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1.
Medicine (Baltimore) ; 98(48): e18189, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31770275

RESUMO

Sparse is the research on the relationship between iron metabolism and mild cognitive impairment (MCI) in adults aged over 60 years with non-alcoholic fatty liver disease (NAFLD). The soluble transferrin receptor (sTfR), serum iron (SI), serum ferritin (SF), transferrin (TRF) and hemoglobin (HB) are indicators of iron metabolism.This study examined whether iron metabolism is associated with cognitive impairment in older individuals.A cross-sectional study was held in patients from a Chinese center. Individuals with NAFLD aged over 60 years were included if they did not have excessive alcohol intake and were free of stroke or dementia. Their cognitive function was assessed by the same neurologist. 3.0T H proton magnetic resonance spectroscopy (H-MRS) was performed to evaluate the hippocampus of the participants without contraindication. t test and Chi-square test were used to analyze the data. Binary logistic regression was used for correlation analysis.Fifty four (54%) of participants were diagnosed with MCI by the psychiatrist. MCI was significantly associated with higher sTfR after adjustment of all the covariates (OR = 2.565, 95%CI: 1.334∼4.934; P = .005). No statistically significant associations were observed between MCI and age or blood glucose or choline (Cho) /creatine (Cr) of theright hippocampus head.Increased age and low levels of sTfR and HB were associated with MCI in NAFLD individuals aged over 60 years.


Assuntos
Disfunção Cognitiva , Hemoglobinas/análise , Hipocampo/diagnóstico por imagem , Ferro , Hepatopatia Gordurosa não Alcoólica , Receptores da Transferrina/sangue , Idoso , China/epidemiologia , Disfunção Cognitiva/diagnóstico , Disfunção Cognitiva/epidemiologia , Disfunção Cognitiva/metabolismo , Correlação de Dados , Estudos Transversais , Feminino , Ferritinas/análise , Humanos , Ferro/sangue , Ferro/metabolismo , Espectroscopia de Ressonância Magnética/métodos , Masculino , Pessoa de Meia-Idade , Hepatopatia Gordurosa não Alcoólica/epidemiologia , Hepatopatia Gordurosa não Alcoólica/metabolismo , Hepatopatia Gordurosa não Alcoólica/psicologia , Transferrina/análise
2.
Medicina (Kaunas) ; 55(6)2019 Jun 03.
Artigo em Inglês | MEDLINE | ID: mdl-31163711

RESUMO

Background and objectives: Data suggests that nearly 30% of the general population have steatosis and up to 5% of this population develops nonalcoholic steatohepatitis (NASH). Liver biopsy is still considered to be the gold standard for the diagnosis of NASH. Great effort is being made toward the identification of sensitive diagnostic tests that do not involve invasive procedures to address a common concern in patients with the nonalcoholic fatty liver disease-whether they have NASH or simple steatosis. We aimed to investigate the independent predictors and develop a non-invasive, easy-to-perform, low-cost set of parameters that may be used in clinical practice to differentiate simple steatosis from NASH. Methods: А cross-sectional study of nonalcoholic fatty liver disease (NAFLD) patients divided into two groups: group I-simple steatosis (SS) and group II-biopsy-proven NASH. Strict inclusion criteria and stepwise analysis allowed the evaluation of a vast number of measured/estimated parameters. Results: One hundred and eleven patients were included-82 with simple steatosis and 29 with biopsy-proven NASH. The probability of NASH was the highest when homeostatic model assessment of insulin resistance (HOMA-IR) was above 2.5, uric acid above 380 µmol/L, ferritin above 100 µg/L and ALT above 45 U/L. An acronym of using first letters was created and named the HUFA index. This combined model resulted in an area under the receiver operator characteristic curve (AUROC) of 0.94, provided sensitivity, specificity, positive predictive value and a negative predictive value for NASH of 70.3%, 95.1%, 83.1% and 90.0%, respectively. Conclusion: We suggest a simple non-invasive predictive index HUFA that encompasses four easily available parameters (HOMA-IR, uric acid, ferritin and ALT) to identify patients with NASH, which may reduce the need for a liver biopsy on a routine basis in patients with NAFLD.


Assuntos
Fígado Gorduroso/sangue , Hepatopatia Gordurosa não Alcoólica/sangue , Adulto , Idoso , Análise de Variância , Biomarcadores/análise , Biomarcadores/sangue , Distribuição de Qui-Quadrado , Estudos Transversais , Fígado Gorduroso/diagnóstico , Fígado Gorduroso/fisiopatologia , Feminino , Ferritinas/análise , Ferritinas/sangue , Humanos , Resistência à Insulina/fisiologia , Masculino , Pessoa de Meia-Idade , Hepatopatia Gordurosa não Alcoólica/diagnóstico , Hepatopatia Gordurosa não Alcoólica/fisiopatologia , Curva ROC , Índice de Gravidade de Doença , Estatísticas não Paramétricas , Ácido Úrico/análise , Ácido Úrico/sangue
3.
Klin Lab Diagn ; 64(1): 24-28, 2019.
Artigo em Russo | MEDLINE | ID: mdl-30912880

RESUMO

The review presents the pathobiochemical and molecular mechanisms of sputum formation in patients with cystic fibrosis associated with the pathophysiological features of the disease. Statistical data on the prevalence of this pathology in the world and in the Russian Federation are presented. The mechanisms of sputum formation and disorders of the mucociliary apparatus, leading to the accumulation of viscous bronchopulmonary secret in cystic fibrosis, are considered. The principles of the relationship between the rheological properties of sputum and the formation of inflammation in the lungs with the addition of a concomitant specific microflora in the bronchopulmonary system in patients with cystic fibrosis are presented. Describes the opportunities for biochemical studies of sputum of patients with this pathology: determining the activity of enzymes (myeloperoxidase), the content of proteinase inhibitors (α2-macroglobulin and α1-antitrypsin) and proinflammatory cytokines (IL-8 and TNFa), concentrations of iron and ferriferous proteins (lactoferrin and ferritin), which makes biochemical studies of sputum available, non-invasive, quick and cost-effective method of diagnosis, which can be widely used as an auxiliary laboratory method and makes it possible to use these metabolites as diagnostic markers to assess the severity of inflammation and infection of the lower respiratory tract and predict the development of respiratory complications in patients with cystic fibrosis.


Assuntos
Fibrose Cística/diagnóstico , Escarro/química , Citocinas/análise , Ferritinas/análise , Humanos , Inflamação/fisiopatologia , Lactoferrina/análise , Peroxidase/análise , Inibidores de Proteases/análise
4.
Intern Emerg Med ; 14(3): 365-370, 2019 04.
Artigo em Inglês | MEDLINE | ID: mdl-29948832

RESUMO

Heart disease remains a leading cause of morbidity and mortality in transfusion-dependent thalassemia (TDT), which can be attributed to several factors but primarily develops in the setting of iron overload. This was a retrospective cohort study utilizing Webthal® patient data from five major centers across Italy. Patients without heart disease were followed-up for 10 years (2000-2010) and data were collected for demographics, splenectomy status, serum ferritin and hemoglobin levels, and comorbidities associated with heart disease. Among 379 patients analyzed (mean age 22.9 ± 5.1 years, 47.8% men), 44 (cumulative incidence: 11.6%) developed heart disease during the period of observation. Splenectomy (p = 0.002) and serum ferritin level (p < 0.001) were the only risk factors with significant association with heart disease. A serum ferritin threshold of ≥ 3000 ng/mL was the best predictor for the development of heart disease (86.4% sensitivity and 92.8% specificity, AUC: 0.912, 95% CI 0.852-0.971, p < 0.001). On multivariate analysis, only a serum ferritin level ≥ 3000 ng/mL remained significantly and independently associated with increased risk of heart disease (HR: 44.85, 95% CI 18.85-106.74), with a 5- and 10-year heart disease-free survival of 58 and 39%. The association between iron overload and heart disease in patients with TDT is confirmed, yet a new serum ferritin level of 3000 ng/mL to flag increased risk is suggested.


Assuntos
Ferritinas/análise , Cardiopatias/complicações , Talassemia/complicações , Talassemia/terapia , Adolescente , Adulto , Área Sob a Curva , Transfusão de Sangue/métodos , Transfusão de Sangue/tendências , Distribuição de Qui-Quadrado , Criança , Estudos de Coortes , Feminino , Ferritinas/efeitos adversos , Ferritinas/sangue , Cardiopatias/fisiopatologia , Humanos , Itália , Masculino , Curva ROC , Estudos Retrospectivos , Fatores de Risco , Análise de Sobrevida , Talassemia/fisiopatologia
5.
Nutrients ; 10(12)2018 Dec 08.
Artigo em Inglês | MEDLINE | ID: mdl-30544799

RESUMO

Several human interventions have indicated that Lactobacillus plantarum 299v (L. plantarum 299v) increases intestinal iron absorption. The aim of the present study was to investigate possible effects of L. plantarum 299v on the mechanisms of iron absorption on the cellular level. We have previously shown that lactic fermentation of vegetables increased iron absorption in humans. It was revealed that the level of ferric iron [Fe (H2O)5]2+ was increased after fermentation. Therefore, we used voltammetry to measure the oxidation state of iron in simulated gastrointestinal digested oat and mango drinks and capsule meals containing L. plantarum 299v. We also exposed human intestinal co-cultures of enterocytes and goblet cells (Caco-2/HT29 MTX) to the supplements in order to study the effect on proteins possibly involved (MUC5AC, DCYTB, DMT1, and ferritin). We detected an increase in ferric iron in the digested meals and drinks containing L. plantarum 299v. In the intestinal cell model, we observed that the ferric reductase DCYTB increased in the presence of L. plantarum 299v, while the production of mucin (MUC5AC) decreased independently of L. plantarum 299v. In conclusion, the data suggest that the effect of L. plantarum 299v on iron metabolism is mediated through driving the Fe3+/DCYTB axis.


Assuntos
Grupo dos Citocromos b/metabolismo , Suplementos Nutricionais/microbiologia , Ferritinas/metabolismo , Ferro na Dieta/farmacologia , Lactobacillus plantarum , Oxirredutases/metabolismo , Células CACO-2 , Técnicas de Cocultura , Ferritinas/análise , Células HT29 , Humanos , Fatores de Transcrição/metabolismo , Regulação para Cima/efeitos dos fármacos
6.
Biomed Microdevices ; 20(4): 91, 2018 10 25.
Artigo em Inglês | MEDLINE | ID: mdl-30361769

RESUMO

A rapid, sensitive and quantitative biomarker detection platform is of great importance to the small clinic or point-of-care (POC) diagnosis. In this work, we realize that an automated diagnostic platform mainly includes two components: (1) an instrument that can complete all steps of the chemiluminescence immunoassay automatically and (2) an integrated microfluidic chip which is disposable and harmless. In the instrument, we adopt vacuum suction cups which are driven by linear motor to realize a simple, effective and convenient control. The method of acridine esterification chemiluminescence is adopted to achieve a quantitative detection, and a photomultiplier tube is used to detect photons from acridine ester producing in alkaline conditions. We use the laser cutting machine and hot press machine to accomplish the product of microfluidic chips. The automated microfluidics-based system is demonstrated by implementation of a chemiluminescence immunoassay for quantitative detection of ferritin. We observe alinear relationship between CL intensity and the concentration of ferritin from 5.1 to 1300 ng mL -1and the limit of detection (LoD) is 2.55 ng mL -1. At the same time, we also used the automated microfluidics-based system to test clinical serum samples. The whole process of chemiluminescence experiment can complete within 45 min. We realize that this lab-on-a-chip chemiluminescence immunoassay platform with features of automation and quantitation provides a promising strategy for POC diagnosis.


Assuntos
Biomarcadores/análise , Imunoensaio/instrumentação , Dispositivos Lab-On-A-Chip , Medições Luminescentes/instrumentação , Automação , Custos e Análise de Custo , Ferritinas/análise , Dispositivos Lab-On-A-Chip/economia
7.
Neth J Med ; 76(8): 365-373, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30362946

RESUMO

BACKGROUND: Type 2A hereditary haemochromatosis (type 2A HH) is a rare iron-loading disorder caused by mutations in the HFE2 gene, which encodes the HJV protein. We present characteristics, treatment and follow-up of subjects diagnosed with type 2A HH in the Netherlands to increase awareness of the disease and its treatment, and to define knowledge gaps. METHODS: We collected clinical, biochemical and genetic data from seven patients (two female; five probands) from six families genetically diagnosed with type 2A HH at the Expertise Center for Iron Disorders, Radboud University Medical Centre between 2006 and 2016. RESULTS: The five probands presented with heterogeneous complaints between the ages of 19 and 39. One of two patients with delayed clinical diagnosis developed hypogonadism and Y. enterocolitica sepsis. Diagnostic workup and follow-up varied. When assessed, elevated transferrin saturation (79-98%), ferritin (1400-6200 µg/l) and severely elevated liver iron levels were found, and in all subjects, phlebotomies were initiated. One subject was switched to erythrocytapheresis. Target ferritin levels varied. Despite long-term iron depletion, two subjects developed clinical complications. Sanger sequencing revealed two pathogenic HFE2 variants (homozygous or compound heterozygous) for the five families of Dutch descent and one new pathogenic variant in the family of non-Dutch descent. CONCLUSION: Three genetic variants caused type 2A HH in six families. Clinical diagnosis was delayed in two subjects. We observed variance in presentation, workup, follow-up and treatment. We found new complications in long-term iron-depleted patients. We recommend research and guidelines for optimal workup, follow-up and treatment of type 2A HH.


Assuntos
Predisposição Genética para Doença/genética , Hemocromatose/congênito , Adolescente , Adulto , Ferritinas/análise , Hemocromatose/diagnóstico , Hemocromatose/genética , Hemocromatose/terapia , Humanos , Ferro/análise , Fígado/metabolismo , Masculino , Mutação , Países Baixos , Linhagem , Estudos Retrospectivos , Adulto Jovem
8.
Resuscitation ; 130: 88-91, 2018 09.
Artigo em Inglês | MEDLINE | ID: mdl-29981817

RESUMO

OBJECTIVE: To evaluate the effect of cord milking on short term morbidity and hematologic parameters at 6 weeks in preterm neonates requiring resuscitation. METHODS: This trial randomized preterm infants requiring resuscitation to milking group and no milking group. Multiple pregnancy, Rh negative mothers, hydrops, cord abnormalities were excluded. The primary outcome was hemoglobin and serum ferritin at 6 weeks of life. Secondary outcomes were common preterm morbidities and mortality. RESULTS: 60 neonates were included in the study. Infants in the milking group had higher hemoglobin (10.07 g/dl vs 8.9 g/dl; p 0.003) and higher serum ferritin level (244.8 ng/ml vs 148.5 ng/ml; p 0.04) compared to no milking group. CONCLUSIONS: In preterm neonates requiring resuscitation, umbilical cord milking results in higher hemoglobin and ferritin at 6 weeks of life. It can be a used as a placental transfusion strategy in preterm neonates requiring resuscitation with no significant adverse effects. CLINICAL TRIAL REGISTRATION: Clinical trials registry -India CTRI/2015/01/005436, www.ctri.nic.in.


Assuntos
Transfusão de Sangue/métodos , Ferritinas/análise , Sangue Fetal , Hemoglobinas/análise , Recém-Nascido Prematuro/fisiologia , Circulação Placentária , Ressuscitação/métodos , Constrição , Feminino , Humanos , Recém-Nascido , Masculino , Gravidez , Fatores de Tempo , Resultado do Tratamento , Cordão Umbilical/fisiologia
9.
Am J Trop Med Hyg ; 99(2): 489-494, 2018 08.
Artigo em Inglês | MEDLINE | ID: mdl-29893201

RESUMO

Environmental enteric dysfunction (EED) causes gut inflammation and increased intestinal permeability leading to deficiencies in micronutrients such as zinc and iron. Fecal markers such as myeloperoxidase (MPO), neopterin (NEO), and alpha-1-anti-trypsin (AAT) can predict EED. The aim of this study was to examine the association between fecal markers of EED with zinc and iron status among children at first 2 years of life. Malnutrition and Enteric Disease Study Bangladeshi birth cohort data were used to conduct this analysis. Multivariable analyses using generalized estimating equations were performed to test the association between individual fecal markers with zinc or iron status of the children. A total of 265 children were enrolled in the study (male:female = 1:1). Of the 627 stool samples collected (N = 222 children), 535, 511, and 577 were accompanied by zinc, ferritin, and soluble transferrin receptor values, respectively. Median (interquartile range [IQR]) values of AAT, MPO, and NEO were 0.33 (0.18-0.62) mg/g, 3,895.42 (1,563.76-8,432.82) ng/mL, and 890.81 (331.57-2,089.04) nmol/L, respectively. Overall, 60%, 71%, and 97% of samples were above the values considered normal in nontropical settings for AAT, MPO, and NEO, respectively. High AAT levels were significantly associated with low ferritin values after adjusting for age and gender (coefficient = -5.85; 95% confidence interval = -11.23 to -0.47; P value = 0.03). No such association was found between AAT and plasma zinc status. Myeloperoxidase and NEO were not associated with plasma zinc or iron status. The study results imply the importance of enteric protein loss in contributing to reduced ferritin levels at first 2 years of life.


Assuntos
Fezes/química , Enteropatias/fisiopatologia , Ferro/sangue , Zinco/sangue , Bangladesh , Biomarcadores/análise , Feminino , Ferritinas/análise , Transtornos do Crescimento/sangue , Transtornos do Crescimento/diagnóstico , Humanos , Lactente , Recém-Nascido , Inflamação , Enteropatias/diagnóstico , Ferro/deficiência , Masculino , Peroxidase/análise , Áreas de Pobreza , Zinco/deficiência , alfa 1-Antitripsina/análise
11.
J Histochem Cytochem ; 66(11): 825-839, 2018 11.
Artigo em Inglês | MEDLINE | ID: mdl-29873589

RESUMO

Little is known about the renal responses to acute iron overloading. This study measured the renal tubular expression of transferrin receptor-1 (TfR1), cubilin/megalin receptors, hepcidin, ferroportin, and ferritin chains following subacute intoxication of 40 male Wistar rats with a single oral dose of ferrous iron (300 mg/kg). The animals were randomly subdivided into 4 equal subgroups at the time of necropsy (1, 2, 4, and 8 hr). The results were compared with the controls ( n=15) and with the chronic group ( n=15), which received iron for 4 weeks (75 mg/kg/day; 5 days/week). Although both toxicity models inhibited TfR1, they upregulated the cubilin/megalin receptors and hepcidin, and triggered iron deposition in tubular cells. The ferritin heavy-chain and ferroportin were downregulated in the 2-hr and 4-hr acute subgroups, whereas chronic toxicity promoted their expression, compared with controls. Moreover, the 4-hr and 8-hr subgroups had higher intracellular Fe+2 and marked cell apoptosis compared with the chronic group. In conclusion, the kidney appears to sustain iron reabsorption in both intoxication models. However, the cellular iron storage and exporter proteins were differentially expressed in both models, and their inhibition post-acute toxicity might contribute toward the intracellular accumulation of Fe+2, oxidative stress, and ferroptosis.


Assuntos
Sobrecarga de Ferro/patologia , Ferro/análise , Rim/patologia , Doença Aguda , Animais , Apoptose , Caspases/análise , Doença Crônica , Ensaio de Imunoadsorção Enzimática/métodos , Ferritinas/análise , Ferritinas/sangue , Imunofluorescência/métodos , Hepcidinas/análise , Hepcidinas/sangue , Ferro/sangue , Sobrecarga de Ferro/sangue , Rim/metabolismo , Proteína-2 Relacionada a Receptor de Lipoproteína de Baixa Densidade/análise , Masculino , Estresse Oxidativo , Ratos Wistar , Receptores de Superfície Celular/análise , Receptores da Transferrina/análise
12.
J Womens Health (Larchmt) ; 27(10): 1225-1232, 2018 10.
Artigo em Inglês | MEDLINE | ID: mdl-29957101

RESUMO

BACKGROUND: To investigate the efficacy and safety of estradiol valerate (EV)/dienogest (DNG) for the management of heavy menstrual bleeding (HMB) in Asian and non-Asian women desiring contraception. MATERIALS AND METHODS: In this multicenter, double-blind, phase III study, women were randomized 2:1 to receive EV/DNG or placebo tablets daily for seven 28-day cycles. The primary endpoint was the absolute change in menstrual blood loss (MBL) volume between the run-in and efficacy phases (90 days each). Secondary endpoints included the proportion of women with successful treatment (i.e., no episodes of MBL ≥80 mL and a decrease of <50% in MBL), percent change in MBL from the run-in phase, and change in hemoglobin and serum ferritin levels. Adverse events (AEs) were monitored throughout the study. RESULTS: Of the 341 women (mean age 34.7 ± 7.7 years; 309 Asians, 32 non-Asians) randomized, 270 completed the study. Mean reduction in MBL volume from run-in phase was significantly greater with EV/DNG than placebo (366.75 mL vs. 149.14 mL; p < 0.0001), with ∼52% and 12% of women, respectively, experiencing successful treatment. Percent decrease in MBL volume from the run-in phase was significantly greater with EV/DNG than placebo (63.5% vs. 24.8%; p < 0.0001). Hemoglobin and serum ferritin levels were increased with EV/DNG compared with placebo. Study drug-related AEs were reported in 16.3% and 8.2% of women with EV/DNG and placebo, respectively, none of which were of severe intensity. CONCLUSIONS: EV/DNG may be a safe and effective option in the treatment of HMB in Asian and non-Asian women who desire contraception.


Assuntos
Estradiol/análogos & derivados , Menorragia/tratamento farmacológico , Nandrolona/análogos & derivados , Adulto , Anticoncepcionais Orais/administração & dosagem , Anticoncepcionais Orais/efeitos adversos , Estradiol/administração & dosagem , Estradiol/efeitos adversos , Terapia de Reposição de Estrogênios/métodos , Estrogênios/administração & dosagem , Estrogênios/efeitos adversos , Feminino , Ferritinas/análise , Hemoglobinas/análise , Humanos , Menorragia/sangue , Ciclo Menstrual , Nandrolona/administração & dosagem , Nandrolona/efeitos adversos , Resultado do Tratamento
13.
BMC Oral Health ; 18(1): 63, 2018 04 10.
Artigo em Inglês | MEDLINE | ID: mdl-29636044

RESUMO

BACKGROUND: Iron disorder and abnormal expression of hepcidin play important roles in many diseases, but it is still unclear in chronic periodontitis (CP) and type 2 diabetes mellitus (T2DM). We aimed to assess ferritin and hepcidin levels in serum and saliva of CP patients with or without T2DM. METHODS: Serum and unstimulated whole saliva samples were collected from 88 participants, who were categorized into 4 groups based on the presence or absence of CP or T2DM. Demographics and general health parameters were recorded. Full-mouth clinical periodontal parameters including probing pocket depth, clinical attachment loss, bleeding index, and plaque index were recorded. Chemiluminescence microparticle immunoassay and enzyme-linked immunosorbent assay were used to detect ferritin and hepcidin concentrations, respectively, in serum and saliva. RESULTS: Serum ferritin and hepcidin levels in the CP and CP with T2DM groups were higher than in the control group (P < 0.05). Serum hepcidin and serum ferritin are linear correlated (P < 0.001). Serum hepcidin/ferritin values in the CP with T2DM group were significantly lower than those in the T2DM and control groups. Moreover, salivary ferritin levels in the CP and T2DM groups were higher than those in the control group (P < 0.05). There was positively correlation between salivary ferritin and serum ferritin (P = 0.017). Hepcidin concentrations were relatively low in saliva. CONCLUSIONS: These results suggest that iron overload and hepcidin inadequacy existed in CP with T2DM patients. Salivary ferritin might provide a reference for body iron load. TRIAL REGISTRATION: ChiCTR-ROC-17012780.


Assuntos
Periodontite Crônica/sangue , Diabetes Mellitus Tipo 2/sangue , Ferritinas/sangue , Hepcidinas/sangue , Saliva/química , Adulto , Idoso , Idoso de 80 Anos ou mais , Estudos de Casos e Controles , Periodontite Crônica/complicações , Índice de Placa Dentária , Diabetes Mellitus Tipo 2/complicações , Feminino , Ferritinas/análise , Hepcidinas/análise , Humanos , Masculino , Pessoa de Meia-Idade , Perda da Inserção Periodontal/sangue , Índice Periodontal
14.
Clín. investig. ginecol. obstet. (Ed. impr.) ; 45(1): 7-11, ene.-mar. 2018. tab, graf
Artigo em Espanhol | IBECS | ID: ibc-170098

RESUMO

Objetivo: Relacionar las concentraciones de ferritina plasmática materna en el tercer trimestre del embarazo con el crecimiento fetal. Materiales y métodos: Se seleccionaron pacientes con embarazos simples en el tercer trimestre que presentaron partos a término y que fueron atendidas en el Hospital Central «Dr. Urquinaona», Maracaibo, Venezuela. Se evaluaron la edad materna, edad gestacional al momento de la toma de la muestra y del parto, valores de hematocrito, concentraciones plasmáticas de ferritina materna y peso del recién nacido. Resultados: Se seleccionaron 131 pacientes embarazadas en el tercer trimestre a las cuales se les pudo realizar las mediciones de ferritina. La edad promedio de las pacientes fue de 30,6±4,1años. Las concentraciones promedio de ferritina fueron de 31,7±14,7ng/dl y el peso promedio de los recién nacidos fue de 3.194±434g. Al dividir las concentraciones de ferritina materna en cuartiles (concentraciones bajas, medias, altas y muy altas), se observó que las pacientes con concentraciones muy altas y altas de ferritina materna tenían recién nacidos con pesos significativamente más bajos que las pacientes con concentraciones bajas de ferritina (p<0,001). Se observó una correlación moderada, negativa y significativa entre la concentración de ferritina materna y el peso de los recién nacidos (r=-0,481; p<0,001). Conclusión: Existe una relación negativa y significativa de las concentraciones de ferritina plasmática materna en el tercer trimestre del embarazo con el crecimiento fetal


Objective: To relate maternal plasma ferritin concentrations during the third trimester of pregnancy to foetal growth. Materials and method: Patients with single pregnancies in the third trimester who had term deliveries and were cared for at the Hospital Central 'Dr. Urquinaona', Maracaibo, Venezuela were selected. Maternal age, gestational age at moment of sample and delivery, haematocrit values, maternal plasma ferritin concentrations and birth weight were evaluated. Results: We selected 131 patients in the third trimester of pregnancy in whom ferritin measurements could be performed. The mean age of the patients was 30.6±4.1 years. The mean ferritin concentration was 31.7±14.7ng/dl and the mean birth weight of the newborns was 3,194±434g. When the ferritin concentrations of the mothers were divided in quartiles (low, medium, high and very high concentrations), it was apparent that patients with very high and high concentrations of ferritin had newborns with significant lower birth weight than patients with low concentrations of ferritin (P<.001). A moderate, negative and significant correlation between maternal plasma ferritin concentration and newborn birth weight was also observed (r=−.481; P<.001). Conclusion: There is a negative and significant relationship between maternal plasma ferritin concentrations during the third trimester of pregnancy and foetal growth


Assuntos
Humanos , Feminino , Gravidez , Adulto , Ferritinas/análise , Terceiro Trimestre da Gravidez/fisiologia , Desenvolvimento Fetal/fisiologia , Idade Materna , Idade Gestacional , Estudos Longitudinais , Estudos Prospectivos , Análise de Variância
15.
Acta Orthop Traumatol Turc ; 52(2): 143-147, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29305046

RESUMO

OBJECTIVE: The aims of this study were 1) to identify the level of inflammatory biomarkers interleukin (IL)-1α, IL-1ß, IL-6, IL-8, IL-17, C-reactive protein (CRP), granulocyte colony-stimulating factor (GCSF), ferritin, and tumor necrosis factor (TNF)-α in serum and synovial fluid samples of patients who underwent revision arthroplasty surgery; 2) to establish the relationship between serum and synovial fluid levels; 3) to determine if any of the 11 genetic polymorphisms of TNFα, IL-1, IL-6, IL-8, IL-17, and GCSF on the encoding genes was associated with periprosthetic joint infection (PJI). METHODS: Synovial fluid and serum was collected from 88 patients who underwent revision arthroplasty surgery. The Musculoskeletal Infection Society definition was used to classify these patients into 2 groups: 36 PJIs and 52 aseptic failures. Synovial fluid and serum samples were tested for 9 biomarkers using a micro enzyme-linked immunosorbent assay. Genetic polymorphisms were evaluated with polymerase chain reaction and restriction endonuclease analysis. RESULTS: Synovial fluid-derived IL-1α, IL-1ß, IL-8, IL-17, CRP, GCSF, TNFα, and serum-derived IL-6, IL-17, ferritin, CRP were found suitable to classify PJI and aseptic failure. In addition, IL-17 and CRP levels demonstrated a positive correlation between synovial fluid and serum. TNFα-238, IL6-174, GCSF3R, and IL1 RN-VNTR genetic polymorphisms occurred more frequently in individuals with septic failure. CONCLUSION: Significant differences between the two groups were observed in the functional polymorphisms of the genes encoding the cytokines investigated. These differences could be interpreted as indicating that there is an association between PJI and genetic polymorphisms. LEVEL OF EVIDENCE: Level III, diagnostic study.


Assuntos
Proteína C-Reativa/análise , Ferritinas/análise , Interleucinas , Infecções Relacionadas à Prótese , Receptores de Fator Estimulador de Colônias , Líquido Sinovial/imunologia , Fator de Necrose Tumoral alfa , Artroscopia/efeitos adversos , Artroscopia/métodos , Biomarcadores/análise , Feminino , Humanos , Interleucinas/análise , Interleucinas/classificação , Interleucinas/genética , Masculino , Pessoa de Meia-Idade , Polimorfismo Genético , Infecções Relacionadas à Prótese/sangue , Infecções Relacionadas à Prótese/genética , Infecções Relacionadas à Prótese/imunologia , Receptores de Fator Estimulador de Colônias/análise , Receptores de Fator Estimulador de Colônias/genética , Reoperação/métodos , Fator de Necrose Tumoral alfa/análise , Fator de Necrose Tumoral alfa/genética
16.
Clin Exp Nephrol ; 22(1): 28-34, 2018 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-28389813

RESUMO

BACKGROUND: Iron deficiency anemia is common in patients with chronic kidney disease (CKD). Neutrophil gelatinase-associated lipocalin (NGAL), a biomarker of acute kidney injury, is known to be associated with iron metabolism. We investigated whether plasma NGAL level is associated with iron status in pre-dialysis CKD patients with anemia. METHODS: This study included 419 patients who had anemia. The subjects were into categorized into a pre-dialysis group (estimated glomerular filtration rate [eGFR] <60 ml/min/1.73 m2, n = 288) and a non-CKD group (eGFR >60 ml/min/1.73 m2, n = 131). The associations between plasma NGAL and iron status (serum ferritin and transferrin saturation [TSAT]), eGFR, albumin, uric acid, total cholesterol, calcium, phosphate, and C-reactive protein (CRP) were assessed. RESULTS: In non-CKD group, plasma NGAL was not associated with any baseline variables including iron indices (TSAT and serum ferritin). In pre-dialysis group, univariate analysis showed plasma NGAL correlated with eGFR, CRP, TSAT, and serum ferritin. In multivariate analysis, plasma NGAL was independently associated with TSAT. However, serum ferritin lost its association with plasma NGAL. In ROC analysis for identifying iron deficiency, the plasma NGAL (best cut-off value ≤394 ng/ml) was superior to the serum ferritin (suggested cut-off value ≤500 ng/ml) in both sensitivity and specificity. CONCLUSIONS: Plasma NGAL is associated with iron status in anemic patients with pre-dialysis CKD. Further studies are needed to demonstrate the role of plasma NGAL in assessing the iron deficiency and in guiding the iron therapy for pre-dialysis CKD patients.


Assuntos
Anemia Ferropriva/sangue , Ferro/sangue , Lipocalina-2/sangue , Lipocalinas/sangue , Insuficiência Renal Crônica/sangue , Adulto , Idoso , Biomarcadores/sangue , Estudos Transversais , Feminino , Ferritinas/análise , Taxa de Filtração Glomerular , Humanos , Masculino , Pessoa de Meia-Idade , Estado Nutricional , Valor Preditivo dos Testes , Curva ROC , Diálise Renal , Transferrina/análise
17.
J Sports Med Phys Fitness ; 58(10): 1490-1496, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-28597617

RESUMO

BACKGROUND: Given the likely influence that high training loads, contact-induced hemolysis and female-specific requirements have on the incidence of iron deficiency, characterizing the direction and magnitude of fluctuations in iron status over an international season is important for managing player health and physical performance in rugby sevens. METHODS: Australian national male (N.=27) and female (N.=23) rugby sevens players undertook blood tests at pre-season, mid-season, and end-season. Hemoglobin (Hb), hematocrit (Hct), ferritin, transferrin and transferrin saturation were quantified. Female athletes also reported oral contraceptive use and a subset (N.=7) provided 7-day food diaries to quantify iron intake. RESULTS: Male players typically had a three-fold higher ferritin concentration than females. Pre-season ferritin concentrations in male (151±66 µg/L) and female (51±24 µg/L) players declined substantially (~20%) by mid-season but recovered by end-season. Over the season 23% of female players were classified as iron deficient (ferritin <30 µg/L) and prescribed supplementation. The greatest incidence of iron deficiency in female players occurred mid-season (30%). Oral contraception and dietary iron intake had an unclear influence on female players' ferritin concentration, while age was largely positively correlated (r=0.66±~0.33). CONCLUSIONS: Given the relatively low ferritin concentrations evident in female rugby sevens players, and the potential for a further decline midway through a season when physical load may be at its highest, 6-monthly hematological reviews are suggested in combination with dietary management. Annual screening may be beneficial for male players, with further monitoring only when clinically indicated.


Assuntos
Futebol Americano , Ferro/sangue , Fenômenos Fisiológicos da Nutrição Esportiva , Adulto , Atletas , Austrália , Feminino , Ferritinas/análise , Hematócrito , Hemoglobinas/análise , Humanos , Ferro/deficiência , Estudos Longitudinais , Masculino , Fatores de Tempo , Transferrina/análise , Adulto Jovem
18.
Clin Biochem ; 52: 13-19, 2018 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-29051034

RESUMO

INTRODUCTION: C-reactive protein (CRP) is often elevated in patients living with severe obesity (BMI≥35kg/m2). However, there is limited information on how CRP, and other inflammation responsive biomarkers, change in response to weight loss following laparoscopic sleeve gastrectomy (LSG). We studied how CRP, ferritin and albumin change following LSG surgery in relation to obesity, metabolic syndrome (MetS) ATPIII risk components and diabetes mellitus (DM). METHODS: Laboratory parameters (including CRP) were examined in 197 patients prior to LSG, and at 6, 12, 18 and 24months. Changes in laboratory parameters, and laboratory investigations, were also examined in a 125 patient subgroup at both pre-LSG and at the 12month follow-up visit. RESULTS: All patients had BMI≥35kg/m2. CRP levels positively correlated with BMI (r=0.171, p=0.016) and alkaline phosphatase (ALP; r=0.309; P<0.001), but negatively correlated with alanine aminotransferase (ALT; r=-0.260; P<0.001) and albumin (r=-0.358; P<0.001). LSG significantly reduced CRP and ferritin, which were maintained for at least 24months. At 12months post-LSG there was a significant decrease in weight (kgs) (p<0.001), CRP (p<0.001), ferritin (p=0.004), and various MetS risk components (p<0.001) but not albumin (p=0.057). Changes in CRP also correlated with changes in weight (r=0.233, p=0.018) and ALP (r=0.208, p=0.034) but not albumin (r=-0.186, p=0.058) or ferritin (r=0.160, p=0.113) after LSG. CONCLUSION: The negative correlation between CRP and albumin levels in obesity may indicate a low grade inflammatory process affecting both. LSG related weight loss decreased CRP and ferritin, likely explained by improvement in inflammatory status.


Assuntos
Proteína C-Reativa/análise , Obesidade Mórbida/cirurgia , Adulto , Cirurgia Bariátrica , Biomarcadores/sangue , Índice de Massa Corporal , Proteína C-Reativa/metabolismo , Diabetes Mellitus , Feminino , Ferritinas/análise , Ferritinas/sangue , Seguimentos , Gastrectomia/métodos , Humanos , Inflamação/sangue , Laparoscopia/métodos , Masculino , Síndrome Metabólica , Pessoa de Meia-Idade , Obesidade/cirurgia , Período Pós-Operatório , Albumina Sérica Humana/análise , Resultado do Tratamento , Perda de Peso/fisiologia
19.
Anal Bioanal Chem ; 410(3): 933-941, 2018 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-28799040

RESUMO

We have developed multichannel integrated microfluidic devices for automated preconcentration, labeling, purification, and separation of preterm birth (PTB) biomarkers. We fabricated multilayer poly(dimethylsiloxane)-cyclic olefin copolymer (PDMS-COC) devices that perform solid-phase extraction (SPE) and microchip electrophoresis (µCE) for automated PTB biomarker analysis. The PDMS control layer had a peristaltic pump and pneumatic valves for flow control, while the PDMS fluidic layer had five input reservoirs connected to microchannels and a µCE system. The COC layers had a reversed-phase octyl methacrylate porous polymer monolith for SPE and fluorescent labeling of PTB biomarkers. We determined µCE conditions for two PTB biomarkers, ferritin (Fer) and corticotropin-releasing factor (CRF). We used these integrated microfluidic devices to preconcentrate and purify off-chip-labeled Fer and CRF in an automated fashion. Finally, we performed a fully automated on-chip analysis of unlabeled PTB biomarkers, involving SPE, labeling, and µCE separation with 1 h total analysis time. These integrated systems have strong potential to be combined with upstream immunoaffinity extraction, offering a compact sample-to-answer biomarker analysis platform. Graphical abstract Pressure-actuated integrated microfluidic devices have been developed for automated solid-phase extraction, fluorescent labeling, and microchip electrophoresis of preterm birth biomarkers.


Assuntos
Hormônio Liberador da Corticotropina/análise , Eletroforese em Microchip/instrumentação , Ferritinas/análise , Nascimento Prematuro/diagnóstico , Extração em Fase Sólida/instrumentação , Biomarcadores/análise , Hormônio Liberador da Corticotropina/sangue , Eletroforese em Microchip/métodos , Desenho de Equipamento , Ferritinas/sangue , Corantes Fluorescentes/análise , Humanos , Nascimento Prematuro/sangue , Extração em Fase Sólida/métodos
20.
Magn Reson Med ; 79(3): 1609-1615, 2018 03.
Artigo em Inglês | MEDLINE | ID: mdl-28618066

RESUMO

PURPOSE: Current MRI techniques cannot reliably assess iron content in white matter due to the confounding diamagnetic effect of myelin. The purpose of this study was to validate with histology a novel iron mapping technique that uses the temperature dependency of the paramagnetic susceptibility in multiple sclerosis (MS) brains, where white matter has been reported to show significant variations in iron content. METHODS: We investigated post mortem brain tissue from three MS patients and one control subject. Temperature-dependent R2* relaxometry was performed between 4°C and 37°C. The resulting temperature coefficient ( TcR2*) maps were compared with immunohistochemical stains for ferritin light chain. RESULTS: Good agreement between TcR2* maps and ferritin staining was found by way of visual comparison and quantitative analysis. The highest iron concentrations were detected at the edge of MS lesions and in the basal ganglia. For all regions, except the subcortical U-fibers, there was a significant negative correlation between the TcR2* values and the ferritin count. CONCLUSION: This study provides further evidence that TcR2* may be a reliable measure of white matter iron content due to the elimination of myelin-induced susceptibility changes and is well suited for further research into neurological diseases with distortions of the iron homeostasis. Magn Reson Med 79:1609-1615, 2018. © 2017 International Society for Magnetic Resonance in Medicine.


Assuntos
Encéfalo/diagnóstico por imagem , Ferritinas/análise , Imagem por Ressonância Magnética/métodos , Esclerose Múltipla/diagnóstico por imagem , Idoso de 80 Anos ou mais , Química Encefálica/fisiologia , Feminino , Humanos , Processamento de Imagem Assistida por Computador , Imuno-Histoquímica/métodos , Masculino , Pessoa de Meia-Idade , Temperatura Ambiente
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