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1.
PLoS One ; 15(8): e0237195, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32764819

RESUMO

This study investigated the caries-preventive effect of 445 nm laser radiation in combination with fluoride on the prevention of white spot lesions. Previously, several studies have indicated the ability of 488 nm argon ion laser irradiation to reduce early enamel demineralization. A diode laser (445 nm) could be an alternative technology for possible caries-preventive potential. Each sample of a group of seventeen caries-free bovine teeth was treated in four different ways on four different zones of the labial surface: control/no treatment (C), laser irradiation only (L) (0.3 W, 60 s and applied dose of 90 J/cm2), amine fluoride application only (10,000 ppm and pH 3.9) (F), and amine fluoride application followed by laser irradiation (FL). After treatment, the teeth were subjected to a demineralization solution (pH 4.3 for 48 h at 37 °C) to induce subsurface lesions. After sectioning, the teeth were examined by light microscopy. Three teeth were analyzed by scanning electron microscopy (SEM). The depths of the subsurface lesions in the C, L, F, and FL groups were 103.01 (± 13.04), 96.99 (± 14.51), 42.59 (± 17.13), and 24.35 (± 11.38) µm, respectively. The pairwise group comparison showed the following results: p < 0.001 for FL versus C, FL versus L, F versus C, and F versus L, p = 0.019 for FL versus F and p = 0.930 for L versus C. The SEM micrographs support the light-microscopic examination. The results of the current study have shown that using relatively low irradiation settings of 445 nm laser on fluoridated enamel may be effective for prevention of white spot lesions.


Assuntos
Cárie Dentária/prevenção & controle , Fluoretos Tópicos/administração & dosagem , Terapia com Luz de Baixa Intensidade/métodos , Desmineralização do Dente/prevenção & controle , Animais , Bovinos , Terapia Combinada/métodos , Esmalte Dentário/efeitos dos fármacos , Esmalte Dentário/efeitos da radiação , Esmalte Dentário/ultraestrutura , Humanos , Lasers Semicondutores/uso terapêutico , Microscopia Eletrônica de Varredura
2.
Acta Odontol Latinoam ; 33(1): 6-13, 2020 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-32621593

RESUMO

Dental enamel defects (DED) are lesions that occur due several factors. Proper care is needed to promote their treatment and prevention. The aim of this study was to evaluate the occurrence of DED in permanent teeth of children who used antimicrobial drugs in the first four years of life. This is a crosssectional study carried out in a Primary Health Care (PHC) service, which included children from six to 12 years of age. DED were evaluated by oral examination, and data on the use of antimicrobials in early childhood were collected based on medical records. Data were analyzed with the chi-square test and Fisher's exact test. The sample included 144 children. In relation to DED, 50% (72) and 20.1% (29) presented opacity and hypoplasia, respectively. Amoxicillin was the most frequently prescribed drug, followed by sulfamethoxazole + trimethoprim. Among the children, 78.5% (113) were prescribed antimicrobial drugs at least once during the first 4 years of life, and 55% (79) of them presented some type of DED. There was no statistically significant association between the variables analyzed. In conclusion, there was high prevalence of children with DED, and amoxicillin was the most commonly prescribed antibiotic.


Assuntos
Antibacterianos/uso terapêutico , Cárie Dentária , Hipoplasia do Esmalte Dentário/induzido quimicamente , Esmalte Dentário/anormalidades , Esmalte Dentário/efeitos dos fármacos , Dente Decíduo/anormalidades , Antibacterianos/efeitos adversos , Criança , Hipoplasia do Esmalte Dentário/epidemiologia , Feminino , Humanos , Masculino , Prevalência , Atenção Primária à Saúde
3.
J Appl Oral Sci ; 28: e20190720, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32428059

RESUMO

Objective This study evaluated the clinical effect of violet LED light on in-office bleaching used alone or combined with 37% carbamide peroxide (CP) or 35% hydrogen peroxide (HP). Methodology A total of 100 patients were divided into five groups (n=20): LED, LED/CP, CP, LED/HP and HP. Colorimetric evaluation was performed using a spectrophotometer (ΔE, ΔL, Δa, Δb) and a visual shade guide (ΔSGU). Calcium (Ca)/phosphorous (P) ratio was quantified in the enamel microbiopsies. Measurements were performed at baseline (T 0 ), after bleaching (T B ) and in the 14-day follow-up (T 14 ). At each bleaching session, a visual scale determined the absolute risk (AR) and intensity of tooth sensitivity (TS). Data were evaluated by one-way (ΔE, Δa, ΔL, Δb), two-way repeated measures ANOVA (Ca/P ratio), and Tukey post-hoc tests. ΔSGU and TS were evaluated by Kruskal-Wallis and Mann-Whitney, and AR by Chi-Squared tests (a=5%). Results LED produced the lowest ΔE (p<0.05), but LED/HP promoted greater ΔE, ΔSGU and Δb (T 14 ) than HP (p<0.05). No differences were observed in ΔE and ΔSGU for LED/CP and HP groups (p>0.05). ΔL and Δa were not influenced by LED activation. After bleaching, LED/CP exhibited greater Δb than CP (p>0.05), but no differences were found between these groups at T 14 (p>0.05). LED treatment promoted the lowest risk of TS (16%), while HP promoted the highest (94.4%) (p<0.05). No statistical differences of risk of TS were found for CP (44%), LED/CP (61%) and LED/HP (88%) groups (p>0.05). No differences were found in enamel Ca/P ratio among treatments, regardless of evaluation times. Conclusions Violet LED alone produced the lowest bleaching effect, but enhanced HP bleaching results. Patients treated with LED/CP reached the same efficacy of HP, with reduced risk and intensity of tooth sensitivity and none of the bleaching protocols adversely affected enamel mineral content.


Assuntos
Peróxido de Carbamida/administração & dosagem , Peróxido de Hidrogênio/administração & dosagem , Luz , Fototerapia/métodos , Clareadores Dentários/administração & dosagem , Clareamento Dental/métodos , Adolescente , Adulto , Análise de Variância , Colorimetria , Terapia Combinada , Esmalte Dentário/efeitos dos fármacos , Esmalte Dentário/efeitos da radiação , Sensibilidade da Dentina/induzido quimicamente , Feminino , Humanos , Masculino , Valores de Referência , Fatores de Risco , Espectrofotometria , Estatísticas não Paramétricas , Propriedades de Superfície/efeitos dos fármacos , Propriedades de Superfície/efeitos da radiação , Resultado do Tratamento , Adulto Jovem
4.
Int J Nanomedicine ; 15: 3181-3191, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32440117

RESUMO

Objective: The aim of this concise review is to summarize the use of silver nanomaterials for caries prevention. Methods: Two researchers independently performed a literature search of publications in English using Embase, Medline, PubMed, and Scopus databases. The keywords used were (silver nanoparticles OR AgNPs OR nano silver OR nano-silver) AND (caries OR tooth decay OR remineralisation OR remineralization). They screened the title and abstract to identify potentially eligible publications. They then retrieved the full texts of the identified publications to select original research reporting silver nanomaterials for caries prevention. Results: The search identified 376 publications, and 66 articles were included in this study. The silver nanomaterials studied were categorized as resin with silver nanoparticles (n=31), silver nanoparticles (n=21), glass ionomer cement with silver nanoparticles (n=7), and nano silver fluoride (n=7). Most (59/66, 89%) studies investigated the antibacterial properties, and they all found that silver nanomaterials inhibited the adhesion and growth of cariogenic bacteria, mainly Streptococcus mutans. Although silver nanomaterials were used as anti-caries agents, only 11 (11/66, 17%) studies reported the effects of nanomaterials on the mineral content of teeth. Eight of them are laboratory studies, and they found that silver nanomaterials prevented the demineralization of enamel and dentin under an acid or cariogenic biofilm challenge. The remaining three are clinical trials that reported that silver nanomaterials prevented and arrested caries in children. Conclusion: Silver nanoparticles have been used alone or with resin, glass ionomer, or fluoride for caries prevention. Silver nanomaterials inhibit the adhesion and growth of cariogenic bacteria. They also impede the demineralization of enamel and dentin.


Assuntos
Cárie Dentária/tratamento farmacológico , Cárie Dentária/prevenção & controle , Nanoestruturas/uso terapêutico , Prata/uso terapêutico , Cariostáticos/farmacologia , Cariostáticos/uso terapêutico , Cárie Dentária/microbiologia , Esmalte Dentário/efeitos dos fármacos , Dentina/efeitos dos fármacos , Humanos , Prata/farmacologia
5.
Lasers Med Sci ; 35(7): 1629-1636, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32382936

RESUMO

The aim of this study is to evaluate the ability of quantitative light-induced fluorescence (QLF) to monitor enamel caries lesions of different severity stages located on the occlusal surfaces of permanent teeth before and after treatment with resin infiltrant. Sixty extracted permanent teeth had one occlusal site selected and were categorized according to the International Caries Detection and Assessment System (ICDAS) criteria. The teeth were divided into three groups (n = 20): ICDAS 1, ICDAS 2, and ICDAS 3. The teeth were assessed by a trained examiner using QLF in two phases: (A) before and (B) after treatment with resin infiltrant. The caries lesions were evaluated using the following QLF parameters: area (mm2); ΔF, fluorescence loss (%); and ΔQ, fluorescence loss integrated over the lesion area (%*mm2). The resin infiltrant (Icon™) was applied on the occlusal surface following the manufacturer's recommendations. The teeth were then sectioned and prepared for polarized light microscopy analysis. The penetration of resin infiltrant was measured with ImageJ. The groups showed a statistically significant difference in all QLF parameters before and after caries infiltration, with the reduction of fluorescence values posttreatment (p < 0.05). Infiltrant penetration was observed in all groups, with a statistical difference between all groups (p < 0.05). The reduction in QLF parameters after resin infiltration suggests that QLF is able to monitor enamel caries lesions of different severity stages located on the occlusal surfaces of permanent teeth before and after treatment with resin infiltrant.


Assuntos
Cárie Dentária/diagnóstico , Esmalte Dentário/efeitos da radiação , Fluorescência Quantitativa Induzida por Luz , Resinas Sintéticas/farmacologia , Cárie Dentária/patologia , Esmalte Dentário/efeitos dos fármacos , Humanos , Dente/patologia
6.
J Appl Oral Sci ; 28: e20190163, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32236351

RESUMO

OBJECTIVE: This in vitro study evaluated the effect of commercial whitening dentifrices on erosive tooth wear (ETW) of bovine enamel samples, in comparison with commercial regular dentifrices. METHODOLOGY: Sixty bovine crowns were embedded in acrylic resin, polished and then had their baseline profile determined. They were randomly assigned to 5 groups (n=12/group), according to the type of commercial dentifrice to be tested: GI - Crest Anti-cavity Regular; GII - Crest 3D White; GIII - Colgate Total 12 Clean Mint; GIV - Colgate Optic White; GV - Placebo (negative control, fluoride-free dentifrice). The samples were submitted to daily erosive and abrasive challenges for 3 days. The erosive challenges were performed 3 times a day by immersing the specimens in 0.1% citric acid solution (pH 2.5) for 90 s. Each day after the first and last erosive challenges, the specimens were subjected to the abrasive challenge for 15 s, using a toothbrushing machine (Biopdi, São Carlos, SP, Brazil), soft toothbrushes and slurry (1:3 g/ml) of the tested toothpastes (1.5 N). The specimens were kept in artificial saliva between the challenges. The final profile was obtained and the ETW (µm) was calculated. Data were analyzed by Kruskal-Wallis and Dunn's tests (p<0.05). RESULTS: All dentifrices tested significantly reduced the enamel wear in comparison with the Placebo, except GIII. The median (95% CI) ETW was 1.35 (1.25-1.46)bc for GI, 1.17 (1.01-1.34)cd for GII, 1.36 (1.28-1.45)ab for GIII, 1.08 (1.04-1.14)d for GIV and 2.28 (2.18-2.39)a for GV. CONCLUSION: When dentifrices from the same manufacturer were compared, the whitening dentifrices led to similar or less wear than the regular ones.


Assuntos
Esmalte Dentário/efeitos dos fármacos , Clareadores Dentários/efeitos adversos , Erosão Dentária/induzido quimicamente , Cremes Dentais/efeitos adversos , Animais , Bovinos , Esmalte Dentário/química , Teste de Materiais , Estatísticas não Paramétricas , Propriedades de Superfície , Fatores de Tempo , Clareadores Dentários/química , Escovação Dentária/efeitos adversos , Cremes Dentais/química
7.
Acta Odontol Scand ; 78(5): 390-400, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32141357

RESUMO

Objective: Salivary flow rate exerts an essential impact on the development and progression of dental erosion. In this work, the experimental dental erosion in non-obese diabetic (NOD) mice with reduced salivary flow rate was induced, and the erosive effect of acidic drinks on their dentition was studied.Material and methods: Three acidic drinks (sports drink, cola light drink and sugar containing cola drink) were given to adult NOD mice (groups: N = 11) as the only drink for 6 weeks. Two control groups were included; wild type and NOD control (groups: N = 9). Experimental and control (water) teeth were dissected out and observed by scanning electron microscopy (SEM). Mandibular first molars were subsequently embedded in Epon, ground transversely, observed again by SEM, and the enamel thickness and tooth height were measured.Results: Mandibular molars were considerably more eroded than maxillary molars. The erosive process started at the top of the cusps and subsequently extended in the cervical, mesio-distal, and pulpal direction. Erosive lesions were evident in increased succession from sports drink, cola light to cola drink exposed mandibular molars, with the lingual tooth height being approximately 23%, 26%, and 37% lower, respectively, compared to the control. The lingual enamel was approximately 48% thinner in sports drink molars and 62% thinner in cola light molars. In cola drink molars, the lingual enamel was totally eroded, and significant erosion of dentine was evident.Conclusion: Reduced salivary flow, together with a high consumption of acidic drinks, results in severe erosion of NOD mice molars.


Assuntos
Bebidas/efeitos adversos , Bebidas Gaseificadas/efeitos adversos , Esmalte Dentário/efeitos dos fármacos , Glândulas Salivares/fisiopatologia , Erosão Dentária/induzido quimicamente , Animais , Esmalte Dentário/diagnóstico por imagem , Concentração de Íons de Hidrogênio , Camundongos , Camundongos Endogâmicos NOD , Microscopia Eletrônica de Varredura , Saliva/química
8.
BMC Oral Health ; 20(1): 49, 2020 02 11.
Artigo em Inglês | MEDLINE | ID: mdl-32046691

RESUMO

BACKGROUND: This study investigated the remineralisation effect of bioactive glass on artificial dentine caries. METHODS: Dentine disks with artificial caries were treated with bioactive glass (group BAG), casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) (group CPP-ACP), sodium fluoride glycerol (group F) or deionized water (group W). All disks were subjected to pH cycling for 28 days subsequently. The topography, microhardness and remineralisation depth of the dentine carious lesion were assessed by atomic force microscopy (AFM), microhardness testing and confocal laser scanning microscope (CLSM), respectively. RESULTS: AFM images indicated mineral depositions on the surface of the carious lesion in group BAG. The changes of Vickers hardness number (ΔVHN, mean ± SD) after pH cycling were 9.67 ± 3.60, 6.06 ± 3.83, 5.00 ± 2.19 and - 1.90 ± 2.09 (p < 0.001) in group BAG, group CPP-ACP, group F and group W, respectively. The remineralisation depth (mean ± SD) of the carious lesion in group BAG, group CPP-ACP, group F and group W were 165 ± 11 µm, 111 ± 11 µm, 75 ± 6 µm and 0 µm (p < 0.001), respectively. CONCLUSION: Bioactive glass possessed a promising remineralisation effect on artificial dentine caries and could be a therapeutic choice for caries management.


Assuntos
Caseínas/farmacologia , Cárie Dentária/tratamento farmacológico , Esmalte Dentário/efeitos dos fármacos , Dentina/efeitos dos fármacos , Vidro/química , Remineralização Dentária , Caseínas/uso terapêutico , Dureza , Humanos , Fluoreto de Sódio
9.
Rev. ADM ; 77(1): 22-27, ene.-feb. 2020. ilus, graf
Artigo em Espanhol | LILACS | ID: biblio-1087921

RESUMO

Introducción: Hoy en día, la mayoría de los pacientes requieren tratamiento de ortodoncia fija convencional, la cual utiliza procedimientos adhesivos afines con la superficie amelodentinaria. Aunque existen diferentes técnicas adhesivas que pueden ofrecer adecuados niveles de resistencia al desalojo, el uso del hipoclorito de sodio (NaClO) como agente desproteinizante no ha sido bien definido en materiales de cementación en aparatología ortodóncica convencional. Objetivo: El objetivo de este estudio fue determinar los niveles de resistencia al desalojo con pruebas de microtensión y análisis topográfico con microscopia electrónica de barrido. Material y métodos: 20 bloques de esmalte bovino fueron expuestos superficialmente a soluciones acondicionadoras (ácido ortofosfórico al 37%) con y sin agente desproteinizante (NaClO al 5.25%); finalmente, brackets individuales de ortodoncia fueron cementados con sistemas adhesivos y resinas compuestas de casas comerciales (Ormco y 3M). Las pruebas de resistencia al desalojo fueron realizadas con una máquina de fuerzas universales y el análisis topográfico con microscopia electrónica de barrido. Resultados: El uso del NaClO al 5.25% y la marca Ormco tuvieron estadísticamente los mejores niveles de adhesión que el grupo sin desproteinización y la casa comercial 3M, respectivamente. La topografía adamantina expuesta al agente desproteinizante mostró características de superficie relacionadas con el patrón de grabado tipo I. Conclusión: El uso de un agente desproteinizante (NaClO 5.25%) así como una marca comercial (Ormco) en particular podrían definir el aumento en los niveles de adhesión en superficies adamantinas con aparatología ortodóncica convencional (AU)


Introduction: Nowadays, patients require conventional fixed orthodontic treatment, which uses adhesive procedures related to the dentinal denture surface. Although there are different adhesive techniques that can offer adequate levels of bonding resistance, the use of NaClO as a deproteinizing agent has not been well defined in cementing materials in conventional orthodontic appliances. Objective: The objective of this study was to determine the levels of bond resistance using micro tension tests and topographic analysis with scanning electron microscopy. Material and methods: Twenty-blocks of bovine enamel were exposed superficially to conditioning solutions (37% orthophosphoric acid) with and without deproteinizing agent (5.25% NaClO); finally, individual orthodontic brackets were cemented with adhesive systems and resin composites of different trademarks (Ormco and 3M). Bonding resistance tests were performed with a universal force machine and topographic analysis was made with scanning electron microscopy. Results: The use of 5.25% NaClO and the Ormco brand had statistically better levels of adhesion than the group without deproteinization and the 3M brand, respectively. Adamantine topography exposed to the deproteinizing agent showed surface characteristics related to the type I etching pattern. Conclusion: The use of a deproteinizing agent (NaClO 5.25%) as well as a trademark (Ormco) in particular conditions could define the increase in adhesion levels on adamantine surfaces with conventional orthodontic appliances (AU)


Assuntos
Hipoclorito de Sódio/química , Condicionamento Ácido do Dente , Colagem Dentária , Braquetes Ortodônticos , Esmalte Dentário/efeitos dos fármacos , Ácidos Fosfóricos , Propriedades de Superfície , Resistência à Tração , Técnicas In Vitro , Microscopia Eletrônica de Varredura , Estudos Transversais , Análise Estatística , Resinas Compostas
10.
Arch Oral Biol ; 111: 104638, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31901573

RESUMO

OBJECTIVE: The purpose of this study was to investigate the effects of Lemon essential oil (LEO) and Limonene (LIM) in the progress of early caries. DESIGN: LEO and LIM were selected as experimental medicine, while sodium fluoride (NaF) and deionized water (DW) were positive and blank controls, respectively. Bovine incisors were used to establish enamel and dentin early caries models by demineralization method in vitro. Then specimens were subjected to pH cycling. Calcium and phosphate release of demineralizing solution were measured by an automatic biochemical analyzer; Surface microhardness tester and energy dispersive X-ray spectrometer were used to detect the surface microhardness recovery and calcium- phosphate ratio on tooth surface; Degraded collagen matrix by collagenase was investigated by assaying hydroxyproline. RESULTS: Calcium release of dentin demineralizing solution of LEO group was lower than DW group's and higher than NaF group's. Both of LEO and LIM groups, the surface microhardness recovery were significantly lower than those of NaF group, which were similar to DW group. Dentin surface calcium- phosphate ratio of LEO and LIM groups were lower than those of NaF group and higher than those of DW group. Hydroxyproline concentration in the remineralizing solution of LEO and LIM groups were lower than DW groups' and higher than NaF groups'. CONCLUSIONS: LEO and LIM have influence on the progress of dentin early caries, which can stabilize its structure by inhibiting collagen degradation. Meanwhile, these medicines may provide a new drug choice for the prevention and treatment of early root caries.


Assuntos
Cariostáticos/farmacologia , Cárie Dentária , Esmalte Dentário/efeitos dos fármacos , Limoneno/farmacologia , Óleos Voláteis/farmacologia , Óleos Vegetais/farmacologia , Animais , Bovinos , Concentração de Íons de Hidrogênio , Fluoreto de Sódio/farmacologia , Desmineralização do Dente , Remineralização Dentária
11.
Niger J Clin Pract ; 23(1): 110-115, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31929216

RESUMO

Objective: The aim of this study was to compare to effect of phototherapy and surface flattening after immediately bleaching on the shear bond strength to bleached enamel. Methods: Ninety-six human upper incisors were divided into 4 groups (n = 24). Group NB: no bleached, group P: phototherapy with YSGG laser, group F: 0.5 mm surface flattening, group PF: 0.5 mm surface flattening and phototherapy with Er;Cr:YSGG laser. Then, each group was assigned to 2 subgroups according to adhesive mode (n = 12) as; subgroup S (self-etching mode), subgroup T (total-etching mode) which are universal adhesives. All surface conditionings and restorations were performed with composite resin materials immediately after bleaching. Shear bond strength test was performed by using universal testing machine. The surfaces were also evaluated with SEM. The data were statistically analyzed with one-way ANOVA post-hoc Tukey tests. Results: The lowest SBS values were achieved in FS (13.72 ± 2.29) while the highest ones in PT (28.01 ± 6.81). However, the differences were not significant (P > 0.05). All surface conditioning methods provided SBS values similar to the control (P > 0.05). All subgroups of self-etching mode were significantly lower than their total-etching counterparts (P < 0.05). Conclusions: The present study showed that surface removal and phototherapy have a potential clinical application for eliminate to undesirable effect of bleaching treatment. Surface conditioning with either flattening and/or phototherapy may provide clinicians to restore bleached teeth at the same visit with bleaching and reduce chair-time.


Assuntos
Adesivos/farmacologia , Colagem Dentária/métodos , Esmalte Dentário/efeitos dos fármacos , Corrosão Dentária/métodos , Lasers de Estado Sólido/uso terapêutico , Fototerapia , Resistência ao Cisalhamento , Clareamento Dental , Condicionamento Ácido do Dente/métodos , Resinas Compostas/química , Materiais Dentários/química , Humanos , Teste de Materiais , Clareamento Dental/métodos
12.
Braz Oral Res ; 33: e111, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31800863

RESUMO

The aim of the present study was to evaluate the microtensile bond strength and the microleakage of a bulk-fill composite resin compared with a conventional incremental composite resin, in permanent molars and under cariogenic challenge using a Streptococcus mutans model. Permanent human third molars (n = 60) with an occlusal cavity of 5×3×2 mm were randomly allocated into four subgroups of restorative treatments: conventional composite resin with (n = 15) and without (n = 15) cariogenic challenge (Z350-E and Z350-C experimental and control groups, respectively), and bulk-fill composite resin with (n = 15) and without (n = 15) cariogenic challenge (Bulk Fill-E and Bulk Fill-C, respectively). Ten specimens from each subgroup were submitted to microtensile strength, and 5, to microleakage. The cariogenic challenge was conducted using the Streptococcus mutans strain (ATCC) for 7 days. The stickers obtained (1 × 1 × 2 mm) were submitted to a microtensile strength test, followed by classification of the fracture mode. Microleakage was performed using a scoring system. The data were analyzed by Kruskal-Wallis and Mann-Whitney tests (p < 0.05). Filtek Z350 XT resin presented higher microtensile bond strength than Bulk Fill resin without (19.02 ± 4.90 and 8.76 ± 3.94MPa, respectively; p < 0.001) and with cariogenic challenge (22.69 ± 7.86 and 13.31 ± 3.38MPa, respectively; p < 0.02). Z350-C and Bulk Fill-C resins presented a higher prevalence of mixed fractures (23 and 14%, respectively) in the specimens submitted to cariogenic challenge than those of the control groups, whereas microleakage was similar (p = 0.85). The conventional composite resin had higher microtensile bond strength than the bulk-fill resin, but both resin types had similar adhesion quality and microfiltration scores.


Assuntos
Biofilmes/efeitos dos fármacos , Resinas Compostas/química , Colagem Dentária/métodos , Streptococcus mutans/efeitos dos fármacos , Adesividade , Cárie Dentária , Esmalte Dentário/efeitos dos fármacos , Infiltração Dentária , Restauração Dentária Permanente/métodos , Humanos , Teste de Materiais , Reprodutibilidade dos Testes , Estatísticas não Paramétricas , Propriedades de Superfície , Resistência à Tração/efeitos dos fármacos , Fatores de Tempo
13.
Braz Oral Res ; 33: e114, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31800865

RESUMO

This in vitro study aimed to evaluate the effect of different toothpastes on dental enamel subjected to an erosive cycle with and without exposure to cigarette smoke. Bovine enamel specimens were randomly allocated into 12 groups (n = 12). For the in vitro simulation of smoking, half the groups underwent an exposure cycle of 20 cigarettes per day for 5 days. Subsequently, all groups were subjected to a 5-day erosion cycle intercalating demineralization (1 min; 1% citric acid; pH = 3.5) and treatment with toothpaste slurries (2 min) of NaF, SnF2, F/Sn/Chitosan, F/CaSiO3/Na3PO4, and F/bioactive glass. The control group was immersed in distilled water. Surface microhardness (SMH) was measured initially, after exposure to smoke, and after the erosive cycle, and %SMH was calculated. At the end of the experimental cycle, surface roughness, profilometry, and atomic force microscopy (AFM) were performed. SMH increased after exposure to cigarette smoke (p < 0.05). After the erosive cycle, there were no differences between the presence and absence of cigarette smoke exposure in SMH and roughness (p > 0.05). Besides increasing enamel SMH, cigarette smoke did not prevent enamel loss after the erosion cycle (p < 0.05). In profilometry, roughness and surface loss had the lowest values in the groups treated with SnF2 and F/Sn/Chitosan (p < 0.05). AFM showed lower mineral loss with F/CaSiO3/Na3PO4 and F/Sn/Chitosan. For all groups, except F/CaSiO3/Na3PO4, cigarette smoke resulted in higher enamel wear. F/Sn/Chitosan showed the best results against erosion.


Assuntos
Fumar Cigarros/efeitos adversos , Esmalte Dentário/efeitos dos fármacos , Erosão Dentária/etiologia , Erosão Dentária/prevenção & controle , Cremes Dentais/uso terapêutico , Animais , Compostos de Cálcio/uso terapêutico , Cariostáticos/uso terapêutico , Bovinos , Quitosana/uso terapêutico , Testes de Dureza , Humanos , Microscopia de Força Atômica , Valores de Referência , Reprodutibilidade dos Testes , Saliva/química , Silicatos/uso terapêutico , Propriedades de Superfície/efeitos dos fármacos , Fatores de Tempo , Fluoretos de Estanho/uso terapêutico , Desmineralização do Dente/induzido quimicamente , Desmineralização do Dente/prevenção & controle , Água/química
14.
BMC Oral Health ; 19(1): 247, 2019 11 14.
Artigo em Inglês | MEDLINE | ID: mdl-31727047

RESUMO

BACKGROUND: The aim of the present investigation was to evaluate enamel reduction efficiency, abrasive property decay, and enamel effects between oscillating mechanical and manual systems for interproximal enamel reduction (IPR). METHODS: Three oscillating strips and three manual strips were tested on twelve freshly extracted premolars blocked in an acrylic cylinder pot by means of a material testing machine. Each strip underwent one test of 8 cycles (30 s each). Both abrasive tracks and teeth surfaces were qualitative evaluated before and after IPR by means of SEM analysis. Efficiency and abrasive property decay of both IPR systems were investigated by the amount of enamel reduction within the eight-cycle testing. Independent t-test was used to evaluate differences in variables between the two systems. RESULTS: Mechanical IPR system showed higher efficiency in terms of enamel reduction (p < 0.005) when compared with manual IPR system (0.16 mm and 0.09 mm, respectively). Quantity of removed enamel decreased throughout the 8 cycles for both systems. Less presence of enamel debris and detachment of abrasive grains were observed on mechanical strips rather than manual strips. SEM analysis revealed more regular surface of teeth undergone mechanical IPR procedures. CONCLUSION: Oscillating diamond strips showed more controlled efficiency when compared with the manual IPR system leading to a more regular enamel surface.


Assuntos
Esmalte Dentário/efeitos dos fármacos , Diamante , Microabrasão do Esmalte/métodos , Dente Pré-Molar , Esmalte Dentário/patologia , Esmalte Dentário/ultraestrutura , Microabrasão do Esmalte/instrumentação , Humanos , Teste de Materiais , Microscopia Eletrônica de Varredura , Propriedades de Superfície
15.
Niger J Clin Pract ; 22(11): 1546-1552, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31719276

RESUMO

Objectives: The aim of this in vitro study was to evaluate the effects of casein phosphopeptides (CPP)-ACPF, NovaMin+ fluoride-containing toothpaste and Xylitol+ fluoride containing cream on demineralized areas on the enamel surface. Materials and Methods: A total of 100 enamel slab samples was prepared to investigate in the laboratory experiments. For this purpose, a total of 50 freshly extracted third molar teeth which completed root formation split into two portions in the mesiodistal direction. Enamel surfaces were immersed in a pH cycling protocol as described in the literature to simulate oral conditions for 9 days in order to evaluate the effect of test materials on the artificial enamel lesions. Then the remineralization agents were applied on the enamel surfaces, and we analyzed their effects. Results: We used Vickers Microhardness with the purpose of calculating the amount of lost or acquisition of minerals on the enamel surface qualitatively; inductively coupled plasma atomic emission spectroscopy (ICP-AES) to define the calcium and phosphorus ions that dissolved in acid. One-way ANOVA and Tukey's T Post-Hoc tests were performed to distinguish significant differences among groups (P < 0.05). Conclusions: Remineralization was provided in all treated groups, according to the data obtained from all tests. NovaMin was more effective in increasing acid resistance. It was also found that all three experimental groups were effective in increasing the surface hardness, but CPP-ACPF and NovaMin are more effective than Xylitol. However, there was no statistically significant difference between the experimental groups.


Assuntos
Esmalte Dentário/efeitos dos fármacos , Fluoretos Tópicos/uso terapêutico , Fluoretos/uso terapêutico , Vidro , Desmineralização do Dente/tratamento farmacológico , Remineralização Dentária/métodos , Cremes Dentais , Xilitol/uso terapêutico , Cálcio , Caseínas/uso terapêutico , Fluoretos/farmacologia , Dureza , Humanos , Cremes Dentais/farmacologia , Xilitol/administração & dosagem , Xilitol/farmacologia
16.
Braz Oral Res ; 33: e101, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31778472

RESUMO

The aim of this study was to evaluate the influence of different calcium phosphates (CaPs) on the physical, biological, and remineralizing properties of experimental resin-based sealants (RBSs). Triethylene-glycol dimethacrylate (90wt%) and bisphenol A-glycidyl methacrylate (10wt%) were used to produce resin-based sealants. Hydroxyapatite (SHAp), α-tricalcium phosphate (Sα-TCP) and octacalcium phosphate (SOCP) were added to the sealants in a 10wt% concentration. One group without CaPs was used as the control group (SCG). The degree of conversion (DC) was assessed with Fourier-transformed infrared spectroscopy, whereas cytotoxicity was tested with the HaCaT keratinocyte cell line. The ultimate tensile strength (UTS) was used to assess the mechanical strength of the experimental RBSs. Sealed enamel was used for colorimetric assay. Mineral deposition was assessed with Raman spectroscopy after 7, 14, and 28 days of sample immersion in artificial saliva. Scanning electron microscopy was used to analyze the surface morphology after 28 days of immersion. The addition of 10wt% of fillers significantly reduced the DC of sealants. SOCP groups showed reduced cell viability. Higher UTS was found for Sα-TCP and SHAp. The color analysis showed that SGC and demineralized teeth presented higher mismatches with the sound tissue. Mineral deposition was observed for SHAp and Sα-TCP after 7 days, with increased phosphate content and mineral deposits for SHAp after 28 days. RBS with the addition of 10% HAp promoted increased mineralization in vitro after 28 days, and did not affect cell viability, DC, mechanical properties, or RBS color in the enamel.


Assuntos
Fosfatos de Cálcio/química , Durapatita/química , Minerais/química , Selantes de Fossas e Fissuras/química , Resinas Sintéticas/química , Animais , Fosfatos de Cálcio/toxicidade , Bovinos , Linhagem Celular , Colorimetria , Esmalte Dentário/química , Esmalte Dentário/efeitos dos fármacos , Durapatita/toxicidade , Humanos , Teste de Materiais , Microscopia Eletrônica de Varredura , Selantes de Fossas e Fissuras/toxicidade , Valores de Referência , Reprodutibilidade dos Testes , Resinas Sintéticas/toxicidade , Saliva Artificial/química , Análise Espectral Raman , Propriedades de Superfície , Resistência à Tração , Fatores de Tempo
17.
Braz Oral Res ; 33: e120, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31778475

RESUMO

The objective of this study is to compare the resin-enamel bond strength (mµSBS), in-situ degree of conversion (DC), and the enamel-etching pattern (SEM/EDX) of universal adhesive systems when applied to sound and fluorotic enamel. Ninety-eight human molars were sectioned into 4 parts and divided into 24 groups according to 1) enamel surface (sound or fluorotic enamel), 2) adhesive system (Clearfil Universal Bond [CUB], Futurabond U [FBU], iBond Universal [IBU], and Scotchbond Universal [SBU]), and 3) application mode (etch-and-rinse [ER], active self-etch [Active-SE], and passive self-etch [Passive-SE]). Specimens were stored at 37 °C, for 24 hours and tested at 1.0 mm/min (µSBS). Enamel-resin interfaces were evaluated for in-situ DC. The enamel-etching pattern was evaluated under a SEM/EDX. Data from mµSBS and in-situ DC was analyzed using a three-way ANOVA and Tukey's test at 5 % level of significance. For all adhesives, the ER resulted in a statistically significant higher mean mµSBS than the passive-SE in both substrates (p < 0.001). For all adhesives, active-SE resulted in mean mµSBS (p > 0.31) and in-situ DC (p > 0.45) that were statistically similar to those obtained with the ERs in both substrates. A statistically significant, higher mean mµSBS and in-situ DC were obtained in sound enamel (p < 0.001) than in fluorotic enamel. In general, SBU showed higher mean values for mµSBS and in-situ DC compared to those of CUB and IBU (p < 0.001). ER and active-SE showed the deepest enamel-etching pattern in both substrates. A higher amount of fluor was observed in fluorotic enamel. The active application of universal adhesives in the SE-mode may be a viable alternative to increase the adhesive properties in sound and fluorotic enamel.


Assuntos
Colagem Dentária/métodos , Esmalte Dentário/química , Esmalte Dentário/efeitos dos fármacos , Fluorose Dentária , Cimentos de Resina/química , Análise de Variância , Humanos , Teste de Materiais , Microscopia Eletrônica de Varredura , Distribuição Aleatória , Valores de Referência , Reprodutibilidade dos Testes , Resistência ao Cisalhamento/efeitos dos fármacos , Espectrometria por Raios X , Análise Espectral Raman , Propriedades de Superfície/efeitos dos fármacos
18.
Braz Oral Res ; 33: e098, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31664361

RESUMO

The aim was to evaluate the effect of 2% grape seed extract (GSE) containing phosphoric acid (PhA) on the bond strength to enamel and dentin. The control group was 37% PhA. The following three PhA formulations with 2% GSE and 20% ethanol were obtained: GSE5 = 5% PhA; GSE10 = 10% PhA; and GSE20 = 20% PhA. The enamel and dentin surfaces of molars were etched with the acid solutions, followed by Scotchbond Multi-Purpose adhesive and composite resin application. The tensile bond strength (TBS) test evaluated the bond to enamel after 24 h, and the microtensile bond strength (µTBS) test evaluated the bond to dentin after 24 h and 12-month water storage. Etched enamel and dentin were observed by scanning electron microscopy (SEM) and atomic force microscopy (AFM), respectively. The TBS data were submitted to one-way ANOVA, while µTBS data were submitted to two-way ANOVA and Tukey's test (α = 0.05). The TBS (MPa) to enamel did not significantly differ among the control (48.1 ± 15.7), GSE5 (46.1 ± 9.6), GSE10 (49.8 ± 13.6) and GSE20 (44.1 ± 11.9) groups (p = 0.537). The µTBS (MPa) to dentin of the control (28.4 ± 14.4) and GSE20 (24.1 ± 8.1) groups were significantly higher than those of the GSE5 (16.8 ± 7.4) and GSE10 (17.5 ± 6.6) groups at 24 h (p < 0.006). After 12-month storage, only GSE5 (21.0 ± 7.8) and GSE10 (17.6 ± 8.0) did not show significantly decreased µTBS (p > 0.145). SEM micrographs showed a shallower enamel etching pattern for GSE5. AFM images showed the formation of collagenous globular structures for GSE5 and GSE10. The different acid solutions did not influence the TBS to enamel, and the µTBS to dentin was stable over time when dentin was etched with GSE5 and GSE10.


Assuntos
Condicionamento Ácido do Dente/métodos , Colagem Dentária/métodos , Esmalte Dentário/efeitos dos fármacos , Dentina/efeitos dos fármacos , Extrato de Sementes de Uva/química , Ácidos Fosfóricos/química , Adolescente , Adulto , Análise de Variância , Esmalte Dentário/química , Dentina/química , Humanos , Teste de Materiais , Microscopia de Força Atômica , Microscopia Eletrônica de Varredura , Valores de Referência , Reprodutibilidade dos Testes , Estatísticas não Paramétricas , Propriedades de Superfície/efeitos dos fármacos , Resistência à Tração , Fatores de Tempo , Adulto Jovem
19.
Braz Oral Res ; 33: e0088, 2019 Sep 09.
Artigo em Inglês | MEDLINE | ID: mdl-31531566

RESUMO

The main of the study was quantify the effect of two ceramics with two underlying resin cements on apparent fluorescence levels. Buccal surfaces of two bovine incisors were ground flat producing one enamel and one dentin substrate. The veneers were fabricated (0.5 and 1.0 mm thickness) using two ceramics (IPSe.max Press and IPSe.max Zirpress, Ivoclar Vivadent). Veneers were cemented using either light-cured (Variolink II, Ivoclar Vivadent) or self-adhesive dual (Rely X U200, 3M ESPE) cement. The layered Control group materials had no cement application. Semi-quantitative fluorescence image analysis (Matlabs software, Matworks) involved processing the images as captured under each daylight (DL, Gretagmacbeth) and ultraviolet illuminants (UVA, Sylvania) within a neutral-gray lightbox (Macbeth Spectral Light). Statistical analysis of the quantitative fluorescence values was performed using two-way ANOVA and Tukey's test (p < 0.05). The e.max Zirpress on the dentin substrate produced greater fluorescence (p < 0.05) when subjected to UV illumination and more fluorescence (p < 0.05) than e.max Press in both cement groups. Light-cured cement produced higher (p < 0.05) fluorescence than the dual-cement with e.max Press on enamel under UV illumination. The fluorescence for e.max Press on the dentin substrate was greater (p < 0.05) than for e.max Zirpress using dual self-adhesive cement subjected to daylight illumination. Thus, it is possible to conclude that the combination of ceramic and cement produce definite, significant effects on the apparent fluorescence, vital quality for restorative dentistry.


Assuntos
Cerâmica/química , Facetas Dentárias , Imagem Óptica/métodos , Cimentos de Resina/química , Animais , Bovinos , Esmalte Dentário/química , Esmalte Dentário/efeitos dos fármacos , Dentina/química , Dentina/efeitos dos fármacos , Luz , Teste de Materiais , Valores de Referência , Reprodutibilidade dos Testes , Propriedades de Superfície
20.
Braz Oral Res ; 33: e037, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31508726

RESUMO

Iontophoresis is a noninvasive technique, based on the application of a constant low-intensity electric current to facilitate the release of a variety of drugs, whether ionized or not, through biological membranes. The objective of this research was to evaluate the effect of iontophoresis using different electric current intensities on the uptake of fluoride in dental enamel with artificial caries lesions. In this in vitro operator-blind experiment, bovine enamel blocks (n = 10/group) with caries-like lesions and predetermined surface hardness were randomized into 6 groups: placebo gel without fluoride applied with a current of 0.8 mA (negative control), 2% NaF gel without application of any current, and 2% NaF gel applied with currents of 0.1, 0.2, 0.4 and 0.8 mA. Cathodic iontophoresis was applied for 4 min. The concentration of loosely bound fluoride (calcium fluoride) and firmly bound fluoride (fluorapatite) was determined. The results were analyzed by the nonparametric Kruskal-Wallis and Dunn tests. Iontophoresis at 0.8 mA, combined with the application of fluoridated gel (2% NaF), increased fluoride uptake in enamel with caries-like lesions, as either calcium fluoride or fluorapatite.


Assuntos
Cariostáticos/farmacologia , Cárie Dentária , Esmalte Dentário/efeitos dos fármacos , Fluoretos/farmacologia , Iontoforese/métodos , Animais , Apatitas/análise , Fluoreto de Cálcio/análise , Bovinos , Modelos Animais de Doenças , Eletricidade , Dureza/efeitos dos fármacos , Distribuição Aleatória , Propriedades de Superfície/efeitos dos fármacos , Resultado do Tratamento
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