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1.
Sci Total Environ ; 707: 136024, 2020 Mar 10.
Artigo em Inglês | MEDLINE | ID: mdl-31972909

RESUMO

Biochars have drawn wide attention as adsorbents, carbon sequesters and soil re-mediators. However, these substances are ambiguous regarding their effects on the motility, phenotypic changes and potential adaptative mechanisms of soil organisms. This study investigated how peanut shell biochar (PBC) affects the C. elegans model via a one-choice selection test and RNA-seq analysis. The results showed that C. elegans were able to select either PBC or a water control, and a clear preference for PBC was observed after 48 h of exposure, with the chemotaxis index (CI) reaching approximately 1.0. The nematode preferences for PBC vs sterile PBC/graphite were not significant, which demonstrated that initial microorganisms and appearances were not the reasons for the worms' selection, but the selection behaviour was instead determined by volatile odours. The treatments also showed that biochar amendment significantly decreased the body length, brood size and superoxide dismutase (SOD) activity of C. elegans to 960.20 ± 15.23 µm, 173.22 ± 4.56, 165.81 ± 3.82 U/mL SOD, respectively. Then, a possible molecular mechanism of PBC-induced developmental and reproductive effects on C. elegans was explored. Differential gene expression analysis was performed, and 1625 genes (1425 up- and 225 downregulated genes) were regulated in response to PBC treatment. The top 20 regulated genes were col genes (col-129; col-81; col-139; col-71), bli-6, perm-4 and his-24, which indicated that cuticle collagen synthesis, eggshell formation and/or heterochromatin in postembryonic growth may be disrupted following exposure to PBC. Therefore, our study suggested that quality standards be used to test nematode preferences and responses to biochar amendment, with the aim of safe application in soils, seedling substrates or fertilizers.


Assuntos
Arachis , Caenorhabditis elegans/fisiologia , Carvão Vegetal , Transcriptoma , Animais , Escala de Avaliação Comportamental
2.
BMC Res Notes ; 12(1): 613, 2019 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-31547866

RESUMO

OBJECTIVE: Although studies have shown inconsistent results in terms of prevalence of eating disorders, the Eating Attitudes Test (EAT-26) was used to screen students for abnormal eating behaviors. The results of the self-reported EAT-26 and body frame, as well as the efficacy of using self-administered questionnaires (SAQs) were examined to detect eating disorders in new college students. RESULTS: An anonymous questionnaire (EAT-26) was provided to 7738 new students; 4552 (58.8%) responders were included in the final analysis. Semi-structured interviews were conducted for 131 (1.7%) students. Among them, 6 students showed a high EAT-26 score, but were not diagnosed with an eating disorder based on the Structured Clinical Interview for DSM-IV Axis I Disorders (SCID-I). Three students were diagnosed with an eating disorder using SCID-I, but their EAT-26 scores were below the threshold. From these results, in a non-clinical population, findings on EAT-26 do not agree with those on SCID-I in terms of the diagnosis of eating disorders, and this battery is not appropriate for detecting eating disorders.


Assuntos
Testes Anônimos/métodos , Escala de Avaliação Comportamental/estatística & dados numéricos , Comportamento Alimentar/psicologia , Transtornos da Alimentação e da Ingestão de Alimentos/diagnóstico , Adolescente , Adulto , Índice de Massa Corporal , Transtornos da Alimentação e da Ingestão de Alimentos/epidemiologia , Transtornos da Alimentação e da Ingestão de Alimentos/psicologia , Feminino , Humanos , Japão/epidemiologia , Masculino , Prevalência , Autorrelato , Estudantes , Inquéritos e Questionários
3.
J Genet Psychol ; 180(4-5): 185-204, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31364503

RESUMO

A diverse array of proposed antecedents of social aggression (SA) were examined in two studies. This work is informed by ecological systems theory and examined predictors from the micro- and mesosystems. In Study 1, women (n = 284). completed measures of aggression, digit ratio, emotional intelligence (EI), agreeableness, parenting styles, attachment insecurity with parents and peers, and television (TV) consumption. Aggression correlated with EI, agreeableness, parental authoritarianism, attachment insecurity with fathers and peers, and TV consumption; these variables accounted for 22% of the variance in SA. Father attachment insecurity moderated the TV-SA association, suggesting that positive experiences with parents can buffer negative effects of TV consumption. Study 2 aimed to increase the predictive power of the model. Females (n = 115) completed measures of aggression, digit ratio, EI, agreeableness, parental authoritarianism, interparental conflict, dominance goals, popularity goals, physical appearance comparison, and TV consumption. Aggression correlated with EI, agreeableness, interparental conflict, dominance goals, popularity goals, and appearance comparison. Together, these variables accounted for 40% of the variance in SA; agreeableness, dominance goals, and popularity goals were significant predictors. Interparental conflict moderated the TV-SA association, again suggesting that positive experiences with parents can cushion effects of TV consumption. Implications for development of SA are discussed.


Assuntos
Comportamento do Adolescente/psicologia , Desenvolvimento do Adolescente , Agressão/psicologia , Modelos Psicológicos , Adolescente , Antropometria , Escala de Avaliação Comportamental , Inteligência Emocional , Conflito Familiar/psicologia , Feminino , Dedos/anatomia & histologia , Humanos , Relações Pais-Filho , Grupo Associado , Aparência Física , Televisão , Adulto Jovem
4.
Arch. Clin. Psychiatry (Impr.) ; 46(4): 89-96, July-Aug. 2019. tab, graf
Artigo em Inglês | LILACS | ID: biblio-1019345

RESUMO

Abstract Background Based on his model of self-regulation and executive functions, Barkley developed a self- and other-report questionnaire (the Barkley Deficits in Executive Functioning Scale - BDEFS). The BDEFS measures deficits in executive functions as expressed in daily life activities like self-management of time, self-organization, self-restraint, self-motivation, and self-regulation of emotion. Objectives This study created and analyzed a Dutch translation and adaptation in conformance with official guidelines. Methods The Dutch and English BDEFS were completed by 25 bilingual Dutch adults to evaluate semantic correspondence. Consequently, 60 Dutch participants completed the Dutch BDEFS, Barratt Impulsiveness Scale-Eleventh edition (BIS-11) and the Dysexecutive Questionnaire (DEX) to evaluate concurrent validity and internal consistency. Results The versions demonstrated sufficient semantic equivalence and Spearman's rho of total scores was high; items mostly showed moderate-to-high correlations. Regression analysis showed no proportional bias. Internal consistency was also high. Correlations between BDEFS, BIS-11 and DEX supported concurrent validity. Discussion We conclude that a successful BDEFS translation and adaptation was created with satisfactory reliability and validity. Further research should assess the utility of the BDEFS in Dutch clinical practice.


Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Tradução , Inquéritos e Questionários , Função Executiva/fisiologia , Escala de Avaliação Comportamental , Psicometria , Valor Preditivo dos Testes , Reprodutibilidade dos Testes , Autogestão/psicologia , Países Baixos
5.
Sch Psychol ; 34(5): 531-540, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31169380

RESUMO

The purpose of this study was to evaluate the reliability, validity, and accuracy of scores from the Intervention Selection Profile-Function (ISP-Function): a brief functional assessment tool founded upon Direct Behavior Rating (DBR) methodology. Participants included 34 teacher-student dyads. Using the ISP-Function, teachers rated the extent to which students exhibited disruptive behavior, as well as the frequency with which disruptions were met with four consequences. Ratings were completed across three 10-min sessions, during which a research assistant also collected systematic direct observation (SDO) data regarding the same behavior and consequences. Results indicated adequate temporal reliability (≥.70) was attained for the adult attention and peer attention targets across the three ratings; in contrast, up to 8-18 data points would be needed to achieve adequate reliability across the remaining targets. Findings further suggested that while ISP-Function ratings of disruptive behavior, adult attention, and peer attention were moderately to highly correlated with SDO data, correlations were in the low range for the access to items/activities and escape/avoidance targets. Finally, analysis of difference scores showed that on average, mean ISP-Function scores fell within only 0.33 to 1.81 points of mean SDO scores (on the 0-10 DBR scale). Agreement coefficients indicative of exact score agreement were less consistent, suggesting accuracy ranged from poor to substantial. Results are promising, but future research is necessary to support applied ISP-Function use. (PsycINFO Database Record (c) 2019 APA, all rights reserved).


Assuntos
Escala de Avaliação Comportamental/normas , Comportamento Infantil , Comportamento Problema , Estudantes , Técnicas de Observação do Comportamento , Criança , Feminino , Humanos , Masculino , Reprodutibilidade dos Testes , Professores Escolares
6.
Behav Processes ; 165: 51-57, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31121215

RESUMO

Behavioral economic measures have demonstrated marked success in the evaluation of consumer choice. Field-standard operant demand curve analyses provide a valuable model of resource allocation via responses to maintain "free-rate" commodity use or consumption. This demand analysis thereby provides a behavioral complement to consumer science techniques. Despite apparent congruence of operant behavioral economics and consumer science, the left-digit effect represents one area of research predominantly untouched by behavioral economic investigation. Previous efforts have applied the hypothetical purchase task to map the effect of a changing left-digit on subsequent purchase decisions. The current study extends investigation of the phenomenon to responding on the validated Alcohol Purchase Task. Introduction of a high-density price structure revealed evidence of digit sensitivity, wherein demand elasticity was disproportionately affected at and around whole-dollar changes. That responses were influenced by small shifts in pricing implies a possibility for policy-level modulation of alcohol ingestion without need to increase commodity price beyond unit elasticity. Capture of digit preference in a self-report framework speaks to the sensitivity of purchase task methodology to detect small, aberrant changes in consumer product perception. Behavioral economic researchers should consider this extent of sensitivity when interpreting results of hypothetical purchase task investigations.


Assuntos
Consumo de Bebidas Alcoólicas/economia , Consumo de Bebidas Alcoólicas/psicologia , Comportamento de Escolha , Condicionamento Operante , Custos e Análise de Custo , Economia Comportamental , Alocação de Recursos , Adulto , Escala de Avaliação Comportamental , Tomada de Decisões , Feminino , Humanos , Masculino , Motivação , Aquisição Baseada em Valor
7.
Clin Neuropharmacol ; 42(4): 123-130, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31045589

RESUMO

OBJECTIVES: The aim of this open-label study was to investigate the long-term safety and efficacy of selegiline as monotherapy in Japanese patients with early Parkinson disease (PD). METHODS: We conducted a 56-week prospective study in patients with early PD (N = 134) who had previously completed the randomized, double-blind, placebo-controlled phase III trial of selegiline monotherapy for 12 weeks. In the present study, dosing was titrated from 2.5 to 10 mg/d in increments of 2.5 mg/d for 2 weeks. From the seventh week, the dosage was maintained at 10 mg/d until week 56. The primary outcome was any change in the total Unified Parkinson's Disease Rating Scale (UPDRS) score (part I + II + III) from baseline. Secondary outcomes, including changes in the UPDRS subscores and safety profile, were also evaluated. RESULTS: Ninety-one (67.9%) patients completed the 56-week study. Treatment with selegiline significantly reduced total UPDRS score from week 4 (mean ± SD, -2.62 ± 3.83; P < 0.0001) to week 56 (-3.39 ± 9.27; P < 0.01). The peak effect was seen at week 20 (-5.79 ± 5.57; P < 0.0001). In addition, we found similar improvements in the UPDRS parts II and III scores. The incidence rate of adverse drug reactions was 44.3% (58 patients) and did not increase during the period of 10 mg selegiline administration. CONCLUSIONS: Long-term monotherapy with selegiline (10 mg/d) was effective and well tolerated in patients with early PD in this 56-week study.


Assuntos
Antiparkinsonianos/uso terapêutico , Inibidores da Monoaminoxidase/uso terapêutico , Doença de Parkinson/tratamento farmacológico , Selegilina/uso terapêutico , Adulto , Idoso , Idoso de 80 Anos ou mais , Antiparkinsonianos/administração & dosagem , Escala de Avaliação Comportamental , Método Duplo-Cego , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Inibidores da Monoaminoxidase/administração & dosagem , Estudos Prospectivos , Selegilina/administração & dosagem
8.
Clin Child Psychol Psychiatry ; 24(4): 791-808, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31074289

RESUMO

The Conners' Rating Scales are widely used to assess attention deficit/hyperactivity disorder (ADHD) and related difficulties in children and adolescents. A short form of the scales is available, which, along with the several advantages of brief versions, also displays good psychometric properties. Nonetheless, no studies have confirmed them in cultural contexts different from the original one. The present study examined the psychometric properties of the Self-Report, Parent, and Teacher Conners 3-Short Forms in terms of reliability and validity in an Italian sample. Analyses were performed on 591 children and adolescents, 631 parents' ratings, and 325 teachers' ratings. To test for discriminative validity, ADHD clinical samples of 55 youth, 63 parents, and 15 teachers were compared to gender- and age-matched groups. Findings confirmed the original multidimensional structures and supported the Conners 3-Short Form scales as reliable and valid tools to assess ADHD and its main comorbid conditions.


Assuntos
Transtorno do Deficit de Atenção com Hiperatividade/diagnóstico , Escala de Avaliação Comportamental/normas , Escalas de Graduação Psiquiátrica/normas , Psicometria/normas , Adolescente , Criança , Feminino , Humanos , Itália , Masculino , Pais , Reprodutibilidade dos Testes , Professores Escolares
9.
Behav Res Methods ; 51(4): 1498-1509, 2019 08.
Artigo em Inglês | MEDLINE | ID: mdl-31065937

RESUMO

Humans often say they prefer certain attributes and trait levels and yet choose options inconsistent with those preferences, a phenomenon known as the stated-revealed preference gap. In this article, we compare preferences and choices in the decision to adopt a dog, a social-choice problem that is largely one-sided. We used existing and newly gathered field data about the dog adoption process to study how people make their choices of companion animals and how those choices can be improved. We found that in the real-world choice of dogs within an animal shelter, individuals generally showed a large amount of overlap between their stated preferences and their ratings of the traits of their chosen dog. However, there was little relationship between an adopter's perceptions of their chosen dog's behavioral traits and third-party in-shelter behavior evaluations of the same dogs, suggesting that it is difficult to predict which dogs will satisfy an adopter's preferences. We also tested which commonly collected factors impact how quickly dogs are adopted from animal shelters. Overall, this work provides insight into the process of combining experimentally collected data and big data to elucidate choice behavior.


Assuntos
Comportamento de Escolha , Animais de Estimação , Animais , Escala de Avaliação Comportamental , Coleta de Dados , Tomada de Decisões , Cães , Feminino , Humanos , Masculino
10.
An. psicol ; 35(2): 290-299, mayo 2019. tab, graf
Artigo em Inglês | IBECS | ID: ibc-181699

RESUMO

Creativity and actions aimed at fostering it became a matter of interest in higher education. Since creativity is a multidimensional phenomenon, its diverse dimensions must be assessed. Among them, personality arises as the most relevant one. Therefore, the local adaptation of Gough’s Creative Personality Scale to be used in college students is proposed. This scale is composed of adjectives to be responded according to the examinees’ identification with each one of them. Two independent samples (n= 150 each) were employed to develop an exploratory factor analysis and an internal consistency analysis, on the one hand, and a confirmatory factor analysis and a convergent validity study. The exploratory procedure retained eight items and a 1-dimension structure with a good internal consistency (ordinal alpha= .853). This model was empirically verified by the confirmatory analysis which showed good fit indices, as well as an adequate internal consistency coefficient. Moreover, adequate convergent validity evidences were obtained. Findings are discussed taking into account the theoretical model and the study weaknesses along with practical and theoretical outcomes. Further research lines are proposed


La creatividad y las prácticas ligadas a fomentarla en el ámbito universitario constituyen en la actualidad un tema de interés. La creatividad en tanto fenómeno multidimensional requiere ser evaluada en sus distintas facetas, siendo la personalidad una de las que mayor relevancia reviste. Se propone así, adaptar localmente la Escala de Personalidad Creativa (EPC) de Gough para ser aplicada en el ámbito universitario. El instrumento se compone de 30 adjetivos a ser respondidos por los evaluados según se identifiquen o no con cada uno de ellos. Se realizó un análisis factorial exploratorio y de consistencia interna (n = 150) y otro confirmatorio y de validez convergente (n = 150) a partir de dos muestras independientes. El procedimiento exploratorio conservó ocho ítems y una estructura factorial unidimensional con una óptima consistencia interna (alfa ordinal = .853). Este modelo se verificó empíricamente mediante el análisis confirmatorio que arrojó óptimos índices de ajuste y un apropiado índice de consistencia interna. Adicionalmente se obtuvieron adecuadas evidencias de validez convergente del instrumento en relación a otros constructos teóricamente afines. Los resultados se discuten a la luz de la teoría y las limitaciones del diseño, destacando los alcances del estudio y las futuras líneas de investigación


Assuntos
Humanos , Masculino , Feminino , Adolescente , Adulto Jovem , Adulto , Pessoa de Meia-Idade , Testes de Personalidade , Criatividade , Escala de Avaliação Comportamental , Psicometria/instrumentação , Argentina/epidemiologia , Determinação da Personalidade/estatística & dados numéricos , Transtornos da Personalidade/diagnóstico , Estudantes/psicologia , Reprodutibilidade dos Testes
11.
Eur J Pediatr ; 178(7): 995-1003, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31030258

RESUMO

Primary ciliary dyskinesia (PCD) causes a broad spectrum of disease. This study aims to explore the developmental, behavioral, and social-emotional aspects of preschool-aged children with PCD. Fourteen PCD, 17 cystic fibrosis (CF) patients and 15 healthy subjects were enrolled. Developmental features of the participants were evaluated with Ages and Stages Questionnaire. Parents of participants filled out the Child Behavior Checklist (CBCL). The number of children screened positive for developmental delay was statistically higher in the PCD group. Higher numbers of children with PCD were screened positive for developmental delay in communication and problem-solving domains. Delay in fine motor skill domain was more common in children with PCD and CF compared to healthy subjects. There was no difference among the three groups in terms of gross motor and personal-social development. None of the children in all three groups was shown to have social-emotional problems. In CBCL, patients with CF had higher internalizing problem scores. Externalizing and total problem scores did not differ between the three groups. However, among PCD patients, children with developmental delay on more than one domain had higher externalizing and total problem scores.Conclusion: The current study revealed that positive screening for developmental delay is more common in preschool-aged PCD patients compared to patients with CF and healthy children. What is Known: • Intelligence scores of school-aged PCD patients are similar to healthy subjects despite their higher internalizing problem scores on Child Behavior Checklist (CBCL). • School-aged PCD patients exhibit higher hyperactivity and inattention findings. What is New: • Positive screening for developmental delay in communication, problem-solving and fine motor skills is more common in preschool-aged PCD patients. • Preschool-aged PCD patients screened positive for developmental delay in more than one domain have higher externalizing and total problem scores on CBCL.


Assuntos
Transtornos do Comportamento Infantil/etiologia , Transtornos da Motilidade Ciliar/complicações , Deficiências do Desenvolvimento/etiologia , Escala de Avaliação Comportamental , Estudos de Casos e Controles , Transtornos do Comportamento Infantil/diagnóstico , Pré-Escolar , Transtornos da Motilidade Ciliar/fisiopatologia , Transtornos da Motilidade Ciliar/psicologia , Estudos de Coortes , Deficiências do Desenvolvimento/diagnóstico , Feminino , Humanos , Masculino , Fatores de Risco
12.
Cad Saude Publica ; 35(3): e00179318, 2019 04 08.
Artigo em Português | MEDLINE | ID: mdl-30970100

RESUMO

The Tripartite Influence Scale (TIS) is used to assess the influence of social and family factors on individuals' body image. However, little evidence has been presented on the validity of the TIS construct. The study aimed to estimate the psychometric indicators in TIS and identify the degree of influence of the media, parents, and peers on body image in university students, considering different characteristics. University students of both sexes participated. The psychometric indicators in TIS were assessed for each sex using confirmatory factor analysis. Mean scores were calculated for each TIS factor. Prevalence rates for degree of influence were presented. A multivariate regression model was built to verify the relationship between target characteristics and TIS factor scores. The sample included 791 university students (63.2% women) 18 to 40 years of age. TIS did not initially display a good fit to the samples and was therefore refined. After refinement, the scale showed adequate validity and reliability for women and men. Significant differences were observed in the mean scores between women and men, and in the prevalence rates the majority of the students were in the low category. Work, consumption of food supplements for body changes, age, body mass index, self-rated diet, and level of physical activity were significantly related to TIS factors. Women were generally more influenced than men by the media and peers. Characteristics identified as related to TIS can be relevant for inclusion in clinical and research protocols.


Assuntos
Escala de Avaliação Comportamental/estatística & dados numéricos , Imagem Corporal/psicologia , Família/psicologia , Estudantes/psicologia , Inquéritos e Questionários/estatística & dados numéricos , Adolescente , Adulto , Índice de Massa Corporal , Brasil , Estudos Transversais , Análise Fatorial , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Psicometria , Reprodutibilidade dos Testes , Fatores Sexuais , Fatores Socioeconômicos , Universidades , Adulto Jovem
13.
J Vis Exp ; (145)2019 03 02.
Artigo em Inglês | MEDLINE | ID: mdl-30882789

RESUMO

Pharmacological and toxicological studies in neurodegeneration require comprehensive behavioral analysis in mice because motor dysfunctions and dysfunctions in mood and cognition are common and often shared symptoms in neurodegenerative diseases. Shown here is a behavioral test battery for motor, mood, and cognition, which can be repeatedly tested in a longitudinal study. This battery assesses the overall behavioral phenotype in mice by examining each domain of behavior with at least two independent well-accepted tests (i.e., open-field test and rotarod test for motor function, social interaction test, elevated plus maze test, and forced swim test for emotional function, and Morris water maze test and novel object recognition test for cognitive function). Therefore, this sensitive and comprehensive test battery is a powerful tool for the study of behavioral alternation in neurodegeneration.


Assuntos
Comportamento Animal/fisiologia , Cognição/fisiologia , Emoções/fisiologia , Destreza Motora/fisiologia , Animais , Escala de Avaliação Comportamental , Comportamento Animal/efeitos dos fármacos , Cognição/efeitos dos fármacos , Emoções/efeitos dos fármacos , Masculino , Aprendizagem em Labirinto , Camundongos Endogâmicos C57BL , Destreza Motora/efeitos dos fármacos , Nanopartículas/toxicidade , Teste de Desempenho do Rota-Rod , Dióxido de Silício/toxicidade , Natação
14.
Intellect Dev Disabil ; 57(2): 79-94, 2019 04.
Artigo em Inglês | MEDLINE | ID: mdl-30920913

RESUMO

Down syndrome (DS) is characterized by difficulties in both intellectual functioning and adaptive behavior. These sets of abilities are considered as separate but related domains with small to moderate correlations. The main objective of this study was to explore the relationship of intellectual functioning and adaptive behavior in adolescents with DS because previous studies have shown different relationship patterns between these constructs across other syndromes. Fifty-three adolescents with DS were assessed regarding their intellectual functioning whereas adaptive behavior was reported by parents and teachers. Participants showed a better performance on verbal than nonverbal tasks when assessing intellectual functioning, contrary to previous findings. Regarding adaptive behavior, higher social skills were reported than conceptual and practical skills. Intellectual functioning and adaptive behavior showed a medium correlation, consistent with observations in typical population. These results support the exploration of the variability across the DS phenotype.


Assuntos
Adaptação Psicológica , Síndrome de Down/psicologia , Inteligência , Adolescente , Escala de Avaliação Comportamental , Criança , Feminino , Humanos , Deficiência Intelectual/genética , Deficiência Intelectual/psicologia , Testes de Inteligência , Masculino , Classe Social , Habilidades Sociais
15.
Biochim Biophys Acta Mol Basis Dis ; 1865(6): 1477-1489, 2019 06 01.
Artigo em Inglês | MEDLINE | ID: mdl-30826466

RESUMO

BACKGROUND: Maternal immune activation (MIA) is an independent risk factor for psychiatric disorders including depression spectrum in the offsprings, but the molecular mechanism is unclear. Recent studies show that interferon-stimulated gene-15 (ISG15) is involved in inflammation and neuronal dendrite development; here we studied the role of ISG15 in MIA-induced depression and the underlying mechanisms. METHODS: By vena caudalis injecting polyinosinic: polycytidylic acid (poly I:C) into the pregnant rats to mimic MIA, we used AAV or lentivirus to introduce or silence ISG15 expression. Synaptic plasticity was detected by confocal microscope and Golgi staining. Cognitive performances of the offspring were measured by Open field, Forced swimming and Sucrose preference test. RESULTS: We found that MIA induced depression-like behaviors with dendrite impairments in the offspring with ISG15 level increased in the offsprings' brain. Overexpressing ISG15 in the prefrontal cortex of neonatal cubs (P0) could mimic dendritic pathology and depressive like behaviors, while downregulating ISG15 rescued these abnormalities in the offsprings. Further studies demonstrated that MIA-induced upregulation of inflammatory cytokines promoted ISG15 expression in the offspring' brain which suppressed Rap2A ubiquitination via NEDD4 and thus induced Rap2A accumulation, while upregulating NEDD4 abolished ISG15-induced dendrite impairments. CONCLUSIONS: These data reveal that MIA impedes offsprings' dendrite development and causes depressive like behaviors by upregulating ISG15 and suppressing NEDD4/Rap2A signaling. The current findings suggest that inhibiting ISG15 may be a promising intervention of MIA-induced psychiatric disorders in the offsprings.


Assuntos
Citocinas/genética , Dendritos/metabolismo , Proteínas de Ligação ao GTP/genética , Ubiquitina-Proteína Ligases Nedd4/genética , Efeitos Tardios da Exposição Pré-Natal/genética , Ubiquitinas/genética , Animais , Escala de Avaliação Comportamental , Citocinas/antagonistas & inibidores , Citocinas/imunologia , Dendritos/imunologia , Dendritos/patologia , Depressão , Modelos Animais de Doenças , Feminino , Proteínas de Ligação ao GTP/antagonistas & inibidores , Proteínas de Ligação ao GTP/imunologia , Regulação da Expressão Gênica , Imunidade Inata/efeitos dos fármacos , Inflamação , Injeções Intravenosas , Ubiquitina-Proteína Ligases Nedd4/antagonistas & inibidores , Ubiquitina-Proteína Ligases Nedd4/imunologia , Neurogênese/efeitos dos fármacos , Neurogênese/genética , Neurogênese/imunologia , Plasticidade Neuronal/efeitos dos fármacos , Plasticidade Neuronal/genética , Plasticidade Neuronal/imunologia , Poli I-C/administração & dosagem , Córtex Pré-Frontal/imunologia , Córtex Pré-Frontal/metabolismo , Córtex Pré-Frontal/patologia , Gravidez , Efeitos Tardios da Exposição Pré-Natal/induzido quimicamente , Efeitos Tardios da Exposição Pré-Natal/imunologia , Efeitos Tardios da Exposição Pré-Natal/patologia , RNA Interferente Pequeno/genética , RNA Interferente Pequeno/metabolismo , Ratos , Ratos Sprague-Dawley , Transdução de Sinais , Ubiquitinas/antagonistas & inibidores , Ubiquitinas/imunologia
16.
Sch Psychol ; 34(3): 261-270, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30883158

RESUMO

The purposes of this study were twofold. The first was to use latent class analysis to identify groupings of students defined by the presence or absence of academic or behavioral risk. The second was to determine whether these groups differed across various dichotomous academic and behavioral outcomes (e.g., suspensions, office discipline referrals, statewide achievement test failure). Students (N = 1,488) were sampled from Grades 3-5. All students were screened for academic risk using AIMSweb Reading Curriculum-Based Measure and AIMSweb Mathematics Computation, and behavioral risk using the Social, Academic, and Emotional Behavior Risk Screener (SAEBRS). Latent class analyses supported the fit of a three-class model, with resulting student classes defined as low-risk academic and behavior (Class 1), at-risk academic and high-risk behavior (Class 2), and at-risk math and behavior (Class 3). Logistic regression analyses indicated the classes demonstrated statistically significant differences statewide achievement scores, as well as suspensions. Further analysis indicated that the odds of all considered negative outcomes were higher for both groups characterized by risk (i.e., Classes 2 and 3). Negative outcomes were particularly likely for Class 2, with the odds of negative behavioral and academic outcomes being 6-15 and 112-169 times more likely, respectively. Results were taken to support an integrated approach to universal screening in schools, defined by the evaluation of both academic and behavioral risk. (PsycINFO Database Record (c) 2019 APA, all rights reserved).


Assuntos
Desempenho Acadêmico , Escala de Avaliação Comportamental , Comportamento Infantil , Comportamento Problema , Criança , Feminino , Humanos , Masculino , Programas de Rastreamento , Meio-Oeste dos Estados Unidos , Risco , Instituições Acadêmicas
18.
Codas ; 31(1): e20170237, 2019 Feb 25.
Artigo em Português, Inglês | MEDLINE | ID: mdl-30810630

RESUMO

PURPOSE: To characterize and compare behavioral tests of central auditory processing of schoolers of initial grades in two stages, test and retest; and correlate the variables age and gender with the results of these tests. METHODS: Cohort, analytical, observational, longitudinal and prospective study; developed in a public school. The sample included 36 schoolers, divided into two groups considering the schooling: G1- Thirteen children of first grade and G2- Twenty-three children of second grade. The inclusion criteria were audiological assessment within normality patterns and being enrolled in the first or second year of elementary school and, as exclusion criteria, presence of neurological, cognitive and behavioral disorders. The audiological assessment and application of the behavioral tests of central auditory processing occurred in two different moments, with an interval of six months, called test and retest. RESULTS: The test with the highest prevalence of change, in both steps and groups, was Dichotic Digits. It is noteworthy that no schoolers from G1 and some from G2 understood RGDT at the test stage and that even after six months this difficulty remained in both groups. In the retest stage, a significant improvement was noticed in the schoolers' performance of both groups. It was still noticed a correlation between the age variable and dichotic digits test in the left ear in both stages. CONCLUSION: There was a high incidence of alteration in the tests and, a performance improvement was noticed in the retest stage, mainly in the tests of sound localization, dichotic digits and RGDT.


Assuntos
Percepção Auditiva/fisiologia , Testes com Listas de Dissílabos , Estimulação Acústica , Escala de Avaliação Comportamental , Criança , Feminino , Humanos , Alfabetização , Estudos Longitudinais , Masculino , Modelos Teóricos , Estudos Prospectivos , Reprodutibilidade dos Testes
19.
Life Sci Alliance ; 2(1)2019 02.
Artigo em Inglês | MEDLINE | ID: mdl-30692166

RESUMO

EPS15 and its homologous EPS15L1 are endocytic accessory proteins. Studies in mammalian cell lines suggested that EPS15 and EPS15L1 regulate endocytosis in a redundant manner. However, at the organismal level, it is not known to which extent the functions of the two proteins overlap. Here, by exploiting various constitutive and conditional null mice, we report redundant and nonredundant functions of the two proteins. EPS15L1 displays a unique nonredundant role in the nervous system, whereas both proteins are fundamental during embryo development as shown by the embryonic lethality of -Eps15/Eps15L1-double KO mice. At the cellular level, the major process redundantly regulated by EPS15 and EPS15L1 is the endocytosis of the transferrin receptor, a pathway that sustains the development of red blood cells and controls iron homeostasis. Consequently, hematopoietic-specific conditional Eps15/Eps15L1-double KO mice display traits of microcytic hypochromic anemia, due to a cell-autonomous defect in iron internalization.


Assuntos
Proteínas Adaptadoras de Transdução de Sinal/genética , Proteínas Adaptadoras de Transdução de Sinal/metabolismo , Endocitose/fisiologia , Anemia Hipocrômica/genética , Animais , Escala de Avaliação Comportamental , Desenvolvimento Embrionário/fisiologia , Eritrócitos/metabolismo , Fibroblastos/metabolismo , Técnicas de Inativação de Genes , Genes Letais/fisiologia , Hipocampo/citologia , Ferro/metabolismo , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Neurônios/metabolismo , Receptores da Transferrina/metabolismo , Homologia de Sequência de Aminoácidos , Homologia Estrutural de Proteína , Sinapses/metabolismo
20.
Eur J Pharmacol ; 846: 100-108, 2019 Mar 05.
Artigo em Inglês | MEDLINE | ID: mdl-30658114

RESUMO

Autism spectrum disorder (ASD) is a prevalent neurodevelopmental disorder characterized by deficits in social interaction, communication, and repetitive behaviors. BTBR T+ Itpr3tf/J (BTBR) mice, a preclinical autistic model featuring ASD symptoms as defined by social relations, was used in this study. We evaluated the potentially protective effect of D-Ala-peptide T-amide (DAPTA), a selective C-C chemokine receptor 5 (CCR5) antagonist, in BTBR mice. CCR5 is considered a potential therapeutic target in different neurodegenerative disorders. BTBR and C57 mice were intraperitoneally (i.p) treated with the DAPTA (0.01 mg/kg, i.p, once daily) for 7 days. We examined the effect of DAPTA by evaluating marble burying and administering repetitive behavior tests. We employed flow cytometry to assess the effect of DAPTA on CCR5+, CD4+CCR5+, CCR5+IL-6+, CCR5+IL-9+, CCR5+IL-17A+, CCR5+RORγT+, CCR5+IL-10+, and CCR5+Foxp3+ in spleen cells. We further explored the effects of DAPTA on IL-6, IL-9, IL-17A, RORγT, IL-10, and Foxp3 protein and mRNA expression levels in the brain tissues. DAPTA administration significantly decreased marble burying and repetitive behavior in BTBR mice. Additionally, DAPTA treatment inhibited CCR5+, CD4+CCR5+, CCR5+IL-6+, CCR5+IL-9+, CCR5+IL-17A+, CCR5+RORγT+, and upregulated CCR5+IL-10+, and CCR5+Foxp3+ production. We further observed that DAPTA downregulated IL-6, IL-9, IL-17A, and RORγT, and increased IL-10 and Foxp3 protein and mRNA expression. Therefore, our results suggest that DAPTA administration represents a potential treatment strategy for patients with ASD.


Assuntos
Alanina/farmacologia , Transtorno do Espectro Autista/tratamento farmacológico , Antagonistas dos Receptores CCR5/farmacologia , Citocinas/metabolismo , Fatores de Transcrição Forkhead/metabolismo , Interleucinas/metabolismo , Membro 3 do Grupo F da Subfamília 1 de Receptores Nucleares/metabolismo , RNA Mensageiro/metabolismo , Receptores CCR5/metabolismo , Células Th17/metabolismo , Animais , Escala de Avaliação Comportamental , Encéfalo/metabolismo , Modelos Animais de Doenças , Regulação para Baixo , Citometria de Fluxo/métodos , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos , Baço/metabolismo
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