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1.
J Cosmet Dermatol ; 17(6): 1008-1015, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-29316152

RESUMO

BACKGROUND: Striae distensae are dermal scars with a linear atrophic depression. The exact origin of striae distensae remains unrevealed, but low expression of collagen and fibronectin genes in the affected tissue was found. Several treatment modalities have been proposed, yet no consistent modality is available. AIM OF THE WORK: To evaluate and compare the efficacy and safety of carboxytherapy vs platelet-rich plasma (PRP) in treatment of stretch marks. PATIENTS AND METHODS: This study included 20 patients with striae alba. Every patient received treatment in the form of PRP injection in their right side (group A) and carboxytherapy session in their left side (group B) every 3-4 weeks for 4 sessions. Skin biopsies were taken before and after treatment, and they were subjected to fibronectin immunohistochemical stain. RESULTS: There was a significant improvement in striae alba in both groups after than before treatment. There was no significant difference between both groups as regards either percentage of improvement, response (grading scale), or patient satisfaction. The fibronectin-stained area was significantly higher in both groups after than before treatment, and it was significantly higher after treatment in group (B) than group (A). CONCLUSIONS: Both methods were safe and effective with minimal side effects. There was no significant difference between both methods of treatments. This was confirmed histopathologically by fibronectin expression which is found to be low in striae and increased significantly after treatment. But fibronectin expression was higher in group (B) than (A).


Assuntos
Dióxido de Carbono/uso terapêutico , Plasma Rico em Plaquetas , Estrias de Distensão/patologia , Estrias de Distensão/terapia , Adolescente , Adulto , Biópsia , Dióxido de Carbono/administração & dosagem , Dióxido de Carbono/efeitos adversos , Feminino , Fibronectinas/metabolismo , Humanos , Imuno-Histoquímica , Injeções Intradérmicas , Masculino , Satisfação do Paciente , Pele/patologia , Estrias de Distensão/metabolismo , Resultado do Tratamento , Adulto Jovem
2.
Lasers Med Sci ; 33(3): 609-617, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29308552

RESUMO

This research work mainly deals with studying qualitatively the changes in the dermal collagen of two forms of striae distensae (SD) namely striae rubrae (SR) and striae albae (SA) when compared to normal skin (NS) using confocal Raman spectroscopy. The methodology includes an in vivo human skin study for the comparison of confocal Raman spectra of dermis region of SR, SA, and NS by supervised multivariate analysis using partial least squares discriminant analysis (PLS-DA) to determine qualitatively the changes in dermal collagen. These groups are further analyzed for the extent of hydration of dermal collagen by studying the changes in the water content bound to it. PLS-DA score plot showed good separation of the confocal Raman spectra of dermis region into SR, SA, and NS data groups. Further analysis using loading plot and S-plot indicated the participation of various components of dermal collagen in the separation of these groups. Bound water content analysis showed that the extent of hydration of collagen is more in SD when compared to NS. Based on the results obtained, this study confirms the active involvement of dermal collagen in the formation of SD. It also emphasizes the need to study quantitatively the role of these various biochemical changes in the dermal collagen responsible for the variance between SR, SA, and NS.


Assuntos
Colágeno/metabolismo , Derme/metabolismo , Análise Espectral Raman/métodos , Estrias de Distensão/diagnóstico , Estrias de Distensão/metabolismo , Adulto , Análise Discriminante , Feminino , Humanos , Análise dos Mínimos Quadrados , Água/metabolismo
3.
Br J Dermatol ; 178(3): 749-760, 2018 03.
Artigo em Inglês | MEDLINE | ID: mdl-28815554

RESUMO

BACKGROUND: Striae gravidarum (SG), or stretch marks of pregnancy, begin as erythematous streaks and mature into hypopigmented atrophic bands. OBJECTIVES: In order to investigate molecular alterations that may promote atrophy of SG, we investigated dermal type I collagen fibrils, which provide human skin with support. METHODS: We obtained skin samples of recently developed, erythematous abdominal SG from pregnant women. To examine the organization of collagen fibrils, second-harmonic generation imaging was performed using multiphoton microscopy. Immunostaining was used to determine protein expression and localization of type I procollagen, the precursor of type I collagen fibrils. Real-time polymerase chain reaction was used to determine gene expression levels. RESULTS: In control (hip) and stretched normal-appearing perilesional abdominal skin, dermal collagen fibrils were organized as tightly packed, interwoven bundles. In SG, collagen bundles appeared markedly separated, especially in the mid-to-deep dermis. In the spaces separating these bundles, loosely packed wavy collagen fibrils lacking organization as bundles were present. These disorganized fibrils persisted into the postpartum period and failed to form densely packed bundles. Numerous large fibroblasts displaying type I procollagen expression were in close proximity to the disorganized fibrils, suggesting that the fibrils are newly synthesized. Supporting this possibility, immunostaining and gene expression of type I procollagen were increased throughout the dermis of SG. CONCLUSIONS: Early SG display marked separation of collagen bundles and emergence of disorganized collagen fibrils that fail to form bundles. These alterations may reflect ineffective repair of collagen bundles disrupted by intense skin stretching. Persistent disruption of the collagenous extracellular matrix likely promotes formation and atrophy of SG.


Assuntos
Doenças do Colágeno/patologia , Complicações na Gravidez/patologia , Estrias de Distensão/patologia , Estudos de Casos e Controles , Doenças do Colágeno/metabolismo , Colágeno Tipo I/metabolismo , Feminino , Colágenos Fibrilares/fisiologia , Fibroblastos/metabolismo , Humanos , Gravidez , Complicações na Gravidez/metabolismo , Pró-Colágeno/biossíntese , Pele/irrigação sanguínea , Estrias de Distensão/metabolismo , Adulto Jovem
4.
J Cosmet Dermatol ; 16(2): 279-286, 2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28374517

RESUMO

BACKGROUND: Striae distensae (SD), a type of dermal scarring, are psychologically disappointing. To date, information and scientific research behind the role of hormonal factors in the development of SD are still unclear. It is vital to understand striae to offer patients the best therapeutic options. OBJECTIVES: To investigate early alterations regarding the expression of estrogen, androgen, and glucocorticoid receptors (estrogen receptor (ER), androgen receptor (AR), glucocorticoid receptor (GR) in skin samples of multigravida (MG) and nulligravida (NG) cases and to compare them with normal controls. METHODS: This study included 30 subjects (10 MG and 10 NG cases with early SD and 10 healthy controls). Biopsies from SD lesions, perilesional normal skin of cases and normal skin of controls were examined immunohistochemically for ER, AR, and GR expression using immune peroxidase technique. RESULTS: Comparing MG and NG with controls, ER expression appeared reduced in MG and NG (P-value<.001), AR was elevated in MG (P-value<.05) with no considerable difference in NG (P-value>.05), while GR was elevated in both MG and NG (P-value<.05). On comparing perilesional skin with SD lesions in each of MG and NG groups, SD lesions revealed reduced ER expression in both groups (P-value<.05), whereas in MG group: AR expression was elevated with no difference detected regarding GR (P-value˃.05); meanwhile in NG, elevated expression in both AR and GR expression was noted (P-Value<.001) CONCLUSIONS: Striae distansae lesions demonstrated a significant increase in the expression of AR and GR and a declined expression of ER indicating their involvement in the development of early SD.


Assuntos
Receptores Androgênicos/análise , Receptores Estrogênicos/análise , Receptores de Glucocorticoides/análise , Estrias de Distensão/patologia , Adulto , Feminino , Número de Gestações , Humanos , Imuno-Histoquímica , Gravidez , Receptores Androgênicos/biossíntese , Receptores Estrogênicos/biossíntese , Receptores de Glucocorticoides/biossíntese , Estrias de Distensão/metabolismo , Adulto Jovem
5.
Br J Dermatol ; 173(6): 1420-30, 2015 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-26179468

RESUMO

BACKGROUND: Striae gravidarum (SG), or 'stretch marks' of pregnancy, begin as erythematous streaks, and mature over months to years to become permanent scar-like bands that may be hypopigmented, atrophic and lax. OBJECTIVES: To investigate early molecular alterations that may promote laxity of mature SG, we investigated the dermal elastic fibre network, which provides human skin with elastic properties. METHODS: We obtained skin samples of newly developed, erythematous abdominal SG in healthy pregnant women. The elastic fibre network was examined by Verhoeff elastic staining and immunofluorescence staining of skin sections. Gene expression was measured by real-time polymerase chain reaction. RESULTS: The normal elastic fibre network appeared markedly disrupted in SG, compared with perilesional abdominal skin or control (normal-appearing hip skin). This disruption was accompanied by the emergence of short, disorganized, thin, thread-like 'fibrils', which were observed prominently in the mid-to-deep dermis. These fibrils were rich in tropoelastin (the main component of normal elastic fibres), and persisted into the postpartum period without forming normal-appearing elastic fibres. The emergence of these fibrils was accompanied by increased gene expression of tropoelastin and fibrillin-1, but not other elastic fibre components, including fibrillin-2 and fibulin-1, -2 or -5. CONCLUSIONS: In early SG, the elastic fibre network appears markedly disrupted, and newly synthesized tropoelastin-rich fibrils emerge, likely as a result of uncoordinated synthesis of elastic fibre components. Because they are thin and disorganized, tropoelastin-rich fibrils likely do not function as normal elastic fibres do. These observations provide the foundations for elucidating pathogenic mechanisms by which laxity may develop in SG.


Assuntos
Tecido Elástico/patologia , Estrias de Distensão/patologia , Doenças do Colágeno/patologia , Tecido Elástico/metabolismo , Feminino , Humanos , Gravidez , Transtornos Puerperais/metabolismo , Transtornos Puerperais/patologia , Estrias de Distensão/metabolismo , Tropoelastina/metabolismo , Adulto Jovem
6.
J Cosmet Dermatol ; 14(4): 336-46, 2015 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-26147455

RESUMO

Striae distensae is a challenging cosmetic problem for which various treatment modalities have been applied. To compare between the efficacy and tolerability of intradermal injection of autologous platelet-rich plasma (PRP) vs. microdermabrasion in the treatment of striae distensae. Sixty-eight patients with striae distensae were randomly assigned to three groups according to therapeutic modalities. Patients of group I were treated by intradermal injection of PRP alone, patients of group II were treated with microdermabrasion alone, and patients of group III were treated with combination of intradermal PRP and microdermabrasion in the same session. Each patient underwent maximum of six sessions at 2-week interval. Skin biopsies were taken from some patients at baseline, and 3 months after the last sessions stained with hematoxylin and eosin stain, Masson trichrome, orceun, and Van Gieson stains to study of histopathological changes and efficacy of treatment. There was significant clinical improvement of striae distensae in patients treated with PRP injection and patients treated with combination of PRP and microdermabrasion when compared with patients treated with microdermabrasion. However, combination of PRP and microdermabrasion in the same session showed better results in short duration. Collagen and elastic fibers were markedly increased in the dermis at the end of treatment sessions. Platelet-rich plasma alone is more effective than microdermabrasion alone in the treatment of striae distensae, but it is better to use the combination of both for more and rapid efficacy. However, each one of them is well tolerated by the patients, safe and cost effective.


Assuntos
Dermabrasão/métodos , Plasma Rico em Plaquetas , Estrias de Distensão/patologia , Estrias de Distensão/terapia , Adolescente , Adulto , Biópsia por Agulha , Colágeno/metabolismo , Terapia Combinada , Dermabrasão/efeitos adversos , Feminino , Humanos , Injeções Intradérmicas , Masculino , Satisfação do Paciente , Estrias de Distensão/metabolismo , Resultado do Tratamento , Adulto Jovem
7.
Dermatol Surg ; 39(10): 1452-8, 2013 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-23895146

RESUMO

BACKGROUND: Striae distensae are dermal scars with flattening and atrophy of the epidermis. OBJECTIVE: To evaluate the efficacy and safety of combination therapy with fractionated microneedle radiofrequency (RF) and fractional carbon dioxide (CO2) laser in the treatment of striae distensae. MATERIALS AND METHODS: Thirty patients (30 female; mean age 33, range 21-51, Fitzpatrick skin type IV) with moderate to severe striae distensae were enrolled in this study. Patients were divided into three groups: fractional CO2 laser only (n = 10), microneedle RF only (n = 10), and combination (n = 10). RESULTS: Improvement was evaluated using a visual analogue scale (range 1-4). Mean clinical improvement score of the dermatologist was 2.2 in the fractional CO2 laser-treated group, 1.8 in the microneedle RF-treated group, and 3.4 in the combination group. Through skin biopsy, we observed thickened epidermis and a clear increase in the number of collagen fibers in the microneedle RF- and fractional CO2 combination-treated sites. Consistent with these results, greater expression of transforming growth factor-ß1 and stratifin was observed in treated sites. CONCLUSION: Combination therapy of fractionated microneedle RF and fractional CO2 laser is a safe treatment protocol with a positive therapeutic effect on striae distensae.


Assuntos
Ablação por Cateter , Lasers de Gás/uso terapêutico , Estrias de Distensão/cirurgia , Proteínas 14-3-3/metabolismo , Adulto , Biomarcadores Tumorais/metabolismo , Dióxido de Carbono , Terapia Combinada , Exonucleases/metabolismo , Exorribonucleases , Feminino , Humanos , Pessoa de Meia-Idade , República da Coreia , Estrias de Distensão/metabolismo , Estrias de Distensão/patologia , Fator de Crescimento Transformador beta1/metabolismo , Resultado do Tratamento , Adulto Jovem
8.
J Cosmet Laser Ther ; 15(6): 305-9, 2013 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-23464621

RESUMO

Abstract Intense pulsed light (IPL) has been used extensively to treat striae distensae (SD). Twenty-four patients (21 females and 3 males) with age ranging between 15-42 years with a mean of 24.2 ± 5.89 years were included in this study. All patients were treated with IPL for five sessions and were given 4-week interval between the sessions. Formalin-fixed, paraffin- embedded skin biopsies were cut into 5-µm-thick sections from patients before and after treatments. They were stained with hematoxylin and eosin (H&E) and collagen with Masson trichrome stain. Histological examination of SD revealed epidermal thinning, flattened rete ridges, and fragmentation with degeneration of the collagen and elastic fibers. After IPL treatments, collagen expression was found also to be increased in a highly significant manner and P values were < 0.001 after IPL treatments. The use of synchrotron infrared (IR) microspectroscopy also revealed that amide 1 and beta sheets were expressed in a significant manner after IPL treatment. In conclusion, it was demonstrated that IPL can treat striae through fibroblast stimulation which led to protein expression especially collagen stimulation, and it seems that it is a promising therapeutic modality for striae with minimal side effects.


Assuntos
Fibroblastos , Terapia de Luz Pulsada Intensa , Pele/citologia , Espectroscopia de Infravermelho com Transformada de Fourier , Estrias de Distensão/patologia , Estrias de Distensão/terapia , Adolescente , Adulto , Colágeno/biossíntese , Colágeno/ultraestrutura , Feminino , Humanos , Masculino , Microscopia , Estrutura Secundária de Proteína , Estrias de Distensão/metabolismo , Adulto Jovem
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