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1.
Zhongguo Zhong Yao Za Zhi ; 45(6): 1384-1392, 2020 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-32281352

RESUMO

The chemical compounds in water extract of Spatholobi Caulis were further studied. The compounds were systematically isolated and purified by using various separation and analysis techniques including silica gel, macroporous adsorptive resins and Sephadex LH-20 column chromatographies, as well as reversed phase high-performance liquid chromatography(RP-HPLC). Twenty-three flavonoids and one chromone were identified by the spectroscopic analysis techniques combining their physicochemical properties, they were identified as isoduartin(1), sativan(2), 8-O-methylretusin(3), 7-hydroxydihydroflavone(4), odoratin(5), butesuperin A(6), biochanin A(7), 3'-methoxydaidzein(8), 7-hydroxychromone(9), calycosin(10), naringenin(11), dihydrocajanin(12),(6 aR,11 aR)-maackiain(13), 2'-hydroxygenistein(14),(6 aR,11 aR)-medicarpin-3-O-glucopyranoside(15),(-)-epiafzelechin(16),(-)-catechin(17),(-)-epicatechin(18), 4',8-dimethoxy-7-O-ß-D-glucopyranosylisoflavone(19), ononin(20),(-)-gallocatechin(21), rutin(22), daidzin(23) and sphaerobioside(24). Compounds 4, 6, 8, 9, 11, 12, 14-16, 19 and 22-24 were isolated from Spatholobi Caulis for the first time.


Assuntos
Medicamentos de Ervas Chinesas/química , Fabaceae/química , Flavonoides/química , Compostos Fitoquímicos/química , Cromatografia Líquida de Alta Pressão , Flavonoides/isolamento & purificação , Compostos Fitoquímicos/isolamento & purificação
2.
Int J Mol Sci ; 21(7)2020 Mar 25.
Artigo em Inglês | MEDLINE | ID: mdl-32218156

RESUMO

This study examined the biological activities of copaiba essential oil via measurement of its effects on signaling pathways in the SH-SY5Y neuronal cell line. Nanofluidic proteomic technologies were deployed to measure the phosphorylation of biomarker proteins within the signaling cascades. Interestingly, copaiba essential oil upregulated the pI3K/Akt/mTOR, MAPK, and JAK/STAT signaling pathways in neuronal cells. The effects of copaiba essential oil peaked at 30 min post-treatment, with a half-maximal effective concentration (EC50) of approximately 80 ng/mL. Treatment with cannabinoid receptor 2 (CB2) agonist AM1241 or the inverse agonist BML190 abrogated the regulatory effects of copaiba essential oil on the pI3K/Akt/mTOR signaling pathway. Surprisingly, copaiba essential oil also activated the apoptosis signaling pathway and reduced the viability of SH-SY5Y cells with an EC50 of approximately 400 ng/mL. Furthermore, ß-caryophyllene, a principal constituent of copaiba essential oil, downregulated the pI3K/Akt/mTOR signaling pathway. Taken together, the findings indicated that copaiba essential oil upregulated signaling pathways associated with cell metabolism, growth, immunity, and apoptosis. The biological activities of copaiba essential oil were determined to be fast acting, CB2 mediated, and dependent on multiple chemical constituents of the oil. Nanofluidic proteomics provided a powerful means to assess the biological activities of copaiba essential oil.


Assuntos
Fabaceae/química , Neuroblastoma/metabolismo , Óleos Voláteis/farmacologia , Óleos Vegetais/farmacologia , Transdução de Sinais/efeitos dos fármacos , Linhagem Celular Tumoral , Proteínas Quinases Dependentes de AMP Cíclico/metabolismo , Humanos , Neuroblastoma/patologia , Fosfatidilinositol 3-Quinases/metabolismo , Serina-Treonina Quinases TOR/metabolismo
3.
J Agric Food Chem ; 68(10): 3238-3249, 2020 Mar 11.
Artigo em Inglês | MEDLINE | ID: mdl-32059106

RESUMO

Enrichment of multiple bioactive components with different characters into one food substrate simultaneously is a challenge. In this study, the hydrophilic epigallocatechin gallate (EGCG) and the hydrophobic quercetin were simultaneously enriched in the cavity of phytoferritin from red bean seed deprived of iron (apoRBF), a cagelike protein. The interactions of apoRBF with EGCG and quercetin were evaluated by UV/visible absorption, fluorescence, and circular dichroism technologies. By combination of the reversible assembly and urea induced approaches, both EGCG and quercetin were successfully coencapsulated in apoRBF to fabricate four kinds of apoRBF-EGCG-quercetin nanocomplexes FEQ (FEQ1, FEQ2, FEQ3, and FEQ4) with good solubility in aqueous solution. All FEQ samples maintained the typically spherical morphology of ferritin cage with a diameter around 12 nm. Among the four FEQ samples, the FEQ1 prepared by involving a pH 2.0/6.7 transition scheme was more effective in encapsulating EGCG and quercetin molecules than that by the urea induced method. Furthermore, all FEQs facilitated the stability of EGCG and quercetin molecules relative to free ones, and simultaneous coencapsulation of EGCG and quercetin could significantly improve the quercetin stability as compared with that of the free one and quercetin-loaded ferritin (p < 0.05), respectively. This work provides a new scheme to design and fabricate the ferritin based carrier for encapsulation of multiple bioactive components, and it is beneficial for the intensification of multifunction in one food substrate.


Assuntos
Catequina/análogos & derivados , Ferritinas/química , Proteínas de Plantas/química , Catequina/química , Portadores de Fármacos/química , Composição de Medicamentos , Estabilidade de Medicamentos , Fabaceae/química , Concentração de Íons de Hidrogênio , Interações Hidrofóbicas e Hidrofílicas , Sementes/química
4.
Braz Oral Res ; 34: e001, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32022221

RESUMO

This study analyzed the effect of prior application of copaiba oil (CO) emulsions as a dentin cleaning substance on microleakage and microtensile adhesive strength. Twenty-five premolars and sixty-four molars were used for microleakage and microtensile assays. For the microleakage assays, specimens with standard class V cavities were divided (n = 5), according to the tested CO emulsions: CO10%X, CO10%Y, and CO10%Z, as well as chlorhexidine 2% (CHX) and distilled water (DW), as positive and negative controls, respectively. Restorations were performed using the Adper Single Bond® and/or Clearfil SE Bond® systems. Cervical, occlusal, distal and mesial sections were assessed for tracer penetration degree at the composite/tooth interface. For the microtensile assay, healthy molars were divided into sixteen groups, in which artificial caries were induced in half of the groups. Dentin surfaces were treated with CO10%X and CO10%Y, CHX and DW. Microtensile bond strength was measured by fixing each sample to the plate of a universal testing machine operated at a speed of 0.5 mm/minute until failure. Dentin treated with CO10%X showed a lower infiltration rate than dentin treated with the other CO emulsions, CHX2% and DW. According to the microtensile assay, both healthy and affected dentin treated with CO10%X and Adper Single Bond® adhesive system presented higher adhesive strength. CO emulsion, used as a dentin biomodifier, interfered positively in microleakage and improved adhesive strength after acid etching in the Adper Single Bond® adhesive system, or before applying the Clearfil SE Bond® self-etching system.


Assuntos
Colagem Dentária/métodos , Adesivos Dentinários/química , Dentina/efeitos dos fármacos , Fabaceae/química , Óleos Vegetais/química , Análise de Variância , Clorexidina/química , Resinas Compostas/química , Cimentos Dentários/química , Infiltração Dentária , Dentina/química , Emulsões/química , Humanos , Teste de Materiais , Reprodutibilidade dos Testes , Cimentos de Resina/química , Estatísticas não Paramétricas , Propriedades de Superfície , Resistência à Tração
5.
Prep Biochem Biotechnol ; 50(1): 28-36, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31453734

RESUMO

Inga edulis is a fruit tree native from Central and South America. Different species of Inga have demonstrated high polyphenolic content and high antioxidant capacity. The oxidative stress significantly contributes to the development of several chronicle diseases, particularly age-related diseases. Therefore, photochemoprevention is an emerging interest in the use of naturally occurring antioxidants for their therapeutic use. A partial purification of the extract was carried out onto macroporous resin and the main components of I. edulis leaf extract and fraction were identified using UPLC-DAD-MS/MS as epicatechin, apigenin C-di-hexoside, myricetin-O-hexose-deoxyhexose, myricetin-O-deoxyhexose and vicenin-2, which was identified for the first time in I. edulis. Both extract and fraction presented high antioxidant capacity, the fraction exhibiting higher polyphenol and flavonoid contents and higher content of vicenin-2, suggesting that the fractioning process effectively resulted in the partial purification of the extract. The cytotoxicity and photostability were assessed in L929 fibroblast cells to evaluate whether I. edulis extract and fraction were suitable for topical application. The UVA and UVB irradiated extract and fraction did not decrease the antioxidant capacity and cell viability of L929 fibroblasts, demonstrating the photostability of the samples. The fractioning process successfully purified and enriched I. edulis extract, and both the extract and fraction are potential candidates to be safely incorporated in topical photochemopreventive formulations.


Assuntos
Antioxidantes/química , Antioxidantes/farmacologia , Fabaceae/química , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Animais , Antioxidantes/isolamento & purificação , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Cromatografia Líquida de Alta Pressão , Frutas/química , Peroxidação de Lipídeos/efeitos dos fármacos , Camundongos , Extratos Vegetais/isolamento & purificação , Espectrometria de Massas em Tandem
6.
J Chromatogr Sci ; 57(10): 944-949, 2020 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-31674640

RESUMO

One of the major problems with the formulation of herbal medicines is the quality control of plant material to ensure its efficacy and safety. Quality control of medicinal plants requires analysis of many bioactive compounds present in the plant. C-alkylated flavonoids are an important bioactive subclass of flavonoids. A simple, rapid, sensitive and selective method is presented here for the quantification of bioactive C-alkylated flavonoids. This is the first quantitative method for analysis of C-alkylated flavonoids based on the multiple reaction monitoring (MRM) approach so far. This study focuses on method development for quantification of bioactive C-alkylated flavonoids. Quantification of a total of five C-alkylated flavonoids was done employing the MRM approach on an HPLC-QqQ-MS instrument. LODs and LOQs for quantified flavonoids were in the range of 0.41-1.32 and 1.23-3.96 ng/mL, respectively. Linear calibration curves between 25 and 1500 ng/mL were obtained with the regression coefficients of ≥0.996. Accuracy (% bias) and precision (% RSD) of the analyses were found to be less than 5%. Developed HPLC-ESI-MS/MS can be employed as a quality control method of plant raw materials.


Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Fabaceae/química , Flavonoides/análise , Extratos Vegetais/química , Espectrometria de Massas em Tandem/métodos , Limite de Detecção , Modelos Lineares , Modelos Químicos , Reprodutibilidade dos Testes
7.
Chem Biodivers ; 17(1): e1900440, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31661599

RESUMO

Two analytical methods were developed in this study for direct and fast chemical investigation of authentic Copaifera oleoresins (COR) and commercial products. Polydimethylsiloxane microfiber coupled to gas chromatography-mass spectrometry (HS-SPME-GC/MS) showed the best results for oleoresin qualitative analysis, setting the following extraction conditions: equilibrium time of 15 min, extraction time of 30 min, extraction temperature at 60 °C and constant stirring of 400 rpm. Sesquiterpenes α-copaene, ß-elemene, ß-caryophyllene and trans-α-bergamotene were found in all investigated samples. Quantitative analysis by gas chromatography coupled with flame ionization detector (GC-FID) measured the content of the four sesquiterpenes in all samples. Qualitative and quantitative results showed important differences between COR of distinct species and commercial products. Data regarding the volatile composition of C. oblongifolia and C. trapezifolia oleoresins were first presented in this study and two new analytical methods were reported for direct and fast qualitative and quantitative analysis of COR.


Assuntos
Fabaceae/química , Compostos Orgânicos Voláteis/análise , Cromatografia Gasosa-Espectrometria de Massas , Microextração em Fase Sólida
8.
Food Chem Toxicol ; 135: 110946, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31712106

RESUMO

This study investigated the inhibitory activity of serine protease, as well as antibacterial and antibiotic modifying activities of the crude extract and fractions of A. cearensis seeds. Microdilution assay was used to evaluate the antibacterial activity and the antibiotic resistance-modulating effects of samples against multiresistant bacteria Staphylococcus aureus (SA10) and Escherichia coli (EC06). In the inhibition test for serine protease, all the samples showed inhibition of enzymatic activity. Crude extract and fractions of A. cearensis seeds showed a Minimum Inhibitory Concentration ≥1024 µg/mL for all microorganisms tested. However, the samples acted as resistance modifying agent, presenting synergism when associated with gentamicin, norfloxacin and penicillin. The present study provides data indicating a possible use of the seeds extract of A. cearensis in association with antibiotics in the fight against bacterial infections.


Assuntos
Antibacterianos/farmacologia , Fabaceae/química , Extratos Vegetais/farmacologia , Inibidores de Serino Proteinase/farmacologia , Antibacterianos/isolamento & purificação , Escherichia coli/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Extratos Vegetais/isolamento & purificação , Sementes/química , Inibidores de Serino Proteinase/isolamento & purificação , Staphylococcus aureus/efeitos dos fármacos
9.
J Photochem Photobiol B ; 203: 111760, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31884350

RESUMO

An environmentally sound approach towards the green synthesis of zinc oxide nanostructures has been achieved with an aqueous extract of Calliandra haematocephala leaves. The nanoparticles were characterized using various analytical techniques to substantiate the structural details. An absorption band at 358 nm corresponds to the formation of zinc oxide nanoparticles. Scanning electron microscopy revealed the nanoflower morphology of the nanoparticles. Energy dispersive spectral analysis portrayed the strong presence of zinc and oxygen, while X-ray diffraction showed the nanoparticles to conform to hexagonally-formed wurtzite structure. The crystallite size of the nanoflowers was estimated to be 19.45 nm. Vibrational frequencies, typical of zinc­oxygen and other functional groups, were revealed using Fourier transform infrared spectroscopy. BET analysis revealed that the pores were of mesoporous nature with an estimated specific surface area of 9.18 m2/g. The photocatalytic nature of the nanoparticles was established by the degradation of methylene blue (MB) dye, under solar radiation. Up to 88% degradation was achieved in a duration of 270 min. Kinetic data from the studies proved that the reaction was compliant with first-order model, with rate constant as 0.01 min-1. The study illustrated the synthesis of zinc oxide nanoparticles using a novel source, viz., the leaves of C. haematocephala.


Assuntos
Corantes/química , Fabaceae/química , Luz , Nanopartículas Metálicas/química , Óxido de Zinco/química , Catálise , Fabaceae/metabolismo , Química Verde , Azul de Metileno/química , Oxirredução , Extratos Vegetais/química , Folhas de Planta/química , Folhas de Planta/metabolismo
10.
Ultrason Sonochem ; 61: 104809, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31670252

RESUMO

Phenolic compounds, obtained from plants are important in the food, biomaterial and pharmaceutical industries; however current extraction methods, such as Soxhlet (solid-liquid) extraction, liquid-liquid extraction, microwave-assisted extraction, and ultrasonic extraction (USE), have the disadvantages of large processing times, contamination by solvents, and degradation of analytes. This study demonstrates that shock wave-assisted extraction can be used as a more efficient, eco-friendly and rapid method. Extraction of powdered samples of Eysenhardtia polystachia heartwood, a plant with high concentration of phenolic compounds, exposed to different doses of underwater shock waves, was compared with the conventional methods. Our results revealed that shock wave-assisted extraction (1500 shock waves with a peak positive pressure of approximately 88 MPa) produced 34.54% and 31.95% higher contents than Soxhlet and USE, respectively. Extraction times using shock waves were much shorter than with all other methods tested, proving that it is an attractive method to obtain both phenolic acids and flavonoids without the need for organic solvents. Furthermore, shock waves produced a significantly higher content of total reducing sugars than Soxhlet extraction and less phenolic acids which gives the insight of a more selective extraction of components.


Assuntos
Fabaceae/química , Flavonoides/isolamento & purificação , Ondas Ultrassônicas , Hidroxibenzoatos/isolamento & purificação , Extração Líquido-Líquido , Extratos Vegetais/química
11.
J Food Sci ; 85(2): 404-413, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31887250

RESUMO

Determining the impacts of extrusion conditions on extrudate characteristics of whole beans flours is critical to find the suitable types of beans to use for making direct expanded products. Whole bean flours of four different bean types, faba, lima, pinto, and red kidney, were extruded. The influence of barrel temperature (120, 140, and 160 °C), moisture content (17%, 21%, and 25%), and screw speed (150, 200, and 250 rpm) on process and product responses was studied with a corotating twin screw extruder. The barrel temperature, moisture content, screw speed, and variety of bean had significant influence on process and product responses, back pressure (MPa), torque (N·m), specific mechanical energy (kJ/kg), expansion ratio, water absorption index (g/g), and water solubility index (%) (P < 0.05). Faba bean extrudates had a significantly higher expansion ratio compared to other beans (lima, pinto, and red kidney beans) even though faba bean contained significantly higher protein and higher crude fiber contents (P < 0.05). PRACTICAL APPLICATION: The outputs of this research will be helpful to the food industry in the production of high nutrient-dense food products from whole beans by maintaining the expansion and texture of the products. The data should assist to choose the suitable types of whole bean flours and the optimum processing conditions for making direct expanded extruded products.


Assuntos
Fabaceae/química , Farinha/análise , Preparações de Plantas/isolamento & purificação , Vicia faba/química , Manipulação de Alimentos , Indústria Alimentícia , Preparações de Plantas/química , Solubilidade , Temperatura , Água/análise
12.
Rev Col Bras Cir ; 46(5): e20192245, 2019.
Artigo em Português, Inglês | MEDLINE | ID: mdl-31778393

RESUMO

OBJECTIVE: to compare the healing by second intention under the effects of topical application of honey, copaíba oil-resin and a commercial product (fibrinolysin, deoxyribonuclease and chloramphenicol) with a control group in rats. METHODS: we carried out a skin resection, 1cm in diameter, on the back of 40 rats allocated to four groups of ten animals. All wounds were cleaned daily with 2ml of 0.9% NaCl solution. The first group (control - GC) was restricted to such procedure. In the wounds of the second (GM), third (GO) and fourth groups (GF), after cleaning, we respectively applied 1ml of honey, 1ml of copaíba oil-resin and 1ml of cream containing fibrinolysin, deoxyribonuclease and chloramphenicol. The wounds were occluded with sterile gauze. Immediately after the incision and on days three, seven and 14 of the experiment, the wounds were copied and contraction was analyzed using planimetry. After euthanasia, we histologically evaluated the inflammatory reaction and collagen in the scars. RESULTS: the reduction of the wound area of GM (p=0.003), GO (p=0.011) and GF (p=0.002) were higher than the GC. The amount of type-I collagen present in GM and GO was higher than in GC and GF groups (p<0.05). There was a predominance of chronic inflammatory stage in GM (p=0.004), GO (p<0.001) and GF (p=0.003) when compared with GC. CONCLUSION: the topical use of honey and copaíba oil-resin increases wound contraction, the presence of type-I collagen and accelerates healing.


Assuntos
Anti-Infecciosos/administração & dosagem , Fabaceae/química , Mel , Extratos Vegetais/administração & dosagem , Óleos Vegetais/administração & dosagem , Cicatrização/efeitos dos fármacos , Administração Tópica , Animais , Cloranfenicol/administração & dosagem , Desoxirribonuclease I/administração & dosagem , Modelos Animais de Doenças , Fibrinolisina/administração & dosagem , Masculino , Ratos , Ratos Wistar
13.
Prev Vet Med ; 172: 104787, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31689581

RESUMO

The growth rate of female calves on dairy farms is one of the crucial factors which influence age at first calving, affecting lifetime lactation productivity of a dairy cow. Diets with adequate crude protein are necessary to support calf growth. The study objective was to determine the effects of nutritional advice and diet supplementation with Calliandra calothyrsus and Sesbania sesban on average daily weight gain in dairy calves on semi-commercial smallholder dairy farms in Kenya. This trial involved 155 calves from 73 smallholder dairy farms, in Kenya, randomly selected and allocated to either intervention or control groups. The intervention group received nutritional advice and seedlings of Calliandra calothyrsus and Sesbania sesban, while the control group did not receive these interventions. Every 1-2 months for 16 months, data on farm nutritional practices and management were collected in a questionnaire, and physical examinations were done to monitor weight and health status. Descriptive and univariable statistical analyses were conducted, and multivariable mixed linear regression models were used for identification of factors associated (P < 0.05) with the natural log transformation of weight gain of calves on a given farm, controlling for clustering of visits within calves. Feeding at least 0.2 kg/day (wet weight) of Calliandra or Sesbania to calves <6 months old resulted in 33.2% increase in daily weight gain, while controlling for confounding by breed and sex of the calf. For calves ≥6 months, there was a significant interaction between amount of hay fed and if calves were also fed on Calliandra or Sesbania. When no Calliandra or Sesbania supplementation was provided, the mean weight gain was low and relatively constant even with increasing amounts of hay. When Calliandra or Sesbania supplement was added to the diet, the mean weight gain increased from 0.17 kg to 0.48 kg when hay was fed at 1 and 5 kg, respectively, while controlling for confounding by amount of maize silage fed and the prevailing season. In conclusion, supplementation of calf diets with at least 0.2 kg/calf/day of Calliandra or Sesbania is beneficial for faster weight gains.


Assuntos
Ração Animal/análise , Bovinos/crescimento & desenvolvimento , Fabaceae/química , Sesbania/química , Ganho de Peso/efeitos dos fármacos , Animais , Dieta/veterinária , Suplementos Nutricionais/análise , Fazendas , Quênia , Distribuição Aleatória
14.
Isotopes Environ Health Stud ; 55(6): 526-531, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31597451

RESUMO

Drying protocols might alter the isotopic signatures of plant samples given that high temperatures can volatilize various organic compounds or delay the halting of physiological processes at lower drying temperatures. We thus evaluated the effect of four drying protocols on the carbon and nitrogen isotopic signatures for 23 species of plants. In particular, leaves were either freeze-dried, placed in a herbarium drying stove (ca. 50°C), in a gravity convection oven (80°C), or microwaved (900 W) in 2-minute pulses, until constant weight. For each species, neither treatment led to significantly different δ13C values, which ranged from -31.7 to -12.4‰. The δ15N values of 21 of the species considered were not affected by the drying protocols, ranging from -11.6 to -8.8‰. For Tillandsia makoyana, significant differences were observed between the freeze dried and the microwaved samples and between the freeze dried samples and those dried at 50°C for Macroptilium gibbosifolium.


Assuntos
Isótopos de Carbono/análise , Dessecação/métodos , Monitoramento Ambiental/métodos , Liofilização , Isótopos de Nitrogênio/análise , Folhas de Planta/química , Fabaceae/química , Temperatura Alta , Inquéritos e Questionários , Volatilização
15.
Arq Bras Cir Dig ; 32(3): e1451, 2019.
Artigo em Inglês, Português | MEDLINE | ID: mdl-31644671

RESUMO

BACKGROUND: Hypovolemic shock is a common disease in polytrauma patients and may develop ischemia in various organs, increasing morbidity and mortality. The bowel is usually most affected by this condition. AIM: To evaluate the effects of copaiba oil on the intestinal mucosa's injury of rats submitted to hypovolemic shock. METHOD: Fifteen rats were divided into three groups: sham - simulated surgery; ischemia - animals submitted to hypovolemic shock; and copaiba - animals submitted to hypovolemic shock previously treated with copaiba oil. Mean blood pressure, arterial blood gas after shock induction, degree of intestinal lesion and villus length were evaluated. RESULTS: The sham presented the lowest values of lactate and PaCO2 and the highest values of mean arterial pressure, pH and bicarbonate in relation to the other groups. The degree of mesenteric lesion was zero in the sham group; 3.00±1.00 in the ischemia group; and 3.00±0.71 in the copaiba group. The villus length was 173.60±8.42 in the sham, 142.77±8.33 in the ischemia and 143.01±9.57 in the copaiba group. There was a significant difference between the sham and the other groups (p<0.05); however, there not significant difference between groups Ischemia and copaiba. CONCLUSION: Administration of copaiba oil did not reduce the intestinal mucosa lesion of rats after hypovolemic shock.


Assuntos
Anti-Inflamatórios/farmacologia , Fabaceae/química , Mucosa Intestinal/efeitos dos fármacos , Óleos Vegetais/farmacologia , Choque , Animais , Anti-Inflamatórios/química , Anti-Inflamatórios/uso terapêutico , Modelos Animais de Doenças , Íleo/patologia , Mucosa Intestinal/patologia , Isquemia/tratamento farmacológico , Masculino , Óleos Vegetais/química , Óleos Vegetais/uso terapêutico , Distribuição Aleatória , Ratos Wistar , Choque/tratamento farmacológico
16.
Comput Biol Chem ; 83: 107137, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31639647

RESUMO

Xanthine oxidase (XO) is a form of xanthine oxidoreductase, a type of enzyme that plays a key role in the induction of hyperuricemia and raising superoxide radical level in blood. The present study was performed to evaluate the inhibitory activity of isoflavonoids (1-3) isolated from the ethyl acetate fraction of Apios americana, on the catalytic reaction mediated by XO. The isoflavonoids exhibited potential inhibitory activity within microgram/mL along with quenching effect towards XO. A reduction in the respective IC50 values was observed in the presence of high concentration of substrate. Molecular docking study revealed Ser876 and Arg880 as the key amino acids involved in the interaction of the enzyme with inhibitor. Apparently, the results demonstrated isoflavonoids as one of the natural products with a potential inhibitory effect on XO.


Assuntos
Produtos Biológicos/farmacologia , Inibidores Enzimáticos/farmacologia , Fabaceae/química , Isoflavonas/farmacologia , Xantina Oxidase/antagonistas & inibidores , Produtos Biológicos/química , Produtos Biológicos/isolamento & purificação , Inibidores Enzimáticos/química , Inibidores Enzimáticos/isolamento & purificação , Isoflavonas/química , Isoflavonas/isolamento & purificação , Simulação de Acoplamento Molecular , Estrutura Molecular , Relação Estrutura-Atividade , Xantina Oxidase/metabolismo
17.
Molecules ; 24(18)2019 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-31487934

RESUMO

The rattans of Spatholobus suberectus Dunn are a traditional Chinese medicine activating blood circulation and removing stasis. They have often been used for the traditional Chinese medicinal treatment of breast cancer in modern China. In this study, four novel isoflavanes (1-3 and 5) and four known analogues (4 and 6-8) were isolated from an ethanolic extract of the rattans of S. suberectus. Their structures were elucidated by extensive spectroscopic analyses and electronic circular dichroism studies. MCF-7 and MDA-MB-231 human breast cancer cell lines were used to evaluate the cytotoxic effects of the isolates. Interestingly, compounds 1 and 2 only inhibited the proliferation of MCF-7 cells, while compound 6 showed a selective cytotoxicity against MDA-MB-231 cells. However, compound 4 had significant cytotoxicity against both MCF-7 and MDA-MB-231 cell lines.


Assuntos
Antineoplásicos Fitogênicos/farmacologia , Fabaceae/química , Isoflavonas/farmacologia , Extratos Vegetais/farmacologia , Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/isolamento & purificação , Neoplasias da Mama , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Relação Dose-Resposta a Droga , Feminino , Humanos , Isoflavonas/química , Isoflavonas/isolamento & purificação , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Relação Estrutura-Atividade
18.
Mater Sci Eng C Mater Biol Appl ; 104: 109978, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31500000

RESUMO

Eysenhardtia polystachya (EP) is an endemic Mexican plant that has been widely studied for its antidiabetic, antibacterial, and antioxidant properties. Several studies had reported the main components of EP, but their fluorescence properties had not been broadly studied. In a previous study we obtained extracts with different composition from this plant and they presented florescence. In this work we study fluorescent compounds from EP and evaluate their fluorescence properties. EP extracts were obtained by Soxhlet extraction with ethanol, samples were dried, and compounds were separated by column chromatography. Fluorescent fractions were classified apart from other fractions and characterized by Scanning electron microscopy (SEM), UV-Vis, Raman, FTIR and 1H NMR spectra. Additionally, we obtained functional nanomaterials (using silica nanoparticles). TD-DFT molecular calculations of the fluorescent components were carried out to compare their theoretical UV-Vis spectra to experimental results. Nine fractions were obtained by chromatography and five of them showed fluorescence. Fluorescence of extracts from Eysenhardtia polystachya is due to more than one component and we suggest that could be other hydrochalcones for which we present possible structures. This finding would help to dissipate questions about which component is responsible for fluorescence in extracts from the plant and in this way determinate the appropriate use for these fluorophores. Finally, the application and viability as a biosensor using pulmonary epithelium fibroblast cell culture IMR-90 was proved, and in the concentration used are non-toxic materials.


Assuntos
Fabaceae/química , Corantes Fluorescentes/química , Extratos Vegetais/química , Técnicas Biossensoriais/métodos , Linhagem Celular , Epitélio/efeitos dos fármacos , Fibroblastos/efeitos dos fármacos , Fluorescência , Humanos , Pulmão/efeitos dos fármacos , Nanopartículas/química , Dióxido de Silício/química
19.
J Pharm Pharmacol ; 71(10): 1532-1543, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31378977

RESUMO

OBJECTIVES: Bioactive molecules derived from natural products combine the ability to absorb UV light and act as antioxidants. We developed an oil-based sucupira (native species of the Brazilian cerrado) nanoemulsion (NE) using a high-energy emulsification method and assessed its effectiveness in vitro. METHODS: An easily scalable high-pressure homogenization method was used to prepare the formulation. NE droplets mean diameter, pH, stability, conductivity and morphology were analysed. Formulation bioactivity was assessed using HaCaT cells. KEY FINDINGS: The formulation presented suitable pH and size for topic administration and was stable for over 90 days upon storage at 4, 25 and 45°C. The NE showed protective effect against oxidative stress and reduced levels of UVA-induced pro-inflammatory cytokines IL-6 and IL-8. CONCLUSIONS: A novel, stable and easily prepared formulation was obtained for encapsulation of sucupira oil. The protective effect of the formulation by cytokine inhibition in the early stage of the inflammatory process was shown in vitro. Combined with the antioxidant effect by inhibition of reactive oxygen species, the use of sucupira oil NE for prevention and treatment of UVA-induced stress could contribute to decrease the effects of UV radiation on skin ageing.


Assuntos
Emulsões/farmacologia , Fabaceae/química , Nanopartículas/química , Estresse Oxidativo/efeitos dos fármacos , Óleos Vegetais/farmacologia , Substâncias Protetoras/farmacologia , Antioxidantes/química , Antioxidantes/farmacologia , Brasil , Linhagem Celular , Emulsões/química , Humanos , Óleos Vegetais/química , Substâncias Protetoras/química , Espécies Reativas de Oxigênio/metabolismo , Pele/efeitos dos fármacos , Raios Ultravioleta/efeitos adversos
20.
Food Funct ; 10(9): 5931-5938, 2019 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-31469378

RESUMO

Legume flours contain elevated levels of healthy functional components. The aim of the present paper was to evaluate the levels of polyphenols and flavonoids and total antioxidant capacity of pasta obtained using 100% legume flours (100% pea flour, 100% red lentil flour, and 60% grass pea flour and 40% chickpea flour). Moreover, the post-prandial glycaemic responses, the glycaemic index and glycaemic load were evaluated. The levels of the bioactive compounds (total polyphenols and flavonoids) and the antioxidant properties were higher in all products obtained with legume flours compared with durum wheat semolina (DWS) pasta. After cooking by boiling, a decrease in the total content of polyphenols of about 50%-60% was observed in all pasta samples. The comparison of the post-prandial increase of glucose after intake of pasta obtained with 100% legume flours demonstrated significant differences compared with DWS pasta. Glycaemic index (GI) and glycaemic load (GL) values of DWS pasta were 52.59 ± 8.41% and 26.14 ± 3.12, respectively. The GI values and GL values were 20.07 ± 7.67% and 6.88 ± 2.63 in the product containing grass pea and chickpea flour, 22.38 ± 6.92% and 8.30 ± 2.56 in the product containing red lentil flour, and 23.39 ± 6.72% and 8.65 ± 2.50 in the product containing pea flour. The results suggest that pasta formulated with legume flours is a low glycaemic food and can be considered a suitable source of polyphenols and flavonoids in human diet.


Assuntos
Glicemia/metabolismo , Fabaceae/metabolismo , Polifenóis/metabolismo , Antioxidantes/química , Antioxidantes/metabolismo , Glicemia/análise , Culinária , Digestão , Fabaceae/química , Farinha/análise , Índice Glicêmico , Carga Glicêmica , Humanos , Valor Nutritivo , Polifenóis/química , Paladar
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