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1.
Biosci Biotechnol Biochem ; 84(1): 31-36, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31794330

RESUMO

4-(2-Hydroxyphenethyl)-2,6-dimethoxyphenol, a bibenzyl, was isolated from the leaves of Empetrum nigrum var. japonicum, collected from Mount Tateyama. Japanese rock ptarmigans frequently eat the leaves and fruits of this plant. The structure of the bibenzyl was confirmed by NMR spectroscopic analysis and fully characterized. A synthesis of this compound was accomplished by coupling 2-hydroxyphenylacetic acid with syringaldehyde, decarboxylation of the resultant isoaurones, and hydrogenation of the double bond in the corresponding stilbene. This compound displayed cytotoxic activity against human cancer cells (HCT116 and Hela cells) and leukemia cells (HL-60 cells). The present study suggests that this plant serves as a source of biologically active natural products. Also, our findings provide information on the secondary metabolites in the diet of Japanese rock ptarmigans.


Assuntos
Bibenzilas/síntese química , Bibenzilas/farmacologia , Ericaceae/química , Extratos Vegetais/síntese química , Extratos Vegetais/farmacologia , Bacillus subtilis/efeitos dos fármacos , Bibenzilas/química , Bibenzilas/isolamento & purificação , Sobrevivência Celular/efeitos dos fármacos , Escherichia coli/efeitos dos fármacos , Células HCT116 , Células HL-60 , Células HeLa , Humanos , Japão , Espectroscopia de Ressonância Magnética , Conformação Molecular , Neoplasias/metabolismo , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Folhas de Planta/química , Pirogalol/análogos & derivados , Pirogalol/química
2.
Molecules ; 24(11)2019 May 29.
Artigo em Inglês | MEDLINE | ID: mdl-31146359

RESUMO

Some species of the Ericaceae family have been intensively studied because of the beneficial health impact, known since ancient times, of their chemical components. Since most studies focus on the effects of fruit consumption, this review aims to highlight the phenolic components present in the leaves. For this purpose, five species from Ericaceae family (bilberry-Vaccinium myrtillus L., lingonberry-V. vitis-idaea L., bog bilberry-V. uliginosum L., blueberry-V. corymbosum L. and bearberry-Arctostapylos uva-ursi L.) were considered, four of which can be found in spontaneous flora. The chemical composition of the leaves revealed three major phenolic compounds: chlorogenic acid, quercetin and arbutin. The health promoting functions of these compounds, such as antioxidant and anti-inflammatory properties that could have preventive effects for cardiovascular disease, neurodegenerative disorders, cancer, and obesity, have been exemplified by both in vitro and in vivo studies in this review. Furthermore, the importance of bioaccessibility and bioavailability of the phenolic compounds have been summarized. The findings highlight the fact that leaves of some Ericaceae species deserve increased attention and should be studied more profoundly for their biological activities, especially those from spontaneous flora.


Assuntos
Ericaceae/química , Fenóis/química , Fenóis/farmacologia , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Folhas de Planta/química , Animais , Disponibilidade Biológica , Ericaceae/classificação , Humanos , Compostos Fitoquímicos/química , Compostos Fitoquímicos/farmacologia
3.
Int J Mol Sci ; 20(12)2019 Jun 22.
Artigo em Inglês | MEDLINE | ID: mdl-31234551

RESUMO

Experiments conducted in vitro and in vivo, as well as some preclinical trials for cancer therapeutics, support the antineoplastic properties of lectins. A screening of antitumoral activity on HT29 colon cancer cells, based on polypeptide characterization and specific lectin binding to HT29 cells membrane receptors, was performed in order to assess the bioactivities present in four Mediterranean plant species: Juniperus oxycedrus subsp. oxycedrus, Juniperus oxycedrus subsp. badia, Arbutus unedo and Corema album. Total leaf proteins from each species were evaluated with respect to cell viability and inhibitory activities on HT29 cells (cell migration, matrix metalloproteinase -MMP proteolytic activities). A discussion is presented on a possible mechanism justifying the specific binding of lectins to HT29 cell receptors. All species revealed the presence of proteins with affinity to HT29 cell glycosylated receptors, possibly explaining the differential antitumor activity exhibited by the two most promising species, Juniperus oxycedrus subsp. badia and Arbutus unedo.


Assuntos
Neoplasias do Colo/tratamento farmacológico , Lectinas de Plantas/farmacologia , Antineoplásicos/farmacologia , Antineoplásicos/uso terapêutico , Sobrevivência Celular , Ericaceae/química , Células HT29 , Humanos , Juniperus/química , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Extratos Vegetais/uso terapêutico , Folhas de Planta/química , Lectinas de Plantas/uso terapêutico
4.
J Ethnopharmacol ; 235: 385-391, 2019 May 10.
Artigo em Inglês | MEDLINE | ID: mdl-30742883

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Arbutus unedo L., (Ericaceae) is one of the most traditional plants commonly used to treat diabetes in people living in Eastern Morocco region particularly in Taza and Beni Mellal. AIM OF THE STUDY: The aim of the study was to find if there is a scientific support to the ethnopharmacological relevance use of Arbutus unedo L., roots bark (AU) to treat diabetes. MATERIALS AND METHODS: We studied the effects of crude aqueous extract of AU on intestinal glucose absorption using short-circuit current technique in vitro and oral glucose tolerance test in vivo. RESULTS: The aqueous extract of AU (10 µg/mL to 1 mg/mL) induced concentration-dependent inhibition of sodium-dependent glucose transport across isolated mouse jejunum. The maximal inhibition was obtained with 1 mg/mL, which exhibited more than 80% of the Phloridzin inhibition with an IC50 close to 216 µg/mL. A 6-week AU ingestion (2 g/(kg day)), improved oral glucose tolerance as efficiently as metformin (300 mg/(kg day)). Arbutus unedo L. and metformin also reduced body weight. CONCLUSIONS: Arbutus unedo L. roots bark aqueous extract directly inhibited the electrogenic intestinal absorption of glucose in vitro. In addition it improved oral glucose tolerance and lowered body weight in rats after chronic oral administration in vivo. These results add a scientific support to the ethnopharmacological relevance use of Arbutus unedo L. roots bark to treat diabetes.


Assuntos
Ericaceae/química , Glucose/metabolismo , Hipoglicemiantes/farmacologia , Extratos Vegetais/farmacologia , Animais , Relação Dose-Resposta a Droga , Feminino , Teste de Tolerância a Glucose , Hipoglicemiantes/administração & dosagem , Hipoglicemiantes/isolamento & purificação , Concentração Inibidora 50 , Absorção Intestinal/efeitos dos fármacos , Masculino , Medicina Tradicional , Metformina/farmacologia , Camundongos , Camundongos Endogâmicos C57BL , Marrocos , Raízes de Plantas , Ratos , Ratos Sprague-Dawley
5.
Eur J Med Chem ; 166: 400-416, 2019 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-30739823

RESUMO

Grayanane diterpenoids, possessing a unique 5/7/6/5 tetracyclic system, exist exclusively in plants of the Ericaceae family. Owing to their various skeletons, complex structures, and diverse bioactivities, grayanoids have been the topic of research in many phytochemical and pharmacological laboratories, offering opportunities for the development of new drugs with analgesic, anti-inflammatory, and protein tyrosine phosphatase 1B (PTP1B) properties. Recently, a number of new grayanane diterpenoids with unprecedented carbon skeletons have been obtained from plants of the Ericaceae family, and they exhibit diverse biological properties, such as agalgesic, antinociceptive, anticancer, antiviral, antifeedant, insecticidal, toxicity, and PTP1B. In this review, 162 new grayanoids with 14 carbon skeletons from the Ericaceae family over the past seven years (2012-October 2018) are discussed, including their occurrence and distribution, skeleton types, structural features, biological activities, and structure-activity relationships (SARs). Also, strategies for the structural elucidation are summarized to provide useful information for medicinal chemists in developing potent anticancer, antiviral, analgesic, anti-inflammatory, and novel PTP1B agents.


Assuntos
Diterpenos/química , Diterpenos/farmacologia , Ericaceae/química , Analgésicos/química , Analgésicos/farmacologia , Anti-Inflamatórios/química , Anti-Inflamatórios/farmacologia , Antivirais/química , Antivirais/farmacologia , Inibidores Enzimáticos/química , Inibidores Enzimáticos/farmacologia , Humanos
6.
Food Chem ; 275: 426-438, 2019 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-30724217

RESUMO

An extract from Arbutus unedo fruits, rich in anthocyanins, was studied as a natural colorant with bioactive properties (antioxidant, antimicrobial and cytotoxic). The aqueous stability of the extract was monitored using the anthocyanins' content as response (determined by HPLC-DAD) in function of time, temperature and pH. Aided by mechanistic/phenomenological models, the conditions that favours the stabilization of the extract were provided, highlighting the suitability of the colorant for pastry/bakery products. As a case study, the extract was incorporated into wafers and the changes on the nutritional profile, free sugars, fatty acids and antioxidant properties were monitored during 6 days of storage. The results provide information for: i) potential application of the rich extract in anthocyanins for producing a natural colorant with bioactive properties; and ii) shelf-life predictions. The extract incorporation did not cause changes in the nutritional components of wafers but added colorant and antioxidant properties.


Assuntos
Antocianinas/isolamento & purificação , Ericaceae/química , Corantes de Alimentos/química , Glucosídeos/isolamento & purificação , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Antocianinas/análise , Anti-Infecciosos/química , Anti-Infecciosos/farmacologia , Antioxidantes/análise , Antioxidantes/farmacologia , Cromatografia Líquida de Alta Pressão/métodos , Ácidos Graxos/análise , Frutas/química , Concentração de Íons de Hidrogênio , Testes de Sensibilidade Microbiana , Temperatura Ambiente
7.
Food Chem ; 272: 66-75, 2019 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-30309595

RESUMO

The composition of honey influences how beneficial it is to human health. This study evaluated the physiochemical properties and total phenolic content (TPC) of single vs. multi-floral Irish and selected international honeys, and whether properties varied according to hive location. Oilseed rape honey had the lowest TPC of Irish unifloral honeys. Heather honey had the highest TPC, similar to Manuka honey (Mean ±â€¯SD = 68.16 ±â€¯2.73 and 62.43 ±â€¯10.03 respectively), and the TPC of ivy honey was approximately half that of heather. Urban multi-floral honeys contained higher TPC (28.26 ±â€¯13.63) than rural honeys (20.32 ±â€¯11.54). Physiochemical properties varied according to floral origin, and whether hives were in urban or rural sites. Irish heather honey had similar physiochemical characteristics to Manuka honey. This first examination of Irish honey confirms that TPC and physiochemical properties vary with honey type and hive location, and suggests that Irish heather honey should be examined for potential health benefits.


Assuntos
Mel/análise , Fenóis/análise , Cor , Condutividade Elétrica , Ericaceae/química , Ericaceae/metabolismo , Flores/química , Flores/metabolismo , Fungos/isolamento & purificação , Mel/classificação , Mel/microbiologia , Concentração de Íons de Hidrogênio , Fenóis/química , Espectrofotometria , Açúcares/análise , Água/química , Leveduras/isolamento & purificação
8.
J Asian Nat Prod Res ; 21(6): 559-572, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30585522

RESUMO

Three new megastigmane glucosides (1-3) and two new monoterpenes (4-5), together with 14 related known compounds (6-19) were isolated from the twigs and leaves of Lyonia ovalifolia. The structures of the new compounds were determined by extensive MS, NMR, CD experiments and chemical methods. Compounds 2, 6, and 18 displayed potent antiviral activity against Coxsackie B3, with IC50 values between 6.4 and 14.6 µM. Additionally, compounds 6, 10, and 11 exhibited noteworthy anti-inflammatory activities, with inhibition rates ranging from 54.55% to 83.33% under the concentration of 10-5 M.


Assuntos
Cicloexanonas/química , Cicloexanonas/farmacologia , Ericaceae/química , Glucosídeos/química , Glucosídeos/farmacologia , Norisoprenoides/química , Norisoprenoides/farmacologia , Animais , Anti-Inflamatórios não Esteroides/química , Anti-Inflamatórios não Esteroides/farmacologia , Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/farmacologia , Antivirais/química , Antivirais/farmacologia , Enterovirus/efeitos dos fármacos , Lipopolissacarídeos/farmacologia , Camundongos , Estrutura Molecular , Monoterpenos/química , Monoterpenos/farmacologia , Óxido Nítrico/antagonistas & inibidores , Folhas de Planta , Células RAW 264.7 , Células Vero
9.
Fitoterapia ; 133: 29-34, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30572088

RESUMO

In our on-going study to investigate components with analgesic activity, eight new grayanane diterpenoids, epoxypieristoxins A-H (1-8), along with one known compound (9) were isolated from the roots of Pieris formosa. Their structures with absolute configurations were characterized by a series of spectroscopic methods and X-ray diffraction. Notably, compounds 1-5 represented the first example of natural grayanane diterpenoids possessed a 10,14-epoxy group. Whereas, compounds 6-7 were the first example of grayanane diterpenoid with a 7,10-epoxy group. Biological assays showed that compounds 1-3 and 5-8 displayed significant analgesic activity at a dose of 5.0 mg/kg (ip) compared to the vehicle tests (p < .05).


Assuntos
Analgésicos/farmacologia , Diterpenos/farmacologia , Ericaceae/química , Raízes de Plantas/química , Analgésicos/isolamento & purificação , Animais , Diterpenos/isolamento & purificação , Camundongos , Estrutura Molecular , Compostos Fitoquímicos/isolamento & purificação , Compostos Fitoquímicos/farmacologia
10.
Crit Rev Food Sci Nutr ; 59(6): 864-881, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30582347

RESUMO

In addition to nutrients, plant foods contain compounds that may provide additional health benefits improving the quality of life. Species from Arbutus genus (Ericaceae) represent a promising source of healthy phytochemicals. Bioactive compounds including such as anthocyanins, iridoids, phenols, triterpenes, sterols, and fatty acids are reported from Arbutus species. Some Arbutus species revealed promising biological activities including antioxidant, anti-inflammatory, anti-proliferative, anti-diabetic, and antimicrobial activities, and deserve for that reason further consideration for new drug discovery. However, only few species are investigated scientifically for their chemical profile and biological activities. The aim of this article is to summarize the current knowledge of the components and biological properties of Arbutus species common in Mediterranean area, as well as the future prospects on their applications as potentially valuable products.


Assuntos
Ericaceae/química , Compostos Fitoquímicos/análise , Compostos Fitoquímicos/farmacologia , Extratos Vegetais/análise , Extratos Vegetais/farmacologia , Animais , Anti-Infecciosos/análise , Anti-Infecciosos/farmacologia , Anti-Inflamatórios/análise , Anti-Inflamatórios/farmacologia , Antioxidantes/análise , Antioxidantes/farmacologia , Doenças Cardiovasculares/tratamento farmacológico , Linhagem Celular Tumoral , Frutas/química , Humanos , Hipoglicemiantes/análise , Hipoglicemiantes/farmacologia , Fenóis/análise , Fenóis/farmacologia , Fitosteróis/análise , Fitosteróis/farmacologia , Folhas de Planta/química
11.
Fitoterapia ; 131: 168-173, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30149098

RESUMO

Phytochemical investigation on the aerial parts of Lyonia ovalifolia (Wall.) Drude led to the isolation of three new iridoids, lyonofolin A (1), lyonofolin B (2), and lyonofolin C (3), and a known iridoid, gelsemiol (4). Structures of compounds 1-4 were determined by extensive spectroscopic analyses, including EI-MS, HREI-MS, UV, IR, and 1D- and 2D-NMR (HMBC, HSQC, COSY, NOESY) spectroscopic methods. The effect of insulin secretion of compounds 1, 2, and 4 were evaluated in mice pancreatic islets cellular model. This insulin secretory assay demonstrated that compound 2 potentiates glucose-induced insulin secretion, and thus can serve as a new insulin secretagogue for the treatment of diabetes. The newly isolated compounds were further evaluated against normal 3 T3 cell lines for cytotoxicity, where they did not show any cytotoxicity.


Assuntos
Ericaceae/química , Hipoglicemiantes/farmacologia , Iridoides/farmacologia , Ilhotas Pancreáticas/efeitos dos fármacos , Células 3T3 , Animais , Hipoglicemiantes/isolamento & purificação , Insulina/metabolismo , Iridoides/isolamento & purificação , Ilhotas Pancreáticas/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Estrutura Molecular , Nepal , Compostos Fitoquímicos/isolamento & purificação , Compostos Fitoquímicos/farmacologia , Componentes Aéreos da Planta/química , Extratos Vegetais/química
12.
Food Chem ; 264: 81-91, 2018 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-29853408

RESUMO

The goal of this study was to compare anthocyanin extraction from Arbutus unedo L. fruits, by using two techniques assisted by different energy sources, heat and ultrasound. In order to obtain the conditions that maximize anthocyanin extraction, a response surface methodology was applied using the circumscribed central composite design of three variables with five levels. Three anthocyanin compounds were identified by HPLC-DAD-ESI/MS, being cyanidin-3-glucoside the main molecule. Heat assisted extraction proved to be the most effective method at 5 min, 90 °C and 80% of ethanol, yielding 51.2% of extract, with a total anthocyanin content of 382.4 µg/g dried fruit, and 744.6 µg/g extract. These response values were slightly improved by studying the solid/liquid effect at the optimal conditions in dose-response format, showing steady extraction values from 5 to 40 g/L. Results show the possibility of using A. unedo fruits as a source of anthocyanin compounds for industrial applications.


Assuntos
Antocianinas/isolamento & purificação , Fracionamento Químico/métodos , Ericaceae/química , Antocianinas/análise , Cromatografia Líquida de Alta Pressão/métodos , Frutas/química , Glucosídeos/análise , Glucosídeos/isolamento & purificação , Temperatura Alta , Espectrometria de Massas por Ionização por Electrospray , Ultrassom
13.
Sci Rep ; 8(1): 6965, 2018 05 03.
Artigo em Inglês | MEDLINE | ID: mdl-29725038

RESUMO

Parkinson's disease (PD) is an age-related neurodegenerative disease associated with the misfolding and aggregation of alpha-synuclein (aSyn). The molecular underpinnings of PD are still obscure, but nutrition may play an important role in the prevention, onset, and disease progression. Dietary (poly)phenols revert and prevent age-related cognitive decline and neurodegeneration in model systems. However, only limited attempts were made to evaluate the impact of digestion on the bioactivities of (poly)phenols and determine their mechanisms of action. This constitutes a challenge for the development of (poly)phenol-based nutritional therapies. Here, we subjected (poly)phenols from Arbutus unedo to in vitro digestion and tested the products in cell models of PD based on the cytotoxicity of aSyn. The (poly)phenol-digested metabolites from A. unedo leaves (LPDMs) effectively counteracted aSyn and H2O2 toxicity in yeast and human cells, improving viability by reducing aSyn aggregation and inducing its clearance. In addition, LPDMs modulated pathways associated with aSyn toxicity, such as oxidative stress, endoplasmic reticulum (ER) stress, mitochondrial impairment, and SIR2 expression. Overall, LPDMs reduced aSyn toxicity, enhanced the efficiency of ER-associated protein degradation by the proteasome and autophagy, and reduced oxidative stress. In total, our study opens novel avenues for the exploitation of (poly)phenols in nutrition and health.


Assuntos
Polifenóis/farmacologia , Agregados Proteicos/efeitos dos fármacos , Proteostase/efeitos dos fármacos , alfa-Sinucleína/metabolismo , Autofagia/efeitos dos fármacos , Linhagem Celular , Ericaceae/química , Humanos , Peróxido de Hidrogênio/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Doença de Parkinson/metabolismo , Doença de Parkinson/terapia , Polifenóis/química
14.
Food Res Int ; 109: 455-471, 2018 07.
Artigo em Inglês | MEDLINE | ID: mdl-29803472

RESUMO

The aim of this work was to produce an extract rich in different bioactive compounds from Arbutus unedo L. fruits to enhance its possible valorization and commercialization. The conditions of the main variables of maceration, microwave, and ultrasound assisted extractions (MA, MAE and UAE, respectively) were optimized and compared in terms of its composition (based on the total material extracted, total content in carbohydrates, phenolic and flavonoid compounds) and its preservative potential (based on the response of four in vitro antioxidant assays). The key variables of each extraction technique (time, temperature or power and hydroalcoholic mixture) were evaluated by specific experimental designs using response surface methodology. Mathematical models were developed and numerical optimal values for each extraction technique and response were achieved. Regarding the extraction of target compositional compounds, MAE was the most efficient, closely followed by MA. In terms of its preservative potential, MAE was the most suitable solution, but MA gave similar results at lower temperatures (~90 °C). Globally, MA and MAE were the best options conducting to optimal solutions using reduced amounts of ethanol. UAE required higher ethanol contents (~60%). The results showed alternatives to obtain extracts of A. unedo fruits, supporting their potential to be exploited at industrial level.


Assuntos
Ericaceae/química , Manipulação de Alimentos/métodos , Frutas/química , Micro-Ondas , Compostos Fitoquímicos/isolamento & purificação , Ultrassom , Carboidratos/isolamento & purificação , Simulação por Computador , Flavonoides/isolamento & purificação , Modelos Teóricos , Fenóis/isolamento & purificação , Solventes/química , Temperatura Ambiente , Fatores de Tempo
15.
Phytochemistry ; 151: 32-41, 2018 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-29660562

RESUMO

Eleven previously undescribed lanostane-type triterpene glycosides, hebecarposides A-K, were isolated from the leaves of Lyonia ovalifolia var. hebecarpa (Ericaceae), along with two known analogues, lyonifolosides L and O. The structures of hebecarposides A-K were established by extensive spectroscopic analysis and chemical methods, and the absolute configuration of C-24 in hebecarposides A and E was determined to be S and R, respectively, by a Mo2(OAc)4-induced electronic circular dichroism method. This is the first report of the presence of lanostane-type triterpene glycosides in L. ovalifolia var. hebecarpa. All compounds were evaluated for their antiproliferative activities against five cancer cell lines, SMMC-7721, HL-60, SW480, MCF-7, and A-549, and a normal epithelial cell line BEAS-2B, and none of them showed general cytotoxity to the normal cell line BEAS-2B. Interestingly, hebecarposides C, D, G, and K selectively inhibited the proliferation of HL-60 and SMMC-7721 cell lines, and hebecarposides C and D showed significant anti-proliferative activities against A-549 cell lines than the positive control, cis-platin. In addition, hebecarposides C and H exhibited more potent anti-proliferative activities against MCF-7 than cis-platin.


Assuntos
Antineoplásicos Fitogênicos/farmacologia , Ericaceae/química , Glicosídeos/farmacologia , Lanosterol/análogos & derivados , Folhas de Planta/química , Triterpenos/farmacologia , Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/isolamento & purificação , Linhagem Celular , Proliferação de Células/efeitos dos fármacos , Relação Dose-Resposta a Droga , Ensaios de Seleção de Medicamentos Antitumorais , Glicosídeos/química , Glicosídeos/isolamento & purificação , Humanos , Lanosterol/química , Lanosterol/isolamento & purificação , Lanosterol/farmacologia , Modelos Moleculares , Conformação Molecular , Relação Estrutura-Atividade , Triterpenos/química , Triterpenos/isolamento & purificação
16.
Zhongguo Zhong Yao Za Zhi ; 43(5): 964-969, 2018 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-29676095

RESUMO

By the means of chromatographic methods and spectroscopic evidences, 7 diterpenoids were isolated and identified from the roots of Pieris formosa. These known compounds are elucidated as secorhodomollolide C(1), pierisoid B (2), secorhodomollolide B (3), secorhodomollolide A (4), pierisformotoxin G (5), pierisformotoxin B (6) and pierisformotoxin A (7). Compounds 3, 4 were obtained from this plant for the first time. The analgesic activities of compounds 1-7 were evaluated using an acetic acidinduced writhing test in mice. Compounds 3, 4, 6, and 7 exhibited significant analgesic activity at 5 mg·kg;⁻ (ip) compared to vehicle-injected mice (P<0.05). The writhe inhibition rates of compounds 3, 4, 6 and 7 at 5 mg·kg⁻¹ (ip) were 41.3%, 39.4%, 38.6% and 37.5%, respectively.


Assuntos
Analgésicos/farmacologia , Diterpenos/farmacologia , Ericaceae/química , Raízes de Plantas/química , Analgésicos/isolamento & purificação , Animais , Diterpenos/isolamento & purificação , Camundongos , Compostos Fitoquímicos/isolamento & purificação , Compostos Fitoquímicos/farmacologia
17.
Int J Biol Macromol ; 107(Pt B): 1463-1473, 2018 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-28987800

RESUMO

This study investigates the properties of films made from fish myofibrillar protein (FMP) incorporated with combinations of catechin-Kradon extract (C/K) (20%, w/w) at different ratios. The experimental films were compared to a polyvinyl chloride (PVC). The thickness and the transparency of the films were in the range of 0.014-0.015mm and 3.65-3.77, respectively. Significant decreases were observed in elongation at break (35-122%), water vapor permeability (0.54-1.26×10-10gm-1s-1Pa-1) properties, and a gradual decrease in L* value was pronounced when the proportions of catechins were increased (P<0.05). FMP films incorporated with C/K demonstrated to have very good barrier properties to UV light and also enhanced the thermal stability of the developed FMP films. All film samples had a smooth surface; however coarser surfaces were observed when the amounts of catechins were increased. Furthermore, the developed FMP films showed high antioxidant capability. However, no significant antimicrobial activity was observed in this film. It is determined that FMP films enriched with C/K can be used for food packaging, with particular potential for active packaging. However, as compared with the PVC, the FMP films need further development, especially in terms of their mechanical and water barrier properties.


Assuntos
Catequina/química , Ericaceae/química , Proteínas de Peixes/química , Fibras Musculares Esqueléticas/química , Extratos Vegetais/química , Antibacterianos/farmacologia , Antioxidantes/farmacologia , Bactérias/efeitos dos fármacos , Cor , Testes de Sensibilidade Microbiana , Permeabilidade , Solubilidade , Espectroscopia de Infravermelho com Transformada de Fourier , Vapor , Termogravimetria
18.
J Ethnopharmacol ; 215: 83-90, 2018 Apr 06.
Artigo em Inglês | MEDLINE | ID: mdl-29288828

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Strawberry tree (Arbutus unedo L., Ericaceae) leaves represent a potent source of biologically active compounds and have been used for a long to relieve symptoms of various health impairments and diseases. Two major compounds related to their beneficial activities in animals and humans are arbutin and hydroquinone. AIM OF THE STUDY: To establish potential benefit/risk ratio associated with daily oral administration of strawberry tree water leaf extract, arbutin and hydroquinone in doses expected to be non-toxic. MATERIALS AND METHODS: We performed a 14-day and a 28-day study on male and female Lewis rats and evaluated main haematological parameters and the effects of treatments on the levels of primary DNA damage in white blood cells (WBC) using the alkaline comet assay. RESULTS: Our findings suggest no significant changes in the haematological parameters following prolonged exposure to strawberry tree water leaf extract, arbutin, and hydroquinone. However, hydroquinone causes increased, and extract as well as arbutin decreased WBC count in male rats compared to control after 14 days of treatment. DNA damage measured in WBC of rats treated with all compounds was below 10% of the DNA in the comet tail, which indicates low genotoxicity. The genotoxic potential of strawberry water leaf extract was within acceptable limits and reflected effects of a complex chemical composition upon DNA. We also observed slight gender- and exposure time- related differences in primary DNA damage in the leucocytes of control and treated rats. CONCLUSIONS: Future studies should investigate which doses of strawberry tree water leaf extract would be most promising for the potential use as a substitute for bearberry leaves for treatment of urinary infection.


Assuntos
Arbutina/farmacologia , Dano ao DNA/efeitos dos fármacos , Ericaceae/química , Hidroquinonas/farmacologia , Extratos Vegetais/farmacologia , Folhas de Planta/química , Animais , Arbutina/química , Hidroquinonas/química , Leucócitos/efeitos dos fármacos , Extratos Vegetais/química , Ratos , Ratos Endogâmicos Lew
19.
Nat Prod Res ; 32(2): 222-224, 2018 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-28629223

RESUMO

The essential oil isolated from Erica spiculifolia Salisb. by hydrodistillation was analysed by GC-MS. One hundred compounds representing 92.6% of total oil were identified. Among the detected compounds 38 were monoterpenoids (46.2%), 30 were sesquiterpenoids (31.7%), 2 diterpene (0.4%) and 30 compounds (14.3%) were with various non-terpenoid structures. Oxygenated monoerpenes were by far the main class (45.3%) with α-terpineol (7.5%) endo-borneol (7.2%), pinocarveol (5.9%) and thymol (3.7%) as the major compounds. The predominant components of sesquiterpenes were caryophyllene oxide (5.0%), caryophyllene (4.2%), τ -murrolol (3.5%), spathulenol (2.9) and α-cadinol (2.3%).


Assuntos
Ericaceae/química , Óleos Voláteis/análise , Óleos Voláteis/química , Cicloexenos/análise , Cromatografia Gasosa-Espectrometria de Massas , Monoterpenos/análise , Sesquiterpenos/análise , Terpenos/análise
20.
J Agric Food Chem ; 65(46): 9934-9949, 2017 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-29083170

RESUMO

Secondary metabolites, particularly the grayanane diterpenoids produced by the members of genus Pieris, have been investigated in past decades for their remarkable antifeedant and insecticidal activities and toxicity. Grayanoids exhibit diverse biological properties such as antifeedant, insecticidal, cAMP regulatory, and sodium-channel-modulating activities. Structural complexity and diverse bioactivity of grayanoids have made them attractive targets for chemical, biological, and synthetic purposes. The current review synthesized findings published from 1966 to 2017, which include 135 reports that describe 130 terpenoids, including 103 grayanoids. The distribution, structure, skeleton, as well as the antifeedant and insecticidal activity of terpenoids, particularly the grayanane diterpenoids, are discussed in detail in this review. In cases where sufficient information is available, the structure-activity relationships of their antifeedant activity are also presented. We hope that this contribution will prompt more scientists to pay attention to these diterpenoids, which may be potentially applied in the agricultural field.


Assuntos
Ericaceae/química , Inseticidas/química , Inseticidas/farmacologia , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Terpenos/química , Terpenos/farmacologia , Animais , Insetos/efeitos dos fármacos , Insetos/fisiologia , Relação Estrutura-Atividade
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