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1.
Bol. latinoam. Caribe plantas med. aromát ; 18(5): 504-517, sept. 2019. ilus, tab, graf
Artigo em Inglês | LILACS | ID: biblio-1008288

RESUMO

Nowdays it is established that ischemic brain damage like ischemic stroke is one of the leading cause of death and disability in the population that assumes relevance development of anti-ischemic drugs. The work studied the anti-hypoxic and anti-ischemic effect of 7 plant extracts. Antihypoxic activity was assessed on models of hypobaric, hypercapnic, histotoxic, hematotoxic hypoxia. Anti-ischemic activity of test-extracts was studied on the focal cerebral ischemia model. Administration of Tagetes patula, Gaillardia pulchella, Sorbaria sorbifolia, Grossularia reclinata, Ribes nigrum, Rubus caesius and Lysimachia punctata extracts contributed to the necrosis zone reduction by 56.6% (p<0.05); 37.3% (p<0.05); 73.2% (p<0.05); 49.4% (p<0.05); 42.5% (p<0.05); 85.5% (p<0.05); 44.2% (p<0.05) and also restored aerobic metabolism in brain tissue. Test - objects increased of the animal lifespan under hypoxia conditions. Based on the data obtained, it is assumed that further studies of North Caucasus flora plant extracts as cerebro-protective agents are promising.


Hoy en día, se ha establecido que el daño cerebral isquémico, como el accidente cerebrovascular isquémico, es una de las principales causas de muerte y discapacidad en la población lo cual hace relevante el desarrollo fármacos antiisquémicos. En este trabajo se estudió el efecto antihipóxico y antiisquémico de siete extractos de plantas. La actividad antihipóxica se evaluó en modelos de hipoxia hipocrática, hipercápnica, histotóxica y hematotóxica. La actividad antiisquémica de los extractos de prueba se estudió en el modelo de isquemia cerebral focal. La administración de los extractos de Tagetes patula; Gaillardia pulchella; Sorbaria sorbifolia; Grossularia reclinata; Ribes nigrum; Rubus caesius y Lysimachia punctata contribuyeron a la reducción de la zona de necrosis en un 56,6% (p<0,05); 37,3% (p<0,05); 73,2% (p<0,05); 49,4% (p<0,05); 42,5% (p<0,05); 85,5% (p<0,05); 44.2% (p<0.05), respectivamente, además, de restaurar el metabolismo aeróbico en el tejido cerebral. Comparado con el control, se observó un aumento en el tiempo de sobrevida del animal en condiciones de hipoxia. Sobre la base de los interesantes datos obtenidos, se sugiere estudios adicionales de extractos de plantas de la flora del Cáucaso Norte como agentes protectores del cerebro.


Assuntos
Animais , Masculino , Camundongos , Ratos , Extratos Vegetais/uso terapêutico , Isquemia Encefálica/tratamento farmacológico , Hipóxia/tratamento farmacológico , Ensaio de Imunoadsorção Enzimática , Trifosfato de Adenosina/análise , Ratos Wistar , Ácido Láctico/análise , Ácido Pirúvico/análise , Camundongos Endogâmicos BALB C
2.
An Acad Bras Cienc ; 91(3): e20180646, 2019 Aug 12.
Artigo em Inglês | MEDLINE | ID: mdl-31411259

RESUMO

The hepatoprotective effects of the ethanolic extracts of propolis (EEP) on alcohol-induced liver steatosis were investigated in Wistar rats. Chronic alcoholic fatty liver was induced by administration of 52% alcohol to male Wistar rats at the dose of 1% body weight for 7 weeks. Then animals were simultaneously treated with 50% ethanol solutions of EEP or normal saline at the dose of 0.1% body weight for 4 further weeks. Serological analyses and liver histopathology studies were performed to investigate the development of steatosis. Microarray analysis was conducted to investigate the alterations of hepatic gene expression profiling. Our results showed that 4-week treatment of EEP helped to restore the levels of various blood indices, liver function enzymes and the histopathology of liver tissue to normal levels. Results from the microarray analysis revealed that the hepatic expressions of genes involved in lipogenesis were significantly down-regulated by EEP treatment, while the transcriptional expressions of functional genes participating in fatty acids oxidation were markedly increased. The ability of EEP to reduce the negative effects of alcohol on liver makes propolis a potential natural product for the alternative treatment of alcoholic fatty liver.


Assuntos
Fígado Gorduroso Alcoólico/metabolismo , Hepatopatias Alcoólicas/metabolismo , Extratos Vegetais/metabolismo , Própole/metabolismo , Substâncias Protetoras/metabolismo , Alanina Transaminase/metabolismo , Animais , Apiterapia/métodos , Aspartato Aminotransferases/metabolismo , Colesterol/metabolismo , Modelos Animais de Doenças , Etanol , Ácidos Graxos/biossíntese , Fígado Gorduroso Alcoólico/tratamento farmacológico , Fígado Gorduroso Alcoólico/genética , Fígado Gorduroso Alcoólico/patologia , Hepatopatias Alcoólicas/tratamento farmacológico , Hepatopatias Alcoólicas/genética , Hepatopatias Alcoólicas/patologia , Masculino , Oxirredução , Extratos Vegetais/química , Extratos Vegetais/uso terapêutico , Própole/química , Própole/uso terapêutico , Substâncias Protetoras/química , Substâncias Protetoras/uso terapêutico , Ratos Wistar , Análise Serial de Tecidos/métodos , Transcrição Genética/genética , Triglicerídeos/metabolismo
3.
Cell Physiol Biochem ; 53(2): 388-399, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31403269

RESUMO

BACKGROUND/AIMS: Doxorubicin, a chemotherapy drug used successfully for years, could induce cardiotoxicity. Euterpe oleracea Mart. (açai) is a fruit high in antioxidant properties. The aim of this study was to evaluate doxorubicin-induced cardiotoxicity prevention after açai administration. METHODS: A total of 64 male Wistar rats were allocated into 4 groups: control (C), açai (A), doxorubicin (D) and açai-doxorubicin (DA). Rats received regular chow (C and D groups) or chow supplemented with açai 5% (A and DA groups) for 4 weeks. Subsequently, rats received doxorubicin 20 mg/kg (D and DA groups) or saline (C and A groups). Euthanasia was performed 48 hours after doxorubicin injection. Left ventricular function was evaluated by echocardiography in vivo and by isolated heart study ex vivo. Oxidative stress, myocardial metabolism and nitric oxide metabolite were evaluated by spectrophotometry, MMP-2 activity by zymography and caspase-3 and Bcl-2 protein expression by Western blot. RESULTS: Doxorubicin induced decreases in body weight, food and water ingestion. We observed decreases in left ventricular fractional shortening in rats treated with doxorubicin. Additionally, the same rats showed lower +dP/dt and -dP/dt during isolated heart study than those who did not receive doxorubicin. Doxorubicin injection increased caspase-3 protein expression, myocardium lipid hydroperoxide concentration, MMP-2 activity, phosphofructokinase and lactate dehydrogenase activity, and decreased ß-hydroxyacyl-CoA dehydrogenase, pyruvate dehydrogenase, citrate synthase, complex I, complex II and ATP synthase activity in myocardium. Açai supplementation improved left ventricular fractional shortening, decreased myocardium lipid hydroperoxide concentration, MMP-2 activity, and improved ß-hydroxyacyl-CoA dehydrogenase, phosphofructokinase, citrate synthase, complex II and ATP synthase enzymatic activities. We did not observe differences in nitric oxide metabolite concentrations between groups. CONCLUSION: Doxorubicin induced left ventricular dysfunction, increases in oxidative stress, changes in myocardium metabolism and MMP-2 activation. Açai supplementation was able to prevent these alterations.


Assuntos
Doxorrubicina/toxicidade , Euterpe/química , Estresse Oxidativo/efeitos dos fármacos , Extratos Vegetais/farmacologia , Animais , Apoptose/efeitos dos fármacos , Caspase 3/metabolismo , Suplementos Nutricionais , Ecocardiografia , Euterpe/metabolismo , Cardiopatias/etiologia , Cardiopatias/prevenção & controle , Técnicas In Vitro , L-Lactato Desidrogenase/metabolismo , Masculino , Metaloproteinase 2 da Matriz/metabolismo , Miocárdio/metabolismo , Óxido Nítrico/sangue , Extratos Vegetais/química , Extratos Vegetais/uso terapêutico , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Ratos , Ratos Wistar , Função Ventricular Esquerda/efeitos dos fármacos
4.
Bol. latinoam. Caribe plantas med. aromát ; 18(4): 392-410, jul. 2019. mapas, tab, graf
Artigo em Inglês | LILACS | ID: biblio-1008179

RESUMO

An ethnobotanical study was conducted with the aim to identify the medicinal plants used for the treatment of Diabetes mellitus (DM) in Sidi Bel Abbes region (Northwest Algeria). Sidi Bel Abbes, known for its diverse ecological habitats, such as mountains, steppe, lake, and fertile plains with diverse medicinal plants. The data was collected through questionnaire and interviews with inhabitants and traditional healers. The results obtained revealed that 33 plant species distributed in 20 genera belonging to 21 families for the treatment of DM were used. The most represented families were Lamiaceae, Asteraceae, Myrtaceae, Fabaceae and Lauraceae. Medicinal plants commonly used were Trigonella foenum-graecum, Olea europaea, Cinamomum cassia, Artemisia herba-alba, Lupinus albus, Juniperus communis, Rosmarinus officinalis, Prunus dulcis and Berberis vulgaris. The study revealed that, leaves, followed by seeds and stem bark were mostly used parts. Also, decoction and infusion were the most frequently used method of preparation. This study confirms that most people with Diabetes mellitus in the study areas rely on traditional medicine for their primary health care needs.


Se realizó un estudio etnobotánico con el objetivo de identificar las plantas medicinales utilizadas para el tratamiento de la Diabetes mellitus (DM) en la región de Sidi Bel Abbes (noroeste de Algeria). Sidi Bel Abbes, conocida por sus diversos hábitats ecológicos, como montañas, estepas, lagos y fértiles llanuras con plantas medicinales. Los datos se recopilaron mediante cuestionarios y entrevistas con habitantes y curanderos tradicionales. Los resultados obtenidos revelaron que 33 especies vegetales distribuidas en 20 géneros pertenecientes à 21 familias fueron utilizadas para el tratamiento de DM. Las familias más representadas fueron Lamiaceae, Asteraceae, Myrtaceae, Fabaceae y Lauraceae. Las plantas medicinales comúnmente utilizadas fueron Trigonella foenum-graecum, Olea europaea, Cinamomum cassia, Artemisia herba-alba, Lupinus albus, Juniperus communis, Rosmarinus officinalis, Prunus amygdalus y Berberis vulgaris. El estudio reveló que las hojas, seguidas de las semillas y la corteza de tallo, eran en su mayoría partes usadas. Sin embargo, la infusión y la decocción son el método de preparación más utilizado. El estudio reveló que las hojas, seguidas de las semillas y las partes del área, eran en su mayoría partes utilizadas. Tambien, la decocción y la infusión fueron los métodos de preparación más utilizado. Este estudio confirma que la mayoría de las personas con Diabetes mellitus en las áreas d'estudio dependen de la medicina tradicional para sus necesidades d'atenciónn primaria de la salud.


Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Idoso , Plantas Medicinais , Folhas de Planta , Etnobotânica , Preparações de Plantas/uso terapêutico , Diabetes Mellitus/tratamento farmacológico , Sementes , Extratos Vegetais/uso terapêutico , Inquéritos e Questionários , Argélia , Medicina Tradicional
5.
Medicine (Baltimore) ; 98(26): e16231, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31261581

RESUMO

BACKGROUND: Crohn disease (CD) is associated with substantial healthcare related costs and impairment of quality of life. Tripterygium wilfordii Hook F (TwHF) is proved to be effective for CD in animal and human. However, there is no systemic review and meta-analysis regarding the clinical efficacy and safety of TwHF preparation for the treatment of CD. METHODS: Six electronic databases (Medline, EMBASE, Cochrane database, Chinese National Knowledge Infrastructure, Wanfang Database and Chongqing VIP Database) will be searched for eligibility studies. Data from the included studies will be extracted and the quality of studies will be assessed. Data synthesis will be performed using Review Manager software. Sensitivity analysis and publication bias assessment will also be carried out. RESULTS: This systemic review and meta-analysis will provide synthesized result of clinical efficacy and safety of TwHF preparation for the treatment of CD. CONCLUSION: This research will determine the clinical efficacy and safety of TwHF preparation for the treatment of CD.Registration: PROSPERO CRD42019127893.


Assuntos
Doença de Crohn/tratamento farmacológico , Metanálise como Assunto , Fitoterapia , Extratos Vegetais/uso terapêutico , Revisão Sistemática como Assunto , Tripterygium , Humanos , Fitoterapia/efeitos adversos , Extratos Vegetais/efeitos adversos , Projetos de Pesquisa , Resultado do Tratamento
6.
Pol Merkur Lekarski ; 46(276): 263-267, 2019 Jun 28.
Artigo em Polonês | MEDLINE | ID: mdl-31260436

RESUMO

Herbhoneys are bee origin products. However, they differ from traditional honeys in the production method. The process of making herbhoneys is based on feeding bees with nourishments, which are a combination of sugar syrup and plant extracts. For the preparation of the nourishment, plants that do not produce nectar or honeydew are often used. Thanks to this treatment, ready-made herbhoney, apart from a rich composition of mineral compounds, may also contain active substances. Herbhoneys have a characteristic color, which is associated with its plant origin and chemical composition. They are used in the therapy and prevention of many diseases, and their healthpromoting effect is associated with the plant material used to prepare the nutrient solution. Research shows positive influence of pine herbhoney in the treatment of upper respiratory tract infections, or the use of nettle herbhoney in gastrointestinal disorders. There are diverse data on the elemental composition of herbhoneys compared to natural honey. However, the essential factor conditioning the mineral content is the origin of honey and herbahoneys used for analysis. Herbhoneys, like traditional honey, have antimicrobial activity. They have been shown to be bactericidal against gram-positive and gram-negative pathogenic bacteria. The content of bioactive substances in herbhoneys determines their high antioxidant potential. Studies also indicate the presence of polyphenols, which occurrence is not observed in natural honeys. Herbhoneys, despite numerous health-promoting properties, are not a popular products on the Polish market.


Assuntos
Antioxidantes , Mel , Extratos Vegetais , Animais , Antioxidantes/uso terapêutico , Extratos Vegetais/uso terapêutico , Polônia , Polifenóis
7.
Planta Med ; 85(11-12): 997-1007, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31288278

RESUMO

Silymarin-enriched extract (SEE) is obtained from Silybum marianum (Asteraceae). Doxorubicin (DXR) is a widely used chemotherapeutical yet with severe side effects. The goal of the present study was to assess the pharmacologic effect of SEE and its bioactive components silibinin and silychristine when administrated alone or in combination with DXR in the human prostate cancer cells (PC-3). PC-3 cells were treated with SEE, silibinin (silybins A and B), silychristine, alone, and in combination with DXR, and cell proliferation was assessed by the MTT assay. Cell cycle, apoptosis, and autophagy rate were assessed by flow cytometry. Expression levels of autophagy-related genes were quantified by qRT-PCR, ELISA and western blot while transmission electron microscopy was performed to reveal autophagic structures. Finally, NMR spectrometry was used to identify specific metabolites related to autophagy. SEE inhibited PC-3 cell proliferation in a dose-dependent manner while the co-treatment (DXR-SEE) revealed an additive cytotoxic effect. Cell cycle, apoptosis, and autophagy variations were observed in addition to altered expression levels of autophagy related genes (LC3, p62, NBR1, Beclin1, ULK1, AMBRA1), while several modifications in autophagic structures were identified after DXR-SEE co-treatment. Furthermore, treated cells showed a different metabolic profile, with significant alterations in autophagy-related metabolites such as branched-chain amino acids. In conclusion, the DXR-SEE co-treatment provokes perturbations in the autophagic mechanism of prostate cancer cells (PC-3) compared to DXR treatment alone, causing an excessive cell death. These findings propose the putative use of SEE as an adjuvant cytotoxic agent.


Assuntos
Doxorrubicina/uso terapêutico , Cardo Mariano/química , Extratos Vegetais/uso terapêutico , Neoplasias da Próstata/tratamento farmacológico , Silimarina/uso terapêutico , Western Blotting , Sinergismo Farmacológico , Ensaio de Imunoadsorção Enzimática , Citometria de Fluxo , Humanos , Masculino , Células PC-3/efeitos dos fármacos , Reação em Cadeia da Polimerase em Tempo Real , Silimarina/isolamento & purificação
8.
Parasit Vectors ; 12(1): 343, 2019 Jul 12.
Artigo em Inglês | MEDLINE | ID: mdl-31300024

RESUMO

BACKGROUND: Coccidiosis represents a serious threat to the poultry industry, affecting production and causing high morbidity, mortality and significant costs resulting from treatment and prophylaxis. In-feed anticoccidials have been used for decades for managing avian coccidiosis and were very effective until drug resistance emerged. The use of natural remedies has become a promising alternative in combating coccidiosis in chickens. Therefore, the purpose of the present study was to assess the efficiency of a commercial herbal formula (H), as oral liquid preparations, in experimental chicken coccidiosis. METHODS: Two independent controlled battery experiments (BE1 and BE2) were designed and the product was tested in 3 different formulas (H1, H2 and H3): H1 contained a propylene glycol extract of Allium sativum and Thymus serpyllum; H2 contained Origanum vulgare, Satureja hortensis and Chelidonium majus; and H3 contained Allium sativum, Urtica dioica, Inula helenium, Glycyrrhiza glabra, Rosmarinus officinalis, Chelidonium majus, Thymus serpyllum, Tanacetum vulgare and Coriandrum sativum. Chickens were divided into five groups for each BE as follows: (i) uninfected untreated control (UU1, UU2); (ii) infected untreated control (IU1, IU2); (iii) infected treated with amprolium (ITA1, ITA2); and (iv, v) two experimental groups infected treated with H1 (ITH1) and H2 (ITH2) formulas in the BE1 and with H3 (ITH3-5 and ITH3-10) formula in the BE2. The chickens from infected groups were challenged with 5000 (BE1) and 50,000 (BE2) sporulated oocysts of Eimeria spp. (E. acervulina, E. tenella and E. maxima), respectively. The anticoccidial efficacy was assessed by recording the following: oocysts output (OPG), lesion score (LS), weight gain (WG), feed conversion ratio (FCR) and anticoccidial index (ACI). Additionally, polyphenolics and flavonoids (caffeic-chlorogenic acid, apigenin, kaempferol, luteolin, quercitin, quercitrin) from herb extracts found in H3 formula were determined by the liquid chromatography-tandem mass spectrometry (LC-MS/MS) method. RESULTS: H1 and H2 reduced the WG, and increased the FCR and OPG compared with controls. H1 reduced the duodenal lesions, whilst H2 reduced the caecal lesions, compared with control. H3 decreased the OPG of Eimeria spp., reduced the total lesion score and improved the zootechnical performance (weight gain and feed conversion ratio). According to ACI value, H1 and H2 had no efficacy on Eimeria spp. infection, but H3 had good to marked anticoccidial effect, the ACI being slightly greater in the group ITH3-5. According to the results of LC-MS/MS, the concentration of polyphenols in H3 formula was the highest, the sum of chlorogenic acid and caffeic acid being 914.9 µg/ml. CONCLUSIONS: H3 formula is a promising natural anticoccidial and field trials are recommended in order to validate the obtained data.


Assuntos
Coccidiose/veterinária , Coccidiostáticos/uso terapêutico , Eimeria/efeitos dos fármacos , Extratos Vegetais/uso terapêutico , Doenças das Aves Domésticas/tratamento farmacológico , Animais , Galinhas , Cromatografia Líquida , Coccidiose/tratamento farmacológico , Coccidiostáticos/química , Fezes/parasitologia , Extratos Vegetais/química , Plantas Medicinais/química , Espectrometria de Massas em Tandem , Ganho de Peso/efeitos dos fármacos
9.
Altern Ther Health Med ; 25(4): 54-63, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31202212

RESUMO

Alcohol catabolism produces oxidative stress, causing cell death and inflammation in liver tissue principally. Hawthorn (Crataegus oxyacantha) and Rosemary (Rosmarinus officinalis) are medicinal plants that have shown a potent antioxidant activity related with anti-inflammatory properties. The objective of this study was the evaluation of Hawthorn and Rosemary methanol extracts as preventive treatment in alcoholic liver disease (ALD). ALD rat model was used to measure serum hepatic enzyme levels (AST, ALT, γ-GT and ACP), total bilirubin, liver glycogen, lipid peroxidation, total antioxidant capacity (TAC) and serum lipid profile (total cholesterol, triglycerides, LDL and HDL) as well as histopathological analysis in hepatic tissues was recorder. Phytotreatments showed preventive effect, decreasing AST, γ-GT, lipid peroxidation and bilirubin indictors while TAC and liver glycogen stores increase. Interestingly, Rosemary diminished the levels of ALT and ACP. Remarkable both treatments show liver tissue damage reduction. Hawthorn proved antihyperlipidemic effect, eviting increase in all lipid indicators, while Rosemary showed antihyperlipidemic effect only in LDL levels without affecting HDL levels. The results indicate that Hawthorn and Rosemary treatments have different mechanisms of action; however they show hepatoprotective effect against ALD in rat model. Hawthorn and Rosemary could be used to prevent or help in the treatment of ALD.


Assuntos
Antioxidantes/farmacologia , Crataegus/química , Hipolipemiantes/farmacologia , Hepatopatias Alcoólicas/tratamento farmacológico , Fígado/efeitos dos fármacos , Rosmarinus/química , Animais , Antioxidantes/uso terapêutico , Hipolipemiantes/uso terapêutico , Fígado/metabolismo , Extratos Vegetais/farmacologia , Extratos Vegetais/uso terapêutico , Ratos
10.
Biomed Environ Sci ; 32(4): 291-299, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31217065

RESUMO

OBJECTIVE: Age-related diseases, including neurodegenerative diseases, are associated with oxidative stress and lipid peroxidation, and increase the levels of cholesterol auto-oxidation products such as 7ß-hydroxycholesterol (7ß-OHC). Thus, it is imperative to identify agents that can prevent 7ß-OHC-induced side-effects. METHODS: We evaluated the potential protective effects of Carpobrotus edulis ethanol-water extract (EWe) on murine oligodendrocytes (158N) cultured in the absence or presence of 7ß-OHC (20 µg/mL, 24 h). The cells were incubated with EWe (20-200 µg/mL) 2 h before 7ß-OHC treatment. Mitochondrial activity and cell growth were evaluated with the MTT assay. Photometric methods were used to analyze antioxidant enzyme [catalase (CAT) and glutathione peroxidase (GPx)] activities and the generation of lipid and protein oxidation products [malondialdehyde (MDA), conjugated diene (CD), and carbonylated proteins (CPs)]. RESULTS: Treatment with 7ß-OHC induced cell death and oxidative stress (reflected by alteration in CAT and SOD activities). Overproduction of lipid peroxidation products (MDA and CDs) and CPs was also reported. The cytotoxic effects associated with 7ß-OHC were attenuated by 160 µg/mL of EWe of C. edulis. Cell death induced by 7ß-OHC treatment was ameliorated, GPx and CAT activities were restored to normal, and MDA, CD, and CP levels were reduced following C. edulis extract treatment. CONCLUSION: These data demonstrate the protective activities of C. edulis EWe against 7ß-OHC-induced disequilibrium in the redox status of 158N cells, indicative of the potential role of this plant extract in the prevention of neurodegenerative diseases.


Assuntos
Aizoaceae , Doenças Neurodegenerativas/prevenção & controle , Oligodendroglia/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Extratos Vegetais/farmacologia , Animais , Linhagem Celular , Avaliação Pré-Clínica de Medicamentos , Hidroxicolesteróis , Camundongos , Neuroproteção , Oligodendroglia/metabolismo , Fitoterapia , Extratos Vegetais/uso terapêutico
11.
Urologiia ; (2): 15-20, 2019 Jun.
Artigo em Russo | MEDLINE | ID: mdl-31162895

RESUMO

BACKGROUND: The drug Canephron N is a combination of extracts of centaury, lovage and rosemary. Moderate antispasmoic, anti-inflammatory, antioxidant, diuretic and antimicrobial effects are of great interest for urological practice. The optimal combination of components that were made of herbal medicine allows to use their synergistic effect for prevention of recurrence of urinary stone disease. The experience of using the drug Canephron in clinical practice is of great interest. AIM: to clarify the clinical efficiency of Canephron N in patients with urinary stone disease after surgical treatment and to evaluate the changes in diuresis and calcium excretion. MATERIALS AND METHODS: The results of using the drug Canephron after surgical treatment of urinary stone disease are provided. The changes in diuresis and calcium excretion in 75 patients undergone surgical treatment of urinary stone disease were studied. Patients after ureteroscopy, percutaneous nephrolithotomy and extracorporeal shock-wave lithotripsy were prescribed treatment to prevent stone formation including herbal drug Canephron N. RESULTS: At baseline, there was negative correlation between 24-hours diuresis and calcium excretion in all groups. During follow-up, a positive correlation between 24-hours diuresis and calcium excretion was found in patients receiving Canephron N and other types of treatment. The average follow-up was 390 days. During this period, recurrence was noted in 1 patient receiving Canephron, 4 patients in patients who took other drugs and in 5 patients who didnt receive any treatment. CONCLUSION: Risk factors of stone formation persist after surgical treatment of urinary stone disease. This is reflected in a negative correlation between 24-hour diuresis and calcium excretion. During treatment, a positive correlation between diuresis and calcium excretion was noted in patients with urinary stone disease. The use of drugs that affect stone formation as well as herbal medicine Canephron N allow to obtain comparable ratio of diuresis and calcium excretion.


Assuntos
Fitoterapia , Extratos Vegetais/uso terapêutico , Cálculos Urinários/tratamento farmacológico , Cálculos Urinários/cirurgia , Cálcio/urina , Diurese/efeitos dos fármacos , Humanos , Litotripsia , Nefrolitotomia Percutânea , Extratos Vegetais/farmacologia , Prevenção Secundária , Ureteroscopia , Cálculos Urinários/prevenção & controle , Cálculos Urinários/urina
12.
Planta Med ; 85(11-12): 869-910, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31216581

RESUMO

The 2 genera Ballota and Otostegia, belonging to the Lamiaceae family, are closely related taxonomically and found mainly in the Mediterranean area, Middle East, and North Africa. Since ancient times, they have been largely employed in traditional medicine for their biological properties such as antimicrobial, anti-inflammatory, antispasmodic, insecticidal, anti-malaria, etc. Phytochemical investigations of Ballota and Otostegia species have revealed that diterpenoids are the main constituents of the genera. A large number of flavonoids and other metabolites were also identified. This review, covering literature from 1911 up to 2018, includes traditional uses, chemical profiles (both of volatile and nonvolatile metabolites), and biological properties of all the taxa of these 2 genera studied to date.


Assuntos
Ballota , Lamiaceae , Fitoterapia/métodos , Extratos Vegetais/uso terapêutico , Animais , Ballota/química , Ballota/metabolismo , Humanos , Lamiaceae/química , Lamiaceae/metabolismo , Medicina Tradicional , Extratos Vegetais/química
13.
BMC Complement Altern Med ; 19(1): 111, 2019 May 30.
Artigo em Inglês | MEDLINE | ID: mdl-31146723

RESUMO

BACKGROUND: Atherosclerosis is a condition with the vascular accumulation of lipid plaques, and its main major contributing factor is endothelial injury induced by oxidized low-density lipoprotein (ox-LDL). Salidroside (SAL) is the primary active ingredient of Rhodiola rosea, and exhibits antioxidant properties on endothelial cells and alleviates atherosclerosis. However, the effect of SAL on autophagy in ox-LDL-induced vascular endothelial injury remains unclear. Here, we investigated the effect and underlying mechanisms of SAL on autophagy in human umbilical vein endothelial cells (HUVECs). METHODS: HUVECs were incubated with ox-LDL to induce in vitro atherosclerosis model. The cell viability and injury were evaluated by cell counting kit-8 (CCK-8) assay and lactate dehydrogenase (LDH) release assay. The oxidative stress was evaluated by NADPH oxidase, malondialdehyde (MDA) and superoxide dismutase (SOD) activities. Immunofluorescence was performed to detect autophagy using LC3ß antibody. Quantitative real-time PCR (qRT-PCR) and western blot were performed to measure the mRNA expressions of SIRT1 and Forkhead box O1 (FOXO1). Nicotinamide (NAM) and AS1842856 were used to inhibit activities of SIRT1 and FOXO1, respectively. RESULTS: Exposure of HUVECs to ox-LDL (100 µg/mL) reduced cell viability, increased cellular MDA, and reduced SOD in a concentration-dependent manner. The pretreatment with SAL (20, 50 and 100 µM) significantly enhanced the cell viability and decreased LDH release in HUVECs exposed to ox-LDL (100 µg/mL). ox-LDL induced autophagy in HUVECs, which was further enhanced by pretreatment with SAL. However, SAL attenuated increase in oxidative stress in HUVECs induced by ox-LDL. ox-LDL reduced mRNA and protein expressions of SIRT1 and FOXO1, which could be reversed by SAL. The protective, anti-oxidative and pro-autophagic effects of SAL could be obviously abolished by cotreatment with SIRT1 inhibitor or FOXO1 inhibitor. CONCLUSION: Salidroside shows protective effect on endothelial cell induced by ox-LDL, and the mechanisms might be related to autophagy induction via increasing SIRT1 and FoxO1 expressions.


Assuntos
Autofagia/efeitos dos fármacos , Células Endoteliais/efeitos dos fármacos , Glucosídeos/farmacologia , Fenóis/farmacologia , Extratos Vegetais/farmacologia , Rhodiola , Aterosclerose/prevenção & controle , Avaliação Pré-Clínica de Medicamentos , Células Endoteliais/metabolismo , Proteína Forkhead Box O1/metabolismo , Glucosídeos/uso terapêutico , Células Endoteliais da Veia Umbilical Humana , Humanos , Lipoproteínas LDL , Estresse Oxidativo/efeitos dos fármacos , Fenóis/uso terapêutico , Fitoterapia , Extratos Vegetais/uso terapêutico , Sirtuína 1/metabolismo
14.
BMC Complement Altern Med ; 19(1): 117, 2019 Jun 06.
Artigo em Inglês | MEDLINE | ID: mdl-31170991

RESUMO

BACKGROUND: Glossogyne tenuifolia (GT) is a traditional herbal tea in Penghu Island, Taiwan. Its extract is traditionally been used as an antipyretic, hepatoprotective and anti-inflammatory remedy in folk medicine among local residents. The present study investigated whether GT could improve streptozotocin-induced acute liver injury of type 2 diabetes mellitus. METHODS: Male Wistar rats aged eight weeks were induced to be hyperglycemic by the subcutaneous injection of streptozotocin-nicotinamide (STZ-NA) and a combination of a high-fat diet (HFD) (N group). The animals were given GT extracts at a low dose (50 mg/kg) (L group) or a high dose (150 mg/kg) (H group) or an anti-diabetic drug (acarbose) (P group) in drinking water for 4 weeks. RESULTS: The results revealed that STZ-NA increased hepatomegaly, hepatocyte cross-sectional area, hypertrophy-related pathways (IL6/STAT3-MEK5-ERK5, NFATc3, p38 and JNK MAPK), proapoptotic molecules (cytochrome C, cleaved caspase-3), and fibrosis-related pathways (FGF-2, pERK1/2). These pathway components were then expressed at lower levels in the L and H group when compared with the N group. The liver-protective effect of GT in STZ-NA-induced diabetic rats with hyperlipidemia was through an enhancement in the activation of the compensatory PI3K-Akt and Bcl2 survival-related pathway. CONCLUSION: The results demonstrate that the hot water extracts of GT efficiently ameliorates the STZ-NA-induced diabetes associated liver damage in rat models.


Assuntos
Asteraceae , Diabetes Mellitus Experimental/complicações , Falência Hepática Aguda/prevenção & controle , Fígado/efeitos dos fármacos , Extratos Vegetais/uso terapêutico , Animais , Dieta Hiperlipídica/efeitos adversos , Avaliação Pré-Clínica de Medicamentos , Falência Hepática Aguda/etiologia , Masculino , Niacinamida , Fitoterapia , Extratos Vegetais/farmacologia , Ratos Wistar , Estreptozocina
15.
An Acad Bras Cienc ; 91(2): e20181044, 2019 Jun 19.
Artigo em Inglês | MEDLINE | ID: mdl-31241706

RESUMO

Myristica fragrans seeds are traditionally used to treat dyspepsia, vomiting and abdominal pain. The purpose of this study was to investigate the protective effect of Myristica fragrans in ethanol induced gastric ulcer. Study was carried out on rats, divided into four groups; negative control, positive control, standard drug control, and Myristica fragrans extract treated rats. The pH, ulcer index, acidity values and histopathological examination of stomach were evaluated. Myristica fragrans significantly (P<0.05) reduced gastric lesions by 41.68% in ethanol induced ulcer model at 200 mg/kg when compared to sucralfate (60.41%). However, histopathological findings appeared similar in Myristica fragrans extract treated and standard drug control groups, where stomachs were found with mild erosion of superficial epithelium and few infiltrated inflammatory cells. pH of gastric contents of rats from extract treated was increased (4.25 ± 0.25) as compared to positive control group (2.25 ± 0.25). Ulcer index of extract treated rats was improved (41.74%). Moreover, total acidity of extract treated group (60.0 ± 0.47) was decreased as compared to control group (74.50 ± 1.04). It is concluded that Myristica fragrans showed significant protecting activity in ethanol induced ulcer. Isolation and purification of phytochemicals responsible for gastroprotective activity becomes necessary.


Assuntos
Antiulcerosos/uso terapêutico , Myristica/química , Extratos Vegetais/uso terapêutico , Substâncias Protetoras/uso terapêutico , Úlcera Gástrica/tratamento farmacológico , Animais , Antiulcerosos/isolamento & purificação , Modelos Animais de Doenças , Etanol , Masculino , Extratos Vegetais/isolamento & purificação , Substâncias Protetoras/isolamento & purificação , Ratos , Ratos Wistar , Sementes , Úlcera Gástrica/induzido quimicamente , Úlcera Gástrica/patologia
16.
BMC Complement Altern Med ; 19(1): 124, 2019 Jun 10.
Artigo em Inglês | MEDLINE | ID: mdl-31182097

RESUMO

BACKGROUND: Troxerutin (TRX), a naturally occurring flavonoid in various fruits, has been reported to exhibit numerous pharmacological and biological activities in vitro and in vivo. However, the molecular mechanisms underlying TRX as a treatment for disease are poorly understood. METHODS: Using pharmacophore mapping and inverse docking, a set of potential TRX target proteins that have been associated with multiple forms of diseases was obtained. Bioinformatic analyses were performed using the Enrichr and STRING servers to analyse the related biological processes and protein-protein networks. Furthermore, we investigated the potential protective effect of TRX against lipopolysaccharide-induced acute lung injury (ALI) using a mouse model. Morphological changes in the lungs were assessed using haematoxylin and eosin staining. Inflammatory cytokines, tumour necrosis factor-α (TNF-α), interleukin-1ß (IL-1ß), IL-6 and IL-10 were investigated using ELISA. Activation of MAPK and NF-κB was detected using western blotting. RESULTS: Our network pharmacology analysis revealed the existence of multiple TRX-related chemical-target interactions and the related biological processes. We found that pretreatment with TRX protected against histological changes and obviously regulated the inflammatory cell counts and inflammatory cytokine levels in bronchoalveolar lavage fluid. Based on bioinformatic and western blot analyses, TRX may exert a protective effect against ALI by inhibiting MAPK and NF-κB signalling. CONCLUSIONS: TRX can ameliorate pulmonary injury by inhibiting the MAPK and NF-κB signalling pathways and has a potential protective effect against ALI. This study may be helpful for understanding the mechanisms underlying TRX action and for discovering new drugs from plants for the treatment of ALI.


Assuntos
Lesão Pulmonar Aguda/tratamento farmacológico , Hidroxietilrutosídeo/análogos & derivados , Lesão Pulmonar Aguda/metabolismo , Animais , Líquido da Lavagem Broncoalveolar/química , Líquido da Lavagem Broncoalveolar/imunologia , Simulação por Computador , Citocinas/metabolismo , Avaliação Pré-Clínica de Medicamentos , Ontologia Genética , Hidroxietilrutosídeo/farmacologia , Hidroxietilrutosídeo/uso terapêutico , Lipopolissacarídeos , Sistema de Sinalização das MAP Quinases , Masculino , Camundongos Endogâmicos BALB C , Terapia de Alvo Molecular , NF-kappa B/metabolismo , Fitoterapia , Extratos Vegetais/farmacologia , Extratos Vegetais/uso terapêutico , Edema Pulmonar/prevenção & controle
17.
BMC Complement Altern Med ; 19(1): 128, 2019 Jun 13.
Artigo em Inglês | MEDLINE | ID: mdl-31196061

RESUMO

BACKGROUND: Salvia officinalis has been used successfully for the treatment of hot flushes and excessive sweating during menopause. However, modes of actions have not been elucidated conclusively. We explored its pharmacology beyond any hormonal activity with a focus on neurologic impulse transmission. METHODS: A hydroalcoholic, thujone-free extract from freshly harvested Salvia officinalis leaves (A.Vogel Menosan®) was investigated in an acetylcholinesterase enzyme assay and several receptor binding assays (adrenergic alpha 2A, GABA (benzodiazepine site), GABAB; muscarinic M3, µ-opioid, serotonin 5-HT1A, serotonin 5-HT2B, serotonin 5-HT2C and serotonin transporter). The influence of the manufacturing process on additional extracts from different fresh or dry plant parts was studied. RESULTS: The Salvia officinalis extract replaced 50% of specific ligand binding to GABAA and GABAB receptors at an inhibitory concentration (IC50) of 89 and 229 µg/ml, respectively. Strong binding affinity was observed for the adrenergic α2A receptor, µ-opioid receptors, muscarinic M3 receptors, and serotonin 5-HT1A receptors, with IC50 values of 15 µg/ml, 20 µg/ml, 25 µg/ml and 19 µg/ml, respectively. Moderate interference with 5-HT2B, 5-HT2C receptors, and the human serotonin transporter was found, all with IC50 values above 32 µg/ml. Receptor binding data of Salvia extract were confirmed in native female hypothalamic tissue from two women (51 and 37 years old). Use of freshly harvested Salvia leaves resulted in 2- to 4-fold higher activity/lower IC50 values compared to extracts from dried plants or stipes. CONCLUSION: Our results suggest potent modulation of neuro-receptors and of serotonin transporters as mode of action for Salvia officinalis alcoholic extract, which may normalize thermoregulation and possibly also mental impairment during menopause.


Assuntos
Fogachos/tratamento farmacológico , Fitoterapia , Extratos Vegetais/uso terapêutico , Salvia officinalis , Transmissão Sináptica/efeitos dos fármacos , Adulto , Avaliação Pré-Clínica de Medicamentos , Feminino , Humanos , Pessoa de Meia-Idade , Extratos Vegetais/farmacologia
18.
Cell Physiol Biochem ; 53(1): 76-86, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31192545

RESUMO

BACKGROUND/AIMS: Diabetes causes damage to the enteric nervous system. The enteric nervous system consists of neurons and enteric glial cells (EGCs). The present study evaluated the effects of an ethyl-acetate fraction (EAF) from Trichilia catigua (T. catigua; 200 mg/kg) on the total population of enteric neurons (HuC/D-immunoreactive [IR]) and EGCs (S100-IR and glial fibrillary acidic protein [GFAP]-IR) in the total preparation and jejunal mucosa in diabetic rats. METHODS: The animals were distributed into four groups: normoglycemic rats (N), diabetic rats (D), normoglycemic rats that received the EAF (NC), and diabetic rats that received the EAF (DC). The jejunum was processed for immunohistochemistry to evaluate HuC/D, S100, and GFAP immunoreactivity. The expression of S100 and GFAP proteins was also quantified by Western blot. RESULTS: The D group exhibited a decrease in the number of neurons and EGCs, an increase in the area of cell bodies, an increase in S100 protein expression, a decrease in GFAP protein expression, and a decrease in S100-IR and GFAP-IR EGCs in the jejunal mucosa. The DC group exhibited a decrease in the number of neurons and EGCs, a decrease in the area of cell bodies, a decrease in S100 and GFAP protein expression, and a decrease in S100-IR and GFAP-IR EGCs in the jejunal mucosa. The NC group exhibited maintenance of the number of neurons and EGCs, an increase in the area of cell bodies, and a decrease in S100 and GFAP protein expression. CONCLUSION: The EAF from T. catigua partially conferred protection against diabetic neuropathy in the enteric nervous system.


Assuntos
Diabetes Mellitus Experimental/complicações , Neuropatias Diabéticas/prevenção & controle , Jejuno/inervação , Meliaceae/química , Neuroglia/efeitos dos fármacos , Neurônios/efeitos dos fármacos , Fármacos Neuroprotetores/farmacologia , Extratos Vegetais/farmacologia , Acetatos/química , Animais , Diabetes Mellitus Experimental/patologia , Neuropatias Diabéticas/etiologia , Neuropatias Diabéticas/patologia , Sistema Nervoso Entérico/efeitos dos fármacos , Proteína Glial Fibrilar Ácida/análise , Jejuno/efeitos dos fármacos , Jejuno/patologia , Masculino , Neuroglia/patologia , Neurônios/patologia , Neuroproteção/efeitos dos fármacos , Fármacos Neuroprotetores/química , Fármacos Neuroprotetores/uso terapêutico , Extratos Vegetais/química , Extratos Vegetais/uso terapêutico , Ratos , Ratos Wistar , Proteínas S100/análise
19.
Food Chem Toxicol ; 131: 110551, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31163217

RESUMO

Tea is a natural resource of catechins and exhibits antioxidative and anticancer activities. This study was designed to elucidate the comparative efficacy of white tea and pure EGCG in containing benzo (a) pyrene (BaP)-induced pulmonary stress. Rats were treated with white tea extract (WT) (1%) and pure EGCG at a dose of 80µg/ml in drinking water on alternate days for 12 weeks (4 weeks prior, during and after BaP treatment). BaP(50 mg/kg b. wt) was administered to rats orally in olive oil twice a week for four weeks. The indices such as stress biomarkers (LPO, PCC & ROS), antioxidant enzymes (SOD, CAT, GSH, GST, GR, GPx) activities and lung histoarchitecture were assessed. BaP administration enhanced the levels of inflammatory markers (NO and citrulline) and reduced activities of antioxidant enzymes. We observed similar antioxidant efficacy by both WT and EGCG as seen by their ameliorative action in restoring BaP induced oxidative and inflammatory stress as well as lung histoarchitecture. Our findings suggest that WT is equally beneficial as EGCG in maintaining the integrity of alveoli and is a potential candidate to be used as a cost effective and protective agent in conditions of BaP-induced lung damage.


Assuntos
Benzo(a)pireno/toxicidade , Catequina/análogos & derivados , Depuradores de Radicais Livres/uso terapêutico , Lesão Pulmonar/prevenção & controle , Extratos Vegetais/uso terapêutico , Chá/química , Animais , Biomarcadores/metabolismo , Camellia sinensis/química , Catequina/uso terapêutico , Citrulina/metabolismo , Feminino , Inflamação/metabolismo , Peroxidação de Lipídeos/efeitos dos fármacos , Pulmão/efeitos dos fármacos , Pulmão/patologia , Óxido Nítrico/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Carbonilação Proteica/efeitos dos fármacos , Ratos Sprague-Dawley , Espécies Reativas de Oxigênio/metabolismo
20.
Adv Gerontol ; 32(1-2): 121-127, 2019.
Artigo em Russo | MEDLINE | ID: mdl-31228377

RESUMO

The course intake of the nutritional supplement «Kardisten¼ normalizes increased levels of cortisol in the blood, optimizes the activity of the vessels of the brain, improving the functional state and leading to an increase in the productivity of short-term and long-term memory, more pronounced in the group of persons up to 50. At initial organic changes in the brain vessels, «Kardisten¼ significantly reduces the level of cortisol, increases to a greater extent the productivity of memory than at atherosclerosis. «Kardisten¼ activates the natural mechanisms of increasing the functional capabilities of an organism, exerting a beneficial effect on the body of adults and the elderly people, and can be recommended for the correction of physical, mental health and preservation of active longevity.


Assuntos
Transtornos da Memória , Memória , Saúde Mental , Extratos Vegetais , Adulto , Idoso , Encéfalo/efeitos dos fármacos , Humanos , Hidrocortisona , Longevidade , Transtornos da Memória/tratamento farmacológico , Extratos Vegetais/uso terapêutico
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