Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 2.109
Filtrar
1.
Nat Commun ; 12(1): 4880, 2021 08 12.
Artigo em Inglês | MEDLINE | ID: mdl-34385444

RESUMO

Accurate and imperceptible monitoring of electrophysiological signals is of primary importance for wearable healthcare. Stiff and bulky pregelled electrodes are now commonly used in clinical diagnosis, causing severe discomfort to users for long-time using as well as artifact signals in motion. Here, we report a ~100 nm ultra-thin dry epidermal electrode that is able to conformably adhere to skin and accurately measure electrophysiological signals. It showed low sheet resistance (~24 Ω/sq, 4142 S/cm), high transparency, and mechano-electrical stability. The enhanced optoelectronic performance was due to the synergistic effect between graphene and poly (3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS), which induced a high degree of molecular ordering on PEDOT and charge transfer on graphene by strong π-π interaction. Together with ultra-thin nature, this dry epidermal electrode is able to accurately monitor electrophysiological signals such as facial skin and brain activity with low-motion artifact, enabling human-machine interfacing and long-time mental/physical health monitoring.


Assuntos
Eletrodos , Eletrofisiologia/métodos , Epiderme/fisiologia , Desenho de Equipamento/métodos , Monitorização Fisiológica/métodos , Dispositivos Eletrônicos Vestíveis , Artefatos , Compostos Bicíclicos Heterocíclicos com Pontes/química , Condutividade Elétrica , Eletrofisiologia/instrumentação , Eletrofisiologia/normas , Desenho de Equipamento/normas , Grafite/química , Humanos , Estrutura Molecular , Monitorização Fisiológica/instrumentação , Monitorização Fisiológica/normas , Movimento (Física) , Polímeros/química , Poliestirenos/química , Pele
2.
Int J Mol Sci ; 22(16)2021 Aug 11.
Artigo em Inglês | MEDLINE | ID: mdl-34445339

RESUMO

Both agonist studies and loss-of-function models indicate that PPARγ plays an important role in cutaneous biology. Since PPARγ has a high level of basal activity, we hypothesized that epidermal PPARγ would regulate normal homeostatic processes within the epidermis. In this current study, we performed mRNA sequencing and differential expression analysis of epidermal scrapings from knockout mice and wildtype littermates. Pparg-/-epi mice exhibited a 1.5-fold or greater change in the expression of 11.8% of 14,482 identified transcripts. Up-regulated transcripts included those for a large number of cytokines/chemokines and their receptors, as well as genes associated with inflammasome activation and keratinization. Several of the most dramatically up-regulated pro-inflammatory genes in Pparg-/-epi mouse skin included Igfl3, 2610528A11Rik, and Il1f6. RT-PCR was performed from RNA obtained from non-lesional full-thickness skin and verified a marked increase in these transcripts, as well as transcripts for Igflr1, which encodes the receptor for Igfl3, and the 2610528A11Rik receptor (Gpr15). Transcripts for Il4 were detected in Pparg-/-epi mouse skin, but transcripts for Il17 and Il22 were not detected. Down-regulated transcripts included sebaceous gland markers and a number of genes associated with lipid barrier formation. The change in these transcripts correlates with an asebia phenotype, increased transepidermal water loss, alopecia, dandruff, and the appearance of spontaneous inflammatory skin lesions. Histologically, non-lesional skin showed hyperkeratosis, while inflammatory lesions were characterized by dermal inflammation and epidermal acanthosis, spongiosis, and parakeratosis. In conclusion, loss of epidermal Pparg alters a substantial set of genes that are associated with cutaneous inflammation, keratinization, and sebaceous gland function. The data indicate that epidermal PPARγ plays an important role in homeostatic epidermal function, particularly epidermal differentiation, barrier function, sebaceous gland development and function, and inflammatory signaling.


Assuntos
Dermatite/genética , Epiderme/metabolismo , PPAR gama/fisiologia , Fenômenos Fisiológicos da Pele/genética , Animais , Células Cultivadas , Dermatite/metabolismo , Dermatite/patologia , Dermatite/fisiopatologia , Epiderme/fisiologia , Homeostase/genética , Inflamação/genética , Inflamação/metabolismo , Inflamação/patologia , Camundongos , Camundongos da Linhagem 129 , Camundongos Endogâmicos C57BL , Camundongos Knockout , Especificidade de Órgãos/genética , PPAR gama/genética , PPAR gama/metabolismo
3.
Int J Mol Sci ; 22(13)2021 Jun 23.
Artigo em Inglês | MEDLINE | ID: mdl-34201667

RESUMO

Human plasma-derived bilayered skin substitutes were successfully used by our group to produce human-based in vitro skin models for toxicity, cosmetic, and pharmaceutical testing. However, mechanical weakness, which causes the plasma-derived fibrin matrices to contract significantly, led us to attempt to improve their stability. In this work, we studied whether an increase in fibrin concentration from 1.2 to 2.4 mg/mL (which is the useful fibrinogen concentration range that can be obtained from plasma) improves the matrix and, hence, the performance of the in vitro skin cultures. The results show that this increase in fibrin concentration indeed affected the mechanical properties by doubling the elastic moduli and the maximum load. A structural analysis indicated a decreased porosity for the 2.4 mg/mL hydrogels, which can help explain this mechanical behavior. The contraction was clearly reduced for the 2.4 mg/mL matrices, which also allowed for the growth and proliferation of primary fibroblasts and keratinocytes, although at a somewhat reduced rate compared to the 1.2 mg/mL gels. Finally, both concentrations of fibrin gave rise to organotypic skin cultures with a fully differentiated epidermis, although their lifespans were longer (25-35%) in cultures with more concentrated matrices, which improves their usefulness. These systems will allow the generation of much better in vitro skin models for the testing of drugs, cosmetics and chemicals, or even to "personalized" skin for the diagnosis or determination of the most effective treatment possible.


Assuntos
Diferenciação Celular , Derme/citologia , Epiderme/fisiologia , Fibrina/metabolismo , Hidrogéis/metabolismo , Queratinócitos/citologia , Tecidos Suporte/química , Proliferação de Células , Células Cultivadas , Derme/metabolismo , Fibroblastos/citologia , Fibroblastos/metabolismo , Humanos , Hidrogéis/química , Queratinócitos/metabolismo , Pele/citologia , Pele/metabolismo , Engenharia Tecidual
4.
Genes (Basel) ; 12(5)2021 05 18.
Artigo em Inglês | MEDLINE | ID: mdl-34069986

RESUMO

The transition of amniotes to a fully terrestrial lifestyle involved the adaptation of major molecular innovations to the epidermis, often in the form of epidermal appendages such as hair, scales and feathers. Feathers are diverse epidermal structures of birds, and their evolution has played a key role in the expansion of avian species to a wide range of lifestyles and habitats. As with other epidermal appendages, feather development is a complex process which involves many different genetic and protein elements. In mammals, many of the genetic elements involved in epidermal development are located at a specific genetic locus known as the epidermal differentiation complex (EDC). Studies have identified a homologous EDC locus in birds, which contains several genes expressed throughout epidermal and feather development. A family of avian EDC genes rich in aromatic amino acids that also contain MTF amino acid motifs (EDAAs/EDMTFs), that includes the previously reported histidine-rich or fast-protein (HRP/fp), an important marker in feather development, has expanded significantly in birds. Here, we characterize the EDAA gene family in birds and investigate the evolutionary history and possible functions of EDAA genes using phylogenetic and sequence analyses. We provide evidence that the EDAA gene family originated in an early archosaur ancestor, and has since expanded in birds, crocodiles and turtles, respectively. Furthermore, this study shows that the respective amino acid compositions of avian EDAAs are characteristic of structural functions associated with EDC genes and feather development. Finally, these results support the hypothesis that the genes of the EDC have evolved through tandem duplication and diversification, which has contributed to the evolution of the intricate avian epidermis and epidermal appendages.


Assuntos
Aves/genética , Aves/fisiologia , Epiderme/fisiologia , Família Multigênica/genética , Motivos de Aminoácidos/genética , Aminoácidos/genética , Animais , Biomarcadores/metabolismo , Evolução Molecular , Plumas/fisiologia , Mamíferos/genética , Mamíferos/fisiologia , Proteínas/genética , Sequências de Repetição em Tandem/genética
5.
Nat Commun ; 12(1): 2595, 2021 05 10.
Artigo em Inglês | MEDLINE | ID: mdl-33972536

RESUMO

Tissue regeneration is a process that recapitulates and restores organ structure and function. Although previous studies have demonstrated wound-induced hair neogenesis (WIHN) in laboratory mice (Mus), the regeneration is limited to the center of the wound unlike those observed in African spiny (Acomys) mice. Tissue mechanics have been implicated as an integral part of tissue morphogenesis. Here, we use the WIHN model to investigate the mechanical and molecular responses of laboratory and African spiny mice, and report these models demonstrate opposing trends in spatiotemporal morphogenetic field formation with association to wound stiffness landscapes. Transcriptome analysis and K14-Cre-Twist1 transgenic mice show the Twist1 pathway acts as a mediator for both epidermal-dermal interactions and a competence factor for periodic patterning, differing from those used in development. We propose a Turing model based on tissue stiffness that supports a two-scale tissue mechanics process: (1) establishing a morphogenetic field within the wound bed (mm scale) and (2) symmetry breaking of the epidermis and forming periodically arranged hair primordia within the morphogenetic field (µm scale). Thus, we delineate distinct chemo-mechanical events in building a Turing morphogenesis-competent field during WIHN of laboratory and African spiny mice and identify its evo-devo advantages with perspectives for regenerative medicine.


Assuntos
Epiderme/anatomia & histologia , Epiderme/metabolismo , Folículo Piloso/metabolismo , Morfogênese/fisiologia , Regeneração/fisiologia , Proteína 1 Relacionada a Twist/metabolismo , Cicatrização/fisiologia , Animais , Epiderme/fisiologia , Perfilação da Expressão Gênica , Folículo Piloso/anatomia & histologia , Folículo Piloso/fisiologia , Imuno-Histoquímica , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Análise em Microsséries , Microscopia de Força Atômica , Modelos Psicológicos , Morfogênese/genética , Murinae , RNA-Seq , Regeneração/genética , Medicina Regenerativa , Transdução de Sinais/genética , Transdução de Sinais/fisiologia , Análise Espaço-Temporal , Proteína 1 Relacionada a Twist/genética , Cicatrização/genética
6.
Int J Mol Sci ; 22(6)2021 Mar 16.
Artigo em Inglês | MEDLINE | ID: mdl-33809733

RESUMO

The skin epidermis is the outermost epithelial tissue that protects the body from the external environment [...].


Assuntos
Epiderme/fisiologia , Dermatite Atópica/patologia , Homeostase , Humanos , MicroRNAs/genética , MicroRNAs/metabolismo , Modelos Biológicos , Transdução de Sinais
7.
Int J Biol Macromol ; 182: 413-424, 2021 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-33798572

RESUMO

Most of the spray products in the market for wound healing applications are loaded with antibiotics that exert their antibacterial effect within the inflammatory stage of wound healing without demonstrating any effect in the subsequent proliferation stage. This study introduces a new aerosolized nanopowder (ANP) formula that not only exhibits antibacterial effect but also antioxidant and enhanced cell proliferation effects. Within the introduced ANP formula, Avicenna marina (Am) extract and neomycin (NM) antibiotic have been loaded within silk-fibroin nanoparticles (FB NPs). The Am has been extracted via different solvent systems, and investigated for its antioxidant and antibacterial activity as well as its ability to enhance cell proliferation. The physicochemical properties, size, zeta-potential and morphology of the prepared Am/FB NPs, NM/FB NPs and ANP formula were investigated. Besides, the ANP formula exhibited good antibacterial activities against Staphylococcus aureus, Methicillin resistant S. aureus, Pseudomonas aeruginosa and Resistant P. aeruginosa. Scratch wound healing assay on human fibroblast monolayers demonstrated 100% wound closure after 24 h upon using the ANP formula as compared to 70% wound closure for positive control (NM). The wound healing ability of the ANP formula has been further confirmed by histopathological evaluation of the wound site and depicted a marked increase in fibroblast proliferation and reduction of inflammatory cells after 15 days with a complete wound closure as compared to controls. The obtained results prove the beneficial effects of the Am extract on wound healing and introduce the developed multitask nanopowder formula as a potential wound healing spray.


Assuntos
Aerossóis/química , Fibroínas/química , Nanopartículas/química , Cicatrização , Animais , Antibacterianos/administração & dosagem , Antibacterianos/farmacologia , Bactérias/efeitos dos fármacos , Linhagem Celular , Proliferação de Células , Liberação Controlada de Fármacos , Epiderme/efeitos dos fármacos , Epiderme/fisiologia , Fibroblastos/efeitos dos fármacos , Humanos , Neomicina/administração & dosagem , Neomicina/farmacologia , Ratos
8.
J Drugs Dermatol ; 20(4): s10-s16, 2021 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-33852255

RESUMO

The skin barrier is a multifaceted microenvironment, comprised not only of structural and molecular components that maintain its integrity, but also a lipid matrix comprising an equimolar ratio of cholesterol, free fatty acids, and ceramides. Lipid abnormalities induced by environmental or pathological stimuli are often associated with impaired skin barrier function and integrity. Incorporation of skin lipids in skincare formulations to help fortify barrier function has become widespread. While there are resources available to study the barrier, a comprehensive evaluation of skin models, from in situ to in vivo, that focus on alterations of the lipid content, seems to be lacking. This article reviews current methods to evaluate the skin lipid barrier and touches upon the significance of using such models within the cosmetic field to study formulations that incorporate barrier lipids. J Drugs Dermatol. 20(4 Suppl):s10-16. doi:10.36849/JDD.S589B.


Assuntos
Cosméticos/administração & dosagem , Emolientes/administração & dosagem , Epiderme/efeitos dos fármacos , Higiene da Pele/métodos , Animais , Técnicas de Cultura de Células , Linhagem Celular , Ceramidas/administração & dosagem , Ceramidas/metabolismo , Colesterol/administração & dosagem , Colesterol/metabolismo , Cosméticos/química , Modelos Animais de Doenças , Emolientes/química , Epiderme/fisiologia , Epiderme/efeitos da radiação , Ácidos Graxos não Esterificados/administração & dosagem , Ácidos Graxos não Esterificados/metabolismo , Humanos , Técnicas de Cultura de Tecidos , Raios Ultravioleta/efeitos adversos , Perda Insensível de Água/efeitos dos fármacos
9.
Elife ; 102021 03 29.
Artigo em Inglês | MEDLINE | ID: mdl-33779546

RESUMO

While the mechanisms by which chemical signals control cell fate have been well studied, the impact of mechanical inputs on cell fate decisions is not well understood. Here, using the well-defined system of keratinocyte differentiation in the skin, we examine whether and how direct force transmission to the nucleus regulates epidermal cell fate. Using a molecular biosensor, we find that tension on the nucleus through linker of nucleoskeleton and cytoskeleton (LINC) complexes requires integrin engagement in undifferentiated epidermal stem cells and is released during differentiation concomitant with decreased tension on A-type lamins. LINC complex ablation in mice reveals that LINC complexes are required to repress epidermal differentiation in vivo and in vitro and influence accessibility of epidermal differentiation genes, suggesting that force transduction from engaged integrins to the nucleus plays a role in maintaining keratinocyte progenitors. This work reveals a direct mechanotransduction pathway capable of relaying adhesion-specific signals to regulate cell fate.


Assuntos
Epiderme/fisiologia , Mecanotransdução Celular/fisiologia , Lâmina Nuclear/fisiologia , Plaquinas/genética , Animais , Diferenciação Celular , Feminino , Integrinas/metabolismo , Lamina Tipo A/metabolismo , Camundongos , Plaquinas/metabolismo
10.
Int J Mol Sci ; 22(5)2021 Feb 28.
Artigo em Inglês | MEDLINE | ID: mdl-33670966

RESUMO

3-hydroxytyrosol (HT) is the main phenolic compound found in olive oil with known antioxidant, anti-inflammatory, and antimicrobial properties in several dermatological conditions, both when taken in the form of olive oil or pure in cosmeceutical formulations. To date, its direct effect on the wound healing process and the molecular mechanisms involved have not yet been elucidated. Thus, in the present study, we aimed to explore its effects in vitro in epidermal keratinocyte cultures focusing on the molecular mechanism implied. HT was able to induce keratinocyte proliferation in the low micromolar range, increasing the expression of cyclin dependent kinases fundamental for cell cycle progression such as CDK2 and CDK6. Furthermore, it increased cell migration through the activation of tissue remodeling factors such as matrix metalloproteinase-9 (MMP-9) protein. Then, we evaluated whether HT also showed antioxidant activity at this concentration range, protecting from H2O2-induced cytotoxicity. The HT prevented the activation of ATM serine/threonine kinase (ATM), Checkpoint kinase 1 (Chk1), Checkpoint kinase 2 (Chk2), and p53, reducing the number of apoptotic cells. Our study highlighted novel pharmacological properties of HT, providing the first evidence of its capability to induce keratinocyte migration and proliferation required for healing processes and re-epithelialization.


Assuntos
Movimento Celular , Proliferação de Células , Epiderme/fisiologia , Sequestradores de Radicais Livres/farmacologia , Radicais Livres/efeitos adversos , Queratinócitos/fisiologia , Álcool Feniletílico/análogos & derivados , Antioxidantes/farmacologia , Células Cultivadas , Epiderme/efeitos dos fármacos , Humanos , Peróxido de Hidrogênio/efeitos adversos , Queratinócitos/citologia , Queratinócitos/efeitos dos fármacos , Oxidantes/efeitos adversos , Álcool Feniletílico/farmacologia , Transdução de Sinais , Cicatrização
11.
Nat Commun ; 12(1): 784, 2021 02 04.
Artigo em Inglês | MEDLINE | ID: mdl-33542242

RESUMO

In adult tissue, stem and progenitor cells must tightly regulate the balance between proliferation and differentiation to sustain homeostasis. How this exquisite balance is achieved is an area of active investigation. Here, we show that epidermal genes, including ~30% of induced differentiation genes already contain stalled Pol II at the promoters in epidermal stem and progenitor cells which is then released into productive transcription elongation upon differentiation. Central to this process are SPT6 and PAF1 which are necessary for the elongation of these differentiation genes. Upon SPT6 or PAF1 depletion there is a loss of human skin differentiation and stratification. Unexpectedly, loss of SPT6 also causes the spontaneous transdifferentiation of epidermal cells into an intestinal-like phenotype due to the stalled transcription of the master regulator of epidermal fate P63. Our findings suggest that control of transcription elongation through SPT6 plays a prominent role in adult somatic tissue differentiation and the inhibition of alternative cell fate choices.


Assuntos
Diferenciação Celular/genética , Epiderme/fisiologia , Elongação da Transcrição Genética , Fatores de Transcrição/metabolismo , Células-Tronco Adultas/fisiologia , Transdiferenciação Celular/genética , Células Cultivadas , Sequenciamento de Cromatina por Imunoprecipitação , Técnicas de Silenciamento de Genes , Humanos , Recém-Nascido , Queratinócitos , Masculino , Cultura Primária de Células , Regiões Promotoras Genéticas , RNA Polimerase II/metabolismo , RNA Interferente Pequeno/metabolismo , RNA-Seq , Técnicas de Cultura de Tecidos , Fatores de Transcrição/genética , Proteínas Supressoras de Tumor/metabolismo
12.
Biomed Eng Online ; 20(1): 22, 2021 Feb 17.
Artigo em Inglês | MEDLINE | ID: mdl-33596908

RESUMO

BACKGROUND: The detection and dissection of epidermal subgroups could lead to an improved understanding of skin homeostasis and wound healing. Flow cytometric analysis provides an effective method to detect the surface markers of epidermal cells while producing high-dimensional data files. METHODS: A 9-color flow cytometric panel was optimized to reveal the heterogeneous subgroups in the epidermis of human skin. The subsets of epidermal cells were characterized using automated methods based on dimensional reduction approaches (viSNE) and clustering with Spanning-tree Progression Analysis of Density-normalized Events (SPADE). RESULTS: The manual analysis revealed differences in epidermal distribution between body sites based on a series biaxial gating starting with the expression of CD49f and CD29. The computational analysis divided the whole epidermal cell population into 25 clusters according to the surface marker phenotype with SPADE. This automatic analysis delineated the differences between body sites. The consistency of the results was confirmed with PhenoGraph. CONCLUSION: A multicolor flow cytometry panel with a streamlined computational analysis pipeline is a feasible approach to delineate the heterogeneity of the epidermis in human skin.


Assuntos
Epiderme/fisiologia , Citometria de Fluxo/métodos , Pele/citologia , Algoritmos , Análise por Conglomerados , Cor , Simulação por Computador , Humanos , Aprendizado de Máquina , Reconhecimento Automatizado de Padrão , Fenótipo , Software
13.
Sci Rep ; 11(1): 1688, 2021 01 18.
Artigo em Inglês | MEDLINE | ID: mdl-33462350

RESUMO

Impaired wound healing is an immense medical challenge, and while autologous skin grafting remains the "gold-standard" therapeutic option for repairing wounds that cannot be closed by primary or secondary intention, it is limited by substantial donor site morbidity. We previously developed the alternative approach of harvesting full-thickness skin tissue in the form of "micro skin tissue columns" (MSTCs), without causing scarring or any other long-term morbidity. In this study we investigated how MSTC treatment affects the different cellular processes involved in wound healing. We found that MSTC-derived cells were able to remodel and repopulate the wound volume, and positively impact multiple aspects of the wound healing process, including accelerating re-epithelialization by providing multiple cell sources throughout the wound area, increasing collagen deposition, enhancing dermal remodeling, and attenuating the inflammatory response. These effects combined to enhance both epidermal and dermal wound healing. This MSTC treatment approach was designed for practical clinical use, could convey many benefits of autologous skin grafting, and avoids the major drawback of donor site morbidity.


Assuntos
Epiderme/fisiologia , Transplante de Pele/métodos , Pele/citologia , Cicatrização/fisiologia , Animais , Feminino , Reepitelização , Suínos , Transplante Autólogo
14.
ACS Appl Mater Interfaces ; 13(4): 5660-5667, 2021 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-33467850

RESUMO

Conformal integration of an epidermal device with the skin, as well as sweat and air permeability, are crucial to reduce stress on biological tissues. Nanofiber-based porous mesh structures (breathable devices) are commonly utilized to prevent skin problems. Noble metals are normally deposited on nanomesh substrates to form breathable electrodes. However, these are expensive and require high-vacuum processes involving time-consuming multistep procedures. Organic materials are suitable alternatives that can be simply processed in solution. We report a simple, cost-effective, mechanically biocompatible, and breathable organic epidermal electrode for biometric devices. Poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate) (PEDOT:PSS) is sprayed on a nanofiber-mesh structure, treated using only heat and water to enhance its biocompatibility and conductivity, and used as the electrode. The treatment is accomplished using an autoclave, simultaneously reducing the electrical resistance and sterilizing the electrode for practical use. This research can lead to affordable and biocompatible epidermal electrodes with improved suitability for various biomedical applications.


Assuntos
Materiais Biocompatíveis/química , Epiderme/fisiologia , Nanofibras/química , Poliestirenos/química , Tiofenos/química , Dispositivos Eletrônicos Vestíveis , Biometria/instrumentação , Elasticidade , Condutividade Elétrica , Eletrodos , Humanos , Teste de Materiais
15.
Gene ; 775: 145440, 2021 Apr 05.
Artigo em Inglês | MEDLINE | ID: mdl-33482282

RESUMO

Tubgcp3/GCP3 (The centrosomal protein γ-tubulin complex protein 3) is a component of the γ-tubulin small complexes (γ-TuSCs) and γ-tubulin ring complexes (γ-TuRCs), which play critical roles in mitotic spindle formation during mitosis. However, its function in stem cell development has not been thoroughly elucidated. The planarian flatworm, which contains a large number of adult somatic stem cells (neoblasts), is a unique model to study stem cell lineage development in vivo. Here, we identified a homolog of Tubgcp3 in planarian Dugesia japonica, and found that Tubgcp3 is required for the maintenance of epidermal lineage. RNAi targeting Tubgcp3 resulted in tissue homeostasis and regeneration defect. Knockdown of Tubgcp3 reduced cell divisions and led to a loss of the mature epidermal cells. Our findings indicate that Tubgcp3 is a mitotic regulator and plays a crucial role in planarian epidermal differentiation.


Assuntos
Epiderme/fisiologia , Proteínas Associadas aos Microtúbulos/genética , Animais , Diferenciação Celular , Proliferação de Células , Clonagem Molecular , Proteínas de Helminto/genética , Proteínas de Helminto/metabolismo , Proteínas Associadas aos Microtúbulos/metabolismo , Planárias , Regeneração
16.
J Wound Care ; 30(1): 41-53, 2021 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-33439080

RESUMO

BACKGROUND: Sub-epidermal moisture (SEM) is a measurable biomarker detecting early pressure damage in order to objectively support current 'gold standard' skin tissue assessments (STA) for the detection of deep and early-stage pressure-induced injuries or ulcers (PI/PUs). OBJECTIVE: A multi-site, dual arm, cross sectional, retrospective study was conducted to evaluate the sensitivity, specificity and clinical utility of spatial variation in SEM readings between healthy and damaged skin tissue. METHOD: The study enrolled 175 subjects: 125 with confirmed PI/PUs or suspected deep tissue injury (sDTI), and 50 confirmed healthy subjects. Expert principal investigators and PI/PU healthcare practitioners (HCPs) evaluating all subjects were trained in SEM measurements but blinded to clinical interpretation of SEM readings. Sequential and spatial SEM readings of the sacrum and heels, subjects' demographic data, STAs, risk assessment tool scores (RATS), pain assessment and potential confounders were recorded. Independent statistical analyses were performed. RESULTS: Mean spatial SEM measures within subjects with healthy tissue and within subjects with damaged tissue were statistically similar. Mean spatial SEM measures within anatomies of subjects with damaged tissue were significantly different (p<0.05). There was no significant difference between spatial readings in healthy subjects. Algorithms computing a range of SEM delta thresholds indicated a sensitivity of 82-87% and a specificity of 51-88% at an SEM delta ≥0.6. Receiver operating characteristic (ROC) curves computed areas under the curve (AUC) of 0.7809-0.9181 (95% CI: 0.7221-0.8817, 0.8397-0.9545, p<0.0001) exceeding clinical judgement. CONCLUSION: These SEM data augment clinical decision-making for developing intact skin PI/PUs including sDTIs and Stage I PI/PUs. Informing HCPs of this subclinical, non-visible skin and tissue damage and providing opportunities for alternative PI/PU care pathways is an exciting prospect.


Assuntos
Algoritmos , Epiderme/fisiologia , Lesão por Pressão/diagnóstico , Adulto , Estudos Transversais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Valor Preditivo dos Testes , Estudos Retrospectivos , Medição de Risco , Índice de Gravidade de Doença
17.
Commun Biol ; 4(1): 114, 2021 01 25.
Artigo em Inglês | MEDLINE | ID: mdl-33495490

RESUMO

Chondroitin sulfates are implicated in epidermal biology, but functional significance of chondroitin sulfates remains unclear. Here, we report that chondroitin 6-sulfate is important for the maintenance of epidermal homeostasis. Mice deficient in chondroitin 6-O-sulfotransferase-1 (C6st-1), which is involved in biosynthesis of chondroitin 6-sulfate, exhibited keratinocyte hyperproliferation and impaired skin permeability barrier function. Chondroitin 6-sulfate directly interacted with the EGF receptor and negatively controlled ligand-induced EGF receptor signaling. Normal function of hyperproliferative C6st-1-knockout mouse-derived keratinocytes was rescued by treatment with exogenous chondroitin 6-sulfate. Epidermal hyperplasia, induced using imiquimod, was more severe in C6st-1-knockout mice than in C6st-1 wild-type mice. Taken together, these findings indicate that chondroitin 6-sulfate represses keratinocyte proliferation in normal skin, and that the expression level of C6st-1 may be associated with susceptibility to psoriasis.


Assuntos
Proliferação de Células/efeitos dos fármacos , Sulfatos de Condroitina/farmacologia , Queratinócitos/efeitos dos fármacos , Psoríase , Animais , Células Cultivadas , Sulfatos de Condroitina/metabolismo , Epiderme/efeitos dos fármacos , Epiderme/fisiologia , Predisposição Genética para Doença , Células HaCaT , Humanos , Queratinócitos/fisiologia , Camundongos , Camundongos Knockout , Psoríase/genética , Psoríase/metabolismo , Psoríase/patologia , Pele/citologia , Pele/efeitos dos fármacos , Sulfotransferases/genética , Sulfotransferases/metabolismo
18.
Biol Res Nurs ; 23(1): 75-81, 2021 01.
Artigo em Inglês | MEDLINE | ID: mdl-32648469

RESUMO

BACKGROUND: Preventing recurrent pressure injuries (RPIs) is one of the important challenges faced in healthcare, but the risk factors of RPIs have not been fully revealed. This study aims to explore factors associated with RPIs, by focusing on skin physiology and its microbiome as local factors crucial for the health of healed tissue after pressure injury healing. METHODS: This prospective observational study was conducted in a long-term care facility in Japan with patients whose PIs had healed within 1 month. Skin physiology was evaluated by stratum corneum (SC) hydration, pH, and transepidermal water loss. Skin bacteria was collected by tape stripping, followed by 16S ribosomal RNA-based metagenomics analysis. These parameters were evaluated every two weeks over a period of six weeks. RESULTS: A total of 30 patients were included in this study, and 8 patients (26.7%) had an RPI within 6 weeks. In this study, significantly lower SC hydration and a higher rate of Staphylococcus species on the healed site were found in the RPI group. DISCUSSION: A high rate of RPIs (about one in four) points out the necessity of a further care strategy on the healed PIs. Lower skin hydration and/or the increase in Staphylococcus bacteria may have a potential to be used as a biomarker for the prediction of RPIs, or may be an intervention point for the prevention of RPIs by, for example, skin cleansing with moisturizing care.


Assuntos
Microbiota , Lesão por Pressão/microbiologia , Lesão por Pressão/patologia , Fenômenos Fisiológicos da Pele , Pele/microbiologia , Idoso , Idoso de 80 Anos ou mais , Epiderme/fisiologia , Feminino , Humanos , Japão/epidemiologia , Assistência de Longa Duração/estatística & dados numéricos , Masculino , Lesão por Pressão/enfermagem , Estudos Prospectivos , Recidiva , Pele/patologia
19.
ACS Appl Mater Interfaces ; 12(50): 56361-56371, 2020 Dec 16.
Artigo em Inglês | MEDLINE | ID: mdl-33270412

RESUMO

Epidermal electronics is regarded as the next-generation technology, and graphene is a promising electrode, which is a key building block of such devices. However, graphene has a tendency to crack at small strains with a rapidly increased resistance upon stretching. Here, to enable graphene applicable in epidermal electronics, we designed a novel graphene structure that is molybdenum chloride (MoCl5)-intercalated few-layer graphene (Mo-FLG) fabricated in a confined environment. In the case of bilayer graphene (BLG), MoCl5-intercalated bilayer graphene (Mo-BLG) exhibited a low sheet resistance of 40 Ω/square (sq) at a transmittance of 80%. Due to the self-barrier doping effect, the sheet resistance increased to only 60 Ω/sq after exposing to the atmosphere over 1 month. Transferred onto elastomer substrates, Mo-BLG can work as an electrode up to 80% strain and maintain a high conductivity that is durable over 2000 cycles at 30% strain. This mechano-electrostability is attributed to the special intercalated structure where the intercalated dopants act as lubricants to weaken the layer-layer interaction and allow a certain degree of sliding, as well as electrical crack-connectors to bridge the cracked domains at a high strain. Mo-BLG can be applied as epidermal electrodes to monitor electrophysiological signals such as electrocardiogram (ECG), electrooculogram (EOG), electroencephalography (EEG), and surface electromyogram (sEMG) with high signal-to-noise ratios (SNRs) comparable to commercial Ag/AgCl electrode. This is the first demonstration of epidermal electrodes based on intercalation-doped graphene applied in health monitoring, shedding light on the future development of graphene-based epidermal electronics.


Assuntos
Eletrocardiografia/instrumentação , Eletroencefalografia/instrumentação , Eletromiografia/instrumentação , Grafite/química , Cloretos/química , Elastômeros/química , Condutividade Elétrica , Eletrodos , Epiderme/fisiologia , Humanos , Molibdênio/química , Razão Sinal-Ruído
20.
Med Sci (Paris) ; 36(12): 1155-1162, 2020 Dec.
Artigo em Francês | MEDLINE | ID: mdl-33296632

RESUMO

The skin is a sentinel organ making easily visible the passing of time. Chronological and environmental aging weakens skin structure and functions. The skin barrier, the elastic and mechanical properties of the cutaneous tissue as well as its vascular reactivity are impacted by aging. The barrier dysfunction in aged skin is caused by defects in epidermal keratinocytes renewal and differentiation notably linked to abnormal expression of microRNAs regulating cell death and autophagy. An abnormal balance between synthesis and degradation of matrix proteins modifies the mechanical properties of the dermis in aged skin. Finally, a reduction of the vascular reactivity linked to endothelial dysfunctions is observed in elderly people. These biological processes can be targeted by therapeutic approaches either topical or systemic, especially using anti-oxydants or senolytics. These anti-aging strategies might contribute to restore, at least in part, the functional integrity of aged skin.


Assuntos
Envelhecimento/fisiologia , Envelhecimento da Pele/fisiologia , Fenômenos Fisiológicos da Pele , Terapias em Estudo/tendências , Idoso , Idoso de 80 Anos ou mais , Envelhecimento/efeitos dos fármacos , Cosmecêuticos/uso terapêutico , Fármacos Dermatológicos/uso terapêutico , Epiderme/efeitos dos fármacos , Epiderme/fisiologia , Humanos , Microvasos/efeitos dos fármacos , Microvasos/fisiologia , Microvasos/fisiopatologia , Pele/irrigação sanguínea , Pele/efeitos dos fármacos , Pele/patologia , Envelhecimento da Pele/efeitos dos fármacos , Envelhecimento da Pele/patologia , Fenômenos Fisiológicos da Pele/efeitos dos fármacos , Terapias em Estudo/métodos
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...