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1.
J Prosthodont ; 26(1): 19-28, 2017 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-26883670

RESUMO

PURPOSE: Chronic inflammatory disorders of the oral cavity, such as periodontitis, were recently linked to systemic immune activation. Since fungal oral infections have not yet been studied in this respect, the aim of our study is to determine whether the local inflammation caused by oral fungal infection of the palatal tissue (denture stomatitis-DS) is associated with the systemic inflammatory response. This question is becoming essential as the population ages. MATERIALS AND METHODS: Peripheral blood of DS patients (n = 20) and control patients (n = 24) was assessed with flow cytometry to determine lymphocyte and monocyte profiles. Intracellular cytometric analysis was carried out to establish cytokine production by T cells. DS was diagnosed based on clinical symptoms of DS such as swelling and redness of oral mucosa, confirmed by microbiological swabs for fungal colonization with Candida species. The control group was recruited from denture users without clinical and microbiological signs of oral infections. RESULTS: Percentages of peripheral lymphocytes, T cells, monocytes, and their subpopulations were similar in both studied groups. The exception was median percentages of CD25+ T cell subsets, which were significantly lower in DS patients than in control subjects. This reduction was observed in both CD4 T cell subset (16.7% and 28.1%; p = 0.0006) and CD8 T cell subset (4.6% and 7.0%; p = 0.007) CONCLUSIONS: While DS and associated local fungal infection do not overtly affect activation of monocytes or lymphocytes, the number of CD 25+ T cells is significantly lower in the DS patients, possibly indicating limited potential for the infection clearance in denture-using aging patients.


Assuntos
Subunidade alfa de Receptor de Interleucina-2/metabolismo , Estomatite sob Prótese/imunologia , Subpopulações de Linfócitos T/metabolismo , Idoso , Candidíase Bucal/imunologia , Estudos de Casos e Controles , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estomatite sob Prótese/microbiologia , Subpopulações de Linfócitos T/imunologia
2.
Cytokine ; 77: 145-51, 2016 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-26587801

RESUMO

Candida-associated denture stomatitis (DS) is the most frequent lesion among denture wearers, especially the elderly. DS is strongly associated with Candida albicans, as well as local and systemic factors, such as impaired immune response. Monocytes are important in the protective immune response against the fungus by the production of cytokines that recruit and activate leukocytes. There are functional changes in these cells with age, and individual alterations involving monocyte response may predispose the host to developing infections by Candida spp. In this study, our aim was to evaluate the production of TNF-α, IL-6, CXCL8, IL-1ß, MCP-1 and IL-10 by monocytes from elderly denture wearers with/without DS and elderly or young non-denture wearers. We detected that monocytes from elderly denture wearers with Candida-related denture stomatitis produced lower levels of CXCL-8, IL-6 and MCP-1. This imbalance in cytokine levels was observed in spontaneous or LPS-stimulated production. Therefore, our data suggested that inherent aspects of the host, such as changes in cytokine production by monocytes, might be associated with the development and the persistence of DS irrespective of aging.


Assuntos
Candida/imunologia , Candidíase/imunologia , Citocinas/imunologia , Mediadores da Inflamação/imunologia , Monócitos/imunologia , Estomatite sob Prótese/imunologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Candida/classificação , Candida/fisiologia , Candida albicans/imunologia , Candida albicans/fisiologia , Candida tropicalis/imunologia , Candida tropicalis/fisiologia , Candidíase/metabolismo , Candidíase/microbiologia , Quimiocina CCL2/imunologia , Quimiocina CCL2/metabolismo , Citocinas/metabolismo , Ensaio de Imunoadsorção Enzimática , Feminino , Interações Hospedeiro-Patógeno/imunologia , Humanos , Mediadores da Inflamação/metabolismo , Interleucina-6/imunologia , Interleucina-6/metabolismo , Interleucina-8/imunologia , Interleucina-8/metabolismo , Masculino , Pessoa de Meia-Idade , Monócitos/metabolismo , Monócitos/microbiologia , Especificidade da Espécie , Estomatite sob Prótese/metabolismo , Estomatite sob Prótese/microbiologia
3.
Gerodontology ; 32(2): 132-40, 2015 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-23829887

RESUMO

OBJECTIVES: This study investigated risk factors of denture stomatitis, and the levels of cytokines in the saliva of elderly Candida-related denture stomatitis participants compared with adult individuals. MATERIAL AND METHODS: The occurrence of denture stomatitis in 128 patients with upper removable dentures was clinically examined. Participants were divided into two age groups as adult and elder. Risk factors associated with denture stomatitis were evaluated by questionnaire and oral and dental prosthesis examination. The quality of dentures was evaluated by direct examination. Palatal mucosa was swabbed for Candida carriage investigation, and whole unstimulated saliva was collected for cytokine detection. Salivary cytokines (interleukin (IL)-6, IL-8, IL-10, IL-17, intercellular adhesion molecule (ICAM)-1 and tumour necrosis factor (TNF)-α) were examined by enzyme-linked immunosorbent assay (ELISA). RESULTS: The associations between the occurrence of denture stomatitis and either the quality of dentures or Candida isolation on palatal mucosa were significant. However, differences in the levels of salivary IL-6, IL-8, IL-10, IL-17, ICAM-1 and TNF-α between the denture wearers with and without denture stomatitis were undetectable. Adult and elderly Candida-related denture stomatitis patients also showed a similar level of salivary IL-6, IL-8, IL-10, IL-17, ICAM-1 and TNF-α. No correlation between the presence of denture stomatitis in the elder and the quantity of Candida infection was found. CONCLUSION: No association was found between the occurrence of Candida-related denture stomatitis and the concentrations of salivary IL-6, IL-8, IL-10, IL-17, ICAM-1 and TNF-α, regardless of age.


Assuntos
Candida/imunologia , Citocinas/imunologia , Saliva/imunologia , Estomatite sob Prótese/imunologia , Estomatite sob Prótese/microbiologia , Adulto , Fatores Etários , Idoso , Idoso de 80 Anos ou mais , Candida/isolamento & purificação , Candidíase Bucal/imunologia , Candidíase Bucal/microbiologia , Dentaduras/efeitos adversos , Dentaduras/microbiologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Fatores de Risco
4.
Artigo em Inglês | MEDLINE | ID: mdl-24055150

RESUMO

OBJECTIVE: This study investigated the susceptibility of 198 clinical isolates of Candida species against caspofungin, amphotericin B, itraconazole, and fluconazole. STUDY DESIGN: Suspensions of the microorganisms were spread on Roswell Park Memorial Institute (RPMI) agar plates. Etest strips were placed on the plates, and the minimal inhibitory concentration (MIC) was read after incubation (48 h at 37 °C). Data were analyzed by a factorial analysis of variance and a 2 × 2 post hoc test (α = .05). RESULTS: C glabrata showed the highest MIC values (P < .001) against caspofungin, itraconazole, and fluconazole. For amphotericin B, the MIC values of C tropicalis and C glabrata (P = .0521) were higher than those of C albicans (P < .001). Itraconazole was the least effective antifungal; 93.3% of the C glabrata isolates, 3.3% of the C albicans, and 1.3% of the C tropicalis were resistant. All microorganisms were susceptible to caspofungin and amphotericin B. CONCLUSIONS: Caspofungin and amphotericin B should be recommended as an effective alternative for the management of oral Candida infections when treatment with topical or other systemic drugs has definitely failed.


Assuntos
Antifúngicos/farmacologia , Candida/efeitos dos fármacos , Candidíase Bucal/microbiologia , Diabetes Mellitus/imunologia , Estomatite sob Prótese/imunologia , Estomatite sob Prótese/microbiologia , Anfotericina B/farmacologia , Brasil , Candida/classificação , Candida/isolamento & purificação , Caspofungina , Farmacorresistência Fúngica , Equinocandinas/farmacologia , Fluconazol/farmacologia , Humanos , Itraconazol/farmacologia , Lipopeptídeos , Testes de Sensibilidade Microbiana
5.
Bauru; s.n; 2013. 148 p. ilus, tab, graf.
Tese em Português | LILACS, BBO - Odontologia | ID: lil-719057

RESUMO

A presença de Candida albicans nos biofilmes microbianos da superfície interna das próteses totais superiores está relacionada com uma doença inflamatória no palato, a estomatite protética. Constituinte da defesa inata do hospedeiro, o epitélio bucal, por sua vez, tem a capacidade de reconhecer e reagir aos fatores fúngicos a fim de evitar a invasão pelo microrganismo. O objetivo deste trabalho foi avaliar in vitro o efeito direto e indireto de C. albicans viável sobre as células epiteliais de palato humano (CEPH) ao longo do tempo. Objetivamos correlacionar os eventos de agressão, apoptose e invasão das CEPH provocados pelo fungo, com as respostas de defesa epitelial mediante produção de óxido nítrico (NO) e expressão gênica do peptídeo antimicrobiano β-defensina 2 (hBD-2). Material e Métodos: As CEPH foram obtidas, parte pelo método explante e parte pelo método enzimático, e mantidas em co-cultivo sobre uma camada de sustentação feederlayer (fibroblastos gengivais humanos mitoticamente inativados). Após desafios das CEPH com C. albicans ATCC 90028 por contato direto fungo-epitélio (D.D.) e indireto pelo sobrenadante da cultura do fungo hifal (D.I.), proporções de desafio de 0,01/1; 0,025/1 e 0,1/1 levedura/queratinócito (FUN/EPI) e tempos experimentais de 3, 6 e 10 h foram determinados; via ensaios de viabilidade celular por imunofluorescência (LIVE/DEAD), e análise qualitativa da invasão celular pelo fungo por meio do método colorimétrico com laranja de acridina. A apoptose epitelial foi determinada pela marcação nuclear fluorescente com Hoechtst 33258. A produção de óxido nítrico (NO) e a expressão de RNAm de hBD-2 foram avaliados por reação colorimétrica de Griess e RT-qPCR, respectivamente. Os resultados foram expressos como média ± desvio padrão e submetidos aos testes estatísticos ANOVA Fatorial, Teste de Contraste; ou Teste de Mann-Whitney (p<0,05). Resultados: Em 3 h, foi detectado aumento da apoptose das células epiteliais em relação ao...


The presence of the fungus Candida albicans in the microbial biofilm underlying maxillary prosthesis is related to an inflammatory reaction of the palatal mucosa, the denture stomatitis. As a component of the host innate defense, the oral epithelium has the ability to recognize and react to fungal factors in order to prevent the microrganism invasion. The aim of this study was to in vitro evaluate the direct and indirect effect of viable C. albicans on the human palatal epithelial cells (HPEC) over time. The aggressive events, such as apoptosis and HPEC invasion by the fungus, were correlated with epithelial defense responses through the nitric oxide (NO) production and antimicrobial peptides β-defensin (hBD-2) mRNA expression. Methods: The HPEC were obtained by explant and enzymatic methods, and were maintained in co-culture on a feeder-layer support (mitotically inactivated human gingival fibroblasts). After the HPEC challenges with C. albicans ATCC 90028 by direct contact fungus-epithelium (D.D.) and indirect contact by supernatant from hyphal fungus (D.I.), defiance ratios of 0.01/1, 0.025/1 and 0.1/1 yeast/keratinocyte (FUN/EPI) and experimental times of 3, 6 and 10 h were determined. These conditions were standardized by cell viability immunofluorescence assay (LIVE/DEAD), and cell invasion qualitative analysis (colorimetric method with acridine orange). The apoptotic cells were determined by fluorescent nuclear staining with Hoechtst 33258. The nitric oxide (NO) production and hBD-2 gene expression were evaluated by Griess colorimetric reaction and RT-qPCR, respectively. The results were expressed as mean ± standard deviation and were analyzed using the factorial ANOVA, Contrast Test; or Mann-Whitney Test (p<0,05). Results: At 3 h, the apoptotic epithelial cells under 0.1/1 FUN/EPI increased compared to epithelium unchallenged (p<0,05) that remained over time with increasing concentration and independent of D.D. and D.I. The onset...


Assuntos
Humanos , Candida albicans/crescimento & desenvolvimento , Células Epiteliais/imunologia , Células Epiteliais/microbiologia , Estomatite sob Prótese/imunologia , Estomatite sob Prótese/microbiologia , Apoptose , Sobrevivência Celular , Células Cultivadas , Imunidade nas Mucosas , Mucosa Bucal/imunologia , Mucosa Bucal/microbiologia , Palato/imunologia , Palato/microbiologia , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Fatores de Tempo
6.
Gen Dent ; 60(6): 494-500; quiz p. 501-2, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-23220304

RESUMO

Periodontitis is a primarily bacterial infection that is common in dentate individuals, while denture stomatitis is a predominantly fungal infection that is common among denture wearers. Both infections may increase a patient's risk for chronic systemic infection dissemination, and may in turn increase the risk of chronic, inflammatory-based systemic diseases. Systemic diseases for which chronic oral infections are believed to confer attributable risk include atherosclerotic and coronary disease, stroke, chronic obstructive pulmonary disease, diabetes, and hypertension. It appears that invasive oral pathogens trigger a systemic inflammatory response via mediators released by the cardiovascular system and liver, putting the patient at increased risk for these diseases. Data comparing gene expression between denture wearers with and without denture stomatitis (and associated Candida albicans infections) has demonstrated unique up- and down-regulation patterns for a number of genes. It appears that down-regulated genes (whose functions are thereby diminished) are associated with reduced epithelial barrier integrity. By contrast, there appears to be an association between up-regulated genes (which have enhanced function) and inflammatory responses that facilitate the ability of C. albicans to bind with and penetrate the oral mucosa. Molecular biological approaches suggest that future therapeutic development could target reducing either the local inflammatory processor, the binding and attachment of C. albicans to the oral mucosa, or both. Ongoing investigations are attempting to incorporate interventions into matrices, to provide a local and sustained presence to therapeutic interventions.


Assuntos
Nível de Saúde , Boca Edêntula/microbiologia , Saúde Bucal , Candidíase Bucal/imunologia , Doença Crônica , Infecção Focal Dentária/imunologia , Regulação da Expressão Gênica/genética , Humanos , Boca Edêntula/imunologia , Periodontite/imunologia , Periodontite/microbiologia , Fatores de Risco , Estomatite sob Prótese/imunologia , Estomatite sob Prótese/microbiologia
7.
Mol Biosyst ; 8(12): 3216-23, 2012 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-23041753

RESUMO

Denture stomatitis (DS) is the most common oral pathology among denture wearers, affecting over one-third of this group. DS is usually associated with C. albicans. However, unlike other oral candidiasis, most DS patients have intact host immunity. The presence of a denture alone is usually sufficient for DS. Saliva and its protein contents can theoretically predispose some denture wearers to DS and others resistant toward DS. Here we proposed for the first time to define salivary proteomic profiles of denture wearers with and without DS. SELDI-TOF/MS analysis suggests that there is a proteomic differentiation among control, localized and generalized DS. Based on initial SELDI-TOF/MS profiling, we further used reversed phase liquid chromatography, MALDI-TOF/MS, and LC-MS/MS to characterize the salivary proteins associated with DS. Nineteen proteins based on SELDI-TOF/MS profiling were found including cystatin-SN, statherin, kininogen-1, desmocollin-2, carbonic anhydrase-6, peptidyl-prolyl cis-trans isomerase A like peptides, cystatin C, and several immunoglobulin fragments. The proteomic content gives evidence of the interaction between host tissue, saliva, and candida. Further examination in larger populations of these proteins may help to gain a better understanding of DS pathological processes and improve DS treatments.


Assuntos
Dentaduras/efeitos adversos , Proteínas e Peptídeos Salivares/metabolismo , Estomatite sob Prótese/etiologia , Estomatite sob Prótese/metabolismo , Idoso , Candida albicans/imunologia , Candida albicans/metabolismo , Candida albicans/patogenicidade , Candidíase Bucal/imunologia , Candidíase Bucal/metabolismo , Feminino , Humanos , Imunoglobulinas/análise , Masculino , Pessoa de Meia-Idade , Análise Serial de Proteínas , Proteômica , Saliva/química , Saliva/imunologia , Saliva/metabolismo , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Estomatite sob Prótese/imunologia , Estomatite sob Prótese/microbiologia
8.
Gerodontology ; 29(2): e331-9, 2012 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-21453417

RESUMO

OBJECTIVES: Elderly individuals with Candida-related denture stomatitis (DS) present with a reduced defence against Candida albicans. This study evaluated levels of antimicrobial mediators in the elderly DS saliva and salivary neutrophils' activation characteristics compared with elderly and young without DS. METHODS: Salivary peroxidases (SPO) and elastase activities (ELA), nitric oxide (NO), transforming growth factor beta (TGF-ß), IL-6 and CCL3 production were determined in saliva from elderly with or without DS, and young control individuals. TLR4, CXCR1, CD11b, CD16 and CD32 expression on salivary neutrophils were evaluated. Correlations between number and apoptosis rate of salivary neutrophils, enzymatic activities and cytokine levels were determined. RESULTS: Elderly DS individuals exhibited the lowest SPO and ELA activities. Also, the activity of both enzymes was low in elderly without DS. Although both elderly groups showed higher salivary NO and TGF-ß levels compared to young control groups, elderly DS presented the highest salivary NO, TGF-ß, IL-6 and CCL3 levels. Decreased percentages of salivary TLR4(+) and CD16(+) neutrophils were detected in both elderly groups. Although these damages could influence the establishment and persistence of DS, the highest levels of salivary IL-6 and CCL3 in elderly DS could be preventing more serious complications.


Assuntos
Candidíase Bucal/imunologia , Saliva/imunologia , Estomatite sob Prótese/imunologia , Adulto , Idoso , Anti-Infecciosos/análise , Antígeno CD11b/análise , Candida albicans/imunologia , Quimiocina CCL3/análise , Proteínas Ligadas por GPI/análise , Humanos , Mediadores da Inflamação/análise , Interleucina-6/análise , Elastase de Leucócito/análise , Pessoa de Meia-Idade , Ativação de Neutrófilo/imunologia , Neutrófilos/imunologia , Óxido Nítrico/análise , Peroxidases/análise , Receptores de IgG/análise , Receptores de Interleucina-8A/análise , Saliva/química , Proteínas e Peptídeos Salivares/análise , Receptor 4 Toll-Like/análise , Fator de Crescimento Transformador beta/análise , Adulto Jovem
9.
Eur J Clin Microbiol Infect Dis ; 31(6): 1271-7, 2012 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-22120419

RESUMO

We have identified impaired neutrophils in elderly individuals which could be involved with Candida-related denture stomatitis (DS), an oral infection predominantly caused by Candida albicans, affecting especially elderly individuals using dental prosthesis. However, specific mechanisms performed by neutrophil contributing to the susceptibility of the elderly to DS are not fully understood. This study evaluated activation features of blood neutrophils from elderly and young individuals with DS. Blood neutrophils cultured with C. albicans from elderly subjects secreted decreased levels of CXCL8. However, C. albicans challenged-neutrophils from DS patients produced high IL-4 and IL-10, and low GM-CSF levels, regardless of age. Additional elastase activity of neutrophils from both elderly groups was detected after incubation with C. albicans, but only neutrophils from elderly DS demonstrated high myeloperoxidase activity. Therefore, DS patients have affected neutrophils, and the advance of age intensifies these damages. In summary, individuals with Candida-related denture stomatitis presented variation in the neutrophil phenotype and activation. Such alterations were more intense in neutrophils from infected elderly individuals.


Assuntos
Sangue/imunologia , Candida albicans/imunologia , Candidíase Bucal/imunologia , Ativação de Neutrófilo , Estomatite sob Prótese/imunologia , Adulto , Fatores Etários , Idoso , Idoso de 80 Anos ou mais , Candida albicans/patogenicidade , Células Cultivadas , Citocinas/metabolismo , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Elastase Pancreática/metabolismo , Peroxidase/metabolismo
10.
Curr Opin Microbiol ; 14(4): 380-5, 2011 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-21741878

RESUMO

Biofilms are microbial communities that form on surfaces and are embedded in an extracellular matrix. C. albicans forms pathogenic mucosal biofilms that are evoked by changes in host immunity or mucosal ecology. Mucosal surfaces are inhabited by many microbial species; hence these biofilms are polymicrobial. Several recent studies have applied paradigms of biofilm analysis to study mucosal C. albicans infections. These studies reveal that the Bcr1 transcription factor is a master regulator of C. albicans biofilm formation under diverse conditions, though the most relevant Bcr1 target genes can vary with the biofilm niche. An important determinant of mucosal biofilm formation is the interaction with host defenses. Finally, studies of interactions between bacterial species and C. albicans provide insight into the communication mechanisms that endow polymicrobial biofilms with unique properties.


Assuntos
Biofilmes , Candida albicans/patogenicidade , Interações Hospedeiro-Patógeno , Membrana Mucosa/microbiologia , Candida albicans/genética , Candida albicans/imunologia , Candida albicans/fisiologia , Candidíase Bucal/imunologia , Candidíase Bucal/microbiologia , Candidíase Vulvovaginal/microbiologia , Feminino , Regulação Fúngica da Expressão Gênica , Genes Fúngicos , Humanos , Imunidade Inata , Membrana Mucosa/imunologia , Estomatite sob Prótese/imunologia , Estomatite sob Prótese/microbiologia , Fatores de Transcrição/metabolismo
11.
Bauru; s.n; 2011. 135 p. ilus, tab, graf.
Tese em Português | LILACS, BBO - Odontologia | ID: biblio-865844

RESUMO

Estomatite protética associada a Cândida (EPC), a lesão mais frequente em usuários de próteses removíveis, principalmente os idosos, caracteriza-se por uma inflamação da mucosa bucal que suporta a prótese. Está fortemente associada com Candida Albicans, bem como com fatores locais e sistêmicos, como a deficiência da resposta imune. Os monócitos são importantes na resposta protetora contra o fungo, produzindo citocinas que recrutam e ativam leucócitos. Existem alterações funcionais dessas células com o avanço da idade. Não foi possível encontrar na literatura dados referentes à função imunomodulatória dos monócitos de idosos com EPC. O presente trabalho pretendeu avaliar a produção de citocinas por essas células, estimuladas in vitro com C. albicans, obtidas do sangue periférico de idosos usuários de prótese total superior (PTS) com EPC, comparando-se com idosos usuários de PTS sem EPC, e com idosos e jovens não usuários de PTS. Os monócitos isolados foram cultivados em placas de cultura de 24 poços, na ausência ou presença de lipopolissacarídeo (LPS) ou C. albicans ATCC 90028 morta pelo calor. Após 18 horas, o sobrenadante foi coletado e submetido ao ensaio de imunoabsorção por ligação enzimática (ELISA) para dosagem das citocinas pró- inflamatórias fator de necrose tumoral- (TNF-), interleucina-6 (IL-6), IL-1, CXCL8 e proteína quimiotática de monócito (MCP-1), e anti-inflamatórias IL-10 e fator transformador de crescimento- (TGF-). Os resultados estão expressos como média ± desvio padrão dos valores obtidos para cada grupo, e foram analisados por meio de testes estatísticos não-paramétricos. Valores de p<0,05 foram considerados estatisticamente significativos. Os resultados demonstraram, de uma forma geral, alterações nos monócitos oriundos dos idosos com EPC, em comparação aos outros grupos: menor produção espontânea de CXCL8 e de MCP-1; menores níveis de TNF-, de IL-6, de IL-1, de CXCL8, de MCP-1 e de IL-10, após estímulo com LPS; e menor...


Candida-associated denture stomatitis (DS), the most frequent lesion among denture wearers, especially the elderly, is characterized by inflammation of the denture-bearing mucosa. It is strongly associated with Candida albicans, as well as with local and systemic factors, such as impaired immune response. Monocytes are important in the protective immune response against the fungus, by the production of cytokines that recruit and activate leukocytes. There are functional changes of these cells with advancing age. No data were found in the literature concerning the immunomodulatory function of these phagocytes in elderly patients with DS. This study aimed to evaluate the cytokine production by monocytes, challenged in vitro with C. albicans, obtained from peripheral blood of elderly denture wearers with DS, compared with elderly denture wearers without DS and elderly and young non-denture wearers. The isolated monocytes were cultivated in 24-well flat-bottomed culture plates, in the absence or presence of lipopolysaccharide (LPS) or heat-killed C.albicans ATCC 90028. After 18 hours, the supernatant was collected and submitted to the enzyme-linked immunosorbent assay (ELISA) for determination of the pro-inflammatory cytokines tumor necrosis factor-alpha (TNF-), interleukin-6 (IL-6), CXCL8, IL-1, monocyte chemotactic protein-1 (MCP-1) and anti-inflammatory cytokines IL-10 and transforming growth factor- (TGF-). The results are expressed as mean ± standard deviation of the values obtained for each group, and were analyzed using nonparametric statistical tests; p values <0.05 were considered statistically significant. The results demonstrated, in general, changes in monocytes from the elderly with DS, as compared to other groups: lower spontaneous production of CXCL8 and MCP-1; lower levels of TNF-, IL-6, IL-1, CXCL8, MCP-1 and IL-10 after stimulation with LPS; and reduced production of TNF- and IL-6 after stimulation with C. albicans. Comparing...


Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Idoso , Idoso de 80 Anos ou mais , Candida albicans/imunologia , Citocinas/biossíntese , Estomatite sob Prótese/imunologia , Monócitos/metabolismo , Fatores Etários , Ensaio de Imunoadsorção Enzimática , Imunofluorescência , Prótese Total Superior/microbiologia
13.
Eur J Prosthodont Restor Dent ; 15(2): 50-4, 2007 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-17645064

RESUMO

Denture stomatitis is an inflammatory condition affecting palatal mucosa covered by a denture. Aetiology is multifactorial involving poor hygiene, constant denture wearing, trauma and colonisation by Candida species. In inflamed tissues raised levels of albumin, IgG isotypes and secretory IgA (sIgA) would be expected. SIgA is a major component of the protective immunological response to mucosal injection. Palatal secretions of 21 patients with denture stomatitis and 22 denture wearing patients with clinically healthy palates were analysed for albumin, IgG isotypes and sIgA levels. Albumin and IgG isotype levels were found to be higher in denture stomatitis patients compared with healthy palate patients. Unexpectedly, SIgA levels were found to be significantly (p<0.05) lower in denture stomatitis patients compared with healthy palate patients.


Assuntos
Imunoglobulina A Secretora/análise , Imunoglobulina G/análise , Palato/metabolismo , Saliva/imunologia , Estomatite sob Prótese/imunologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Albuminas/análise , Métodos Epidemiológicos , Feminino , Humanos , Masculino , Pessoa de Meia-Idade
14.
Stomatologiia (Mosk) ; 85(4): 37-40, 2006.
Artigo em Russo | MEDLINE | ID: mdl-17047610

RESUMO

In blood and the mixed saliva of patients with intolerance of stomatologic constructional materials the maintenance of cytokines, factors of humoral answer were investigated. It was established, that in formation of intolerance the increased sensitivity stomatologic constructional materials which is not connected to allergic reaction of immediate type and is, apparently, pseudoallergic or develops on other type. There is an activation of the local immune answer including local synthesis of proinflammatory cytokines, sIgA and hyperactivation of complement system to formation of C3a component. Toxic and allergic action of stomatologic constructional materials grows in patients having antibodies to Candida albicans in the mixed saliva.


Assuntos
Complemento C3a/metabolismo , Citocinas/metabolismo , Dentaduras/efeitos adversos , Imunoglobulina A/metabolismo , Saliva/metabolismo , Estomatite sob Prótese/imunologia , Adulto , Idoso , Anticorpos Anti-Idiotípicos/imunologia , Biomarcadores/metabolismo , Humanos , Imunoglobulina A/imunologia , Pessoa de Meia-Idade , Saliva/imunologia , Estomatite sob Prótese/etiologia , Estomatite sob Prótese/metabolismo
15.
Mikrobiol Z ; 66(1): 57-61, 2004.
Artigo em Ucraniano | MEDLINE | ID: mdl-15104056

RESUMO

Microflora of oral cavity of people of 45-65 years with removable denture and children with acute herpetic stomatitis, its dependence upon the degree of local immunity, presence and severity of inflammatory process have been studied. Percentage of leucocyte subpopulations activity of bactericide enzymes of granulocytes, average anticolonization ratio, correlation of Gram-positive and Gram-negative bacteria, degree of colonization with Candida fungi have been determined is smear-simprints of mucous membrane. The intensity of growth of optional anaerobic microorganisms has been investigated in bacteriological studies of material taken from mucous membrane. Their identification has been performed using morphological-cultural and biochemical indices. Significant changes in microbiocenosis of oral cavity such as disappearance of autochthonous microflora, appearance and intensive growth of pathogenic and opportunistic microorganisms are to be observed in conditions of decrease of non-specific resistance and presence of inflammatory process in persons with removable denture and acute herpetic stomatitis.


Assuntos
Mucosa Bucal/microbiologia , Estomatite sob Prótese/microbiologia , Estomatite Herpética/microbiologia , Doença Aguda , Idoso , Anaerobiose , Candida/isolamento & purificação , Criança , Bactérias Gram-Negativas/isolamento & purificação , Bactérias Gram-Positivas/isolamento & purificação , Humanos , Contagem de Leucócitos , Pessoa de Meia-Idade , Mucosa Bucal/imunologia , Estomatite sob Prótese/imunologia , Estomatite Herpética/imunologia
16.
Acta odontol. venez ; 40(3): 32-36, dic. 2002.
Artigo em Espanhol | LILACS | ID: lil-355238

RESUMO

La estomatitis subprotÚsica (E.S.P.) es una entidad que se localiza principalmente en la mucosa del paladar que se encuentra por debajo de la superficie de ajuste de las prótesis removibles parciales y totales. Esta patologÝa es mßs común en hombres que en mujeres y se observa mßs frecuentemente en sujetos con edades comprendidas entre 25 a 90 años. Diversos estudios han revelado que la E.S.P. estß asociada con la deteccion de especies de Cßndida y otros microorganismos, mientras que otros factores tales como trauma, ciertas enfermedades sistÚmicas y alteraciones del sistema inmune pueden estar involucrados, por lo que el hospedero responde mediante ciertos mecanismos ante la presencia de esta enfermedad. De allÝ que se han propuesto dos lÝneas de defensa ante la presencia de E.S.P. inducida por Cßndida; la primera de Ústas incluye entre otros: 1) factores fÝsicos, donde se destaca el papel que juegan las membranas mucosas bucales como barreras anatómicas; 2) compuestos salivales como la lisozima, lactoferrina e histatinas que inhiben el crecimiento de Cßndida y la interferencia de la microbiota bucal, la cual limita el sobrecrecimiento de este hongo, en tanto que la segunda destaca bßsicamente los mecanismos de inmunidad celular y humoral a nivel de los tejidos, haciendo Únfasis en la capacidad por parte de los polimorfonucleares y de los leucocitos eosinófilos de fagocitar a Cßndida, asÝ como el rol que juegan los anticuerpos como mecanismos de defensa contra esta enfermedad


Assuntos
Humanos , Masculino , Feminino , Candida albicans , Estomatite sob Prótese/etiologia , Estomatite sob Prótese/imunologia , Estomatite sob Prótese/microbiologia , Relações Hospital-Paciente , Interações Hospedeiro-Parasita , Prótese Total , Prótese Parcial Removível , Eosinófilos/fisiologia , Mucosa Bucal , Neutrófilos/fisiologia , Palato Mole , Saliva
17.
Oral Microbiol Immunol ; 17(5): 311-4, 2002 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-12354213

RESUMO

Cell-mediated immunity conferred by CD4+ T helper cells is considered the predominant host defense against mucosal Candida infections, with Thelper (Th1)-type responses associated with resistance to infection and Th2-type responses associated with susceptibility to infection. Oropharyngeal candidiasis, the most common oral opportunistic infection in HIV-infected persons, is associated with a Th2-type cytokine profile in saliva. To obtain more direct evidence for a role of salivary cytokines in susceptibility to oropharyngeal candidiasis during immunosuppression, we evaluated Th1/Th2-type cytokines in the saliva of those with denture stomatitis, a form of oropharyngeal candidiasis not related to immunosuppression. Results showed that HIV-negative denture wearers with and without denture stomatitis demonstrated a mixed Th1/Th2 cytokine profile with no significant differences found between the groups. These results suggest that a local Th cytokine dichotomy in saliva is not associated with susceptibility to denture stomatitis in immunocompetent persons.


Assuntos
Candidíase Bucal/imunologia , Citocinas/análise , Imunocompetência/imunologia , Saliva/imunologia , Estomatite sob Prótese/microbiologia , Linfócitos T Auxiliares-Indutores/imunologia , Adulto , Idoso , Candida albicans/isolamento & purificação , Prótese Total/microbiologia , Prótese Parcial/microbiologia , Suscetibilidade a Doenças/imunologia , Feminino , Soronegatividade para HIV , Humanos , Imunidade Inata/imunologia , Interferon gama/análise , Interleucina-10/análise , Interleucina-12/análise , Interleucina-2/análise , Interleucina-4/análise , Masculino , Pessoa de Meia-Idade , Proteínas e Peptídeos Salivares/análise , Estatísticas não Paramétricas , Estomatite sob Prótese/imunologia , Células Th1/imunologia , Células Th2/imunologia
18.
Folia Med (Plovdiv) ; 44(4): 39-44, 2002.
Artigo em Inglês | MEDLINE | ID: mdl-12751686

RESUMO

OBJECTIVE: Quantitative measurement of IgA and IgG antibodies in the serum and saliva of patients with denture stomatitis (stomatitis subprothetica) was the object of this study. METHODS: The indirect immunofluorescence technique (IFA) utilizing CIP 628 (serotype A) Candida albicans blastospores was employed. Anti-human goat and rabbit fluorescein isothiocyanate labeled serum was used. Serial serum dilutions from 1:20 to 1:1280 and saliva serial dilutions from 1:1 to 1:8 were prepared. Specimens were read using an ML-2 fluorescence microscope. Alternative analysis, analysis of variance and graphical analysis was used for statistical analysis of data. RESULTS: Anti-Candida antibodies (mainly IgG class) were detectable at dilutions of 1:40, 1:80 and 1:160 in the serum of patients with denture stomatitis compared with healthy controls. The saliva samples of these patients showed higher titers of anti-Candida antibodies compared with controls (1:8 for IgG and 1:4 for IgA). CONCLUSIONS: IgG anti-bodies were prevalent in the saliva of our patients and they were of diagnostic value. The IgA titers did not remain constant and could be a determinant of the disease course. Each patient showed an individual serologic profile. For this reason individualized interpretation of saliva antibodies as well as a comparison with the serum anti-Candida antibodies is recommended.


Assuntos
Anticorpos Antifúngicos/sangue , Candida/imunologia , Candidíase Bucal/diagnóstico , Saliva/imunologia , Estomatite sob Prótese/imunologia , Estomatite sob Prótese/microbiologia , Anticorpos Antifúngicos/análise , Técnica Indireta de Fluorescência para Anticorpo , Humanos , Imunoglobulina A/análise , Imunoglobulina A/sangue , Imunoglobulina G/análise , Imunoglobulina G/sangue
19.
Ned Tijdschr Tandheelkd ; 108(6): 223-8, 2001 Jun.
Artigo em Holandês | MEDLINE | ID: mdl-11441714

RESUMO

In general practice, the dentist can be confronted with a vesiculobullous lesion of the oral mucosa. In many cases the lesion can be classified as recurrent herpes labialis, but many other causes can induce a vesiculobullous lesion of the oral mucosa and perioral skin as well. This article gives an overview of the various vesiculous and bullous lesions of the oral mucous membranes. Special attention is given to the possible causes and their treatment.


Assuntos
Vesícula , Doenças da Boca , Mucosa Bucal/microbiologia , Estomatite/imunologia , Estomatite/virologia , Epidermólise Bolhosa/diagnóstico , Eritema Multiforme/diagnóstico , Doença de Mão, Pé e Boca/virologia , Herpangina/virologia , Herpes Labial/virologia , Herpes Zoster/virologia , Humanos , Impetigo/microbiologia , Líquen Plano Bucal/diagnóstico , Doenças da Boca/imunologia , Doenças da Boca/microbiologia , Doenças da Boca/virologia , Mucosa Bucal/imunologia , Mucosa Bucal/patologia , Mucosa Bucal/virologia , Penfigoide Mucomembranoso Benigno/imunologia , Pênfigo/imunologia , Estomatite/diagnóstico , Estomatite sob Prótese/imunologia , Estomatite Herpética/virologia
20.
Arch Immunol Ther Exp (Warsz) ; 48(2): 101-5, 2000.
Artigo em Inglês | MEDLINE | ID: mdl-10807050

RESUMO

Determinations of the blood serum levels of interleukin 6 (IL-6), tumor necrosis factor alpha (TNF-alpha) and their soluble receptors (sIL-6R, sTNFR) in denture stomatitis patients (DS) were performed. Serum levels of interleukins and their soluble receptors were measured using the ELISA method. In all examined patients mycological diagnostics were conducted using API 20C AUX stripe tests and an automatic ATB machine. Results were compared with those of healthy denture wearers (D), and controls (C). In DS patients, yeasts were isolated in 90.9%, in D in 66.7% of cases. The most often isolated species in both groups was Candida albicans. Mean concentrations of IL-6 and TNF-alpha were statistically significantly higher in DS and D groups compared to controls. Mean concentrations of sIL-6R were similar in all groups; however, concentrations of sTNFR in both DS and D groups were significantly lower compared to controls. There were no correlations found between values of IL-6 and TNF-alpha nor between examined interleukins and their soluble receptors.


Assuntos
Interleucina-6/sangue , Micoses/imunologia , Estomatite sob Prótese/imunologia , Fator de Necrose Tumoral alfa/metabolismo , Adulto , Candidíase Bucal/imunologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Receptores de Interleucina-6/sangue , Receptores do Fator de Necrose Tumoral/sangue
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