Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 55
Filtrar
1.
Toxicol Ind Health ; 35(8): 507-519, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31462197

RESUMO

In commercial products such as household deodorants or biocides, didecyldimethylammonium chloride (DDAC) often serves as an antimicrobial agent, citral serves as a fragrance agent, and the excipient ethylene glycol (EG) is used to dissolve the active ingredients. The skin sensitization (SS) potentials of each of these substances are still being debated. Moreover, mixtures of DDAC or citral with EG have not been evaluated for SS potency. The in vitro alternative assay called human Cell Line Activation Test (h-CLAT) and Direct Peptide Reactivity Assay (DPRA) served to address these issues. On three independent runs of h-CLAT, DDAC and citral were predicted to be sensitizers while EG was predicted to be a non-sensitizer and also by the DPRA. Mixtures of DDAC or citral with EG at ratios of 7:3 and 1:4 w/v were all positive by the h-CLAT in terms of SS potential but SS potency was mitigated as the proportion of EG increased. Citral and its EG mixtures were all positive but DDAC and its EG mixtures were all negative by the DPRA, indicating that the DPRA method is not suitable for chemicals with pro-hapten characteristics. Since humans can be occupationally or environmentally exposed to mixtures of excipients with active ingredients, the present study may give insights into further investigations of the SS potentials of various chemical mixtures.


Assuntos
Monoterpenos Acíclicos/efeitos adversos , Etilenoglicol/efeitos adversos , Excipientes/efeitos adversos , Compostos de Amônio Quaternário/efeitos adversos , Testes de Irritação da Pele/métodos , Pele/efeitos dos fármacos , Monoterpenos Acíclicos/administração & dosagem , Alternativas aos Testes com Animais/métodos , Antígeno B7-2/metabolismo , Bioensaio/métodos , Linhagem Celular , Etilenoglicol/administração & dosagem , Excipientes/administração & dosagem , Humanos , Molécula 1 de Adesão Intercelular/metabolismo
2.
J Pharm Sci ; 108(2): 987-995, 2019 02.
Artigo em Inglês | MEDLINE | ID: mdl-30696548

RESUMO

In a previous study investigating the relationships between solute physicochemical properties and solvent effects on skin permeation of solutes under finite dose conditions, urea was found to have the highest percent dose absorbed among the model solutes studied. The objective of this study is to probe the mechanism of the observed high skin permeation of urea at finite dose, in contrast to its permeability coefficient obtained under infinite dose condition. Skin permeation experiments were performed with Franz diffusion cells and human epidermal membrane. Dose-dependence and penetration enhancing effects of urea permeation were investigated. Tape stripping was performed. A small hydrophilic solute ethylene glycol with molecular weight similar to urea was studied for comparison. The results suggest that urea did not have penetration enhancing effect to enhance solute permeation across skin under the finite dose conditions. Tape stripping data are consistent with skin permeation mechanism of solute deposition and diffusion. The skin permeation behavior of urea could be attributed to its small molecular size. This suggests that, under the finite dose conditions examined in this study, solutes with molecular sizes similar to or less than urea and ethylene glycol could lead to high percent of skin absorption despite their hydrophilicities.


Assuntos
Absorção Cutânea/efeitos dos fármacos , Ureia/farmacologia , Ureia/farmacocinética , Administração Cutânea , Idoso , Epiderme/efeitos dos fármacos , Epiderme/metabolismo , Etilenoglicol/administração & dosagem , Etilenoglicol/farmacocinética , Glicerol/administração & dosagem , Glicerol/farmacocinética , Humanos , Pessoa de Meia-Idade , Ureia/administração & dosagem
3.
Arq. bras. med. vet. zootec. (Online) ; 70(5): 1489-1496, set.-out. 2018. ilus, tab, graf
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-947122

RESUMO

The efficiency of an alternative freezing protocol for goat embryos of different morphology and quality was tested. Fifty-eight embryos on Day 6-7 stage were transferred as fresh or after freeze-thawing (n=29/group). For freezing, embryos were placed into 1.5M ethylene-glycol solution for 10min. During this time, they were loaded in the central part of 0.25mL straw, separated by air bubble from columns containing PBS/BSA 0.4% plus 20% BFS. Straws were then frozen using a freezing machine from 20ºC to -6ºC at a cooling rate of 3ºC/min, stabilization for 15min (seeding after 5min), from -6 C to -32ºC at 0.6 C/min,and plunged into liquid nitrogen. Frozen embryos were thawed for 30s at 37ºC in a water bath. Embryos subjected to fresh transfer were maintained in holding medium (37ºC). Fresh and frozen-thawed embryos were transferred at day 7 post-estrus to 30 recipients. Kidding and kid born rates were similar (P> 0.05), respectively, for recipients receiving fresh (66.7% or 10/15; 55.2% or 16/29) or frozen-thawed (60% or 9/15; 51.7% or 15/29) embryos. The cryopreservation of goat embryos using slow-freezing protocol and 1.5MEG resulted in similar efficiency rates of fresh embryos.(AU)


Este estudo testou a eficiência de protocolo alternativo de criopreservação de embriões caprinos de diferentes qualidades morfológicas. Foram utilizados 58 embriões, coletados entre o sexto e o sétimo dia do ciclo estral (n=29/grupo). Embriões congelados passaram por solução 1,5M etilenoglicol por 10min e foram aspirados durante esse tempo para parte central de palheta 0,25mL, separada por bolhas de ar de colunas contendo PBS 0,4% BSA e 20% SFB. As palhetas foram congeladas em máquina de congelação de 20ºC a -6ºC, com taxa de resfriamento de 3ºC/min, estabilização por 15min (seeding após 5min), -6ºC a -32ºC a 0,6ºC/min, e imersas em nitrogênio líquido. Os embriões foram descongelados por 30s a 37ºC, em água. Embriões frescos foram mantidos em solução de manutenção (37ºC). Embriões frescos e congelados/descongelados foram transferidos para 30 receptoras no sétimo dia do ciclo estral. A taxa de partos e a de crias nascidas (respectivamente) foram similares (P>0,05) para receptoras recebendo embriões frescos (66,7% ou 10/15; 55,2% ou 16/29) ou congelados/descongelados (60,0% ou 9/15; 51,7% ou 15/29). O protocolo de criopreservação de embriões utilizado no presente estudo resultou em índices de eficiência semelhantes aos de embriões frescos.(AU)


Assuntos
Animais , Masculino , Etilenoglicol/administração & dosagem , Ruminantes/genética , Preservação do Sêmen/estatística & dados numéricos , Agentes de Resfriamento , Transferência Embrionária/veterinária
4.
Bull Exp Biol Med ; 164(2): 207-210, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-29177872

RESUMO

We performed morphological analysis of the effect of the peptide complex from porcine kidneys on the course of experimental urolithiasis modeled in rats by treatment with 1% ethylene glycol solution (in drinking water) for 6 weeks. The peptide complex obtained by acetic acid extraction was administered in a dose of 15 mg. Administration of the peptide complex to animals with experimental kidney stone disease leads to 100% destruction of large and medium stones to the "dust" granularity.


Assuntos
Misturas Complexas/farmacologia , Rim/química , Peptídeos/farmacologia , Urolitíase/tratamento farmacológico , Animais , Misturas Complexas/química , Etilenoglicol/administração & dosagem , Masculino , Peptídeos/química , Ratos , Ratos Wistar , Suínos , Urolitíase/induzido quimicamente , Urolitíase/patologia
5.
BMJ Case Rep ; 20172017 May 27.
Artigo em Inglês | MEDLINE | ID: mdl-28551601

RESUMO

Ethylene glycol is a common alcohol found in many household products such as household hard surface cleaner, paints, varnish, auto glass cleaner and antifreeze. While extremely toxic and often fatal on ingestion, few cases with early presentation by the patient have resulted in death; thus, rapid diagnosis is paramount to effectively treating ethylene glycol poisoning. In this study, we compare two sequential cases of ethylene glycol poisoning in a single individual, which resulted in strikingly different outcomes.


Assuntos
Serviço Hospitalar de Emergência , Etilenoglicol/administração & dosagem , Etilenoglicol/envenenamento , Produtos Domésticos/envenenamento , Tentativa de Suicídio , Idoso , Antídotos/administração & dosagem , Evolução Fatal , Fomepizol , Humanos , Masculino , Insuficiência de Múltiplos Órgãos , Pirazóis/administração & dosagem , Diálise Renal , Fatores de Tempo
6.
Sci Rep ; 7: 45740, 2017 04 07.
Artigo em Inglês | MEDLINE | ID: mdl-28387228

RESUMO

TGF-ß1 is the main mediator of epithelial-to-mesenchymal transition (EMT). Hyperoxaluria induces crystalluria, interstitial fibrosis, and progressive renal failure. This study analyzed whether hyperoxaluria is associated with TGF-ß1 production and kidney fibrosis in mice and if oxalate or calcium oxalate (CaOx) could induce EMT in proximal tubule cells (HK2) and therefore contribute to the fibrotic process. Hyperoxaluria was induced by adding hydroxyproline and ethylene glycol to the mice's drinking water for up to 60 days. Renal function and oxalate and urinary crystals were evaluated. Kidney collagen production and TGF-ß1 expression were assessed. EMT was analyzed in vitro according to TGF-ß1 production, phenotypic characterization, invasion, cell migration, gene and protein expression of epithelial and mesenchymal markers. Hyperoxaluric mice showed a decrease in renal function and an increase in CaOx crystals and Ox urinary excretion. The deposition of collagen in the renal interstitium was observed. HK2 cells stimulated with Ox and CaOx exhibited a decreased expression of epithelial as well as increased expression mesenchymal markers; these cells presented mesenchymal phenotypic changes, migration, invasiveness capability and TGF-ß1 production, characterizing EMT. Treatment with BMP-7 or its overexpression in HK2 cells was effective at preventing it. This mechanism may contribute to the fibrosis observed in hyperoxaluria.


Assuntos
Oxalato de Cálcio/administração & dosagem , Transição Epitelial-Mesenquimal , Túbulos Renais Proximais/metabolismo , Túbulos Renais Proximais/patologia , Rim/lesões , Animais , Movimento Celular , Etilenoglicol/administração & dosagem , Fibrose/induzido quimicamente , Fibrose/patologia , Hidroxiprolina/administração & dosagem , Hiperoxalúria/induzido quimicamente , Túbulos Renais Proximais/efeitos dos fármacos , Camundongos Endogâmicos C57BL , Fator de Crescimento Transformador beta1/metabolismo
7.
Reprod Domest Anim ; 52 Suppl 2: 235-241, 2017 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-27862433

RESUMO

The cryopreservation of testicular tissue is presented as the only alternative for the preservation of genetic material from prepubertal animals. However, this biotechnology is still being tested. The objective of this study was to evaluate the effect of different associations of cryoprotectants and the potential of cell proliferation after vitrification of testicular tissue of prepubertal cats. Five testicular pairs from five prepubertal cats were used, and each pair was divided into four fragments. Of these, one fragment composed of the control group (CG) and the rest were distributed in experimental groups according to the associations of cryoprotectants to be tested (dimethyl sulphoxide (DMSO)/glycerol (GLY); ethylene glycol (EG)/GLY) or DMSO/EG) in a final cryoprotectant concentration of 5.6 m. The fragments were submitted to vitrification, and after one week, fragments were heated and processed for histomorphological evaluation and quantification of nucleolar organizer regions (NORs). DMSO/GLY did not differ from CG and was superior to the other vitrified groups, as to cell separation and degree of shrinkage of the basal membrane. Concerning cell differentiation, visibility of the nucleus and nuclear condensation, all the vitrified groups were inferior to CG; however, DMSO/EG was inferior to DMSO/GLY and EG/GLY, which did not differ among themselves. CG was superior to all groups in quantification of NORs. DMSO/EG was inferior to all others, and there was no difference between DMSO/GLY and EG/GLY. The association DMSO/GLY presented the best preservation of tissue integrity and potential of cell proliferation after vitrification of the testicular tissue of prepubertal cats.


Assuntos
Gatos , Criopreservação/veterinária , Crioprotetores/química , Maturidade Sexual , Testículo/fisiologia , Animais , Diferenciação Celular , Proliferação de Células , Criopreservação/métodos , Crioprotetores/administração & dosagem , Dimetil Sulfóxido/administração & dosagem , Dimetil Sulfóxido/química , Etilenoglicol/administração & dosagem , Etilenoglicol/química , Glicerol/administração & dosagem , Glicerol/química , Masculino , Testículo/citologia
8.
Sci Rep ; 6: 26326, 2016 07 14.
Artigo em Inglês | MEDLINE | ID: mdl-27412080

RESUMO

Recent studies have shown that L-proline is a natural osmoprotectant and an antioxidant to protect cells from injuries such as that caused by freezing and thawing in many species including plant, ram sperm and human endothelial cells. Nevertheless, this nontoxic cryoprotectant has not yet been applied to mammalian oocyte vitrification. In this study we evaluated the efficiency and safety of the new cryoprotectant in oocyte vitrification. The results indicated that L-proline improves the survival rate of vitrified oocytes, protects mitochondrial functions and could be applied as a new cryoprotectant in mouse oocyte vitrification.


Assuntos
Crioprotetores/farmacologia , Oócitos/efeitos dos fármacos , Prolina/farmacologia , Vitrificação/efeitos dos fármacos , Animais , Sobrevivência Celular/efeitos dos fármacos , Criopreservação/métodos , Crioprotetores/administração & dosagem , Dimetil Sulfóxido/administração & dosagem , Técnicas de Cultura Embrionária , Transferência Embrionária , Desenvolvimento Embrionário/efeitos dos fármacos , Etilenoglicol/administração & dosagem , Feminino , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos DBA , Camundongos Endogâmicos ICR , Mitocôndrias/efeitos dos fármacos , Mitocôndrias/metabolismo , Oócitos/citologia , Oócitos/metabolismo , Gravidez , Prolina/administração & dosagem , Injeções de Esperma Intracitoplásmicas , Fuso Acromático/efeitos dos fármacos , Fuso Acromático/ultraestrutura
9.
J Toxicol Sci ; 41(1): 17-24, 2016 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-26763389

RESUMO

Didecyldimethylammonium chloride (DDAC), an antimicrobial agent, has been reported to induce pulmonary toxicity in animal studies. DDAC is frequently used in spray-form household products in combination with ethylene glycol (EG). The purpose of this study was to evaluate the toxic interaction between DDAC and EG in the lung. DDAC at a sub-toxic dose (100 µg/kg body weight) was mixed with a non-toxic dose of EG (100 or 200 µg/kg body weight), and was administrated to rats via intratracheal instillation. Lactate dehydrogenase activity and total protein content in the bronchoalveolar lavage fluid (BALF) were not changed by singly treated DDAC or EG, but significantly enhanced at 1 d after treatment with the mixture, with the effect dependent on the dose of EG. Total cell count in BALF was largely increased and polymorphonuclear leukocytes were predominantly recruited to the lung in rats administrated with the mixture. Inflammatory cytokines, tumor necrosis factor-alpha and interleukin-6 also appeared to be increased by the mixture of DDAC and EG (200 µg/kg body weight) at 1 d post-exposure, which might be associated with the increase in inflammatory cells in lung. BALF protein content and inflammatory cell recruitment in the lung still remained elevated at 7 d after the administration of DDAC with the higher dose of EG. These results suggest that the combination of DDAC and EG can synergistically induce pulmonary cytotoxicity and inflammation, and EG appears to amplify the harmful effects of DDAC on the lung. Therefore pulmonary exposure to these two chemicals commonly found in commercial products can be a potential hazard to human health.


Assuntos
Anti-Infecciosos/toxicidade , Etilenoglicol/toxicidade , Produtos Domésticos/toxicidade , Pulmão/efeitos dos fármacos , Pulmão/patologia , Pneumonia/induzido quimicamente , Compostos de Amônio Quaternário/toxicidade , Animais , Relação Dose-Resposta a Droga , Combinação de Medicamentos , Interações Medicamentosas , Sinergismo Farmacológico , Etilenoglicol/administração & dosagem , Humanos , Masculino , Compostos de Amônio Quaternário/administração & dosagem , Ratos Sprague-Dawley
10.
Theriogenology ; 84(4): 545-52, 2015 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-25998270

RESUMO

This study evaluated two cryoprotectant (CPA) combinations, ethylene glycol (EG) + DMSO and EG + propylene glycol (PG), used for the vitrification of germinal vesicle (GV) porcine oocytes. In experiment 1, the equilibration of GV with the two CPA combinations increased (P < 0.05) the percentage of oocytes that degenerated after IVM (18.1 ± 2.3% and 19.4 ± 2.6% for EG + DMSO and EG + PG groups, respectively) compared with control oocytes (7.6 ± 1.3%). However, CPAs did not affect the fertilization or developmental parameters of the embryos. In experiment 2, the percentages of live vitrified-warmed GV oocytes at 2 hours after warming (EG + DMSO: 67.0 ± 2.3% and EG + PG: 57.6 ± 2.3%) were lower than those of fresh control GV oocytes (97.3 ± 0.7%). The percentage of degenerated oocytes after IVM was higher (P < 0.001) in vitrified-warmed oocytes (EG + DMSO: 59.8 ± 2.3% and EG + PG: 56.2 ± 2.6%) than in the control (1.6 ± 1.3). Fertilization efficiency was higher (P < 0.05) in the EG + PG (39.6 ± 2.4%) and control (42.0 ± 2.2%) groups than in the EG + DMSO (26.3 ± 7.7%) group. The cleavage and blastocyst formation rates of the EG + DMSO (25.9 ± 3.5% and 6.6 ± 2.5%, respectively) and EG + PG (20.2 ± 5.4% and 4.7 ± 1.6%, respectively) vitrification groups were lower (P < 0.001) than those observed in the control oocytes (53.4 ± 2.7% and 31.9 ± 1.7%, respectively). In conclusion, in the absence of vitrification, the toxic effects of both CPA combinations on the GV oocytes were minimal. Vitrification resulted in important losses in viability at each step of the in vitro embryo production procedure. However, the surviving oocytes were able to mature and be fertilized, although the fertilization efficiency in the EG + DMSO group was lower. Blastocysts formation was similar for both CPA combinations.


Assuntos
Dimetil Sulfóxido/farmacologia , Etilenoglicol/farmacologia , Polietilenoglicóis/farmacologia , Suínos/embriologia , Vitrificação/efeitos dos fármacos , Animais , Blastocisto/citologia , Blastocisto/fisiologia , Criopreservação/veterinária , Crioprotetores/farmacologia , Dimetil Sulfóxido/administração & dosagem , Técnicas de Cultura Embrionária/veterinária , Desenvolvimento Embrionário/efeitos dos fármacos , Desenvolvimento Embrionário/fisiologia , Etilenoglicol/administração & dosagem , Oócitos/efeitos dos fármacos , Oócitos/fisiologia , Polietilenoglicóis/administração & dosagem
11.
Ren Fail ; 37(4): 709-21, 2015 May.
Artigo em Inglês | MEDLINE | ID: mdl-25682972

RESUMO

BACKGROUND: Ethylene glycol (EG) exposure caused formation of calcium oxalate crystal that led to renal failure, which is associated with higher prevalence of hypertension. L-Arginine is known to have an antioxidant and nephro-protective potential. OBJECTIVE: To evaluate the effect of L-arginine against EG-induced urolithiasis in uninephrectomized hypertensive rats. MATERIAL AND METHODS: Uninephrectomized male Wistar rats (180-200 g) were used to induce urinary calculi through oral administration of EG (0.75%) in distilled water. Rats were treated with either distilled water (10 mg/kg, p.o.) or telmisartan (10 mg/kg, p.o.) or Cystone (500 mg/kg, p.o.) or L-arginine (250, 500, and 1000 mg/kg, p.o.) for 28 days. Various hemodynamic, biochemical, molecular, and histological parameters were assessed in kidney and heart. RESULTS: Rats treated with L-arginine (500 and 1000 mg/kg) significantly restored altered relative organ weight, urine output, urine density, urinary pH, and water intake. EG-induced alterations in electrocardiographic (QRS interval, HR, and ST height) and hemodynamic (SBP, DBP, MABP, and LVEDP) abnormalities were significantly restored by L-arginine (500 and 1000 mg/kg) treatment. It also significantly restored alteration in serum and urine biochemical parameters induced by EG. The elevated oxido-nitrosative stress was also significantly decreased by L-arginine (500 and 1000 mg/kg) treatment. It also significantly down-regulated EG-induced up-regulated renal KIM-1, NGAL, eNOS, and iNOs mRNA expressions. Histological aberrations induced in the renal and cardiac tissues were also ameliorated by l-arginine treatment. CONCLUSION: L-Arginine exerts its nephro- and cardio-protective potential in EG-induced urolithiasis in uninephrectomized hypertensive rats via modulation of KIM-1, NGAL, eNOS, and iNOs mRNA expression.


Assuntos
Arginina/uso terapêutico , Urolitíase/tratamento farmacológico , Proteínas da Fase Aguda/fisiologia , Animais , Moléculas de Adesão Celular/fisiologia , Etilenoglicol/administração & dosagem , Lipocalina-2 , Lipocalinas/fisiologia , Masculino , Óxido Nítrico Sintase Tipo III/fisiologia , Proteínas Proto-Oncogênicas/fisiologia , Ratos , Ratos Endogâmicos SHR , Ratos Wistar , Urolitíase/induzido quimicamente
12.
Reprod Domest Anim ; 49(5): 746-52, 2014 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-24942070

RESUMO

The objectives of this study were to compare glycerol and ethylene glycol at different concentrations as cryoprotectants and lycopene or cysteamine (with/without) as antioxidants in Tris extender for bull semen. Twenty-four ejaculates were obtained from three bulls. Each ejaculate was split into four equal aliquots and diluted using both of the Tris extenders with glycerol (5% or 7%) or ethylene glycol (3% or 5%). After that, each extenders were split into three equal aliquots and added using both of the cysteamine 5 mm or lycopene 500 µg/ml, and control (without additives). The addition of 7% glycerol with cysteamine, 5% ethylene glycol with cysteamine and 3% ethylene glycol with cysteamine groups gave the lowest CASA motility than the other groups. However, 7% glycerol and 7% glycerol with lycopene resulted in a better rate of CASA progressive motility compared with that of other groups. Generally, all the lycopene groups signed better protective effects on acrosome and total morphology than the other groups. Glycerol 7% and 3% ethylene glycol with lycopene groups yielded to slight higher percentages of membrane integrity assessed by HOST than that of the other groups, but 7% glycerol with cysteamine and 3% ethylene glycol with cysteamine showed the worst percentages of membrane integrity. Glycerol 7% and 5% glycerol with lycopene gave rise to a higher value of VAP, VSL and VCL compared with that of the other groups. On the contrary, adding to 5% glycerol with cysteamine showed negative effect for VAP, VSL, VCL and ALH values. All cryoprotectant groups with lycopene decreased chromatin damage than the other groups. Ethylene glycol 3% led to lower non-return rates of inseminated cows. However, this result was not considered to be statistically important.


Assuntos
Carotenoides/farmacologia , Bovinos/fisiologia , Crioprotetores/farmacologia , Cisteamina/farmacologia , Preservação do Sêmen/veterinária , Animais , Carotenoides/administração & dosagem , Criopreservação/métodos , Criopreservação/veterinária , Crioprotetores/administração & dosagem , Cisteamina/administração & dosagem , Etilenoglicol/administração & dosagem , Etilenoglicol/farmacologia , Fertilização In Vitro/veterinária , Glicerol/administração & dosagem , Glicerol/farmacologia , Licopeno , Masculino , Análise do Sêmen/métodos , Análise do Sêmen/veterinária , Preservação do Sêmen/métodos , Motilidade Espermática , Espermatozoides/efeitos dos fármacos
14.
Fertil Steril ; 100(1): 170-7.e1-2, 2013 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-23566598

RESUMO

OBJECTIVE: To study the preservation of follicles within ovarian tissue vitrified using only one or a combination of three permeating cryoprotectants. DESIGN: Experimental study. SETTING: University hospital. DONOR(S): Ovarian tissue was donated by consenting women undergoing elective cesarean section. INTERVENTION(S): Ovarian tissue was vitrified in closed sealed vials using either a combination of dimethyl sulfoxide, 1,2-propanediol, and ethylene glycol (EG), or only EG as permeating cryoprotectants. MAIN OUTCOME MEASURE(S): Ovarian tissue was vitrified with the use of two vitrification methods. Tissue from the same donor was used for comparison of two different solutions. The morphology of the follicles was evaluated after vitrification, warming, and culture by light microscopy and transmission electron microscopy. Apoptosis was assessed by immunohistochemistry for active caspase-3 in fresh and vitrified tissue. RESULT(S): Light and electron microscopic analysis showed equally well preserved morphology of oocytes, granulosa cells, and ovarian stroma when either of the vitrification solutions was used. No apoptosis was observed in primordial and primary follicles. CONCLUSION(S): Using only EG as a permeating cryoprotectant in a closed tube gives as good ultrastructural preservation of ovarian follicles as a more complicated system using several cryoprotectants.


Assuntos
Criopreservação/métodos , Crioprotetores/administração & dosagem , Etilenoglicol/administração & dosagem , Folículo Ovariano/efeitos dos fármacos , Folículo Ovariano/fisiologia , Vitrificação , Adulto , Sobrevivência Celular/efeitos dos fármacos , Sobrevivência Celular/fisiologia , Feminino , Humanos
15.
Eksp Klin Farmakol ; 75(3): 14-7, 2012.
Artigo em Russo | MEDLINE | ID: mdl-22679747

RESUMO

Experiments performed on 23 male rats, were divided into 2 groups. Animals in the control received group 1% solution of ethylene glycol (EG) as a drink during 6 weeks. In the test group, EG was also introduced for 6 weeks, and meloxicam was administered in a dose of 2.5 mg/kg from the 4th week. Every 7 days, daily urine was analyzed for the concentrations of oxalate, phosphate, and calcium and for the activity of urothelium injury marker enzymes includng lactate dehydrogenase (LDH), gamma-glutamyl transferase (GGT), and N-acetyl-beta-D-glucose aminidase (NAG). In addition, sections of the rats kidney were used to detect calcium deposits by histochemical Van Koss method. The treatment of experimental nephrolithiasis by meloxicame led to simplification of pathology, as indicated by a significant reduction in the urine oxalate and calcium concentrations and a pronounced decrease in the activity of all marker enzymes (LDH, GGT, NAG).This was confirmed by morphological studies, which detected very significant reduction in both number and size of calcium deposits.


Assuntos
Etilenoglicol/efeitos adversos , Rim/metabolismo , Nefrolitíase/tratamento farmacológico , Tiazinas/uso terapêutico , Tiazóis/uso terapêutico , Animais , Biomarcadores/urina , Oxalato de Cálcio/urina , Creatinina/urina , Etilenoglicol/administração & dosagem , Hexosaminidases/urina , Rim/efeitos dos fármacos , L-Lactato Desidrogenase/urina , Masculino , Meloxicam , Nefrolitíase/induzido quimicamente , Fosfatos/urina , Ratos , Tiazinas/administração & dosagem , Tiazóis/administração & dosagem , Urotélio/efeitos dos fármacos , Urotélio/enzimologia , gama-Glutamiltransferase/urina
16.
Urol Res ; 40(6): 655-61, 2012 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-22398437

RESUMO

Flos carthami (FC), also known as Carthamus tinctorius, is a traditional Chinese herbal plant that has been prescribed since centuries for treating various symptoms related to blood circulation improvement. This study aimed to investigate the effects of FC on calcium oxalate (CaOx) formation in ethylene glycol (EG)-fed rats. A total of 50 male Sprague-Dawley rats were divided into the following 6 groups: group 1, as the normal control (n = 5); group 2 received gastric gavages of starch and 0.75% EG (placebo, n = 5) as a stone inducer; group 3 (n = 10) received EG and potassium citrate as positive controls; group 4 (n = 10) received 0.75% EG and 300 mg/day FC; group 5 (n = 10) was treated with EG and 600 mg/day FC; group 6 (n = 10) received with EG and 1,200 mg/day FC. For all experimental animals, 24-h urine and blood samples were analyzed at the beginning and end of the experiment. Kidney tissue was histopathologically examined using a polarized light microscope, and crystal deposits were evaluated by a semi-quantitative scoring method; these scores were significantly lower in the FC groups (600 and 1,200 mg/day) than in the placebo group. Thus, FC administration appeared to inhibit the deposition of CaOx crystal EG-fed rats. We, therefore, consider that FC may be effective for preventing stone disease, albeit with certain side effects, such as a bleeding tendency. Further clinical trials are needed for evaluating its benefits and possible side effects.


Assuntos
Carthamus tinctorius , Fitoterapia , Extratos Vegetais/uso terapêutico , Urolitíase/prevenção & controle , Animais , Etilenoglicol/administração & dosagem , Masculino , Ratos , Ratos Sprague-Dawley , Urolitíase/induzido quimicamente
17.
Pharmacol Rep ; 64(6): 1547-53, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-23406765

RESUMO

BACKGROUND: The aim of the conducted studies was to evaluate the effect of 4-methylpyrazole, increasingly used in detoxifying treatments after ethylene glycol poisoning, on the activity of some antioxidant enzymes and lipid peroxidation formation in the liver of rats after experimental co-exposure to ethylene glycol and ethyl alcohol. METHODS: The trials were conducted on adult male Wistar rats. Ethylene glycol (EG) at the dose of 3.83 g/kg bw and ethyl alcohol (EA) at the dose of 1 g/kg bw were administered po, and 4-methylpyrazole (4-MP) at the dose of 0.01 g/kg bw was administered ip. Parameters of antioxidant balance were evaluated in hepatic cytosol, including the activity of the following enzymes: glutathione S-transferase (GST), glutathione reductase (GR), glutathione peroxidase (GPx) and lipid peroxidation level (TBARS). RESULTS: The results suggest that evaluation of the effects of administrated 4-MP after co-exposure to EG and EA in the liver revealed statistically significant changes on antioxidant enzyme system and malondialdehyde formation. CONCLUSION: The changes in biomarkers activity indicate a greater production of free radicals which exceeds the capability of antioxidant system, appearing with oxidative stress in the group of animals treated by 4-MP combined with EG and EA.


Assuntos
Antioxidantes/farmacologia , Enzimas/metabolismo , Etanol/toxicidade , Etilenoglicol/toxicidade , Peroxidação de Lipídeos/efeitos dos fármacos , Fígado/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Pirazóis/farmacologia , Administração Oral , Animais , Antioxidantes/administração & dosagem , Etanol/administração & dosagem , Etilenoglicol/administração & dosagem , Fomepizol , Glutationa Peroxidase/metabolismo , Glutationa Redutase/metabolismo , Glutationa Transferase/metabolismo , Injeções Intraperitoneais , Fígado/enzimologia , Masculino , Malondialdeído/metabolismo , Pirazóis/administração & dosagem , Ratos , Ratos Wistar , Espécies Reativas de Oxigênio/metabolismo , Substâncias Reativas com Ácido Tiobarbitúrico/metabolismo , Fatores de Tempo
18.
Urol J ; 8(3): 179-84, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-21910095

RESUMO

PURPOSE: To assess the beneficial effect of different fractions of Cynodon dactylon (C. dactylon) on ethylene glycol-induced kidney calculi in rats. MATERIALS AND METHODS: Male Wistar rats were randomly divided into control, ethylene glycol, curative, and preventive groups. The control group received tap drinking water for 35 days. Ethylene glycol, curative, and preventive groups received 1% ethylene glycol for induction of calcium oxalate (CaOx) calculus formation. Preventive and curative subjects also received different fractions of C. dactylon extract in drinking water at 12.8 mg/kg, since day 0 and day 14, respectively. After 35 days, the kidneys were removed and examined for histopathological findings and counting the CaOx deposits in 50 microscopic fields. RESULTS: In curative protocol, treatment of rats with C. dactylon N-butanol fraction and N-butanol phase remnant significantly reduced the number of the kidney CaOx deposits compared to ethylene glycol group. In preventive protocol, treatment of rats with C. dactylon ethyl acetate fraction significantly decreased the number of CaOx deposits compared to ethylene glycol group. CONCLUSION: Fractions of C. dactylon showed a beneficial effect on preventing and eliminating CaOx deposition in the rat kidney. These results provide a scientific rational for preventive and treatment roles of C. dactylon in human kidney stone disease.


Assuntos
Cynodon , Cálculos Renais/tratamento farmacológico , Fitoterapia , Extratos Vegetais/uso terapêutico , Animais , Etilenoglicol/administração & dosagem , Cálculos Renais/induzido quimicamente , Masculino , Ratos , Ratos Wistar
19.
Stat Med ; 30(15): 1825-36, 2011 Jul 10.
Artigo em Inglês | MEDLINE | ID: mdl-21495060

RESUMO

In many biomedical and epidemiological studies, data are often clustered due to longitudinal follow up or repeated sampling. While in some clustered data the cluster size is pre-determined, in others it may be correlated with the outcome of subunits, resulting in informative cluster size. When the cluster size is informative, standard statistical procedures that ignore cluster size may produce biased estimates. One attractive framework for modeling data with informative cluster size is the joint modeling approach in which a common set of random effects are shared by both the outcome and cluster size models. In addition to making distributional assumptions on the shared random effects, the joint modeling approach needs to specify the cluster size model. Questions arise as to whether the joint modeling approach is robust to misspecification of the cluster size model. In this paper, we studied both asymptotic and finite-sample characteristics of the maximum likelihood estimators in joint models when the cluster size model is misspecified. We found that using an incorrect distribution for the cluster size may induce small to moderate biases, while using a misspecified functional form for the shared random parameter in the cluster size model results in nearly unbiased estimation of outcome model parameters. We also found that there is little efficiency loss under this model misspecification. A developmental toxicity study was used to motivate the research and to demonstrate the findings.


Assuntos
Pesquisa Biomédica/métodos , Etilenoglicol/toxicidade , Organogênese/efeitos dos fármacos , Anormalidades Induzidas por Medicamentos/veterinária , Animais , Viés , Pesquisa Biomédica/estatística & dados numéricos , Análise por Conglomerados , Projetos de Pesquisa Epidemiológica , Etilenoglicol/administração & dosagem , Feminino , Seguimentos , Modelos Lineares , Camundongos , Gravidez , Resultado da Gravidez/veterinária , Tamanho da Amostra
20.
Mol Ther ; 19(5): 870-5, 2011 May.
Artigo em Inglês | MEDLINE | ID: mdl-21119625

RESUMO

Primary hyperoxaluria type I (PH1) is an inborn error of metabolism caused by deficiency of the hepatic enzyme alanine-glyoxylate aminotransferase (AGXT or AGT) which leads to overproduction of oxalate by the liver and subsequent urolithiasis and renal failure. The current therapy largely depends on liver transplantation, which is associated with significant morbidity and mortality. To explore an alternative treatment, we used somatic gene transfer in a mouse genetic model for PH1 (Agxt1KO). Recombinant adeno-associated virus (AAV) vectors containing the human AGXT complementary DNA (cDNA) were pseudotyped with capsids from either serotype 8 or 5, and delivered to the livers of Agxt1KO mice via the tail vein. Both AAV8-AGXT and AAV5-AGXT vectors were able to reduce oxaluria to normal levels. In addition, treated mice showed blunted increase of oxaluria after challenge with ethylene glycol (EG), a glyoxylate precursor. In mice, AGT enzyme activity in whole liver extracts were restored to normal without hepatic toxicity nor immunogenicity for the 50 day follow-up. In summary, this study demonstrates the correction of primary hyperoxaluria in mice treated with either AAV5 or AAV8 vectors.


Assuntos
Hiperoxalúria Primária/enzimologia , Hiperoxalúria Primária/terapia , Transaminases/metabolismo , Animais , Western Blotting , Proteínas do Capsídeo/administração & dosagem , Dependovirus/genética , Modelos Animais de Doenças , Etilenoglicol/administração & dosagem , Etilenoglicol/metabolismo , Técnicas de Transferência de Genes , Terapia Genética , Humanos , Hiperoxalúria Primária/genética , Fígado/enzimologia , Camundongos , Camundongos Knockout , Nefrocalcinose , Oxalatos/metabolismo , Fenótipo , Transaminases/deficiência , Transaminases/genética , Urolitíase
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...