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1.
ACS Appl Mater Interfaces ; 13(33): 40070-40078, 2021 Aug 25.
Artigo em Inglês | MEDLINE | ID: mdl-34387999

RESUMO

Aminothiols are closely related to chronic kidney disease, but little is known regarding levels of related aminothiols in the urine of immunoglobulin A vasculitis with nephritis (IgAVN) patients. Herein, a well-defined core-shell Zr-based metal-organic framework (Zr-MOF) composite SiO2@50Benz-Cys was constructed as a mercury ion affinity material via a solvent-assisted ligand exchange strategy for the selective extraction and enrichment of low-concentration aminothiols in IgAVN patient urine. SiO2@50Benz-Cys was competent to enrich the total glutathione (GSH) and total homocysteine (Hcy) in virtue of the excellent affinity after chelation with mercury ions. The extraction efficiencies were closely related to the pH, dithiothreitol amount, and the dose of functional Zr-MOF. Coupled with HPLC-MS/MS in optimized conditions, GSH and Hcy were determined with low detection limits of 0.5 and 1 nmol L-1, respectively. The recoveries of GSH and Hcy for the urine sample at three spiked levels were in the range of 85.3-105% and 79.5-103%, which showed good precision and accuracy. Benefiting from the matrix interference elimination in the process of extraction, the simultaneous detection of aminothiols in the urine of the healthy group and immunoglobulin A vasculitis (IgAV) and IgAVN patients was successfully carried out, suggesting that the Zr-MOF and the robust method together provided a potential application in the analysis of urinary biomolecules. The analysis of variance (ANOVA) showed that the levels of GSH and Hcy had significant differences between the patients and the control. This work is very valuable as it provides a better understanding of concentration alterations of GSH and Hcy in urine involved with IgAVN for clinical research.


Assuntos
Glutationa/urina , Homocisteína/urina , Estruturas Metalorgânicas/química , Nefrite/diagnóstico , Zircônio/química , Técnicas Biossensoriais , Cromatografia Líquida de Alta Pressão , Humanos , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Dióxido de Silício , Coloração e Rotulagem/métodos , Compostos de Sulfidrila/química , Espectrometria de Massas em Tandem
2.
J Chem Phys ; 154(24): 244703, 2021 Jun 28.
Artigo em Inglês | MEDLINE | ID: mdl-34241364

RESUMO

Cancer remains hard to treat, partially due to the non-specificity of chemotherapeutics. Metal-organic frameworks (MOFs) are promising carriers for targeted chemotherapy, yet, to date, there have been few detailed studies to systematically enhance drug loading while maintaining controlled release. In this work, we investigate which molecular simulation methods best capture the experimental uptake and release of cisplatin from UiO-66 and UiO-66(NH2). We then screen a series of biocompatible, pH-sensitive zeolitic imidazolate frameworks (ZIFs) for their ability to retain cisplatin in healthy parts of the patient and release it in the vicinity of a tumor. Pure-component GCMC simulations show that the maximum cisplatin loading depends on the pore volume. To achieve this maximum loading in the presence of water, either the pore size needs to be large enough to occupy both cisplatin and its solvation shell or the MOF-cisplatin interaction must be more favorable than the cisplatin-shell interaction. Both solvated and non-solvated simulations show that cisplatin release rates can be controlled by either decreasing the pore limiting diameters or by manipulating framework-cisplatin interaction energies to create strong, dispersed adsorption sites. The latter method is preferable if cisplatin loading is performed from solution into a pre-synthesized framework as weak interaction energies and small pore window diameters will hinder cisplatin uptake. Here, ZIF-82 is most promising. If it is possible to load cisplatin during crystallization, ZIF-11 would outcompete the other MOFs screened as cisplatin cannot pass through its pore windows; therefore, release rates would be purely driven by the pH triggered framework degradation.


Assuntos
Cisplatino/química , Imidazóis/química , Estruturas Metalorgânicas/química , Zeolitas/química , Modelos Moleculares
3.
Chem Commun (Camb) ; 57(60): 7362-7365, 2021 Jul 27.
Artigo em Inglês | MEDLINE | ID: mdl-34196343

RESUMO

A facile strategy was introduced for room-temperature controllable synthesis of hierarchically flower-like hollow COFs (FHF-COFs). Furthermore, the universality for synthesis of the HFH-COFs was validated by altering the building units. Inspired by the unique morphology, extremely large surface area and good chemical stability, HFH-COFs could serve as an attractive adsorption probe by loading with gold nanoparticles and be applied to enrichment of brain natriuretic peptide from human serum. This work opens up a whole new approach for controllable synthesis of the HFH-COFs at room temperature and expands the application of COFs as a promising enrichment probe for complex biological samples.


Assuntos
Estruturas Metalorgânicas/química , Peptídeo Natriurético Encefálico/isolamento & purificação , Adsorção , Ouro/química , Humanos , Limite de Detecção , Nanopartículas Metálicas/química , Estruturas Metalorgânicas/síntese química , Peptídeo Natriurético Encefálico/sangue , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
4.
Nat Commun ; 12(1): 4556, 2021 07 27.
Artigo em Inglês | MEDLINE | ID: mdl-34315880

RESUMO

The potential applications of covalent organic frameworks (COFs) can be further developed by encapsulating functional nanoparticles within the frameworks. However, the synthesis of monodispersed core@shell structured COF nanocomposites without agglomeration remains a significant challenge. Herein, we present a versatile dual-ligand assistant strategy for interfacial growth of COFs on the functional nanoparticles with abundant physicochemical properties. Regardless of the composition, geometry or surface properties of the core, the obtained core@shell structured nanocomposites with controllable shell-thickness are very uniform without agglomeration. The derived bowl-shape, yolk@shell, core@satellites@shell nanostructures can also be fabricated delicately. As a promising type of photosensitizer for photodynamic therapy (PDT), the porphyrin-based COFs were grown onto upconversion nanoparticles (UCNPs). With the assistance of the near-infrared (NIR) to visible optical property of UCNPs core and the intrinsic porosity of COF shell, the core@shell nanocomposites can be applied as a nanoplatform for NIR-activated PDT with deep tissue penetration and chemotherapeutic drug delivery.


Assuntos
Estruturas Metalorgânicas/química , Nanopartículas/química , Animais , Antineoplásicos/farmacologia , Linhagem Celular Tumoral , Sistemas de Liberação de Medicamentos , Feminino , Células Endoteliais da Veia Umbilical Humana/efeitos dos fármacos , Células Endoteliais da Veia Umbilical Humana/metabolismo , Humanos , Ligantes , Camundongos Endogâmicos BALB C , Nanocompostos/química , Nanopartículas/ultraestrutura , Fotoquimioterapia , Porfirinas/química , Dióxido de Silício/química , Tela Subcutânea/efeitos dos fármacos , Tela Subcutânea/patologia
5.
Nat Commun ; 12(1): 4039, 2021 06 30.
Artigo em Inglês | MEDLINE | ID: mdl-34193867

RESUMO

The controlled assembly of nanomaterials into desired architectures presents many opportunities; however, current preparations lack spatial precision and versatility in developing complex nano-architectures. Inspired by the amphiphilic nature of surfactants, we develop a facile approach to guide nanomaterial integration - spatial organization and distribution - in metal-organic frameworks (MOFs). Named surfactant tunable spatial architecture (STAR), the technology leverages the varied interactions of surfactants with nanoparticles and MOF constituents, respectively, to direct nanoparticle arrangement while molding the growing framework. By surfactant matching, the approach achieves not only tunable and precise integration of diverse nanomaterials in different MOF structures, but also fast and aqueous synthesis, in solution and on solid substrates. Employing the approach, we develop a dual-probe STAR that comprises peripheral working probes and central reference probes to achieve differential responsiveness to biomarkers. When applied for the direct profiling of clinical ascites, STAR reveals glycosylation signatures of extracellular vesicles and differentiates cancer patient prognosis.


Assuntos
Biomarcadores Tumorais/metabolismo , Técnicas Biossensoriais/métodos , Neoplasias Colorretais/diagnóstico , Vesículas Extracelulares/metabolismo , Estruturas Metalorgânicas/química , Nanoestruturas/química , Tensoativos/química , Ascite/metabolismo , Neoplasias Colorretais/metabolismo , Glicosilação , Humanos , Prognóstico
6.
Molecules ; 26(12)2021 Jun 09.
Artigo em Inglês | MEDLINE | ID: mdl-34207684

RESUMO

Biodiesel is a promising candidate for sustainable and renewable energy and extensive research is being conducted worldwide to optimize its production process. The employed catalyst is an important parameter in biodiesel production. Metal-organic frameworks (MOFs), which are a set of highly porous materials comprising coordinated bonds between metals and organic ligands, have recently been proposed as catalysts. MOFs exhibit high tunability, possess high crystallinity and surface area, and their order can vary from the atomic to the microscale level. However, their catalytic sites are confined inside their porous structure, limiting their accessibility for biodiesel production. Modification of MOF structure by immobilizing enzymes or ionic liquids (ILs) could be a solution to this challenge and can lead to better performance and provide catalytic systems with higher activities. This review compiles the recent advances in catalytic transesterification for biodiesel production using enzymes or ILs. The available literature clearly indicates that MOFs are the most suitable immobilization supports, leading to higher biodiesel production without affecting the catalytic activity while increasing the catalyst stability and reusability in several cycles.


Assuntos
Biocombustíveis/análise , Biotecnologia/métodos , Enzimas Imobilizadas/química , Líquidos Iônicos/química , Estruturas Metalorgânicas/química , Catálise , Esterificação , Porosidade
7.
Int J Mol Sci ; 22(11)2021 Jun 03.
Artigo em Inglês | MEDLINE | ID: mdl-34205199

RESUMO

Lead detection for biological environments, aqueous resources, and medicinal compounds, rely mainly on either utilizing bulky lab equipment such as ICP-OES or ready-made sensors, which are based on colorimetry with some limitations including selectivity and low interference. Remote, rapid and efficient detection of heavy metals in aqueous solutions at ppm and sub-ppm levels have faced significant challenges that requires novel compounds with such ability. Here, a UiO-66(Zr) metal-organic framework (MOF) functionalized with SO3H group (SO3H-UiO-66(Zr)) is deposited on the end-face of an optical fiber to detect lead cations (Pb2+) in water at 25.2, 43.5 and 64.0 ppm levels. The SO3H-UiO-66(Zr) system provides a Fabry-Perot sensor by which the lead ions are detected rapidly (milliseconds) at 25.2 ppm aqueous solution reflecting in the wavelength shifts in interference spectrum. The proposed removal mechanism is based on the adsorption of [Pb(OH2)6]2+ in water on SO3H-UiO-66(Zr) due to a strong affinity between functionalized MOF and lead. This is the first work that advances a multi-purpose optical fiber-coated functional MOF as an on-site remote chemical sensor for rapid detection of lead cations at extremely low concentrations in an aqueous system.


Assuntos
Chumbo/isolamento & purificação , Metais Pesados/isolamento & purificação , Compostos Organometálicos/química , Ácidos Ftálicos/química , Poluentes Químicos da Água/isolamento & purificação , Humanos , Chumbo/química , Estruturas Metalorgânicas/química , Metais Pesados/química , Fibras Ópticas , Água/química , Poluentes Químicos da Água/química , Zircônio/química
8.
Int J Mol Sci ; 22(11)2021 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-34206067

RESUMO

Nanozymes, nanomaterials with enzyme-like activities, are becoming powerful competitors and potential substitutes for natural enzymes because of their excellent performance. Nanozymes offer better structural stability over their respective natural enzymes. In consequence, nanozymes exhibit promising applications in different fields such as the biomedical sector (in vivo diagnostics/and therapeutics) and the environmental sector (detection and remediation of inorganic and organic pollutants). Prussian blue nanoparticles and their analogues are metal-organic frameworks (MOF) composed of alternating ferric and ferrous irons coordinated with cyanides. Such nanoparticles benefit from excellent biocompatibility and biosafety. Besides other important properties, such as a highly porous structure, Prussian blue nanoparticles show catalytic activities due to the iron atom that acts as metal sites for the catalysis. The different states of oxidation are responsible for the multicatalytic activities of such nanoparticles, namely peroxidase-like, catalase-like, and superoxide dismutase-like activities. Depending on the catalytic performance, these nanoparticles can generate or scavenge reactive oxygen species (ROS).


Assuntos
Ferrocianetos/química , Estruturas Metalorgânicas/química , Nanopartículas/química , Nanoestruturas/química , Catalase , Catálise , Complexos de Coordenação/química , Ferro/química , Oxirredução , Peroxidase , Espécies Reativas de Oxigênio
9.
Molecules ; 26(12)2021 Jun 16.
Artigo em Inglês | MEDLINE | ID: mdl-34208577

RESUMO

Metal-organic frameworks (MOFs) have been rapidly developed for their broad applications in many different chemistry and materials fields. In this work, a multi-dentate building block 5-(4-(tetrazol-5-yl)phenyl)-isophthalic acid (H3L) containing tetrazole and carbolxylate moieties was employed for the synthesis of a two-dimensional (2D) lanthanide MOF [La(HL)(DMF)2(NO3)] (DMF = N,N-dimethylformamide) (1) under solvothermal condition. The fluorescent sensing application of 1 was investigated. 1 exhibits high sensitivity recognition for antibiotic nitrofurantoin (Ksv: 3.0 × 103 M-1 and detection limit: 17.0 µM) and amino acid l-tyrosine (Ksv: 1.4 × 104 M-1 and detection limit: 3.6 µM). This work provides a feasible detection platform of 2D MOFs for highly sensitive discrimination of antibiotics and amino acids.


Assuntos
Elementos da Série dos Lantanídeos/química , Nitrofurantoína/química , Tirosina/química , Antibacterianos/química , Cristalografia por Raios X/métodos , Corantes Fluorescentes/química , Estruturas Metalorgânicas/química , Nitrofurantoína/metabolismo , Tirosina/metabolismo
10.
ACS Appl Mater Interfaces ; 13(27): 32058-32066, 2021 Jul 14.
Artigo em Inglês | MEDLINE | ID: mdl-34197071

RESUMO

Exploration of novel material platforms to protect biological preservatives and realize intelligent regulation during fermentation is of great significance in industry. Herein, we established an intelligent responsive platform by introducing antimicrobial biomolecules (nisin) into rationally designed covalent organic frameworks (COFs), resulting in a new type of "smart formulation", which could responsively inhibit microbial contamination and ensure the orderly progression of the fermentation process. The encapsulated biomolecules retained their activity while exhibiting enhanced stability and pH-responsive releasing process (100% bacteriostatic efficiency at a pH of 3), which can ingeniously adapt to the environmental variation during the fermentation process and smartly fulfill the regulation needs. Moreover, the nisin@COF composites would not affect the fermentation strains. This study will pave a new avenue for the preparation of highly efficient and intelligent antimicrobial agents for the regulation of the fermentation process and play valuable roles in the drive toward green and sustainable biomanufacturing.


Assuntos
Fermentação/efeitos dos fármacos , Estruturas Metalorgânicas/química , Nisina/química , Nisina/farmacologia , Desenho de Fármacos , Concentração de Íons de Hidrogênio
11.
ACS Appl Mater Interfaces ; 13(27): 31710-31724, 2021 Jul 14.
Artigo em Inglês | MEDLINE | ID: mdl-34213303

RESUMO

In this study, we have designed a three-fluorophore-labeled Y-shaped DNAzyme with a high catalytic cleavage activity and a three-dimensional (3D) MOF-MoS2NB (metal-organic framework fused with molybdenum disulfide nanobox), which was synthesized as an efficient quencher of the fluorescent biosensor. The synthesized porous 3D MOF-MoS2NBs and Y-shaped DNAzyme exhibited a good analytical response toward the simultaneous multiple detections of Hg2+, Ni2+, and Ag+ ions over the other coexisting metal ions. More specifically, the three kinds of enzyme aptamer and substrate aptamer (SA) were hybridized and annealed to form the Y-shaped DNAzyme structure and labeled with three different fluorophores such as FAM, TAMRA, and ROX over the 3'-end of SA. When the targets were induced, the DNAzyme was triggered to cleave the fluorophore-labeled SAs. Then, the cleaved SAs (FAM-SA, TAMRA-SA, and ROX-SA) were adsorbed on the 3D MOF-MoS2NB surface to quench the fluorescence signal due to a noncovalent interaction (van der Waals and π-π stacking interaction), which transmuted the fluorescence on-state to off-state. As a result, the fluorescence assay confiscated the high selectivity and sensitivity for the target analytes of Hg2+, Ni2+, and Ag+ ions achieved for the detection limits of 0.11 nM, 7.8 µM, and 0.25 nM, respectively. Accordingly, the sensitivity of the developed sensor was explored with a better lower detection limit than the previously reported biosensors. The utility of the designed Y-shaped DNAzyme may find a broad field of application in real water sample analysis with interfering contaminants.


Assuntos
Biocatálise , Técnicas Biossensoriais/métodos , DNA Catalítico/metabolismo , Dissulfetos/química , Corantes Fluorescentes/química , Estruturas Metalorgânicas/química , Metais Pesados/análise , Molibdênio/química , Adsorção , Catálise , DNA Catalítico/química , Limite de Detecção , Mercúrio/análise , Níquel/análise , Prata/análise
12.
Molecules ; 26(13)2021 Jun 23.
Artigo em Inglês | MEDLINE | ID: mdl-34201878

RESUMO

Composites of metal-organic frameworks and carbon materials have been suggested to be effective materials for the decomposition of chemical warfare agents. In this study, we synthesized UiO-66-NH2/zeolite-templated carbon (ZTC) composites for the adsorption and decomposition of the nerve agents sarin and soman. UiO-66-NH2/ZTC composites with good dispersion were prepared via a solvothermal method. Characterization studies showed that the composites had higher specific surface areas than pristine UiO-66-NH2, with broad pore size distributions centered at 1-2 nm. Owing to their porous nature, the UiO-66-NH2/ZTC composites could adsorb more water at 80% relative humidity. Among the UiO-66-NH2/ZTC composites, U0.8Z0.2 showed the best degradation performance. Characterization and gas adsorption studies revealed that beta-ZTC in U0.8Z0.2 provided additional adsorption and degradation sites for nerve agents. Among the investigated materials, including the pristine materials, U0.8Z0.2 also exhibited the best protection performance against the nerve agents. These results demonstrate that U0.8Z0.2 has the optimal composition for exploiting the degradation performance of pristine UiO-66-NH2 and the adsorption performance of pristine beta-ZTC.


Assuntos
Carbono/química , Estruturas Metalorgânicas/química , Agentes Neurotóxicos/química , Compostos Organometálicos/química , Ácidos Ftálicos/química , Zeolitas/química , Adsorção , Estruturas Metalorgânicas/síntese química , Estruturas Metalorgânicas/ultraestrutura , Microscopia Eletrônica de Varredura , Porosidade , Sarina/química , Soman/química , Espectroscopia de Infravermelho com Transformada de Fourier , Água/química , Difração de Raios X
13.
Se Pu ; 39(3): 205-210, 2021 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-34227302

RESUMO

Protein glycosylation is a ubiquitous and important biological process involved in various molecular functions and biological pathways. It also yields important biomarkers for clinical diagnoses. However, glycopeptide analysis is challenging due to low abundance, low ionization efficiency, and glycan heterogeneity. In the present study, a method based on hydrophilic interaction liquid chromatography (HILIC) was developed for the selective enrichment of glycopeptides using a novel metal-organic framework (MOF) nanocomposite (AuGC/ZIF-8). Dual functionalization with glutathione and cysteine has resulted in an ultra-hydrophilic MOF, with synergistic effects and lower steric hindrance, providing more affinity sites for the glycopeptide enrichment. Horseradish peroxidase (HRP) was used as a model glycoprotein, and AuGC/ZIF-8 was used to enrich glycopeptides prior to analysis by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). AuGC/ZIF-8 displayed outstanding performance at enriching HRP glycopeptides, with high enrichment capacity (250 µg/mg), high selectivity in mixtures containing bovine serum albumin (BSA) (HRP-BSA (1∶200, mass ratio)), and high sensitivity at very low content (0.3 ng/µL). Thus this MOF holds promise for in-depth, comprehensive glycoproteomic and related analysis.


Assuntos
Glicopeptídeos/química , Estruturas Metalorgânicas , Nanocompostos , Cromatografia Líquida , Peroxidase do Rábano Silvestre , Interações Hidrofóbicas e Hidrofílicas , Estruturas Metalorgânicas/química , Soroalbumina Bovina , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
14.
Inorg Chem ; 60(14): 10729-10737, 2021 Jul 19.
Artigo em Inglês | MEDLINE | ID: mdl-34197115

RESUMO

The pharmacological profile of medicinally relevant Ru(III) coordination compounds has been ascribed to their interactions with proteins, as several studies have provided evidence that DNA is not the primary target. In this regard, numerous spectroscopic and crystallographic studies have indicated that the Ru(III) ligands play an important role in determining the metal binding site, acting as the recognition element in the early stages of the protein-complex formation. Herein, we present a series of near-atomic-resolution X-ray crystal structures of the adducts formed between the antimetastatic metallodrug imidazolium trans-[tetrachlorido(S-dimethyl sufoxide)(1H-imidazole)ruthenate(III)] (NAMI-A) and hen egg-white lysozyme (HEWL). These structures elucidate a series of binding events starting from the noncovalent interaction of intact NAMI-A ions with HEWL (1.5 h), followed by the stepwise exchange of all Ru ligands except for 1H-imidazole (26 h) to the final "ruthenated" protein comprising one aquated Ru ion coordinated to histidine-15 of HEWL (98 h). Our structural data clearly support a two-step mechanism of protein ruthenation, illustrating the ligand-mediated recognition step of the process.


Assuntos
Antineoplásicos/química , Antineoplásicos/farmacologia , Estruturas Metalorgânicas/química , Estruturas Metalorgânicas/farmacologia , Muramidase/química , Rutênio/química , Linhagem Celular Tumoral , Cristalografia por Raios X , Humanos , Imidazóis/química , Modelos Moleculares , Metástase Neoplásica , Conformação Proteica
15.
J Photochem Photobiol B ; 222: 112274, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34330082

RESUMO

Photodynamic therapy (PDT) has gained much attention in tumor therapy because of its special advantages. PDT heavily depends on the oxygen, yet the tumor microenvironment (TME) is a hypoxic and acid milieu, which weakens the PDT effect. Based on the consideration that the TME deteriorated by the PDT oxygen consumption could activate the hypoxic-sensitive small-molecule drug, we designed and prepared an integrated nanocomposite including zirconium ion metal organic framework (carrier), pyropheophorbide-a (PPa, photosensitizer), and 6-amino flavone (AF, hypoxic-sensitive drug), aiming to exert a cascaded PDT-chemotherapy (CT) antitumor effect and to solve the hypoxic challenge. The prepared nanocomposite showed great stability under the physiological (pH 7.4) condition and could continuously release PPa and AF under slightly acidic pH condition (pH 6.4), suggesting a tumor microenvironment responsive feature. Systematical in vitro and in vivo researches under various conditions (light, dark, hypoxic and normoxic) have showed that the obtained Zr-MOF@PPa/AF@PEG nanoparticles (NPs) had good biocompatibility and could achieve efficient antitumor effects based on PDT- chemotherapy (CT) cascade process. Finally, bright red fluorescence was observed in the tumor cells after internalization implying an application potential in tumor imaging.


Assuntos
Clorofila/análogos & derivados , Flavonoides/química , Estruturas Metalorgânicas/química , Nanocompostos/química , Fotoquimioterapia/métodos , Nanomedicina Teranóstica , Animais , Materiais Biocompatíveis/química , Materiais Biocompatíveis/farmacologia , Materiais Biocompatíveis/uso terapêutico , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Clorofila/química , Clorofila/metabolismo , Clorofila/uso terapêutico , Liberação Controlada de Fármacos , Feminino , Flavonoides/metabolismo , Flavonoides/uso terapêutico , Humanos , Camundongos , Camundongos Endogâmicos BALB C , Nanocompostos/uso terapêutico , Nanocompostos/toxicidade , Neoplasias/tratamento farmacológico , Neoplasias/patologia , Polietilenoglicóis/química , Oxigênio Singlete/metabolismo , Microambiente Tumoral , Zircônio/química
16.
J Chromatogr A ; 1651: 462301, 2021 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-34107399

RESUMO

In this work, a core-shell structured magnetic covalent organic framework named as M-TpDAB was constructed with 3,3'-diaminobenzidine (DAB) and 1,3,5-triformylphloroglucinol (Tp) as building units. M-TpDAB was characterized by infrared spectroscopy, nitrogen adsorption-desorption isotherms, powder X-ray diffraction, scanning electron microscopy and transmission electron microscopy. Using the M-TpDAB as adsorbent, a simple and highly effective method was proposed for preconcentrating phenylurea herbicides before high performance liquid-phase chromatography analysis. In the optimized conditions, a good linearity was achieved within the range of 0.15-100 ng mL-1 for water sample, 1.0-100.0 ng mL-1 for tea drink samples. The limits of detection for the analytes were 0.05-0.15 ng mL-1 for water sample and 0.30-0.50 ng mL-1 for drink samples. Satisfactory recoveries of spiked target compounds were in the range of 84.6%-105% for water sample and 80.3%-102% for tea drink samples. Finally, the M-TpDAB based method was successfully used to determine phenylurea herbicides in tea drinks and water samples, demonstrating a good alternative for analyzing trace level of phenylurea herbicides in water samples.


Assuntos
Herbicidas/isolamento & purificação , Estruturas Metalorgânicas/química , Compostos de Fenilureia/isolamento & purificação , Extração em Fase Sólida/métodos , Água/química , Adsorção , Cromatografia Líquida de Alta Pressão , Água Potável/química , Herbicidas/análise , Fenômenos Magnéticos , Magnetismo/métodos , Compostos de Fenilureia/análise , Difração de Raios X
17.
Chem Commun (Camb) ; 57(55): 6776-6779, 2021 Jul 08.
Artigo em Inglês | MEDLINE | ID: mdl-34137399

RESUMO

We report the straightforward synthesis of ATP-responsive nanoscale metal azolate framework-7 (MAF-7) for gene/drug codelivery. The MAF-7 functions as (i) the armour to preserve DNAzymes, (ii) an ATP scavenger to lower the intracellular ATP level, and (iii) a built-in Zn2+ arsenal to initiate the biocatalysis of DNAzymes, ultimately inhibiting P-gp expression to enhance chemotherapy.


Assuntos
Membro 1 da Subfamília B de Cassetes de Ligação de ATP/metabolismo , Antineoplásicos/química , Azóis/química , Portadores de Fármacos/química , Resistência a Múltiplos Medicamentos/efeitos dos fármacos , Estruturas Metalorgânicas/química , Nanoestruturas/química , Trifosfato de Adenosina/metabolismo , Antineoplásicos/farmacologia , Linhagem Celular Tumoral , Humanos , Espaço Intracelular/efeitos dos fármacos , Espaço Intracelular/metabolismo
18.
Chem Commun (Camb) ; 57(58): 7152-7155, 2021 Jul 20.
Artigo em Inglês | MEDLINE | ID: mdl-34184013

RESUMO

Glass micropipettes are easy to fabricate, have excellent flexibility and stable properties. HKUST-1 and MIL-68(In) are in situ grown in the tip of a micropipette to construct porous nanochannels. After absorbing H2S, the MIL-68(In)-based nanochannel shows effective metal ion responsiveness for Hg2+-detection.


Assuntos
Sulfeto de Hidrogênio/análise , Estruturas Metalorgânicas/química , Adsorção , Vidro/química , Sulfeto de Hidrogênio/isolamento & purificação , Nanoestruturas/química , Porosidade
19.
Chem Commun (Camb) ; 57(59): 7240-7243, 2021 Jul 28.
Artigo em Inglês | MEDLINE | ID: mdl-34190264

RESUMO

Herin, we report a Cu(ii)-porphyrin-derived nanoscale COF, which can be triggered by endogenous H2S via an intracellular sulfidation reaction to generate a metal-free COF-photosensitizer for PDT against H2S-enriched colon tumors with controllable singlet oxygen release; meanwhile in situ generated CuS can be synchronously used as a photothermal agent for PTT.


Assuntos
Neoplasias do Colo/terapia , Sulfeto de Hidrogênio/química , Estruturas Metalorgânicas/química , Fototerapia/métodos , Animais , Neoplasias do Colo/tratamento farmacológico , Neoplasias do Colo/patologia , Cobre/química , Células HCT116 , Humanos , Raios Infravermelhos , Camundongos , Microscopia Confocal , Fármacos Fotossensibilizantes/química , Fármacos Fotossensibilizantes/uso terapêutico , Porfirinas/química , Oxigênio Singlete/metabolismo , Transplante Heterólogo
20.
Inorg Chem ; 60(16): 11739-11744, 2021 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-34101467

RESUMO

Metal-organic frameworks (MOFs) offer many opportunities for applications across biology and medicine. Their wide range of chemical composition makes toxicologically acceptable formulation possible, and their high level of functionality enables possible applications as delivery systems for therapeutics agents. Surface modifications have been used in drug delivery systems to minimize their interaction with the bulk, improving their specificity as targeted carriers. Herein, we discuss a strategy to achieve a tumor-targeting drug-loaded MOF using "click" chemistry to anchor functional folic acid (FA) molecules on the surface of N3-bio-MOF-100. Using curcumin (CCM) as an anticancer drug, we observed drug loading encapsulation efficiencies (DLEs) of 24.02 and 25.64% after soaking N3-bio-MOF-100 in CCM solutions for 1 day and 3 days, respectively. The success of postsynthetic modification of FA was confirmed by 1H NMR spectroscopy, Fourier transform infrared spectroscopy (FTIR), and liquid chromatography-mass spectrometry (LC-MS). The stimuli-responsive drug release studies demonstrated an increase of CCM released under acidic microenvironments. Moreover, the cell viability assay was performed on the 4T1 (breast cancer) cell line in the presence of CCM@N3-bio-MOF-100 and CCM@N3-bio-MOF-100/FA carriers to confirm its biological compatibility. In addition, a cellular uptake study was conducted to evaluate the targeting of tumor cells.


Assuntos
Neoplasias da Mama/tratamento farmacológico , Curcumina/química , Portadores de Fármacos/química , Estruturas Metalorgânicas/química , Terapia de Alvo Molecular , Química Click , Curcumina/farmacologia , Curcumina/uso terapêutico , Ácido Fólico/química
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