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1.
Vet Parasitol ; 277: 109017, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31901535

RESUMO

Cystic echinococcosis represents a significant problem in human and animal health and constitutes one of the most severe Neglected Tropical Diseases prioritized by the World Health Organization. The etiological agent is the complex Echinococcus granulosus sensu lato (s. l.), composed of several species/genotypes. Diagnosis in the definitive host and molecular epidemiology studies are important points for cystic echinococcosis control. Here we developed a new copro-LAMP assay, LAMP EGSL, for diagnosis in the definitive host for simultaneous detection of Echinococcus granulosus sensu stricto (s. s.), Echinococcus ortleppi, and Echinococcus canadensis species. Also, the analytical sensitivity, specificity and plausibility of performance in a rural context of a previously reported species-specific LAMP reaction, was evaluated. Both reactions showed high analytical sensitivity values (10 fg-100 fg DNA) and did not show cross reaction with DNA from host or other helminthic parasites. LAMP EGSL was performed with samples from an endemic area. In addition, the alkaline hydrolysis of one E. granulosus s. s. adult parasite followed by specific LAMP to E. granulosus s. s. was performed in a laboratory with low resources from another cystic echinococcosis endemic area. The results obtained suggest that LAMP EGSL represents a potential tool for canine diagnosis that could be useful for cystic echinococcosis control programs. In addition, we showed that LAMP reaction for E. granulous s. s., E. ortleppi and E. canadensis specific detection, could be useful for molecular epidemiology studies applicable to the definitive host. Both reactions were performed in endemic, rural areas without sophisticated equipment.


Assuntos
Testes Diagnósticos de Rotina/métodos , Doenças do Cão/diagnóstico , Equinococose/veterinária , Echinococcus granulosus , Parasitologia/métodos , Animais , Doenças do Cão/parasitologia , Cães , Equinococose/diagnóstico , Equinococose/parasitologia , Sensibilidade e Especificidade , Especificidade da Espécie
2.
Medicine (Baltimore) ; 98(51): e18141, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31860961

RESUMO

RATIONALE: Peliosis hepatis (PH), which is characterized by blood-filled cavities in the liver, is a rare disease. Its diagnosis depends on postoperative pathological examinations and immunohistochemistry. PATIENT CONCERNS: A 44-year-old female complained of right-middle upper abdominal pain and distension for 1 month, with occasional vomiting and fever. DIAGNOSIS: Because of the similar imaging features, the patient was initially misdiagnosed as cystic echinococcosis (CE). The immunoassay of echinococcosis was negative. Irregular hepatectomy was performed. Eventually, the patient was diagnosed with PH based on postoperative histopathology and immunohistochemistry. INTERVENTIONS: The patient underwent hepatectomy. Then, the cystic lesion was collected for intraoperative pathological examination. Thus, the blood liquid was extracted from the cystic lesion. Pringle maneuver was administered to prevent bleeding, and then the whole cystic lesion was removed. OUTCOMES: She recovered smoothly and there was no relapse occurred during 6 months' follow-up. LESSONS: It is difficult to differentiate PH from CE and other hepatic diseases due to the lack of special imaging features. Pathological examinations and immunohistochemistry can provide a confirmed diagnosis of PH.


Assuntos
Equinococose/diagnóstico , Hepatectomia/métodos , Peliose Hepática/diagnóstico , Peliose Hepática/patologia , Dor Abdominal/diagnóstico , Dor Abdominal/etiologia , Adulto , Biópsia por Agulha , Diagnóstico Diferencial , Equinococose/diagnóstico por imagem , Feminino , Seguimentos , Humanos , Imuno-Histoquímica , Imagem por Ressonância Magnética/métodos , Peliose Hepática/diagnóstico por imagem , Peliose Hepática/cirurgia , Doenças Raras , Índice de Gravidade de Doença , Tomografia Computadorizada por Raios X/métodos , Resultado do Tratamento
3.
J Helminthol ; 94: e100, 2019 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-31679536

RESUMO

Echinococcosis is a zoonotic parasitic illness that can cause significant disabilities, and even death for sick people. The disease is caused by the larval stage of cestodes belonging to the Echinococcus genus. In this study, multiple hydatid cysts were excised from an infected porcine liver. The identification of the parasitic species was made by the morphometric assessment of rostellar hooks and molecular detection of ribosomal DNA extant in protoscoleces of the hydatid sand. Rostellar hooks presented an average length of 27.4 µm by optical microscopy. Parasite DNA were detected in samples of hydatid sediment and positive controls by polymerase chain reaction. In conclusion, Echinococcus granulosus was recognized in samples of porcine hydatid cysts by microscopic observation, and the E. granulosus sensu lato strain E. canadensis G6/G7 was identified by molecular assay.


Assuntos
Equinococose/veterinária , Echinococcus granulosus/isolamento & purificação , Doenças dos Suínos/parasitologia , Animais , Equinococose/diagnóstico , Equinococose/parasitologia , Echinococcus granulosus/genética , Echinococcus granulosus/crescimento & desenvolvimento , Equador , Suínos , Doenças dos Suínos/diagnóstico
4.
Z Gastroenterol ; 57(11): 1281-1290, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31739373

RESUMO

INTRODUCTION: The prevalence of alveolar echinococcosis (AE) and cystic echinococcosis (CE) in Germany is increasing. The number of cases in northern and eastern regions is low, so there is little experience with regard to diagnosis and therapy. OBJECTIVES: The objective of this paper was to evaluate the management of suspected and/or diagnosed echinococcosis at a university center in a low-prevalence region. METHODS: All the patients at the Leipzig University Hospital between 2004 and 2018 who had been serologically examined for echinococci were included in a retrospective cohort study. Clinical course, imaging, histology, therapeutic characteristics, relevant comorbidities and risk factors for AE and CE were evaluated. A time-staggered prevalence estimation, as well as sensitivity and specificity calculations for the serological tests, were performed. RESULTS: A total of 382 patients were enrolled, with 11 AE and 7 CE cases identified. The mean prevalence rate of AE in this cohort was 2.9 % and that of CE was 1.8 %. Among the patients, 56 % had known risk factors for AE and CE. The serological tests showed a sensitivity of 86 % and a specificity of 91 %. Two patients with false-negative serology were diagnosed by biopsy. All CE and 5 AE patients (45 %) were operated on. Six AE patients received long-term treatment with albendazole. CONCLUSIONS: AE and CE are rare diseases in the greater Leipzig region; however, case numbers are on the rise. Due to favorable factors such as the escalation of migration, a further increase is expected. Diagnosis and therapy are challenging and should be supported by specialists (experienced infectiologists, imaging experts and skilled hepatobiliary surgeons) who should be integrated in a German network.


Assuntos
Equinococose Pulmonar/epidemiologia , Equinococose/epidemiologia , Echinococcus/isolamento & purificação , Albendazol/uso terapêutico , Animais , Anti-Helmínticos/uso terapêutico , Equinococose/diagnóstico , Equinococose/tratamento farmacológico , Equinococose/parasitologia , Equinococose Hepática/diagnóstico , Equinococose Hepática/tratamento farmacológico , Equinococose Hepática/epidemiologia , Equinococose Pulmonar/parasitologia , Alemanha/epidemiologia , Humanos , Doenças Negligenciadas/diagnóstico , Doenças Negligenciadas/tratamento farmacológico , Doenças Negligenciadas/epidemiologia , Prevalência , Estudos Retrospectivos
5.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi ; 31(4): 393-399, 2019 Sep 25.
Artigo em Chinês | MEDLINE | ID: mdl-31612674

RESUMO

OBJECTIVE: To explore the spatial and temporal distribution characteristics of cases with newly diagnosed echinococcosis in Sichuan Province from 2007 to 2017, so as to provide reference for the formulation of echinococcosis prevention and control strategies and for the identification of key areas. METHODS: The spatial distribution maps of detection of cases with newly diagnosed echinococcosis were plotted in Sichuan Province from 2007 to 2017, and the spatial distribution characteristics and epidemic trends were analyzed. RESULTS: From 2007 to 2017, the detection rate of cases with newly diagnosed echinococcosis appeared a decline in Sichuan Province year by year, and the areas with a high detection rate of cases with newly diagnosed echinococcosis were mainly located in western, northwestern and northern parts of Sichuan Province, while the areas with a low detection rate were predominantly found in the southern and eastern parts of the province. The global Moran's I values were 0.19, 0.22, 0.17, 0.44, 0.48, 0.31 and 0.16 for the detection rate of cases with newly diagnosed echinococcosis in Sichuan Province from 2010 to 2016 (all Z scores > 1.96, all P values < 0.05), suggesting spatial aggregation distribution during this period. Local spatial autocorrelation analysis revealed that the"high-high"areas and"low-low"areas for the detection rate of cases with newly diagnosed echinococcosis all showed an aggregation tendency. CONCLUSIONS: The detection rate of cases with newly diagnosed echinococcosis decreases in Sichuan Province from 2007 to 2017 year by year, and shows a spatial aggregation. The echinococcosis control activities should be intensified in Shiqu, Seda, Dege, Ganzi and Baiyu counties.


Assuntos
Equinococose , Análise Espaço-Temporal , China/epidemiologia , Equinococose/diagnóstico , Equinococose/epidemiologia , Equinococose/prevenção & controle , Epidemias , Humanos , Fatores de Tempo
6.
Turkiye Parazitol Derg ; 43(Suppl 1): 13-17, 2019 10 07.
Artigo em Inglês | MEDLINE | ID: mdl-31591873

RESUMO

Objective: Alveolar echinococcosis (AE) is one of the most lethal parasitic zoonoses in the Northern Hemisphere, and early serological detection is important to start treatment and to improve survival. A total of 50 sera samples of patients diagnosed as having various diseases were examined for by two different serological diagnostic methods. Methods: Em2-Em18 ELISA (Bordier Affinity Products, Crissier, Switzerland) and Echinococcus Western Blot immünoglobulin G (IgG) (LDBIO Diagnostics, Lyon, France) were used for analyisis. Results: A high titer of antibodies was found in 9 of 10 patients diagnosed as having AE with Em2-Em18 ELISA, in 2 of 21 patients with cystic echinococcosis, in 1 of 2 patients with fascioliasis and in 1 patient with chronic hepatitis. The Echinococcus Western Blot IgG test, used as a confirmatory test, showed IgG antibody in 85.7% (18/21) of patients with CE, while all serum samples of 10 patients with AE were evaluated as positive. This method yielded an incorrect diagnosis in the patient with chronic hepatitis and in the patient with granulomatous inflammation with caseification. Samples taken from patients with liver-related diseases and other parasitic-related diseases were found to be negative. Conclusion: The serological methods used in the study were found to be important in the early diagnosis of alveolar echinococcosis in the endemic areas, since it could be used in sero-epidemiological studies.


Assuntos
Anticorpos Anti-Helmínticos/sangue , Antígenos de Helmintos/imunologia , Equinococose/diagnóstico , Echinococcus/imunologia , Imunoglobulina G/sangue , Adulto , Animais , Western Blotting/métodos , Diagnóstico Diferencial , Equinococose Hepática/diagnóstico , Ensaio de Imunoadsorção Enzimática/métodos , Fasciolíase/diagnóstico , Feminino , Proteínas de Helminto/imunologia , Hepatite Crônica/diagnóstico , Humanos , Masculino
7.
Pan Afr Med J ; 33: 167, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31565128

RESUMO

Intra-orbital hydatid cyst is a very rare pathological entity that affects children and the young adults; it is secondary to the development in the orbit of the echinococcus granulosis tapeworm. Its frequency does not exceed 1% of all cases of hydatid disease. Clinical presentation of intra-orbital hydatid cyst is dominated by proptosis and a decrease in visual acuity, complete surgical excision is difficult, evolution is generally better when the treatment is early before the installation of irreversible optic atrophy. We report one case of a 3 years old girl operated for right intra-orbital hydatid cyst who presented with proptosis and blindness. Complete removal was difficult and puncture of the cyst was performed followed by excision of its membrane with good post-operative results. We also discuss the different epidemiological, clinical, radiological and therapeutic aspects of intra-orbital hydatid cyst and a review of literature of this rare pathology.


Assuntos
Equinococose/diagnóstico , Infecções Oculares Parasitárias/diagnóstico , Doenças Orbitárias/diagnóstico , Cegueira/parasitologia , Pré-Escolar , Equinococose/parasitologia , Equinococose/cirurgia , Exoftalmia/parasitologia , Infecções Oculares Parasitárias/parasitologia , Infecções Oculares Parasitárias/cirurgia , Feminino , Humanos , Doenças Orbitárias/parasitologia , Doenças Orbitárias/cirurgia , Acuidade Visual
9.
Artigo em Inglês | MEDLINE | ID: mdl-31497937

RESUMO

A 61-year-old male who lived for 30 years in a rural area presented chest pain of 3 months duration. Multiple hydatid cysts (Echinococcus granulosus) were diagnosed in the pericardium and the mediastinum by echocardiography and computed tomography. The cysts were removed successfully with the patient on cardiopulmonary bypass and beating heart.  This video tutorial shows how we removed the cysts, using the puncture-aspiration and enucleation technique.  Few videos of this technique exist, and we believe that this tutorial is a helpful demonstration of how to handle mediastinal and pericardial hydatid cysts.


Assuntos
Equinococose/cirurgia , Cardiopatias/cirurgia , Doenças do Mediastino/cirurgia , Procedimentos Cirúrgicos Torácicos/métodos , Equinococose/diagnóstico , Equinococose/parasitologia , Ecocardiografia , Cardiopatias/diagnóstico , Cardiopatias/parasitologia , Humanos , Masculino , Doenças do Mediastino/diagnóstico , Doenças do Mediastino/parasitologia , Mediastino , Pessoa de Meia-Idade , Pericárdio , Tomografia Computadorizada por Raios X
10.
Turkiye Parazitol Derg ; 43(3): 118-122, 2019 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-31502773

RESUMO

Objective: Echinococcus granulosus is the causative helminth of cystic echinococcosis (CE). The parasite is known to form fluid-filled cysts that grow slowly in the internal organs, particularly the liver and/or lungs. This disease is still important in terms of public health and economically in Turkey and other countries where animal husbandry is widespread. The aim of our study was to retrospectively evaluate the cases that were admitted to the Adnan Menderes University, Training and Research Hospital Parasitology laboratory on suspicion of CE between January 2005 and January 2017. Methods: Totally, 3446 sera (from 2019 female and 1427 male) were tested with an in-house ELISA for the presence of E. granulosus specific IgG antibodies at the timeswhen they were sent. Socio-demographic characteristics (age, gender, residence, and dog ownership), positivity titers, and cyst locations of pathologically confirmed CE patients were analyzed retrospectively. Results: The ages of patients varied between 4-87 years. It was found that 1104 (32%) of the 3446 sera were positive, and of them, 642 (58.1%) were female and 462 (41.9%) were male. Patients who had pathologically confirmed CE diagnosis constituted 247 (22.3%) of the total seropositive sera. Liver was the most commonly affected organ (81.8%), followed by lungs (6.1%). Conclusion: CE remains an important public health problem in our city; therefore, it is once again emphasized that preventive studies should be planned.


Assuntos
Equinococose/diagnóstico , Equinococose/epidemiologia , Echinococcus granulosus , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criação de Animais Domésticos , Animais , Anticorpos Anti-Helmínticos/sangue , Criança , Pré-Escolar , Equinococose/economia , Equinococose/parasitologia , Echinococcus granulosus/imunologia , Ensaio de Imunoadsorção Enzimática/métodos , Feminino , Hospitalização , Hospitais de Ensino , Hospitais Universitários , Humanos , Laboratórios Hospitalares , Fígado/parasitologia , Fígado/patologia , Pulmão/parasitologia , Pulmão/patologia , Masculino , Pessoa de Meia-Idade , Saúde Pública , Estudos Retrospectivos , Turquia , Adulto Jovem
11.
Turkiye Parazitol Derg ; 43(3): 149-151, 2019 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-31502806

RESUMO

The aim of the present study was to present a case with secondary hydatid cysts in both uterus and colon. The patient was a 71-year-old female living in Hakkari, Turkey. She was admitted to the Van Yuzuncu Yil University Faculty of Medicine Medical Center with complaints of chronic abdominal and pelvic pain, and swelling in the abdomen. First, the sagittal T2 weighted magnetic rezonance imaging (MR) showed a type-3 cyst hydatid with daughter vesicles located at the posterior of uterus. Later, MR revealed a type-2 cystic lesion with detached membrane adhered to the anterior wall of colon and it was reported to be associated with abdomen. When the previous liver surgery history of the patient was kept in mind, the new finding was suggestive of a secondary cystic hydatid . In conclusion, it is possible to diagnose secondary cystic echinococcosis in patients with a history of primary cyst surgery in liver or any other organ by combining the symptoms and imaging findings.


Assuntos
Colo/parasitologia , Equinococose/diagnóstico , Útero/parasitologia , Idoso , Animais , Colo/diagnóstico por imagem , Equinococose/diagnóstico por imagem , Equinococose/cirurgia , Equinococose Hepática/complicações , Equinococose Hepática/cirurgia , Echinococcus , Feminino , Humanos , Fígado/parasitologia , Fígado/cirurgia , Imagem por Ressonância Magnética , Recidiva , Turquia , Útero/diagnóstico por imagem
12.
Infect Dis Poverty ; 8(1): 68, 2019 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-31362789

RESUMO

BACKGROUND: Echinococcosis caused by Echinococcus is one of the most major infectious diseases in north-west highland of China. E. granulosus sensu strict, E. multilocularis, and E. canadensis are known to be the only three species related to human health transmitting in the areas. To achieve targeted treatment and control of echinococcosis, the accurate identification and discrimination of the species are important. However, currently the available diagnostic approaches do not present ideal results either in accuracy or efficiency. METHODS: In the study, a set of primers were designed to aim at the three human-pathogenic Echinococcus species in China. The one-step multiplex PCR assay was developed and evaluated for the specificity and sensitivity. A total of 73 parasitic lesions and 41 fecal materials obtained from human and various animals collected in the clinic and the field were tested to assess the applicability of this method. RESULTS: The multiplex PCR effectively detected the individual DNA from the targeted species and their random mixtures generating with distinguishable expected size of products. The detection limit of the assay for each of the three species was 5 pg/µl when they were tested separately. When DNA mixtures of the targeted species containing the same concentration were used as templates, the lowest amount of DNA which can be detected was 50 pg/µl, 10 pg/µl and 5 pg/µl for E. granulosus s. s., E. multilocularis, and E. canadensis respectively. No cross-reactivity was observed when DNA from eight genetically close species was used as control templates. The multiplex PCR identifications of all samples were in line with the original sequencing results except for those infected with E. shiquicus, which showed negative signals in the developed assay. Of all the tested stool materials, 16 were previously found positive for Echinococcus by visual and microscopic examination. Among these 16 samples, 13 were confirmed by the multiplex PCR, and the other three tested negative. Additionally, the multiplex PCR identified another 14 positive feces from the remained 25 stool samples which absence of worms. CONCLUSIONS: The developed multiplex PCR shows advantages in fast diagnosis and large-scale epidemiological investigation, which proven to be a promising tool utilized in clinic and surveillance system.


Assuntos
Equinococose/diagnóstico , Echinococcus/isolamento & purificação , Reação em Cadeia da Polimerase Multiplex/métodos , Animais , China , Diagnóstico Diferencial , Equinococose/classificação , Equinococose/parasitologia , Echinococcus granulosus/isolamento & purificação , Echinococcus multilocularis/isolamento & purificação , Humanos
13.
Khirurgiia (Mosk) ; (8): 79-84, 2019.
Artigo em Russo | MEDLINE | ID: mdl-31464280

RESUMO

Clinical symptoms and localization of echinococcosis are various. Clinical observation of successful surgical treatment of secondary multiple abdominal lesion with echinococcosis is presented in the article. Certain features of diagnosis and surgical treatment are described.


Assuntos
Cavidade Abdominal/parasitologia , Cavidade Abdominal/cirurgia , Equinococose/diagnóstico , Equinococose/cirurgia , Equinococose/complicações , Humanos
14.
Artigo em Inglês | MEDLINE | ID: mdl-31300133

RESUMO

Unawareness of canine parasitic diseases among at-risk hosts and an uncontrolled program of stray dog population have caused that zoonotic parasites received great attention in endemic regions of the Middle East. A total of 552 faecal samples were collected between December 2016 to January 2018 from stray (n = 408) and domestic (n = 144) dogs of Iran. All specimens were coproscopically observed following concentration and flotation techniques. Subsequently, the DNAs of taeniid eggs were extracted, amplified, and sequenced by targeting of mitochondrial cytochrome oxidase subunit 1 and small-subunit ribosomal DNA markers. The overall prevalence of canine intestinal parasites found 53.6%. The following parasites and their total frequencies were identified: taeniid (10.5%), Dicrocoelium dendriticum (0.7%), Trichuris vulpis (1.2%), Capillaria spp. (2.3%), Blastocystis spp. (5.2%), Ancylostoma spp. (2%), Eimeria spp. (13.2%), Dipylidium caninum (2.3%), Toxocara canis (3.8%), Giardia spp. (8.5%), and Toxascaris leonina (3.6%). Stray dogs were characterized more likely to be poliparasitized and indicated a higher prevalence of taeniid (10.9%), T. canis (4.4%) Giardia spp. (10.1%) than domestic dogs (P > 0.05). Phylogenetic and sequence analysis of Cox1 and SSU-rDNA indicated a low genetic diversity (Haplotype diversity; 0 to 0.495) in E. granulosus sensu lato G1, G3, G7 genotypes, and Taenia hydatigena. The pairwise sequence distances between G7 isolates showed an intra-diversity of 0.7%-1.5% and identity of 98.5%-100%. The first occurrence of pig strain (G7) from Iranian dogs might have substantial implications in the drug treatment of infected dogs due to the shorter maturation time of G7 compared with G1 genotype. Thus, the preventive strategies should be noticed to determine the risk factors, the importance of applying the hygienic practices, and well adjusting deworming programs for the Iranian dogs and at-risk individuals.


Assuntos
Doenças do Cão/epidemiologia , Equinococose/veterinária , Enteropatias Parasitárias/veterinária , Filogenia , Teníase/veterinária , Animais , Ciclo-Oxigenase 1/genética , DNA Ribossômico/genética , Doenças do Cão/diagnóstico , Cães/parasitologia , Equinococose/diagnóstico , Equinococose/epidemiologia , Echinococcus granulosus/genética , Fezes/parasitologia , Genótipo , Enteropatias Parasitárias/diagnóstico , Irã (Geográfico)/epidemiologia , Oriente Médio/epidemiologia , Mitocôndrias/genética , Prevalência , Suínos/parasitologia , Taenia/genética , Teníase/diagnóstico , Teníase/epidemiologia
15.
Chirurg ; 90(10): 823-832, 2019 Oct.
Artigo em Alemão | MEDLINE | ID: mdl-31312861

RESUMO

INTRODUCTION: Cystic and alveolar echinococcosis are two potentially life-threatening diseases caused by the helminthic parasites Echinococcus granulosus or Echinococcus multilocularis, respectively. In central Europe a marked increase in incidence of both forms of echinococcosis has recently been observed. OBJECTIVE: There is widespread uncertainty with diagnostic and treatment algorithms due to the close genetic relationship of both parasites and the similar presentation; however, since the treatment and prognosis significantly differ between the two diseases, a correct diagnosis is essential. This review summarizes the similarities and differences in the diagnostics and surgical treatment of these two parasites. RESULTS: The correct diagnosis is mainly based on patient history, radiological imaging and serological testing. In cystic echinococcosis cyst viability and stage determine whether treatment is medicinal, interventional, surgical or a watch and wait strategy. For alveolar echinococcosis surgical resection in combination with perioperative or adjuvant medicinal treatment is the gold standard and the only therapeutic option. As identification of the disease is often delayed due to the oligosymptomatic manifestation, complex resections are frequently necessary that need to be treated by a multidisciplinary team of experts. CONCLUSION: Echinococcosis is still a neglected disease due to the rarity and thus should be referred to experts in a tertiary hospital to avoid mistreatment or overtreatment. Due to a timely surgical, infectiological and interventional treatment in an interdisciplinary work-up, the prognosis of echinococcosis has significantly improved in recent years.


Assuntos
Equinococose , Echinococcus granulosus , Echinococcus multilocularis , Animais , Equinococose/diagnóstico , Equinococose/cirurgia , Europa (Continente) , Interações Hospedeiro-Parasita , Humanos , Incidência
16.
Biomed Res Int ; 2019: 4798906, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31360712

RESUMO

Cystic Echinococcosis (CE) is a widespread neglected zoonotic disease and is caused by the larval stage of the dog tapeworm Echinococcus granulosus sensu lato. CE is more frequent in livestock-rearing areas and where people live a nomadic or seminomadic lifestyle such as in Kajiado County, Kenya. There is limited data on CE disease situation in the county of Maasailand; the present study, therefore, reports on the prevalence of CE in cattle, sheep, and goats and their relative importance in CE transmission in Kajiado County. In total, 1,486 livestock (388 cattle, 625 sheep, and 473 goats) slaughtered in two abattoirs were examined for the presence of hydatid cysts in various organs. Cyst isolates were genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of the NADH dehydrogenase subunit 1 gene (nad1). The overall prevalence of CE was 14.8% (220/1486), while prevalence per livestock species was 15.2% (72/473) in goats, 14.9% (93/625) in sheep, and 14.2% (55/388) in cattle. Out of the 421 cysts isolated, 389 cysts were successfully characterized to be either E. granulosus sensu stricto (s. s.), 356/389 (91.5%), E. canadensis (G6/7), 26/389 (6.7%), or E. ortleppi, 7/389 (1.8%). This record confirms predominance of E. granulosus s. s. in Maasailand and other parts of Kenya, while the importance of E. ortleppi and E. canadensis (G6/7) to the general CE burden in Maasailand might be higher than previously thought. More so, a higher infection pressure for humans by E. granulosus s. s. based on its abundance could be speculated. The study sheds significant light on CE situation in livestock in the nomadic/seminomadic society of the Maasai in Kajiado County and provides good bases to investigate human CE in the area.


Assuntos
Equinococose , Echinococcus , Técnicas de Genotipagem , Proteínas de Helminto/genética , Helmintíase Animal , Gado/parasitologia , Animais , Bovinos , Cães , Equinococose/diagnóstico , Equinococose/epidemiologia , Equinococose/genética , Echinococcus/classificação , Echinococcus/genética , Cabras , Helmintíase Animal/diagnóstico , Helmintíase Animal/epidemiologia , Helmintíase Animal/genética , Humanos , Quênia/epidemiologia , Polimorfismo de Fragmento de Restrição , Ovinos
17.
Acta Trop ; 199: 105099, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31356785

RESUMO

Cystic Echinococcosis (CE) is a worldwide common helminth disease caused by the larval form of Echinococcus granulosus. The aim of this study is to determine the genetic differences between distinct isolates of E. granulosus obtained from cattle and sheep and determine the polymorphism of the AgB1 gene by DNA sequence analysis, as well as investigating its relationship with serological response using ELISA and Western Blot tests. For this aim, germinal membranes of hydatid cysts of 30 cattle and 30 sheep from the provinces of Elazig and Erzincan in Turkey and serum samples of these animals were collected. Following isolation of the total genomic DNA, the 12S rRNA gene of all isolates was amplified by PCR for genetic characterization, and the mt-CO1 gene region was examined by DNA sequence analysis. The gDNAs were then amplified by PCR using AgB1-specific primers, and genetic variation was investigated by DNA sequence analysis. At the final stage, all serum samples were analyzed by ELISA and Western Blot tests using a partially purified hydatid cyst fluid antigen. As a result, 114 (95%) of the 120 isolates were determined to be E. granulosus sensu stricto by using 12S rRNA-PCR. Subsequently, the DNA sequence analysis of the remaining 6 samples of the mt-CO1 gene revealed that all samples were E. granulosus sensu stricto. According to the DNA sequence analysis that followed, nucleotide changes in the AgB1 gene were observed in 13 (10.8%) of 120 samples. With this study, 9 (69.2%) out of 13 hydatid cysts in which polymorphism was detected by DNA sequence analysis in their AgB1 gene were found to be positive with ELISA, and 6 (46.1%) were found positive by WB. While 80 (74.7%) of 107 non-polymorphic samples in the AgB1 gene were found to be positive with ELISA, and 75 (70.9%) were positive with WB. As a result, variation in different ratios was determined in the AgB1 gene of E. granulosus sensu stricto, and it was determined that this had a partial effect on serological response.


Assuntos
Bovinos/parasitologia , Echinococcus granulosus/genética , Proteínas de Helminto/genética , Lipoproteínas/genética , Polimorfismo Genético , Ovinos/parasitologia , Animais , Equinococose/diagnóstico , Echinococcus granulosus/imunologia , Testes Sorológicos
18.
Parasit Vectors ; 12(1): 323, 2019 Jun 26.
Artigo em Inglês | MEDLINE | ID: mdl-31242932

RESUMO

BACKGROUND: Echinococcoses, caused by metacestodes of Echinococcus granulosus (cystic echinococcosis; CE) and E. multilocularis (alveolar echinococcosis; AE), represent major emerging parasitic diseases. These enzootic helminthiases invoke significant public health concerns and social burdens in endemic areas. The diseases are prevalent in the Qinghai-Tibetan Plateau, China, while community-based epidemiological studies have been scarcely reported. We surveyed echinococcosis patients in the southeastern Qinghai Province, China, to better understand the concurrent epidemiological situation in this area. METHODS: During July and August of 2013 and 2014, we screened echinococcosis patients at Yushu and Golog Prefectures, Qinghai Province, China, in a diagnostic campaign. A total of 2856 people (male:female ratio, 1:1.12; mean age, 34.6 years; age range, 6-88 years) were ultrasonographically examined for the presence of hepatic echinococcal cysts. We also collected serum samples from patients and analyzed antibody reactivity against recombinant forms of diverse E. granulosus antigen Bs (rEgAgB1-5) by enzyme-linked immunosorbent assay. RESULTS: We detected 134 patients whose imaging scans were compatible with CE (115 cases) and AE (20 patients). One patient might have been infected with both CE and AE. The overall incidence was 4.7% (CE, 4.0%; AE, 0.7%). A large proportion (67.5%) of CE patients was diagnosed at active and transitional CE1-CE3 stages in their late 30s. The AE cases were generally detected at advanced stage in patients at early 20s (60%). Analysis of the receiver operating characteristic curve and Youden's index indicated that rEgAgB2 was the most promising biomarker, followed by rEgAgB3 and rEgAgB1. Overall, sensitivity and specificity of rEgAgB1-3 were 84.5-92.7% and 91.9-94.6%, respectively. rEgAgB4 and 5 showed low sensitivity with high cross-reactivity. CONCLUSIONS: Our results strongly suggest that disability-adjusted life years related to echinococcoses in Qinghai-Tibetan areas might be more serious than previously considered. Control and prevention strategy against CE and AE are highly required in these areas. In addition to ultrasonography, serological tests might provide supportive data. However, serological data should be carefully interpreted for differential diagnosis, especially in areas where both CE and AE are co-endemic.


Assuntos
Anticorpos Anti-Helmínticos/sangue , Equinococose/diagnóstico , Equinococose/epidemiologia , Echinococcus granulosus/isolamento & purificação , Lipoproteínas/imunologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Animais , Criança , China/epidemiologia , Echinococcus granulosus/genética , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Lipoproteínas/genética , Masculino , Pessoa de Meia-Idade , Isoformas de Proteínas , Sensibilidade e Especificidade , Testes Sorológicos , Adulto Jovem
19.
Turkiye Parazitol Derg ; 43(2): 89-91, 2019 Jun 17.
Artigo em Inglês | MEDLINE | ID: mdl-31204463

RESUMO

Hydatic cyst is a crucial and prevalent parasitic disease in the developing countries in the Mediterranean region. Its diagnosis is sometimes problematic because of non-specific complaints and unavailability of any positive results in a routine laboratory analysis. Isolated renal hydatid cysts are very rare. In this study, a primary left renal hydatid cyst which was found in a 12-year-old boy was presented. He was referred by another hospital to our department with a flank pain and cystic mass in left kidney. Indirect haemagglutination test (IHA) for Echinococcus was negative. Nephrectomy was performed with the diagnosis of renal cyst hydatic. Renal cyst hydatid may present with various clinical findings ranging from asymptomatic clinical course to total loss in renal function. It will be beneficial to consider a renal hydatid cyst in patients with blurred flank pain, even if IHA is negative.


Assuntos
Equinococose/diagnóstico , Nefropatias/parasitologia , Animais , Criança , Equinococose/diagnóstico por imagem , Equinococose/cirurgia , Echinococcus granulosus/isolamento & purificação , Testes de Hemaglutinação , Humanos , Nefropatias/diagnóstico , Nefropatias/diagnóstico por imagem , Nefropatias/cirurgia , Masculino , Nefrectomia , Tomografia Computadorizada por Raios X , Ultrassonografia
20.
Turkiye Parazitol Derg ; 43(2): 99-101, 2019 Jun 17.
Artigo em Inglês | MEDLINE | ID: mdl-31204466

RESUMO

Hydatid disease is a parasitic disease. Although the disease causes many organ involvement, intracranial involvement is rarely seen. Clinical findings vary according to the number, size, localization of the cyst and the immune status of the patient. We aimed to present a rare case with intracranial hydatid cyst. A 8-year-old male patient presented with headache and vomiting. Physical examination revealed papillary edema. Brain computerized tomography imaging revealed a 13x13 cm cystic formation in the brain. The patient's echinococcal indirect hemagglutination test result was positive. The patient was started on oral albendazole therapy for intracranial hydatid cyst and was operated by brain surgery. Pathologic examination of the cyst was compatible with hydatid cyst. Although cysts are detected in the liver and lungs, cysts may be seen in atypically located areas. It should be kept in mind in clinically suspected patients that the disease can develop in rare organs, especially in patients with endemic areas. In patients with hydatid disease, advanced radiological examinations such as lung X-ray, abdominal ultrasonography and brain magnetic resonance imaging and echocardiographic examination should be performed for other organ involvement.


Assuntos
Encéfalo/parasitologia , Equinococose/diagnóstico , Administração Oral , Albendazol/administração & dosagem , Albendazol/uso terapêutico , Animais , Anti-Helmínticos/administração & dosagem , Anti-Helmínticos/uso terapêutico , Encéfalo/diagnóstico por imagem , Encéfalo/patologia , Encéfalo/cirurgia , Criança , Equinococose/tratamento farmacológico , Equinococose/cirurgia , Echinococcus/isolamento & purificação , Cefaleia , Testes de Hemaglutinação , Humanos , Masculino , Tomografia Computadorizada por Raios X , Vômito
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