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1.
Braz Oral Res ; 34: e001, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32022221

RESUMO

This study analyzed the effect of prior application of copaiba oil (CO) emulsions as a dentin cleaning substance on microleakage and microtensile adhesive strength. Twenty-five premolars and sixty-four molars were used for microleakage and microtensile assays. For the microleakage assays, specimens with standard class V cavities were divided (n = 5), according to the tested CO emulsions: CO10%X, CO10%Y, and CO10%Z, as well as chlorhexidine 2% (CHX) and distilled water (DW), as positive and negative controls, respectively. Restorations were performed using the Adper Single Bond® and/or Clearfil SE Bond® systems. Cervical, occlusal, distal and mesial sections were assessed for tracer penetration degree at the composite/tooth interface. For the microtensile assay, healthy molars were divided into sixteen groups, in which artificial caries were induced in half of the groups. Dentin surfaces were treated with CO10%X and CO10%Y, CHX and DW. Microtensile bond strength was measured by fixing each sample to the plate of a universal testing machine operated at a speed of 0.5 mm/minute until failure. Dentin treated with CO10%X showed a lower infiltration rate than dentin treated with the other CO emulsions, CHX2% and DW. According to the microtensile assay, both healthy and affected dentin treated with CO10%X and Adper Single Bond® adhesive system presented higher adhesive strength. CO emulsion, used as a dentin biomodifier, interfered positively in microleakage and improved adhesive strength after acid etching in the Adper Single Bond® adhesive system, or before applying the Clearfil SE Bond® self-etching system.


Assuntos
Colagem Dentária/métodos , Adesivos Dentinários/química , Dentina/efeitos dos fármacos , Fabaceae/química , Óleos Vegetais/química , Análise de Variância , Clorexidina/química , Resinas Compostas/química , Cimentos Dentários/química , Infiltração Dentária , Dentina/química , Emulsões/química , Humanos , Teste de Materiais , Reprodutibilidade dos Testes , Cimentos de Resina/química , Estatísticas não Paramétricas , Propriedades de Superfície , Resistência à Tração
2.
Ecol Lett ; 23(2): 409-411, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31898406

RESUMO

Whether natural selection favours 'cheating' in mutualisms is hotly debated. Gano-Cohen et al. (2019a) report a negative correlation between fitness and mutualist quality in rhizobia, suggesting that rhizobia evolve to cheat. However, reanalysis of their data shows that the correlation is an artefact of unequal sampling across populations.


Assuntos
Fabaceae , Rhizobium , Seleção Genética , Simbiose
3.
Food Chem Toxicol ; 135: 110946, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31712106

RESUMO

This study investigated the inhibitory activity of serine protease, as well as antibacterial and antibiotic modifying activities of the crude extract and fractions of A. cearensis seeds. Microdilution assay was used to evaluate the antibacterial activity and the antibiotic resistance-modulating effects of samples against multiresistant bacteria Staphylococcus aureus (SA10) and Escherichia coli (EC06). In the inhibition test for serine protease, all the samples showed inhibition of enzymatic activity. Crude extract and fractions of A. cearensis seeds showed a Minimum Inhibitory Concentration ≥1024 µg/mL for all microorganisms tested. However, the samples acted as resistance modifying agent, presenting synergism when associated with gentamicin, norfloxacin and penicillin. The present study provides data indicating a possible use of the seeds extract of A. cearensis in association with antibiotics in the fight against bacterial infections.


Assuntos
Antibacterianos/farmacologia , Fabaceae/química , Extratos Vegetais/farmacologia , Inibidores de Serino Proteinase/farmacologia , Antibacterianos/isolamento & purificação , Escherichia coli/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Extratos Vegetais/isolamento & purificação , Sementes/química , Inibidores de Serino Proteinase/isolamento & purificação , Staphylococcus aureus/efeitos dos fármacos
4.
J Photochem Photobiol B ; 203: 111760, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31884350

RESUMO

An environmentally sound approach towards the green synthesis of zinc oxide nanostructures has been achieved with an aqueous extract of Calliandra haematocephala leaves. The nanoparticles were characterized using various analytical techniques to substantiate the structural details. An absorption band at 358 nm corresponds to the formation of zinc oxide nanoparticles. Scanning electron microscopy revealed the nanoflower morphology of the nanoparticles. Energy dispersive spectral analysis portrayed the strong presence of zinc and oxygen, while X-ray diffraction showed the nanoparticles to conform to hexagonally-formed wurtzite structure. The crystallite size of the nanoflowers was estimated to be 19.45 nm. Vibrational frequencies, typical of zinc­oxygen and other functional groups, were revealed using Fourier transform infrared spectroscopy. BET analysis revealed that the pores were of mesoporous nature with an estimated specific surface area of 9.18 m2/g. The photocatalytic nature of the nanoparticles was established by the degradation of methylene blue (MB) dye, under solar radiation. Up to 88% degradation was achieved in a duration of 270 min. Kinetic data from the studies proved that the reaction was compliant with first-order model, with rate constant as 0.01 min-1. The study illustrated the synthesis of zinc oxide nanoparticles using a novel source, viz., the leaves of C. haematocephala.


Assuntos
Corantes/química , Fabaceae/química , Luz , Nanopartículas Metálicas/química , Óxido de Zinco/química , Catálise , Fabaceae/metabolismo , Química Verde , Azul de Metileno/química , Oxirredução , Extratos Vegetais/química , Folhas de Planta/química , Folhas de Planta/metabolismo
5.
Gene ; 725: 144170, 2020 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-31647996

RESUMO

Caragana korshinskii Kom. is a legume shrub that is widely distributed across desert habitats with gravely, sandy, and saline soils in Asia and Africa. C. korshinskii has highly developed roots and a strong tolerance to abiotic stress. At present, there are few genetic studies of C. korshinskii because of the limited availability of genomic resources. To understand the comprehensive mechanisms that are associated with drought tolerance, we used RNA-seq to survey the differentially expressed genes (DEGs) in comparisons of drought-treated and control plants. After analysing the sequencing results, we found 440 differentially expressed genes existing in drought-treated and control plants. Among the DEGs, 39 unigenes showed up-regulated expression after drought treatment, while 401 unigenes were down-regulated. We used the KEGG database to annotate these drought-induced genes; 126 unigenes were identified by KEGG pathway annotation, and approximately 28% of the unigenes with known function fell into categories related to fatty acid metabolism, starch, sucrose metabolism, and nitrogen metabolism, suggesting that these pathways or processes may be involved in the drought response. Finally, we confirmed that one gene has a potential function in drought tolerance. Our study is the first to provide transcriptomic resources for Caragana korshinskii and to determine its digital gene expression profile under conditions of drought stress using the assembled transcriptomic data for reference. These data provide a valuable resource for genetic and genomic studies of desert plants under abiotic stress conditions.


Assuntos
Caragana/genética , Perfilação da Expressão Gênica/métodos , Estresse Fisiológico/genética , Secas , Fabaceae/genética , Regulação da Expressão Gênica de Plantas/genética , Sequenciamento de Nucleotídeos em Larga Escala/métodos , Anotação de Sequência Molecular , RNA/genética , Transcriptoma/genética
6.
Water Sci Technol ; 80(7): 1295-1303, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31850881

RESUMO

We investigated the study of the phytotoxic potential of olive mill wastewaters (OMW) on the germination and growth responses of the faba bean 'Vicia faba L.' leguminous plant cultivated under an arid Mediterranean climate. The results showed that the raw OMW blocked the germination of the seeds tested, while OMW treated soil extracts stimulate the seeds' germination rate. The monitoring of the faba bean growth parameters in the different OMW treated soils showed that the optimal growth of the faba bean plants has been recorded for the soil amended with 25 m3 ha-1 of OMW. Although the 50 m3 ha-1 dose is the most suitable for the soil studied, our results showed that the 25 m3 ha-1 dose is the most suitable for the vegetative development, as well as for the productivity of the plant tested 'Vicia faba L.'.


Assuntos
Fabaceae , Olea , Vicia faba , Germinação , Águas Residuárias
7.
Sci Total Environ ; 694: 133784, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31756809

RESUMO

Elevated atmospheric CO2 concentration (eCO2) exerts significant influence on nutrient requirement in plant. The investigation of C:N:P ratios in major cropping soils is important for managing nutrient balance and maximizing their use efficiency in future farming systems. This study aimed to examine the effect of eCO2 on the C:N:P ratios in different plant parts among soybean cultivars. Twenty-four soybean cultivars were planted in open top chambers at two CO2 concentrations (390 and 550 ppm) and sampled at the initial pod filling stage (R5) and the full maturity stage (R8). The C, N and P concentrations in root, stem, leaf and seed were determined. Elevated CO2 decreased the N concentrations in stem (-5.1%) and leaf (-3.2%) at R5, and in root (-24%), stem (-25%) and seed (-6.2%) at R8, resulting in a significant decrease of C:N ratio in the corresponding parts. The P concentration was significantly increased in root (6.0%), stem (7.9%) and leaf (16%) at R5, and in root (2.6%), stem (29%) and seed (16%) at R8 across 24 cultivars, leading to a decrease in the C:P ratio. Elevated CO2 significantly decreased the N:P ratio in root (-4.5%), stem (-12%) and leaf (-17%) at R5, and in root (-26%), stem (-57%) and seed (-22%) at R8. Furthermore, the response of C:N:P ratios to eCO2 varied greatly among soybean cultivars leading to significant CO2 × cultivar interactions. Nitrogen, but not P was the limiting factor for the soybean plants grown in Mollisols under eCO2. The considerable variation in the C:N:P ratios among cultivars in response to eCO2 indicates a potential improvement in soybean adaptability to climate change via selection new cultivars. Cultivars SN22 and ZH4 that did not considerably altered the C:N and C:P ratios in response to eCO2 are likely the optimal genomes in soybean breeding programs for eCO2 adaption.


Assuntos
Dióxido de Carbono/metabolismo , Soja/metabolismo , Biomassa , Mudança Climática , Fabaceae , Nitrogênio , Folhas de Planta , Sementes , Solo
8.
Environ Sci Technol ; 53(23): 13648-13656, 2019 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-31682413

RESUMO

Massive amounts of methyl [e.g., methylarsenate, MAs(V)] and aromatic arsenicals [e.g., roxarsone (4-hydroxy-3-nitrophenylarsonate, Rox(V)] have been utilized as herbicides for weed control and growth promotors for poultry and swine, respectively. The majority of these organoarsenicals degrade into more toxic inorganic species. Here, we demonstrate that the legume symbiont Sinorhizobium meliloti both reduces MAs(V) to MAs(III) and catalyzes sequential two-step reduction of nitro and arsenate groups in Rox(V), producing the highly toxic trivalent amino aromatic derivative 4-hydroxy-3-aminophenylarsenite (HAPA(III)). The existence of this process suggests that S. meliloti possesses the ability to transform pentavalent methyl and aromatic arsenicals into antibiotics to provide a competitive advantage over other microbes, which would be a critical process for the synthetic aromatic arsenicals to function as antimicrobial growth promoters. The activated trivalent aromatic arsenicals are degraded into less-toxic inorganic species by an MAs(III)-demethylating aerobe, suggesting that environmental aromatic arsenicals also undergo a multiple-step degradation pathway, in analogy with the previously reported demethylation pathway of the methylarsenate herbicide. We further show that an FAD-NADPH-dependent nitroreductase encoded by mdaB gene catalyzes nitroreduction of roxarsone both in vivo and in vitro. Our results demonstrate that environmental organoarsenicals trigger competition between members of microbial communities, resulting in gradual degradation of organoarsenicals and contamination by inorganic arsenic.


Assuntos
Anti-Infecciosos , Arsênico , Arsenicais , Fabaceae , Herbicidas , Roxarsona , Sinorhizobium meliloti , Animais , Antibacterianos , Suínos
9.
Zootaxa ; 4601(1): zootaxa.4601.1.1, 2019 May 07.
Artigo em Inglês | MEDLINE | ID: mdl-31717249

RESUMO

The catshark genus Scyliorhinus belongs to the family Scyliorhinidae, the most diverse family of sharks, and currently presents 16 valid species according to most modern accounts. The long history of taxonomic rearrangements and inaccurate descriptions of many species have contributed to misidentification of specimens and lack of information on the distributional range and diagnostic characters of its species. Species of Scyliorhinus are reviewed and redescribed here, with detailed descriptions on external morphology, neurocranium, claspers, dermal denticles, and tooth morphology provided for the first time for all species. Sixteen species are recognized as valid: Scyliorhinus boa (Goode Bean, 1896), S. cabofriensis Soares, Gomes de Carvalho, 2016, S. canicula (Linnaeus, 1758), S. capensis (Müller Henle, 1838), S. cervigoni Maurin Bonnet, 1970, S. comoroensis Compagno, 1988, S. duhamelii (Garman, 1913), S. garmani (Fowler, 1934), S. haeckelii (Miranda Ribeiro, 1907), S. hesperius Springer, 1966, S. meadi Springer, 1966, S. retifer (Garman, 1881), S. stellaris (Linnaeus, 1758), S. torazame (Tanaka, 1908), S. torrei Howell-Rivero, 1936, and S. ugoi Soares, Gomes Gadig, 2015. The main taxonomic decisions herein taken include the resurrection of S. duhamelii (previously a junior synonym of S. canicula) and the synonimization of S. tokubee with S. torazame. Information on geographic distribution was updated for most species, especially for those with wide ranges (S. canicula, S. haeckelii, S. retifer, and S. stellaris).


Assuntos
Fabaceae , Tubarões , Animais , Peixes , Crânio
10.
Zootaxa ; 4691(2): zootaxa.4691.2.5, 2019 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-31719404

RESUMO

Two new species of cecidomyiids were identified on Prosopis caldenia Burkart (Fabaceae) in Argentina: Rhopalomyia caldeniae Cornejo Martínez sp. nov., as the inducer of bivalve leaf galls, and the inquiline Dasineura oportunista Cornejo Martínez sp. nov. These galls are composed of the partial coalescence of two contiguous pinnulae that delimit a single larval chamber, where both species develop. Seven other species were reared from these galls as parasitoids and inquilines.


Assuntos
Dípteros , Fabaceae , Prosopis , Animais , Argentina , Tumores de Planta
11.
Zootaxa ; 4629(2): zootaxa.4629.2.8, 2019 Jul 05.
Artigo em Inglês | MEDLINE | ID: mdl-31712525

RESUMO

Two new species of Notomicrus Sharp, 1882 from Brazil are described and illustrated: N. meizon sp. nov. from the state of Amazonas and N. teramnus sp. nov. from the state of Minas Gerais. The new species can be distinguished from all other members of Notomicrus mainly by their aedeagal features, especially for N. teramnus sp. nov., with a unique aedeagal structure. In addition, new records from Brazil are provided for N. brevicornis Sharp, 1882 (states of Amazonas and Pará), N. gracilipes Sharp, 1882 (Amazonas and Rio de Janeiro), N. malkini Young, 1978 (Amazonas, Pará and Rio de Janeiro), and N. traili Sharp, 1882 (Amazonas, Goiás and Paraná).


Assuntos
Besouros , Fabaceae , Animais , Brasil
12.
Zootaxa ; 4569(1): zootaxa.4569.1.1, 2019 Mar 22.
Artigo em Inglês | MEDLINE | ID: mdl-31715841

RESUMO

The genus Neorthrius Gerstmeier Eberle, 2011 is taxonomically revised to include sixty-one species. The following thirty-one species are described as new: Neorthrius aduncus n. sp., Neorthrius aurantiacus n. sp., Neorthrius bipunctatus n. sp., Neorthrius bonasus n. sp., Neorthrius brunnorbis n. sp., Neorthrius buteocoloratus n. sp., Neorthrius cechovskyi n. sp., Neorthrius centromaculatus n. sp., Neorthrius chiangmaii n. sp., Neorthrius cornutus n. sp., Neorthrius crassopunctatus n. sp., Neorthrius ebenus n. sp., Neorthrius elegantulus n. sp., Neorthrius fortecruris n. sp., Neorthrius fulvus n. sp., Neorthrius fuscomaculosus n. sp., Neorthrius guttatus n. sp., Neorthrius longulus n. sp., Neorthrius majae n. sp., Neorthrius mariannae n. sp., Neorthrius molestus n. sp., Neorthrius schnitzeli n. sp., Neorthrius serratus n. sp., Neorthrius sexmaculatus n. sp., Neorthrius sigmoideus n. sp., Neorthrius tenuistriatus n. sp., Neorthrius tulipae n. sp., Neorthrius unicolor n. sp., Neorthrius uniformis n. sp., Neorthrius volsella n. sp. and Neorthrius zebrinus n. sp.. The following taxa, described as varietal forms, are raised to the rank of species and transferred from Orthrius Gorham, 1876: Orthrius striatopunctatus var. bimaculatus Schenkling, 1901 and Orthrius striatopunctatus var. brunneus n. syn. = Neorthrius bimaculatus (Schenkling, 1901) n. comb., n. stat.; Orthrius tarsalis var. obscurus Schenkling, 1906 = Neorthrius obscurus (Schenkling, 1906) n. comb., n. stat.; Orthrius posticalis var. nigricollis Corporaal 1926a: 180 = Neorthrius nigricollis (Corporaal, 1926), n. stat., n. comb. The following new synonymies are proposed: Orthrius deboyssyi Pic, 1951 n. syn. is synonymized with Neorthrius crassipes (Chapin, 1928) n. comb. Orthrius nigromaculatus Pic, 1951 n. syn. is synonymized with Neorthrius bimaculatus (Schenkling, 1901) n. comb. Orthrius rufitarsis Pic, 1932 n. syn., n. comb. is synonymized with Neorthrius obscurus (Schenkling, 1906). The following species are transferred to Neorthrius from Orthrius: Neorthrius angusticollis (Schenkling, 1902), n. comb., Neorthrius bengalus (Westwood, 1852) n. comb., Neorthrius bicrucis (Chapin, 1924) n. comb., Neorthrius brachialis (Gorham, 1893) n. comb., Neorthrius carinifrons (Schenkling, 1900) n. comb., Neorthrius crassipes (Chapin, 1928) n. comb., Neorthrius feae (Gorham, 1892) n. comb., Neorthrius grandjeani (Pic, 1932) n. comb.,in Neorthrius haemorrhoidalis (Schenkling, 1906) n. comb., Neorthrius innotatus (Pic, 1925) n. comb., Neorthrius madurensis (Gorham, 1895) n. comb., Neorthrius massiliensis (Pic, 1951) n. comb., Neorthrius octopunctatus (Schenkling, 1906) n. comb., Neorthrius pallidus (Chapin, 1924) n. comb., Neorthrius posticalis (Westwood, 1852) n. comb., Neorthrius pygidialis (Corporaal, 1949) n. comb., Neorthrius sexplagiatus (Schenkling, 1908) ) n. comb., Neorthrius sinensis (Gorham, 1876) n. comb., Neorthrius subfasciatus (Westwood, 1849) n. comb., Neorthrius subscalaris (Pic, 1954) n. comb., Neorthrius subsimilis (White, 1849) n. comb., Neorthrius subunicolor (Pic, 1935) n. comb., Neorthrius sulcatus (Pic, 1926) n. comb., Neorthrius sumatranus (Schenkling, 1899) n. comb., Neorthrius tarsalis (Gorham, 1892) n. comb. A key to species, color photographs of the habitus, the genitalia, the terminal abdominal segments and distribution maps are provided.


Assuntos
Besouros , Fabaceae , Animais
13.
Zootaxa ; 4674(1): zootaxa.4674.1.9, 2019 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-31716026

RESUMO

Mound and Palmer (1981) included the following eight genera in Megalurothrips genus-group: Ceratothrips, Ceratothripoides, Craspedothrips, Lefroyothrips, Megalurothrips, Odontothripiella, Odontothrips, and Projectothrips. However, a series of shared morphological characters, as well as their shared host-plant association with members of the plant family Fabaceae, indicates that Megalurothrips, Odontothrips, and Odontothripiella are particularly closely related (Xie et al. 2010). The species of these three genera all have a pair of dorso-apical setae on the first antennal segment, a character state that distinguishes them from most Thripinae (Zhang et al. 2018). Of these, Odontothrips is the most species-rich genus with 34 species distributed in the Palaearctic (ThripsWiki 2019). In Iran only 5 species (O. confusus, O. iranensis, O. loti, O. meliloti, O. phlomidinus) have been recorded (Minaei 2017). Despite more than 60 years since O. phlomidinus was described from Iran, this species remains known only from the type series (Priesner 1954). In this paper, a new species of the genus is described from southern Iran that seems to be close to O. phlomidinus. Moreover, a provisional key is provided, based on females, for six species recorded from Iran. Authority names are available on the web (ThripsWiki 2019).


Assuntos
Fabaceae , Tisanópteros , Animais , Feminino , Irã (Geográfico)
14.
Rev Col Bras Cir ; 46(5): e20192245, 2019.
Artigo em Português, Inglês | MEDLINE | ID: mdl-31778393

RESUMO

OBJECTIVE: to compare the healing by second intention under the effects of topical application of honey, copaíba oil-resin and a commercial product (fibrinolysin, deoxyribonuclease and chloramphenicol) with a control group in rats. METHODS: we carried out a skin resection, 1cm in diameter, on the back of 40 rats allocated to four groups of ten animals. All wounds were cleaned daily with 2ml of 0.9% NaCl solution. The first group (control - GC) was restricted to such procedure. In the wounds of the second (GM), third (GO) and fourth groups (GF), after cleaning, we respectively applied 1ml of honey, 1ml of copaíba oil-resin and 1ml of cream containing fibrinolysin, deoxyribonuclease and chloramphenicol. The wounds were occluded with sterile gauze. Immediately after the incision and on days three, seven and 14 of the experiment, the wounds were copied and contraction was analyzed using planimetry. After euthanasia, we histologically evaluated the inflammatory reaction and collagen in the scars. RESULTS: the reduction of the wound area of GM (p=0.003), GO (p=0.011) and GF (p=0.002) were higher than the GC. The amount of type-I collagen present in GM and GO was higher than in GC and GF groups (p<0.05). There was a predominance of chronic inflammatory stage in GM (p=0.004), GO (p<0.001) and GF (p=0.003) when compared with GC. CONCLUSION: the topical use of honey and copaíba oil-resin increases wound contraction, the presence of type-I collagen and accelerates healing.


Assuntos
Anti-Infecciosos/administração & dosagem , Fabaceae/química , Mel , Extratos Vegetais/administração & dosagem , Óleos Vegetais/administração & dosagem , Cicatrização/efeitos dos fármacos , Administração Tópica , Animais , Cloranfenicol/administração & dosagem , Desoxirribonuclease I/administração & dosagem , Modelos Animais de Doenças , Fibrinolisina/administração & dosagem , Masculino , Ratos , Ratos Wistar
15.
Zootaxa ; 4613(2): zootaxa.4613.2.9, 2019 Jun 05.
Artigo em Inglês | MEDLINE | ID: mdl-31716418

RESUMO

Two new species, Dipogon (Stigmatodipogon) siam Loktionov Lelej, sp. nov. and D. (S.) wasbaueri Loktionov Lelej, sp. nov., are described and illustrated based on females from northern Thailand and Laos. The subgenus Stigmatodipogon Ishikawa of the genus Dipogon Fox is newly recorded from Thailand. An updated key to the females is provided.


Assuntos
Fabaceae , Himenópteros , Vespas , Distribuição Animal , Animais , Feminino , Laos , Tailândia
16.
Zootaxa ; 4647(1): zootaxa.4647.1.25, 2019 Jul 26.
Artigo em Inglês | MEDLINE | ID: mdl-31716993

RESUMO

A new species of Pergalumna from soil and litter was reared in laboratory and a description of adult and juveniles is provided. Adults are characterized by having bands of striae in the posterior part of notogaster, the interlamellar seta are longer than all other prodorsal setae and the lenticular area covers most of the prodorsum. It differs from Pergalumna boliviana by having long interlamellar seta, bigger ellipsoidal porose area Aa and the presence of A2; it differs from Pergalumna paraboliviana by the shape of porose areas; i.e. Aa being ellipsoidal and big, A1 is the smallest, Ap is absent and there are longitudinal striate bands on the prodorsum and ventral plate.


Assuntos
Fabaceae , Ácaros , Animais , Laboratórios , México , Solo
17.
Zootaxa ; 4647(1): zootaxa.4647.1.26, 2019 Jul 26.
Artigo em Inglês | MEDLINE | ID: mdl-31716994

RESUMO

A new species, Pergalumna (Pergalumna) sidorchukae sp. nov., and two newly recorded species, Pergalumna (Pergalumna) jongkyui and Pergalumna (Pergalumna) amorpha, of oribatid mites of the subgenus Pergalumna (Pergalumna) from China, are described and illustrated in this study. The new species is similar to Pergalumna (Pergalumna) yurtaevi, but it differs from the latter by the surface of notogaster, the shape and size of notogastral porose areas, and the absence of postanal porose area.


Assuntos
Fabaceae , Ácaros , Animais , China
18.
Arq Bras Cir Dig ; 32(3): e1451, 2019.
Artigo em Inglês, Português | MEDLINE | ID: mdl-31644671

RESUMO

BACKGROUND: Hypovolemic shock is a common disease in polytrauma patients and may develop ischemia in various organs, increasing morbidity and mortality. The bowel is usually most affected by this condition. AIM: To evaluate the effects of copaiba oil on the intestinal mucosa's injury of rats submitted to hypovolemic shock. METHOD: Fifteen rats were divided into three groups: sham - simulated surgery; ischemia - animals submitted to hypovolemic shock; and copaiba - animals submitted to hypovolemic shock previously treated with copaiba oil. Mean blood pressure, arterial blood gas after shock induction, degree of intestinal lesion and villus length were evaluated. RESULTS: The sham presented the lowest values of lactate and PaCO2 and the highest values of mean arterial pressure, pH and bicarbonate in relation to the other groups. The degree of mesenteric lesion was zero in the sham group; 3.00±1.00 in the ischemia group; and 3.00±0.71 in the copaiba group. The villus length was 173.60±8.42 in the sham, 142.77±8.33 in the ischemia and 143.01±9.57 in the copaiba group. There was a significant difference between the sham and the other groups (p<0.05); however, there not significant difference between groups Ischemia and copaiba. CONCLUSION: Administration of copaiba oil did not reduce the intestinal mucosa lesion of rats after hypovolemic shock.


Assuntos
Anti-Inflamatórios/farmacologia , Fabaceae/química , Mucosa Intestinal/efeitos dos fármacos , Óleos Vegetais/farmacologia , Choque , Animais , Anti-Inflamatórios/química , Anti-Inflamatórios/uso terapêutico , Modelos Animais de Doenças , Íleo/patologia , Mucosa Intestinal/patologia , Isquemia/tratamento farmacológico , Masculino , Óleos Vegetais/química , Óleos Vegetais/uso terapêutico , Distribuição Aleatória , Ratos Wistar , Choque/tratamento farmacológico
19.
Int J Syst Evol Microbiol ; 69(12): 3955-3960, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31665096

RESUMO

A yellow-pigmented bacterial strain, designated T13T, was isolated from the rhizosphere soil of Alhagi sparsifolia collected from Xinjiang, PR China. Cells were rod-shaped, Gram-stain-negative, aerobic and gliding. Strain T13T grew optimally at 25-30 °C and pH 7.0-8.0 with a NaCl tolerance of 0-2 % on Reasoner's 2A agar. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain T13T belonged to the genus Flavobacterium within the family Flavobacteriaceae and was closely related to Flavobacterium nitrogenifigens KCTC 42884T with a similarity value of 97.4 %. The major polar lipid was phosphatidylethanolamine; the only respiratory quinone was MK-6, and the polyamine profile contained sym-homospermidine as the major polyamine and a trace amount of spermidine. The major fatty acids were iso-C15 : 0, C16 :  1ω7c and summed feature 3 (comprising C16 : 1ω7c or C16 : 1ω6c). The G+C content of the genomic DNA was 34.1 mol%. It is concluded from the phenotypic and genotypic data that strain T13T represents a novel species of the genus Flavobacterium, for which the name Flavobacteriumustbae sp. nov. with the type strain T13T (=KCTC 62874T=ACCC 60126T) is proposed.


Assuntos
Fabaceae/microbiologia , Flavobacterium/classificação , Filogenia , Rizosfera , Microbiologia do Solo , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/química , Flavobacterium/isolamento & purificação , Fosfatidiletanolaminas/química , Fosfolipídeos/química , Pigmentação , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Espermidina/análogos & derivados , Espermidina/química , Vitamina K 2/análogos & derivados , Vitamina K 2/química
20.
J Anim Sci ; 97(11): 4625-4634, 2019 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-31588955

RESUMO

Livestock production systems are an essential agribusiness activity in Brazil, but a critical challenge of Brazilian farmers is to maintain the equilibrium of the ecosystem, using herbage resources efficiently with a minimum impact on the environment. Nitrogen (N) fertilization and the inclusion of forage legumes into tropical grass pastures are management strategies which increase the productivity and nutritive value of pastures and may also affect methane (CH4) production by ruminants. The objective of this study was to examine the effects of either fertilizing palisade grass pastures with N or including the forage peanut (Arachis pintoi) into grass pastures on enteric CH4 emission, microbial protein production in the rumen via purine derivatives in the urine, and N balance. Twenty-one nonlactating crossbred dairy heifers were used in a completely randomized design with 3 treatments. The treatments consisted of pastures of palisade grass without N fertilization (control), fertilized with urea (fertilized), and palisade grass mixed with forage peanut (mixed). Seven animals (replications) were used to evaluate dry matter intake, digestibility, CH4 emission, urea, purine derivatives, and volume of urine, and N ingestion and excretion. Four paddocks (replications) were used to measure herbage mass; morphological, botanical, and chemical composition of herbage; and herbage allowance. The CH4 emissions were determined using the sulfur hexafluoride (SF6) tracer gas technique. The efficiency of N utilization (ENU) was calculated using the N balance data. Crude protein (CP) concentration of herbage increased with fertilization or legumes inclusion (P < 0.0001) while neutral detergent fiber (NDF) concentration decreased (P = 0.0355). The leaf allowance was higher in the fertilized treatment (P = 0.0294). Only uric acid excretion increased with N fertilization (P = 0.0204). The ENU was not affected by fertilized or mixed compared to control and averaged 55% (P = 0.8945). The enteric CH4 production was similar between treatments and averaged 129 g/d (P = 0.3989). We concluded that the changes in chemical composition of herbage provided by N fertilization or the inclusion of the legume showed no reduction in enteric CH4 emissions, but the ENU was more significant than previous studies with palisade grass, suggesting that different management strategies might alter the ENU under grazing conditions.


Assuntos
Ração Animal/análise , Arachis , Bovinos/fisiologia , Metano/metabolismo , Nitrogênio/metabolismo , Poaceae , Animais , Brasil , Indústria de Laticínios , Dieta/veterinária , Fibras na Dieta/metabolismo , Ecossistema , Fabaceae , Feminino , Fertilizantes , Valor Nutritivo , Rúmen/metabolismo , Estações do Ano
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