing capacity can be estimated by the DMU/TP ratio in serum.


Assuntos
Envelhecimento/sangue , Teofilina/sangue , Ácido Úrico/análogos & derivados , Envelhecimento/metabolismo , Animais , Masculino , Camundongos Endogâmicos ICR , Teofilina/farmacocinética , Ácido Úrico/sangue
9.
Biol Sex Differ ; 10(1): 34, 2019 07 08.
Artigo em Inglês | MEDLINE | ID: mdl-31287027

RESUMO

BACKGROUND: Chronic inflammation and impaired sleep increase the risk for cardiovascular disease. Menopausal women may be particularly at risk as a result of impaired sleep. The objective of the current investigation was to assess the relationship between poor sleep and C-reactive protein (CRP), interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-α), and myeloperoxidase (MPO) in healthy non- and postmenopausal women and men. METHODS: A fasting blood draw was obtained from 122 healthy men and women (31 were postmenopausal). Higher scores on the Pittsburgh Sleep Quality Index (PSQI) were used to define poor sleep. Given the sample size and healthy nature of the sample, hierarchical linear regression analyses were performed on a composite inflammatory score involving CRP, IL-6, and TNF-α. Sex/menopausal group and PSQI were entered as predictors, and the interaction of the group by PSQI was entered stepwise. Analyses on MPO were performed separately. RESULTS: Sleep quality was associated with higher inflammatory activity (ß = 0.272, P = 0.003), which remained significant (P = 0.046) after controlling for age, waist circumference, exercise times per week, and depressive symptoms. While in the same direction, sleep quality was not significantly associated with MPO. Dichotomizing sleep quality led to similar results. CONCLUSION: Impaired sleep quality is independently associated with greater inflammation in healthy adult men and women. Despite an overall less favorable metabolic and inflammatory profile in postmenopausal women, impaired sleep did not emerge as differentially related to inflammatory activity in this group.


Assuntos
Proteína C-Reativa/análise , Interleucina-6/sangue , Peroxidase/sangue , Pós-Menopausa/sangue , Transtornos do Sono-Vigília/sangue , Fator de Necrose Tumoral alfa/sangue , Adulto , Envelhecimento/sangue , Biomarcadores/sangue , Feminino , Humanos , Inflamação/sangue , Masculino , Pessoa de Meia-Idade , Pré-Menopausa/sangue
10.
Drugs Aging ; 36(8): 747-758, 2019 08.
Artigo em Inglês | MEDLINE | ID: mdl-31161580

RESUMO

BACKGROUND: Age-related changes in the concentration-effect relationship of (+)-O-desmethyl-tramadol [(+)-ODM], tramadol's active metabolite, are not documented in the elderly. OBJECTIVE: The objective of this study was to characterize, in elderly and young subjects, the (+)-ODM pharmacokinetic and pharmacodynamic relationship to examine the effect of age after single-dose administration of tramadol 200 mg extended-release tablets. METHODS: A population analysis of a double-blind, randomized, placebo-controlled, two-period cross-over study including 13 elderly (aged ≥75 years) subjects with mild renal insufficiency and 16 young (aged 18-40 years) subjects was conducted. For 48 h post-dose, blood samples were collected and pain tolerance thresholds measured using an electrically stimulated pain model. A pharmacokinetic/pharmacodynamic model incorporating a one-compartment pharmacokinetic model for (+)-ODM parameterized with first-order formation rate, clearance (CL/fm), volume of distribution (V/fm) and a sigmoid maximum effect (Emax) model incorporating baseline (E0) and placebo effect was used. RESULTS: Maximum plasma concentrations of (+)-ODM occurred later and plasma concentrations declined more slowly in the elderly than in young subjects. In the elderly, V/fm was 76% larger and CL/fm 16% slower. Baseline (E0) and sensitivity (C50) for pain tolerance were similar between young and elderly subjects. However, the Emax parameter was 2.5 times higher in the elderly and maximum possible treatment-related effect was 169 (135-221) in the young and 194 (149-252) in the elderly; that is, 15% higher in the elderly. CONCLUSIONS: This exploratory analysis suggests that age-related differences exist in the distribution and elimination of (+)-ODM, including a 76% larger distribution outside the central compartment and 16% slower clearance of (+)-ODM. These pharmacokinetic changes are associated with a 15% higher maximum possible treatment-related effect and carry the potential for greater efficacy but also the potential for increased side effects at the same dose in elderly subjects. Clinicaltrials.gov identifier: NCT02329561.


Assuntos
Envelhecimento/sangue , Analgésicos Opioides/sangue , Analgésicos Opioides/farmacologia , Modelos Biológicos , Dor/tratamento farmacológico , Tramadol/análogos & derivados , Adolescente , Adulto , Idoso , Analgésicos Opioides/administração & dosagem , Estudos Cross-Over , Método Duplo-Cego , Estimulação Elétrica , Feminino , Voluntários Saudáveis , Humanos , Masculino , Pessoa de Meia-Idade , Dor/sangue , Tramadol/administração & dosagem , Tramadol/sangue , Tramadol/farmacologia , Adulto Jovem
11.
Nutrients ; 11(6)2019 May 30.
Artigo em Inglês | MEDLINE | ID: mdl-31151228

RESUMO

Time-restricted feeding (TRF) is a form of intermittent fasting that involves having a longer daily fasting period. Preliminary studies report that TRF improves cardiometabolic health in rodents and humans. Here, we performed the first study to determine how TRF affects gene expression, circulating hormones, and diurnal patterns in cardiometabolic risk factors in humans. Eleven overweight adults participated in a 4-day randomized crossover study where they ate between 8 am and 2 pm (early TRF (eTRF)) and between 8 am and 8 pm (control schedule). Participants underwent continuous glucose monitoring, and blood was drawn to assess cardiometabolic risk factors, hormones, and gene expression in whole blood cells. Relative to the control schedule, eTRF decreased mean 24-hour glucose levels by 4 ± 1 mg/dl (p = 0.0003) and glycemic excursions by 12 ± 3 mg/dl (p = 0.001). In the morning before breakfast, eTRF increased ketones, cholesterol, and the expression of the stress response and aging gene SIRT1 and the autophagy gene LC3A (all p < 0.04), while in the evening, it tended to increase brain-derived neurotropic factor (BNDF; p = 0.10) and also increased the expression of MTOR (p = 0.007), a major nutrient-sensing protein that regulates cell growth. eTRF also altered the diurnal patterns in cortisol and the expression of several circadian clock genes (p < 0.05). eTRF improves 24-hour glucose levels, alters lipid metabolism and circadian clock gene expression, and may also increase autophagy and have anti-aging effects in humans.


Assuntos
Envelhecimento/sangue , Glicemia , Ritmo Circadiano/fisiologia , Ingestão de Alimentos , Envelhecimento/fisiologia , Autofagia , Biomarcadores/sangue , Estudos Cross-Over , Jejum , Regulação da Expressão Gênica , Humanos , Refeições , Fatores de Tempo
12.
J Agric Food Chem ; 67(28): 7832-7843, 2019 Jul 17.
Artigo em Inglês | MEDLINE | ID: mdl-31242723

RESUMO

Oxidative-stress-induced senescence constitutes a great risk factor for chronic diseases. Therefore, ameliorating oxidative-stress-induced senescence is expected to prevent chronic diseases. The beneficial effects of bilberry anthocyanin (BA) on healthy aging were evaluated using 12 month old, aging female SD rats in this study. The experimental results suggested that consumption of a middle-dose of BA (MBA) appreciably increased the relative liver mass by 7.34% when compared with that of the AC group. Furthermore, BA significantly increased the total antioxidant capacity, total superoxide dismutase activity, and catalase activities; decreased malondialdehyde, serum low-density lipoprotein cholesterol (LDL-C), serum total cholesterol (TC), serum triglyceride (TG), and glycated serum protein (GSP) levels; and reduced TC/high-density lipoprotein cholesterol (HDL-C) and LDL-C/HDL-C ratios. In addition, MBA decreased the activity of fecal bacterial enzymes and increased the content of fecal short-chain fatty acids. The Western blot results showed that MBA significantly upregulated the expression of OCLN, ZO-1, and autophagy-related proteins (ATP6 V0C, ATG4D, and CTSB) in aging rats. Moreover, it also showed that MBA induced the phosphorylation of AMPK and FOXO3a and inhibited the phosphorylation of mTOR, which indicated that bilberry anthocyanin induced autophagy via the AMPK-mTOR signaling pathways. This induction of autophagy further promoted oxidative stress resistance effects and intestinal epithelial barrier function of bilberry anthocyanin in aging female rats.


Assuntos
Proteínas Quinases Ativadas por AMP/metabolismo , Envelhecimento/fisiologia , Antocianinas/administração & dosagem , Autofagia/efeitos dos fármacos , Serina-Treonina Quinases TOR/metabolismo , Vaccinium myrtillus/química , Proteínas Quinases Ativadas por AMP/genética , Envelhecimento/sangue , Envelhecimento/efeitos dos fármacos , Envelhecimento/genética , Animais , Suplementos Nutricionais/análise , Feminino , Proteína Forkhead Box O3/genética , Proteína Forkhead Box O3/metabolismo , Humanos , Lipoproteínas LDL/sangue , Malondialdeído/sangue , Estresse Oxidativo/efeitos dos fármacos , Ratos , Ratos Sprague-Dawley , Transdução de Sinais/efeitos dos fármacos , Serina-Treonina Quinases TOR/genética , Triglicerídeos/sangue
13.
J Steroid Biochem Mol Biol ; 193: 105409, 2019 10.
Artigo em Inglês | MEDLINE | ID: mdl-31201927

RESUMO

New reference intervals need to be established for a new analytical method with improved sensitivity and specificity. We aimed to establish the new reference intervals from infancy to senescence of nine steroid hormones (cortisol, cortisone, progesterone, 17-hydroxyprogesterone (17-OHP), androstenedione, testosterone, estradiol, DHEAS, and aldosterone) for LC-MS/MS method. Serum samples from 4678 reference individuals (age range: 0.3-79 years) were measured with LC-MS/MS. Samples were collected between 7 a.m. and 10 a.m. Exclusion criteria were concomitant endocrine diseases and body mass index ≥ 33. Generalized additive model for location, scale and shape, the nonparametric or robust method was applied. We established the reference intervals of the nine steroid hormones by sex, age, and pubertal stage. Below the age of one, we observed the surge of androgen and estrogen which implied mini-puberty. At the same period of life, aldosterone and cortisone levels were very high reflecting physiological hyperaldosteronism. An increase of steroid hormones during the pubertal development and slow decrease towards senescence after the peak at early adulthood were observed. Due to the increase of CBG synthesis, cortisol levels were increased under oral contraceptives (OC) significantly (p < 0.0001), while OC suppressed progesterone, 17-OHP, androstenedione, and estradiol (p < 0.0001). Our results will facilitate the interpretation of patient data in routine diagnostics with the use of LC-MS/MS method. Since LC-MS/MS methods have shown good comparability among the different laboratories, our reference intervals can be further adopted in other laboratories equipped with LC-MS/MS, once the validation with a small number of reference samples is performed.


Assuntos
Envelhecimento/sangue , Caracteres Sexuais , Esteroides/sangue , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Pré-Escolar , Cromatografia Líquida , Anticoncepcionais Orais/uso terapêutico , Feminino , Humanos , Lactente , Longevidade , Masculino , Pessoa de Meia-Idade , Puberdade/metabolismo , Valores de Referência , Espectrometria de Massas em Tandem , Adulto Jovem
14.
Vopr Pitan ; 88(2): 58-63, 2019.
Artigo em Russo | MEDLINE | ID: mdl-31233689

RESUMO

The high incidence of chronic pancreatitis (CP) which arise at young people of working age, the development of serious complications are pushing for the development of new diagnostic methods and the search for effective ways to treat this disease. In this regard, a relevant and promising direction is the further study of the mechanisms of pathogenesis and the formation of trophological (including mineral) deficiency in CP, followed by the development of comprehensive programs for their correction. Aim is to study mineral status of patients with chronic pancreatitis, depending on their age. Material and methods. A sample of 218 patients (140 women, 78 men) with CP with exocrine insufficiency aged 18 to 72 years was examined. The control group consisted of 20 healthy individuals who underwent a planned outpatient examination. To assess the mineral status, macronutrients were determined photometrically, iron by the bathophenanthroline method, the remaining trace elements (Cu, Zn, Pb, Cd) were determined by atomic absorption spectrometry in blood serum. Results and discussion. Patients with CP at all age groups revealed a statistically significant (p<0.001) decrease in serum concentration of calcium, phosphorus, magnesium, potassium, copper, zinc, and iron compared to the level in healthy individuals, which increased with age. In the group of patients older than 60 years, the state of hypomineralemia was detected by the level of calcium, phosphorus, magnesium, potassium, copper, zinc, iron, which required correction of the mineral status. With increasing age of patients with CP, the content of toxic metals (lead and cadmium) increased compared with that in the group of healthy individuals. Сonclusions. The findings suggest that the age of patients with CP is a predictor of mineral deficiency and the accumulation of toxic elements, which must be considered when forming a complex treatment.


Assuntos
Envelhecimento/sangue , Minerais/sangue , Pancreatite Crônica/sangue , Oligoelementos/sangue , Adolescente , Adulto , Fatores Etários , Idoso , Envelhecimento/patologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Pancreatite Crônica/patologia
15.
Nat Med ; 25(6): 988-1000, 2019 06.
Artigo em Inglês | MEDLINE | ID: mdl-31086348

RESUMO

An aged circulatory environment can activate microglia, reduce neural precursor cell activity and impair cognition in mice. We hypothesized that brain endothelial cells (BECs) mediate at least some of these effects. We observe that BECs in the aged mouse hippocampus express an inflammatory transcriptional profile with focal upregulation of vascular cell adhesion molecule 1 (VCAM1), a protein that facilitates vascular-immune cell interactions. Concomitantly, levels of the shed, soluble form of VCAM1 are prominently increased in the plasma of aged humans and mice, and their plasma is sufficient to increase VCAM1 expression in cultured BECs and the hippocampi of young mice. Systemic administration of anti-VCAM1 antibody or genetic ablation of Vcam1 in BECs counteracts the detrimental effects of plasma from aged individuals on young brains and reverses aging aspects, including microglial reactivity and cognitive deficits, in the brains of aged mice. Together, these findings establish brain endothelial VCAM1 at the blood-brain barrier as a possible target to treat age-related neurodegeneration.


Assuntos
Envelhecimento/sangue , Encéfalo/metabolismo , Células-Tronco Neurais/metabolismo , Molécula 1 de Adesão de Célula Vascular/metabolismo , Adolescente , Adulto , Idoso , Envelhecimento/imunologia , Envelhecimento/metabolismo , Animais , Barreira Hematoencefálica/imunologia , Barreira Hematoencefálica/metabolismo , Encéfalo/citologia , Células Cultivadas , Células Endoteliais/metabolismo , Feminino , Deleção de Genes , Hipocampo/citologia , Hipocampo/metabolismo , Humanos , Mediadores da Inflamação/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos NOD , Camundongos Knockout , Camundongos SCID , Microglia/metabolismo , Células-Tronco Neurais/citologia , Molécula 1 de Adesão de Célula Vascular/sangue , Molécula 1 de Adesão de Célula Vascular/genética , Adulto Jovem
16.
EBioMedicine ; 44: 28-40, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31130473

RESUMO

BACKGROUND: Aging is a complex physiological phenomenon, intricately associated with cardiovascular pathologies, where platelets play a central pathophysiological role. Although antiplatelets are commonly employed to prevent and treat major adverse cardiovascular events, aging associated intraplatelet changes remain largely unexplored. METHODS: Platelets were studied in high cardiovascular risk patients (aged 40-100 years) comparing them to younger healthy subjects. This was followed by cross sectional and longitudinal mice studies. Flow cytometry, biochemical and molecular assays were used to study platelets comprehensively. FINDINGS: CVD Patients were categorized in the age groups 40-59, 60-79, and 80-100 years. Progressive decline in platelet health was observed in the 40-79 years age cohort, marked by increase in oxidative stress, hyperactivation and apoptotic markers. Paradoxically, this was reversed in patients aged above 79 years and the improved platelet phenotype was associated with lower oxidative damage. The platelets from the very old (80-100 year) group were found to be preloaded with increased antioxidants, which also contributed to higher resistance against induced redox insults. Cross sectional mouse studies excluded the effect of comorbidities and medications. Longitudinal mouse studies implicate an adaptive increase in antioxidant levels as the mechanism. INTERPRETATION: We report a novel age associated, non-linear redox regulation in platelets in both humans and mice. In advanced age, there occurs an adaptive increase in platelet antioxidants, reducing the intracellular ROS and leading to a healthier platelet phenotype. Clinically, our results advocate the use of less aggressive antiplatelet therapies for CVD in the elderly population. FUND: Study funded by NIH-NHLBI, RO1-HL122815 and RO1-HL115247.


Assuntos
Envelhecimento/metabolismo , Plaquetas/metabolismo , Oxirredução , Estresse Oxidativo , Adaptação Fisiológica , Fatores Etários , Idoso , Idoso de 80 Anos ou mais , Envelhecimento/sangue , Animais , Antioxidantes/metabolismo , Apoptose , Biomarcadores , Doenças Cardiovasculares/epidemiologia , Doenças Cardiovasculares/etiologia , Doenças Cardiovasculares/metabolismo , Comorbidade , Modelos Animais de Doenças , Feminino , Homeostase , Humanos , Masculino , Camundongos , Pessoa de Meia-Idade , Ativação Plaquetária , Adesividade Plaquetária , Espécies Reativas de Oxigênio/metabolismo , Medição de Risco , Fatores de Risco
17.
Environ Pollut ; 252(Pt A): 21-30, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31146235

RESUMO

Concentrations of mercury (Hg) were examined in the blood of mute swans from rural breeding sites and urban wintering areas in southern parts of Poland, Europe. The birds were classified into three age groups: cygnets, juveniles and adults. To investigate the potential impact of Hg on birds, hematocrit (Ht), reduced glutathione (GSH) levels and morphometric measurements were taken. Using morphometric parameters, we stated that all mute swans sampled were in good condition. The mercury concentrations found were rather low and differed between birds from industrialized wintering areas and rural breeding areas (means 7 ng/mL and 2 ng/mL, respectively). We found no difference in Hg concentrations between the sexes, but concentrations varied significantly between age groups (cygnets 2 ng/mL, juveniles 7 ng/mL and adults 6 ng/mL). A similar trend was observed for hematocrit levels. GSH levels did not differ between any of the groups studied. We found no significant relationship between blood parameters (Ht, GSH) in relation to Hg concentrations. We conclude that the Hg concentrations in blood may be influenced by industrialization, season and age, but generally low concentration such as those found by us do not affect Ht and GSH levels.


Assuntos
Envelhecimento/sangue , Anseriformes/sangue , Mercúrio/sangue , Animais , Aves , Feminino , Glutationa/sangue , Masculino , Polônia , Estações do Ano
18.
Chemosphere ; 230: 519-526, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31125880

RESUMO

Tebuconazole is a triazole compound used agriculturally to treat plant pathogenic fungi. However, whether pubertal exposure to tebuconazole affects Leydig cell development remains unknown. Here, we exposed male Sprague-Dawley rats at 35 days of age to 0, 25, 50, or 100 mg kg-1 day-1 tebuconazole for 21 days. Tebuconazole exposure increased serum testosterone level but lowered estradiol level at a dose of 100 mg kg-1, without affecting serum luteinizing hormone and follicle-stimulating hormone concentrations. Tebuconazole up-regulated the expression of testicular Cyp11a1, Hsd11b1, and Fshr genes as well as their proteins at a dose of 100 mg kg-1. However, tebuconazole did not stimulate the proliferation of Leydig cells. Tebuconazole in vitro inhibits aromatase activity in primary rat Leydig cells with IC50 value of 40 µmol/L. In conclusion, tebuconazole exposure stimulates pubertal Leydig cell differentiation via inhibiting aromatase activity.


Assuntos
Aromatase/metabolismo , Fungicidas Industriais/toxicidade , Testículo/efeitos dos fármacos , Testosterona/sangue , Triazóis/toxicidade , Envelhecimento/sangue , Animais , Diferenciação Celular/efeitos dos fármacos , Hormônio Foliculoestimulante/sangue , Expressão Gênica/efeitos dos fármacos , Células Intersticiais do Testículo/efeitos dos fármacos , Células Intersticiais do Testículo/enzimologia , Hormônio Luteinizante/sangue , Masculino , Ratos , Ratos Sprague-Dawley , Testículo/enzimologia , Testículo/crescimento & desenvolvimento , Regulação para Cima
19.
Lipids ; 54(5): 321-328, 2019 05.
Artigo em Inglês | MEDLINE | ID: mdl-31087416

RESUMO

While there is considerable evidence supporting health benefits of consuming diets high in omega-3 (n-3) fatty acids, there is no quick and effective tool to measure n-3 intake. The objective of this study was to evaluate the accuracy of a rapid assessment questionnaire (the Omega-3 Checklist) used to quantify intake of n-3 fatty acids. This was done by comparing n-3 intakes to blood biomarkers of n-3 exposure in a population of healthy men and women. In addition, a separate analysis was run including covariates age, sex, and weight, which have been shown to affect n-3 biomarker levels. Reported intake of eicosapentaenoic acid (EPA), docoshexaenoic acid (DHA), and EPA + DHA was correlated with erythrocyte EPA (Spearman's rank correlation rs = 0.51, p < 0.001), DHA (rs = 0.54, p < 0.001), and the Omega-3 Index (rs = 0.57, p < 0.001). These associations remained significant when controlling for age, sex, and weight. Therefore, the Omega-3 Checklist can be a useful, rapid assessment tool to estimate individuals' EPA and DHA intake.


Assuntos
Inquéritos sobre Dietas/normas , Dieta , Ácidos Graxos Ômega-3/administração & dosagem , Adulto , Envelhecimento/sangue , Biomarcadores/sangue , Peso Corporal , Lista de Checagem , Eritrócitos/química , Ácidos Graxos Ômega-3/sangue , Feminino , Humanos , Masculino , Caracteres Sexuais
20.
Maturitas ; 123: 25-31, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-31027673

RESUMO

OBJECTIVES: To determine the association between physical activity and physical fitness levels (i.e. cardiorespiratory fitness and muscular strength) and the shed form of the α-Klotho gene (S-Klotho plasma levels) in middle-aged sedentary adults. STUDY DESIGN AND MAIN OUTCOME MEASURES: A total of 74 middle-aged sedentary adults (52.7% women; 53.7 ± 5.1 years old) were enrolled in the FIT-AGEING study. Physical activity and sedentary time were assessed with a wrist-worn accelerometer. Maximal oxygen uptake (VO2max) was determined by a maximum treadmill test using indirect calorimetry. Lower- and upper-body muscular strength were assessed by an isokinetic strength test and by the hand grip strength test, respectively. The S-Klotho plasma levels were measured in the EDTA plasma using a solid-phase sandwich enzyme-linked immunosorbent assay. RESULTS: Based on the principal-component analysis, overall physical activity, moderate and vigorous physical activity levels, and sedentary time (as outcomes included in the 'sedentary time and physical activity' category) explained a total of 17.5% of the cumulative variance in S-Klotho plasma levels, while extension peak torque, hand grip strength, and maximal oxygen uptake (as outcomes included in the 'physical fitness' category) explained a total of 15.5% of the cumulative variance in S-Klotho plasma levels. Based on the loading of variables in these 2 categories, the percentage of the cumulative variance in S-Klotho plasma level explained was 28.9%s; this reached 33.0% of cumulative variance when sex was included in the model. CONCLUSIONS: In summary, our results indicate that physical activity and physical fitness levels are associated with S-Klotho plasma levels in middle-aged sedentary adults. Therefore, the S-Klotho protein could be a key factor in the relationship between physical activity and physical fitness and health improvements during the ageing process.


Assuntos
Envelhecimento/sangue , Aptidão Cardiorrespiratória , Exercício , Glucuronidase/sangue , Comportamento Sedentário , Adulto , Calorimetria Indireta , Estudos Transversais , Teste de Esforço , Feminino , Força da Mão , Humanos , Masculino , Pessoa de Meia-Idade , Força Muscular , Aptidão Física , Análise de Componente Principal
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA