Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 91.361
Filtrar
1.
Se Pu ; 37(8): 788-797, 2019 Aug 08.
Artigo em Chinês | MEDLINE | ID: mdl-31642248

RESUMO

The structures of proteins are directly related to their biological functions. The regulation of protein functions mainly depends on the dynamic regulation of their conformations and interactions. The study of protein structures and functions plays an essential role in the field of life science, which is also an important direction of proteomics development. The emergence of proteomics has led to major technological advances in mass spectrometry (MS). These advancements have not only benefitted MS-based high-throughput proteomics but have also increased the impact of MS in the fields of structural and molecular biology. This review briefly describes the principles, progresses and applications of the MS-based structural proteomics methods in recent years, including the native MS analysis of active proteins, limited proteolysis, chemical cross-linking, hydrogen-deuterium exchange, covalent labeling, and thermal proteome profiling. Finally, future development of the structural proteomics was summarized and prospected.


Assuntos
Proteínas/química , Proteômica , Espectrometria de Massas , Proteólise
2.
Se Pu ; 37(8): 863-871, 2019 Aug 08.
Artigo em Chinês | MEDLINE | ID: mdl-31642257

RESUMO

Exosomes are tiny vesicles secreted by cells, can be important mediators of cell-to-cell communication, and play unique roles in disease diagnosis and treatment. Osteoporosis is a metabolic bone disease with high incidence in the elderly, characterized by low bone mineral density and deterioration of bone microstructure. Highly specific diagnostic methods capable of identifying early-stage osteoporosis are urgently needed. In this study, serum exosomes were comprehensively enriched and characterized. In total, 179 exosomal proteins were identified using liquid chromatography-mass spectrometry, most of which are involved in important biological processes such as defense and immune responses. Through label free quantification of serum exosomes, 188, 224, and 185 proteins were identified in the normal, osteopenia, and osteoporosis groups, respectively. Quantitative results also showed that 17 proteins were significantly (p <0.05) dysregulated in the osteoporosis and osteopenia groups, including Integrin ß 3, Integrin α 2 ß 1, Talin 1, and Gelsolin. This study provides potential molecular markers for osteoporosis studs and will contributes to the elucidating the pathogenesis of osteoporosis.


Assuntos
Exossomos , Osteoporose/sangue , Proteômica , Cromatografia Líquida , Humanos , Espectrometria de Massas
3.
Se Pu ; 37(8): 897-903, 2019 Aug 08.
Artigo em Chinês | MEDLINE | ID: mdl-31642261

RESUMO

The global increase in the prevalence of gestational diabetes mellitus(GDM)in recent years has prompted the study of the effect of GDM on the metabolism between mother and fetus. In this study, the metabolomic investigation of the umbilical cord blood of mothers presenting GDM was performed using liquid chromatography-mass spectrometry (LC-MS), orthogonal projections to latent structures discriminant analysis (OPLS-DA), and network analysis to assess GDM-related metabolic biomarkers. The results showed that arachidonic acid (AA) played an important role in the key metabolic network while further pathway analysis suggested that GDM induced unsaturated fatty acid metabolic disorder. This study provides the underlying metabolic mechanism of GDM-induced metabolic abnormalities between mother and fetus.


Assuntos
Diabetes Gestacional/metabolismo , Sangue Fetal/metabolismo , Metabolômica , Ácido Araquidônico , Biomarcadores/sangue , Cromatografia Líquida , Feminino , Humanos , Espectrometria de Massas , Gravidez
4.
Se Pu ; 37(8): 904-910, 2019 Aug 08.
Artigo em Chinês | MEDLINE | ID: mdl-31642262

RESUMO

One of the most abundant biological volatile organic compounds (BVOCs) in the atmosphere, monoterpene, is characterized by its short lifetime, low concentration, fast temporal and spatial variations, and wide variety of isomers. In this study, a multi-capillary column (MCC) was combined with high-pressure photoionization time-of-flight mass spectrometry (HPPI-TOF MS) and employed to develop an MCC-HPPI-TOF MS combination instrument as an online two-dimensional gas chromatography-mass spectrometry (GC-MS) method for the rapid qualitative and quantitative analysis of monoterpene isomers. As a result, six monoterpene isomers, α -pinene, ß -pinene, α -terpinene, γ -terpinene, 3-carene, and limonene, were successfully isolated in 180 s with limits of detection (LODs) as low as 6 µg/m3 without sample pre-enrichment. This method was successfully applied to the rapid online analysis of monoterpenes released from the branches and leaves of Cedrus atlantica and Sabina chinensis, which shows the capability and potential application of the method for the online detection of complex sample mixtures in environmental monitoring, process analysis, and other fields.


Assuntos
Monoterpenos/análise , Compostos Orgânicos Voláteis/análise , Cromatografia Gasosa-Espectrometria de Massas , Espectrometria de Massas
5.
Se Pu ; 37(8): 911-917, 2019 Aug 08.
Artigo em Chinês | MEDLINE | ID: mdl-31642263

RESUMO

A liquid chromatography method was established for the determination of zearalanone (ZAN) raw material. The qualitative analysis of ZAN and its trace impurities was performed by ultra performance liquid chromatography-diode array detector (UPLC-DAD) and ultra performance liquid chromatography-high resolution mass spectrometry (UPLC-HRMS), and the response factors of each impurity were calculated. The three main organic impurities in the ZAN raw material were identified as ß -zearalanol, α -zearalanol and a dehydration product of zearalanol with relative response factors of 0.5352, 0.8594 and 0.6973, respectively. The main component of the ZAN raw material was determined by the calibration factor normalization method. The purity of zearalanone was determined to be 99.6% with a standard deviation of 0.01%. This method can provide a technical support for the development of ZAN standard materials.


Assuntos
Zearalenona/análise , Cromatografia Líquida de Alta Pressão , Espectrometria de Massas , Análise de Componente Principal
6.
Se Pu ; 37(9): 939-945, 2019 Sep 08.
Artigo em Chinês | MEDLINE | ID: mdl-31642297

RESUMO

To investigate the immunomodulatory mechanism of low relative molecular mass seleno-aminopolysaccharide, a metabolomics method based on liquid chromatography-time-of-flight mass spectrometry (LC-TOF-MS) was used to analyze the endogenous metabolites changes in the liver of Acanthopagrus schlegelii. The potential biomarkers were screened using non-targeted mass spectrometry with the XCMSplus software, and the related metabolic pathways were analyzed using MetaboAnalyst3.0 website. The results showed that the liver metabolites in the low relative molecular mass seleno-aminopolysaccharide-fed group were significantly different from those in the blank group. Also, 32 biomarkers were identified. Additionally, low relative molecular mass seleno-aminopolysaccharide could enhance the immune function of Acathopagrus schlegelii via amino acid, nucleotide, and nitrogen metabolism, aminoacyl-transfer ribonucleic acid (tRNA) biosynthesis, and other metabolic pathways. This study therefore provides a scientific basis for elucidating the immunoenhancement mechanism of low relative molecular mass seleno-aminopolysaccharide.


Assuntos
Fígado/efeitos dos fármacos , Metabolômica , Polissacarídeos/farmacologia , Dourada , Compostos de Selênio/farmacologia , Animais , Biomarcadores , Cromatografia Líquida , Espectrometria de Massas , Redes e Vias Metabólicas
7.
Se Pu ; 37(9): 955-962, 2019 Sep 08.
Artigo em Chinês | MEDLINE | ID: mdl-31642299

RESUMO

An improved method to screen 52 pesticide residues in flavored tea using QuEChERS coupled with liquid chromatography-quadrupole-time-of-flight mass spectrometry (LC-Q-TOF-MS) has been investigated. The flavored tea was extracted with acetonitrile and purified using by primary secondary amine (PSA), graphitized carbon black (GCB) and C18 sorbent. The resultant residues were then analyzed using LC-Q-TOF-MS. The recoveries of all the pesticides in flavored tea at the four spiked levels of 10, 20, 50 and 100 µg/kg were 70%-120% and the relative standard deviations (RSDs, n=3) were less than 20%. The calibration curves of the 52 pesticide residues had good linear relationships, and the correlation coefficients were more than 0. 99. The screening detection limits (SDLs) and the limits of quantitation (LOQs) for the 52 pesticides were in the range of 0.001-0.01 mg/kg and 0.002-0.02 mg/kg, respectively, which were lower than maximum residue limits standard permitted by the European Union (EU). This method is simple, rapid, reliable, and can meet the requirements for the simultaneous determination of 52 pesticide residues in flavored tea.


Assuntos
Contaminação de Alimentos/análise , Resíduos de Praguicidas/análise , Chá/química , Cromatografia Líquida , Aromatizantes , Espectrometria de Massas
8.
Se Pu ; 37(9): 1011-1018, 2019 Sep 08.
Artigo em Chinês | MEDLINE | ID: mdl-31642307

RESUMO

In this study, ultra performance liquid chromatography-quadrupole electrostatic field orbitrap mass spectrometry was used to establish a high-throughput screening method for 20 ß -blockers and their metabolites in foods of animal origin. The sensitivity and applicability of the established method were improved by optimizing the instrument and pretreatment conditions. The samples were purified by high speed centrifugation at low temperature, and the compounds were separated by a C8 column. Qualitative and quantitative analyses of the compounds were performed in full MS/dd-MS2 (data-dependent MS2) mode. Twenty compounds showed a good linear relationship in the range 0.1-10 µg/L, with correlation coefficients (r2) greater than 0.99. The limits of detection (LODs) ranged from 1 to 5 µg/kg, while the limits of quantification (LOQs) ranged from 2 to 10 µg/kg. The average recoveries were 60.37%-100.84%, with relative standard deviations less than 10%. The method is operationally simple and has good reproducibility and high accuracy, which are essential for ß -blockers and metabolite residue screening in foods of animal-origin.


Assuntos
Antagonistas Adrenérgicos beta/análise , Resíduos de Drogas/análise , Contaminação de Alimentos/análise , Animais , Cromatografia Líquida de Alta Pressão , Limite de Detecção , Espectrometria de Massas , Carne/análise , Reprodutibilidade dos Testes
9.
Zhongguo Zhong Yao Za Zhi ; 44(14): 3078-3086, 2019 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-31602856

RESUMO

The element speciation analysis for heavy metals in herbal medicines is still in the beginning stage. In this study,the total amount of arsenic( As) in 103 batches of 17 commonly used Chinese medicines( including 16 plant medicines and 1 medicinal fungus) was detected by inductively coupled plasma mass spectrometry( ICP-MS). Furthermore,based on HPLC-ICP-MS,the simultaneous detection methods of six As speciation kinds in traditional Chinese medicines were established. An AS7 anion exchange column was selected and the As forms in 17 traditional Chinese medicines was systematically analyzed. The results showed that the method of pretreatment of medicinal materials by microwave digestion and the detection of total amount of As by ICP-MS was stable and reliable. As for the speciation analysis of As,the high-speed ultrasonic extraction method was adopted,and it showed that the linear relationship of the six As speciation was satisfied with the correlation coefficient R2>0. 999 9. The LOQ of six kinds of As speciation were 0. 20,0. 10,0. 15,0. 10,0. 25,0. 10 µg·L~(-1) for arsenic betaine( As B),arsenious acid [As( Ⅲ) ],dimethyl arsenic( DMA),arsenic choline( As C),monomethyl arsenic( MMA),arsenic acid[As( Ⅴ) ],respectively. The recoveries were between 84. 24% and 121. 5%,and the relative standard deviations were 2. 7% to 11%. Among the 103 batches of medicinal materials,only one batch of sample As exceeded the Chinese Pharmacopoeia limit standard; As( Ⅲ) and As( Ⅴ) had high detection rate in 103 batches of Chinese herbal medicines,within which As( Ⅴ) was the main detected form,and inorganic As accounted for the ratio reached 80. 90%-98. 73%; some samples detected DMA,MMA and As B,As C was not detected in any batch. This study established an analytical method suitable for the speciation of As in Chinese herbal medicines,and provided basic data for As residual residue in Chinese herbal medicines,which can provide important reference for the risk assessment and quality standards.


Assuntos
Arsênico/análise , Medicamentos de Ervas Chinesas/análise , Cromatografia Líquida de Alta Pressão , Medicamentos de Ervas Chinesas/normas , Espectrometria de Massas
10.
Zhongguo Zhong Yao Za Zhi ; 44(16): 3562-3568, 2019 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-31602923

RESUMO

The mass spectrometry-based metabolomics method was used to systematically investigate the formation of celastrol metabolites,and the effect of celastrol on endogenous metabolites. The mice plasma,urine and feces samples were collected after oral administration of celastrol. Ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry( UPLC-QTOF-MS) was applied to analyze the exogenous metabolites of celastrol and its altered endogenous metabolites. Mass defect filtering was adopted to screen for the exogenous metabolites of celastrol. Multivariate statistical analysis was used to identify the endogenous metabolites affected by celastrol. Celastrol and its eight metabolites were detected in urine and feces of mice,and 5 metabolites of them were reported for the first time. The hydroxylated metabolites were observed in the metabolism of both human liver microsomes and mouse liver microsomes. Further recombinant enzyme experiments revealed CYP3 A4 was the major metabolic enzyme involved in the formation of hydroxylated metabolites. Urinary metabolomics revealed that celastrol can affect the excretion of intestinal bacteria-related endogenous metabolites,including hippuric acid,phenylacetylglycine,5-hydroxyindoleacetic acid,urocanic acid,cinnamoylglycine,phenylproplonylglycine and xanthurenic acid. These results are helpful to elucidate the metabolism and disposition of celastrol in vivo,and its mechanism of action.


Assuntos
Metabolômica , Triterpenos/farmacocinética , Animais , Cromatografia Líquida de Alta Pressão , Humanos , Espectrometria de Massas , Camundongos , Microssomos Hepáticos/metabolismo , Triterpenos/metabolismo
11.
Zhongguo Zhong Yao Za Zhi ; 44(17): 3724-3731, 2019 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-31602945

RESUMO

Testing and analysis of chemical markers is currently the prevailing approach for quality assessment of traditional Chinese medicines. However,several important issues remain to be addressed,including the trade-off between accuracy and coverage. In this study,in order to give full play to the advantages of their respective methods and provide technical support for more comprehensively and rapidly evaluate the quality of Danshen Injection products,a fingerprint method was coupled with quantitative analysis of multicomponents by single marker( QAMS),with Danshen Injection as the carrier. Ultra performance liquid chromatography( UPLC) was used to establish the quantitative fingerprint. The UPLC fingerprints contained 13 common peaks,11 of which were identified by using ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry( UPLC-QTOF-MS). Furthermore,with sodium danshensu as the internal reference substance,relative correction factors( RCFs) of protocatechuic aldehyde,caffeic acid,rosmarinic acid,lithospermic acid,and salvianolic acid B were calculated through slope analysis method,and the QAMS method was adopted to determine the contents of these 6 components. The UPLC fingerprint was employed to assess the consistency of 12 batches of Danshen Injection,which showed good batch-to-batch consistency with similarity higher than 0. 99. In the comparison of contents of the six constituents obtained by QAMS and external standard method( ESM),RSD was all less than 4. 3%,indicating the good accuracy of the QAMS method. The QAMS method developed in this study combined with UPLC fingerprint can comprehensively reflect the internal quality of Danshen Injection when only the reference substance sodium danshensu is consumed,with greatly reduced detection cost and time. It provides a technical basis for further improving the quality standard of Danshen Injection.


Assuntos
Medicamentos de Ervas Chinesas/normas , Salvia miltiorrhiza/química , Cromatografia Líquida de Alta Pressão , Medicamentos de Ervas Chinesas/análise , Injeções , Espectrometria de Massas , Controle de Qualidade
12.
Zhongguo Zhong Yao Za Zhi ; 44(17): 3798-3805, 2019 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-31602956

RESUMO

Based on metabolomics,the metabolites of larvae zebrafish with overdose of Panax notoginseng saponins( PNS) were compared with those in normal group of larvae zebrafish to investigate the possible toxicity mechanism of overdose PNS in larvae zebrafish. An experimental animal model of long-term toxicity induced by PNS overdose was established by administering 1-6 dpf at low,medium and high doses of PNS,respectively. The ultra-performance liquid chromatography-quadrupole-time of flight mass spectrometry( UPLC-Q-TOF-MS) technique was combined with principal component analysis( PCA) and orthogonal partial least squares discriminant analysis( OPLS-DA) to screen and identify biomarkers associated with toxicity,and then the MetaboAnalyst database was used to analyze metabolism-related pathways. The results showed that the metabolites of each group could be distinguished distinctly,and they deviated more from the normal group in a time and dose dependent manner. Twenty-nine potential biomarkers related to toxicity( VIP>1,P<0. 05) were identified preliminarily,mainly involving six metabolic pathways. From the metabonomics point of view,the toxicity mechanism of overdose PNS may be related to the disorders of lipid metabolism,amino acid metabolism and energy metabolism.


Assuntos
Metabolômica , Panax notoginseng/toxicidade , Saponinas/toxicidade , Aminoácidos/metabolismo , Animais , Cromatografia Líquida de Alta Pressão , Metabolismo Energético , Larva/efeitos dos fármacos , Metabolismo dos Lipídeos , Espectrometria de Massas , Testes de Toxicidade Aguda , Peixe-Zebra
13.
Se Pu ; 37(11): 1142-1156, 2019 Nov 08.
Artigo em Chinês | MEDLINE | ID: mdl-31642266

RESUMO

An ultra-performance liquid chromatography-triple quadrupole/linear ion trap mass spectrometry (UPLC-Qtrap MS) method was developed for the determination of 84 toxic plant constiuents in plasma and urine. Plasma was precipitated by acetonitrile to remove proteins and then passed through a Prime HLB SPE column to remove phospholipids, while urine was diluted with methanol. Chromatographic separation of the analytes was achieved on an Acquity BEH C18 column (100 mm×2.1 mm, 1.7 µm) by gradient elution using the mobile phase of 0.1% (v/v) formic acid and 2 mmol/L ammonium formate both in 97% (v/v) acetonitrile aqueous solution and water. Electrospray ionization mass spectrometry was carried out in the positive ion mode with multiple reaction monitoring-information dependent acquisition-enhanced product ion scan mode (MRM-IDA-EPI). The 84 analytes were quantified by the matrix working standard curve internal standard method, and a good linear relationship was observed, with correlation coefficients of ≥ 0.9911. The limits of detection (LODs) in plasma and urine were 0.01-1 µg/L and 0.03-2 µg/L, respectively. The intra- and inter-day precisions of these analytes were 0.7%-18.4% and 1.1%-18.5%, and the accuracy of all analytes ranged from 70.6% to 124.5%. This method is simple, sensitive, and accurate for the measurement of these analytes in plasma and urine for both clinical and forensic applications.


Assuntos
Compostos Fitoquímicos/sangue , Compostos Fitoquímicos/urina , Plantas Tóxicas/química , Cromatografia Líquida de Alta Pressão , Humanos , Espectrometria de Massas
14.
Se Pu ; 37(11): 1173-1178, 2019 Nov 08.
Artigo em Chinês | MEDLINE | ID: mdl-31642269

RESUMO

A rapid screening method was developed to determine sibutramine and five derivatives in health food by high performance liquid chromatography-quadrupole/electrostatic orbitrap high-resolution mass spectrometry (HPLC-Q/Orbitrap HRMS). The sample was extracted with methanol via ultrasonic-assisted extraction coupled with high-speed centrifugation. Separation was performed on a Hypersil Gold column (100 mm×2.1 mm, 3 µm) by gradient elution with methanol/water (containing 0.15%(v/v) formic acid) as the mobile phase. The positive full mass scan/date-dependent MS2 (Full MS/dd-MS2) mode was used during MS detection, and quantification was achieved by resolution of the precursor mass. The analytes in the sample were separated, and accurate mass and MS2 fragment ions were simultaneously attained within 8 min. The results indicated that the obtained mass accuracy errors of the six analytes were less than 1×10-6. Good linearities were obtained in the range of 0.5-20.0 µg/L, and all correlation coefficients were higher than 0.999. The limits of quantification were 25 µg/kg and the recoveries were in the range of 93.5%-103.5% with relative standard deviations of 1.5%-7.7%. This simple-pretreatment, rapid, accurate, high-sensitivity, and high-selectivity method can be used in the rapid screening and quantitative analysis of sibutramine and its derivatives in health food.


Assuntos
Ciclobutanos/análise , Análise de Alimentos/métodos , Cromatografia Líquida de Alta Pressão , Espectrometria de Massas , Eletricidade Estática
15.
Se Pu ; 37(11): 1241-1248, 2019 Nov 08.
Artigo em Chinês | MEDLINE | ID: mdl-31642279

RESUMO

A rapid method based on ultrahigh-performance liquid chromatography-quadrupole/electrostatic field orbitrap high-resolution mass spectrometry (UPLC-HRMS) was developed for the screening and confirmation of 20 mycotoxins in grain products. The samples were extracted with acetonitrile containing 2% (v/v) formic acid, and the extracts were cleaned up on Captive EMR-Lipid columns. The analytes were separated on a Thermo Hypersil Gold C18 column (100 mm×2.1 mm, 1.9 µm), and analyzed by UPLC-HRMS. The retention time and accurate mass of the parent ion were used for fast screening in full scan mode, while the accurate masses of the fragment ions were used for confirmation in the two-stage threshold-triggered full mass scan mode. The results revealed that the 20 mycotoxins showed good linear relationships in their respective mass concentration ranges. The correlation coefficients were not less than 0.99, and the limits of quantitation (LOQs) ranged from 0.25 to 20 µg/kg. The recoveries of the 20 mycotoxins in the sample ranged from 72.9% to 117.8% with the relative standard deviations (RSDs) from 2.9% to 15.2% at three spiked levels (n=6). This method has the advantages of high sensitivity and reliability, and is thus suitable for the rapid screening and confirmation of 20 mycotoxins in grain products.


Assuntos
Grão Comestível/química , Micotoxinas/análise , Cromatografia Líquida de Alta Pressão , Grão Comestível/microbiologia , Espectrometria de Massas , Reprodutibilidade dos Testes
16.
Braz Oral Res ; 33: e043, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31508727

RESUMO

Proteomic techniques have become popular in medicine and dentistry because of their widespread use in analyzing bodily fluids such as blood, saliva, urine, and gingival crevicular fluids as well as hard tissues such as enamel, dentine, and cementum. This review is a guide to proteomic techniques in general dentistry, summarizing techniques and their clinical application in understanding and diagnosing diseases and their use in identifying biomarkers of various diseases.


Assuntos
Proteoma , Proteômica/métodos , Saliva/química , Proteínas e Peptídeos Salivares/química , Biomarcadores/química , Eletroforese em Gel de Poliacrilamida/métodos , Humanos , Espectrometria de Massas/métodos , Neoplasias Bucais/diagnóstico , Síndrome de Sjogren/diagnóstico
17.
Yi Chuan ; 41(9): 863-874, 2019 Sep 20.
Artigo em Chinês | MEDLINE | ID: mdl-31549684

RESUMO

Membrane proteins play important functions not only as receptors and transporters, but also in many other important intracellular functions such as photosynthetic and respiratory electron transport. Identification of membrane proteins is a necessary step to understand their functions. Membrane proteins are generally highly hydrophobic and difficult to be resolved by aqueous solutions, and large-scale proteomic identification of membrane proteins has been a great technical challenge. Significant efforts have been invested in the field to improve the solubility of membrane proteins in aqueous solutions that are compatible for mass spectrometry analysis. This review summarizes the main technological achievements in the field of membrane proteomics particularly for the improvement of membrane protein identification, and uses the photosynthetic model cyanobacterium Synechocystis sp. PCC6803 as an example to illustrate how technology advances push forward the field in terms of the increased coverage of membrane proteome identification.


Assuntos
Proteoma , Proteômica/tendências , Synechocystis/genética , Proteínas de Bactérias/genética , Espectrometria de Massas
18.
Yi Chuan ; 41(9): 883-892, 2019 Sep 20.
Artigo em Chinês | MEDLINE | ID: mdl-31549686

RESUMO

Metabolomics (defined as comprehensive small molecule chemical analysis), together with genomics, transcriptomics, proteomics and phenomics, now plays a fundamental role in system biological studies. Chromatography- mass spectrometry machines, which have the characteristics of high resolution and high sensitivity, are widely used for metabolomics analysis, both qualitatively and quantitatively. With the fast development of the chromatography-mass spectrometry technology, metabolomics analysis has been successfully applied in various biological research fields. Here, we introduce the different chromatography-mass spectrum machines used for metabolomics analysis and their applications to various biological issues by mainly using the metabolomics platform in Institute of Genetics and Developmental Biology as a case study.


Assuntos
Metabolômica/instrumentação , Metabolômica/tendências , Cromatografia , Genômica , Espectrometria de Massas , Proteômica
19.
Gan To Kagaku Ryoho ; 46(9): 1377-1381, 2019 Sep.
Artigo em Japonês | MEDLINE | ID: mdl-31530774

RESUMO

Immune checkpoint blockades have brought about great benefits to cancer patient survival; however, the effect is yet limited and the overall objective rates are not satisfactory across cancer types. Understanding T cell discrimination against cancer and development of biomarkers are both indispensable for future application. In this brief review, we introduce proteogenomics HLA ligandome analysis, which combines conventional proteomics using mass spectrometry with genomic analysis data. The approach directly and comprehensively captures immunopeptidome of cancer cells, revealing an unprecedented number of antigens that can be targeted by T cells. Intriguingly, the repertoire comprises unique classes of peptides: neoantigens that arise from gene mutation, spliced peptides generated by the proteasomes, and non-coding region derived peptides. These findings demonstrate the diversity of T cell discrimination against cancer cells, and the accumulation of individual antigen data would contribute to development of precision medicine.


Assuntos
Neoplasias , Proteogenômica , Antígenos de Neoplasias , Humanos , Espectrometria de Massas , Medicina de Precisão
20.
An Acad Bras Cienc ; 91(3): e20180462, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31553365

RESUMO

This study aimed to evaluate the in vitro antiproliferative and inhibition of oxidative DNA-damage activities of n-butanol (n-BuOH) extract of Centaurea sphaerocephala. The in vitro antioxidant activity of the ethyl acetate (EtOAc) and the n-BuOH extracts of this plant were also assayed. To investigate the antioxidant potential, extracts were tested for their capacity to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH·) and to inhibit lipid peroxidation using the TBARs method. The contents of total phenolics and flavonoids were measured. Additionally, antiproliferative activity and DNA-damage inhibition of the n-BuOH extract was determined using XCELLigence RTCA instrument and photolyzing 46966 plasmid, respectively. The results exhibited that the scavenging abilities of the EtOAc extract were better than the n-BuOH extract with an IC50= 11.59 µg/mL and 16.67 µg/mL for both extracts, respectively. The phenolic and flavonoid contents were found higher in the n-BuOH and EtOAc extracts. Furthermore, our results showed that n-BuOH extract exhibited a remarkable inhibition of lipid peroxidation with an IC50 of 340.94±7.49 µg/mL and had an antiproliferative effect against Hela cells. Extracts of C. sphaerocephala showed antioxidant activity on scavenging DPPH·. In addition, the n-BuOH extract inhibited the lipid peroxidation and exhibited an antiproliferative effect against HeLa cells line (human cervix carcinoma).


Assuntos
1-Butanol/farmacologia , Acetatos/farmacologia , Antioxidantes/farmacologia , Proliferação de Células/efeitos dos fármacos , Centaurea/química , Dano ao DNA/efeitos dos fármacos , Extratos Vegetais/farmacologia , 1-Butanol/isolamento & purificação , Acetatos/isolamento & purificação , Antioxidantes/isolamento & purificação , Linhagem Celular Tumoral , Humanos , Espectrometria de Massas
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA