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1.
BMC Plant Biol ; 19(1): 367, 2019 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-31429697

RESUMO

BACKGROUND: Adaptation to abiotic stresses is crucial for the survival of perennial plants in a natural environment. However, very little is known about the underlying mechanisms. Here, we adopted a liquid culture system to investigate plant adaptation to repeated salt stress in Populus trees. RESULTS: We first evaluated phenotypic responses and found that plants exhibit better stress tolerance after pre-treatment of salt stress. Time-course RNA sequencing (RNA-seq) was then performed to profile changes in gene expression over 12 h of salt treatments. Analysis of differentially expressed genes (DEGs) indicated that significant transcriptional reprogramming and adaptation to repeated salt treatment occurred. Clustering analysis identified two modules of co-expressed genes that were potentially critical for repeated salt stress adaptation, and one key module for salt stress response in general. Gene Ontology (GO) enrichment analysis identified pathways including hormone signaling, cell wall biosynthesis and modification, negative regulation of growth, and epigenetic regulation to be highly enriched in these gene modules. CONCLUSIONS: This study illustrates phenotypic and transcriptional adaptation of Populus trees to salt stress, revealing novel gene modules which are potentially critical for responding and adapting to salt stress.


Assuntos
Adaptação Fisiológica/genética , Regulação da Expressão Gênica de Plantas , Populus/genética , Estresse Salino/genética , Transcrição Genética , Ontologia Genética , Redes Reguladoras de Genes , Genoma de Planta , Fenótipo , Populus/fisiologia , RNA de Plantas , Análise de Sequência de RNA , Transcriptoma , Árvores/genética , Árvores/fisiologia
2.
BMC Plant Biol ; 19(1): 370, 2019 Aug 22.
Artigo em Inglês | MEDLINE | ID: mdl-31438851

RESUMO

BACKGROUND: Accumulating evidences show that SPLs are crucial regulators of plant abiotic stress tolerance and the highly conserved module miR156/SPL appears to balance plant growth and stress responses. The halophyte Tamarix chinensis is highly resistant to salt tress. SPLs of T. chinensis (TcSPLs) and theirs roles in salt stress responses remain elusive. RESULTS: In this study, we conducted a systematic analysis of the TcSPLs gene family including 12 members belonging to 7 groups. The physicochemical properties and conserved motifs showed divergence among groups and similarity in each group. The microRNA response elements (MREs) are conserved in location and sequence, with the exception of first MRE within TcSPL5. The miR156-targeted SPLs are identified by dual-luciferase reporter assay of MRE-miR156 interaction. The digital expression gene profiles cluster suggested potential different functions of miR156-targeted SPLs vs non-targeted SPLs in response to salt stress. The expression patterns analysis of miR156-targeted SPLs with a reverse expression trend to TcmiR156 suggested 1 h (salt stress time) could be a critical time point of post-transcription regulation in salt stress responses. CONCLUSIONS: Our work demonstrated the post-transcription regulation of miR156-targeted TcSPLs and transcription regulation of non-targeted TcSPLs in salt stress responses, and would be helpful to expound the miR156/SPL-mediated molecular mechanisms underlying T. chinensis salt stress tolerance.


Assuntos
MicroRNAs/fisiologia , Proteínas de Plantas/fisiologia , RNA de Plantas/fisiologia , Estresse Salino/genética , Tamaricaceae/genética , Fatores de Transcrição/fisiologia , Motivos de Aminoácidos , Sequência Conservada , Genes de Plantas , Família Multigênica , Filogenia , Transcriptoma
3.
BMC Plant Biol ; 19(1): 353, 2019 Aug 14.
Artigo em Inglês | MEDLINE | ID: mdl-31412775

RESUMO

BACKGROUND: The PHOSPHATE1 (PHO1) gene family plays diverse roles in inorganic phosphate (Pi) transfer and signal transduction, and plant development. However, the functions and diversification of soybean PHO1 family are poorly understood. RESULTS: Cultivated soybean (Glycine max) was domesticated from wild soybean (Glycine soja). To illuminate their roles in this evolutionary process, we comparatively investigated the G. max PHO1 genes (GmPHO1) in Suinong 14 (SN14) and G. soja PHO1 genes (GsPHO1) in ZYD00006 (ZYD6). The sequences of the orthologous Gm-GsPHO1 pairs were grouped into two Classes. The expression of Class I in both SN14 and ZYD6 was widely but relatively high in developing fruits, whereas Class II was predominantly expressed in the roots. The whole family displayed diverse response patterns to salt stresses and Pi-starvation in roots. Between SN14 and ZYD6, most PHO1 genes responded similarly to salinity stresses, and half had sharp contrasts in response to Pi-starvation, which corroborated the differential response capacities to salinity and low-Pi stress between SN14 and ZYD6. Furthermore, in transgenic Arabidopsis plants, most Class II members and GmPHO1;H9 from Class I could enhance salt tolerance, while only two Class II genes (GmPHO1;H4 and GmPHO1;H8) differently altered sensitivity to Pi-starvation. The expression of critical genes was accordingly altered in either salt or Pi signaling pathways in transgenic Arabidopsis plants. CONCLUSIONS: Our work identifies some PHO1 genes as promising genetic materials for soybean improvement, and suggests that expression variation is decisive to functional divergence of the orthologous Gm-GsPHO1 pairs, which plays an adaptive role during soybean evolution.


Assuntos
Proteínas de Transporte de Fosfato/fisiologia , Proteínas de Plantas/fisiologia , Soja/genética , Adaptação Fisiológica , Arabidopsis/genética , Evolução Molecular , Proteínas de Transporte de Fosfato/genética , Proteínas de Transporte de Fosfato/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Plantas Geneticamente Modificadas , Estresse Salino/genética , Transdução de Sinais/genética , Soja/metabolismo
4.
Plant Sci ; 286: 28-36, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31300139

RESUMO

MYB family genes act as important regulators modulating the response to abiotic stress in plants. However, much less is known about MYB proteins in cotton. Here, we found that a cotton MYB gene, GhMYB73, was induced by NaCl and abscisic acid (ABA). Silencing GhMYB73 expression in cotton increased sensitivity to salt stress. The cotyledon greening rate of Arabidopsis thaliana over-expressing GhMYB73 under NaCl or mannitol treatment was significantly enhanced during the seedling germination stage. What's more, several osmotic stress-induced genes, such as AtNHX1, AtSOS3 and AtP5CS1, were more highly induced in the over-expression lines than in wild type under salt treatment, supporting the hypothesis that GhMYB73 contributes to salinity tolerance by improving osmotic stress resistance. Arabidopsis lines over-expressing GhMYB73 had superior germination and cotyledon greening under ABA treatment, and some abiotic stress-induced genes involved in ABA pathways (AtPYL8, AtABF3, AtRD29B and AtABI5), had increased transcription levels under salt-stress conditions in these lines. Furthermore, we found that GhMYB73 physically interacts with GhPYL8 and AtPYL8, suggesting that GhMYB73 regulates ABA signaling during salinity stress response. Taken together, over-expression of GhMYB73 significantly increases tolerance to salt and ABA stress, indicating that it can potentially be used in transgenic technology approaches to improve cotton salt tolerance.


Assuntos
Arabidopsis/metabolismo , Regulação da Expressão Gênica de Plantas/genética , Gossypium/fisiologia , Proteínas de Plantas/genética , Estresse Salino/genética , Fatores de Transcrição/genética , Arabidopsis/genética , Inativação Gênica , Genes myb , Gossypium/genética , Proteínas de Plantas/metabolismo , Plantas Geneticamente Modificadas/genética , Plantas Geneticamente Modificadas/metabolismo , Tolerância ao Sal/genética , Fatores de Transcrição/metabolismo
5.
BMC Genomics ; 20(1): 418, 2019 May 24.
Artigo em Inglês | MEDLINE | ID: mdl-31126236

RESUMO

BACKGROUND: To control the osmotic pressure in the body, physiological adjustments to salinity fluctuations require the fish to regulate body fluid homeostasis in relation to environmental change via osmoregulation. Previous studies related to osmoregulation were focused primarily on the gill; however, little is known about another organ involved in osmoregulation, the kidney. The salinity adaptation of marine fish involves complex physiological traits, metabolic pathways and molecular and gene networks in osmoregulatory organs. To further explore of the salinity adaptation of marine fish with regard to the role of the kidney, the euryhaline fish Scatophagus argus was employed in the present study. Renal expression profiles of S. argus at different salinity levels were characterized using RNA-sequencing, and an integrated approach of combining molecular tools with physiological and biochemical techniques was utilized to reveal renal osmoregulatory mechanisms in vivo and in vitro. RESULTS: S. argus renal transcriptomes from the hyposaline stress (0‰, freshwater [FW]), hypersaline stress (50‰, hypersaline water [HW]) and control groups (25‰) were compared to elucidate potential osmoregulatory mechanisms. In total, 19,012 and 36,253 differentially expressed genes (DEGs) were obtained from the FW and HW groups, respectively. Based on the functional classification of DEGs, the renal dopamine system-induced Na+ transport was demonstrated to play a fundamental role in osmoregulation. In addition, for the first time in fish, many candidate genes associated with the dopamine system were identified. Furthermore, changes in environmental salinity affected renal dopamine release/reuptake by regulating the expression of genes related to dopamine reuptake (dat and nkaα1), vesicular traffic-mediated dopamine release (pink1, lrrk2, ace and apn), DAT phosphorylation (CaMKIIα and pkcß) and internalization (akt1). The associated transcriptional regulation ensured appropriate extracellular dopamine abundance in the S. argus kidney, and fluctuations in extracellular dopamine produced a direct influence on Na+/K+-ATPase (NKA) expression and activity, which is associated with Na+ homeostasis. CONCLUSIONS: These transcriptomic data provided insight into the molecular basis of renal osmoregulation in S. argus. Significantly, the results of this study revealed the mechanism of renal dopamine system-induced Na+ transport is essential in fish osmoregulation.


Assuntos
Dopamina/metabolismo , Peixes/genética , Rim/metabolismo , Estresse Salino/genética , Sódio/metabolismo , Transcriptoma , Animais , Células Cultivadas , Peixes/metabolismo , Perfilação da Expressão Gênica , Homeostase , Transporte de Íons , Rim/enzimologia , Anotação de Sequência Molecular , Osmorregulação/genética , Potássio/metabolismo , Tolerância ao Sal , Análise de Sequência de RNA , ATPase Trocadora de Sódio-Potássio/metabolismo
6.
Cell Mol Biol (Noisy-le-grand) ; 65(3): 18-24, 2019 Mar 29.
Artigo em Inglês | MEDLINE | ID: mdl-30942152

RESUMO

Salinity is one of the most important abiotic stress factors that is expanding its influence because of global climate change and global warming. It causes gene expression changes, a reduction in seed germination and related characteristics, and poor seedling establishment in many crop plants by creating a lower osmotic potential in the seedbed and/or toxic ion effects in germinated seeds. In recent years, seed priming has been considered a promising strategy in modern stress management to protect plants against stress conditions. This study was conducted to elucidate the effects of osmopriming with polyethylene glycol 6000 (PEG-6000) on seed germination, seedling growth and gene expression in the common vetch (Vicia sativa L.) in different saline conditions. Common vetch seeds were primed with PEG-6000 solutions having different osmotic potentials (0.00, -0.50,  -0.75, -1.00, -1.25, and -1.50 MPa) for 12 hours. Control (un-primed) and primed seeds were germinated and seedlings were grown in different saline conditions (EC= zero, 4, 8 and 16 dS m-1). Furthermore, gene expression was compared in the primed seedlings in two different osmotic potentials (0.00 and -1.50 MPa) by microarray technology. Results demonstrated that germination percentage of common vetch seeds and seedling growth were diminished by high salinity. However, several priming treatments alleviated the adverse effects of high salinity on germination and early seedling growth of common vetch. The microarray showed that the expression of many genes in both stress and normal conditions was not significantly different.


Assuntos
Regulação da Expressão Gênica de Plantas , Germinação , Salinidade , Estresse Salino , Plântula/crescimento & desenvolvimento , Sementes/crescimento & desenvolvimento , Vicia sativa/genética , Vicia sativa/fisiologia , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Germinação/efeitos dos fármacos , Germinação/genética , Raízes de Plantas/efeitos dos fármacos , Raízes de Plantas/metabolismo , Polietilenoglicóis/farmacologia , Estresse Salino/efeitos dos fármacos , Estresse Salino/genética , Plântula/efeitos dos fármacos , Plântula/genética , Sementes/efeitos dos fármacos , Sementes/genética , Vicia sativa/efeitos dos fármacos
7.
BMC Plant Biol ; 19(1): 164, 2019 Apr 27.
Artigo em Inglês | MEDLINE | ID: mdl-31029105

RESUMO

BACKGROUND: Circular RNAs (circRNAs) are 3'-5' head-to-tail covalently closed non-coding RNA that have been proved to play essential roles in many cellular and developmental processes. However, no information relate to cucumber circRNAs is available currently, especially under salt stress condition. RESULTS: In this study, we sequenced circRNAs in cucumber and a total of 2787 were identified, with 1934 in root and 44 in leaf being differentially regulated under salt stress. Characteristics analysis of these circRNAs revealed following features: most of them are exon circRNAs (79.51%) and they prefer to arise from middle exon(s) of parent genes (2035/2516); moreover, most of circularization events (88.3%) use non-canonical-GT/AG splicing signals; last but not least, pairing-driven circularization is not the major way to generate cucumber circRNAs since very few circRNAs (18) contain sufficient flanking complementary sequences. Annotation and enrichment analysis of both parental genes and target mRNAs were launched to uncover the functions of differentially expressed circRNAs induced by salt stress. The results showed that circRNAs may be paly roles in salt stress response by mediating transcription, signal transcription, cell cycle, metabolism adaptation, and ion homeostasis related pathways. Moreover, circRNAs may function to regulate proline metabolisms through regulating associated biosynthesis and degradation genes. CONCLUSIONS: The present study identified large number of cucumber circRNAs and function annotation revealed their possible biological roles in response to salt stress. Our findings will lay a solid foundation for further structure and function studies of cucumber circRNAs.


Assuntos
Cucumis sativus/genética , Cucumis sativus/fisiologia , RNA de Plantas/genética , RNA/genética , Estresse Salino/genética , Sequência de Bases , Biomassa , Cucumis sativus/crescimento & desenvolvimento , Éxons/genética , Regulação da Expressão Gênica de Plantas , Ontologia Genética , Redes Reguladoras de Genes , Genes de Plantas , Transporte de Íons , MicroRNAs/genética , MicroRNAs/metabolismo , Anotação de Sequência Molecular , Raízes de Plantas/genética , Raízes de Plantas/fisiologia , RNA/metabolismo , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , RNA de Plantas/metabolismo
8.
Plant Physiol Biochem ; 139: 478-484, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31005823

RESUMO

Salinity is a common environmental challenge limiting worldwide agricultural crop yield. Plants employ epigenetic regulatory strategies, such as DNA methylation which relatively allows rapid adaptation to new conditions in response to environmental stresses. Brassinosteroids (BRs) are a novel group of phytohormones recognized as transcription and translation regulators which are able to mitigate the impact of environmental stresses on the plants. In the current investigation, the influence of salinity and 24-epibrassinolide (24-epiBL) was investigated on the extent and pattern of cytosine DNA methylation using methylation-sensitive amplified polymorphisms (MSAP) technique in flax. Upon NaCl (150 mM) exposure, total methylation of CCGG sequences was decreased in comparison to control plants, while 24-epiBL (10-8 M) induced total methylation under salinity stress. Sequencing and analysis of six randomly selected MSAP fragments detected genes involved in various biological and molecular processes such as vitamine B1 biosynthesis, protein targeting and localization, post-translational modification and gene regulation. In conclusion, 24-epiBL seed priming could play critical role in regulation of cellular and biological processes in response to salt stress by epigenetic modification and induction of methylation.


Assuntos
Brassinosteroides/farmacologia , Citosina/química , Metilação de DNA/efeitos dos fármacos , Metilação de DNA/genética , Linho/efeitos dos fármacos , Linho/genética , Epigênese Genética/efeitos dos fármacos , Epigênese Genética/genética , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Regulação da Expressão Gênica de Plantas/genética , Estresse Salino/efeitos dos fármacos , Estresse Salino/genética
9.
BMC Mol Biol ; 20(1): 11, 2019 04 08.
Artigo em Inglês | MEDLINE | ID: mdl-30961536

RESUMO

BACKGROUND: Camels possess the characteristics of salt- and drought-resistances, due to the long-time adaption to the living environment in desert. The camel resistance research on transcriptome is rare and deficient, especially reabsorption in renal cortex. Non-coding RNAs are normally considered as the RNA molecules that are not translated into proteins, their current roles remain mostly in regulation of information flux from DNA to protein, further on normal life activities and diseases. In order to reveal the mysterious veil of the post-transcriptional regulation of ncRNAs in renal cortex for the first time as far as we know, we designed and carried out the experiment of salt stress and water-deprivation stress in camel. RESULTS: By means of RNA-seq in renal cortex of Alxa Bactrian Camel (Camelus bactrianus), we identified certain significantly differential RNAs, including 4 novel lncRNAs, 11 miRNAs and 13 mRNAs under salt stress, 0 lncRNAs, 18 miRNAs and 14 mRNAs under water-deprivation stress. By data analysis, the response pathway of post-transcriptional regulation concerning salt and water-deprivation stresses was put forward, involving preventing sodium from entering the cell, purifying of water and compensating neutral amino acids by miR-193b, miR-542-5p interaction with SLC6A19 mRNA. CONCLUSION: Based on the resistance-related lncRNAs, miRNAs, and mRNAs, we proposed the post-transcriptional regulation pathway to explain how camels respond to salt and water-deprivation stresses in the ncRNAs regulation level of renal cortex for the first time, thus hoping to provide a theoretical basis for therapy of disease that is similar to high blood pressure in humans.


Assuntos
Camelus/genética , Camelus/fisiologia , Córtex Renal/fisiologia , RNA não Traduzido/genética , Estresse Salino/genética , Transcriptoma , Privação de Água , Sistemas de Transporte de Aminoácidos Neutros/genética , Animais , Regulação da Expressão Gênica , MicroRNAs/genética , Processamento Pós-Transcricional do RNA , RNA Longo não Codificante/genética , RNA Mensageiro/genética
10.
Ecotoxicol Environ Saf ; 174: 245-254, 2019 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-30831473

RESUMO

Salinity decreases the yield and quality of crops. Silicon (Si) has been widely reported to have beneficial effects on plant growth and development under salt stress. However, the mechanism is still poorly understood. In an attempt to identify genes or gene networks that may be orchestrated to improve salt tolerance of cucumber plants, we sequenced the transcriptomes of both control and salt-stressed cucumber leaves in the presence or absence of added Si. Seedlings of cucumber 'JinYou 1' were subjected to salt stress (75 mM NaCl) without or with addition of 0.3 mM Si. Plant growth, photosynthetic gas exchange and transcriptomic dynamics were investigated. The results showed that Si addition improved the growth and photosynthetic performance of cucumber seedlings under salt stress. The comparative transcriptome analysis revealed that Si played an important role in shaping the transcriptome of cucumber: the expressions of 1469 genes were altered in response to Si treatment in the control conditions, and these genes were mainly involved in ion transport, hormone and signal transduction, biosynthetic and metabolic processes, and stress and defense responses. Under salt stress alone, 1482 genes with putative functions associated with metabolic processes and responses to environmental stimuli have changed their expression levels. Si treatment shifted the transcriptome of salt-stressed cucumber back to that of the control, as evidenced that among the 708 and 774 genes that were up- or down-regulated under salt stress, a large majority of them (609 and 595, respectively) were reverted to the normal expression levels. These results suggest that Si may act as an elicitor to precondition cucumber plants and induce salt tolerance. The study may help us understand the mechanism for silicon-mediated salt tolerance and provide a theoretical basis for silicon application in crop production in saline soils.


Assuntos
Cucumis sativus/efeitos dos fármacos , Estresse Salino/efeitos dos fármacos , Silicatos/farmacologia , Transcriptoma/efeitos dos fármacos , Cucumis sativus/genética , Cucumis sativus/crescimento & desenvolvimento , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Modelos Teóricos , Fotossíntese/efeitos dos fármacos , Fotossíntese/genética , Desenvolvimento Vegetal/efeitos dos fármacos , Desenvolvimento Vegetal/genética , Salinidade , Estresse Salino/genética , Tolerância ao Sal/efeitos dos fármacos , Tolerância ao Sal/genética , Cloreto de Sódio/administração & dosagem
11.
Int J Mol Sci ; 20(6)2019 Mar 13.
Artigo em Inglês | MEDLINE | ID: mdl-30871161

RESUMO

Soil salinization is a major environmental stresses that seriously threatens land use efficiency and crop yields worldwide. Although the overall response of plants to NaCl has been well studied, the contribution of protein phosphorylation to the detoxification and tolerance of NaCl in okra (Abelmoschus esculentus L.) seedlings is unclear. The molecular bases of okra seedlings' responses to 300 mM NaCl stress are discussed in this study. Using a combination of affinity enrichment, tandem mass tag (TMT) labeling and high-performance liquid chromatography⁻tandem mass spectrometry analysis, a large-scale phosphoproteome analysis was performed in okra. A total of 4341 phosphorylation sites were identified on 2550 proteins, of which 3453 sites of 2268 proteins provided quantitative information. We found that 91 sites were upregulated and 307 sites were downregulated in the NaCl/control comparison group. Subsequently, we performed a systematic bioinformatics analysis including gene ontology annotation, domain annotation, subcellular localization, and Kyoto Encyclopedia of Genes and Genomes pathway annotation. The latter revealed that the differentially expressed proteins were most strongly associated with 'photosynthesis antenna proteins' and 'RNA degradation'. These differentially expressed proteins probably play important roles in salt stress responses in okra. The results should help to increase our understanding of the molecular mechanisms of plant post-translational modifications in response to salt stress.


Assuntos
Abelmoschus/metabolismo , Fosforilação/fisiologia , Proteoma/metabolismo , Estresse Salino/fisiologia , Plântula/metabolismo , Abelmoschus/genética , Biologia Computacional/métodos , Regulação para Baixo/genética , Regulação para Baixo/fisiologia , Ontologia Genética , Genes de Plantas/genética , Genes de Plantas/fisiologia , Genoma de Planta/genética , Genoma de Planta/fisiologia , Fosforilação/genética , Processamento de Proteína Pós-Traducional/genética , Processamento de Proteína Pós-Traducional/fisiologia , Proteoma/genética , Proteômica/métodos , Estresse Salino/genética , Plântula/genética , Regulação para Cima/genética , Regulação para Cima/fisiologia
12.
Genes Genomics ; 41(5): 599-612, 2019 05.
Artigo em Inglês | MEDLINE | ID: mdl-30840180

RESUMO

BACKGROUND: Sinonovacula constricta is an economically important bivalve species in China, Korea and Japan that widely resides in estuarine and coastal areas where salinity fluctuates rapidly. However, little is known about its adaptation mechanisms to acute salt stresses. OBJECTIVE: To reveal the underlying molecular mechanisms involved in acute salt stresses in juvenile S. constricta. METHODS: Nine cDNA libraries (triplicate each trial) were established from juvenile S. constricta, which were subjected to low salinity (5 psu), optimal salinity (15 psu) and high salinity (25 psu) for 6 h, respectively. RESULTS: Illumina sequencing generated 478,587,310 clean reads totally, which were assembled into 427,057 transcripts of 246,672 unigenes. Compared with the control, 1259 and 2163 differentially expressed genes (DEGs) were identified under acute low and high salt stresses, respectively. GO and KEGG enrichment analyses of DEGs revealed that several key metabolic modulations were mainly responsible for the acute salt stresses. According to the significantly highlighted KEGG pathways, some key DEGs were identified and discussed in details. Notably, based on which, some potential osmolytes were further speculated. CONCLUSION: Here, we carried out a unique report of comparative transcriptome analyses in juvenile S. constricta in response to acute salt stresses. The identified DEGs and their significantly enriched GO terms and KEGG pathways were critical for understanding and further investigating the underlying the physical and biochemical performances, and ultimately facilitated S. constricta breeding. Besides, the transcriptome data greatly enriched the genetic information of S. constricta, which were valuable for promoting its molecular biology researches.


Assuntos
Bivalves/genética , Perfilação da Expressão Gênica/métodos , Estresse Salino/genética , Adaptação Biológica/genética , Animais , Bivalves/fisiologia , China , Japão , Anotação de Sequência Molecular/métodos , República da Coreia , Tolerância ao Sal/genética , Tolerância ao Sal/fisiologia
13.
Plant Sci ; 280: 132-142, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30823991

RESUMO

High salinity severely inhibits the growth and productivity of grape plants. However, knowledge of salt-stress regulation remains limited in WRKY members of grapes. Here, we isolated a novel VvWRKY30 gene from Vitis vinifera L. and studied its role in salt-stress resistance. The VvWRKY30 protein fused with green fluorescent protein localized to the nucleus and the transcriptional activation activity of VvWRKY30 was confirmed in yeast. Moreover, VvWRKY30 showed key transcriptional activity domain at the N-terminal and specifically binds to the W-BOX. VvWRKY30 showed the highest expression in the shoot tip and functional leaves of grape plants. VvWRKY30 expression was induced by salt as well as stress signaling molecules H2S and H2O2. Overexpression of VvWRKY30 in Arabidopsis increased its resistance to salt stress at different stages of growth. Under salinity stress, VvWRKY30 overexpressing lines had higher antioxidant activities and lower reactive oxygen species contents. Soluble sugar and proline concentrations also increased in VvWRKY30 overexpressing lines in the presence of NaCl. In addition, the transcription of genes related to antioxidant biosynthesis, glyco-metabolism and proline biosynthesis increased in the VvWRKY30 overexpressing lines. Taken together, this study confirmed the important role of VvWRKY30 in increasing salt stress resistance by regulating reactive oxygen species-scavenging and the accumulation of osmoticum.


Assuntos
Fatores de Transcrição/metabolismo , Vitis/metabolismo , Regulação da Expressão Gênica de Plantas , Peróxido de Hidrogênio/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Estresse Salino/genética , Estresse Salino/fisiologia , Fatores de Transcrição/genética , Vitis/genética
14.
Plant Sci ; 281: 213-222, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30824054

RESUMO

Eukaryotic nucleases are involved in processes such as DNA restriction digestion, repair, recombination, transposition, and programmed cell death (PCD). Studies on the role of nucleases have mostly focused on PCD during plant development, while the information on nucleases involved in responses to different abiotic stress conditions remains limited. Here, we identified a Ca2+-dependent nuclease, AtCaN2, in Arabidopsis thaliana and characterized its activity, expression patterns, and involvement in plant responses to salt stress. AtCaN2 showed a dual endonuclease and exonuclease activity, being able to degrade circular plasmids, RNA, single-stranded DNA, and double-stranded DNA. Expression analysis showed that AtCaN2 was strongly induced in senescent siliques and by salt stress. Overexpression of AtCaN2 decreased the plant tolerance to salt stress conditions, leading to an excessive H2O2 accumulation. However, an atcan2 mutant showed better tolerance to salt stress and a lower level of H2O2 accumulation. Moreover, the expression of several genes (AtAPX1, AtGPX8, and AtSOD1), encoding reactive oxygen species-scavenging enzymes (ascorbate peroxidase 1, glutathione peroxidase 8, and superoxide dismutase 1, respectively), was highly induced in the atcan2 mutant under salt stress conditions. In addition, salt-stress-induced cell death was increased in the AtCaN2-overexpressing transgenic plant but decreased in the atcan2 mutant. On the basis of these findings, we conclude that AtCaN2 plays a negative role in plant tolerance to salt stress. A AtCaN2 knock out could reduce ROS accumulation, decrease ROS-induced PCD, and improve overall plant tolerance.


Assuntos
Proteínas de Arabidopsis/metabolismo , Arabidopsis/enzimologia , Arabidopsis/metabolismo , Arabidopsis/efeitos dos fármacos , Proteínas de Arabidopsis/genética , Endonucleases/genética , Endonucleases/metabolismo , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Regulação da Expressão Gênica de Plantas/genética , Peróxido de Hidrogênio/metabolismo , Plantas Geneticamente Modificadas/efeitos dos fármacos , Plantas Geneticamente Modificadas/enzimologia , Plantas Geneticamente Modificadas/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Estresse Salino/genética
15.
Int J Mol Sci ; 20(4)2019 Feb 14.
Artigo em Inglês | MEDLINE | ID: mdl-30769802

RESUMO

Sodium chloride (NaCl) induced expression of a jacalin-related mannose-binding lectin (JRL) gene in leaves, roots, and callus cultures of Populus euphratica (salt-resistant poplar). To explore the mechanism of the PeJRL in salinity tolerance, the full length of PeJRL was cloned from P. euphratica and was transformed into Arabidopsis. PeJRL was localized to the cytoplasm in mesophyll cells. Overexpression of PeJRL in Arabidopsis significantly improved the salt tolerance of transgenic plants, in terms of seed germination, root growth, and electrolyte leakage during seedling establishment. Under NaCl stress, transgenic plants retained K⁺ and limited the accumulation of Na⁺. PeJRL-transgenic lines increased Na⁺ extrusion, which was associated with the upward regulation of SOS1, AHA1, and AHA2 genes encoding plasma membrane Na⁺/proton (H⁺) antiporter and H⁺-pumps. The activated H⁺-ATPases in PeJRL-overexpressed plants restricted the channel-mediated loss of K⁺ that was activated by NaCl-induced depolarization. Under salt stress, PeJRL⁻transgenic Arabidopsis maintained reactive oxygen species (ROS) homeostasis by activating the antioxidant enzymes and reducing the production of O2- through downregulation of NADPH oxidases. Of note, the PeJRL-transgenic Arabidopsis repressed abscisic acid (ABA) biosynthesis, thus reducing the ABA-elicited ROS production and the oxidative damage during the period of salt stress. A schematic model was proposed to show the mediation of PeJRL on ABA response, and ionic and ROS homeostasis under NaCl stress.


Assuntos
Arabidopsis/genética , Lectinas de Ligação a Manose/genética , Plantas Geneticamente Modificadas/genética , Estresse Salino/genética , Arabidopsis/efeitos dos fármacos , Arabidopsis/crescimento & desenvolvimento , Citoplasma/efeitos dos fármacos , Citoplasma/genética , Regulação da Expressão Gênica de Plantas , Homeostase , Lectinas de Ligação a Manose/química , Folhas de Planta/efeitos dos fármacos , Folhas de Planta/genética , Lectinas de Plantas/genética , Raízes de Plantas/efeitos dos fármacos , Raízes de Plantas/genética , Populus , Espécies Reativas de Oxigênio/química , Tolerância ao Sal/genética , Cloreto de Sódio/efeitos adversos
16.
Plant Physiol Biochem ; 137: 14-24, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30710795

RESUMO

Glutamyl-tRNA reductase1 (HEMA1) and ferrochelatase1 (FC1) are both expressed in response to salt stress in the biosynthetic pathway of tetrapyrroles. Peanut (Arachis hypogaea L.) HEMA1 and FC1 were isolated by RT-PCR. The amino acid sequence encoded by the two genes showed high similarity with that in other plant species. The AhFC1 fusion protein was verified to function in chloroplast using Arabidopsis mesophyll protoplast. Sense and wild-type (WT) tobaccos were used to further study the physiological effects of AhHEMA1 and AhFC1. Compared with WT, the Heme contents and germination rate were higher in AhFC1 overexpressing plants under salt stress. Meanwhile, overexpressing AhHEMA1 also led to higher ALA and chlorophyll contents and multiple physiological changes under salt stress, such as higher activities of superoxide dismutase (SOD) and ascorbate peroxidase (APX), lower contents of reactive oxygen species (ROS) and slighter membrane damage. In addition, the activities of CAT, POD and APX in the AhFC1 overexpressing plants were significantly higher than that in WT lines under salt stress, but the activity of SOD between the WT plants and the transgenic plants did not exhibit significant differences. These results suggested that, peanut can enhance resistance to salt stress by improving the biosynthesis of tetrapyrrole biosynthetic.


Assuntos
Arachis/genética , Proteínas de Plantas/genética , Estresse Salino/genética , Tabaco/genética , Ácido Aminolevulínico/metabolismo , Membrana Celular/metabolismo , Clorofila/genética , Clorofila/metabolismo , Cloroplastos/genética , Cloroplastos/metabolismo , Enzimas/genética , Enzimas/metabolismo , Regulação da Expressão Gênica de Plantas , Germinação/genética , Heme/biossíntese , Heme/genética , Proteínas de Plantas/metabolismo , Plantas Geneticamente Modificadas , Espécies Reativas de Oxigênio/metabolismo , Estresse Salino/fisiologia , Plântula/genética , Plântula/metabolismo , Tetrapirróis/genética , Tetrapirróis/metabolismo , Tabaco/fisiologia
17.
Plant Physiol Biochem ; 137: 179-188, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30798172

RESUMO

MYB proteins are major transcription factors that play significant roles in plant defenses against various stresses. However, available information regarding stress-related MYB genes in maize is minimal. Herein, a maize MYB gene, ZmMYB3R, was cloned and functionally characterized. Subcellular localisation analysis showed that ZmMYB3R is localised to the nucleus. Yeast one-hybrid results revealed that ZmMYB3R has trans-activation activity, and a minimal activation domain at the C-terminus spanning residues 217-563. Gene expression analysis suggested that ZmMYB3R was induced by drought, salt and abscisic acid (ABA). Transgenic Arabidopsis plants overexpressing ZmMYB3R displayed enhanced growth performance and higher survival rates, elevated catalase (CAT), peroxidase (POD) and superoxide dismutase (SOD) enzyme activities, increased sensitivity to ABA, and regulation of the stomatal aperture, suggesting that ZmMYB3R enhances tolerance to drought and salt stress. qRT-PCR assays revealed elevated expression levels of stress/ABA genes in transgenic plants following stress treatments. Moreover, transgenic plants accumulated higher ABA content than wild-type plants under drought and salt stress conditions. Collectively, these results indicate that ZmMYB3R is a positive transcription factor that enhances tolerance to drought and salt stress via an ABA-dependent pathway. The findings may prove useful for engineering economically important crops.


Assuntos
Arabidopsis/genética , Proteínas de Plantas/genética , Estresse Salino/genética , Fatores de Transcrição/genética , Zea mays/genética , Ácido Abscísico/metabolismo , Ácido Abscísico/farmacologia , Arabidopsis/efeitos dos fármacos , Arabidopsis/fisiologia , Núcleo Celular/metabolismo , Secas , Enzimas/metabolismo , Regulação da Expressão Gênica de Plantas , Filogenia , Proteínas de Plantas/metabolismo , Plantas Geneticamente Modificadas , Estresse Salino/fisiologia , Tabaco , Fatores de Transcrição/metabolismo
18.
BMC Plant Biol ; 19(1): 66, 2019 Feb 11.
Artigo em Inglês | MEDLINE | ID: mdl-30744558

RESUMO

BACKGROUND: Soil salinity is one of the major serious factors that affect agricultural productivity of almost all crops worldwide, including the important oilseed crop sesame. In order to improve salinity resistance in sesame, it is crucial to understand the molecular mechanisms underlying the adaptive response to salinity stress. RESULTS: In the present study, two contrasting sesame genotypes differing in salt tolerance were used to decipher the adaptive responses to salt stress based on morphological, transcriptome and metabolome characterizations. Morphological results indicated that under salt stress, the salt-tolerant (ST) genotype has enhanced capacity to withstand salinity stress, higher seed germination rate and plant survival rate, as well as better growth rate than the salt-sensitive genotype. Transcriptome analysis revealed strongly induced salt-responsive genes in sesame mainly related to amino acid metabolism, carbohydrate metabolism, biosynthesis of secondary metabolites, plant hormone signal transduction, and oxidation-reduction process. Especially, several pathways were preferably enriched with differentially expressed genes in ST genotype, including alanine, aspartate and glutamate metabolism, carotenoid biosynthesis, galactose metabolism, glycolysis/gluconeogenesis, glyoxylate and dicarboxylate metabolism, porphyrin and chlorophyll metabolism. Metabolome profiling under salt stress showed a higher accumulation degree of metabolites involved in stress tolerance in ST, and further highlighted that the amino acid metabolism, and sucrose and raffinose family oligosaccharides metabolism were enhanced in ST. CONCLUSIONS: These findings suggest that the candidate genes and metabolites involved in crucial biological pathways may regulate salt tolerance of sesame, and increase our understanding of the molecular mechanisms underlying the adaptation of sesame to salt stress.


Assuntos
Metaboloma/genética , Transcriptoma/genética , Regulação da Expressão Gênica de Plantas , Genótipo , Rafinose/metabolismo , Estresse Salino/genética , Estresse Salino/fisiologia , Tolerância ao Sal/genética , Tolerância ao Sal/fisiologia
19.
Sci Total Environ ; 662: 168-179, 2019 Apr 20.
Artigo em Inglês | MEDLINE | ID: mdl-30690352

RESUMO

Intertidal macroalgae are highly resistant to hypersaline stress conditions. However, the underlying mechanism remains unknown. In the present study, the mechanism behind Pyropia haitanensis responses to two hypersaline stress conditions [100‰ (HSS_100) and 110‰ (HSS_110)] was investigated via analyses of physiological and transcriptomic changes. We observed that the differences between the responses of Py. haitanensis to HSS_100 and HSS_110 conditions involved the following three aspects: osmotic regulation, ionic homeostasis, and adjustment to secondary stresses. First, the water retention of Py. haitanensis was maintained through increased expansin production under HSS_100 conditions, while cell wall pectin needed to be protected from hydrolysis via the increased abundance of a pectin methylesterase inhibitor under HSS_110 conditions. Meanwhile, Py. haitanensis achieved stable and rapid osmotic adjustments because of the coordinated accumulation of inorganic ions (K+, Na+, and Cl-) and organic osmolytes (glycine betaine and trehalose) under HSS_100 conditions, but not under HSS_110 conditions. Second, Py. haitanensis maintained a higher K+/Na+ ratio under HSS_100 conditions than under HSS_110 conditions, mainly via the export of Na+ into the apoplast rather than compartmentalizing it into the vacuoles, and the enhanced uptake and retention of K+. However, K+/Na+ homeostasis was not completely disrupted during a short-term exposure to HSS_110 conditions. Finally, the Py. haitanensis antioxidant system scavenged more ROS and synthesized more heat shock proteins under HSS_100 conditions than under HSS_110 conditions, although thalli may have been able to maintain a certain redox balance during a short-term exposure to HSS_110 conditions. These differences may explain why Py. haitanensis can adapt to HSS_100 conditions rather than HSS_110 conditions, and also why the thalli exposed to HSS_110 conditions can recover after being transferred to normal seawater. Thus, the data presented herein may elucidate the mechanisms enabling Pyropia species to tolerate the sudden and periodic changes in salinity typical of intertidal systems.


Assuntos
Proteínas de Algas/genética , Homeostase/genética , Rodófitas/fisiologia , Estresse Salino/genética , Transcriptoma , Aclimatação , Proteínas de Algas/metabolismo , Perfilação da Expressão Gênica , Estudo de Associação Genômica Ampla , Sequenciamento de Nucleotídeos em Larga Escala , Rodófitas/genética , Água do Mar/análise , Alga Marinha/genética , Alga Marinha/fisiologia
20.
BMC Genomics ; 20(1): 51, 2019 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-30651065

RESUMO

BACKGROUND: Plant basic leucine zipper (bZIP) transcription factors play crucial roles in plant growth, development, and abiotic stress responses. However, systematic investigation and analyses of the bZIP gene family in peanut are lacking in spite of the availability of the peanut genome sequence. RESULTS: In this study, we identified 50 and 45 bZIP genes from Arachis duranensis and A. ipaensis genomes, respectively. Phylogenetic analysis showed that Arachis bZIP genes were classified into nine groups, and these clusters were supported by several group-specific features, including exon/intron structure, intron phases, MEME motifs, and predicted binding site structure. We also identified possible variations in DNA-binding-site specificity and dimerization properties among different Arachis bZIPs by inspecting the amino acid residues at some key sites. Our analysis of the evolutionary history analysis indicated that segmental duplication, rather than tandem duplication, contributed greatly to the expansion of this gene family, and that most Arachis bZIPs underwent strong purifying selection. Through RNA-seq and quantitative real-time PCR (qRT-PCR) analyses, the co-expressed, differentially expressed and several well-studied homologous bZIPs were identified during seed development stages in peanut. We also used qRT-PCR to explore changes in bZIP gene expression in response to salt-treatment, and many candidate bZIPs in groups A, B, and S were proven to be associated with the salt-stress response. CONCLUSIONS: This study have conducted a genome-wide identification, characterization and expression analysis of bZIP genes in Arachis genomes. Our results provide insights into the evolutionary history of the bZIP gene family in peanut and the funcntion of Arachis bZIP genes during seed development and in response to salt stress.


Assuntos
Arachis/genética , Fatores de Transcrição de Zíper de Leucina Básica/metabolismo , Perfilação da Expressão Gênica , Regulação da Expressão Gênica de Plantas , Genoma de Planta , Estresse Salino/genética , Sementes/crescimento & desenvolvimento , Sementes/genética , Arachis/fisiologia , Fatores de Transcrição de Zíper de Leucina Básica/genética , Sítios de Ligação , Duplicação Gênica , Regulação da Expressão Gênica no Desenvolvimento , Genes de Plantas , Íntrons/genética , Família Multigênica , Filogenia , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Multimerização Proteica
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