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1.
J Med Life ; 14(1): 68-74, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33767788

RESUMO

This article highlights the results of a study of blood parameters in animals with simulated necrotizing ulcerative gingivitis and compares them, under the same conditions, with animals that received local treatment with a developed complex of antioxidant drugs. Following the work tasks, the nature of changes in the state of the antioxidant - prooxidant system and their influence on quantitative and functional indicators of markers of inflammatory intensity was analyzed and investigated during the pathological process in the background and without treatment with a developed complex. This work shows the changes of malonic dialdehyde concentration as an indicator of lipid peroxidation intensity in experimental animals, the level of catalase activity in the blood of animals, and antioxidant-prooxidant balance in the dynamics of necrotizing ulcerative gingivitis.


Assuntos
Antioxidantes/metabolismo , Gengivite Ulcerativa Necrosante/sangue , Animais , Biomarcadores/sangue , Catalase/sangue , Humanos , Peroxidação de Lipídeos , Masculino , Malondialdeído/sangue , Oxirredução , Coelhos , Espécies Reativas de Oxigênio/sangue
2.
Medicine (Baltimore) ; 100(11): e25104, 2021 Mar 19.
Artigo em Inglês | MEDLINE | ID: mdl-33725987

RESUMO

ABSTRACT: Our aim in this study was to investigate the relationship between serum ischemia modified albumin (IMA) levels with oxidative stress parameters [protein carbonyl (PCO), advanced protein oxidation products (AOPPs), malondialdehyde (MDA), total nitric oxide (NOx), prooxidant-antioxidant balance (PAB), and ferric reducing of antioxidant power (FRAP)] in breast cancer (BC) and colon cancer (CC).In total, 90 patients undergoing surgical treatment for BC (n = 45) or CC (n = 45) and 35 healthy controls were included in this cross-sectional study.The serum PCO, AOPPs, MDA, NOx, PAB, and IMA levels were all statistically significantly higher in the cancer patients than in the control group. MDA, NOx, and PAB levels were significantly lower in the BC group than in the CC group. FRAP values were statistically significantly lower in both the CC group and the BC group compared to the control. IMA showed a weak positive correlation with CA-19.9 (r = 0.423 P = .007) but a moderate positive correlation with tumor size in the CC group. IMA showed a positive correlation with metastasis, grade, and HER2 and a negative correlation with ER and PR in the BC group.Oxidative stress is a key player in the development of solid malignancies. Cancer development is a multistage process, and oxidative stress caused by the production of ROS/RNS in the breast and colon may predispose individuals to BC and CC. Patients with BC and CC had an impaired oxidative/antioxidant condition that favored oxidative stress. The ROC analysis indicated that IMA sensitivity above 80% could be used as a secondary biomarker in diagnosis.


Assuntos
Neoplasias da Mama/sangue , Neoplasias do Colo/sangue , Estresse Oxidativo , Espécies Reativas de Nitrogênio/sangue , Espécies Reativas de Oxigênio/sangue , Produtos da Oxidação Avançada de Proteínas/sangue , Antioxidantes/metabolismo , Biomarcadores Tumorais/sangue , Neoplasias da Mama/patologia , Estudos de Casos e Controles , Neoplasias do Colo/patologia , Estudos Transversais , Feminino , Compostos Férricos/sangue , Humanos , Masculino , Malondialdeído/sangue , Pessoa de Meia-Idade , Gradação de Tumores/métodos , Estadiamento de Neoplasias/métodos , Óxido Nítrico/sangue , Oxirredução , Carbonilação Proteica , Curva ROC , Albumina Sérica/análise
3.
Ann Hematol ; 100(4): 891-901, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33388858

RESUMO

Curcuminoids, polyphenol compounds in turmeric, possess several pharmacological properties including antioxidant, iron-chelating, and anti-inflammatory activities. Effects of curcuminoids in thalassemia patients have been explored in a limited number of studies using different doses of curcuminoids. The present study aims to evaluate the effects of 24-week curcuminoids supplementation at the dosage of 500 and 1000 mg/day on iron overload, oxidative stress, hypercoagulability, and inflammation in non-transfused ß-thalassemia/Hb E patients. In general, both curcuminoids dosages significantly lowered the levels of oxidative stress, hypercoagulability, and inflammatory markers in the patients. In contrast, reductions in iron parameter levels were more remarkable in the 1000 mg/day group. Subgroup analysis revealed that a marker of hypercoagulability was significantly decreased only in patients with baseline ferritin ≤ 1000 ng/ml independently of curcuminoids dosage. Moreover, the alleviation of iron loading parameters was more remarkable in patients with baseline ferritin > 1000 ng/ml who receive 1000 mg/day curcuminoids. On the other hand, the responses of oxidative stress markers were higher with 500 mg/day curcuminoids regardless of baseline ferritin levels. Our study suggests that baseline ferritin levels should be considered in the supplementation of curcuminoids and the appropriate curcuminoids dosage might differ according to the required therapeutic effect. Thai Clinical Trials Registry (TCTR): TCTR20200731003; July 31, 2020 "retrospectively registered".


Assuntos
Diarileptanoides/uso terapêutico , Suplementos Nutricionais , Hemoglobina E/genética , Hemoglobinopatias/tratamento farmacológico , Inflamação/tratamento farmacológico , Sobrecarga de Ferro/tratamento farmacológico , Trombofilia/tratamento farmacológico , Adolescente , Adulto , Biomarcadores , Proteínas Sanguíneas/análise , Citocinas/sangue , Diarileptanoides/administração & dosagem , Diarileptanoides/farmacologia , Relação Dose-Resposta a Droga , Feminino , Ferritinas/sangue , Hemoglobinopatias/sangue , Hemoglobinopatias/complicações , Hemoglobinopatias/genética , Heterozigoto , Humanos , Inflamação/sangue , Inflamação/etiologia , Sobrecarga de Ferro/etiologia , Masculino , Malondialdeído/sangue , Pessoa de Meia-Idade , Estresse Oxidativo/efeitos dos fármacos , Espécies Reativas de Oxigênio/sangue , Estudos Retrospectivos , Trombofilia/sangue , Trombofilia/etiologia , Adulto Jovem , Globinas beta/genética , Talassemia beta/sangue , Talassemia beta/complicações , Talassemia beta/tratamento farmacológico , Talassemia beta/genética
4.
PLoS One ; 15(9): e0239516, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32956398

RESUMO

Intense dance training leads to inflammation, which may impair the health and performance of the practitioners. Herein, we evaluate the effect of a single street dancing class on the profile of muscle enzymes, lymphocyte activation, and cell surface CD62L expression. We also investigated the correlation between muscle enzymes, adhesion molecules, and lymphocyte activation in dancers. Fifteen male participants (mean ± standard error: age 22.4 ± 1.08 years, body mass index 24.8 ± 0.69 kg/m2, body fat 12.3 ± 1.52%), who were amateur dancers, had blood samples collected previously and subsequent to a high-intensity street dance class. After the class, dancers showed an increase in total lymphocyte count (2.0-fold), creatine kinase (CK)-NAC (4.87%), and CK-MB (3.36%). We also observed a decrease (2.5-fold) in reactive oxygen species (ROS) produced by lymphocytes, under phorbol myristate acetate-stimulated environments. Following the dance class, CD62L expression in lymphocytes decreased (51.42%), while there was a negative correlation between the intensity of the exercise and CD62L expression (r = -0.73; p = 0.01). Lymphocytes were less responsive to stimuli after a single bout of street dancing, indicating transient immunosuppression.


Assuntos
Dança/fisiologia , Selectina L/análise , Ativação Linfocitária , Creatina Quinase Forma MB/sangue , Dança/educação , Frequência Cardíaca , Humanos , Inflamação , Ativação Linfocitária/efeitos dos fármacos , Contagem de Linfócitos , Masculino , Espécies Reativas de Oxigênio/sangue , Acetato de Tetradecanoilforbol/farmacologia , Adulto Jovem
5.
Mem Inst Oswaldo Cruz ; 115: e200082, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32935750

RESUMO

Respiratory failure (RF) is the main cause of hospital admission in HIV/AIDS patients. This study assessed comorbidities and laboratory parameters in HIV/AIDS inpatients with RF (N = 58) in relation to those without RF (N = 36). Tuberculosis showed a huge relative risk and platelet counts were slightly higher in HIV/AIDS inpatients with RF. A flow cytometry assay for reactive oxygen species (ROS) showed lower levels in platelets of these patients in relation to the healthy subjects. However, when stimulated with adrenaline, ROS levels increased in platelets and platelet-derived microparticles of HIV/AIDS inpatients, which may increase the risk of RF during HIV and tuberculosis (HIV-TB) coinfection.


Assuntos
Micropartículas Derivadas de Células/metabolismo , Infecções por HIV/sangue , HIV/imunologia , Espécies Reativas de Oxigênio/sangue , Insuficiência Respiratória/complicações , Fármacos Anti-HIV/uso terapêutico , Biomarcadores/sangue , Plaquetas , Citometria de Fluxo , Infecções por HIV/complicações , Infecções por HIV/tratamento farmacológico , Humanos , Insuficiência Respiratória/sangue
6.
Medicine (Baltimore) ; 99(31): e21332, 2020 Jul 31.
Artigo em Inglês | MEDLINE | ID: mdl-32756118

RESUMO

Biliary atresia (BA) is a devastating cholestatic disorder of infants that presents during the first several months after birth due to an idiopathic obstruction to the bile flow. Without prompt diagnosis, Kasai portoenterostomy, and deliberate follow-ups, the resulting cholestasis leads to progressive hepatic failure. Oxidative stress is an abnormal phenomenon inside cells or tissues caused by a disturbance in the reactive oxygen species (ROS). We aimed to measure perioperative ROS in BA patients.Data are presented as median (25th, 75th percentiles). We evaluated 15 BA patients (age 55 [48, 69] days) and measured ROS; serum superoxide dismutase (SOD), urinary 8-iso prostaglandin F2α (8-iso-PGF2α) and 8-hydroxy-2'-deoxyguanosine (8-OHdG) preoperatively and 30 days later to compare values with serum liver function tests and histologic grades of liver cholestasis. For compared BA patients, 4 normal subjects as control group (age 55 [27, 75] days) measured ROS and serum liver function tests.In BA patients, the preoperative serum SOD was 6.1 IU/mL (4.7, 7.2), urinary 8-iso-PGF2α was 1969 pg/mg Cre (1697, 2374), and urinary 8-OHdG was 37.1 ng/mg Cre (33.1, 53.7). At the postoperative day 30, the serum SOD was 5.2 IU/mL (4.2, 6.7), urinary 8-iso-PGF2α was 1761 pg/mg Cre (1256, 3036), and urinary 8-OHdG was 42.1 ng/mg Cre (29.65, 72.64). In ROS, there were no significant differences between the 2 periods. In control group, urinary 8-iso-PGF2α was significantly lower than that in preoperative BA patient group. However, other ROS were not significant differences between control group and BA patient group. The concentration of urinary 8-iso-PGF2α was positively correlated with total bilirubin and direct bilirubin levels (preoperatively: r = 0.6921, P = .0042 and r = 0.6639, P = .007, postoperatively: r = 0.6036, P = .0172 and r = 0.6464, P = .0092, respectively). The preoperative ROS were not correlated with histologic grades of liver cholestasis. Various factors such as liver inflammation, lipid malabsorption, and tissue disorders due to jaundice might affect the antioxidant activity and elevated urinary 8-iso-PGF2α. However, at least until 30 days later, urinary 8-OHdG as oxidative DNA damage might persist after the operation whether the cholestasis improved or not.


Assuntos
Atresia Biliar/cirurgia , Espécies Reativas de Oxigênio/sangue , Atresia Biliar/sangue , Bilirrubina/metabolismo , Biomarcadores/sangue , Biomarcadores/urina , Estudos de Casos e Controles , Feminino , Humanos , Lactente , Testes de Função Hepática , Masculino , Estudos Retrospectivos
7.
Clin Exp Hypertens ; 42(7): 661-668, 2020 Oct 02.
Artigo em Inglês | MEDLINE | ID: mdl-32476477

RESUMO

OBJECTIVE: Evidence has shown that the ACE2/Ang (1-7)/Mas axis plays an important role in the control of hypertension. Thus, we hypothesized that chemical renal denervation (RDN) could reduce blood pressure by regulating the ACE2/Ang (1-7)/Mas axis in spontaneously hypertensive rats. METHODS: Twelve rats were randomly divided into sham group and chemical RDN group. All the rats were sacrificed 4 weeks later. Plasma samples were collected to measure the renin-angiotensin system (RAS) activities and reactive oxygen species levels by radioimmunoassay, chromatometry and ELISA. Paraventricular nucleus (PVN) tissues were collected to examine the expression of the components of the ACE2/Ang (1-7)/Mas axis by western bolt and immunofluorescence. RESULTS: The systolic blood pressure (169.33 ± 7.50 vs 182.67 ± 7.00 mmHg, p < .05) and the diastolic blood pressure (97.50 ± 4.68 vs 109.33 ± 4.41 mmHg, p < .05) in the RDN group were obviously lower than the baseline levels, whereas the opposite results were observed in the sham group. The RDN group exhibited a significant reduction in the plasma ROS (91.59 ± 13.12 vs 72.34 ± 11.76 U/ml, p < .05) and NADPH oxidase (171.86 ± 1.14 vs 175.75 ± 1.74 nmol/ml, p < .001) compared with the sham group, while the plasma eNOS (3.47 ± 0.42 vs 2.49 ± 0.51 U/ml, p < .05) and NO (55.92 ± 8.10 vs 43.53 ± 4.58 µmol/L, p < .05) were increased. The expression of the components of the ACE2/Ang (1-7)/Mas axis was upregulated while the expression of the components of the ACE/Ang II/AT1 R axis was downregulated in the plasma and PVN in the RDN group. CONCLUSION: Our findings suggested that the reduction in blood pressure was regulated by chemical RDN-induced upregulation of the components of the ACE2/Ang (1-7)/Mas axis.


Assuntos
Angiotensina I/metabolismo , Pressão Sanguínea , Fragmentos de Peptídeos/metabolismo , Peptidil Dipeptidase A/metabolismo , Proteínas Proto-Oncogênicas/metabolismo , Receptores Acoplados a Proteínas-G/metabolismo , Simpatectomia Química , Animais , Hipertensão/fisiopatologia , Masculino , NADPH Oxidases/sangue , Óxido Nítrico/sangue , Óxido Nítrico Sintase Tipo III/sangue , Núcleo Hipotalâmico Paraventricular/metabolismo , Distribuição Aleatória , Ratos , Ratos Endogâmicos SHR , Espécies Reativas de Oxigênio/sangue , Artéria Renal/inervação , Sistema Renina-Angiotensina , Regulação para Cima
8.
ACS Infect Dis ; 6(7): 1558-1562, 2020 07 10.
Artigo em Inglês | MEDLINE | ID: mdl-32463221

RESUMO

Higher rates of serious illness and death from coronavirus SARS-CoV-2 (COVID-19) infection among older people and those who have comorbidities suggest that age- and disease-related biological processes make such individuals more sensitive to environmental stress factors including infectious agents like coronavirus SARS-CoV-2. Specifically, impaired redox homeostasis and associated oxidative stress appear to be important biological processes that may account for increased individual susceptibility to diverse environmental insults. The aim of this Viewpoint is to justify (1) the crucial roles of glutathione in determining individual responsiveness to COVID-19 infection and disease pathogenesis and (2) the feasibility of using glutathione as a means for the treatment and prevention of COVID-19 illness. The hypothesis that glutathione deficiency is the most plausible explanation for serious manifestation and death in COVID-19 patients was proposed on the basis of an exhaustive literature analysis and observations. The hypothesis unravels the mysteries of epidemiological data on the risk factors determining serious manifestations of COVID-19 infection and the high risk of death and opens real opportunities for effective treatment and prevention of the disease.


Assuntos
Anti-Inflamatórios/farmacologia , Anticoagulantes/farmacologia , Antioxidantes/farmacologia , Antivirais/farmacologia , Betacoronavirus/efeitos dos fármacos , Infecções por Coronavirus/mortalidade , Infecções por Coronavirus/patologia , Glutationa/deficiência , Glutationa/farmacologia , Pneumonia Viral/mortalidade , Pneumonia Viral/patologia , Adulto , Anti-Inflamatórios/sangue , Anticoagulantes/sangue , Antivirais/sangue , Betacoronavirus/fisiologia , Infecções por Coronavirus/sangue , Infecções por Coronavirus/virologia , Suscetibilidade a Doenças , Estudos de Viabilidade , Feminino , Glutationa/sangue , Humanos , Pessoa de Meia-Idade , Estresse Oxidativo/efeitos dos fármacos , Pandemias , Pneumonia Viral/sangue , Pneumonia Viral/virologia , Prognóstico , Espécies Reativas de Oxigênio/sangue , Fatores de Risco , Índice de Gravidade de Doença , Replicação Viral/efeitos dos fármacos , Deficiência de Vitamina D/complicações
9.
Zhonghua Gan Zang Bing Za Zhi ; 28(3): 247-253, 2020 Mar 20.
Artigo em Chinês | MEDLINE | ID: mdl-32306658

RESUMO

Objective: To investigate the application value of reactive oxygen species (ROS) and adiponectin (ADPN) in the judgment of liver inflammation in chronic hepatitis B virus infection combined with nonalcoholic fatty liver disease (NAFLD). Methods: A total of 159 cases with NAFLD (21 cases), chronic hepatitis B virus infection (57 cases), and chronic hepatitis B virus infection combined with NAFLD (81 cases) were collected between June 2016 to December 2018, and the visited patients diagnosis were confirmed by histopathological examination of the liver. ROS and ADPN level retained in serum was determined by enzyme-linked immunosorbent assay. Histopathological examination of liver tissue was used as the gold standard to discuss the diagnostic value of the serum in patients with chronic hepatitis B virus infection combined with NAFLD for the occurrence of nonalcoholic steatohepatitis. One-way analysis of variance was used for the comparison among multiple groups, and LSD-t test was used for pairwise comparison between groups. Measurement data for non-normal distributions were expressed as M (P25, P75). Comparisons between groups were performed using the Mann-Whitney U or Kruskal-Wallis H test. Chi-square test was used to compare the count data between groups. Correlation analysis was performed using Spearman correlation analysis. Histopathological grouping of liver tissue was used as the gold standard, and the area under the receiver operating characteristic curve was used to evaluate the diagnostic efficacy of the regression formula. Results: (1) In patients with chronic hepatitis B virus infection combined with NAFLD, the levels of ROS in the non-hepatic steatosis group and the mild hepatic steatosis group were significantly lower than those in the moderate and severe hepatic steatosis group, while the ADPN level in the non-hepatic steatosis group was significantly higher than liver steatosis group, P < 0.05. (2) The results of correlation analysis showed that ROS was significantly correlated with NAS score, change in the degree of fatty liver and lobular inflammation (all P < 0.05).There was a significant negative correlation between ADPN and the change in the degree of fatty liver (P < 0.05). (3) Logistic regression analysis results showed that the diagnostic formula for chronic hepatitis B virus infection combined with nonalcoholic steatohepatitis was 0.02 × controlled attenuation index + 0.584 × white blood cells/10(9) + 0.587 × ROS-10.982. The area under receiver operating characteristic curve of the subject was = 0.896. The sensitivity, specificity, positive and negative predictive value were 97.1%, 71.2%, 64.2%, and 97.9%. Conclusion: ADPN and ROS have certain reference value in differentiating the change in the degree of fatty liver and inflammation in chronic hepatitis B virus infection combined with NAFLD and the diagnostic formula has higher application value in the diagnosis and exclusion of chronic hepatitis B virus infection combined with nonalcoholic steatohepatitis.


Assuntos
Adiponectina/sangue , Hepatite B Crônica/sangue , Hepatopatia Gordurosa não Alcoólica/sangue , Hepatopatia Gordurosa não Alcoólica/virologia , Espécies Reativas de Oxigênio/sangue , Biópsia , Vírus da Hepatite B , Hepatite B Crônica/complicações , Humanos , Fígado
10.
An Bras Dermatol ; 95(3): 314-319, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32307203

RESUMO

BACKGROUND: Although not fully understood, oxidative stress has been implicated in the pathogenesis of different autoimmune diseases such as systemic sclerosis. Accumulating evidence indicates that oxidative stress can induce mitochondrial DNA (mtDNA) damage and variations in mtDNA copy number (mtDNAcn). OBJECTIVE: The aim of this study was to explore mtDNAcn and oxidative DNA damage byproducts in peripheral blood of patients with systemic sclerosis and healthy controls. METHODS: Forty six patients with systemic sclerosis and forty nine healthy subjects were studied. Quantitative real-time PCR used to measure the relative mtDNAcn and the oxidative damage (oxidized purines) of each sample. RESULTS: The mean mtDNAcn was lower in patients with systemic sclerosis than in healthy controls whereas the degree of mtDNA damage was significantly higher in cases as compared to controls. Moreover, there was a negative correlation between mtDNAcn and oxidative DNA damage. STUDY LIMITATIONS: The lack of simultaneous analysis and quantification of DNA oxidative damage markers in serum or urine of patients with systemic sclerosis and healthy controls. CONCLUSION: These data suggest that alteration in mtDNAcn and increased oxidative DNA damage may be involved in the pathogenesis of systemic sclerosis.


Assuntos
Variações do Número de Cópias de DNA , Dano ao DNA , DNA Mitocondrial/sangue , DNA Mitocondrial/genética , Estresse Oxidativo/genética , Escleroderma Sistêmico/sangue , Escleroderma Sistêmico/genética , Adulto , Estudos de Casos e Controles , Eletroforese em Gel de Ágar , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Espécies Reativas de Oxigênio/sangue , Reação em Cadeia da Polimerase em Tempo Real , Valores de Referência , Estatísticas não Paramétricas
11.
Physiol Biochem Zool ; 93(2): 90-96, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32011970

RESUMO

Oxidative stress, the imbalance of reactive oxygen species and antioxidant capacity, may cause damage to biomolecules pivotal for cellular processes (e.g., DNA). This may impair physiological performance and, therefore, drive life-history variation and aging rate. Because aerobic metabolism is supposed to be the main source of such oxidative risk, the rate of oxygen consumption should be positively associated with the level of damage and/or antioxidants. Empirical support for such relationships remains unclear, and recent considerations suggest even a negative relationship between metabolic rate and oxidative stress. We investigated the relationship between standard metabolic rate (SMR), antioxidants, and damage in blood plasma and erythrocytes for 35 grass snakes (Natrix natrix). Reactive oxygen metabolites (dROMs) and nonenzymatic antioxidants were assessed in plasma, while two measures of DNA damage and the capacity to neutralize H2O2 were measured in erythrocytes. Plasma antioxidants showed no correlation to SMR, and the level of dROMs was positively related to SMR. A negative relationship between antioxidant capacity and SMR was found in erythrocytes, but no association of SMR with either measure of DNA damage was detected. No increase in DNA damage, despite lower antioxidant capacity at high SMR, indicates an upregulation in other defense mechanisms (e.g., damage repair and/or removal). Indeed, we observed a higher frequency of immature red blood cells in individuals with higher SMR, which indicates that highly metabolic individuals had increased erythrocyte turnover, a mechanism of damage removal. Such DNA protection through upregulated cellular turnover might explain the negligible senescence observed in some ectotherm taxa.


Assuntos
Metabolismo Basal/fisiologia , Colubridae/fisiologia , Dano ao DNA , Envelhecimento , Animais , Antioxidantes/análise , Colubridae/metabolismo , Eritrócitos/citologia , Eritrócitos/metabolismo , Feminino , Peróxido de Hidrogênio/metabolismo , Masculino , Estresse Oxidativo/fisiologia , Plasma/metabolismo , Espécies Reativas de Oxigênio/sangue
12.
Nurs Res ; 69(3): 244-248, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31917737

RESUMO

BACKGROUND: A known relationship exists between oxidative stress and preterm birth (PTB). However, few studies have measured oxidative stress prospectively in early or midpregnancy, and no studies have used electron paramagnetic resonance (EPR) spectroscopy prospectively to predict PTB. OBJECTIVE: The purpose of this study was to identify predictive relationships between antioxidants and reactive oxygen species (ROS), specifically, superoxide (O2), peroxynitrite (OONO), and hydroxyl radical (OH), using EPR spectroscopy, measured between 12 and 20 weeks of gestation and compare with the incidence of PTB. METHODS: Blood was obtained from pregnant women (n = 140) recruited from a tertiary perinatal center. Whole blood was analyzed directly for ROS, O2, OONO, and OH using EPR spectroscopy. Red blood cell lysate was used to measure antioxidants. PTB was defined as parturition at <37 weeks of gestation. RESULTS: No differences were found between ROS, O2, OONO, or OH with the incidence of PTB. Catalase activity, glutathione, and reduced/oxidized glutathione ratio were significantly lower with PTB. Logistic regression suggests decreased catalase activity in pregnant women is associated with increased odds of delivering prematurely. DISCUSSION: We prospectively compared antioxidants and specific ROS using EPR spectroscopy in pregnant women between 12 and 20 weeks of gestation with the incidence of PTB. Results are minimal but do suggest that antioxidants-specifically decreased catalase activity-in early pregnancy may be associated with PTB; however, these findings should be cautiously interpreted and may not have clinical significance.


Assuntos
Idade Gestacional , Estresse Oxidativo , Nascimento Prematuro/epidemiologia , Antioxidantes/análise , Espectroscopia de Ressonância de Spin Eletrônica , Feminino , Humanos , Gravidez , Estudos Prospectivos , Espécies Reativas de Oxigênio/sangue
13.
J Anim Physiol Anim Nutr (Berl) ; 104(1): 196-203, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31701584

RESUMO

The present experiment was conducted to evaluate the effect of dietary supplementation with some feed additives (potassium sorbate; Sor, hydrated sodium calcium almuniosilicate; Hsc and L-methionine; L-M) against aflatoxin B1 (AF) toxicity in rabbits. A total of 72 growing rabbits (5-week-old) were distributed into six equal groups (4 replicates with 3 rabbits each). The experimental groups are as follows: control group, AF group (supplemented with AF 0.3 mg/kg diet), AF + Sor group (AF 0.3 mg/kg diet + Sor 2 g/kg diet), AF + Hsc group (AF 0.3 mg/kg diet + Hsc 5 g/kg diet), AF + L-M group (AF 0.3 mg/kg diet + L-M 8 g/kg diet) and AF + Mix group (AF 0.3 mg/kg diet + 2 Sor + 5 Hsc + 8 L-M g/kg diet). Live body weight and weight gain at 13 weeks of age were significantly reduced by AF. Feed intake at 13 weeks of age was decreased in AF, AF + Hsc and AF + Mix compared to the control. AF, AF + Hsc and AF + Mix showed the lowest total antioxidant capacity compared to the control. The highest level of reactive oxygen species and 8-Hydroxy-2-desoxyguanosine was observed in AF group. Using of other supplements with AF increased immunoglobulinM than AF alone. In conclusion, dietary supplementation of Sor, L-M, Hsc or their mixture was effective in reducing the adverse effects of AF on performance, antioxidant and immune status of rabbits with more better improvement obtained by Sor or L-M separately.


Assuntos
Aflatoxina B1/toxicidade , Silicatos de Alumínio/farmacologia , Metionina/farmacologia , Coelhos/crescimento & desenvolvimento , Ácido Sórbico/farmacologia , 8-Hidroxi-2'-Desoxiguanosina/sangue , Silicatos de Alumínio/administração & dosagem , Ração Animal/análise , Fenômenos Fisiológicos da Nutrição Animal , Animais , Dieta/veterinária , Suplementos Nutricionais , Contaminação de Alimentos , Imunoglobulina M/sangue , Malondialdeído/sangue , Metionina/administração & dosagem , Coelhos/sangue , Espécies Reativas de Oxigênio/sangue , Ácido Sórbico/administração & dosagem
14.
Leuk Res ; 88: 106268, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-31760269

RESUMO

The developing fetus is exposed to chemicals, which are metabolized to electrophiles that form adducts with nucleophilic Cys34 of human serum albumin (HSA). By measuring these adducts in neonatal blood spots (NBS), we obtain information regarding fetal exposures during the last month of gestation. To discover potential risk factors for childhood leukemia resulting from in utero exposures, we used untargeted adductomics to measure HSA-Cys34 adducts in 782 archived NBS, collected from incident cases of childhood acute lymphoblastic leukemia (ALL) or acute myeloid leukemia (AML) and matched population-based controls. Among a total of 28 Cys34 modifications that were measured, we found no differences in adduct abundances between childhood leukemia cases and controls overall. However, cases of T-cell ALL had higher abundances of adducts of reactive carbonyl species and a Cys34 disulfide of homocysteine was present at lower levels in AML cases. These results suggest that oxidative stress and lipid peroxidation may be etiologic factors of T-cell ALL, and alterations in one-carbon metabolism and epigenetic changes may be predictors of AML. Future replication of the results with larger sample sizes is necessary.


Assuntos
Teste em Amostras de Sangue Seco , Leucemia/diagnóstico , Triagem Neonatal/métodos , Proteômica/métodos , Espécies Reativas de Oxigênio/análise , Albumina Sérica Humana/análise , Adolescente , Idade de Início , Estudos de Casos e Controles , Criança , Pré-Escolar , Cromatografia Líquida de Alta Pressão , Teste em Amostras de Sangue Seco/métodos , Feminino , Humanos , Lactente , Recém-Nascido , Leucemia/sangue , Leucemia/epidemiologia , Peroxidação de Lipídeos , Masculino , Estresse Oxidativo/fisiologia , Processamento de Proteína Pós-Traducional , Espécies Reativas de Oxigênio/sangue , Albumina Sérica Humana/metabolismo
15.
Exp Clin Endocrinol Diabetes ; 128(4): 216-223, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30508848

RESUMO

AIMS: Prevalence of gestational diabetes mellitus (GDM) may vary across a country like India. Risk factors and disease-pathogenesis were also not fully elucidated. This study aimed to examine prevalence of GDM among pregnant women visiting antenatal clinic of a tertiary-care hospital of Kolkata, India; possible mechanism of disease pathogenesis and potency of associated parameters as disease biomarkers were also explored. METHODS: 735 pregnant women were screened for GDM according to DIPSI (Diabetes in Pregnancy Study Group India) guideline and risk-factors were analyzed. Case-control study was conducted with 114 GDM and 114 matched non-GDM control. Blood sample was collected before glucose load for complete blood count, measurement of reactive oxygen species (ROS) and assessment of DNA damage. RESULTS: Prevalence of GDM was found to be 17.2%(127/735). Maternal age, diabetic family history and acanthosis nigricans seemed to be important risk factors. Total ROS, lymphocyte DNA damage (measured by comet-assay) and some inflammatory hematological parameters were significantly higher in GDM compared to control. ROS, comet-tail DNA%, WBC, neutrophil-lymphocyte ratio (NLR) and mean platelet volume (MPV) were established as independent determinants of disease condition after adjustment for pre-gestational body mass index. In receiver operating characteristic analysis, ROS>155.7 arbitrary fluorescent unit, NLR>2.12 and MPV>11.05 fL showed 82.5 & 98.2%, 71.9 & 84.2% and 71.9 & 82.5% sensitivity & specificity respectively in disease prediction. CONCLUSIONS: Prevalence of GDM seemed to be high in Kolkata on Indian scenario. Oxidative-stress, related DNA-damage and inflammation seemed to have important contribution in pathogenesis of GDM independent of obesity. ROS, NLR and MPV with respective cut-off scores might be used as diagnostic and prognostic biomarkers for better management of the disease.


Assuntos
Diabetes Gestacional/sangue , Diabetes Gestacional/epidemiologia , Diabetes Gestacional/etiologia , Inflamação/sangue , Espécies Reativas de Oxigênio/sangue , Acantose Nigricans/epidemiologia , Adulto , Biomarcadores/sangue , Estudos de Casos e Controles , Estudos Transversais , Feminino , Predisposição Genética para Doença/epidemiologia , Humanos , Índia/epidemiologia , Inflamação/epidemiologia , Gravidez , Prevalência , Fatores de Risco , Centros de Atenção Terciária , Adulto Jovem
16.
BMC Pharmacol Toxicol ; 20(1): 74, 2019 12 02.
Artigo em Inglês | MEDLINE | ID: mdl-31791417

RESUMO

BACKGROUND: Anti-neoplastic agents are widely used in the treatment of cancer and some non-neoplastic diseases. These drugs have been proved to be carcinogens, teratogens, and mutagens. Concern exists regarding the possible dangers of the staff handling anti-cancer drugs. The long-term exposure of nurses to anti-neoplastic drugs is still a controversial issue. The purpose of this study was to monitor cellular toxicity parameters and gene expression in nurses who work in chemotherapy wards and compare them to nurses who work in other wards. METHODS: To analyze the apoptosis-related genes overexpression and cytotoxicity effects, peripheral blood lymphocytes obtained from oncology nurses and the control group. THE RESULTS: Significant alterations in four analyzed apoptosis-related genes were observed in oncology nurses. In most individual samples being excavated, Bcl-2 overexpression is superior to that of Bax. Prominent P53 and Hif-1α up-regulation were observed in oncology nurses. Moreover, all cytotoxicity parameters (cell viability, ROS formation, MMP collapse, Lysosomal membrane damage, Lipid peroxidation, Caspase 3 activity and Apoptosis phenotype) in exposed oncology nurses were significantly (p < 0.001) higher than those of unexposed control nurses. Up-regulation of three analyzed apoptosis-related genes were observed in nurses occupationally exposed to anti-cancer drugs. CONCLUSION: Our data show that oxidative stress and mitochondrial toxicity induced by anti-neoplastic drugs lead to overexpression of apoptosis-related genes in oncology nurses.


Assuntos
Antineoplásicos/efeitos adversos , Proteínas Reguladoras de Apoptose/genética , Expressão Gênica/efeitos dos fármacos , Linfócitos/efeitos dos fármacos , Enfermeiras e Enfermeiros , Exposição Ocupacional/efeitos adversos , Adulto , Antineoplásicos/administração & dosagem , Antineoplásicos/sangue , Apoptose/efeitos dos fármacos , Apoptose/genética , Estudos de Casos e Controles , Caspase 3/sangue , Feminino , Humanos , Peroxidação de Lipídeos/efeitos dos fármacos , Linfócitos/enzimologia , Linfócitos/fisiologia , Masculino , Potencial da Membrana Mitocondrial/efeitos dos fármacos , Exposição Ocupacional/análise , Espécies Reativas de Oxigênio/sangue
17.
Nutr J ; 18(1): 89, 2019 12 26.
Artigo em Inglês | MEDLINE | ID: mdl-31878925

RESUMO

BACKGROUND: The aim of this study was to evaluate markers of inflammation, oxidative stress and endothelial function in a disease-related malnutrition (DRM) outpatient population. METHODS: For this cross-sectional study, a total of 83 subjects were included and clustered in 3 groups: 34 with normonutrition (NN), 21 with DRM without inflammation (DRM-I) and 28 with DRM and inflammation (DRM + I). Nutritional diagnosis was conducted for all subjects according to ASPEN. Biochemical parameters, proinflammatory cytokines, reactive oxygen species production, glutathione, mitochondrial membrane potential, oxygen consumption, adhesion molecules and leukocyte-endothelium interactions were evaluated. RESULTS: DRM + I patients showed lower albumin, prealbumin, transferrin, and retinol-binding protein levels with respect to the NN group (p < 0.05), differences that were less noticeable in the DRM-I group. DRM + I was associated with a significant increase in hsCRP and IL6 vs the NN and DRM-I groups, and TNFα was increased in both DRM vs NN. DRM was characterised by increased oxidative stress, which was marked by a significant increase in ROS levels and a decrease in mitochondrial membrane potential in the DRM + I group. An evident reduction in mitochondrial oxygen consumption and glutathione concentration was observed in both DRM groups, and was accompanied by increased leukocyte adhesion and adhesion molecules and decreased rolling velocity in the DRM + I group. Furthermore, percentage of weight loss was negatively correlated with albumin, prealbumin, transferrin, O2 consumption, glutathione and leukocyte rolling velocity, and positively correlated with hsCRP, IL6, TNFα, ROS, leukocyte adhesion, and VCAM-1. CONCLUSIONS: Our results show that DRM is associated with oxidative stress and an inflammatory state, with a deterioration of endothelial dysfunction in the DRM + I population.


Assuntos
Leucócitos/fisiologia , Desnutrição/sangue , Desnutrição/complicações , Mitocôndrias/fisiologia , Estresse Oxidativo , Idoso , Adesão Celular , Estudos Transversais , Citocinas/sangue , Feminino , Glutationa/sangue , Humanos , Inflamação/sangue , Inflamação/fisiopatologia , Masculino , Potencial da Membrana Mitocondrial , Pessoa de Meia-Idade , Oxigênio/sangue , Espécies Reativas de Oxigênio/sangue , Espanha
18.
BMC Musculoskelet Disord ; 20(1): 589, 2019 Dec 07.
Artigo em Inglês | MEDLINE | ID: mdl-31810443

RESUMO

BACKGROUND: Recent reports indicate that oxidative stress induced by reactive oxygen species is associated with the pathobiology of neurodegenerative disorders that involve neuronal cell apoptosis. Here we conducted a cross-sectional study to evaluate serum levels of oxidative stress in cervical compression myelopathy. METHODS: Thirty-six serum samples were collected preoperatively from patients treated for acutely worsening compression myelopathy (AM) and chronic compression myelopathy (CM). Serum levels of oxidative stress markers were evaluated by measuring derivatives of reactive oxygen metabolites (ROM), which reflect concentrations of hydroperoxides. ROM in healthy individuals range from 250 to 300 (U. CARR), whereas ROM >340-400 and > 400 define moderate and severe levels of oxidative stress, respectively. Difference of ROM by the cause of disorders whether cervical spondylotic myelopathy (CSM) or cervical ossification of longitudinal ligament (OPLL), correlations between ROM and patient age, body mass index (BMI), history of smoking, existence of diabetes were examined. Neurological evaluations according to Japanese Orthopaedic Association (JOA) scores were performed and correlated with ROM. RESULTS: ROM increased to 349.5 ± 54.8, representing a moderate oxidative stress, in CM samples. ROM increased to 409.2 ± 77.9 in AM samples, reflecting severe oxidative stress which were significantly higher than for CM samples (p < 0.05). There was no significant difference by the cause of disorders (CSM or OPLL). ROM were significantly increased in AM serum samples from female patients versus AM male and CM patients (p < 0.05). There were no correlations between ROM and age, BMI, history of smoking, and existence of diabetes. A negative correlation between ROM and recovery rate of JOA score (R2 = 0.454, p = 0.047) was observed in the AM group. CONCLUSIONS: Although moderate oxidative stress was present in patients with CM, levels of oxidative stress increased in severity in patients with AM. These results suggest that postsurgical neurological recovery is influenced by severe oxidative stress in AM.


Assuntos
Vértebras Cervicais/fisiopatologia , Ossificação do Ligamento Longitudinal Posterior/cirurgia , Espécies Reativas de Oxigênio/sangue , Compressão da Medula Espinal/cirurgia , Adulto , Idoso , Idoso de 80 Anos ou mais , Biomarcadores/sangue , Estudos Transversais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Ossificação do Ligamento Longitudinal Posterior/sangue , Ossificação do Ligamento Longitudinal Posterior/diagnóstico , Ossificação do Ligamento Longitudinal Posterior/patologia , Estresse Oxidativo/fisiologia , Período Pré-Operatório , Espécies Reativas de Oxigênio/metabolismo , Recuperação de Função Fisiológica/fisiologia , Índice de Gravidade de Doença , Compressão da Medula Espinal/sangue , Compressão da Medula Espinal/diagnóstico , Compressão da Medula Espinal/patologia , Resultado do Tratamento
19.
Int J Mol Sci ; 20(21)2019 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-31690051

RESUMO

Oxygen (O2) is commonly used in clinical practice to prevent or treat hypoxia, but if used in excess (hyperoxia), it may act as toxic. O2 toxicity arises from the enhanced formation of Reactive Oxygen Species (ROS) that exceed the antioxidant defenses and generate oxidative stress. In this study, we aimed at assessing whether an elevated fraction of inspired oxygen (FiO2) during and after general anesthesia may contribute to the unbalancing of the pro-oxidant/antioxidant equilibrium. We measured five oxidative stress biomarkers in blood samples from patients undergoing elective abdominal surgery, randomly assigned to FiO2 = 0.40 vs. 0.80: hydroperoxides, antioxidants, nitrates and nitrites (NOx), malondialdehyde (MDA), and glutathionyl hemoglobin (HbSSG). The MDA concentration was significantly higher 24 h after surgery, and the body antioxidant defense lower, in the FiO2 = 0.80 group with respect to both the FiO2 = 0.40 group and the baseline values (p ≤ 0.05, Student's t-test). HbSSG in red blood cells was also higher in the FiO2 = 0.80 group at the end of the surgery. NOx was higher in the FiO2 = 0.80 group than the FiO2 = 0.40 group at t = 2 h after surgery. MDA, the main end product of the peroxidation of polyunsaturated fatty acids directly influenced by FiO2, may represent the best marker to assess the pro-oxidant/antioxidant equilibrium after surgery.


Assuntos
Anestesia/efeitos adversos , Glutationa/sangue , Hiperóxia/diagnóstico , Malondialdeído/sangue , Espécies Reativas de Oxigênio/sangue , Idoso , Biomarcadores/sangue , Feminino , Hemoglobinas , Humanos , Hiperóxia/sangue , Hiperóxia/etiologia , Masculino , Monitorização Intraoperatória/métodos , Estresse Oxidativo
20.
Physiol Rep ; 7(21): e14277, 2019 11.
Artigo em Inglês | MEDLINE | ID: mdl-31691539

RESUMO

The duathlon is an endurance multisport event that consists of sequential running, cycling, and further running. Imbalance in the redox homeostasis is associated with fatigued status and underperformance in various sports; however, there are no corresponding reports regarding the duathlon. The purpose of this study was to examine the changes in the blood redox balance during a simulated duathlon race and to determine the relationship between performance and the redox balance. Eight male triathletes participated in a simulated race, consisting of a 5-km run, 30 km cycling, and a further 5-km run, with 5 min rest between two parts to collect the blood samples. The serum levels of reactive oxygen metabolites (d-ROMs) and biological antioxidant potential (BAP) were measured and BAP/d-ROMs ratio and oxidative stress index (OSI) were calculated. The d-ROMs levels after the first Run were significantly increased compared with the levels observed before the race. Moreover, BAP levels increased significantly over the race. The BAP/d-ROMs ratio also gradually increased through the race, while the OSI was gradually decreased. In addition, a significant relationship was observed only between d-ROMs levels after the first Run and the first Run performance. These results suggest that the redox balance shifts toward reduction (antioxidation) during the duathlon race and increased oxidant potential levels are negatively correlated with performance in the early stages of the race.


Assuntos
Desempenho Atlético/fisiologia , Sangue/metabolismo , Exercício Físico/fisiologia , Adulto , Antioxidantes , Biomarcadores/sangue , Humanos , Masculino , Oxirredução , Estresse Oxidativo , Oxigênio/sangue , Espécies Reativas de Oxigênio/sangue , Adulto Jovem
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