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2.
PLoS One ; 15(8): e0237491, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32841256

RESUMO

PURPOSE: Viral upper respiratory infections are associated with significant health and economic impact. This study sought to determine the efficacy of routine immune system micronutrient supplementation on the incidence, duration and severity of common cold symptoms. METHODS: This pilot study was a randomized, double-blinded, placebo-controlled trial of N = 259 with asymptomatic participants aged 18 to 65 in two cold seasons of 2016 and 2017. The treatment group received an immune system targeted micronutrient caplet, while the placebo group received a micronized cellulose caplet externally identical to the treatment caplet. Weekly surveys were sent electronically to participants to document common cold incidence, duration and severity. Primary statistical results were obtained using mixed-effects logistic regressions to account for longitudinal measurements for participants. RESULTS: The odds of acquiring an upper respiratory infection, adjusted for potential confounders, was estimated to be 0.74 times lower in the treatment group (p = 0.14). The odds of reporting specific symptoms were statistically lower in the treatment arm compared to the placebo arm for runny nose (OR = 0.53, p = 0.01) and cough (OR = 0.51, p = 0.04). Shorter durations of runny nose and cough were also observed in the treatment arm compared to placebo (both p < 0.05). There was no significant difference in severity of symptoms in either group. The observed proportion of reported cold symptoms in the treatment group was lower compared to the placebo group between late January and February in two consecutive cold seasons. Given the physical, workplace and economic impact of upper respiratory infections, this low cost and low risk intervention should be further studied with more robust investigation and meticulous experimental design.


Assuntos
Resfriado Comum/tratamento farmacológico , Micronutrientes/uso terapêutico , Adolescente , Adulto , Idoso , Resfriado Comum/complicações , Resfriado Comum/epidemiologia , Resfriado Comum/patologia , Tosse/patologia , Suplementos Nutricionais , Método Duplo-Cego , Feminino , Humanos , Incidência , Masculino , Pessoa de Meia-Idade , Razão de Chances , Faringite/patologia , Projetos Piloto , Efeito Placebo , Índice de Gravidade de Doença , Adulto Jovem
3.
BMJ Case Rep ; 12(12)2019 Dec 09.
Artigo em Inglês | MEDLINE | ID: mdl-31822531

RESUMO

Superior vena cava (SVC) syndrome is a group of symptoms caused by complete or partial obstruction of the flow of blood through the SVC. The obstruction is, in most cases, caused by the formation of thrombus or infiltration of a tumour through the vessel wall. The result is venous congestion that creates a clinical situation relating to increase in the venous pressure in the upper part of the body. Symptoms commonly associated with vena cava syndrome include cough, dyspnoea, swelling of the neck, face and the upper extremities and dilation of the chest vein collaterals. In this paper, we examine the case of a 50-year-old man who presented to the emergency department with 'sore throat' which can be easily misdiagnosed as a case of uncomplicated acute pharyngitis. It was a real challenge to diagnose our patient as SVC syndrome caused by bronchogenic carcinoma.


Assuntos
Carcinoma Broncogênico/diagnóstico , Neoplasias Pulmonares/diagnóstico , Faringite/patologia , Síndrome da Veia Cava Superior/diagnóstico , Tórax/patologia , Veia Cava Superior/patologia , Carcinoma Broncogênico/complicações , Humanos , Perda de Seguimento , Neoplasias Pulmonares/complicações , Excisão de Linfonodo , Masculino , Pessoa de Meia-Idade , Faringite/etiologia , Radiografia Torácica , Síndrome da Veia Cava Superior/complicações
4.
BMC Infect Dis ; 19(1): 1034, 2019 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-31805893

RESUMO

BACKGROUND: The incidence of Taralomyces marneffei infection in HIV-infected individuals has been decreasing, whereas its rate is rising among non-HIV immunodeficient persons, particularly patients with anti-interferon-gamma autoantibodies. T. marneffei usually causes invasive and disseminated infections, including fungemia. T. marneffei oro-pharyngo-laryngitis is an unusual manifestation of talaromycosis. CASE PRESENTATION: A 52-year-old Thai woman had been diagnosed anti-IFNÉ£ autoantibodies for 4 years. She had a sore throat, odynophagia, and hoarseness for 3 weeks. She also had febrile symptoms and lost 5 kg in weight. Physical examination revealed marked swelling and hyperemia of both sides of the tonsils, the uvula and palatal arches including a swelling of the epiglottis, and arytenoid. The right tonsillar biopsy exhibited a few intracellular oval and elongated yeast-like organisms with some central transverse septum seen, which subsequently grew a few colonies of T. marneffei on fungal cultures. The patient received amphotericin B deoxycholate 45 mg/dayfor 1 weeks, followed by oral itraconazole 400 mg/day for several months. Her symptoms completely resolved without complication. CONCLUSION: In patients with anti-IFN-É£ autoantibodies, T. marneffei can rarely cause a local infection involving oropharynx and larynx. Fungal culture and pathological examination are warranted for diagnosis T. marneffei oro-pharyngo-laryngitis. This condition requires a long term antifungal therapy.


Assuntos
Antifúngicos/uso terapêutico , Laringite/tratamento farmacológico , Micoses/tratamento farmacológico , Faringite/tratamento farmacológico , Talaromyces/patogenicidade , Anfotericina B/uso terapêutico , Autoanticorpos/sangue , Ácido Desoxicólico/uso terapêutico , Combinação de Medicamentos , Feminino , Humanos , Interferon gama/imunologia , Itraconazol/uso terapêutico , Laringite/microbiologia , Laringite/patologia , Pessoa de Meia-Idade , Infecções por Mycobacterium não Tuberculosas/tratamento farmacológico , Mycobacterium abscessus/patogenicidade , Micoses/etiologia , Micoses/microbiologia , Faringite/microbiologia , Faringite/patologia , Tailândia
5.
Immunol Res ; 67(4-5): 304-309, 2019 10.
Artigo em Inglês | MEDLINE | ID: mdl-31745821

RESUMO

Macroscopic hematuria concomitant with acute pharyngitis is a characteristic feature of immunoglobulin A nephropathy (IgAN). Although the underlying mechanism of worsening hematuria has not been fully elucidated, activation of the innate immune system of nasopharynx-associated lymphoid tissue is thought to play an important role. The epipharynx is an immunologically activated site even under normal conditions, and enhanced activation of innate immunity is likely to occur in response to airborne infection. As latent but significant epipharyngitis presents in most IgAN patients, it is plausible that acute pharyngitis due to airway infection may contribute as a trigger of the epipharyngeal innate immune system, which is already upregulated in the chronically inflamed environment. The aim of this review was to discuss the mechanism of epipharynx-kidney axis involvement in glomerular vasculitis responsible for the worsening of hematuria in IgAN.


Assuntos
Imunidade Inata , Glomérulos Renais , Faringite , Faringe , Glomerulonefrite por IGA/etiologia , Glomerulonefrite por IGA/imunologia , Glomerulonefrite por IGA/patologia , Humanos , Glomérulos Renais/imunologia , Glomérulos Renais/patologia , Faringite/complicações , Faringite/imunologia , Faringite/patologia , Faringe/imunologia , Faringe/patologia
6.
J Immunol ; 203(11): 3054-3067, 2019 12 01.
Artigo em Inglês | MEDLINE | ID: mdl-31645417

RESUMO

The pharyngeal organ is located at the crossroad of the respiratory and digestive tracts in vertebrate, and it is continuously challenged by varying Ags during breathing and feeding. In mammals, the pharyngeal mucosa (PM) is a critical first line of defense. However, the evolutionary origins and ancient roles of immune defense and microbiota homeostasis of PM are still unknown. In this study, to our knowledge, we are the first to find that diffuse MALT is present in PM of rainbow trout, an early vertebrate. Importantly, following parasitic infection, we detect that strong parasite-specific mucosal IgT and dominant proliferation of IgT+ B cell immune responses occurs in trout PM, providing, to our knowledge, the first demonstration of local mucosal Ig responses against pathogens in pharyngeal organ of a nonmammal species. Moreover, we show that the trout PM microbiota is prevalently coated with secretory IgT and, to a much lesser degree, by IgM and IgD, suggesting the key role of mucosal Igs in the immune exclusion of teleost pharyngeal bacteria. Overall, to our knowledge, our findings provide the first evidence that pharyngeal mucosal immunity appear earlier than tetrapods.


Assuntos
Evolução Biológica , Homeostase/imunologia , Oncorhynchus mykiss/imunologia , Faringite/imunologia , Mucosa Respiratória/imunologia , Animais , Faringite/patologia , Mucosa Respiratória/patologia
10.
Laryngoscope ; 129(4): 877-882, 2019 04.
Artigo em Inglês | MEDLINE | ID: mdl-30194702

RESUMO

OBJECTIVE: Human papillomavirus (HPV) has been identified as a risk factor for oropharyngeal squamous cell carcinoma (OPSCC) and a cause of the recent dramatic rise in the incidence of this disease. HPV-positive OPSCC typically affects a younger population and has no validated screening test. This study aims to outline the common presenting signs of HPV-positive OPSCC. METHODS: We conducted a retrospective single-institution review on 370 patients who were treated at the Icahn School of Medicine at Mount Sinai, New York City, between April 2007 and November 2015 for OPSCC. We included patients with newly diagnosed OPSCC and sufficient history and physical data in the final analysis. Univariate analysis was used to compare HPV-positive and HPV-negative cohorts for demographics, tumor location, tumor staging, initial presentation and symptoms, and physical exam findings. RESULTS: Two hundred and seven patients met the inclusion criteria. The most common initial symptoms for OPSCC were neck mass (51.7%) and sore throat (13.0%). The HPV-positive cohort was more likely to present with a chief complaint of neck mass compared to the HPV-negative cohort (56.1% vs. 22.2%; P = 0.0015). A positive neck exam was associated with HPV-positive status (73.9% vs. 40.7%; P = 0.0012). CONCLUSION: HPV-positive OPSCC is an incipient epidemic, poised to surpass cervical cancer as the most common HPV-related cancer by 2020. Initial presenting signs may often be mistaken for benign processes. This study provides physicians with a better understanding of initial presentation of patients with HPV-positive OPSCC, leading to earlier diagnosis and improved outcomes. LEVEL OF EVIDENCE: 4 Laryngoscope, 129:877-882, 2019.


Assuntos
Carcinoma de Células Escamosas/patologia , Neoplasias Orofaríngeas/patologia , Papillomaviridae , Infecções por Papillomavirus/patologia , Faringite/patologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Carcinoma de Células Escamosas/virologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Pescoço/patologia , Pescoço/virologia , Estadiamento de Neoplasias , Neoplasias Orofaríngeas/virologia , Infecções por Papillomavirus/virologia , Faringite/virologia , Estudos Retrospectivos , Fatores de Risco
12.
J Clin Microbiol ; 56(5)2018 05.
Artigo em Inglês | MEDLINE | ID: mdl-29491014

RESUMO

In September 2016, 140 patients with primary symptoms of sore throat and fever were identified in a school dormitory in Osaka, Japan. Epidemiological and laboratory investigations determined that these symptomatic conditions were from a foodborne outbreak of group G streptococcus (GGS), with GGS being isolated from samples from patients, cooks, and foods. The strain of GGS was identified as Streptococcus dysgalactiae subsp. equisimilis of two emm types (stG652.0 and stC36.0). The causative food, a broccoli salad, was contaminated with the two types of S. dysgalactiae subsp. equisimilis, totaling 1.3 × 104 CFU/g. Pulsed-field gel electrophoresis (PFGE) of samples from patients, cooks, and foods produced similar band patterns among samples with the same emm type. This result suggested the possibility of exposure from the contaminated food. The average onset time was 44.9 h and the prevalence rate was 62%. This is the first report to identify the causative food of a foodborne outbreak by Streptococcus dysgalactiae subsp. equisimilis.


Assuntos
Surtos de Doenças , Microbiologia de Alimentos , Faringite/epidemiologia , Instituições Acadêmicas , Infecções Estreptocócicas/epidemiologia , Streptococcus/isolamento & purificação , Anticorpos Antibacterianos/sangue , Antígenos de Bactérias/genética , Proteínas da Membrana Bacteriana Externa/genética , Brassica/microbiologia , Proteínas de Transporte/genética , DNA Bacteriano/genética , Eletroforese em Gel de Campo Pulsado , Humanos , Japão/epidemiologia , Faringite/diagnóstico , Faringite/patologia , Instituições Residenciais , Infecções Estreptocócicas/diagnóstico , Infecções Estreptocócicas/patologia , Streptococcus/genética , Streptococcus/crescimento & desenvolvimento , Streptococcus/imunologia
13.
Clin Rheumatol ; 37(5): 1309-1317, 2018 May.
Artigo em Inglês | MEDLINE | ID: mdl-28748511

RESUMO

The objective of this study is to compare the histology and immune cell composition of tonsils from patients with periodic fever, aphthous stomatitis, pharyngitis, and cervical adenitis (PFAPA) syndrome to those from patients with obstructive sleep apnea (OSA). Patients with PFAPA and age-matched controls with OSA who had undergone tonsillectomy at Vanderbilt Children's Hospital were recruited. After informed consent, archival paraffin-embedded, formalin-fixed tonsil tissues were obtained. Sizes of major histologic regions were measured. Cores of germinal centers, crypts, and squamous epithelium were assembled on a tissue microarray for immunohistochemical staining and digital image analysis. Features of tonsils from PFAPA and OSA patients were compared with Wilcoxon signed-rank test. Samples from 16 cases with PFAPA and 16 controls with OSA were evaluated. Tonsils from PFAPA cases had significantly smaller germinal centers (0.18 vs. 0.47 mm2, p = 0.001) and wider squamous epithelia (176 vs. 138 µm, p = 0.008) than those of OSA patients. The percentages of B and T lymphocytes and myeloid cells were comparable in germinal centers, crypts, and squamous epithelia from PFAPA and OSA patients. Longer time from the last febrile episode in PFAPA cases was associated with larger germinal center area (Spearman's rho = 0.61, p = 0.02). We found differences in the sizes of germinal centers and squamous epithelia in tonsils of patients with PFAPA and OSA, but the cellular compositions within these areas were comparable. Our results suggest that tonsils from patients with PFAPA change histologically over time with enlarging germinal centers following a febrile episode. Additional studies are needed to understand the pathogenesis of PFAPA.


Assuntos
Febre/patologia , Linfadenite/patologia , Tonsila Palatina/patologia , Faringite/patologia , Estomatite Aftosa/patologia , Criança , Pré-Escolar , Feminino , Humanos , Lactente , Masculino , Síndrome
14.
Pediatr Dev Pathol ; 21(5): 497-501, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-28882091

RESUMO

Periodic fever, aphthous stomatitis, pharyngitis, and cervical adenitis (PFAPA) syndrome is a common cause of periodic fever in children. The pathogenesis of PFAPA is unknown but likely involves immune system dysregulation and may be initiated by an environmental trigger. Tonsillectomy resolves or improves symptoms in some patients, but the reason for this is unknown; moreover, specific abnormalities in tonsillectomy specimens from PFAPA patients have not been described. Here, we report measles virus in tonsil from a child with PFAPA. Measles-type viral cytopathic effect was discovered on histological examination of tonsillar tissue after therapeutic tonsillectomy for PFAPA. Molecular testing showed the left tonsil was positive for measles RNA by reverse transcription polymerase chain reaction (RT-PCR) while the right tonsil was inconclusive (weakly positive). Real-time RT-PCR specific for measles vaccine strain RNA (genotype A) was weakly reactive in the left tonsil tissue when tested in 3 independent replicates, but this result could not be confirmed with conventional genotyping by sequencing. The relationship and clinical significance between measles virus and PFAPA in this case is unclear but may be related to PFAPA-associated immune dysregulation. Additional investigation of measles virus in PFAPA patients would be helpful in further exploring this potential association.


Assuntos
Febre/complicações , Linfadenite/complicações , Sarampo/complicações , Tonsila Palatina/patologia , Faringite/complicações , Estomatite Aftosa/complicações , Pré-Escolar , Febre/patologia , Humanos , Linfadenite/patologia , Masculino , Sarampo/patologia , Morbillivirus/isolamento & purificação , Faringite/patologia , Estomatite Aftosa/patologia , Síndrome
15.
BMJ Case Rep ; 20172017 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-29269361

RESUMO

Uvular necrosis is an extremely rare complication of gastroscopy. We describe the fifth published case of uvular necrosis following an uncomplicated diagnostic gastroscopy in a young man. Presentation with severe sore throat and inability to swallow saliva occurred within 24 hours of gastroscopy and resolved with conservative treatment.


Assuntos
Gastroscopia/efeitos adversos , Doença Iatrogênica , Necrose/patologia , Faringite/patologia , Úvula/patologia , Analgesia , Anestésicos Locais/uso terapêutico , Antibacterianos/uso terapêutico , Gastroscopia/instrumentação , Humanos , Lidocaína/uso terapêutico , Masculino , Necrose/etiologia , Faringite/etiologia , Doenças Raras , Resultado do Tratamento , Úvula/irrigação sanguínea , Úvula/lesões , Adulto Jovem
16.
Medwave ; 17(9): e7111, 2017 Dec 26.
Artigo em Espanhol, Inglês | MEDLINE | ID: mdl-29286352

RESUMO

INTRODUCTION: Pain associated to acute pharyngitis is a frequent cause of consultation. Usual care includes non-steroidal anti-inflammatories and antibiotics in selected cases, but pain relief is not always quick enough. The use of corticosteroids has been proposed as a therapeutic alternative, but its actual efficacy is matter of debate. METHODS: To answer this question we used Epistemonikos, the largest database of systematic reviews in health, which is maintained by screening multiple information sources, including MEDLINE, EMBASE, Cochrane, among others. We extracted data from the systematic reviews, reanalyzed data of primary studies, conducted a meta-analysis and generated a summary of findings table using the GRADE approach. RESULTS AND CONCLUSIONS: We identified eight systematic reviews including 11 studies overall, of which 10 were randomized trials. We concluded a short course of systemic corticosteroids reduces pain related to acute pharyngitis, without increasing the risk of adverse effects.


Assuntos
Glucocorticoides/uso terapêutico , Dor/tratamento farmacológico , Faringite/tratamento farmacológico , Doença Aguda , Antibacterianos/uso terapêutico , Anti-Inflamatórios/uso terapêutico , Bases de Dados Factuais , Glucocorticoides/administração & dosagem , Glucocorticoides/efeitos adversos , Humanos , Dor/etiologia , Faringite/patologia , Ensaios Clínicos Controlados Aleatórios como Assunto , Resultado do Tratamento
17.
Microb Pathog ; 113: 365-371, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-29146495

RESUMO

Chronic pharyngitis, a common inflammation of the pharyngeal mucosa, is often caused by bacteria, viruses, alcohol abuse, overuse of the voice and cigarettes. This study aimed to explore the effects of polysaccharides of Citrus grandis L. Osbeck (PCG) in relieving chronic pharyngitis and illustrate the underlying mechanisms. Polysaccharides were obtained from PCG by column chromatographic extraction. Six clinical symptom scores, such as the severity of itchy throat, hoarseness, pain, odynophagia, cough and otalgia were evaluated in chronic pharyngitis patients after the oral intake of PCG. The effects of polysaccharides on chronic pharyngitis were investigated in ammonia-stimulated rabbits through pathology analysis. The levels of inflammatory markers in the peripheral blood T cells were analyzed by ELISA. The total and phosphorylated levels of ERK1/2, JNK and p38 were assessed by Western blot. Protein amount of IKKα and p65, IKKα/ß activity and p65 activity were evaluated by Western blot and luciferase assay. The clinical studies presented that PCG significantly relieved the six symptoms of chronic pharyngitis patients. Pathology analysis of chronic pharyngitis animals showed that the PCG treatment groups showed a significant decrease in the number of pathologic cells and the reduction of pathologic cells was dose-dependent (p < 0.01). ELISA analysis showed that PCG significantly inhibited the αCD3-induced increase of IFN-γ, IL-2 and IL-4 expression in a dose-dependent manner. Moreover, Western blot and luciferase assay suggested that the phosphorylation of IKKα/ß in peripheral blood T cells was inhibited by the administration of PCG. These results indicate that polysaccharides exhibit anti-inflammatory effects by inhibiting the phosphorylation of IKKs, subsequently suppressing the NF-κB pathway activation and decreasing the expression of inflammatory mediators.


Assuntos
Citrus/química , Inflamação/tratamento farmacológico , Faringite/tratamento farmacológico , Extratos Vegetais/farmacologia , Polissacarídeos/farmacologia , Amônia/efeitos adversos , Animais , Citocinas/metabolismo , Modelos Animais de Doenças , Células HEK293 , Humanos , Quinase I-kappa B/metabolismo , Interferon gama/metabolismo , Interleucina-2/metabolismo , Interleucina-4/metabolismo , Sistema de Sinalização das MAP Quinases , Proteínas Quinases Ativadas por Mitógeno/metabolismo , Faringite/patologia , Faringite/fisiopatologia , Fosforilação , Coelhos , Linfócitos T/efeitos dos fármacos , Proteínas Quinases p38 Ativadas por Mitógeno
18.
Indian J Med Res ; 145(6): 758-766, 2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-29067978

RESUMO

BACKGROUND & OBJECTIVES: Rheumatic fever (RF) and rheumatic heart disease (RHD) are the autoimmune sequelae caused by Group A Streptococcus. RHD still remains a major concern in the developing countries due to its poor diagnosis, lack of vaccines and social awareness among population. This study was aimed to identify the plausible early- and late-stage disease markers associated with RF/RHD. METHODS: A total of 84 patients with confirmed pharyngitis (n=18), RF (n=23) and RHD (n=43) were included in the comparative analysis of different factors involved in host-pathogen interaction during RF/RHD pathogenesis. RESULTS: This study revealed high titre of serum antistreptolysin O (ASO) antibody in pharyngitis compared to RF and RHD patients, whereas procollagen type 1 C-peptide (PICP) level was elevated in RHD which showed an inverse correlation with serum ASO titre. The significant elevation of serum anti-peptide associated with RF (PARF) antibody in RF patients was correlated as a probable stage-specific determinant. In addition, pro-inflammatory cytokine profile revealed high levels of interleukin-12 (IL-12)/IL-23p40, IL-17A in RF, whereas IL-6 concentration was higher in RHD compared to healthy controls. INTERPRETATION & CONCLUSIONS: The overall assessment of the factors/ disease markers involved in host-pathogen interaction in RF/RHD may be suggestive of plausible disease marker in different groups of patients. Further studies with larger sample need to be done to better understand RF/RHD pathogenesis.


Assuntos
Biomarcadores/sangue , Faringite/sangue , Febre Reumática/sangue , Cardiopatia Reumática/sangue , Adolescente , Adulto , Idoso , Anticorpos/sangue , Antiestreptolisina/sangue , Criança , Pré-Escolar , Citocinas/sangue , Feminino , Interações Hospedeiro-Patógeno/genética , Humanos , Índia , Masculino , Lectina de Ligação a Manose/sangue , Pessoa de Meia-Idade , Fragmentos de Peptídeos/sangue , Faringite/genética , Faringite/microbiologia , Faringite/patologia , Pró-Colágeno/sangue , Febre Reumática/genética , Febre Reumática/microbiologia , Febre Reumática/patologia , Cardiopatia Reumática/genética , Cardiopatia Reumática/microbiologia , Cardiopatia Reumática/patologia , Streptococcus pyogenes/patogenicidade
19.
Infect Immun ; 85(11)2017 11.
Artigo em Inglês | MEDLINE | ID: mdl-28808160

RESUMO

Group A streptococci (GAS) are highly prevalent human pathogens whose primary ecological niche is the superficial epithelial layers of the throat and/or skin. Many GAS strains with a strong tendency to cause pharyngitis are distinct from strains that tend to cause impetigo; thus, genetic differences between them may confer host tissue-specific virulence. In this study, the FbaA surface protein gene was found to be present in most skin specialist strains but largely absent from a genetically related subset of pharyngitis isolates. In an ΔfbaA mutant constructed in the impetigo strain Alab49, loss of FbaA resulted in a slight but significant decrease in GAS fitness in a humanized mouse model of impetigo; the ΔfbaA mutant also exhibited decreased survival in whole human blood due to phagocytosis. In assays with highly sensitive outcome measures, Alab49ΔfbaA was compared to other isogenic mutants lacking virulence genes known to be disproportionately associated with classical skin strains. FbaA and PAM (i.e., the M53 protein) had additive effects in promoting GAS survival in whole blood. The pilus adhesin tip protein Cpa promoted Alab49 survival in whole blood and appears to fully account for the antiphagocytic effect attributable to pili. The finding that numerous skin strain-associated virulence factors make slight but significant contributions to virulence underscores the incremental contributions to fitness of individual surface protein genes and the multifactorial nature of GAS-host interactions.


Assuntos
Proteínas de Bactérias/genética , Proteínas de Transporte/genética , Regulação Bacteriana da Expressão Gênica , Infecções Estreptocócicas/microbiologia , Streptococcus pyogenes/genética , Streptococcus pyogenes/patogenicidade , Animais , Proteínas de Bactérias/metabolismo , Células Sanguíneas/imunologia , Células Sanguíneas/microbiologia , Proteínas de Transporte/metabolismo , Modelos Animais de Doenças , Frutose-Bifosfato Aldolase , Aptidão Genética , Interações Hospedeiro-Patógeno , Humanos , Impetigo/imunologia , Impetigo/microbiologia , Impetigo/patologia , Camundongos , Faringite/imunologia , Faringite/microbiologia , Faringite/patologia , Faringe/imunologia , Faringe/microbiologia , Faringe/patologia , Isoformas de Proteínas/genética , Isoformas de Proteínas/metabolismo , Pele/imunologia , Pele/microbiologia , Pele/patologia , Infecções Estreptocócicas/imunologia , Infecções Estreptocócicas/patologia , Streptococcus pyogenes/metabolismo , Virulência
20.
Avian Dis ; 61(2): 255-260, 2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28665721

RESUMO

Infectious laryngotracheitis (ILT) is a contagious viral respiratory disease of great economic importance for the global poultry industry caused by Gallid herpesvirus 1 (GaHV-1). Lesions of the upper digestive tract caused by this virus have not been reported before. Two small flocks of backyard chickens experienced an outbreak of ILT, one in 2006 and the other in 2014. These birds had typical ILT lesions, characterized by a necrohemorrhagic laryngitis and tracheitis but were also affected by a severe erosive and necrotic esophagitis and pharyngitis. On microscopic examination of the esophagus and pharynx, numerous individual epithelial cells were degenerated or necrotic. Syncytial cells were present in the mucosa or sloughed in the overlying inflammatory crust, and some of these cells contained an amphophilic intranuclear viral inclusion. GaHV-1 was detected in tissues, from respiratory and digestive tracts, either by PCR, immunohistochemistry, or both diagnostic assays. This case stresses the importance for veterinarians, owners, and technicians to pay attention to different or atypical clinical manifestations of ILT given its highly contagious nature.


Assuntos
Esofagite/veterinária , Infecções por Herpesviridae/veterinária , Herpesvirus Galináceo 1/isolamento & purificação , Faringite/veterinária , Doenças das Aves Domésticas/virologia , Traqueíte/veterinária , Animais , Galinhas , Esofagite/patologia , Esofagite/virologia , Infecções por Herpesviridae/patologia , Infecções por Herpesviridae/virologia , Herpesvirus Galináceo 1/classificação , Herpesvirus Galináceo 1/genética , Faringite/patologia , Faringite/virologia , Doenças das Aves Domésticas/patologia , Traqueíte/patologia , Traqueíte/virologia
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