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1.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi ; 32(4): 340-344, 2020 Jul 02.
Artigo em Chinês | MEDLINE | ID: mdl-32935505

RESUMO

OBJECTIVE: To establish a nucleic acid assay for detection of Echinococcus granulosus based on recombinase-aided isothermal amplification (RAA) assay. METHODS: The 12S rRNA gene of E. granulosus was selected as the target gene, and the specific primers and fluorescent probes for RAA assay were designed, screened and synthesized to establish a fluorescent RAA assay for detection of E. granulosus. The sensitivity of the fluorescent RAA assay was evaluated using different copy numbers of target gene sequence-contained recombinant plasmids and various concentrations of E. granulosus genomic DNA as templates, and the specificity of the fluorescent RAA assay was evaluated using the genomic DNA from E. granulosus, E. multilocularis, Schistosoma japonicum, S. mansoni, Ancylostoma duodenale, Clonorchis sinensis, Taenia saginata, Spirometra mansoni and Taenia solium as templates. RESULTS: A fluorescent RAA assay was successfully established for detection of E. granulosus, which achieved specific amplification of E. granulosus genomic DNA within 20 min at 39 ℃. The lowest detection limit of the fluorescent RAA assay was 10 copies/µL of recombinant plasmids and 0.1 ng/µL E. granulosus genomic DNA, which exhibited a high sensitivity, and the fluorescent RAA assay was all negative for the genomic DNA from E. multilocularis, S. japonicum, S. mansoni, A. duodenale, C. sinensis, T. saginata, Spirometra mansoni and T. solium, which exhibited a high specificity. In addition, this fluorescent RAA assay successfully detected genomic DNA from E. granulosus cysts. CONCLUSIONS: A rapid, sensitive and specific fluorescent RAA assay is successfully established for nucleic acid detection of E. granulosus.


Assuntos
Equinococose , Echinococcus granulosus , Técnicas de Amplificação de Ácido Nucleico , Animais , Primers do DNA , Equinococose/diagnóstico , Echinococcus granulosus/genética , Recombinases , Sensibilidade e Especificidade
2.
Korean J Parasitol ; 58(4): 451-456, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32871639

RESUMO

This is a report of 2 cases of human hydatidosis caused by Echinococcus ortleppi in Vietnam. The patients were a 12-year-old male (case 1) having a cyst of 10.0×9.0 cm size in the lung and a 50-year-old female with a 3.0×3.3 cm-sized cyst in the heart. Eosinophilia was 33.7% in the male and 45.8% in the female patient. C-reactive protein was increased to 16.5 mg/L in the male and 18.2 mg/L in the female. Both patients were positive for ELISA at OD=2.5 and 3.1, respectively. Echinococcus protoscolices were collected from the cysts by amniocentesis and surgery. The protoscolices were identified as E. ortleppi by morphology and analysis of mitochondrial NADH dehydrogenase 1 (nad1) gene sequence. Both patients were cured by surgical resection of the hydatid cyst combined with albendazole medication. The E. ortleppi infection in lung is the second report, and the other in the heart is the first in Vietnam.


Assuntos
Equinococose/diagnóstico , Equinococose/parasitologia , Albendazol/uso terapêutico , Animais , Biomarcadores , Proteína C-Reativa , Criança , DNA de Helmintos/genética , DNA Mitocondrial/genética , Equinococose/cirurgia , Echinococcus/genética , Echinococcus/isolamento & purificação , Ensaio de Imunoadsorção Enzimática , Feminino , Cardiopatias , Humanos , Pneumopatias Parasitárias , Masculino , Pessoa de Meia-Idade , NADH Desidrogenase/genética , Análise de Sequência de DNA , Vietnã
4.
Niger J Clin Pract ; 23(7): 1008-1012, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32620733

RESUMO

Objectives: The relationship hydatid cyst (HC) is a parasitic disease that is endemic worldwide. Aims: To study the clinical findings and laboratory results of patients with hydatid disease. Materials and Methods: Total number of patients (n = 78) who underwent surgery for HC disease from 2000 to 2017 were retrospectively evaluated. The patients' demographic characteristics, reasons for admission into hospital, lesion location and size, laboratory results, and complications were recorded. Results: Of the HCs, 59% and 26.9% were located in the liver and lungs, respectively. The rate of multiple organ involvement was 10.3%. A total of 16 (20.5%) cases had ruptured HCs (7 livers, 7 lungs, 1 spleen, and 1 omentum). There was no significant difference in the rate of eosinophilia between patients with and without cyst rupture (P = 0.9). Indirect hemagglutination (IHA) tests yielded negative results in 38.9% of the patients; among them, 32.1% had ruptured HCs. A negative IHA test result was significantly associated with rupture (P = 0.046). No significant difference between rupture and cyst size or location was found. Conclusions: HC rupture was not correlated with cyst size. The sensitivity of serological tests and the blood eosinophil count was low, even in cases of ruptured HCs. The recurrence rate can be reduced by open surgery and medical treatments.


Assuntos
Cistos/cirurgia , Equinococose Pulmonar/cirurgia , Equinococose/diagnóstico , Esplenopatias/cirurgia , Criança , Cistos/complicações , Equinococose/cirurgia , Equinococose Pulmonar/diagnóstico , Feminino , Testes de Hemaglutinação , Humanos , Pulmão/diagnóstico por imagem , Pulmão/patologia , Masculino , Omento/diagnóstico por imagem , Omento/patologia , Recidiva , Estudos Retrospectivos , Ruptura , Ruptura Espontânea/complicações , Baço/diagnóstico por imagem , Baço/patologia , Esplenopatias/diagnóstico , Ruptura Esplênica/etiologia
6.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi ; 32(2): 168-173, 2020 Mar 13.
Artigo em Chinês | MEDLINE | ID: mdl-32458606

RESUMO

OBJECTIVE: To establish a rapid nucleic acid detection technique for identification of Echinococcus multilocularis based on the recombinase aided isothermal amplification assay (RAA) and assess its diagnostic efficiency. METHODS: The mitochondrial gene sequence of E. multilocularis (GenBank accession number: AB018440) was used as a target sequence. The primers were designed according to the RAA reaction principle and synthesized, and RAA was performed using the generated primers. E. multilocularis genomic DNA at various concentrations and the pMD19-T (Simple) vector containing various copies of the target gene fragment were amplified using RAA to evaluate its sensitivity for detection of E. multilocularis, and RAA was em- ployed to detect the genomic DNA of E. granulosus G1 genotype, Taenia saginata, T. asiatica, T. multiceps, Dipylidium caninum, Toxocara canis, Trichuris trichiura, Giardia lamblia, Fasciola hepatica, Paragonimus westermani, Fasciola gigantica and Clonorchis sinensis to evaluate its specificity. In addition, the optimized RAA was employed to detect nine tissue specimens of E. granulosus-infected animals, 3 fecal samples from E. granulosus-infected dogs and 2 fecal samples from field infected dogs to examine its reliability and feasibility. RESULTS: The established RAA was able to detect the specific target gene fragment of E. multilocularis within 40 min. The lowest detect limit of RAA was 10 pg if E. multilocularis genomic DNA served as a template. If the re- combinant plasmid was used as a template, the minimally detectable copy number of RAA was 104. In addition, RAA was nega- tive for the genomic DNA of E. granulosus G1 genotype, T. saginata, T. asiatica, T. multiceps, D. caninum, T. canis, T. trichiura, G. lamblia, F. hepatica, P. westermani, F. gigantica and C. sinensis. The established RAA was positive for detection of the tissue specimens of infected animals, and simulated and field dog stool samples. CONCLUSIONS: A rapid, sensitive and specific RAA is established, which shows promising values in identification of E. multilocularis and gene diagnosis of alveolar echinococcosis.


Assuntos
Doenças do Cão , Equinococose , Echinococcus multilocularis , Animais , Primers do DNA , Doenças do Cão/diagnóstico , Doenças do Cão/parasitologia , Cães , Equinococose/diagnóstico , Equinococose/parasitologia , Equinococose/veterinária , Echinococcus multilocularis/genética , Echinococcus multilocularis/isolamento & purificação , Fezes/parasitologia , Técnicas de Amplificação de Ácido Nucleico , Recombinases/metabolismo , Reprodutibilidade dos Testes , Sensibilidade e Especificidade
7.
Chirurgia (Bucur) ; 115(2): 261-266, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32369731

RESUMO

The pancreatic localization of the hydatid cyst is exceptional, even in countries where hydatid disease is considered endemic. We describe a female patient, 63 years old, with hydatid cyst of the pancreas. The diagnosis was difficult because the presentation was that of an epigastric cyst of unknown origin, with no suggestive radiological and ultrasonography features. We performed a laparoscopic exploration of abdominal cavity which revealed a retrogastric cystic mass developed from the pancreatic body. The surgical treatment consisted in puncture, evacuation of proligera, lavage with hypertonic serum, partial excision of the pericyst and drainage of the cavity. The postoperative course was uneventfull and the patient was released after 5 days. The follow-up at 6, 12 and 24 month did not show relapse. Through this observation and a review of the literature, we discuss the diagnostic and therapeutic difficulties of this rare localization of the hydatid cyst. Hydatid cyst is a rare cause of a cystic lesion of the pancreas, especially in endemic areas. Surgery still remains the most effective treatment option. Laparoscopic approach is feasible and secure but requires a trained team with experience in minimally invasive surgery.


Assuntos
Equinococose/cirurgia , Laparoscopia/métodos , Pancreatopatias/cirurgia , Animais , Equinococose/diagnóstico , Feminino , Humanos , Pessoa de Meia-Idade , Pancreatopatias/diagnóstico , Pancreatopatias/parasitologia
8.
Ceska Gynekol ; 85(1): 35-40, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32414283

RESUMO

OBJECTIVE: Describe the case of echinococcal disease in gynecological practice and point out the complications of its diagnosis. DESIGN: Case report. SETTING: Department of Obstetrics and Gynaecology, Faculty Hospital Trenčín, Slovakia. CASE REPORT: The case is presented in an immunosuppressed patient with peritoneal carcinomatosis, mild Ca 125 elevation and increased CRP presenting as a finding of gynecological etiology with histopathological outcome and conclusion of parasitic disease - echinococcosis (hydatidosis). CONCLUSION: In the differential diagnosis of peritoneal carcinomatosis and ascites, especially in immunosuppressed patients with a positive social (or epidemiological) history, the possibility of rare parasitic diseases such as echinococcosis, which resembles malignant tumors, should be considered.


Assuntos
Equinococose/diagnóstico , Adulto , Ascite/diagnóstico , Proteína C-Reativa , Antígeno Ca-125/sangue , Diagnóstico Diferencial , Equinococose/sangue , Equinococose/parasitologia , Feminino , Humanos , Fragmentos de Peptídeos/sangue , Neoplasias Peritoneais/diagnóstico , Eslováquia
9.
Cesk Patol ; 56(1): 32-34, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32393044

RESUMO

Echinococcus multilocularis causes an aggressive form of hydatidosis whose histomorphological picture is generally not well recognized. We report a case of 39-year-old women presenting with poorly circumscribed nodules in the right hepatic lobe. Owing to the clinical suspicion of focal nodular hyperplasia and hepatocellular adenoma, a core biopsy was performed. The histological findings of necrotic fibrous tissue infiltrated by narrow epithelial cords and small cysts containing cytokeratin positive material were in concordance with the diagnosis of cholangiocarcinoma. Subsequent examination of the surgically resected necrotic nodules with a vital tissue at the periphery corresponded to a reparative fibrosis accompanied by a striking ductular proliferation. Serological and molecular genetic work-up led to the diagnosis of Echinococcus multilocularis. The aim of this report is to point out the unusual histological features of the solid foci of alveolar hydatidosis, which consisted of necrotic fibrous tissue with ductular reaction. Such findings in a core biopsy may simulate regressively altered carcinoma.


Assuntos
Equinococose , Echinococcus multilocularis , Hiperplasia Nodular Focal do Fígado , Fígado , Adulto , Animais , Biópsia , Equinococose/diagnóstico , Echinococcus multilocularis/isolamento & purificação , Feminino , Hiperplasia Nodular Focal do Fígado/diagnóstico , Humanos , Fígado/parasitologia
10.
Am J Trop Med Hyg ; 103(1): 303-307, 2020 07.
Artigo em Inglês | MEDLINE | ID: mdl-32394885

RESUMO

The aim of the study was to evaluate the availability of different procedures, diagnostic tests, and treatments, as well as the procedures and techniques used in the management of cystic echinococcosis (CE) in Spain. This was a cross-sectional study performed from September to December 2018 in Spain. A survey directed to CE-treating clinicians was conducted to collect information regarding the center characteristics and the different protocols of management followed. Thirty-nine centers among 76 contacted centers participated in the survey, most of them belonging to the public health system and attending both adult and children. The median number of patients with CE attended during the last three years per center was 15. Percutaneous techniques were used only in seven centers, and surgery was the most frequently used therapeutic approach. Drugs and duration of treatment (both when administered exclusively or when combined with surgery/puncture, aspiration, injection, and reaspiration) were very variable depending on the centers. There is a high variability in the management of CE among Spanish centers. These results stress the importance of promoting the diffusion of existing knowledge, adapting the WHO recommendations to our setting, and referring patients to referral centers at a national level.


Assuntos
Equinococose/diagnóstico , Adulto , Fatores Etários , Criança , Estudos Transversais , Equinococose/epidemiologia , Equinococose/terapia , Emigrantes e Imigrantes/estatística & dados numéricos , Inquéritos Epidemiológicos , Humanos , Espanha/epidemiologia
11.
PLoS Negl Trop Dis ; 14(4): e0008148, 2020 04.
Artigo em Inglês | MEDLINE | ID: mdl-32282820

RESUMO

BACKGROUND: Echinococcosis is a life-threatening parasitic disease caused by Echinococcus spp. tapeworms with over one million people affected globally at any time. The Echinococcus spp. tapeworms in the human body release DNA to the circulatory system, which can be a biomarker for echinococcosis. Cell-free DNA (cfDNA) is widely used in medical research and has been applied in various clinical settings. As for echinococcosis, several PCR-based tests had been trialed to detect cell-free Echinococcus spp. DNA in plasma or serum, but the sensitivity was about 20% to 25%. Low sensitivity of PCR-based methods might be related to our limited understanding of the features of cell-free Echinococcus spp. DNA in plasma, including its concentration, fragment pattern and release source. In this study, we applied ultra-high-throughput sequencing to comprehensively investigate the characteristics of cell-free Echinococcus spp. DNA in plasma of echinococcosis patients. METHODOLOGY/PRINCIPAL FINDINGS: We collected plasma samples from 23 echinococcosis patients. Total plasma cfDNA was extracted and sequenced with a high-throughput sequencing platform. An average of 282 million read pairs were obtained for each plasma sample. Sequencing data were analyzed with bioinformatics workflow combined with Echinococcus spp. sequence database. After identification of cell-free Echinococcus spp. reads, we found that the cell-free Echinococcus spp. reads accounted for 1.8e-5 to 4.0e-9 of the total clean reads. Comparing fragment length distribution of cfDNA between Echinococcus spp. and humans showed that cell-free Echinococcus spp. DNA of cystic echinococcosis (CE) had a broad length range, while that of alveolar echinococcosis (AE) had an obvious peak at about 135 bp. We found that most of the cell-free Echinococcus spp. DNA reads were from the nuclear genome with an even distribution, which might indicate a random release pattern of cell-free Echinococcus spp. DNA. CONCLUSIONS/SIGNIFICANCE: With ultra-high-throughput sequencing technology, we analyzed the concentration, fragment length, release source, and other characteristics of cell-free Echinococcus spp. DNA in the plasma of echinococcosis patients. A better understanding of the characteristics of cell-free Echinococcus spp. DNA in plasma may facilitate their future application as a biomarker for diagnosis.


Assuntos
DNA de Protozoário/sangue , Equinococose/diagnóstico , Equinococose/parasitologia , Echinococcus/genética , Echinococcus/isolamento & purificação , Sequenciamento de Nucleotídeos em Larga Escala/métodos , Adolescente , Adulto , Animais , Sequência de Bases , Biomarcadores , Criança , DNA de Protozoário/isolamento & purificação , Feminino , Genoma de Protozoário , Humanos , Masculino , Pessoa de Meia-Idade , Plasma , Reação em Cadeia da Polimerase/métodos , Sensibilidade e Especificidade , Análise de Sequência de DNA , Adulto Jovem
12.
J Am Vet Med Assoc ; 256(9): 1041-1046, 2020 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-32301665

RESUMO

CASE DESCRIPTION: A 17-week-old 14.4-kg (31.7-lb) female German Shepherd Dog from Missouri with a history of multiple intermittent episodes of vomiting and diarrhea underwent exploratory celiotomy. CLINICAL FINDINGS: At the time of surgery, the dog was bright, alert, and responsive, with a tender abdomen and palpable mesenteric lymph nodes. Hematologic data revealed mild leukocytosis, mild hypoproteinemia, and mild hypoalbuminemia. Moderate petechiation of the jejunal serosa and prominent mesenteric lymph nodes, but no palpable obstructions, were found during surgery. Jejunal and lymph node biopsies were performed; histologic examination revealed multiple segments of adult cestodes up to 700 µm long in the jejunum. Segments had a scolex and contained approximately 30- to 35-µm-diameter ova, morphologically compatible with Echinococcus spp. Fecal flotation revealed numerous proglottids and ova similar to those recognized histologically. Results of PCR assays confirmed Echinococcus multilocularis of E4 haplotype (a European strain). TREATMENT AND OUTCOME: Praziquantel (5 mg/kg [2.3 mg/lb], SC, once) was administered after surgery; treatments after hospital discharge included praziquantel (10 mg/kg [4.5 mg/lb], PO, once). No proglottids or ova were observed by fecal flotation after the treatments. The dog remained healthy without gastrointestinal signs 1 year later. CLINICAL RELEVANCE: The dog of this report had no travel history outside the state of Missouri. To the authors' knowledge, this is the first report of intestinal E multilocularis infection in a pet dog in the contiguous United States and first detection of a European strain of E multilocularis in this country. Findings suggested possible establishment of a European strain of this zoonotic pathogen in the contiguous United States.


Assuntos
Doenças do Cão , Equinococose , Echinococcus multilocularis , Animais , Doenças do Cão/diagnóstico , Cães , Equinococose/diagnóstico , Equinococose/cirurgia , Equinococose/veterinária , Fezes , Feminino , Missouri/epidemiologia , Praziquantel
13.
PLoS Negl Trop Dis ; 14(3): e0008147, 2020 03.
Artigo em Inglês | MEDLINE | ID: mdl-32155159

RESUMO

BACKGROUND: Echinococcosis is a chronic zoonosis caused by tapeworms of the genus Echinococcus. Treatment of the disease is often expensive and complicated, sometimes requiring extensive surgery. Ultrasonographic imaging is currently the main technique for diagnosis, while immunological analysis provides additional information. Confirmation still needs pathological analysis. However, these diagnostic techniques generally detect infection in late stages of the disease. An accurate, early and non-invasive molecular diagnostic method is still unavailable. METHODOLOGY/PRINCIPAL FINDINGS: We sequenced the cell-free DNA (cfDNA) from plasma of echinococcosis patients and confirmed the presence of Echinococcus DNA. To improve detection sensitivity, we developed a method based on targeted next-generation sequencing of repeat regions. Simulation experiments demonstrate that the targeted sequencing is sensitive enough to detect as little as 0.1% of an Echinococcus genome in 1 mL of plasma. Results obtained using patient plasma shows that the Area Under the Curve (AUC) of the method is 0.862, with a detection sensitivity of 62.50% and specificity of 100%, corresponding to a Youden-index of 0.625. CONCLUSIONS/SIGNIFICANCE: This study provides evidence that hydatid cysts release cfDNA fragments into patient plasma. Using the repeat region targeted sequencing method, highly specific detection of Echinococcus infection was achieved. This study paves a new avenue for potential non-invasive screening and diagnosis of echinococcosis.


Assuntos
DNA de Helmintos/sangue , Equinococose/diagnóstico , Echinococcus/genética , Técnicas de Diagnóstico Molecular/métodos , Plasma/química , Sequências Repetitivas de Ácido Nucleico , Adulto , Animais , DNA de Helmintos/química , DNA de Helmintos/genética , DNA de Helmintos/isolamento & purificação , Feminino , Sequenciamento de Nucleotídeos em Larga Escala/métodos , Humanos , Masculino , Sensibilidade e Especificidade
14.
Parasit Vectors ; 13(1): 133, 2020 Mar 14.
Artigo em Inglês | MEDLINE | ID: mdl-32171321

RESUMO

BACKGROUND: Cystic echinococcosis (CE) is a worldwide parasitic zoonosis caused by infection of the larval stage of tapeworm Echinococcus granulosus. In human CE, the parasites develop and form cysts in internal organs. The differentiated cysts can be classified into five types based on WHO-IWGE standard CE1-5 representing different developmental stages. Infection with E. granulosus triggers hosts' humoral and cellular response, displaying elevated serum antibodies and Th1 and Th2 cytokines, which are presumed to be in association with the disease outcome. Identification of immunological markers for evaluation of disease progression has been a growing concern. However, the distinctive profile of cytokines and antibodies associated with the cyst progression has not been ascertained. METHODS: To better understand the interaction between host immune response and disease outcome, the present study followed-up four CE patients over three years by yearly measuring serum level of 27 cytokines, total IgG and isotypes, and ultrasound scanning, beginning in year 1 for all patients with CE1 and CE2 cysts before treatment and continued in year 2 with CE4 and in year 3 with CE3-CE5 post-treatment. RESULTS: Nine cytokines including Th1-type IL-2, Th17-type IL-17A, and inflammatory cytokines IL-1ß, IL-1Rα and TNF-α, chemokines IL-8, MIP-1α, MIP-1ß, and growth factor G-CSF were significantly elevated in patients with cyst type CE1, compared to the normal controls, and then declined to a normal level at CE4 and CE5. Examining the antibody production, we found that serum specific IgG was significantly increased in patients with active and transitional cysts, specifically the total IgG at CE1/CE3/CE4-CE5, IgG4 at CE1 and IgG1 at CE1/CE3 cyst status, in comparison with the normal controls, but showed no significant changes between the cyst stages. CONCLUSIONS: Our findings provide new information on the profile of multiplex cytokines and serum antibodies associated with cyst stages in cystic echinococcosis patients through a three-year follow-up, implying that further studies using an approach combining cyst-associated immune parameters may aid in identifying immunological markers for differentiation of disease progression.


Assuntos
Anticorpos Antiprotozoários/sangue , Cistos/imunologia , Citocinas/sangue , Equinococose/imunologia , Echinococcus granulosus/imunologia , Idoso , Animais , China , Progressão da Doença , Equinococose/diagnóstico , Equinococose/parasitologia , Equinococose/terapia , Fazendeiros , Feminino , Seguimentos , Humanos , Imunoglobulina G/sangue , Estágios do Ciclo de Vida/imunologia , Masculino , Pessoa de Meia-Idade , Ultrassonografia
16.
Ann Thorac Surg ; 110(4): 1333-1338, 2020 10.
Artigo em Inglês | MEDLINE | ID: mdl-32145201

RESUMO

BACKGROUND: Cardiac involvement is an uncommon presentation of hydatid disease. In this study, we aim to analyze the experience of surgical treatment of cardiac and great vessels echinococcosis in our cardiovascular and thoracic surgery department. METHODS: Through a 16-year period, from 2000 to 2015, 27 patients underwent surgery for cardiac and great vessels hydatid disease. The clinical, operative, and postoperative data were analyzed through this retrospective and descriptive study. RESULTS: Most of our patients came from a rural area. The most common symptom was chest pain. The diagnosis was mainly made by transthoracic echocardiography, which has shown the right ventricle as the most frequent location of the disease. All patients received surgical treatment under cardiopulmonary bypass, and only six surgeries were performed without cross-clamping the aorta. Inhospital mortality rate was 7.4%. CONCLUSIONS: Cardiac hydatidosis is a rare but potentially serious condition whose treatment is mainly surgical even for asymptomatic patients owing to its possible fatal complications. The surgery outcomes are usually satisfactory. Follow-up examinations are highly recommended to detect recurrences.


Assuntos
Infecções Cardiovasculares/parasitologia , Infecções Cardiovasculares/cirurgia , Equinococose/diagnóstico , Equinococose/cirurgia , Cardiopatias/parasitologia , Cardiopatias/cirurgia , Complicações Pós-Operatórias/epidemiologia , Adolescente , Adulto , Idoso , Ponte Cardiopulmonar , Infecções Cardiovasculares/diagnóstico , Equinococose/mortalidade , Ecocardiografia , Feminino , Cardiopatias/diagnóstico , Mortalidade Hospitalar , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Resultado do Tratamento , Adulto Jovem
17.
Lakartidningen ; 1172020 02 11.
Artigo em Sueco | MEDLINE | ID: mdl-32045004

RESUMO

Alveolar echinococcosis (AE) caused by the fox tapeworm Echinococcus multilocularis is a zoonosis presenting with focal liver lesions and has a poor prognosis without treatment. The disease is common in Central and Eastern Europe but has been highly unusual in Sweden. A suspicion of AE usually arises through radiology and the diagnosis may be confirmed by histology and/or serological antibody detection. AE is treated with radical surgery in combination with anti-helminthic drug therapy. During the last two years six cases of AE have been diagnosed in Sweden. In no case was AE suspected clinically before biopsy. A heightened awareness of AE is needed among Swedish physicians, including radiologists, surgeons and pathologists.


Assuntos
Equinococose Hepática , Equinococose , Echinococcus multilocularis , Animais , Equinococose/diagnóstico , Europa Oriental , Suécia
18.
Int J Infect Dis ; 92: 181-183, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31981765

RESUMO

Extrahepatic hydatid disease has been reported in several locations, including the pelvis, and it often poses a diagnostic challenge. There are very few reported cases of primary perineal ischio-anal fossa hydatid cysts. We present a case of a 46-year-old female who had complained of painless left perianal mass for 8 months. The first clinical and radiological diagnosis was a cystic lesion of the perianal region. However, surgical and pathological examination revealed the diagnosis of hydatid disease of ischio-anal fossa. The patient underwent surgical excision. At 1-year follow-up, the patient was disease-free. Hydatid disease of the perianal area, although rare, must be kept in the differential diagnosis of pathologies of any perianal mass or lump.


Assuntos
Equinococose/diagnóstico , Animais , Diagnóstico Diferencial , Equinococose/diagnóstico por imagem , Equinococose/cirurgia , Echinococcus , Feminino , Humanos , Pessoa de Meia-Idade , Radiografia
19.
Vet Parasitol ; 277: 109017, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31901535

RESUMO

Cystic echinococcosis represents a significant problem in human and animal health and constitutes one of the most severe Neglected Tropical Diseases prioritized by the World Health Organization. The etiological agent is the complex Echinococcus granulosus sensu lato (s. l.), composed of several species/genotypes. Diagnosis in the definitive host and molecular epidemiology studies are important points for cystic echinococcosis control. Here we developed a new copro-LAMP assay, LAMP EGSL, for diagnosis in the definitive host for simultaneous detection of Echinococcus granulosus sensu stricto (s. s.), Echinococcus ortleppi, and Echinococcus canadensis species. Also, the analytical sensitivity, specificity and plausibility of performance in a rural context of a previously reported species-specific LAMP reaction, was evaluated. Both reactions showed high analytical sensitivity values (10 fg-100 fg DNA) and did not show cross reaction with DNA from host or other helminthic parasites. LAMP EGSL was performed with samples from an endemic area. In addition, the alkaline hydrolysis of one E. granulosus s. s. adult parasite followed by specific LAMP to E. granulosus s. s. was performed in a laboratory with low resources from another cystic echinococcosis endemic area. The results obtained suggest that LAMP EGSL represents a potential tool for canine diagnosis that could be useful for cystic echinococcosis control programs. In addition, we showed that LAMP reaction for E. granulous s. s., E. ortleppi and E. canadensis specific detection, could be useful for molecular epidemiology studies applicable to the definitive host. Both reactions were performed in endemic, rural areas without sophisticated equipment.


Assuntos
Testes Diagnósticos de Rotina/métodos , Doenças do Cão/diagnóstico , Equinococose/veterinária , Echinococcus granulosus , Parasitologia/métodos , Animais , Doenças do Cão/parasitologia , Cães , Equinococose/diagnóstico , Equinococose/parasitologia , Sensibilidade e Especificidade , Especificidade da Espécie
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