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1.
J Assoc Physicians India ; 67(10): 29-32, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31571448

RESUMO

Background: Data on pituitary Magnetic Resonance Imaging (MRI) in patients with abnormal pituitary hormones in Saudi Arabia are very scarce. Objective: To define the frequency of normal pituitary MRI in patients with abnormal pituitary hormones in a well-defined population. Design: Retrospective analysis of radiological and hormonal data of patients with pituitary MRI between January 2008 and December 2015. Settings: Departments of Endocrinology and Radiology at King Fahad Armed Forces Hospital, Jeddah, Saudi Arabia. Patients: 459 patients with clinical, hormonal and radiological data. Main outcome measures: The frequency of normal pituitary MRI in patients with abnormal pituitary hormones. Results: Over the 7-year period, Out of 459 patients; 129 (28.1 %) were males and 330 (71.9 %) were females with mean age of 35.4 ± 13.7. Positive MRI compared to normal MRI were seen in 268 (58.4 %) and 191 (41.6 %) subjects respectively. Subjects with Positive MRI were significantly older, 36.8 ± 14.1 vs. 33.5 ± 12.9, p value=0.01. Hyperfunctiong pituitary hormones were significantly associated with positive MRI, 259 (63.2%) vs. 151 (36.8) where as hypofunctiong pituitary hormones were associate with normal MRI, 40 (81.6%) vs. 9 (18.4%), p value < 0.001. Females with hyperfunctioning pituitary hormones were significantly associated with positive MRI whereas males with hyporfunctioning pituitary hormones were significantly associated with normal MRI. Three types of hyperfunctioning pituitary gland were seen such as hyperprolactinemia, somatotroph adenoma, and corticotroph adenoma were associated with more frequent positive MRI as to Five types of hypofunctioning pituitary gland were seen such as panhypopituitarism, secondary hypogonadism, growth hormone deficiency, central hypothyroidism and central adrenal insufficiency which were associated with more frequent normal MRI. Conclusion: The current study indicates hyperfunctioning pituitary gland was significantly associated with positive MR whereas hypofunctioning pituitary gland was associate with normal MRI. In the absence of registry data, larger cooperative studies involving diverse population samples from multiple centers could help to provide further information on the true frequency nationally. Limitations: Question of clustering of cases within the study region and limited study sample size.


Assuntos
Hipófise , Hormônios Hipofisários , Feminino , Humanos , Espectroscopia de Ressonância Magnética , Masculino , Estudos Retrospectivos , Arábia Saudita
2.
Arq. bras. cardiol ; 113(2 supl.1): 97-97, set., 2019.
Artigo em Português | Sec. Est. Saúde SP, SESSP-IDPCPROD, Sec. Est. Saúde SP | ID: biblio-1017200

RESUMO

FUNDAMENTO: A avaliação dos átrios de atletas e esportistas através da ressonância magnética foi pouco realizada até o presente momento, o que implica na necessidade do reconhecimento de padrões de normalidade dessas câmaras cardíacas para essa população. OBJETIVOS: realizar a avaliação morfofuncional biatrial em atletas e esportistas de endurance e compará-las com indivíduos sedentários e hígidos da mesma faixa etária e gênero; MÉTODOS: Estudo observacional e retrospectivo realizado no Instituto Dante Pazzanese de Cardiologia. Foram analisadas ressonâncias magnéticas de 25 atletas e esportistas, do gênero masculino, com idade entre 30 e 60 anos e comparadas com as de 10 sedentários, de mesmo gênero e faixa etária. Para ambos os átrios foram avaliados volumes diastólicos final (VDF) e sistólico final (VSF) e fração de ejeção, além da espessura de septo interatrial (SIA) e diâmetro anteroposterior do átrio esquerdo (AE). RESULTADOS: Apresentaram diferença estatística: VDF de átrio direito (AD) de 105,56 mL ±24,86 para atletas e 69,90 mL ±8,9 para sedentários... (AU)


Assuntos
Humanos , Masculino , Adulto , Pessoa de Meia-Idade , Espectroscopia de Ressonância Magnética , Atletas , Coração
3.
J Agric Food Chem ; 67(38): 10660-10666, 2019 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-31479263

RESUMO

Two new indolyl diketopiperazines, gartryprostatins A and B (1 and 2), with an unusual 2,3-furan-fused pyrano[2,3-g]pyrrolo[1″,2″:4',5']pyrazino[1',2':1,5]pyrrolo[2,3-b]indole nucleus, along with a new naturally occurring compound (gartryprostatin C, 3) were isolated from the solid culture of Aspergillus sp. GZWMJZ-258, an endophyte from Garcinia multiflora (Guttiferae). The structures of compounds 1-3 were determined by nuclear magnetic resonance, mass spectrometry, Marfey's analysis of amino acids, and chemical calculation. Compounds 1-3 displayed selective inhibition on human FLT3-ITD mutant AML cell line, MV4-11, with IC50 values of 7.2, 10.0, and 0.22 µM, respectively.


Assuntos
Aspergillus/química , Dicetopiperazinas/farmacologia , Endófitos/química , Garcinia/microbiologia , Plantas Medicinais/microbiologia , Aspergillus/genética , Aspergillus/isolamento & purificação , Aspergillus/metabolismo , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Dicetopiperazinas/química , Dicetopiperazinas/metabolismo , Endófitos/genética , Endófitos/isolamento & purificação , Endófitos/metabolismo , Humanos , Espectroscopia de Ressonância Magnética , Espectrometria de Massas , Estrutura Molecular
4.
Stud Health Technol Inform ; 267: 126-133, 2019 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-31483264

RESUMO

Magnetic Resonance Fingerprinting (MRF) is an imaging technique acquiring unique time signals for different tissues. Although the acquisition is highly accelerated, the reconstruction time remains a problem, as the state-of-the-art template matching compares every signal with a set of possible signals. To overcome this limitation, deep learning based approaches, e.g. Convolutional Neural Networks (CNNs) have been proposed. In this work, we investigate the applicability of Recurrent Neural Networks (RNNs) for this reconstruction problem, as the signals are correlated in time. Compared to previous methods based on CNNs, RNN models yield significantly improved results using in-vivo data.


Assuntos
Algoritmos , Redes Neurais (Computação) , Bases de Dados Genéticas , Espectroscopia de Ressonância Magnética
5.
Phys Chem Chem Phys ; 21(37): 20727-20742, 2019 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-31509121

RESUMO

The left-handed polyproline II (PPII) type helical structures are thought to play a very important role in many essential biological processes, particularly in recognition mechanisms. However, reliable characterisation of PPII conformation in solution can be experimentally challenging. Computational simulation of these structures offers an attractive alternative, but the accuracy of the results is dependent on the accuracy of the force field employed. In this report, we present the results of simulation of the structural and dynamical properties of a proline-rich viral fusion peptide for which a solution NMR study reported a substantial stretch of PPII conformation in the central region. The suggested mode of action of the p15 fusion peptide depended on the exposure of the flanking N-terminal hydrophobic residues to solvent thereby facilitating their interaction with the target membrane. Our simulations with a set of four force field and water model combinations consisting of (AMBER ff99SB*-ILDNP + TIP3P), (OPLS-AA + SPC/E), (AMBER ff03ws + TIP4P/2005 water with scaled protein-water interactions) and (CHARMM36m + TIP3P) showed a general agreement with the NMR results for all the four force field and water model combinations. The central region encompassing positions 9-15 showed a large PPII propensity, reduced flexibility and lower conformational entropy. The PPII conformations were stable and satisfied the Burgi-Dunitz criteria without the participation of any significant water bridging interaction. However, comparison of the experimentally observed chemical shifts with the distribution of shifts predicted from the simulated ensembles showed a much better agreement for the CHARMM36m + TIP3P and AMBER ff03ws + TIP4P/2005 combinations. The models based on these two force fields also generated conformations which were in much better agreement with the NMR model than the much more compact structures predicted by the AMBER ff99SB*-ILDNP and OPLS-AA force fields and predicted a substantially larger solvent accessible surface area in accordance with the suggested mechanism of action of the peptide.


Assuntos
Modelos Moleculares , Prolina/química , Proteínas Recombinantes de Fusão/química , Simulação por Computador , Espectroscopia de Ressonância Magnética , Conformação Molecular , Água/química
7.
Chem Commun (Camb) ; 55(66): 9869-9872, 2019 Aug 13.
Artigo em Inglês | MEDLINE | ID: mdl-31364617

RESUMO

In this work, we designed and synthesized an aggregation-induced emission (AIE)-active tetraphenylethene-decorated pseudo-trisialic acid (TPE3S) and validated its high affinity for Siglecs using microscale thermophoresis techniques. TPE3S was a unique binding-on fluorescent trivalent sialocluster which was successfully utilized for the visualization of Siglecs expressed on the surface of mammalian cells.


Assuntos
Metabolismo dos Carboidratos , Lectinas/metabolismo , Lectinas Semelhantes a Imunoglobulina de Ligação ao Ácido Siálico/metabolismo , Animais , Membrana Celular/metabolismo , Corantes Fluorescentes/química , Células HeLa , Humanos , Espectroscopia de Ressonância Magnética , Espectrometria de Massas , Células PC12 , Ligação Proteica , Ratos
8.
Zhonghua Xin Xue Guan Bing Za Zhi ; 47(7): 534-538, 2019 Jul 24.
Artigo em Chinês | MEDLINE | ID: mdl-31365993

RESUMO

Objective: To evaluate the clinical and cardiac magnetic resonance (CMR) features of apical hypertrophic cardiomyopathy (ApHCM) patients complicating with left ventricular apical aneurysm (LVAA). Methods: CMR confirmed 25 ApHCM patients complicating with LVAA from January 2010 to December 2017 in Fuwai hospital were included in this study, and the baseline clinical data and CMR characteristics were retrospectively analyzed. There were 14 pure ApHCM (hypertrophy limited at the apical segments) complicating with LVAA patients and 11 mixed ApHCM (predominantly apical hypertrophy along with thickening of contiguous non-apical left ventricular region) with LVAA patients. Results: In this patient cohort,age of 84% (21/25) patients ranged between 20-70 years old, and 68% (17/25) were male. There were 68% (17/25) patients with complaint of chest distress symptom, 56% (14/25) with complaint of chest pain, 32% (8/25) with complaint of palpitation,16% (4/25) with complaint of dyspnea, and 12% (3/25) presented as syncope. ST-T segment changes of electrocardiogram were observed in all patients, and giant negative T waves were detected in 80% patients (20/25). The rate of missed diagnosis by echocardiography for detecting ApHCM and LVAA was 16% (4/25) and 68% (17/25), respectively. CMR showed discrete thin-walled dyskinetic or akinetic segment of the most distal portion of the left ventricular chamber in ApHCM patients with LVAA. Transmural late gadolinium enhancement of the aneurysmal rim was detected in 76% (19/25) patients, and the maximum transverse dimension of aneurysm was bigger in patients with transmural late gadolinium enhancement than in patients without transmural late gadolinium enhancement ((22.0±10.8)mm vs. (11.7±4.0) mm, P=0.033). Conclusion: ApHCM with LVAA patients have distinct cardiac clinical features, and CMR is the most useful tool for the accurate and objective evaluation of this disease.


Assuntos
Cardiomiopatia Hipertrófica , Meios de Contraste , Adulto , Idoso , Eletrocardiografia , Feminino , Gadolínio , Humanos , Espectroscopia de Ressonância Magnética , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Adulto Jovem
9.
J Agric Food Chem ; 67(37): 10534-10542, 2019 Sep 18.
Artigo em Inglês | MEDLINE | ID: mdl-31464434

RESUMO

Fucosylated chondroitin sulfate from sea cucumber Stichopus japonicus (FCSSJ) has been demonstrated with various biological activities; however, its precise structure is still controversial, and digestive behavior remains poorly understood. FCSSJ was purified, and its detailed structure was elucidated mainly based on the NMR spectroscopic methods. Its main chain was characterized as →4)-ß-d-GlcA-(1 → 3)-ß-d-GalNAc-(1→ with GalNAc4S6S:GalNAc4S in a ratio of 1.5:1, and three types of sulfated fucosyl branches attaching C-3 of GlcA, namely, Fucp2S4S, Fucp3S4S, and Fucp4S, were found in a ratio of 2:1.5:1. The digestibility of FCSSJ was investigated in vitro, and the unchanged molecular weight and reducing sugar content indicated that FCSSJ was not broken down under salivary and gastrointestinal digestion. Furthermore, FCSSJ showed a significant inhibitory impact on pancreatic lipase dose-dependently but not on α-amylase, indicating that the inhibition of pancreatic lipase by FCSSJ might be a pathway for its hypolipidemic effect. These findings propose a fucosylated chondroitin sulfate and provide insight into the mechanism of its physiological effects in the digestion system.


Assuntos
Sulfatos de Condroitina/química , Sulfatos de Condroitina/metabolismo , Stichopus/química , Animais , Digestão , Trato Gastrointestinal/metabolismo , Humanos , Espectroscopia de Ressonância Magnética
11.
Phys Chem Chem Phys ; 21(35): 19209-19215, 2019 Sep 21.
Artigo em Inglês | MEDLINE | ID: mdl-31441478

RESUMO

Pure-shift NMR experiments provide highly resolved spectra, which could be perfect for precise monitoring of chemical shift variations under different conditions, such as temperature or concentration. However, their sensitivity is relatively low and signal sampling is time-consuming, which leads to long experimental times, making such serial acquisition problematic. In this paper we present a new method of NMR spectroscopy which improves the speed and sensitivity of serial pseudo-two-dimensional pure-shift experiments. The example of variable-temperature study of atorvastatin reveals the potential of the method in verifying the theoretical predictions of solvent-dependent spectral effects.


Assuntos
Atorvastatina/química , Técnicas de Química Analítica/métodos , Espectroscopia de Ressonância Magnética , Solventes/química , Temperatura Ambiente , Técnicas de Química Analítica/normas
12.
J Agric Food Chem ; 67(35): 9916-9925, 2019 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-31398025

RESUMO

Targeted analysis confirmed the presence of new phytosterol degradation products in fully hydrogenated commercial samples. EI-MS, APCI-MS, and 1D-NMR experiments led to the identification of 10 novel markers of catalytic hydrogenation, among which 5α-sitostan-3-one and 5α-campestan-3-one, isomers of saturated and monounsaturated steroidal hydrocarbons, were reported in edible oils for the first time. Examination of the phytosterol degradation mechanism was done by the catalytic transfer deuteration technique. The mitigation strategy of potentially detrimental compounds included optimization of processing parameters. The effect of catalyst dosage (≤0.1% based on Ni basis) and temperature region (>180 °C) were the most crucial factors in phytosterol degradation control.


Assuntos
Hidrocarbonetos/química , Óleos Vegetais/química , Catálise , Temperatura Alta , Hidrogenação , Isomerismo , Espectroscopia de Ressonância Magnética , Fitosteróis/química , Espectrometria de Massas por Ionização por Electrospray
14.
Chem Pharm Bull (Tokyo) ; 67(7): 693-698, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31257324

RESUMO

We aim to attain the sustainable use of longgu and have investigated the significance of longgu in Keishikaryukotsuboreito (KRB) decoction. We have already reported that longgu alters compound profiles in KRB decoction and hypothesized that it does so by adsorbing foreign organic compounds into its superficial pores. In the present study, we focused on the adsorbability of organic materials onto longgu surface as the cause of component profile alteration. We analyzed the physical changes in longgu through the decoction process by measuring the adsorbed water on longgu surface. 1H magic angle spinning NMR (1H-MASNMR) spectroscopic analysis revealed that raw longgu (R-raw) as well as decocted longgu [whether single (R-r) or KRB (R-krb) decoction] adsorbed water. However, the amount of adsorbed water in R-krb was smaller than that in R-raw and R-r. The nitrogen adsorption isotherms of longgu samples indicated that longgu was macroporous. The Brunauer-Emmett-Teller (BET) surface area of R-krb was smaller than that of R-raw and R-r. Further, thermogravimetric analysis of longgu samples showed that R-krb adsorbed matter that R-raw and R-r did not adsorb. The above findings and the 1H-MASNMR analysis of heated longgu samples suggested that longgu adsorbed organic compounds into the pores. We considered that longgu adsorbed organic compounds during KRB decoction into its superficial pores through the decoction process.


Assuntos
Medicamentos de Ervas Chinesas/química , Hipnóticos e Sedativos/química , Adsorção , Fósseis , Espectroscopia de Ressonância Magnética , Plantas Medicinais/química , Plantas Medicinais/metabolismo , Propriedades de Superfície , Termogravimetria
15.
Chem Commun (Camb) ; 55(66): 9797-9800, 2019 Aug 13.
Artigo em Inglês | MEDLINE | ID: mdl-31360962

RESUMO

Molecular tubes with hydrogen bonding donors in their deep hydrophobic cavities are able to selectively bind organophosphorus compounds in water through hydrogen bonding and the hydrophobic effect. They can also be used as a fluorescent sensor for nerve agent simulants and as an inhibitor to reduce the toxicity of paraoxon to acetylcholinesterase.


Assuntos
Acetilcolinesterase/efeitos dos fármacos , Inibidores da Colinesterase/química , Inibidores da Colinesterase/toxicidade , Agentes Neurotóxicos/química , Agentes Neurotóxicos/toxicidade , Paraoxon/química , Paraoxon/toxicidade , Calorimetria , Corantes Fluorescentes/química , Ligações de Hidrogênio , Interações Hidrofóbicas e Hidrofílicas , Espectroscopia de Ressonância Magnética/métodos , Termodinâmica , Água/química
16.
J Enzyme Inhib Med Chem ; 34(1): 1218-1225, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31286785

RESUMO

WaterLOGSY is a sensitive ligand-observed NMR experiment for detection of interaction between a ligand and a protein and is now well-established as a screening technique for fragment-based lead discovery. Here we develop and assess a protocol to derive ligand epitope mapping from WaterLOGSY data and demonstrate its general applicability in studies of fragment-sized ligands binding to six different proteins (glycogen phosphorylase, protein peroxiredoxin 5, Bcl-xL, Mcl-1, HSP90, and human serum albumin). We compare the WaterLOGSY results to those obtained from the more widely used saturation transfer difference experiments and to the 3D structures of the complexes when available. In addition, we evaluate the impact of ligand labile protons on the WaterLOGSY data. Our results demonstrate that the WaterLOGSY experiment can be used as an additional confirmation of the binding mode of a ligand to a protein.


Assuntos
Descoberta de Drogas/métodos , Espectroscopia de Ressonância Magnética/métodos , Sítios de Ligação , Proteínas/química
17.
Zhong Nan Da Xue Xue Bao Yi Xue Ban ; 44(5): 515-521, 2019 May 28.
Artigo em Chinês | MEDLINE | ID: mdl-31303614

RESUMO

Obstructive sleep apnea is often accompanied by functional changes in attention, alertness, long-term vision and language memory, visual space/structural competence, and executive function due to long-term intermittent hypoxia, high blood carbonate, and sleep structure disorders. In recent years, some scholars have found that the changes of brain structure, metabolism and function revealed by static functional magnetic resonance imaging (MRI) are closely related to the changes of cognitive function reflected in the cognitive function evaluation scale. In recent years, static functional magnetic resonance, especially voxel-based morphometry, diffusion tensor imaging, magnetic resonance spectroscopy and functional magnetic resonance imaging have confirmed significant changes in brain structure, metabolism and function in OSA patients, and the changes are closely related to the changes of cognitive function reflected in the cognitive function evaluation scale. It is great significance to study the mechanism of cognitive function change in OSA patients, and helpful to the early diagnosis and treatment as well as the evaluation of clinical efficacy.


Assuntos
Transtornos Cognitivos , Apneia Obstrutiva do Sono , Cognição , Imagem de Tensor de Difusão , Humanos , Espectroscopia de Ressonância Magnética , Apneia Obstrutiva do Sono/diagnóstico por imagem
18.
Phys Chem Chem Phys ; 21(28): 15630-15638, 2019 Jul 17.
Artigo em Inglês | MEDLINE | ID: mdl-31268447

RESUMO

We studied the influence of globotriaosylceramide (Gb3) lipid molecules on the properties of phospholipid membranes composed of a liquid ordered (lo)/liquid disordered (ld) phase separated 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC)/N-palmitoyl-d-erythro-sphingosylphosphorylcholine (PSM)/cholesterol mixture (40/35/20, mol/mol/mol) supplemented with 5 mol% of either short acyl chain palmitoyl-Gb3 or long acyl chain lignoceryl-Gb3 using 2H solid-state NMR spectroscopy. To this end, both globotriaosylceramides were chemically synthesized featuring a perdeuterated lipid acyl chain. The solid-state 2H NMR spectra support the phase separation into a POPC-rich ld phase and a PSM/cholesterol-rich lo phase. The long chain lignoceryl-Gb3 showed a rather unusual order parameter profile of the acyl chain, which flattens out for the last ∼6 methylene segments. Such an odd chain conformation can be explained by partial chain interdigitation and/or a very fluid midplane region of the membrane. Possibly, the Gb3 molecules may thus preferentially be localized at the lo/ld phase boundary. In contrast, the short chain palmitoyl-Gb3 was well associated with the PSM/cholesterol-rich lo phase. Gb3 molecules act as membrane receptors for the Shiga toxin (STx) produced by Shigella dysenteriae and by enterohemorrhagic strains of Escherichia coli (EHEC). The B-subunits of STx (STxB) forming a pentameric structure were produced recombinantly and incubated with the membrane mixtures leading to alterations in the lipid packing properties and lateral organization of the membranes. Typically, STxB binding led to a decrease in lipid chain order in agreement with partial immersion of protein segments into the lipid-water interface of the membrane. In the presence of STxB, Gb3 preferentially partitioned into the lo membrane phase. In particular the short acyl chain palmitoyl-Gb3 showed very similar chain order parameters to PSM. In the presence of STxB, all lipid species showed isotropic contributions to the 2H NMR powder spectra; this was most pronounced for the Gb3 molecules. Such isotropic contributions are caused by highly curved membrane structures, which have previously been detected as membrane invaginations in fluorescence microscopy. Our analysis estimated that STxB induced highly curved membrane structures with a curvature radius of less than ∼10 nm likely related to the insertion of STxB segments into the lipid-water interface of the membrane.


Assuntos
Bicamadas Lipídicas/química , Lipídeos/química , Espectroscopia de Ressonância Magnética , Toxina Shiga/química , Ligação Proteica
19.
J Agric Food Chem ; 67(31): 8520-8526, 2019 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-31310120

RESUMO

Reactive carbonyl species (RCS), such as acrolein (ACR), glyoxal (GO), and methylglyoxal (MGO), have received extensive attention recently as a result of their high activity and toxicity in vitro and in vivo. In the present study, propyl gallate (PG), a common food antioxidant, was found to effectively trap more ACR than butylated hydroxytoluene and butylated hydroxyanisole through the formation of mono-ACR adducts (PG-ACR) and di-ACR adducts (PG-2ACR). The two adducts were successfully purified, and their structures were elucidated on the basis of their high-resolution mass spectrometry and 1H, 13C, and two-dimensional nuclear magnetic resonance data. We further identified that PG-ACR had the ability to continue to trap GO and MGO to form PG-ACR-GO and PG-ACR-MGO, respectively, by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Furthermore, we verified that PG could inhibit the production of ACR, GO, and MGO via trapping these RCS simultaneously to form the corresponding adducts in pound cakes using LC-MS/MS.


Assuntos
Acroleína/química , Antioxidantes/química , Galato de Propila/química , Culinária , Temperatura Alta , Espectroscopia de Ressonância Magnética , Modelos Biológicos , Estrutura Molecular , Espectrometria de Massas em Tandem
20.
J Agric Food Chem ; 67(31): 8425-8430, 2019 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-31322874

RESUMO

In recent years, non-targeted methods have been a popular "buzz" phrase in food fraud detection. Using analytical instrumentation techniques, non-targeted methods have been developed and applied in many food and agricultural situations. However, confusion and misstatements remain regarding how the methods are used. This perspective will discuss the definitions related to non-targeted testing, the procedure of developing and validating methods, the techniques and data analysis, and opportunities and challenges regarding the use of this class of analytical methods. The perspective seeks to provide readers with the latest information regarding recent advances in the use of non-targeted methods.


Assuntos
Análise de Alimentos/métodos , Contaminação de Alimentos/análise , Cromatografia Líquida/métodos , Eletroforese Capilar/métodos , Análise de Alimentos/instrumentação , Humanos , Espectroscopia de Ressonância Magnética/métodos , Espectrometria de Massas/métodos
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