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1.
Nat Commun ; 11(1): 665, 2020 01 31.
Artigo em Inglês | MEDLINE | ID: mdl-32005801

RESUMO

Injury, surgery, and disease often disrupt tissues and it is the process of regeneration that aids the restoration of architecture and function. Regeneration can occur through multiple strategies including stem cell expansion, transdifferentiation, or proliferation of differentiated cells. We have identified a case of regeneration in Xenopus embryonic aggregates that restores a mucociliated epithelium from mesenchymal cells. Following disruption of embryonic tissue architecture and assembly of a compact mesenchymal aggregate, regeneration first restores an epithelium, transitioning from mesenchymal cells at the surface of the aggregate. Cells establish apico-basal polarity within 5 hours and a mucociliated epithelium within 24 hours. Regeneration coincides with nuclear translocation of the putative mechanotransducer YAP1 and a sharp increase in aggregate stiffness, and regeneration can be controlled by altering stiffness. We propose that regeneration of a mucociliated epithelium occurs in response to biophysical cues sensed by newly exposed cells on the surface of a disrupted mesenchymal tissue.


Assuntos
Epiderme/química , Epiderme/fisiologia , Xenopus laevis/embriologia , Animais , Fenômenos Biomecânicos , Epiderme/embriologia , Epitélio/química , Epitélio/embriologia , Epitélio/fisiologia , Mesoderma/química , Mesoderma/embriologia , Mesoderma/fisiologia , Regeneração , Xenopus laevis/fisiologia
2.
J Acupunct Meridian Stud ; 13(1): 33-38, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31743773

RESUMO

The primo vascular system (PVS) is reported to have a periductium composed of cells with spherical or spindle-shaped nuclei and abundant cytoplasm. However, little is known about these periductium cells. In this study, we examined the morphological features of cells covering the PVS tissue isolated from the surface of abdominal organs of rats. By hematoxylin and eosin (H&E) staining, we observed a layer of dark nuclei on the basement membrane at the borders of the sections of primo node (PN), primo vessel (PV), and their subunits. The nuclei appeared thin and linear (10-14 µm), elliptical (8-10 × 3-4 µm), and round (5-7 µm). The borders of the PVS tissue sections were immunostained with a selective antibody for mesothelial cells (MCs). Areas of immunoreactivity overlapped with the flattened cells are shown by hematoxylin and eosin staining. By scanning electron microscopy, we further identified elliptical (11 × 21 µm) and rectangular squamous MCs (length, 10 µm). There were numerous stomata (∼200 nm) and microparticles (20-200 nm) on the surface of the PVS MCs. In conclusion, this study presents the novel finding that the PVS periductium is composed of squamous MCs. These cells tightly line the luminal surface of the PVS tissue, including PNs, PVs, and small branches of the PVs in the abdominal cavity. These results will help us to understand the physiological roles such as hyaluronan secretion and the fine structure of PVS tissue.


Assuntos
Epitélio/anatomia & histologia , Epitélio/fisiologia , Meridianos , Animais , Epitélio/química , Masculino , Ratos , Ratos Sprague-Dawley , Coloração e Rotulagem
3.
Dev Growth Differ ; 61(9): 447-456, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31713234

RESUMO

Wound epidermis (WE) and the apical epithelial cap (AEC) are believed to trigger regeneration of amputated appendages such as limb and tail in amphibians by producing certain secreted signaling molecules. To date, however, only limited information about the molecular signatures of these epidermal structures is available. Here we used a transgenic Xenopus laevis line harboring the enhanced green fluorescent protein (egfp) gene under control of an es1 gene regulatory sequence to isolate WE/AEC cells by performing fluorescence-activated cell sorting during the time course of tail regeneration (day 1, day 2, day 3 and day 4 after amputation). Time-course transcriptome analysis of these isolated WE/AEC cells revealed that more than 8,000 genes, including genes involved in signaling pathways such as those of reactive oxygen species, fibroblast growth factor (FGF), canonical and non-canonical Wnt, transforming growth factor ß (TGF ß) and Notch, displayed dynamic changes of their expression during tail regeneration. Notably, this approach enabled us to newly identify seven secreted signaling molecule genes (mdk, fstl, slit1, tgfß1, bmp7.1, angptl2 and egfl6) that are highly expressed in tail AEC cells. Among these genes, five (mdk, fstl, slit1, tgfß1 and bmp7.1) were also highly expressed in limb AEC cells but the other two (angptl2 and egfl6) are specifically expressed in tail AEC cells. Interestingly, there was no expression of fgf8 in tail WE/AEC cells, whose expression and pivotal role in limb AEC cells have been reported previously. Thus, we identified common and different properties between tail and limb AEC cells.


Assuntos
Proteínas de Fluorescência Verde/genética , Transdução de Sinais/genética , Proteínas de Xenopus/genética , Animais , Epitélio/química , Citometria de Fluxo , Perfilação da Expressão Gênica , Análise de Sequência de RNA , Xenopus laevis
4.
Soft Matter ; 15(44): 9133-9149, 2019 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-31674622

RESUMO

Recent work on particle-based models of tissues has suggested that any finite rate of cell division and cell death is sufficient to fluidize an epithelial tissue. At the same time, experimental evidence has indicated the existence of glassy dynamics in some epithelial layers despite continued cell cycling. To address this discrepancy, we quantify the role of cell birth and death on glassy states in confluent tissues using simulations of an active vertex model that includes cell motility, cell division, and cell death. Our simulation data is consistent with a simple ansatz in which the rate of cell-life cycling and the rate of relaxation of the tissue in the absence of cell cycling contribute independently and additively to the overall rate of cell motion. Specifically, we find that a glass-like regime with caging behavior indicated by subdiffusive cell displacements can be achieved in systems with sufficiently low rates of cell cycling.


Assuntos
Simulação por Computador , Células Epiteliais/citologia , Epitélio/química , Modelos Biológicos , Apoptose , Fenômenos Biomecânicos , Movimento Celular , Células Epiteliais/química , Células Epiteliais/fisiologia , Cinética , Mitose , Transição de Fase
5.
Gene ; 695: 101-112, 2019 May 05.
Artigo em Inglês | MEDLINE | ID: mdl-30763666

RESUMO

The fluted giant clam, Tridacna squamosa, lives in symbiosis with photosynthetic zooxanthellae, and can engage in light-enhanced growth and shell formation. Light-enhanced shell formation necessitates the elimination of excess H+ from the extrapallial fluid adjacent to the shell. This study aimed to clone Na+/H+Exchanger (NHE) from the whitish inner mantle adjacent to the extrapallial fluid of T. squamosa, to determine its cellular and subcellular localization, and to evaluate the effect of light exposure on its mRNA expression level and protein abundance therein. The complete coding cDNA sequence of NHE obtained was identified as a homolog of beta NHE (ßNHE-like). It consisted of 2925 bp, encoding for a polypeptide of 974 amino acids and 107.1 kDa, and was expressed predominantly in the inner mantle. There, ßNHE-like was localized in the apical membrane of the seawater-facing epithelium by immunofluorescence microscopy. After exposure to light for 12 h, the seawater-facing epithelium of the inner mantle displayed consistently stronger immunostaining than that of the control exposed to 12 h of darkness. Western blotting confirmed that light exposure significantly enhanced the protein abundance of ßNHE-like in the inner mantle. These results denote that some of the excess H+ generated during light-enhanced shell formation can be excreted through the light-dependent ßNHE-like of the seawater-facing epithelium to minimize the impact on the whole-body pH. Importantly, the excreted H+ could dehydrate exogenous HCO3-, and facilitate the absorption of inorganic carbon through the seawater-facing epithelium dedicated for light-enhanced shell formation due to its close proximity with the shell-facing epithelium. NUCLEOTIDE SYMBOL COMBINATIONS: Pairs: R = A/G; W = A/T; Y = C/T. Triples: D = A/G/T.


Assuntos
Bivalves/genética , Trocadores de Sódio-Hidrogênio/genética , Simbiose/genética , Sequência de Aminoácidos/genética , Animais , Bivalves/fisiologia , Clonagem Molecular , Epitélio/química , Epitélio/metabolismo , Luz , Fases de Leitura Aberta/genética , Fotossíntese/genética , RNA Mensageiro/genética , Água do Mar/microbiologia , Trocadores de Sódio-Hidrogênio/química
6.
Sci Rep ; 9(1): 90, 2019 01 14.
Artigo em Inglês | MEDLINE | ID: mdl-30643202

RESUMO

P120 catenin (p120) is a non-redundant master regulatory protein of cadherin-based cell-cell junctions, intracellular signaling, and tissue homeostasis and repair. Alternative splicing can generate p120 isoforms 1 and 3 (p120-1 and p120-3), which are implicated in non-overlapping functions by differential expression regulation and unique interactions in different cell types, with often predominant expression of p120-1 in mesenchymal cells, and p120-3 generally prevalent in epithelial cells. However, the lack of specific p120-3 protein detection has precluded analysis of their relative abundance in tissues. Here, we have developed a p120-3 isoform-specific antibody and analyzed the p120-3 localization relative to p120-1 in human tissues. p120-3 but not p120-1 is highly expressed in cell-cell junctions of simple gastrointestinal epithelia such as colon and stomach, and the acini of salivary glands and the pancreas. Conversely, the basal layer of the epidermis and hair follicles expressed p120-1 with reduced p120-3, whereas most other epithelia co-expressed p120-3 and p120-1, including bronchial epithelia and mammary luminal epithelial cells. These data provide an inventory of tissue-specific p120 isoform expression and suggest a link between p120 isoform expression and epithelial differentiation.


Assuntos
Cateninas/análise , Epitélio/química , Isoformas de Proteínas/análise , Transcriptoma , Humanos , Imunoensaio , Junções Intercelulares/química
7.
Kidney Blood Press Res ; 43(6): 1822-1831, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30537749

RESUMO

BACKGROUND/AIMS: Hyperglycemia and hyperuricemia are two major disorders of Metabolic syndrome. Kidney plays a crucial role in maintaining the homeostasis of uric acid and glucose. The aim of the study was to examine the changes of renal glucose and uric acid transporters in animals with metabolic syndrome. METHODS: Sprague-Dawley rats were fed with high fructose diet (60%) for 3 months (FR-3) and 5 months (FR-5). At the end study, serum and urine biochemical data were compared. Gene expression and protein abundance of renal GLUT1, GLUT2, GLUT9, SGLT1, SGLT2, UAT and URAT1 was investigated by using RT-PCR and immunohistochemical staining. RESULTS: Metabolic syndrome was induced by high-fructose diet. Systolic blood pressure and proteinuria was significantly increased in FR-5 animals. In kidney tissue, gene expression of GLUT2 and SGLT2 increased significantly in a time dependent manner. GLUT9, SGLT1 and UAT were also significantly upregulated in FR-5. Immunohistochemical study showed a significant increase of SGLT1 in both FR-3 (413.5 ± 88.3% of control, p< 0.001) and FR-5 (677.6 ± 26.5% of control, p< 0.001). Also, SGLT2 protein was increased in both FR-3 (643.1 ± 41.3% of control, p< 0.001) and FR-5 (563.3 ± 21.7% of control, p< 0.001). Fructose rich food also induced increase of UAT by nearly 5-fold in both FR-3 and FR-5 (both p< 0.05) and more than 3-fold of GLUT-9 in FR-3 and FR-5 (both p< 0.05). CONCLUSION: Long term high fructose diet induced metabolic syndrome with increased blood pressure and proteinuria in rats. Metabolic syndrome was associated with dual increase in renal glucose and uric acid transporters, including SGLT1, SGLT2, GLUT2, GLUT9 and UAT.


Assuntos
Frutose/efeitos adversos , Síndrome Metabólica/metabolismo , Transportadores de Ânions Orgânicos/metabolismo , Proteínas de Transporte de Sódio-Glucose/metabolismo , Animais , Epitélio/química , Rim/química , Rim/citologia , Síndrome Metabólica/induzido quimicamente , Ratos , Ratos Sprague-Dawley
8.
Curr Opin Genet Dev ; 51: 96-102, 2018 08.
Artigo em Inglês | MEDLINE | ID: mdl-30216753

RESUMO

Epithelium undergoes complex deformations during morphogenesis. Many of these deformations rely on the remodelling of apical cell junctions by actomyosin-based contractile force and this has been a major research interest for many years. Recent studies have shown that cells can use additional mechanisms that are not directly driven by actomyosin contractility to alter cell shape and movement, in three-dimensional (3D) space and time. In this review, we focus on a number of these mechanisms, including basolateral cellular protrusion, lateral shift of cell polarity, cytoplasmic flow, regulation of cell volume, and force transmission between cell-cell adhesion and cell-extracellular matrix adhesion, and describe how they underlie Drosophila epithelia deformations.


Assuntos
Actomiosina/química , Drosophila/genética , Epitélio/química , Morfogênese/genética , Actomiosina/genética , Animais , Polaridade Celular/genética , Drosophila/crescimento & desenvolvimento , Drosophila/metabolismo , Epitélio/metabolismo , Contração Muscular/genética , Contração Muscular/fisiologia
9.
Curr Opin Genet Dev ; 51: 78-87, 2018 08.
Artigo em Inglês | MEDLINE | ID: mdl-30077073

RESUMO

We review recent developments in the understanding of the biomechanics of apicomedial actomyosin and how its contractility can tense and deform tissue. Myosin pulses are driven by a biochemical oscillator but how they are modulated by the mechanical context remains unclear. On the other hand, the emergence of tissue behaviour is highly dependent on the material properties of actin, on how strongly components are connected and on the influence of neighbouring tissues. We further review the use of constitutive equations in exploring the mechanics of epithelial apices dominated by apicomedial Myosin contractility.


Assuntos
Actinas/química , Actomiosina/química , Epitélio/química , Miosinas/química , Actomiosina/metabolismo , Fenômenos Biomecânicos , Epitélio/metabolismo , Humanos
10.
Curr Opin Genet Dev ; 51: 88-95, 2018 08.
Artigo em Inglês | MEDLINE | ID: mdl-30103186

RESUMO

Epithelial cell rearrangements and cell shape changes are fundamental mechanisms by which cells build and shape elaborate and diverse tissue architectures from simple tissue sheets. These cell behaviors are regulated by a complex interplay between physical and biochemical mechanisms, many of which have been uncovered in recent studies in Drosophila. While the regulation of these cell behaviors is still under investigation, emerging technologies are being used to gain experimental control over these behaviors, opening new possibilities for designing and engineering tissue structures. Analysis of the biophysical mechanisms governing cell shape and movement will be crucial for understanding morphogenesis and for harnessing this knowledge to build tissues of precise shapes and structures for basic science and engineering applications.


Assuntos
Forma Celular/fisiologia , Células Epiteliais/química , Epitélio/química , Morfogênese/genética , Fenômenos Biofísicos , Forma Celular/genética , Células Epiteliais/metabolismo , Epitélio/metabolismo
11.
Endocrinology ; 159(9): 3331-3339, 2018 09 01.
Artigo em Inglês | MEDLINE | ID: mdl-30060183

RESUMO

We and others have reported that taste cells in taste buds express many peptides in common with cells in the gut and islets of Langerhans in the pancreas. Islets and taste bud cells express the hormones glucagon and ghrelin, the same ATP-sensitive potassium channel responsible for depolarizing the insulin-secreting ß cell during glucose-induced insulin secretion, as well as the propeptide-processing enzymes PC1/3 and PC2. Given the common expression of functionally specific proteins in taste buds and islets, it is surprising that no one has investigated whether insulin is synthesized in taste bud cells. Using immunofluorescence, we demonstrated the presence of insulin in mouse, rat, and human taste bud cells. By detecting the postprocessing insulin molecule C-peptide and green fluorescence protein (GFP) in taste cells of both insulin 1-GFP and insulin 2-GFP mice and the presence of the mouse insulin transcript by in situ hybridization, we further proved that insulin is synthesized in individual taste buds and not taken up from the parenchyma. In addition to our cytology data, we measured the level of insulin transcript by quantitative RT-PCR in the anterior and posterior lingual epithelia. These analyses showed that insulin is translated in the circumvallate and foliate papillae in the posterior, but only insulin transcript was detected in the anterior fungiform papillae of the rodent tongue. Thus, some taste cells are insulin-synthesizing cells generated from a continually replenished source of precursor cells in the adult mammalian lingual epithelium.


Assuntos
Expressão Gênica , Insulina/biossíntese , Insulina/genética , Papilas Gustativas/metabolismo , Animais , Diabetes Mellitus/metabolismo , Diabetes Mellitus Experimental/metabolismo , Ensaio de Imunoadsorção Enzimática , Epitélio/química , Epitélio/metabolismo , Feminino , Humanos , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos NOD , Biossíntese de Proteínas , RNA Mensageiro/análise , Ratos , Ratos Sprague-Dawley , Papilas Gustativas/química , Transcrição Genética
12.
Biol Lett ; 14(6)2018 06.
Artigo em Inglês | MEDLINE | ID: mdl-29899125

RESUMO

The giant clam Tridacna crocea, native to Indo-Pacific coral reefs, is noted for its unique ability to bore fully into coral rock and is a major agent of reef bioerosion. However, T. crocea's mechanism of boring has remained a mystery despite decades of research. By exploiting a new, two-dimensional pH-sensing technology and manipulating clams to press their presumptive boring tissue (the pedal mantle) against pH-sensing foils, we show that this tissue lowers the pH of surfaces it contacts by greater than or equal to 2 pH units below seawater pH day and night. Acid secretion is likely mediated by vacuolar-type H+-ATPase, which we demonstrate (by immunofluorescence) is abundant in the pedal mantle outer epithelium. Our discovery of acid secretion solves this decades-old mystery and reveals that, during bioerosion, T. crocea can liberate reef constituents directly to the soluble phase, rather than producing sediment alone as earlier assumed.


Assuntos
Bivalves/metabolismo , Epitélio/química , Ácidos/metabolismo , Animais , Bivalves/química , Recifes de Corais , Concentração de Íons de Hidrogênio , ATPases Translocadoras de Prótons/análise
13.
Int J Mol Sci ; 19(5)2018 May 10.
Artigo em Inglês | MEDLINE | ID: mdl-29748496

RESUMO

Calcium-activated chloride secretion in epithelial tissues has been described for many years. However, the molecular identity of the channel responsible for the Ca2+-activated Cl− secretion in epithelial tissues has remained a mystery. More recently, TMEM16A has been identified as a new putative Ca2+-activated Cl− channel (CaCC). The primary goal of this article will be to review the characterization of TMEM16A, as it relates to the physical structure of the channel, as well as important residues that confer voltage and Ca2+-sensitivity of the channel. This review will also discuss the role of TMEM16A in epithelial physiology and potential associated-pathophysiology. This will include discussion of developed knockout models that have provided much needed insight on the functional localization of TMEM16A in several epithelial tissues. Finally, this review will examine the implications of the identification of TMEM16A as it pertains to potential novel therapies in several pathologies.


Assuntos
Anoctamina-1/genética , Sinalização do Cálcio/genética , Canais de Cloreto/genética , Proteínas de Neoplasias/genética , Anoctamina-1/química , Cálcio/química , Agonistas dos Canais de Cálcio/química , Canais de Cloreto/química , Cloretos/química , Epitélio/química , Epitélio/metabolismo , Humanos , Proteínas de Neoplasias/química
14.
Eur J Histochem ; 62(1): 2836, 2018 02 16.
Artigo em Inglês | MEDLINE | ID: mdl-29569875

RESUMO

Human Merkel cells (MCs) were first described by Friedrich S. Merkel in 1875 and named "Tastzellen" (touch cells). Merkel cells are primarily localized in the basal layer of the epidermis and concentrated in touch-sensitive areas. In our previous work, we reported on the distribution of MCs in the human esophagus, so therefore we chose other parts of the human body to study them. We selected the human vagina, because it has a similar epithelium as the esophagus and plays very important roles in reproduction and sexual pleasure. Due to the fact that there are very few research studies focusing on the innervation of this region, we decided to investigate the occurrence of MCs in the anterior wall of the vagina. The aim of our research was to identify MCs in the stratified squamous non-keratinized epithelium of the human vagina in 20 patients. For the identification of Merkel cells by light microscopy, we used antibodies against simple-epithelial cytokeratins (especially anti-cytokeratin 20). We also tried to identify them using transmission electron microscopy. Our investigation confirmed that 10 (50 %) of 20 patients had increased number of predominantly intraepithelial CK20 positive "Merkel-like" cells (MLCs) in the human vaginal epithelium. Subepithelial CK20 positive MLCs were observed in only one patient (5%). We tried to identify them also using transmission electron microscopy. Our investigation detected some unique cells that may be MCs. The purpose of vaginal innervation is still unclear. There are no data available concerning the distribution of MCs in the human vagina, so it would be interesting to study the role of MCs in the vaginal epithelium, in the context of innervation and epithelial biology.


Assuntos
Epitélio/química , Células de Merkel/química , Vagina/química , Adulto , Idoso , Idoso de 80 Anos ou mais , Epitélio/ultraestrutura , Feminino , Humanos , Imuno-Histoquímica , Células de Merkel/ultraestrutura , Microscopia Eletrônica de Transmissão , Pessoa de Meia-Idade , Vagina/ultraestrutura
15.
Int J Parasitol Drugs Drug Resist ; 8(1): 145-157, 2018 04.
Artigo em Inglês | MEDLINE | ID: mdl-29571165

RESUMO

Haemonchus contortus, one of the most economically important parasites of small ruminants, has become resistant to the anthelmintic ivermectin. Deciphering the role of P-glycoproteins in ivermectin resistance is desirable for understanding and overcoming this resistance. In the model nematode, Caenorhabditis elegans, P-glycoprotein-13 is expressed in the amphids, important neuronal structures for ivermectin activity. We have focused on its ortholog in the parasite, Hco-Pgp-13. A 3D model of Hco-Pgp-13, presenting an open inward-facing conformation, has been constructed by homology with the Cel-Pgp-1 crystal structure. In silico docking calculations predicted high affinity binding of ivermectin and actinomycin D to the inner chamber of the protein. Following in vitro expression, we showed that ivermectin and actinomycin D modulated Hco-Pgp-13 ATPase activity with high affinity. Finally, we found in vivo Hco-Pgp-13 localization in epithelial, pharyngeal and neuronal tissues. Taken together, these data suggest a role for Hco-Pgp-13 in ivermectin transport, which could contribute to anthelmintic resistance.


Assuntos
Membro 1 da Subfamília B de Cassetes de Ligação de ATP/química , Membro 1 da Subfamília B de Cassetes de Ligação de ATP/genética , Membro 1 da Subfamília B de Cassetes de Ligação de ATP/metabolismo , Antiparasitários/metabolismo , Haemonchus/efeitos dos fármacos , Ivermectina/metabolismo , Homologia Estrutural de Proteína , Membro 1 da Subfamília B de Cassetes de Ligação de ATP/efeitos dos fármacos , Adenosina Trifosfatases/efeitos dos fármacos , Animais , Antiparasitários/administração & dosagem , Antiparasitários/farmacologia , Transporte Biológico , Caenorhabditis elegans/efeitos dos fármacos , Caenorhabditis elegans/parasitologia , Simulação por Computador , Dactinomicina/metabolismo , Resistência a Medicamentos/genética , Epitélio/química , Haemonchus/química , Haemonchus/genética , Ivermectina/administração & dosagem , Ivermectina/farmacologia , Simulação de Acoplamento Molecular , Faringe/química , Faringe/citologia , Ligação Proteica
16.
Zhonghua Bing Li Xue Za Zhi ; 47(3): 180-185, 2018 Mar 08.
Artigo em Chinês | MEDLINE | ID: mdl-29534357

RESUMO

Objective: To investigate the diagnostic value of some antibodies in peritoneal fluid of patients with gastric cancer and malignant epithelioid mesothelioma in serous effusion. Methods: One hundred and eighty-two cases of serous effusion were collected at Jilin Cancer Hospital, from July 2012 to July 2016. The expression of GLUT1, CDX2, Villin, calretinin and WT1 was evaluated using SP immunocytochemical technique in peritoneal fluid samples collected from 98 patients with gastric cancer and 74 patients with reactive mesothelial cells. The expression of GLUT1, calretinin and WT1 was also evaluated in serous effusion from 10 patients with mesothelioma. Results: The sensitivity of GLUT1, CDX2 and Villin in adenocarcinoma cells was 91.8%(90/98), 68.4% (67/98) and 88.8%(87/98), respectively. The specificity was 95.9% (71/74), 100.0%(74/74) and 100.0% (74/74), respectively. The sensitivity of calretinin and WT1 for reactive mesothelium was 93.2% (69/74) and 79.7% (59/74), respectively. The specificity was 96.9% (95/98) and 100.0% (98/98), respectively. The sensitivity of GLUT1, calretinin and WT1 for mesothelioma was 9/10, 9/10 and 7/10. The reactivity of GLUT1, CDX2, Villin, calretinin and WT1 showed a significant difference (P<0.01) between adenocarcinoma cells and reactive mesothelium. The reactivity of GLUT1 showed a significant difference (P<0.01) between mesothelioma and reactive mesothelium. Conclusions: The optimal combination is a panel of GLUT1, CDX2, Villin, calretinin and WT1 for differential diagnosis between adenocarcinoma cells and reactive mesothelium in peritoneal fluid of patients with gastric cancer. Whereas GLUT1, calretinin and WT1 is the best for differential diagnosis between reactive mesothelium and mesothelioma in serous effusions.


Assuntos
Adenocarcinoma/química , Líquido Ascítico/química , Neoplasias Pulmonares/química , Mesotelioma/química , Proteínas de Neoplasias/análise , Neoplasias Gástricas/química , Adenocarcinoma/diagnóstico , Biomarcadores Tumorais/análise , Fator de Transcrição CDX2/análise , Calbindina 2/análise , Diagnóstico Diferencial , Epitélio/química , Transportador de Glucose Tipo 1/análise , Humanos , Imuno-Histoquímica , Neoplasias Pulmonares/diagnóstico , Mesotelioma/diagnóstico , Proteínas dos Microfilamentos/análise , Sensibilidade e Especificidade , Neoplasias Gástricas/diagnóstico , Proteínas WT1/análise
17.
J Histochem Cytochem ; 66(2): 85-97, 2018 02.
Artigo em Inglês | MEDLINE | ID: mdl-29220632

RESUMO

Fibrinogen C domain containing 1 (FIBCD1) is a transmembrane receptor that binds chitin and other acetylated compounds with high affinity. FIBCD1 has previously been shown to be present in the epithelium of the gastrointestinal tract. In the present study, we performed a detailed analysis of normally structured human tissues for the expression of FIBCD1 by quantitative PCR and immunohistochemistry. We find that FIBCD1 is expressed in epithelial cells derived from all three germ layers. Endodermal-derived epithelial cells throughout the gastrointestinal tract and the respiratory system showed high expression of FIBCD1 and also mesodermal-derived cells in the genitourinary system and ectodermal-derived epidermis and sebaceous glands cells expressed FIBCD1. In some columnar epithelial cells, for example, in the salivary gland and gall bladder, the FIBCD1 expression was clearly polarized with strong apical reaction, while other columnar cells, for example, in small and large intestine and in bronchi, the staining was equally strong apically and basolaterally. In keratinocytes in skin, tongue, and oral cavity, the FIBCD1 staining was granular. This expression pattern together with the known binding properties supports that FIBCD1 plays a role in innate immunity in the skin and at mucosal surfaces.


Assuntos
Epitélio/química , Membrana Mucosa/química , Receptores de Superfície Celular/análise , Química Encefálica , Epitélio/metabolismo , Feminino , Trato Gastrointestinal/química , Trato Gastrointestinal/metabolismo , Expressão Gênica , Genitália Feminina/química , Genitália Feminina/metabolismo , Genitália Masculina/química , Genitália Masculina/metabolismo , Células HEK293 , Humanos , Imuno-Histoquímica , Masculino , Membrana Mucosa/metabolismo , RNA Mensageiro/análise , RNA Mensageiro/genética , Receptores de Superfície Celular/genética , Sistema Respiratório/química , Sistema Respiratório/metabolismo , Sistema Urinário/química , Sistema Urinário/metabolismo
18.
Pediatr Res ; 83(4): 791-797, 2018 04.
Artigo em Inglês | MEDLINE | ID: mdl-29281616

RESUMO

BackgroundIn children with congenital heart disease (CHD), altered pulmonary circulation compromises gas exchange. Moreover, pulmonary dysfunction is a complication of cardiac surgery with cardiopulmonary bypass (CPB). No data are available on the effect of different CHDs on lung injury. The aim of this study was to analyze epithelial lining fluid (ELF) surfactant composition in children with CHD.MethodsTracheal aspirates (TAs) from 72 CHD children (age 2.9 (0.4-5.7) months) were obtained before and after CPB. We measured ELF phospholipids, surfactant proteins A and B (SP-A, SP-B), albumin, and myeloperoxidase activity. TAs from 12 infants (age 1.0 (0.9-2.9) months) with normal heart/lung served as controls.ResultsHeart defects were transposition of great arteries (19), tetralogy of Fallot (TOF, 20), atrial/ventricular septal defect (ASD/VSD, 22), and hypoplastic left heart syndrome (11). Increased levels of ELF SP-B were found in all defects, increased myeloperoxidase activity in all except the TOF, and increased levels of ELF albumin and SP-A only in ASD/VSD patients. Postoperatively, ELF findings remained unchanged except for a further increase in myeloperoxidase activity.ConclusionELF composition has distinctive patterns in different CHD. We speculate that a better knowledge of the ELF biochemical changes may help to prevent respiratory complications.


Assuntos
Procedimentos Cirúrgicos Cardíacos/métodos , Ponte Cardiopulmonar , Cardiopatias Congênitas/patologia , Cardiopatias Congênitas/cirurgia , Ventrículos do Coração/fisiopatologia , Síndrome do Coração Esquerdo Hipoplásico/complicações , Circulação Pulmonar , Albuminas/química , Animais , Criança , Epitélio/química , Feminino , Comunicação Interventricular/fisiopatologia , Hemodinâmica , Heparina/química , Humanos , Lactente , Recém-Nascido , Pulmão/patologia , Lesão Pulmonar/fisiopatologia , Masculino , Modelos Animais , Peroxidase/química , Fosfolipídeos/química , Período Pós-Operatório , Alvéolos Pulmonares/patologia , Troca Gasosa Pulmonar , Surfactantes Pulmonares , Tensoativos/química , Tetralogia de Fallot/fisiopatologia , Traqueia/química , Transposição dos Grandes Vasos/fisiopatologia
19.
Allergy ; 73(3): 724-727, 2018 03.
Artigo em Inglês | MEDLINE | ID: mdl-29023780

RESUMO

Elements are vital in airway mucosal physiology and pathology, but their distribution and levels in the mucosa remain unclear. This study uses the state-of-the-art nuclear microscopy facility to map and quantify multiple elements in the histology sections of nasal mucosa from patients with nasal polyps or inverted papilloma. Our results demonstrate that P and Ca are the most abundant elements in mucosa and their distinct difference between epithelial and subepithelial regions; more importantly, our results reveal decreased amounts of Cu and Zn in the remodeled epithelium as compared to the normal epithelium. These findings suggest that Cu and Zn may be beneficial targets to regulate aberrant epithelial remodeling in airway inflammation.


Assuntos
Remodelação das Vias Aéreas , Epitélio/química , Mucosa Nasal/química , Adulto , Cálcio/análise , Cobre/análise , Humanos , Masculino , Pessoa de Meia-Idade , Microscopia Nuclear , Fósforo/análise , Zinco/análise
20.
J Biomed Mater Res B Appl Biomater ; 106(2): 716-725, 2018 02.
Artigo em Inglês | MEDLINE | ID: mdl-28323397

RESUMO

Biological surgical scaffolds are used in plastic and reconstructive surgery to support structural reinforcement and regeneration of soft tissue defects. Macrophage and fibroblast cell populations heavily regulate scaffold integration into host tissue following implantation. In the present study, the biological host response to a commercially available surgical scaffold (Meso BioMatrix Surgical Mesh (MBM)) was investigated for up to 9 weeks after subcutaneous implantation; this scaffold promoted superior cell migration and infiltration previously in in vitro studies relative to other commercially available scaffolds. Infiltrating macrophages and fibroblasts phenotypes were assessed for evidence of inflammation and remodeling. At week 1, macrophages were the dominant cell population, but fibroblasts were most abundant at subsequent time points. At week 4, the scaffold supported inflammation modulation as indicated by M1 to M2 macrophage polarization; the foreign body giant cell response resolved by week 9. Unexpectedly, a fibroblast subpopulation expressed macrophage phenotypic markers, following a similar trend in transitioning from a proinflammatory to anti-inflammatory phenotype. Also, α-smooth muscle actin-expressing myofibroblasts were abundant at weeks 4 and 9, mirroring collagen expression and remodeling activity. MBM supported physiologic responses observed during normal wound healing, including cellular infiltration, host tissue ingrowth, remodeling of matrix proteins, and immune modulation. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 716-725, 2018.


Assuntos
Epitélio/química , Teste de Materiais , Telas Cirúrgicas , Tecidos Suporte/química , Cicatrização , Animais , Feminino , Fibroblastos/metabolismo , Reação a Corpo Estranho/metabolismo , Células Gigantes de Corpo Estranho/metabolismo , Macrófagos/metabolismo , Camundongos
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