Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 1.761
Filtrar
1.
Front Public Health ; 10: 972619, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36091563

RESUMO

Background: Alveolar echinococcosis (AE) is a parasitic zoonosis with high mortality and disability rates. Diverse clinical manifestations and mimicking of differential diagnoses such as tuberculosis and malignancy pose a diagnostic dilemma. With the rapid development of molecular diagnostic techniques in recent years, metagenomic next-generation sequencing (mNGS) has become an attractive approach for the etiological diagnosis of infectious diseases. Case presentation: we report a case of 51-year-old Chinese Tibetan male presented with 3-year low-back pain and 4-month discomfort in the right upper quadrant of the abdomen. He had been in good health. He was diagnosed with tuberculosis and was given anti-tuberculosis treatment a month prior to the visit, but the symptoms were not relieved. Abdominal computerized tomography (CT) revealed a hypodense lesion with uneven enhancement in the liver, and two ring-enhancing cystic lesions in the right abdominal wall. Lumbar spine enhanced MRI showed lesions of mixed density with uneven enhancement in the L1 vertebra and paraspinal tissue. The pathological results of the liver biopsy revealed parasitic infection and possibly echinococcosis. The metagenomic next-generation sequencing (mNGS) of the puncture fluid of abdominal cysts using Illumina X10 sequencer revealed 585 sequence reads matching Echinococcus multilocularis. Disseminated AE was diagnosed. Albendazole (400 mg, twice daily) was used, and the patient was in stable condition during follow-up. Conclusions: mNGS may be a useful tool for the diagnosis of AE. The case would help clinicians to improve their diagnostic skills.


Assuntos
Equinococose , Echinococcus multilocularis , Animais , Equinococose/diagnóstico , Equinococose/parasitologia , Equinococose/patologia , Echinococcus multilocularis/genética , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Masculino , Pessoa de Meia-Idade , Tomografia Computadorizada por Raios X
2.
Can Vet J ; 63(9): 962-966, 2022 09.
Artigo em Inglês | MEDLINE | ID: mdl-36060492

RESUMO

Objective: Molecular identification of small cestodes, morphologically consistent with Echinococcus multilocularis, recovered at necropsy from the gastrointestinal tract contents of a red fox, was accomplished by PCR using published species-specific n ad1 primers and methods. Animal: Red fox (Vulpes vulpes). Procedure: Small cestodes recovered from intestinal contents of a red fox trapped on Prince Edward Island in December 2020 (frozen at -20°C before being processed for parasite recovery in June 2021) were morphologically identified. Species identity confirmation and haplotyping of the cestodes were done via PCR and DNA sequencing of the n ad1, nad2, and cob genes. Results: Small cestodes morphologically consistent with E. multilocularis were detected in the gastrointestinal tract contents of a red fox trapped near Montague, PEI. The species identity was confirmed via PCR. Haplotyping revealed that they were of the European E1 haplotype. Conclusion: In Canada, E. multilocularis has been reported as far east as Québec, with most reports being in central and western provinces and territories. This is the first report of E. multilocularis infection in a canid host east of Ontario, Canada and illustrates the need for regular wildlife disease surveillance to enhance our understanding of emerging pathogens of veterinary and medical importance. Clinical Relevance: Echinococcus multilocularis is a highly pathogenic zoonotic cestode from the family Taeniidae that can cause alveolar echinococcosis (AE) when rodents, dogs, horses, pigs, non-human primates, or humans ingest its eggs. Alveolar echinococcosis is challenging to treat, and survival rates for untreated individuals are low.


Objectif: L'identification moléculaire de petits cestodes, morphologiquement compatibles avec Echinococcus multilocularis, récupérés à l'autopsie du contenu du tractus gastro-intestinal d'un renard roux, a été réalisée par PCR à l'aide d'amorces nad1 spécifiques à l'espèce et de méthodes publiées. Animal: Renard roux (Vulpes vulpes). Procédure: De petits cestodes récupérés du contenu intestinal d'un renard roux piégé à l'Île-du-Prince-Édouard en décembre 2020 (congelés à −20 °C avant d'être traités pour la récupération des parasites en juin 2021) ont été morphologiquement identifiés. La confirmation de l'identité des espèces et l'haplotypage des cestodes ont été effectués par PCR et séquençage de l'ADN des gènes nad1, nad2 et cob. Résultats: De petits cestodes morphologiquement compatibles avec E. multilocularis ont été détectés dans le contenu du tractus gastro-intestinal d'un renard roux piégé près de Montague, Î.-P.-É. L'identité de l'espèce a été confirmée par PCR. L'haplotypage a révélé qu'ils étaient de l'haplotype européen E1. Conclusion: Au Canada, E. multilocularis a été signalé aussi loin à l'est que le Québec, la plupart des signalements ayant été rapportés dans les provinces et territoires du centre et de l'ouest. Il s'agit du premier rapport d'infection à E. multilocularis chez un canidé hôte à l'est de l'Ontario, au Canada, et illustre la nécessité d'une surveillance régulière des maladies de la faune pour améliorer notre compréhension des agents pathogènes émergents d'importance vétérinaire et médicale. Pertinence clinique: Echinococcus multilocularis est un cestode zoonotique hautement pathogène de la famille des Taeniidae qui peut provoquer une échinococcose alvéolaire (EA) lorsque des rongeurs, des chiens, des chevaux, des porcs, des primates non humains ou des humains ingèrent ses oeufs. L'échinococcose alvéolaire est difficile à traiter et les taux de survie des personnes non traitées sont faibles.(Traduit par Dr Serge Messier).


Assuntos
Doenças do Cão , Equinococose , Echinococcus multilocularis , Doenças dos Cavalos , Doenças dos Suínos , Animais , Cães , Equinococose/epidemiologia , Equinococose/parasitologia , Equinococose/veterinária , Echinococcus multilocularis/genética , Raposas/parasitologia , Cavalos , Humanos , Ontário , Ilha do Príncipe Eduardo , Suínos
3.
Parasite Immunol ; 44(10): e12944, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-36054717

RESUMO

Cystic echinococcosis (CE) is one of the most important zoonotic diseases with a worldwide distribution. It is caused by the larval stage of the dog tapeworm "Echinococcus granulosussensu lato" and constitutes a major economic and public health problem in several countries. Protoscoleces are one component of this larval stage that can interact with both definitive and intermediate hosts. The aim of the present study was to investigate the potential role of using a radio-attenuated form of these protoscoleces for immunoprophylaxis against experimental murine echinococcosis. However, mice were immunized twice at 15-day intervals with gamma (γ) irradiated protoscoleces at doses of 0.4, 0.8, 1.2 and 1.4 kGy then challenged with the intact parasites. Macroscopic and histological analyses with cytokine measurements were performed in order to estimate the number and diameter of cysts, microscopic changes and cytokine profile. An improvement in protection against the challenge dose was observed with increasing dose, giving percentages of 47.7, 49, 55.23 and 70.6%, for the 0.4, 0.8, 1.2 and 1.4 kGy-groups respectively. These data suggest that immunization with radio-attenuated protoscoleces may induce satisfactory protective immunity by reducing successfully the formation of cysts, caused by challenge infection.


Assuntos
Cistos , Equinococose , Echinococcus granulosus , Echinococcus , Animais , Citocinas , Cães , Equinococose/parasitologia , Equinococose/prevenção & controle , Raios gama , Larva , Camundongos
4.
Exp Parasitol ; 241: 108360, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-35995249

RESUMO

The main goal of the current study was to evaluate the effectiveness of resveratrol (RESV) on protoscolices and hydatid cysts of Echinococcus granolosus. Echinococcus granolosus protoscolices and hydatid cyst were exposed to RPMI, DMSO, formalin, mebendazole, and different concentrations of RESV in vitro. Then, viability, GGT, and caspase-3 activity of protoscolices were evaluated using light microscopy, colorimetric, and enzymatic assay, respectively. Tissue changes and expression of caspase-3 apoptosis were analyzed on the hydatid cyst wall by histologic and immunohistochemistry methods. The cell toxicity effect of RESV was evaluated on mouse PBMCs by Annexin V-FITC assay. The RESV-treated protoscolices showed loss of viability, increased gamma-glutamyl transpeptidase, and caspase-3 activity with significant differences compared to all control groups (P < 0.05). Dose and time dependence of mortality, GGT, and caspase-3 enzymatic activity was confirmed in the protoscolices of Echinococcus granulosus treated by RESV. Also, the tissue changes and apoptosis were prominent in RESV-treated hydatid cyst layers; however, tissue changes were only time-dependent, and RESV concentration had no apparent effect on tissue. In cell toxicity evaluation, RESV is safe without any significant apoptosis induction from 31.5 to 250 µg/ml; however, it was significant at 350 and 500 µg/ml in PBMCs.


Assuntos
Equinococose , Echinococcus granulosus , Echinococcus , Animais , Caspase 3 , Equinococose/tratamento farmacológico , Equinococose/parasitologia , Camundongos , Resveratrol/farmacologia , Resveratrol/uso terapêutico
5.
Exp Parasitol ; 240: 108321, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-35787385

RESUMO

Immune cells are pivotal players in the immune responses against both parasitic infection and malignancies. Substantial evidence demonstrated that there may exist possible relationship between echinococcus granulus sensu lato (E. granulosus s.l.) infection and hepatocellular carcinoma (HCC) development. Thus, this study aimed to observe crucial roles of immune cells in the formation of subcutaneous lesions after transplanting HepG2 cell lines with or without E. granulosus s.l. protoscoleces (PSCs). HepG2 cell lines were subcutaneously injected into nude mice in the control group. In the co-transplantation group, HepG2 cells were subcutaneously co-injected with high dosage of E. granulosus s.l. PSCs. From the 25th day of transplantation, volume of subcutaneous lesions was measured every four days, which were removed at the 37th day for further studies. Basic pathological and functional changes were observed. Moreover, expression of Ki67, Bcl-2, Caspase3, α-smooth muscle actin (α-SMA), T cell markers (CD3, CD4, CD8), PD1/PD-L1, nature killer (NK) cell markers (CD16, CD56) were further detected by immunohistochemical staining and quantitative real-time polymerase chain reaction (qRT-PCR) analysis. Subcutaneous lesions were gradually increased in volume and there occurred pathologically heterogeneous tumor cells, which were more significant in the co-transplantation group. Compared to the control group, expression of proliferation markers Ki67 and Bcl-2 was at higher levels in the co-transplantation group. Reversely, apoptotic marker Caspase3 was highly detected in the control group, suggesting promoting effects of E. granulosus s.l. PSCs on HCC development. Interestingly, subcutaneous lesions of the co-transplantation group were more functional in synthesizing and storing glycogen. Collagen and α-SMA+ cells were also at higher levels in the co-transplantation group than those in the control group. Most importantly, co-transplantation of HepG2 cells with E. granulosus s.l. PSCs led to significant increase in the expression of T cell markers, PD1/PD-L1 and NK cells markers. E. granulosus s.l. may have promoting effects on HCC development, which was closely associated with the immune responses of T cells and NK cells.


Assuntos
Carcinoma Hepatocelular , Equinococose , Echinococcus granulosus , Neoplasias Hepáticas , Animais , Antígeno B7-H1 , Equinococose/parasitologia , Genótipo , Antígeno Ki-67 , Camundongos , Camundongos Nus , Proteínas Proto-Oncogênicas c-bcl-2
6.
Ann Parasitol ; 68(2): 241-246, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35809448

RESUMO

Cystic echinococcosis (CE) is a parasitic zoonosis caused by the larval stage of Echinococcus granulosus sensu lato with medical importance and economic effect. This study was carried out in Sulaimani province, Kurdistan Region, Iraq from October 2020 to November 2021. The study focused on the prevalence of cystic echinococcosis in slaughtered animals. The total prevalence was 8% (1324/16524), with a high rate in sheep (5.3%) and low rates in goats and cattle (1.9% and 0.76%, respectively). With respect to abattoirs involved in the study, the highest prevalence was detected in Kalar abattoir (16.8%) and the lowest in Sulaimani abattoir (4.4%). Among the infected animals, sheep in Kalar abattoir recorded the highest prevalence, while cattle in Sulaimani abattoir recorded the lowest prevalence (11.7% and 0.4%, respectively). Males of sheep, cattle, and female goats showed higher prevalence (66.7%, 76.2%, and 58.6%, respectively). Regarding organ involvements, males of cattle and sheep showed high liver involvement (81.25% and 72.2%, respectively), while females of goat showed the highest liver involvement (76%). Females of cattle and sheep showed high lung involvement (46.7% and 28.5%, respectively), but the males of goats showed the highest lung involvement (53.8%).


Assuntos
Doenças dos Bovinos , Equinococose , Echinococcus granulosus , Echinococcus , Doenças das Cabras , Doenças dos Ovinos , Animais , Bovinos , Doenças dos Bovinos/epidemiologia , Doenças dos Bovinos/parasitologia , Equinococose/epidemiologia , Equinococose/parasitologia , Equinococose/veterinária , Feminino , Doenças das Cabras/epidemiologia , Doenças das Cabras/parasitologia , Cabras , Iraque , Masculino , Prevalência , Ovinos , Doenças dos Ovinos/epidemiologia , Doenças dos Ovinos/parasitologia
7.
Emerg Infect Dis ; 28(8): 1597-1605, 2022 08.
Artigo em Inglês | MEDLINE | ID: mdl-35850154

RESUMO

Human alveolar echinococcosis is caused by the parasite Echinococcus multilocularis, and dog ownership has been identified as a risk factor. We sought to specify the factors of dog ownership underlying this risk by conducting a case-control study among dog owners in Germany. The analysis revealed an increased odds ratio of ≈7-fold for dog owners whose dogs roam unattended in fields, 13-fold for dog owners who feed their dogs organic waste daily, 4-fold for dog owners who take their dog to a veterinarian only in case of illness, and 10-fold for dog owners who have never been informed by a veterinarian about the risk for infection. The results highlight the risk for infection associated with various factors of dog ownership and the value of veterinarians informing owners about prevention.


Assuntos
Doenças do Cão , Equinococose , Echinococcus multilocularis , Animais , Estudos de Casos e Controles , Doenças do Cão/epidemiologia , Doenças do Cão/parasitologia , Doenças do Cão/prevenção & controle , Cães , Equinococose/epidemiologia , Equinococose/parasitologia , Equinococose/transmissão , Equinococose/veterinária , Alemanha/epidemiologia , Humanos , Propriedade , Animais de Estimação
8.
Exp Parasitol ; 240: 108339, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-35863520

RESUMO

Cystic echinococcosis (CE) is a zoonotic parasitic disease caused by the metacestode of Echinococcus granulosus sensu lato (s.l.). A large proportion of the patients are asymptomatic at the early and late stages of the disease. CE diagnosis is mainly based on imaging techniques. Laboratory diagnosis including antibody-antigen (recombinant or fusion recombinant) can be used for the diagnosis and follow up of CE and alveolar echinococcosis (AE), but need optimization and standardization. This study aimed to evaluate the efficacy of a recombinant B-EpC1 (rB-EpC1) fusion antigen comprising B1, B2, B4, and EpC1 antigens of E. granulosus using indirect ELISA in comparison with a commercial ELISA kit for the serodiagnosis of CE. The recombinant protein was expressed in the expression host, E. coli BL21, and purified. This recombinant antigen was then evaluated by indirect ELISA and compared to the commercial CE diagnostic kit (Vircell, Spain). The study samples included 124 human sera consisting of 62 sera of patients with CE, and 62 sera of individuals without clinical evidences of CE and specific anti-CE antibodies in routine indirect ELISA. The diagnostic sensitivity and specificity of the indirect rB-EpC1-ELISA test for detection of specific anti-hydatid cyst antibodies in human CE were 95.2% and 96.8%, respectively. Also, the diagnostic sensitivity and specificity of the commercial ELISA test were 96.8% in this study. Initial evaluation of the recombinant fusion antigen (B-EpC1) was promising for the detection of CE by ELISA in clinical settings. Standardization and evaluation of recombinant fusion protein require further studies.


Assuntos
Equinococose , Echinococcus granulosus , Animais , Anticorpos Anti-Helmínticos , Antígenos de Helmintos/genética , Equinococose/parasitologia , Echinococcus granulosus/genética , Ensaio de Imunoadsorção Enzimática/métodos , Escherichia coli , Humanos , Sensibilidade e Especificidade
9.
Acta Trop ; 234: 106616, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-35901920

RESUMO

Cystic echinococcosis is parasitic disease caused by the metacestodes belonging to the Echinococcus granulosus sensu lato (s.l.) species complex. Cystic echinococcosis is of considerable economic and public health importance. It is endemic in both livestock and humans in North African countries, including Algeria. The present study aimed to characterize E. granulosus s.l. genotypes in dromedary camels (Camelus dromedarius) from the extreme Sahara of Algeria, using recently developed mitochondrial genetic markers (NADH dehydrogenase subunit 2 and NADH dehydrogenase subunit 5) for reliable identification of different genotypes. A total of 75 Echinococcus cysts were collected from 49 dromedary camels, including 65 and 10 cysts from 45 and four camels originating from two slaughterhouses of Tindouf and Illizi provinces, respectively. E. granulosus sensu stricto (s.s.) G1 and G3 were identified in camels from both areas based on nad5 (649 bp) gene sequences, whereas E. granulosus s.l. G6 was identified in camels from Tindouf region based on concatenated nad5 and nad2 gene sequences (total 1336 bp). Identified samples clustered into 11 different haplotypes (ALG1-ALG11), including four haplotypes (ALG8-ALG11) for E. granulosus s.s. G1, one haplotype (ALG7) for E. granulosus s.s. G3, and six haplotypes (ALG1-ALG6) for E. granulosus s.l. G6. The present study provides valuable molecular data, including genotyping and haplotypic variability, on E. granulosus s.l. in dromedary camels from two regions in the extreme Sahara of Algeria. Future characterization of the G1, G3, and G6 samples based on sequencing of complete mitochondrial genomes would be of considerable significance for a more comprehensive understanding of molecular epidemiology of CE in Algeria.


Assuntos
Cistos , Equinococose , Echinococcus granulosus , Argélia/epidemiologia , Animais , Camelus/parasitologia , Equinococose/epidemiologia , Equinococose/parasitologia , Equinococose/veterinária , Echinococcus granulosus/genética , Marcadores Genéticos , Genótipo , Haplótipos , Humanos , Manosiltransferases/genética , NADH Desidrogenase/genética
10.
Gene ; 835: 146659, 2022 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-35680021

RESUMO

Glutathione transferases (GSTs) perform catalytic and non-catalytic activities, mostly involved in stress-response and cell detoxification. Helminth parasites express several GSTs of multiple classes that are involved in the neutralization of potentially harmful oxidants, and in the inactivation or removal of xenobiotics. Additionally, GSTs participate in immunomodulatory processes that facilitate the parasite establishment and survival within its host. In Echinococcus granulosus sensu lato (s.l.) -the cestode parasite responsible for cystic echinococcosis- only one Mu-class GST has been reported. In the present work, by using bioinformatic and proteomic approaches we searched for novel Mu-class GSTs potentially involved in the parasite oxidative-stress metabolism. In the genome of E. granulosus s.l., 6 GST-related sequences were found to constitute a strongly conserved phylogenetical clade with Mu-class members. Among them, 5 displayed conserved gene structure (exon/intron), as well as specific residues and motifs characteristic of Mu-class enzymes. By proteomic analysis, 3 Mu-GSTs were identified to be expressed in the protoscolex parasite stage, 2 of them being firstly described as Mu-class GSTs here. The existence of more than one productive Mu-GST gene expands the parasite xenobiotic phase II metabolism, which might have beneficial roles on E. granulosus s.l. ability to successfully infect its host.


Assuntos
Equinococose , Echinococcus granulosus , Parasitos , Animais , Equinococose/parasitologia , Echinococcus granulosus/genética , Glutationa Transferase/genética , Glutationa Transferase/metabolismo , Parasitos/metabolismo , Proteômica
11.
Front Cell Infect Microbiol ; 12: 901994, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35770070

RESUMO

Cystic echinococcosis (CE) is a zoonotic disease worldwide distributed, caused by the cestode Echinococcus granulosus sensu lato (E. granulosus), with an incidence rate of 50/100,000 person/year and a high prevalence in humans of 5-10%. Serology has variable sensitivity and specificity and low predictive values. Antigens used are from the hydatid fluid and recombinant antigens have not demonstrated superiority over hydatid fluid. A cell line called EGPE was obtained from E. granulosus sensu lato G1 strain from bovine liver. Serum from CE patients recognizes protein extracts from EGPE cells with higher sensitivity than protein extracts from hydatid fluid. In the present study, EGPE cell protein extracts and supernatants from cell colonies were eluted from a protein G affinity column performed with sera from 11 CE patients. LC-MS/MS proteomic analysis of the eluted proteins identified four E. granulosus histones: one histone H4 in the cell extract and supernatant, one histone H2A only in the cell extract, and two histones H2A only in the supernatant. This differential distribution of histones could reflect different parasite viability stages regarding their role in gene transcription and silencing and could interact with host cells. Bioinformatics tools characterized the linear and conformational epitopes involved in antibody recognition. The three-dimensional structure of each histone was obtained by molecular modeling and validated by molecular dynamics simulation and PCR confirmed the presence of the epitopes in the parasite genome. The three histones H2A were very different and had a less conserved sequence than the histone H4. Comparison of the histones of E. granulosus with those of other organisms showed exclusive regions for E. granulosus. Since histones play a role in the host-parasite relationship they could be good candidates to improve the predictive value of serology in CE.


Assuntos
Cistos , Equinococose , Echinococcus granulosus , Animais , Bovinos , Extratos Celulares , Cromatografia Líquida , Equinococose/epidemiologia , Equinococose/parasitologia , Echinococcus granulosus/genética , Epitopos de Linfócito B , Genótipo , Histonas , Humanos , Fígado , Hepatopatias , Proteômica , Espectrometria de Massas em Tandem
12.
Exp Parasitol ; 238: 108282, 2022 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-35636496

RESUMO

Larval stages of taeniid Echinococcus granulosus are the infective forms of cystic echinococcosis or hydatidosis, a worldwide zoonosis. The protoscolex that develops into the adult form in the definitive host is enveloped by a complex cellular syncytial tegument, where all metabolic interchange takes place. Little information is available as to the electrical activity of the parasite in this developmental stage. To gain insight into the electrical activity of the parasite at the larval stage, we conducted microelectrode impalements of bovine lung protoscoleces (PSCs) of Echinococcus granulosus in standard saline solution. We observed two distinct intra-parasitic potentials, a transient peak potential, and a stable second potential, most likely representing tegumental and intra-parasitic extracellular space electrical potential differences. These values changed on the developmental status of the parasite, its anatomical regions, or time course after harvesting. Changes in electrical potential differences of the parasite provide an accessible and valuable parameter for the study of transport mechanisms and potential targets for developing novel antiparasitic therapeutics.


Assuntos
Equinococose , Echinococcus granulosus , Animais , Bovinos , Equinococose/parasitologia , Equinococose/veterinária , Larva
13.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi ; 34(2): 194-199, 2022 Apr 08.
Artigo em Chinês | MEDLINE | ID: mdl-35537844

RESUMO

Echinococcosis is a serious zoonotic parasitic disease caused by infections with larval Echinococcus. The life cycle of Echinococcus involves a variety of animal hosts, including hoofed animals and rodents as intermediate hosts and carnivores as definitive hosts. The transmission of human echinococcosis is closely associated with the life cycle of E. granulosus and E. multilocularis among animal hosts in nature. This review summarizes the recent advances in the prevalence and influencing factors of E. granulosus and E. multilocularis infections in animal hosts, so as to provide insights into precision control of echinococcosis.


Assuntos
Equinococose , Echinococcus granulosus , Echinococcus multilocularis , Animais , Equinococose/epidemiologia , Equinococose/parasitologia , Echinococcus granulosus/genética , Echinococcus multilocularis/genética , Humanos , Prevalência , Zoonoses/epidemiologia , Zoonoses/parasitologia
14.
Turkiye Parazitol Derg ; 46(2): 129-132, 2022 05 23.
Artigo em Inglês | MEDLINE | ID: mdl-35604191

RESUMO

Objective: Cystic echinococcosis (CE) is a common zoonotic disease in the world, which is caused by the larval form of Echinococcus granulosus settling in various organs in both animals and humans. It is common in livestock breeding areas and rural areas. This study was conducted to determine anti-E. granulosus seropositivity in patients with clinical pre-diagnosis/suspected CE in Kafkas University Health Research and Application Hospital in the Eastern Anatolia region of Turkey. Methods: Study material; between January 2018 and December 2020, 498 blood samples were sent to the Parasitology Laboratory from patients with clinical pre-diagnosis of CE, who applied to Kafkas University Health Research and Application Hospital for three years. The obtained serum samples were analyzed by indirect hemagglutination method. Results: Anti-Echinococcus granulosus antibodies were detected in 74 (14.9%) of 498 patients with clinical pre-diagnosis of hydatid cyst. Of the positive cases, 53 (71.6%) were observed in women, and 21 (28.4%) in men (p<0.05). Anti-E. granulosus antibodies were most commonly detected in the 16-30 age group (32.9%) (p<0.05), and 19.3% in the 16-60 age group. Conclusion: Although the study data do not cover the entire Eastern Anatolia region of Turkey, it has been concluded that KE maintains its importance as a public health problem in the region.


Assuntos
Equinococose , Echinococcus granulosus , Echinococcus , Animais , Equinococose/diagnóstico , Equinococose/epidemiologia , Equinococose/parasitologia , Feminino , Hospitais , Humanos , Universidades
15.
Turkiye Parazitol Derg ; 46(2): 133-139, 2022 05 23.
Artigo em Inglês | MEDLINE | ID: mdl-35604192

RESUMO

Objective: Cystic echinococcosis (CE) is a parasitic disease that has been known for years in helminth diseases and it is important as human and animal health problem in many parts of the world and in our country due to economic losses. In this study, it was aimed to retrospectively evaluate the distribution of anti-E. granulosus-IgG antibodies in patients with pre-diagnosis of CE that referred to parasitology laboratory between January 2013-December 2018. Methods: Commercial kit was used for indirect hemaglutination (IHA), indirect fluorescent antibody test (IFAT) and Western blot (WB) methods using sera from patient samples was applied according to the kit proposal. In addition, patient materials for CAM, CSF and blood for which polymerase chain reaction (PCR)/QPCR tests were requested were examined. Results: Sera of the patients who were tested with at least one of the IHA, IFAT and WB methods or a combination of these methods, and 443 cases out of 2.283 cases were found to be E. granulosus seropositive. It was determined that 369 (62.03%) of 443 positive patients were female and 330 (37.97%) were male patients. Among these patients, 87 patients whose IFAT and/or IHA tests were negative were found to have positive results with the WB method. IFAT or IHA test results of 13 patients with negative WB tests were found to be positive. Four patients were identified with both tests positive but WB test results negative. In addition, 36 of 72 patients who underwent PCR/QPCR tests were found to be positive. Conclusion: As a result of a six-year retrospective screening, 22% of the cases were found to be positive, and it was concluded that the prevalence of CE is high and the use of a single test may be insufficient in the diagnosis of CE, therefore, test combinations will increase the sensitivity and reliability in reaching the correct diagnosis.


Assuntos
Equinococose , Echinococcus granulosus , Animais , Anticorpos Anti-Helmínticos , Equinococose/diagnóstico , Equinococose/epidemiologia , Equinococose/parasitologia , Ensaio de Imunoadsorção Enzimática , Docentes , Feminino , Testes de Hemaglutinação , Humanos , Masculino , Reprodutibilidade dos Testes , Estudos Retrospectivos , Universidades
16.
Microb Pathog ; 168: 105586, 2022 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-35605738

RESUMO

Cystic Echinococcosis (hydatidosis) is caused by the larval stage of Echinococcus spp. It is an animal-borne zoonotic parasitic disease with local epidemic and natural foci, which is very common in northwest China. In recent years, a considerable attention has been paid to the epidemic investigation of hydatid disease in humans and sheep, but there are few large-scale epidemic investigation and data analysis of bovine hydatid disease. We systematically reviewed and analyzed the prevalence of bovine hydatid disease (2000-2021) in China for the first time. Several databases including CNKI, Wanfang, VIP Chinese periodical database, Baidu Library, PubMed and ScienceDirect were used to search 57 articles and 72 sets of valid data about bovine hydatid disease in China from 2000 to 2021. We used the random effect model in META package of R software, and PAS for rate conversion. The subgroup analysis and univariate meta regression analysis were used to reveal the factors leading to the heterogeneity of the study. The total prevalence rate of bovine hydatid disease in China from 2000 to 2021 is estimated to be 17.27% (10898/63113). According to the analysis of sampling years, the lowest positive rate since 2016 is 7.54% (1503/19929). The highest prevalence rate of bovine hydatid disease is 53.93% (4340/8048). The infection rate of bovine liver accounted for the highest proportion of the total infections, 45.2% (2040/4507). We also assessed the effects of different geographical and climatic factors on the prevalence of bovine hydatid disease. The results showed that the prevalence rate of hydatid disease was higher in cold and humid areas. Although the infection rate of bovine hydatid disease has declined in recent years, it is still necessary to carry out long-term surveillance and control of hydatid disease, cut off the infection route and reduce the risk of infection in high-risk areas.


Assuntos
Doenças dos Bovinos , Equinococose , Epidemias , Animais , Bovinos , Doenças dos Bovinos/epidemiologia , Doenças dos Bovinos/parasitologia , China/epidemiologia , Equinococose/epidemiologia , Equinococose/parasitologia , Equinococose/veterinária , Prevalência , Ovinos
17.
Parasitol Res ; 121(7): 1993-2008, 2022 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-35511364

RESUMO

Echinococcosis is a worldwide zoonosis. The mechanism of the establishment, growth, and persistence of parasites in the host has not been fully understood. Exosomes are found to be a way of information exchange between parasites and hosts. They exist in various body fluids widely. There are few studies on host-derived exosomes and their miRNA expression profiles at different infection time points. In this study, BALB/c mice were intraperitoneally infected with protricercariae. Exosomes were extracted from plasma (0, 3, 9, and 20 weeks post infection), and the expression profiles of exosome miRNA in the peripheral blood of mice were determined using RNA-sequencing. Compared to the 0 week groups, 24, 35, and 22 differentially expressed miRNAs were detected in infected mouse at the three infection stages, respectively. The results showed that there were significant differences in the miRNAs of exosomes at different infection time points. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were used to annotate the different miRNAs. The results showed that the biological pathways of parasites changed significantly at different stages of infection, with many significant and abundant pathways involved in cell differentiation, inflammation, and immune response, such as MAPK signaling pathway, Th17 cell differentiation, Wnt signaling pathway, FoxO signaling pathway, Notch signaling pathway, etc. These results suggest that miRNA may be an important regulator of interactions between Echinococcus granulosus and host. The data provided here provide valuable information to increase understanding of the regulatory function of microRNAs in the host microenvironment and the mechanism of host-parasite interaction. This may help us to find targets for Echinococcus granulosus to escape host immune attack and control Echinococcus granulosus infection in the future.


Assuntos
Equinococose , Echinococcus granulosus , MicroRNAs , Animais , Equinococose/parasitologia , Echinococcus granulosus/fisiologia , Perfilação da Expressão Gênica , Sequenciamento de Nucleotídeos em Larga Escala , Camundongos , Camundongos Endogâmicos BALB C , MicroRNAs/genética , MicroRNAs/metabolismo
18.
Acta Parasitol ; 67(3): 1401-1406, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-35536425

RESUMO

BACKGROUND: Alveolar hydatid disease caused by the tapeworm Echinococcus multilocularis is a parasitic disease present in the northern hemisphere. Echinococcus multilocularis is a parasite of canid and felid carnivores as definitive hosts, and small mammals, particularly rodents as intermediate hosts. Other animal species and humans can be aberrant intermediate hosts for this parasite. It is known that besides acting as definitive hosts, domestic dogs can rarely become infected with the larval form of E. multilocularis and develop alveolar echinococcosis; however, a role of wild canids as aberrant intermediate hosts has not been documented until now. To the best of our knowledge the present paper provides the first description of alveolar hydatid disease in a golden jackal (Canis aureus). CASE PRESENTATION: Necropsy of the yearling female animal found a large, round, tumor-like mass, 20 cm in diameter, with a rough, multilobulated surface in the abdominal cavity, connected to the liver and omentum. On the cut surface this tumor-like lesion was multicystic, with a number of locular cavities filled with a clear yellowish to orange watery fluid and a large area of necrosis in the central part of the mass. Histopathology revealed multiple cystic spaces separated by fibrous sheaths and inflammatory cells-lymphocytes, plasma cells, neutrophil and eosinophil granulocytes. The cysts contained either pale, hyaline, eosinophilic laminar and occasionally amorphous, acellular, PAS-positive structures, or metacestodes with invaginated protoscolices. In several cysts round calcified bodies (calcareous corpuscles) were noted. Microscopic examination showed everted and inverted protoscolices which were attached to fragments of the brood capsule or free in hydatid fluid. By comparing consensus nucleotide sequence of 457 bp obtained by PCR reaction with sequences deposited in NCBI GenBank it is determined that it was 100% identical with E. multilocularis sequences under accession numbers MH259778.1, MH259776.1, AB668376.1, EU704124.1 and AB018440.2. CONCLUSIONS: The present paper provides a proof that the golden jackal, besides being a definitive host, can also serve as the aberrant intermediate host for E. multilocularis.


Assuntos
Cistos , Equinococose , Echinococcus multilocularis , Animais , Cães , Equinococose/parasitologia , Equinococose/veterinária , Feminino , Humanos , Chacais/parasitologia
19.
Trends Parasitol ; 38(7): 501-512, 2022 07.
Artigo em Inglês | MEDLINE | ID: mdl-35508436

RESUMO

Zoonotic cestodes of the genus Echinococcus show marked differences in their distribution patterns which have not been satisfactorily explained. Echinococcus multilocularis is limited to the Holarctic, whereas species in the Echinococcus granulosus sensu lato (s.l.) complex mostly have a worldwide distribution. We proposed and tested a 'bridge effect' hypothesis stating that the low virulence of species in the E. granulosus s.l. complex for their intermediate hosts and a longer lifespan of infected hosts explain the differential distributions, particularly in hot and dry regions. This does not exclude the contribution of other factors such as human-mediated dispersal and intermediate host distribution. In the light of globalization and climate change, understanding what drives the distribution of zoonotic parasites is critical for preventing outbreaks of disease caused by these pathogens.


Assuntos
Equinococose , Echinococcus granulosus , Echinococcus multilocularis , Animais , Equinococose/parasitologia , Genótipo , Humanos
20.
Vet Parasitol Reg Stud Reports ; 30: 100724, 2022 05.
Artigo em Inglês | MEDLINE | ID: mdl-35431062

RESUMO

Alveolar echinococcosis is a severe, potentially fatal, parasitic disease caused by ingestion of microscopic eggs of Echinococcus multilocularis. The lifecycle of the parasite is essentially sylvatic, and based on a prey-predator relationship between red foxes and small rodents. A westward expansion from the eastern historical focus has been reported in France, though the parasite has also been detected in the southern Alps. While the focus in the Auvergne region (central France) was described in the 1980s, the southern delimitation of the actual endemic area, especially in the south, was unknown in the absence of dedicated surveys. Red fox samples were collected from 2013 to 2020 in the framework of other transversal epidemiological studies in five sampling areas from southwestern and southeastern France. One hundred and seven intestines were analysed by SSCT, and 221 faecal samples from intestines were analysed by copro-qPCR. None of the 328 foxes exhibited E. multilocularis worms or DNA. Although the presence of E. multilocularis cannot be totally excluded in the departments from the study areas, the sample size tested argues for an absence of the parasite in these studied areas, which is in accordance with the currently known endemic situation in France. These new data are helpful in determining the southernmost limit of E. multilocularis distribution in France. The warm, dry Mediterranean climate in the southeastern areas is less favourable to the transmission of E. multilocularis and especially to the survival of eggs in the environment than the climate in the French Alps or Liguria (Italy) climate where the parasite is present. The intermediate area between the southwestern study areas and the historical focus of Auvergne, which is separated by around 150 km, will be investigated in the coming years. Moreover, an ongoing national surveillance programme on E. multilocularis in foxes is targeting French departements along the edge of the known endemic area both in the southeast and southwest. The data produced will supplement the results of this study, thus greatly helping to define the current distribution of E. multilocularis in France and to target prevention measures to reduce human exposure.


Assuntos
Equinococose , Echinococcus multilocularis , Parasitos , Animais , Equinococose/epidemiologia , Equinococose/parasitologia , Equinococose/veterinária , Raposas/parasitologia , França/epidemiologia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...